Metabolic labeling using stable isotopes heavier stable isotope. Medical search. Frequent questions

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. (medchemexpress.com)
Stable isotope tracers (e.g., 13 C, 15 N, 18 O, and 2 H tracers), in combination with mass-spectrometry-based metabolomics, have become a widely used tool for the quantitative analysis of metabolism. (energy.gov)
The use of isotope tracers to estimate ΔG of reactions in central carbon metabolism constitutes a recent development. (energy.gov)
Quantifying ceramide kinetics in vivo using stable isotope tracers and LC-MS/MS. (omeka.net)
Enhancing Studies of Pharmacodynamic Mechanisms via Measurements of Metabolic Flux: Fundamental Concepts and Guiding Principles for Using Stable Isotope Tracers. (omeka.net)
Reisz JA, D'Alessandro A. Measurement of metabolic fluxes using stable isotope tracers in whole animals and human patients. (ucdenver.edu)
Among stable isotopes the most useful as biological tracers are the heavy isotopes of carbon and nitrogen. (uga.edu)

Heavy water, also known as deuterium oxide or deuterium water, is a compound of heavy hydrogen (D) and oxygen (O) ( Fig. 1 ). (medsci.org)
A molecule of heavy water is composed of two heavy hydrogen and one oxygen atom, with the molecular and chemical formula D 2 O. Heavy water is similar to ordinary water in appearance but has a higher density of 1.1079 g/cm 3 , as well as higher freezing and boiling points of 3.82 °C and 101.42 °C, respectively. (medsci.org)
Water is a compound of hydrogen (H) and oxygen (O), while heavy water is a compound of heavy hydrogen (D) and oxygen (O). (medsci.org)
Mechanistic model predicts tissue-environment relationships and trophic shifts in animal hydrogen and oxygen isotope ratios. (ucdenver.edu)
The IRMS Core Laboratory specializes in the measurement of natural abundance and low enrichments of stable isotopes, focusing on the light elements of hydrogen, carbon, nitrogen, and oxygen. (wisc.edu)
This session aims to bring together scientists from different fields applying single and dual oxygen and hydrogen isotope approaches on environmental-derived compounds for the reconstruction of climatic and biological processes that go beyond standard isotope analyses of water. (copernicus.org)
We also encourage people working with all techniques (IRMS-, NMR- or spectroscopy-based) to present advances in methods, as well as researchers focusing on improving oxygen and hydrogen isotope-based models to discuss their approaches. (copernicus.org)
In summary, the session will offer an overview on oxygen and hydrogen applications across different ecosystems in order to facilitate the interpretation of compound-specific isotope patterns. (copernicus.org)

For example, the medium can contain arginine labeled with six carbon-13 atoms (13C) instead of the normal carbon-12 (12C). (wikipedia.org)
A SILAC approach involving incorporation of tyrosine labeled with nine carbon-13 atoms (13C) instead of the normal carbon-12 (12C) has been utilized to study tyrosine kinase substrates in signaling pathways. (wikipedia.org)
Stable nitrogen atoms that have the same atomic number as the element nitrogen, but differ in atomic weight. (ucdenver.edu)
Atoms of the same atomic number but different atomic weights are called isotopes. (uga.edu)

For some applications, the use of isotopically labeled nutrient substrates may not be feasible due to availability and/or high cost. (energy.gov)

Nitrogen Isotopes" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)
N-15 is a stable nitrogen isotope. (ucdenver.edu)
This graph shows the total number of publications written about "Nitrogen Isotopes" by people in this website by year, and whether "Nitrogen Isotopes" was a major or minor topic of these publications. (ucdenver.edu)
Below are the most recent publications written about "Nitrogen Isotopes" by people in Profiles. (ucdenver.edu)
Each has a heavy isotope ( 13 C and 15 N) with a natural abundance of ~1% or less and a light isotope ( l2 C and 14 N) that makes up all of the remainder, in the case of nitrogen, or virtually all in the case of carbon (carbon also has a radioactive isotope, 14 C. (uga.edu)
We can analyze any parts of plants for nitrogen and carbon stable isotopes and total content. (uga.edu)
We analyze all types of soils for nitrogen and carbon stable isotopes and total content. (uga.edu)
unfortunately, the use of carbon isotope C-13 or the nitrogen isotope N-15 is associated with certain disadvantages. (uni-mainz.de)

Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. (wikipedia.org)
Pulsed SILAC (pSILAC) is a variation of the SILAC method where the labelled amino acids are added to the growth medium for only a short period of time. (wikipedia.org)
It had also been used to study biofilm tolerance to antibiotics to differentiate tolerant and sensitive subpopulations Traditionally the level of multiplexing in SILAC was limited due to the number of SILAC isotopes available. (wikipedia.org)
Recently, a new technique called NeuCode (neutron encoding) SILAC, has augmented the level of multiplexing achievable with metabolic labeling (up to 4). (wikipedia.org)
The NeuCode amino acid method is similar to SILAC but differs in that the labeling only utilizes heavy amino acids. (wikipedia.org)
The use of only heavy amino acids eliminates the need for 100% incorporation of amino acids needed for SILAC. (wikipedia.org)
SILAC RPMI is optimized for labeling experiments involving the use of stable amino acid isotopes in cell culture to analyze protein expression by mass spectrometry (MS). (capricorn-scientific.com)
SILAC RPMI 1640, w/o L-Arginine, w/o L-Lysine, w/o L-Glutamine, w/o Phenol Red is optimized for labeling experiments involving the use of stable amino acid isotopes (SILAC = stable isotope labeling with amino acids in cell culture). (capricorn-scientific.com)
SILAC labeling is accomplished via normal metabolic processes (e.g., cell division), by incorporating non-radioactive stable amino acid isotopes into newly synthesized proteins. (capricorn-scientific.com)
SILAC RPMI is formulated without L-arginine and L-lysine for multiple isotopic amino acid labeling and has no effect on cell morphology or growth rates. (capricorn-scientific.com)

As a new quantitative method, heavy water-labeled single-cell Raman microspectroscopy can reduce damage to cells and human interference factors. (medsci.org)
This method does not alter the cell composition or rely on cell culture and thus allows the rapid, quantitative, and nondestructive evaluation of the effect of drugs on the real-time metabolic activity of microorganisms at the single-cell level [ 8 - 10 ]. (medsci.org)
These MS-based quantitative approaches using label-free, tags, or stable isotope labeling have their own strengths and limitations. (openaccesspub.org)
Quantitative proteomic methods fall into two general categories: label-based or label-free quantification. (openaccesspub.org)

In this work, the use of deuterated water (also named as "heavy water," 2 H 2 O, or D 2 O) is explored as a non-nutrient, substrate-agnostic, cost-effective isotope tracer for investigating reversibility of reactions in central carbon metabolism. (energy.gov)
A Single-Carbon Stable Isotope Ratio Model Prediction Equation Can Estimate Self-Reported Added Sugars Intake in an Adult Population Living in Southwest Virginia. (ucdenver.edu)
Carbon-13-labelled fumarate is already a molecule that plays a significant role in hyperpolarized imaging. (uni-mainz.de)

Culp-Hill R, Reisz JA, Hansen KC, D'Alessandro A. Investigation of the effects of storage and freezing on mixes of heavy-labeled metabolite and amino acid standards. (ucdenver.edu)

The aim of this study was to monitor seasonal changes in stable isotopes of pool freshwater and harp seal ( Phoca groenlandica ) body water, and to study whether these potential seasonal changes might bias results obtained using the doubly labelled water (DLW) method when measuring energy expenditure in animals with access to freshwater. (biologists.com)
We have pioneered the groundbreaking methodology of doubly-labeled water that provides valuable insights into various aspects of nutrition and energy metabolism. (wisc.edu)
We employ the technique of doubly labeled water to accurately measure energy expenditure in living organisms. (wisc.edu)

The dataset contains over 1.92 million data points in protein isotope labeling kinetics, culminating as the in vivo turnover rates of 3,228 cardiac proteins and the expression levels of 8,064 proteins. (nature.com)

These labeled target proteins can also be used for protein quantification. (capricorn-scientific.com)
isotope labeled heavy vs. light quantification (e.g. (princeton.edu)

Leveraging unlabeled, natural abundance isotope ratio measurements, we conduct food web analyses to unravel complex ecological relationships. (wisc.edu)

Proteins from both cell populations are combined and analyzed together by mass spectrometry as pairs of chemically identical peptides of different stable-isotope composition can be differentiated in a mass spectrometer owing to their mass difference. (wikipedia.org)
Protein levels are measured with a mass spectrometer, based on signal intensity (labeled cells appear heavier). (capricorn-scientific.com)

Steady-state and dynamic isotope tracer experiments can provide information on metabolic network structure, metabolic fluxes, and thermodynamics of metabolic reactions and pathways. (energy.gov)
We anticipate these data will aid in understanding key mitochondrial and metabolic pathways in heart diseases, and further serve as a reference for methodology development in dynamics studies in multiple organ systems. (nature.com)
Key proteins in mitochondria and metabolic pathways are encompassed, in addition to contractile machineries and sarcolemmal signaling proteins. (nature.com)

Through our precise measurements, we determine the relative abundances of isotopes such as 2 H/ 1 H, 13 HC/ 12 C, 15 N/ 14 N, and 18 O/ 16 O. Our instruments possess exceptional precision, allowing us to detect part-per-million differences in heavy isotope abundances. (wisc.edu)

The seals were offered daily amounts of capelin and kept under a seasonal photoperiod of 69°N. Large seasonal variations of deuterium and oxygen-18 in the pool water were measured, and the isotope abundance in the body water showed similar seasonal changes to the pool water. (biologists.com)
Most elements of biological interest (including C, H, O, N, and S) have two or more stable isotopes, with the lightest of these present in much greater abundance than the others. (uga.edu)

Integrate advanced mass spectrometry, computational modeling, and metabolic engineering to develop an experimental-computational approach for the in vivo genome-scale determination of Gibbs free energies ( ΔG ) in metabolic networks suitable for high-throughput thermodynamic profiling of engineered organisms and emerging model systems. (energy.gov)
Using an integrated platform of metabolic labeling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the in vivo half-life of 3,228 and the expression of 8,064 cardiac proteins, quantified under healthy and hypertrophic conditions across six mouse genetic strains commonly employed in biomedical research. (nature.com)

One salient example of this is metabolic flux analysis in industrially relevant cellulolytic microbes, such as Clostridium thermocellum , that metabolize complex substrates (i.e., lignocellulosic biomass). (energy.gov)
Through stable isotope labeling, we investigate the oxidation of energy substrates in living systems. (wisc.edu)

When the cells are growing in this medium, they incorporate the heavy arginine into all of their proteins. (wikipedia.org)
Cells growing in this medium take up the heavy amino acids and enable the differentiation between light and heavy proteins. (capricorn-scientific.com)
We investigated the entire set of proteins modulated by BAG3 silencing in the human anaplastic thyroid 8505C cancer cells by using the Stable-Isotope Labeling by Amino acids in Cell culture strategy combined with mass spectrometry analysis. (oncotarget.com)

Welcome to the Isotope Ratio Mass Spectrometry (IRMS) Laboratory, a premier research facility dedicated to the precise measurement of stable isotopes in various samples. (wisc.edu)

Troglitazone-d 4 is deuterium labeled Troglitazone. (medchemexpress.com)
Febuxostat-d 7 is deuterium labeled Febuxostat. (medchemexpress.com)
Variations in background levels of deuterium and oxygen-18 in freshwater sources may be due to seasonal changes in physical processes such as precipitation and evaporation that cause fractionation of isotopes. (biologists.com)

As a new type of probe, the heavy isotope of water has been widely used in the study of cell development, metabolism, tissue homeostasis, aging, and tumor heterogeneity. (medsci.org)
Herein, we review findings supporting the applications of and research on heavy water in monitoring of bacterial metabolism, rapid detection of drug sensitivity, identification of tumor cells, precision medicine, and evaluation of skin barrier function and promote the use of heavy water as a suitable marker for the development of detection and treatment methodologies. (medsci.org)
By using stable isotopes, we can precisely determine the rate at which individuals consume energy, allowing for a comprehensive understanding of energy balance and metabolism. (wisc.edu)
This methodology enables us to elucidate the utilization and metabolic fate of different energy sources, shedding light on the intricate processes involved in energy metabolism. (wisc.edu)

We offer pre-isotope analysis lipid and urea extraction for diet and tracer studies. (uga.edu)

This technique allows non-destructive, label-free spectral imaging and analysis of cells, tissues, and nanoparticles [ 5 - 7 ]. (medsci.org)
Walter BS, DeWitte SN, Dupras T, Beaumont J. Assessment of nutritional stress in famine burials using stable isotope analysis. (ucdenver.edu)
Redfern RC, DeWitte SN, Beaumont J, Millard AR, Hamlin C. A new method for investigating the relationship between diet and mortality: hazard analysis using dietary isotopes. (ucdenver.edu)
It is therefore strongly recommended that analysis of seasonal changes in background levels of isotopes is performed before the DLW method is applied on (free-ranging) animals, and to use a control group in order to correct for changes in background levels. (biologists.com)
In the IRMS Laboratory, our dedicated team of scientists and researchers are at the forefront of stable isotope analysis in biotechnology and nutritional sciences. (wisc.edu)

The hyperpolarized fumarate is converted into malate by the addition of a heavy water molecule. (uni-mainz.de)

Stable-isotope labeling is a powerful tool widely used in most MS-based assays to investigate the topology and operation of metabolic networks. (environmental-expert.com)

Rate constant estimation with heavy water requires a long-term experiment with data collection at multiple time points (3-4 weeks for mitochondrial proteome dynamics in mice and much longer in other species). (omeka.net)

The Stable Isotope Ecology Laboratory of the Center for Applied Isotope Studies at the University of Georgia , offers a wide variety of analytical services to researchers associated with any bona-fide nonprofit research organization. (uga.edu)

Researchers at Johannes Gutenberg University Mainz (JGU) and the Helmholtz Institute Mainz (HIM) have now unveiled a new technique for observing metabolic processes in the body. (uni-mainz.de)
It is possible to record images of the hyperpolarized signal at different points in time, which enables the real-time tracking of metabolic processes. (uni-mainz.de)

Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs [1] . (medchemexpress.com)

Currently, the commonly used methods to quantify heavy water concentration include the density method, mass spectrometry, and spectroscopy techniques [ 3 ]. (medsci.org)

In contrast, the second population is fed with growth medium containing amino acids labeled with stable (non-radioactive) heavy isotopes. (wikipedia.org)
The increased multiplexing capability of NeuCode amino acids is from the use of mass defects from extra neutrons in the stable isotopes. (wikipedia.org)
In this process, the "light" amino acids contained in the growth medium are replaced by "heavy" ones. (capricorn-scientific.com)

Heavy water is an ideal contrast agent for metabolic activity and can be adapted to a wide range of biological systems owing to its non-invasiveness, universal applicability, and cost-effectiveness. (medsci.org)

At the heart of our laboratory's expertise lies our profound understanding and extensive experience in nutritional applications of stable isotopes. (wisc.edu)

Heavy water has been widely used as a probe in many fields. (medsci.org)

Here, we assessed the fate of lignin and non-cellulosic carbohydrates in density fractionated soils (light fraction, LF vs. heavy fraction, HF) from three permafrost regions with decreasing continentality, expanding from east to west of northern Siberia (Cherskiy, Logata, Tazovskiy, respectively). (univie.ac.at)

This work will aid in the construction of accurate metabolic models that incorporate thermodynamic constraints and guide fast rational engineering of microbial networks. (energy.gov)

Wang X, Liu Nm, Zhao Yf, Yang F, Zhu Zj, Song D. Research Progress in the Medical Application of Heavy Water, Especially in the Field of D 2 O-Raman Spectroscopy. (medsci.org)
The relative molecular mass of heavy water, 20.0275 Mr, is higher than that of water (H 2 O, 18.0153 Mr) by about 11%, hence the name "heavy" water. (medsci.org)
Hence, the chemical properties of heavy water and ordinary water are also very similar. (medsci.org)
It has been proved that a small amount of heavy water does not cause adverse reactions [ 1 - 2 ]. (medsci.org)

Elements can exist in both stable and unstable (radioactive) forms. (uga.edu)

In our contribution, we discuss the potential, the advances and the limitation of the coupled isotope approach based on leaf wax-derived n -alkane and hemicellulose-derived sugar biomarkers (δ 2 H n -alkanes - δ 18 O sugars ). (copernicus.org)

Thereafter, all peptides containing a single arginine are 6 Da heavier than their normal counterparts. (wikipedia.org)

In an effort to meet the growing demands of scientific research and various industries, the ISO 9001:2015 certified chemical supplier Alfa Chemistry has expanded its range of isotope-labeled products and now offers a wider range of isotope-labeled polymers, buffers and reagents, flavors and fragrances, and metal stable isotopes. (environmental-expert.com)

In this study, we selected one mining site restored in 2007, and we used DNA stable isotope probing (DNA-SIP) to identify which bacteria grow in post-mining and adjacent natural biocrusts. (copernicus.org)

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