Mesodermal cells hematopoietic stem cells embryonic cell differentiation. Medical search. Frequent questions

The hematopoietic and endothelial lineages derive from mesoderm and are thought to develop through the maturation of a common progenitor, the hemangioblast. (elsevierpure.com)
Single-cell transcriptomic analysis confirmed inhibition of lineage-committed progenitor cells in physioxic cultures. (stanford.edu)
Apelin receptor (Aplnr/Agtrl1/Apj) marks a transient cell population during the differentiation of hematopoietic stem and progenitor cells (HSPCs) from pluripotent stem cells but its function during the production and maintenance of haematopoietic stem cells is not clear. (ed.ac.uk)
Recently, stage-specific embryonic kidney progenitor tissue was used to regenerate nephrons. (tau.ac.il)
Through fluorescence-activated cell sorting, microarray analysis, in vitro differentiation assays, mixed lymphocyte reaction, and a model of ischemic kidney injury, this study sought to identify and characterize multipotent organ stem/progenitor cells in the adult kidney. (tau.ac.il)
Although clonal studies of lineage potential have been extensively applied to organ specific stem and progenitor cells, much less is known about the clonal origins of lineages formed from the germ layers in early embryogenesis. (ca.gov)
In contrast to studies that have used VISA to track differentiation of self-renewing stem cell clones that amplify significantly over time, we focused on a population of progenitor clones with limited self-renewal capability. (ca.gov)
In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. (thermofisher.com)
In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
Angioblast, the endothelial progenitor, is generated from hemangioblasts that are derived from the Flk1+ mesodermal cells, or directly from the Flk1+ mesodermal cells. (chinagene.cn)
Runx1 is critically required along the transition of HE to definitive haematopoietic stem and progenitor cells, and several of its known transcriptional targets act in this transition. (ox.ac.uk)
The 23GFP expression marks all HE and emerging haematopoietic stem and progenitor cells, similar to endogenous Runx1. (ox.ac.uk)
Though not defined as a hematopoietic organ, the lung houses many resident hematopoietic cells, aids in platelet biogenesis, and is a reservoir for hematopoietic stem and progenitor cells (HSPCs). (bu.edu)
We studied the secretome profile of gestational tissue-derived MSCs and their effects on functions of endothelial progenitor cells (EPCs), another angiogenic cell type that plays an important role during the neovascularization. (hindawi.com)
Mesenchymal stem cells (MSCs) are multipotent stem/progenitor cells which can differentiate to several mesodermal derivatives and possess an ability to secrete factors involved in neovascularization and immunomodulation [ 1 , 2 ]. (hindawi.com)
However, recent studies have demonstrated that other angiogenic cells called endothelial progenitor cells (EPCs) also play an important role during the formation of new vessels through the process of vasculogenesis [ 7 - 9 ]. (hindawi.com)
At first, the mesenchymal stem cell was thought to be the primary component of this undifferentiated cell type, however it is now evident that within the adipose extracellular matrix are also adipocytic precursors (known as progenitor cells) adherent to adipocytes, and in close approximation to a variety of additional undifferentiated multipotent and pluripotent cells, including pericytes and endothelial cells, all thought to play important roles in mesenchymal-stromal derived tissue regeneration. (journalofprolotherapy.com)
CC-ASC generated epithelial cells with undifferentiated morphology in all culture methods, among which CC-ASC in 3D culture supported the highest cell doubling (cells doubled 9.0 times compared to cells doubled 4.9 times in control) while maintained the percentage of putative limbal stem/progenitor cells compared to the control. (drcremers.com)
Mei H, González S, Nakatsu MN, Baclagon ER, Chen FV, Deng SX (2017) Human adipose-derived stem cells support the growth of limbal stem/progenitor cells. (drcremers.com)
In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)
hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)
Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
The EBF transcription factor Collier directly promotes Drosophila blood cell progenitor maintenance independently of the niche. (sdbonline.org)
The maintenance of stem or progenitor cell fate relies on intrinsic factors as well as local cues from the cellular microenvironment and systemic signaling. (sdbonline.org)
Together, this study shows that the PSC is dispensable for blood cell progenitor maintenance and reveals the key role of the conserved transcription factor Col as an intrinsic regulator of hematopoietic progenitor fate. (sdbonline.org)
They also showed that cross-regulation between col and eya in muscle progenitor cells is required for specification of muscle identity, revealing a new parallel between the myogenic regulatory networks operating in Drosophila and vertebrates. (sdbonline.org)

After the in vitro differentiation of these embryonic stem cells to embryoid bodies, developing mesodermal progenitors could be separated from those with neuroectoderm potential based on GFP expression. (elsevierpure.com)
During embryonic development mesodermal differentiate into endothelial cells, hemangioblast and hematopoietic progenitors give rise to blood vessel, the vasculogenesis. (samploon.com)
Genetic Tagging During Human Mesoderm Differentiation Reveals Tripotent Lateral Plate Mesodermal Progenitors. (ca.gov)
By applying a quantitative framework to estimate the degree of undersampling we revealed the existence of tripotent mesodermal progenitors derived from pluripotent stem cells, and the subsequent bifurcation of their differentiation into bipotent endothelial/hematopoietic or endothelial/mesenchymal progenitors. (ca.gov)
Ablation of Rpl22l1 in late fetal liver progenitors impairs the development of B lineage progenitors at the pre-B stage and development of T cells at the CD44-CD25+ double-negative stage. (bvsalud.org)
Mesodermal precursors expressing PDGFRα appear transiently during E7.5-8.5 descend to a subset of Lin- Sca1+ Kit+ hematopoietic progenitors found in adult BM. (bvsalud.org)
The T-box transcription factor Eomesodermin governs haemogenic competence of yolk sac mesodermal progenitors. (ox.ac.uk)
Hemogenic endothelial cells (HECs) are specialized cells that undergo endothelial-to-hematopoietic transition (EHT) to give rise to the earliest precursors of hematopoietic progenitors that will eventually sustain hematopoiesis throughout the lifetime of an organism. (bu.edu)
In the lymph gland , an hematopoietic organ in Drosophila larva, a group of cells called the Posterior Signaling Centre (PSC), whose specification depends on the EBF transcription factor Collier (Col) and the HOX factor Antennapedia (Antp), has been proposed to form a niche required to maintain the pool of hematopoietic progenitors (prohemocytes). (sdbonline.org)
In contrast with this model, this study shows that genetic ablation of the PSC does not cause an increase in blood cell differentiation or a loss of blood cell progenitors. (sdbonline.org)
Collier is expressed in a core population of lymph gland progenitors and cell autonomously maintains this population. (sdbonline.org)
The PSC contributes to lymph gland homeostasis by regulating blood cell differentiation, rather than by maintaining core progenitors. (sdbonline.org)

In 1997, Keller's team was the first to successfully isolate the developmentally significant hemangioblast-a multipotent precursor cell that can differentiate to the hematopoietic and endothelial cell fates-using embryonic stem cell technology. (wikipedia.org)
Likewise, while the number of transplanted HSCs is well known to directly impact engraftment efficiency, there are currently no established clinical protocols to successfully expand donor-harvested HSCs, nor to differentiate embryonic or induced pluripotent stem cells (iPSCs) into functional HSCs in vitro. (grantome.com)
Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
Mesenchymal stem cells (MSCs), or stromal stem cells, can differentiate into many different types of … In contrast, treatments that utilize MSCs from a patientâ s fat (adipose) sample have shown weak or unreliable responses. (tomeipowered.com)
Concomitantly, the ability of MSCs to differentiate and give rise to multiple cell types such as adipocytes, chondrocytes, and osteoblasts … These transplants vastly increase the bodyâ s natural healing abilities and have potent anti-inflammatory and immunosuppressive responses.Â. (tomeipowered.com)
Mesenchymal Stem Cells Instruction Manual Product Description Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are self-renewing multipotent cells that can differentiate into a wide variety of cell types. (tomeipowered.com)
For example, hematopoietic stem cells differentiate into various immune cells. (cusabio.com)
while p38 kinase inhibited cell develop into ectodermal cells and eventually differentiate into neural cells. (cusabio.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
1. Stem cells have long been known to have the ability to differentiate (ie become/transform) into "good/normal" tissue. (drcremers.com)
Human embryonic stem cells (hESCs) derived from an early embryo can self-renew indefinitely and differentiate into a variety of cell types. (ashpublications.org)

Especially Mesenchymal Stem cells have been focused in a plethora of preclinical and clinical experiments over the five decades due to extraordinary capacity in differentiation to multiple lineages, immunomodulation and angigenic properties. (samploon.com)
Large body of data of experiment concludes that EMSCs have the ability to differentiation in multiple lineages such as osteoblast, cartilage, adipose, smooth muscle cell(1/18), hepatocytes, cardiomyocyte, neural cells. (samploon.com)
Global gene profiling reveals enrichment for many genes downstream of developmental signaling molecules and self-renewal pathways, such as TGF-β/bone morphogenic protein, Wnt, or fibroblast growth factor, as well as for those that are involved in specification of mesodermal lineages (myocyte enhancer factor 2A, YY1-associated factor 2, and filamin-β). (tau.ac.il)
We applied lentiviral tagging followed by vector integration site analysis (VISA) with high-throughput sequencing to investigate the ontogeny of the hematopoietic, endothelial and mesenchymal lineages as they emerge from human embryonic mesoderm. (ca.gov)
We have used RT-PCR to screen pluripotent murine embryonic stem cells to identify receptor tyrosine kinases (RTKs) potentially involved in the determination or differentiation of cell lineages during early mouse development. (silverchair.com)
osteoblasts, chondrocytes, endothelial-cells and also non-mesoderm-type lineages e.g. (tomeipowered.com)
Ligand-dependent development of the en-dothelial and hemopoietic lineages from embryonic mesodermal cells expressing vascular endothelial growth factor receptor 2. (chinagene.cn)
Conditional loss of murine Rpl22l1 using stage or lineage-restricted Cre drivers impairs development of several hematopoietic lineages. (bvsalud.org)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)

Identification of genes involved in in vitro differentiation induction of embryonic stem cells (ESCs) into hematopoietic stem cells (HSCs) has been challenged during last decade. (ebiotrade.com)
These observations imply that current in vitro differentiation strategies are missing critical cues which are essential to unlock or maintain full HSC potential in vivo. (grantome.com)

Hematopoietic stem cells (HSCs) are a rare hematopoietic cell type that can entirely reconstitute the blood and immune systems following transplantation. (stanford.edu)
Hematopoietic stem cells (HSCs), first produced in the developing vertebrate embryo, supply the lifelong foundation of the blood and immune systems. (grantome.com)
Therefore, the identification of novel modifiers of de novo production of HSCs with long-term self-renewal and differentiation capacity is a major unmet clinical need. (grantome.com)
In the developing embryo, definitive HSCs arise from a unique population of mesodermal precursors termed hemogenic endothelium (HEC) through a process known as endothelial-to-hematopoietic transition (EHT). (grantome.com)
Defining the molecular signaling pathways that mediate productive HSC formation in vivo will reveal new targets for optimizing the directed expansion and/or differentiation of adult-type HSCs for clinical use. (grantome.com)
Hematopoietic stem cells (HSCs), produced in the developing embryo, form the foundation of our blood system. (grantome.com)
Unlike traditional, circulating lymphocytes that are continuously generated from hematopoietic stem cells (HSCs), many TLCs are of fetal origin and poorly generated from adult HSCs. (bvsalud.org)
Haematopoietic stem cells (HSCs) have been used in the clinic for several decades. (ox.ac.uk)
Yet, our capacity to grow, expand and manipulate HSCs is still limited, which is holding back a more wider application of HSC-based therapies, for example in therapies aimed at correcting single gene haematopoietic disorders by genome editing. (ox.ac.uk)
The Developmental Haematopoiesis Group aims to learn from the embryo how HSCs are specified and how their self-renewal and differentiation into functional end cells is controlled, and apply this knowledge for the purpose of regenerative medicine. (ox.ac.uk)
Making use of this reporter in in vivo HSC development and ESC/iPSC differentiation cultures, our goal is to further the understanding of how HSCs are specified and born. (ox.ac.uk)
Adult stem cells comprise mesenchymal stem cells (MSCs), hematopoietic stem cells (HSCs) and ectodermal stem cells (ESCs). (biomedcentral.com)

as well as to mesodermal cell fates: cardiomyocytes, chondrocytes, T lymphocytes and myeloid precursors. (wikipedia.org)
Multiple embryonic precursors give rise to leukocytes in adults while the lineage-based functional impacts are underappreciated. (bvsalud.org)
and Tracing the origin of HE back to gastrulation to begin to map the factors acting on the precursors of these cells. (ox.ac.uk)
Depletion of Runx1 with antisense morpholino oligonucleotides abrogated the development of both blood and vessels, as demonstrated by loss of circulation, incomplete development of vasculature and the accumulation of immature hematopoietic precursors. (biologists.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)

Gordon M. Keller is a Canadian scientist recognized for his research on applying developmental biology findings to in vitro pluripotent stem cell differentiation. (wikipedia.org)
Transduction of Lhx2 into induced pluripotent stem cells (iPSCs) was also effective in generating KSL cells in vitro, as well as HSC-like activities in vivo. (ebiotrade.com)
Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. (stanford.edu)
Specifically, about the paracrine activities of amniotic fluid stem cells, renal stem cells, embryonic stem cells, mesenchymal stem cell, induced pluripotent stem cells as well as other stem cells. (biomedcentral.com)
However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)
The Center for Regenerative Medicine (CReM) is a joint effort between Boston University and Boston Medical Center that brings together nine principal investigators addressing various aspects of developmental biology, stem cells, regeneration and injury, cell lineage specification and disease modeling with a major focus on induced Pluripotent Stem Cells or iPSCs. (bu.edu)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Differentiation of human pluripotent stem cells (hPSCs) into functional cell types is a crucial step in cell therapy. (ashpublications.org)

Transduction of Lhx2 into ESC-derived mesodermal cells resulted in robust differentiation of c-Kit+/Sca-1+/Lineage- (KSL) cells in vitro. (ebiotrade.com)
Furthermore, transplantation of Lhx2-transduced hematopoietic cells into lethally irradiated mice resulted in multi-lineage repopulation of hematopoietic cells over 4 months. (ebiotrade.com)
Mesenchymal stem cells (MSCs) are widely regarded to be promising cell sources for bone tissue engineering, due to their multi-lineage differentiation potential and extensive self-renewal capacity [ 5 ]. (biomedcentral.com)
This population of small cells includes a CD45-negative fraction that lacks hematopoietic stem cell and lineage markers and resides in the renal interstitial space. (tau.ac.il)
Transcriptional activity is regulated by DNA binding transcription factors, but these typically act in more than one cell lineage activating different subsets of target genes in each. (edu.au)
Their cell lineage-specific effects are governed by chromatin structure. (edu.au)
Using Flk2- and Il7r-Cre lineage tracing, we found that peritoneal B1a cells, splenic marginal zone B (MZB) cells, lung ILC2s and regulatory T cells (Tregs) were highly labeled. (bvsalud.org)
Egress of PDGFRα-lineage-rich monocytes and subsequent differentiation to peripheral macrophages spatially segregates them from non-PDGFRα-lineage BM-resident macrophages and allows functional specialization since macrophages derived from these egressing monocytes differ in morphology, phenotype, and functionality from BM-resident macrophages in culture. (bvsalud.org)

13) Like other sources, endometrial MSCs have recently been emerged as a promising alternative source of therapeutic MSCs due to its unique ability to undergo smooth muscle differentiation, migratory, angiogenic. (samploon.com)
As a type of MSCs derived from dental tissues, human dental pulp stem cells (hDPSCs) are easily and readily isolated from extracted teeth. (biomedcentral.com)
As a result of over five decades of investigation, mesenchymal stromal/stem cells (MSCs) have emerged as a versatile and frequently utilized cell source in the fields of … MSC have been shown to differ-entiate in vitro into adipocytes, chon-drocytes, osteoblasts, myocytes, and ß-pancreatic islets cells. (tomeipowered.com)
The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. (tomeipowered.com)
The inherent tumor-tropic property of MSCs can be used to target cancer cells. (tomeipowered.com)
Mesenchymal stem cells (MSCs) are an example of tissue or 'adult' stem cells. (tomeipowered.com)
MSCs make the different specialized cells found in the skeletal tissues. (tomeipowered.com)
The original mesengenic process pathway (Figure 1) was hypothesized and fashioned … Two opposing descriptions of so-called mesenchymal stem cells (MSCs) exist at this time. (tomeipowered.com)
The problem is what a mesenchymal stem cell (MSCs) is isn't always clearly defined, which has led to confusion in the scientific community, as well as public misunderstandings exploited by businesses marketing questionable cell-based treatments, the authors argue. (tomeipowered.com)
Properties of Mesenchymal … We found that … Platelets are known to enhance the wound-healing activity of mesenchymal stem cells (MSCs). (tomeipowered.com)
Among the cell types that can be used for this purpose, mesenchymal stem cells (MSCs) are considered as promising source of stem cells in personalized cell-based therapies. (tomeipowered.com)
Mesenchymal stem/stromal cells (MSCs) are multipotent cells that are emerging as the most promising means of allogeneic cell therapy. (tomeipowered.com)
The ERK-MAPK signaling pathway plays a role in the differentiation of mesenchymal stem cells (MSCs) into adipocytes [1] [2] . (cusabio.com)
p38 pathway plays a very important role in the osteogenic differentiation of mesenchymal stem cells (MSCs). (cusabio.com)
Among them, Runx2 is a key target gene affecting osteogenic activity, and Cbfa1 regulates the differentiation of MSCs into osteoblasts at the transcriptional level. (cusabio.com)
Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
Therapeutic potentials of mesenchymal stem cells (MSCs) depend largely on their ability to secrete cytokines or factors that modulate immune response, enhance cell survival, and induce neovascularization in the target tissues. (hindawi.com)
Previous studies demonstrated that MSCs could ameliorate the pathology associated with ischemic heart disease, ischemic stroke, and peripheral vascular disease by releasing several critical factors that increase cell survival, provide an appropriate microenvironment for repairing damaged tissue, and induce neovascularization [ 3 - 5 ]. (hindawi.com)
However, recent studies showed that MSCs derived from distinct sources might produce distinct combination of factors that exert different effects on target cells [ 15 , 16 ]. (hindawi.com)

EnSCs synthesize and discharge several growth factors including transforming growth factor-α (TGFα), epidermal growth factor (EGF) and insulin-like growth factor-I (IGF-I). IGF-I, TGFα, epidermal growth factor receptor (EGFR), basic fibroblast growth factor (bFGF), and platelet derived growth factor-β (PDGFβ) receptor expression in epithelial cells are upregulated during expeditious growth in the proliferative stage. (samploon.com)
To optimize the ASC-supported culture, freshly isolated limbal epithelial cells in the form of single cells (SC-ASC) or cell clusters (CC-ASC) were cultured using three different methods: LSCs seeded directly on feeder cells, a 3-dimensional (3D) culture system and a 3D culture system with fibrin (fibrin 3D). (drcremers.com)

The self-renewal and proliferative nature of stem cells raised the hope to fight against various diseases. (biomedcentral.com)
In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)

We portrayed the Notch system in embryonic stem cell (ESC)-derived embryoid bodies (EBs) differentiating under the standard protocols used to assess yolk sac (YS) hematopoiesis in vitro. (karger.com)
A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. (thermofisher.com)

Long-term physioxic expansion also afforded culture-based ex vivo HSC selection from whole bone marrow, spleen, and embryonic tissues. (stanford.edu)
However, the accurate quantitation of mixed cell types in chimeric and mosaic tissues is complicated by sample preparation bias, transgenic silencing, phenotypic similarity, and low-throughput analytical pipelines. (stanford.edu)
Cells Tissues Organs (2011) 193 (4): 239-252. (karger.com)
Recognition of the vast number of undifferentiated cells associated with the stromal vascular fraction has resulted in extensive research demonstrating the heterogeneity of such cells, and their ability to participate in production of all mesodermal-derived tissues. (journalofprolotherapy.com)
Here is a general list of all the Ways Stem Cells heal tissues or create new tissue. (drcremers.com)
Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)

In 2016, Keller co-founded Toronto-based BlueRock Therapeutics with Lorenz Studer for the development of iPSC-derived cells for clinical transplantation. (wikipedia.org)
This article will explain what Haematopoietic stem cell transplantation (HSCT) involves, its benefits/risks, and how it may or may not be helpful for certain neurological conditions. (tomeipowered.com)
In contrast, loss of IL7Rα, or combined deletion of Flk2 and IL7Rα, dramatically reduced the number of B1a cells, MZBs, ILC2s and Tregs, both in situ and upon transplantation, indicating an intrinsic and essential role for IL7Rα. (bvsalud.org)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. (drcremers.com)

To investigate the developmental processes that regulate mesoderm induction and specification to the hemangioblast, we generated an embryonic stem cell line with the green fluorescent protein (GFP) targeted to the mesodermal gene, brachyury. (elsevierpure.com)
Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. (stanford.edu)
The diverse cell types are genetically identical, but differ in their form and function due to differences in gene expression. (edu.au)
Therefore, gene activity is an important mechanism in embryonic development and adult stem cell differentiation. (edu.au)
Griscelli syndrome type 2 (GS-2) is a rare, autosomal recessive immune deficiency syndrome caused by a mutation in the RAB27A gene, which results in the absence of a protein involved in vesicle trafficking and consequent loss of function of in particular cytotoxic T and NK cells. (biomedcentral.com)
All GS-2 iPSC clones displayed a normal karyotype (46XX or 46XY) and were shown to express the same RAB27A gene mutation that was present in the original somatic donor cells. (biomedcentral.com)
This complex process is regulated by the interplay of transcription factors with a chromatin environment, both of which provide the epigenetic information maintaining cell-type specific gene expression patterns. (amegroups.org)
The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
Embryonic development is tightly controlled at the level of gene expression. (amegroups.org)
In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)
scMEGA: single-cell multi-omic enhancer-based gene regulatory network inference. (costalab.org)

Tissue engineering and cell therapy approaches aim to take advantage of the repopulating ability and plasticity of multipotent stem cells to regenerate lost or diseased tissue. (tau.ac.il)
With high levels of platelet-derived growth factors and cytokines, this combination provides both a living bioscaffold and a multipotent cell replenishment source useful for enhanced musculoskeletal healing. (journalofprolotherapy.com)

Our prior work revealed that Runx1 expression is strongly upregulated after initiation of the embryonic heartbeat, and HSC production is coordinated with the onset of vigorous circulatory flow and sheer stress. (grantome.com)
In mice, homozygous loss of Runx1 results in embryonic lethality and a complete absence of definitive haematopoietic cells. (ox.ac.uk)
To facilitate the identification and isolation of HE and cells undergoing EHT for mechanistic studies, we generated a Runx1 +23 enhancer-reporter model (23GFP). (ox.ac.uk)
Zebrafish runx1 is expressed in hematopoietic and neuronal cells during early embryogenesis. (biologists.com)
runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl , and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche . (biologists.com)
Transient expression of runx1 in cloche embryos resulted in partial rescue of the hematopoietic defect. (biologists.com)

EMSCs also have the high capacity proliferation, multidirectional differentiation that is indicative for the potentiality for tissue regeneration. (samploon.com)
Angiogenesis starts from local destruction of the wall of preexisting blood vessel, activation of endothelial cell proliferation, and migration. (samploon.com)
MAPK can cause the activation of transcription factors in the nucleus, participate in the process of signal transmission from the cell surface to the nucleus, and regulate cell proliferation and differentiation. (cusabio.com)
This pathway can also affect the proliferation and differentiation of red blood cells. (cusabio.com)
Studies have shown that ERK signaling pathway is also involved in signal transduction of osteoblast differentiation and proliferation. (cusabio.com)
WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
For several decades, neovascularization is believed to be accomplished by proliferation of mature endothelial cells residing in the local vessels through the process of angiogenesis [ 6 ]. (hindawi.com)

While mechanical properties of the niche, including sheer stress and circumferential stretch, are increasingly recognized as important stem cell cues in many contexts, the mechanism(s) by which mechanotransduction drives commitment to hemogenic fate and HSC productionduring vertebrate development remain largely unexplored. (grantome.com)
Notch receptors and Notch ligands were detected in virtually all cells throughout EB development. (karger.com)
During embryonic development, the unique cellular phenotypes of the adult are established. (edu.au)
We have shown that MOZ/MYST3 is essential for the development of haematopoietic stem cells and that QKF/MYST4 has a critical role in neural stem cells and in brain development. (edu.au)
We are currently investigating the function of chromatin modifiers during embryonic development, in adult stem cell populations and in cancer, and modelling chromatin-based intellectual disability to discover ameliorating treatments. (edu.au)
The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. (thermofisher.com)
These cells can be used in future studies for the development of novel treatment options and to study the pathophysiology of GS-2 disease. (biomedcentral.com)
The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
Vessel development involves generating primary vascular endothelial tubes by aggregation of angioblasts (vasculogenesis), creating a vascular network through endothelial sprouting (angiogenesis), and pruning primary vascular tubes by recruiting smooth muscle cells to the vessel walls (vessel maturation). (chinagene.cn)
Although several factors such as vegf, flk1, cloche, lycat and estrp are essential for angioblast development, much of the signaling pathways underlying the derivation of angioblasts from the hemangioblasts or Flk1+ mesodermal cells remain unknown. (chinagene.cn)
Vascular endothelial cell development and underlying mechanisms[J]. HEREDITAS, 2012, 34(9): 1114-1122. (chinagene.cn)
Notch signaling is required for arterial-venous differentiation during embryonic vascular development. (chinagene.cn)
Two molecules related to the VEGF receptor are expressed in early endothelial cells during avian embryonic development. (chinagene.cn)
This indicates that Rpl22 and Rpl22l1 play distinct and essential roles in supporting B and T cell development. (bvsalud.org)
Although HECs are thought to be primarily limited to the aorta-gonad-mesonephros (AGM) during early development, EHT has been described in various other hematopoietic organs and embryonic vessels. (bu.edu)
In the earliest stages of development, the physical properties of the microenvironment can direct cell differentiation, and initiate the coordinated movement of groups of cells to establish the patterns that will define how the body is arranged. (mechanobio.info)
Cardiac specification occurs very early on during embryonic development. (mechanobio.info)
For instance, in mouse embryos this occurs as early as day 6.5 post fertilization and involves the development of a population of cardiac precursor cells in the lateral posterior epiblast just prior to formation of the primitive streak - a structure which marks the beginning of gastrulation. (mechanobio.info)
During development, the nuclear translocation of β-catenin, a transcriptional cofactor in the Wnt signaling pathway, is controlled by the extent of cell-cell contacts formed between cells. (mechanobio.info)
however, it has been shown to play a role in the regulation of embryonic and ocular development. (cancerindex.org)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Eric L. Golliher, NASA Goddard Space Flight CenterThe Thermophysics TC does looking Technical areas on degrees fascinated to all companies of trigeminal transport and ectoderm development and their aerodynamic groove Berlins for technology and columnar P. These vascular partners are labelled to stage into embryonic dialog embryos by the ,000 Written by AIAA. (need4speed.com)

The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)
Malignant GEP-NET cells expressed genes and regulons associated with normal, gastrointestinal endocrine cell differentiation, and fate determination stages. (bu.edu)
However, infiltrating myeloid cell types within both primary and metastatic GEP-NETs were enriched for genes encoding other immune checkpoints, including VSIR (VISTA), HAVCR2 (TIM3), LGALS9 (Gal-9), and SIGLEC10. (bu.edu)

Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)

What is cardiomyocyte differentiation? (mechanobio.info)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)

In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research. (stanford.edu)

Here, we show that HSC-like cells were efficiently induced from mouse ESCs by enforced expression of Lhx2, a LIM-homeobox transcription factor. (ebiotrade.com)

The enhanced osteogenic differentiation of hDPSCs due to ETV2 overexpression was partially reversed by treatment with inhibitors of ERK/MAPK or PI3K-AKT signaling. (biomedcentral.com)
Inhibition of the p38 MARK pathway down-regulates the activity of protein kinase C ( PKC ), which plays an important role in osteogenic differentiation of cells. (cusabio.com)

Co-expression of GFP with the receptor tyrosine kinase Flk1 revealed the emergence of three distinct cell populations, GFP - Flk1 - , GFP + Flk1 - and GFP + Flk1 + cells, which represent a developmental progression ranging from pre-mesoderm to prehemangioblast mesoderm to the hemangioblast. (elsevierpure.com)
Widespread Notch1 activation was evident at days 2-4 of EB differentiation, the time window of hemangioblast generation in this in vitro system. (karger.com)

These data define a unique phenotype for adult kidney-derived cells, which have potential as stem cells and may contribute to the regeneration of injured kidneys. (tau.ac.il)
Due to their capability to transdifferentiate, to immune modulate and to increase neuroplasticity by producing neurotrophic factors, adult stem cells (ASC) might fill this gap. (biomedcentral.com)
One explanation for deficient repair is when undifferentiated adult stem repair cells are inadequate in number or cannot be stimulated within the damaged tissue site. (journalofprolotherapy.com)
Adult stem cells turn out to be a more robust and reliable source of cell to heal damaged cells. (drcremers.com)
These adult stem cells, such as from fat (adipose derived) or bone marrow, have the ability to transform into different cells. (drcremers.com)

In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)

Furthermore, cells achieve the proteomic stabilization through keeping the normal state of molecular chaperones, the protein clearance pathways and the stress-associated signaling networks [ 14 ]. (ijbs.com)

Cardiomyocytes are the chief cell type in the heart and their coordinated contraction as a mass is responsible for the pumping of blood around the developing embryo. (mechanobio.info)

These results demonstrate that ectopic expression of Lhx2 confers an in vivo engrafting capacity to ESC/iPSC-derived hematopoietic cells and in vivo behavior of iPSC-derived hematopoietic cells is almost identical to that of ESC-derived cells. (ebiotrade.com)
Ex vivo HSC expansion has been suggested as an approach to improve hematopoietic reconstitution from low-cell dose grafts. (stanford.edu)
Furthermore, we provide evidence that HSC-selective ex vivo cultures deplete GvHD-causing T cells and that this approach can be combined with genotoxic-free antibody-based conditioning HSCT approaches. (stanford.edu)
In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
Un rôle majeur des prostaglandines dans l'hématopoïèse, ainsi mis en évidence, a pu être étendu à la souris chez qui un traitement par la prostaglandine PGE2 provoque la multiplication des CSH in vivo et in vitro, un résultat qui génerera très probablement des applications cliniques. (hypotheses.org)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

This review will summarize our current knowledge, challenge, and future directions on molecular and cellular mechanisms of endothelial cell develop-ment. (chinagene.cn)

Further, premature blood cell differentiation when PSC specification or signaling was impaired, led to assigning the PSC a role equivalent to the vertebrate hematopoietic niche. (sdbonline.org)

JNK can change the mRNA level of osteocalcin , so the activation of JNK can induce osteoblast differentiation. (cusabio.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)

Blood cell production in the Drosophila hematopoietic organ, the lymph gland , is controlled by intrinsic factors and extrinsic signals. (sdbonline.org)

Taken together, our data defined Flk2- and IL7Rα-positive TLC differentiation paths, and revealed functional roles of Flk2 and IL7Rα in TLC establishment. (bvsalud.org)
Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)

Specifically, Tie2-Cre-mediated ablation of Rpl22l1 in hemogenic endothelium impairs the emergence of embryonic hematopoietic stem cells. (bvsalud.org)
Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium. (ox.ac.uk)

1, 2 Recently, Prolotherapists have begun to utilize the potential of autologous adipose (fat)-derived stem/stromal cells (AD-SC) within non-manipulated fat graft scaffolding, combined with high-density PRP concentrates (HD-PRP) to provide a potent biological therapeutic combination. (journalofprolotherapy.com)
Therefore we have chosen to use the term "adipose-derived stem/stromal cells" (AD-SC's), rather than simply "mesenchymal stem cells. (journalofprolotherapy.com)

Tissue regeneration approach using stem cells in different clinical settings is a medical sensation now-a-days. (samploon.com)
Human endometrial tissue composed of epithelial stem cells (0.22%), mesenchymal stem cells (1.25%) and side population stem cells. (samploon.com)
Explaining the difference between embryonic and cord tissue-derived stem cells. (tomeipowered.com)
Tissue-resident lymphoid cells (TLCs) span the spectrum of innate-to-adaptive immune function. (bvsalud.org)
In order to reach these needs, recently, experiments with cell based therapies to rescue or replace dopamine-secreting cells, or with cells able to secrete paracrine factors modulating brain tissue repair were initiated [ 8 - 12 ]. (biomedcentral.com)
Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
With improved understanding of tissue healing and regeneration, stem cell Prolotherapy is gaining significant clinical importance and potential. (journalofprolotherapy.com)
Stem cells are known to work via a variety of methods to heal tissue depending on where the stem cells come from and into which tissue it is being placed. (drcremers.com)
Embryonic stem cells have been fraught with difficulties in coaxing them into "good/normal" tissue and there are major ethical issues. (drcremers.com)

Here, we investigated the single-cell transcriptomes of tumor and immune cells from patients with gastroenteropancreatic NETs. (bu.edu)
Initial analysis of Collier/Early B Cell Factor function in the lymph gland revealed the role of the Posterior Signaling Center (PSC) in mounting a dedicated cellular immune response to wasp parasitism. (sdbonline.org)
M cell maturation and cDC activation determine the onset of adaptive immune priming in the neonatal Peyer's patch. (costalab.org)

This endothelial-to-haematopoietic transition (EHT) involves the bending out and rounding up of endothelial cells and transition into haematopoietic cell clusters that protrude into the lumen of the arteries. (ox.ac.uk)
For example, humans are derived from fertilized eggs, and this process involves the differentiation of embryonic stem cells. (cusabio.com)

Leukemia inhibitory factor (LIF), Hepatocyte growth factor (HGF) and stem-cell factor (SCF) have the essential contribution in endometrial regeneration. (samploon.com)

In addition, these cells are enriched for β1-integrin, are cytokeratin negative, and show minimal expression of surface markers that typically are found on bone marrow-derived mesenchymal stem cells. (tau.ac.il)

Explant cultures of murine and human fetal lungs display adherent endothelial cells transitioning into floating hematopoietic cells, accompanied by the gradual loss of an endothelial signature. (bu.edu)

Finally, the developmental arrest of T cells was not rescued by elimination of the Rpl22l1 paralog, Rpl22, as we had previously found for the emergence of hematopoietic stem cells. (bvsalud.org)

During gastrulation, as the epiblast cells migrate through the primitive streak, they undergo epithelial-mesenchymal transition and form the mesoderm. (mechanobio.info)

The KSL cell induction frequency was superior to the case of Hoxb4. (ebiotrade.com)

In the early stage of adipocyte differentiation, ERK1/2 promotes adipocyte differentiation by promoting the expression of C/EBPα and PPARγ. (cusabio.com)

Activation of the Aplnr pathway in this system impaired the generation of long-term reconstituting HSPCs and appeared to drive myeloid differentiation. (ed.ac.uk)

To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro . (drcremers.com)

We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)

In vitro, they are plastic adherent and slowly proliferating and result in inhibition of alloreactive CD8 + T cells, indicative of an immuneprivileged behavior. (tau.ac.il)
A higher proportion of CD34 + cells (∼ 20%) could be derived from hPSCs by inhibition of mitogen-activated protein kinase (MAPK) extracellular signal-regulated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling and activation of bone morphogenic protein-4 (BMP4) signaling. (ashpublications.org)

FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)

It may start with a range of non-motor symptoms such as disorders of the autonomic nervous system, olfaction, sleep, mood and subtle cognitive deterioration, before a degeneration of the dopamine producing cells in the upper brainstem (nigral substance) may manifest with motor parkinsonism, the clinical hallmark of this disease, and way before involvement of the neocortex induces dementia [ 5 ]. (biomedcentral.com)
The majority of the cited preclinical and clinical studies use expanded and/or induced mesenchymal stem cells. (biomedcentral.com)
Tumor and lymphoid compartments sparsely expressed immunosuppressive targets commonly investigated in clinical trials, such as the programmed cell death protein-1/programmed death ligand-1 axis. (bu.edu)
The standard method to culture LSCs on 3T3 feeder cells that have been used in clinical study is cultivating single LSC directly on top of the growth-arrested 3T3 feeder cells [ 14 ]. (drcremers.com)

The cytoskeleton is a highly dynamic network of filamentous proteins that enables the active transport of cellular cargo, transduces force, and when assembled into higher-order structures, forms the basis for motile cellular structures that promote cell movement. (mechanobio.info)

We compared the expression and distribution of ALDH1A2 in the different segments, anatomic regions and neural cells of spinal cord at the different stages of WT and Tg(SOD1*G93A)1Gur mice applied the methods of fluorescent immunohistochemistry and western blot. (ijbs.com)

In the late stage of adipocyte differentiation, activated ERK1/2 phosphorylates PPARγ to inactivate it and inhibit adipocyte differentiation. (cusabio.com)
JNK signaling pathway also plays an important role in regulating adipocyte differentiation. (cusabio.com)
In addition, the ERK5/BMK1 pathway, an atypical MAPK pathway, is involved in the regulation of adipocyte differentiation and fat metabolism. (cusabio.com)

We study the embryonic origins of blood stem cells with the aim to inform the generation of these cells in culture, and ultimate produce clinically relevant blood stem cells for therapeutic purposes. (ox.ac.uk)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)

Anatomy57

Organisms22

Diseases12

Chemicals and Drugs57

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes60

Disciplines and Occupations3

Information Science3

Named Groups1

Health Care2

Progenitor26

Progenitors12

Differentiate14

Lineages9

Vitro differentiation2

HSCs12

Precursors5

Pluripotent9

Lineage8

MSCs21

Epithelial cells2

Self-renewal2

Hematopoiesis2

Tissues6

Transplantation5

Gene12

Multipotent2

Runx16

Proliferation7

Development25

Genes3

Germ1

Cardiomyocyte differentiation2

IPSCs1

ESCs1

Osteogenic differentiation2

Hemangioblast2

Adult5

Transcriptional1

Pathways1

Embryo1

Vivo6

Endothelial cell1

Specification1

Induce3

Organ1

Functional2

Endothelium2

Stromal Cells2

Tissue9

Immune3

Involves2

Regeneration1

Bone marrow-derived1

Murine1

Emergence1

Mesoderm1

Induction1

Promotes1

Myeloid1

Adipose-derived stem1

Activity is regulated1

Inhibition2

Transition1

Clinical4

Proteins1

Neural cells1

Adipocyte3

Therapeutic purposes2