Mesenchyme from human embryonic stem cells mesenchyme differentiation of cells. Medical search. Frequent questions

Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
Therefore, EGF and FGF induce cell proliferation and differentiation in the developing mouse SMG through activation of these signaling cascades ( 11 - 15 ). (iiarjournals.org)
Local factors, including transcription factors such as SRY-box 9 protein (SOX9), Runt-related transcription factor 2 (RUNX2), and bone morphogenetic proteins (BMPs), along with signaling pathways such as the Wnt pathway, play critical roles in chondrocyte proliferation and differentiation. (pfmjournal.org)
During the second week, a rapid proliferation and differentiation of the trophoblasts occurs. (medscape.com)

Here we show that LIS1 is essential for the viability of mouse embryonic stem cells (mESCs), and it governs the physical properties of these cells. (bvsalud.org)
Suggest GDF11 functions as encephalic regionalizing factor in neural differentiated mouse embryonic stem cells. (cusabio.com)

2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
In early experiments, physical separation of mesenchymal and epithelial stroma from embryonic murine thymus resulted in defective thymus development when epithelium was cultured in vitro in the absence of mesenchyme ( 7 ). (frontiersin.org)
These findings were confirmed in later studies in which removal of mesenchyme from embryonic day 12 murine thymic lobes impaired thymus growth in vitro ( 8 ). (frontiersin.org)
We have used RT-PCR to screen pluripotent murine embryonic stem cells to identify receptor tyrosine kinases (RTKs) potentially involved in the determination or differentiation of cell lineages during early mouse development. (silverchair.com)
On day E10.5 of murine development, the metanephric mesenchyme secretes glial cell line-derived neurotrophic factor (GDNF). (j-organoid.org)

Embryo-like models with spatially organized morphogenesis and structure of all defining embryonic and extra-embryonic tissues of the post-implantation human conceptus (that is, the embryonic disc, the bilaminar disc, the yolk sac, the chorionic sac and the surrounding trophoblast layer) remain lacking1,2. (bvsalud.org)
Here we establish a model of the human post-implantation embryo, a human embryoid, comprising embryonic and extraembryonic tissues. (bvsalud.org)
With all its complexity, the human body emerges from a small cluster of cells that undergo division and morph into different types of tissues. (interestingengineering.com)
While the new human embryo-like models are not as advanced as their mouse counterparts and do not possess beating heart-like structures, they do contain both embryonic and extraembryonic tissues that would typically develop into the placenta, yolk sac, and amnionic sac. (interestingengineering.com)
Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
In order to address the limits associated with current therapies, many research groups have investigated various regenerative medicine strategies utilizing different stem cell sources to engineer artificial salivary tissues that can mitigate the effects of xerostomia and hyposalivation. (biomedcentral.com)
The meeting will feature sessions on cell signaling, clinical genetics, sense organs, development of animal models of craniofacial disorders, state-of-the-art imaging techniques, and the use of tissue engineering approaches for regenerative repair of craniofacial tissues. (grc.org)
MSCs make the different specialized cells found in the skeletal tissues. (tomeipowered.com)
While many studies have demonstrated the importance of cTEC and mTEC during T-cell development, thymic stromal microenvironments also contain heterogenous non-epithelial stromal (NES) populations in a similar manner to that seen in peripheral lymphoid tissues. (frontiersin.org)
The progressive generation of chick and mouse axial tissues - the spinal cord, skeleton and musculature of the body - has long been proposed to depend on the activity of multipotent stem cells. (silverchair.com)
Despite all the available protocols for successful cryopreservation of different cell types, cryopreservation of organs and tissues is still considered difficult. (iiarjournals.org)
The tissue source issue has been overcome with the introduction of stem cell lines, particularly induced pluripotential stem cells (iPSCs) that can be produced from adult tissues. (amegroups.org)
Octamer-binding protein 4 (Oct-4) is a molecular marker for stem cells that plays an essential role in maintaining pluripotency and self-renewal processes in various types of benign and malignant tissues. (biomedcentral.com)
Epithelial to mesenchymal transition (EMT) is a central mechanism for diversifying the cells found in complex tissues. (bioseek.eu)
This dynamic process helps organize the formation of the body plan, and while EMT is well studied in the context of embryonic development, it also plays a role in the genesis of fibroblasts during organ fibrosis in adult tissues. (bioseek.eu)
cells either divide asymmetrically to preserve stem cell progenitors, partition into sister cells, differentiate along fate pathways, or undergo oncogenesis following the formation of normal tissues. (bioseek.eu)
Once the wound is healed, the cells of the stump must mobilise under the wound epidermis and begin the process of replacing lost tissues, by forming a proliferating blastema. (biomedcentral.com)
Most connective and skeletal tissues of the cranium and face ultimately come from the derivatives of neural crest cells. (medscape.com)

Such human fully integrated and complete SEMs recapitulate the organization of nearly all known lineages and compartments of post-implantation human embryos, including the epiblast, the hypoblast, the extra-embryonic mesoderm and the trophoblast layer surrounding the latter compartments. (bvsalud.org)
These include embryonic disc and bilaminar disc formation, epiblast lumenogenesis, polarized amniogenesis, anterior-posterior symmetry breaking, primordial germ-cell specification, polarized yolk sac with visceral and parietal endoderm formation, extra-embryonic mesoderm expansion that defines a chorionic cavity and a connecting stalk, and a trophoblast-surrounding compartment demonstrating syncytium and lacunae formation. (bvsalud.org)
The BMP, Wnt and Nodal signaling requirements for the differentiation of specific mesoderm subtypes in mammals have been mapped in detail, but how FGF shapes mesodermal cell type diversity is not precisely known. (biologists.com)
It is also not clear how FGF signaling integrates with the activity of other signaling systems involved in mesoderm differentiation. (biologists.com)
Pluripotent stem cells, including embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny representative of all three germ layers (ectoderm, endoderm, and mesoderm). (sigmaaldrich.com)
osteoblasts, chondrocytes, endothelial-cells and also non-mesoderm-type lineages e.g. (tomeipowered.com)
EMT is usually a fundamental process in normal embryonic development, particularly during formation of mesoderm, neural crest formation and heart valve development [10]. (bso14.org)
During the metanephric development process, epiblasts expressing T (brachyury) give rise to nascent mesoderm on embryonic day (E) 7.5. (j-organoid.org)

Androgen induced expression of androgen receptor (AR), and co-culture with rat urogenital sinus further induced expression of prostate-specific antigen, a hallmark of secretory cells, suggesting that E-PZ-iPS-like cells have the capacity to differentiate into prostatic basal and secretory epithelial cells. (nih.gov)
During the development of multicellular organisms, precursor cells for specialized organs need to differentiate in reproducible proportions and in spatially coherent domains. (biologists.com)
Stem cells have the unique ability to self-renew or to differentiate into various cell types in response to appropriate signals. (sigmaaldrich.com)
Embryonic stem cells have the ability to differentiate into more cell types than adult stem cells. (sigmaaldrich.com)
ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes. (sigmaaldrich.com)
while the subchondral bone region enhances the environment for cells which migrate into that region's macrostructure and which differentiate into osteoblasts. (patsnap.com)
Mesenchymal stem cells (MSCs), or stromal stem cells, can differentiate into many different types of … In contrast, treatments that utilize MSCs from a patientâ s fat (adipose) sample have shown weak or unreliable responses. (tomeipowered.com)
Concomitantly, the ability of MSCs to differentiate and give rise to multiple cell types such as adipocytes, chondrocytes, and osteoblasts … These transplants vastly increase the bodyâ s natural healing abilities and have potent anti-inflammatory and immunosuppressive responses.Â. (tomeipowered.com)
Mesenchymal Stem Cells Instruction Manual Product Description Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are self-renewing multipotent cells that can differentiate into a wide variety of cell types. (tomeipowered.com)
The MSCs and osteoprogenitors residing along the OFs keep proliferating, subsequently differentiate into osteoblasts, and contribute to the new bone formation through intramembranous ossification [ 4 ] [ 5 ] , which happens with a direct differentiation into osteoblasts from MSCs and/or osteogenic precursors without assuming a chondrogenic fate. (encyclopedia.pub)
6. Transcription factors - proteins that regulate which genes are transcribed in a cell - appear to be essential to determining the pathway particular stem cells take as they differentiate. (catsboard.com)
Meanwhile, the ureteric bud secretes Wnt-9b and fibroblast growth factor 9 (FGF9), which differentiate the metanephric mesenchyme into pretubular aggregates. (j-organoid.org)
These neuroectodermal crest cells are believed to migrate widely throughout the developing embryo in a relatively cell-free enriched extracellular matrix and differentiate into a wide array of cell and tissue types, influenced by the local environment. (medscape.com)

No objective, even though noble in itself, such as a foreseeable advantage to science, to other human beings or to society, can in any way justify experimentation on living human embryos or foetuses, whether viable or not, either inside or outside the mother's womb. (archdiocese-no.org)
To create embryos with the intention of destroying them, even with the intention of helping the sick, is completely incompatible with human dignity, because it makes the existence of a human being at the embryonic stage nothing more than a means to be used and destroyed. (archdiocese-no.org)
To overcome these limitations, scientists turned to laboratory models that emulate embryos using stem cells from both mice and humans, rather than relying on eggs and sperm. (interestingengineering.com)
These embryo-like models are not living entities capable of developing into fully formed embryos, but they do provide valuable insights into the intricate mechanisms of embryonic development, human defects and diseases, pregnancy failures, and even the potential for growing synthetic organs for transplantation. (interestingengineering.com)
A study by López-Márquez et al, utilizing mice, indicated that the Sox9 transcription factor is "likely at the center of an important regulatory network of signals that govern thyroid differentiation both in embryos and in adults. (medscape.com)
To directly test this model of Shh function, we have restored cell survival in Shh mutant embryos after transient Shh expression by concomitant removal of the pro-apoptotic genes Bak/Bax to intercept cell death, in order to rescue the proposed late Shh role. (nih.gov)

E-PZ-iPS-like cells demonstrated pluripotency by differentiating into ectodermal, mesodermal, and endodermal cells in vitro, although lack of teratoma formation in vivo and incomplete demethylation of pluripotency genes suggested only partial reprogramming. (nih.gov)
Gey developed new techniques for in vitro, or laboratory-based, maintenance of organs and hormonal tissue, created new methods for cell cultivation, and researched nutritional media, or cell food. (asu.edu)
Differentiation is triggered by various factors in vivo , some of which can be replicated in in vitro stem cell cultures. (sigmaaldrich.com)
These protocols, such as organoid cell culture methods, have provided more predictive in vitro cellular "Disease-in-a-Dish" models. (sigmaaldrich.com)
The ability to expand pluripotent cells in vitro and subject them to direct differentiation to produce specific cell types is crucial to the development of cell-based therapies to replace or restore tissue that has been damaged by disease or injury. (sigmaaldrich.com)
As a result of over five decades of investigation, mesenchymal stromal/stem cells (MSCs) have emerged as a versatile and frequently utilized cell source in the fields of … MSC have been shown to differ-entiate in vitro into adipocytes, chon-drocytes, osteoblasts, myocytes, and ß-pancreatic islets cells. (tomeipowered.com)
The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. (tomeipowered.com)
Cryopreservation of cell lines has been widely used in the laboratory for in vitro study of cell systems. (iiarjournals.org)
Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. (nature.com)
These findings suggest that a lethal lung disease can be targeted and corrected in a human lung organoid model in vitro . (nature.com)
Gene therapy has been utilized successfully to repair or inactivate mutations in animal models of monogenic human diseases 19 as well as human in vitro cells 12 . (nature.com)

Model built using human stem cells offers a unique opportunity to study early embryonic development. (interestingengineering.com)
Led by renowned biologist Magdalena Zernicka-Goetz, and Bren Professor of Biology and Biological Engineering at Caltech, the team generated the model using human stem cells, offering a unique opportunity to study the intricate processes occurring during early embryonic development. (interestingengineering.com)
In early embryonic mammary gland development, Isatoribine the formation of the mammary epithelial placodes in the skin is a critical event. (bso14.org)

IMs are found in the space between the tissue stroma cells and contain, among other components, fibrillar collagens and fibronectin. (nature.com)
Broadly separated into mesenchymal and endothelial cells, NES have been implicated in thymus organogenesis, thymocyte development, tolerance induction and development/maintenance of epithelial stroma. (frontiersin.org)
In this review, we cover how the non-epithelial compartment of thymic stroma represent essential cell populations in regulating thymus function. (frontiersin.org)
The branching nature of the epithelial ducts in the breast requires a level of phenotypic plasticity enabling cells to invade the underlying stroma. (bso14.org)
The cells can achieve this using unique Isatoribine mechanisms including collective migration [7, 8] or epithelial to mesenchymal transition (EMT) where leading cells at the tip of the branching structures acquire mesenchymal characteristics that facilitate migration into the surrounding stroma [9]. (bso14.org)
this gives rise to the cap mesenchyme and interstitial stroma populations ( Fig. 1 ) [ 4 - 6 ]. (j-organoid.org)

In addition to providing biomechanical support to the islets, ECM components are required for β-cell development, proliferation, survival, and proper insulin secretion. (nature.com)
Help us to be faithful to this sacred trust so that we may protect and promote the dignity of every human life from the very moment of conception, particularly the tiniest humans in the embryonic stage of human development who are so often forgotten by society & used by modern science. (archdiocese-no.org)
Although vitamin A is necessary for fetal development, most women carry stores of vitamin A in their fat cells, so over-supplementation should be strictly avoided. (wikipedia.org)
The ability to study human post-implantation development remains limited owing to ethical and technical challenges associated with intrauterine development after implantation1. (bvsalud.org)
This SEM platform will probably enable the experimental investigation of previously inaccessible windows of human early post implantation up to peri-gastrulation development. (bvsalud.org)
In conclusion, we present a modular, tractable, integrated3 model of the human embryo that will enable us to probe key questions of human post-implantation development, a critical window during which substantial numbers of pregnancies fail. (bvsalud.org)
Scientists have successfully created an embryo-like model replicating the crucial post-implantation stage of human development. (interestingengineering.com)
Zernicka-Goetz and her team have now taken another step forward by developing a human embryo-like model that simulates the second week of human development-after the embryo has successfully implanted in the womb. (interestingengineering.com)
The creation of this human embryo-like model represents a significant milestone in our quest to comprehend the complex processes underlying early human development. (interestingengineering.com)
The general underlying strategy behind the development of tissue engineered organ substitutes is the utilization of a combination of cells, biomaterials, and biochemical cues intended to recreate the natural organ environment. (biomedcentral.com)
This review considers the genetic and epigenetic control of nephrogenesis, together with the epigenetic mechanisms that accompany kidney development and recent advances in induced reprogramming and kidney cell regeneration in the context of DN. (emjreviews.com)
The bone cells do many things for the skeletal system such as the development of new bones, the maintenance of bones, and the regulation of minerals in the body. (patsnap.com)
The conversion of an epithelial cell to a mesenchymal cell is critical to metazoan embryogenesis and a defining structural feature of organ development. (rupress.org)
Throughout the growth and development of the skull, the cranial suture mesenchyme remains unossified, which interposes between the OFs of the adjoining bone plates. (encyclopedia.pub)
The thymus supports T-cell development via specialized microenvironments that ensure a diverse, functional and self-tolerant T-cell population. (frontiersin.org)
Extensive research on thymic epithelial cells (TEC) within the cortex and medulla has defined their essential roles during T-cell development. (frontiersin.org)
However, experimental evidence suggests these cells are important for thymus function by either directly influencing T-cell development, or by indirectly regulating TEC development and/or function. (frontiersin.org)
While adult lymphoid progenitor cells are produced by the bone marrow, their lineage commitment and development into mature T-cells is dependent on their migration into the thymus, where essential interactions with heterogeneous thymic stromal cells take place ( 1 ). (frontiersin.org)
In the process of tooth development, complex orchestration between genetic and epigenetic programs regulates the spatiotemporal expression of cell proliferation-, differentiation-, and migration-related genes, and finally tooth formation. (gjkqyxzz.cn)
We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
Hh proteins are secreted morphogens that play essential roles in regulation of embryogenesis, development, tissue homeostasis, regeneration and stem cell maintenance in a concentration-dependent manner [ 6 ]. (biomedcentral.com)
Mouse SMG rudiment development begins on embryonic day 12 (E12) when the epithelial tissue of the floor of the mouth sinks towards the direction of the neck. (iiarjournals.org)
Although many differentiation protocols in the literature have been successful in mimicking lung development from stem cells, there has not been an examination of how a specific mutation impacts the differentiation process including its effects on the early endoderm, as well as the proximal and distal lung epithelial cell populations in the lung organoids. (nature.com)
Mechanical forces are critical for embryonic development - yet very little is known about their roles. (royalsociety.org)
The meeting focused on the role of mechanobiology in multiple aspects of embryonic development. (royalsociety.org)
Limb development is a particularly attractive model for unraveling the function and interaction of such regulatory components, because it is very accessible to study and is extremely well conserved between genetic (mouse) and embryologic (chick) model organisms and humans. (nih.gov)
Recently, we have focused on the role of Shh/Gli3 and 5'Hoxd genes in regulating several aspects of limb development, including early digit patterning, as well as condensation and differentiation of cartilage precursors during final morphogenesis of the limb skeleton. (nih.gov)
Thus, understanding how normal transcription programs are regulated during development may also help to decipher abnormal gene expression patterns in tumors and devise new strategies to intercept cellular targets driving tumor cell behavior. (nih.gov)
Increased understanding of branching morphogenesis in the breast gland and the regulation of EMT and Isatoribine its reverse process mesenchymal Isatoribine to epithelial transition (MET) may hold the keys for future development of methods/drugs that neutralize the invading properties of malignancy cells. (bso14.org)
The development of animals is dominated by cell-cell interactions and by differential gene expression. (catsboard.com)
7. Cell Memory Underlies Cell Decision-Making Underlying the richness and astonishingly complex outcomes of development is cell memory. (catsboard.com)
Many histone modifiers and chromatin remodelers have been implicated in stem cell pluripotency, cellular differentiation and development. (catsboard.com)
10. The Sugar Code forms information-rich structures that influence the arrangement of different cell types during embryological development. (catsboard.com)
Beauty and the development finds embryonic-extraembryonic as it is this middle, with Villeneuve's stage Completing completed as a Aircraft PMA for enthusiasts and Beaumont ruptures seeing obligated as a entire contribution for posts. (need4speed.com)
Gene Ontology analysis showed that genes involved in embryonic development and growth are significantly over-represented in regenerating early hindlimb buds and that successful regeneration in the Xenopus hindlimb correlates with the induction of stress response pathways. (biomedcentral.com)
By the end of week 2, the development of primary chorionic villi is observed, the primary yolk sac is replaced by the secondary yolk sac, and the amniotic cavity appears as a space between the cytotrophoblasts and the inner cell mass. (medscape.com)

The human embryo undergoes morphogenetic transformations following implantation into the uterus, but our knowledge of this crucial stage is limited by the inability to observe the embryo in vivo. (bvsalud.org)
The caudal segment of the thyroglossal duct undergoes histologic differentiation into follicular elements at gestational weeks 10 and 11. (medscape.com)
The breast gland undergoes repeated cycles of cell proliferation, differentiation and involution from menarche to menopause at which point hormonal signals, or lack thereof, cause cell death by triggering a combination of apoptosis and senescence [4C6]. (bso14.org)
The process reaches its limit when a cell undergoes terminal differentiation to form one of the highly specialized cell types of the adult body. (catsboard.com)
During mitosis, cells undergo symmetrical cell division, while oocyte meiotic maturation undergoes two consecutive, asymmetric divisions that generate a totipotent haploid oocyte and two small polar bodies not involved in DNA replication. (bioone.org)
The ciliary action of the uterine tube transports the zygote to the uterus, during which it undergoes a series of mitotic divisions and transforms into the ball of small cells termed blastomeres. (medscape.com)

In contrast, culturing on Laminin-421 induced the expression of β-cell genes, including Ins1 , MafA , and Glut2 , and significantly improved glucose-stimulated insulin secretion. (nature.com)
These E-PZ-iPS-like cells with normal karyotype gained expression of pluripotent genes typical of iPS cells (Tra-1-81, SSEA-3, Nanog, Sox2, and Oct4) and lost gene expression characteristic of basal prostatic epithelial cells (CK5, CK14, and p63). (nih.gov)
In an adult organism, the genes on the Y-chromosome help produce the male gamete, the sperm cell. (asu.edu)
This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
A major reason for the focus on therapies for RP has been that excellent mouse models of the disease exist, including mutations in many of the genes that cause the human disease. (amegroups.org)
My lab previously identified several transcription factors (homeobox genes and T-box genes) involved in formation and patterning of both the primary embryonic axis and the limb axis in vertebrates. (nih.gov)
hundreds of human genes encode signal proteins, cell-surface receptors, cell adhesion proteins, or ion channels that are either not present in yeast or present in much smaller numbers. (catsboard.com)
more than 1000 human genes encode transcription regulators, but only about 250 yeast genes do so. (catsboard.com)
Both the genes a cell expresses and the way it behaves depend on the cell's past, as well as on its present circumstances. (catsboard.com)

Models of the embryo derived from stem cells are important tools for interrogating developmental events and tissue-tissue crosstalk during these stages1. (bvsalud.org)
Modulation of the subpopulations in the hypoblast-like compartment demonstrates that extraembryonic-like cells influence epiblast-like domain differentiation, highlighting functional tissue-tissue crosstalk. (bvsalud.org)
Stem cell-derived models of the embryo are important tools to interrogate developmental events and tissue-tissue crosstalk during these stages1. (interestingengineering.com)
Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
The purpose of this review is to highlight current bioengineering approaches for salivary gland tissue engineering and the adult stem cell sources used for this purpose. (biomedcentral.com)
This article is intended to provide a review of the most current approaches to utilizing stem cells and bioengineering principles for the purpose of salivary tissue regeneration. (biomedcentral.com)
These properties provide stem cells with powerful capabilities for tissue repair, replacement, and regeneration, so human stem cells are of special interest in medical research. (sigmaaldrich.com)
The Growth Differentiation Factor 11 (GDF11) and Myostatin (MSTN) in tissue specific aging. (cusabio.com)
Bone cell' Bone cells', which are found within the bone tissue, are responsible for the make-up of the skeleton of vertebrates. (patsnap.com)
They are made up of different bone cells: there is the compact tissue which is the hard outer part of the bone, the cancellous tissue which is all the spongy tissue inside of the compact tissue, and the subchondral tissue which is the smooth tissue at the end of each bone. (patsnap.com)
The connective tissue capsule and the interlobar septa develop from the fourth pharyngeal pouch's neural crest mesenchyme. (medscape.com)
Mesenchymal stem cells (MSCs) are an example of tissue or 'adult' stem cells. (tomeipowered.com)
Explaining the difference between embryonic and cord tissue-derived stem cells. (tomeipowered.com)
The postcranial axis (i.e. tissue caudal to the head) is then generated over an extended period in a rostral-to-caudal sequence by cells that are derived from the primitive streak and the adjacent epiblast cells, which together eventually form the tail bud. (silverchair.com)
Fluorescent staining of actin filaments in fixed tissue sections and tissue culture cells preparations.Note: Unlike many actin antibodies, Acti-stain™ 555 phalloidin binds only to F-actin resulting in low background fluorescence. (cytoskeleton.com)
Phalloidin has been labeled with a proprietary red fluorescent dye which allows it to be used to stain actin filaments in tissue cultured cells and tissue sections (1, see Figure 1) and cell-free preparations. (cytoskeleton.com)
While cells could survive as grafts for several months, there was little evidence for true integration into host tissue and immature photoreceptors did not mature into fully functional structures ( 5 , 6 ). (amegroups.org)
In addition, while useful for mechanistic studies, the work in mice has clearly not been applicable to human since no equivalent source of tissue is available. (amegroups.org)
Although endometriosis is known as a benign disease, it has demonstrated characteristics of malignant neoplasms, including neoangiogenesis, tissue invasion, and cell implantation to distant organs. (biomedcentral.com)
Retrograde menstruation is the most widely hypothesized cause for the implantation of endometrial tissue in the peritoneal cavity and the survival of endometrial cells [ 10 ]. (biomedcentral.com)
Due to the immediate exposure of epithelial tissue to external environment cellular remodeling and renewal occurs relatively fast, meaning that new cells are constantly replacing older cells. (bso14.org)
One of the crucial questions is cell fate determination and differentiation, tissue formation, and what, what cell phenotype type each cell will become. (catsboard.com)
While all vertebrates are capable of some types of tissue regeneration, most, including humans, have lost the ability to regenerate whole structures such as limbs ( epimorphic regeneration ), [ 1 ]. (biomedcentral.com)

While some islet BM components are produced by endothelial cells (ECs), the source of others remains unknown. (nature.com)
2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
Significantly, there are additional non-epithelial stromal cells (NES) that exist alongside TEC within thymic microenvironments, including multiple subsets of mesenchymal and endothelial cells. (frontiersin.org)

Beginning in the 1980s, many studies of human populations used the Y-chromosome gene sequences to trace paternal lineages. (asu.edu)
Here, we address these questions by analyzing the effects of targeted signaling manipulations in differentiating stem cell populations at single-cell resolution. (biologists.com)
Although the main cell-cell communication systems that drive and coordinate differentiation in cell populations have been identified, how these systems interact in signaling networks is still not well understood. (biologists.com)
Using such approaches, researchers can address how craniofacial cell populations work together, and how the surrounding environment impacts on form. (grc.org)
Arguably, the most recognized and well-studied stromal populations in thymus biology are thymic epithelial cells (TEC) within the cortical and medullary areas, which are defined by their anatomical separation and function. (frontiersin.org)
Cortical epithelial cells (cTEC) support the earliest thymocyte progenitor populations, guiding them through the cortex, directing them to a T-cell lineage fate and ensuring their functional qualities as self-MHC restricted cells through the process of positive selection ( 2 ). (frontiersin.org)
Here we show the differentiation of patient specific iPSCs derived from a patient with SFTPB deficiency into lung organoids with mesenchymal and epithelial cell populations from both the proximal and distal portions of the human lung. (nature.com)

These human complete SEMs demonstrated developmental growth dynamics that resemble key hallmarks of post-implantation stage embryogenesis up to 13-14 days after fertilization (Carnegie stage 6a). (bvsalud.org)
We offer a large collection of cell culture media, supplements, bioactive small molecules, and growth factors used to control the cell fate of human iPSCs. (sigmaaldrich.com)
The research report titled Global Mesenchymal Stem Cells Market Growth (Status and Outlook) 2020-2025 released by MarketsandResearch.biz covers industry segment by applications, by type, regional outlook, market demand, latest trends, and industry share & revenue by manufacturers, company profiles, and growth forecasts - 2025. (tomeipowered.com)
Making a tooth: growth factors, transcription factors, and stem cells [J]. Cell Res, 2005, 15(5):301-316. (gjkqyxzz.cn)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
The epithelial cells constituting SMG rudiments promote BrM when stimulated with cell growth factors. (iiarjournals.org)
EGF and FGF are secreted mainly from the mesenchyme, and growth factor receptors are expressed in the epithelial cells of SMG rudiments ( 12 , 13 ). (iiarjournals.org)
Growth, metabolism, and differentiation of chondrocytes, which are the key cellular players in this process, are regulated by systemic hormones, local factors, and cellular signaling pathways. (pfmjournal.org)
Chondrocyte growth, metabolism, and differentiation are complex processes. (pfmjournal.org)
The resting zone, a source of stemlike progenitor cells that restores the reservoir of proliferative chondrocytes, is located farthest from the primary ossification center and assumes responsibility for preserving the architectural integrity of the growth plate [ 4 ]. (pfmjournal.org)
These results suggested dual Shh roles that can be uncoupled: a transient role in directing cell fate and morphogenesis, and a sustained role in promoting growth. (nih.gov)
Evidence is gradually accumulating which tends to show that the reduction of the number of chromosomes may take place during the last divisions of the germ mother cell, that is before the growth of the oocyte or spermatocyte I commences, and therefore before maturation commences. (co.ma)

Combining our CRISPR, ZFN gene editing , and stem cell expertise, we now offer novel stem cell lines, optimized media, and innovative kits for all areas of stem cell biology, including induced pluripotent stem cells (iPSCs), neural, mesenchymal and hematopoietic stem cell culture. (sigmaaldrich.com)

3] Jussila M, Thesleff I. Signaling networks regulating tooth organogenesis and regeneration, and the specification of dental mesenchymal and epithelial cell lineages[J]. Cold Spring Harb Perspect Biol, 2012, 4(4):a008425. (gjkqyxzz.cn)

Mouse naive embryonic stem cells have recently been shown to give rise to embryonic and extra-embryonic stem cells capable of self-assembling into post-gastrulation structured stem-cell-based embryo models with spatially organized morphogenesis (called SEMs)3. (bvsalud.org)
2] Li Z, Yu M, Tian W. An inductive signalling network regulates mammalian tooth morphogenesis with implications for tooth regeneration[J]. Cell Prolif, 2013, 46(5):501-508. (gjkqyxzz.cn)
The group aims to understand the mechanisms of morphogenesis and cell differentiation within an embryo. (royalsociety.org)
Sonic hedgehog (Shh) signaling plays key roles in stem cell maintenance, developmental morphogenesis, and cancer pathogenesis. (nih.gov)
In this review we will focus on the cellular and molecular mechanisms of breast morphogenesis and EMT and its reversed process mesenchymal to epithelial transition (MET) and how these processes can be recapitulated in stromal-rich three-dimensional cell culture assays. (bso14.org)

Each arch consists of 3 layers: an outer covering of ectoderm, an inner covering of endoderm, and a middle core of mesenchyme. (medscape.com)

Adipose stem cells in reparative goat mastitis mammary gland. (sciendo.com)

Importantly, E-PZ-iPS-like cells re-expressed basal epithelial cell markers (CD44, p63, MAO-A) in response to prostate-specific medium in spheroid culture. (nih.gov)
Finally, when injected into mice, E-PZ-iPS-like cells expressed basal epithelial cell markers including CD44 and p63. (nih.gov)
E-cadherin is an epithelial cell-cell adhesion molecule that plays important role in the modulation of cell polarization, cell migration, and cancer metastasis. (biomedcentral.com)
Cells need to transit from strong epithelial cell-cell binding to a more mobile state to facilitate migration. (bso14.org)

The inner cell mass further differentiates into a bilaminar embryonic disc. (medscape.com)

Unlike the well-established perivascular niche of SSCs in the long bone, stem cells of the cranial bone are generally located and confined within the cranial suture mesenchyme, subsequently defined as SuSCs. (encyclopedia.pub)
Similarly, as the major stem cell population of cranial bones, the physiological significance of SuSCs is undoubted and self-evident. (encyclopedia.pub)
The inner layer, the hypoblast, lies adjacent to the blastocyst cavity, and its localized thickening, the prochordal plate, becomes the future embryonic cranial region. (medscape.com)

Cryopreservation is also useful for germ cells in medical care, for organic long-term preservation of induced pluripotent stem cells (iPS cells), and in animal husbandry ( 1 - 4 ). (iiarjournals.org)

Kidney tubuloids derived from adult stem cells offer the advantage of long-term culture and expansion, but they include only tubular structures and lack glomerular components. (j-organoid.org)

Thus, we hypothesized that pericytes regulate β-cells through the production of BM components. (nature.com)
We further found that the pericytic laminin isoforms differentially regulate mouse β-cells. (nature.com)
We also review the location and molecular characteristics of these putative stem cells, along with their evolutionary conservation in vertebrates and the signalling mechanisms that regulate and arrest axis extension. (silverchair.com)
These factors regulate gene expression, cell differentiation, and extracellular matrix synthesis. (pfmjournal.org)
Osr1 -expressing cell clusters spatially regulate Six2 expression, which in turn induces the cap mesenchymal population. (j-organoid.org)

Another study in mice, by Johansson et al, indicated "that embryonic thyroid differentiation is an asynchronous process," one that spans "several developmental stages of thyroid organogenesis. (medscape.com)
Philippa Francis-West is a Professor in Developmental and Cell Biology at the Centre for Craniofacial and Regenerative Biology, King's College London. (royalsociety.org)

Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)
Mesenchymal stem/stromal cells (MSCs) are multipotent cells that are emerging as the most promising means of allogeneic cell therapy. (tomeipowered.com)
These microenvironments are classically defined as distinct cortex and medulla regions that each contain specialized subsets of stromal cells. (frontiersin.org)
In malignancy, the adoption of mesenchymal phenotype by the malignant cells allows stromal invasion and subsequent intravasation to blood- or lymphatic vessels, a route that is a prerequisite for metastasis. (bso14.org)
Presently, kidney organoids contain not only nephrons and ureteric buds but also stromal cells. (j-organoid.org)

Although there are cell types in the adult that retain some degree of pluripotency, their range of options is generally narrow. (catsboard.com)

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture. (sigmaaldrich.com)

Epigenetic modifications play pivotal roles in organogenesis by controlling gene expression during cell fate determination and reprogramming. (gjkqyxzz.cn)
The Hh pathway is an evolutionarily conserved system for regulating patterning and cell fate from Drosophila to humans. (biomedcentral.com)
Normally, Hh ligand secreted by the notochord induces the ventral cell fate specification in the entire neural tube. (biomedcentral.com)

J Cell Biol (2006) 172 (7): 973-981. (rupress.org)
A summarization of DNA methyaltion modification research [J]. Chin J Cell Biol, 2010, 32(2):189-192. (gjkqyxzz.cn)

In one particular embodiment, methods for enhancing cellular functions on a surface of a medical device implant are disclosed which generally comprise providing a medical device implant comprising a plurality of nanofibers (e.g., nanowires) thereon and exposing the medical device implant to cells such as osteoblasts. (patsnap.com)
Epithelial organs therefore contain stem cells that are responsible for the continuous cellular remodeling [1]. (bso14.org)

Furthermore, the underlying mesenchyme remains rounded and does not expand to form a cone shaped blastema, a normal feature of successful regeneration. (biomedcentral.com)
Epimorphic regeneration can be thought of as occurring in two phases: wound healing and cell proliferation. (biomedcentral.com)

The thyroid gland forms as a proliferation of endodermal epithelial cells on the median surface of the developing pharyngeal floor. (medscape.com)
After differentiating the mutant and corrected cells into lung organoids, we show expression of SFTPB mRNA during endodermal and organoid differentiation but the protein product only after organoid differentiation. (nature.com)

The remaining follicular cells develop into a corpus luteum that produces E and progesterone (P). In turn, P promotes more proliferation within the uterus and vagina, and cornification in the vagina. (biomedcentral.com)
The tubular duct later solidifies into a cord of cells that will form the follicular elements. (medscape.com)
Desmosomes, molecular complexes of adhesion proteins, connect the cells, keeping follicular contents confined within the lumen. (medscape.com)

Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)

DNA methylation profiling identified key pathways and regulators of prostatic differentiation including Wnt5a, BPM7, PTEN, and NKx3.1 using E-PZ-iPS-lke cells. (nih.gov)

The female reproductive tract organs are dynamic and require synchronization of movement and differentiation to guide ovulated oocytes, prepare for implantation and nurture a fetus to develop as an independent organism. (biomedcentral.com)

Our functional studies demonstrate that the epiblast-like domain robustly differentiates into amnion, extraembryonic mesenchyme and primordial germ cell-like cells in response to bone morphogenetic protein cues. (bvsalud.org)

Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
2013) prepared SMGs from immature epithelial and mesenchymal cells obtained from fetal mouse SMGs and transplanted them into mice whose salivary glands had been removed ( 16 ). (iiarjournals.org)

The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)

Also covered in detail was the basic cell biology of EMT and its role in cancer and fibrosis, as well as the identification of new markers to facilitate the observation of EMT in vivo. (rupress.org)

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization. (sigmaaldrich.com)

Since suboptimal media may change the differentiation potential of stem cells, it is vital to select the correct stem cell-validated media and reagents at the start of your research process. (sigmaaldrich.com)

Suture mesenchymal stem cells (SuSCs), a heterogeneous stem cell population, belong to mesenchymal stem cells (MSCs) or skeletal stem cells (SSCs), with the ability to self-renew and undergo multi-lineage differentiation. (encyclopedia.pub)
In addition to intrathymic selection, and during this medullary residency period, CD4 + and CD8 + single positive (CD4SP, CD8SP) thymocytes acquire the ability to proliferate in response to TCR stimulation, and undergo licensing for cytokine production, prior to exiting the thymus to join the peripheral T-cell pool as Recent Thymus Emigrants (RTE) ( 5 , 6 ). (frontiersin.org)

A number of publications have exhibited that tumor initiating cells, sometimes referred to as malignancy stem cells adapt an EMT phenotype that renders them more resistant to apoptosis and drug therapy. (bso14.org)

The rudimentary lateral thyroid develops from neural crest cells, while the median thyroid, which forms the bulk of the gland, arises from the primitive pharynx. (medscape.com)

1 Institute for Stem Cell Research and MRC Centre for Regenerative Medicine,University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3JQ,UK. (silverchair.com)

Mice homozygous for a knock-out allele exhibit embryonic lethality and decreased histone acetylation. (jax.org)
Mice homozygous for a conditional allele activated in neuronal stem cells exhibit decreased NSC proliferation and premature differentiation and decreased number of late-born neurons. (jax.org)

It includes the possible stem cell therapy mechanisms involved and outcomes recorded so far, the limitations of using these regenerative medicines, and the progressive improvement in stem cell therapy by adopting approaches like PiggyBac, Sleeping Beauty, and the Sendai virus. (biomedcentral.com)

In addition, the authors also review future strategies in DN treatment such as transcriptional reprogramming of mature adult kidney cells into uncommitted induced pluripotent stem cells for renal repair and therapeutics. (emjreviews.com)
In order to find more promising treatments to stimulate renal repair, stem cell-based technology has been proposed as a potentially therapeutic option. (biomedcentral.com)

We combine two types of extraembryonic-like cell generated by overexpression of transcription factors with wild-type embryonic stem cells and promote their self-organization into structures that mimic several aspects of the post-implantation human embryo. (bvsalud.org)
Her integrated models combine embryonic and extraembryonic stem cells, representing the cells that develop into supporting structures for the growing embryo, such as the placenta and yolk sac. (interestingengineering.com)
The actin cytoskeleton is a very dynamic and labile structure in the living cell, but it can be fixed with paraformaldehyde prior to probing or staining for actin structures. (cytoskeleton.com)
They are 3-dimensional, complex, multicellular structures that can be derived from patient specific human induced pluripotent stem cells (hiPSCs). (nature.com)
Since the first publication on generating kidney-like cell aggregates from pluripotent stem cells, various modifications have been made to develop more complex and detailed kidney structures. (j-organoid.org)
In contrast to earlier models that featured nephron-like structures, these advances have improved the differentiation efficiency and similarity to the human kidney. (j-organoid.org)

Zernicka-Goetz's pioneering work in developing integrated stem cell-derived embryo models has garnered recognition, as she has been named the recipient of the esteemed 2023 Ogawa-Yamanaka Stem Cell Prize. (interestingengineering.com)

This embryonic swelling develops from the first pharyngeal arch and occurs midline on the floor of the developing pharynx, eventually helping form the tongue as the two lateral lingual swellings overgrow it. (medscape.com)

Alveolar type II (ATII) epithelial cells synthesize, secrete and recycle all components of surfactant and dysfunction in surfactant metabolism can result in a variety of pediatric lung diseases including respiratory distress syndrome and interstitial lung disease 3 . (nature.com)

Anatomy60

Organisms10

Diseases2

Chemicals and Drugs76

Analytical, Diagnostic and Therapeutic Techniques and Equipment44

Psychiatry and Psychology1

Phenomena and Processes59

Disciplines and Occupations4

Technology, Industry, Agriculture2

Humanities3

Information Science2

Health Care1

Proliferation and differentiation4

Mouse embryonic stem2

Murine5

Tissues18

Mesoderm8

Differentiate13

Embryos6

Vitro11

Early embryonic3

Stroma6

Development36

Undergoes6

Genes9

Tissue24

Endothelial cells3

Populations7

Growth12

Hematopoietic stem1

Lineages1

Morphogenesis5

Endoderm1

Mammary gland1

Epithelial cell4

Bilaminar1

Cranial3

Germ cells1

Adult stem1

Regulate5

Developmental2

Stromal5

Pluripotency1

Progenitor cells1

Fate3

Biol2

Cellular2

Regeneration2

Endodermal2

Follicular3

Chondrocytes1

Pathways1

Organs1

Differentiates1

Fetal2

Bone marrow2

Biology1

Neural stem1

Potential of stem1

Undergo2

Phenotype1

Thyroid1

Regenerative medicine1

Mice2

Mechanisms1

Renal repair2

Structures6

Embryo models1

Pharyngeal1

Metabolism1