• Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
  • In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
  • Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
  • Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
  • While multipotent mesenchymal stromal cells have been recently isolated from adult lung (L-MSCs), there is very limited data on their biological properties and therapeutic potential in vivo. (nih.gov)
  • In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
  • Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)
  • 1 Unique MSC-specific markers have not yet been identified, and MSCs constitute a heterogeneous cell population, including both multipotent (stem) cells and progenitor cells and might even contain pluripotent cell fractions. (bmj.com)
  • 2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)
  • 3 4 Since then, it has become evident that MSCs reside in many tissues, including mesenchymal tissues (bone, adipose tissue, connective tissue), umbilical cord and several organs including the liver, spleen and lung. (bmj.com)
  • Following early observations that MSCs inhibit T-cell proliferation, 9 MSCs were found to interact with the majority of innate and adaptive immune cells. (bmj.com)
  • Mesenchymal stromal cells (MSCs) show great promise for a wide range of applications including treating graft-vs-host disease, rheumatoid arthritis and cardiovascular diseases, as well as in tissue-engineering. (nanomelbourne.com)
  • This can be attributed to the direct differentiation of MSCs into the cells that form tissues such as bone or cartilage, or the activity of the factors that MSCs secrete which can have powerful regenerative properties. (nanomelbourne.com)
  • Mesenchymal stem/stromal cells (MSCs)‐based therapy brings the reassuring capability to regenerative medicine through their self‐renewal and multilineage potency. (biomedcentral.com)
  • Although there is no particular quantitative assay to provide MSCs identification in mixed cells population [ 8 ], the International Society for Cellular Therapy (ISCT) has provided minimum principles to determine MSCs. (biomedcentral.com)
  • However, natural MSCs in vivo survival and their biological effects on tissue recovery decrease with long-term cultivation called aging and also injected cells demonstrate poor targeted migration [ 21 ]. (biomedcentral.com)
  • In addition to their stem/progenitor properties, mesenchymal stromal cells (MSCs) possess broad immunoregulatory properties that are being investigated for potential clinical application in treating immune-based disorders. (pdffox.com)
  • Introduction Mesenchymal stromal cells (MSCs) are adult, fibroblast-like multipotent cells characterized by the ability to differentiate into tissues of mesodermal origin, such as adipocytes, chondroblasts, and osteoblasts (Friedenstein et al. (pdffox.com)
  • In response to this challenge, the International Society for Cellular Therapy formulated minimal criteria for defining MSCs in order to create a broader consensus for more uniform characterization of these cells (Dominici et al. (pdffox.com)
  • This ability of MSCs to adopt a different phenotype in response to sensing an inflammatory environment is not captured in assays that are commonly used to characterize these cells, but it is crucial for understanding their therapeutic potential in immune-mediated disorders. (pdffox.com)
  • Mesenchymal stem/stromal cells (MSCs) are a reservoir for tissue homeostasis and repair that age during organismal aging. (encyclopedia.pub)
  • MSCs represent a lifelong reservoir for the generation of somatic cells and for cell replacement. (encyclopedia.pub)
  • During aging of the organism, MSCs also age, and this implies an impairment of stem cell functions contributing to the progressive decrease in tissue maintenance and repair, a characteristic of the aging process. (encyclopedia.pub)
  • Due to the low abundance of MSCs in human adult tissues (about 1/10 6 cells in adult bone marrow and 1/10 3-4 cells in adipose tissue and umbilical cord) [ 16 ] , frequently ex-vivo expansion precedes therapeutic administration, to obtain a clinically relevant number of cells. (encyclopedia.pub)
  • Bone marrow mesenchymal stromal cells (BM-MSCs) may survive and proliferate in the presence of cycling neoplastic cells. (univr.it)
  • Exogenously administered MSCs are actively incorporated in the tumor as stromal fibroblasts, thus competing with the local mesenchymal cell precursors. (univr.it)
  • For this reason, MSCs have been suggested as a suitable carrier for gene therapy strategies, as they can be genetically engineered with genes encoding for biologically active molecules, which can inhibit tumor cell proliferation and enhance the anti-tumor immune response. (univr.it)
  • We used BM-MSCs engineered with the murine interferon-alpha (IFN-alpha) gene (BM-MSCs/IFN-alpha) to assess in a mouse plasmacytoma model the efficacy of this approach towards neoplastic plasma cells. (univr.it)
  • By contrast, intravenous administration of BM-MSCs/IFN-alpha did not significantly modify the survival of mice, mainly as a consequence of an excessive entrapment of injected cells in the pulmonary vessels. (univr.it)
  • Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (stemcellslab.net)
  • Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (stemcellslab.net)
  • Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (stemcellslab.net)
  • The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (stemcellslab.net)
  • and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (stemcellslab.net)
  • Mesenchymal stem cells (MSCs) are an adult stromal cell population possessing powerful differentiation capacity and a prospect of use across main histocompatibility complicated barriers. (biongenex.com)
  • Because of the immunological difficulties associated with allogeneic transplantation mesenchymal stem cells (MSCs) have the potential to be an attractive tissue replacement therapy for a number of reasons. (biongenex.com)
  • MSCs are multipotent cells that can be readily isolated from a number of adult tissues including bone marrow umbilical cord blood adipose tissue and placenta. (biongenex.com)
  • They have been well documented to differentiate into osteogenic adipogenic and chondrogenic lineages by activation of the cells through encountering inflammatory cytokines such as IFN-γ [21 23 Allogeneic MSCs from young healthy donors are an attractive source of regenerative cells for the treatment of degenerative diseases with an inflammatory component. (biongenex.com)
  • As MSCs possess potent immunomodulatory properties and an ability to differentiate into several lineages there is potential for allogeneic 'off the shelf' tissue engineering solutions using these cells. (biongenex.com)
  • Mesenchymal stromal cells (MSCs) constitute the cell type more frequently used in many regenerative medicine approaches due to their exclusive immunomodulatory properties, and they have been reported to mediate profound immunomodulatory effects in vivo. (bvsalud.org)
  • Nevertheless, MSCs do not express essential adhesion molecules actively involved in cell migration , a phenotypic feature that hampers their ability to home inflamed tissues following intravenous administration . (bvsalud.org)
  • Together, these findings indicate that exofucosylation could represent a suitable cell engineering strategy, not only to facilitate the in vivo MSC colonization of damaged tissues after systemic administration , but also to convert MSCs in a more potent immunomodulatory/anti-inflammatory cell therapy -based product for the treatment of a variety of autoimmune, inflammatory, and degenerative diseases . (bvsalud.org)
  • Mesenchymal stromal cells (MSCs) are a multipotent and multifunctional adult progenitor cell which have been extensively shown to improve islet function and viability in vitro. (kcl.ac.uk)
  • To explore how MSCs might affect β-cell biological processes in a broader sense we performed differential expression analysis with a lower fold change threshold of 1.1 (10% change). (kcl.ac.uk)
  • This suggests that MSCs are inducing loss of the mature β -cell phenotype/ a mild dedifferentiation which we predict may be protective to islets. (kcl.ac.uk)
  • Mesenchymal Stromal Cells (MSCs) are susceptible to tumor signalling and can be recruited to tumor regions. (unicyte.ch)
  • We found that CSC-derived EVs promoted persistent phenotypical changes in MSCs characterized by an increased expression of genes associated with cell migration (CXCR4, CXCR7), matrix remodeling (COL4A3), angiogenesis and tumor growth (IL-8, Osteopontin and Myeloperoxidase). (unicyte.ch)
  • Moreover, EV-stimulated MSCs enhanced migration of renal tumor cells and induced vessel-like formation. (unicyte.ch)
  • In vivo, EV-stimulated MSCs supported tumor development and vascularization, when co-injected with renal tumor cells. (unicyte.ch)
  • Bone marrow-derived mesenchymal stromal cells (MSCs) are known by their migratory capacity to injury and tumor sites. (unicyte.ch)
  • Anti- tumoral effects have been reported in different types of tumors, showing a reduction in tumor growth and metastasis with the presence of MSCs or EVs derived from these cells [15-18]. (unicyte.ch)
  • Such dual role possibly depends on a complex mechanism of interaction between tumor cells and MSCs that can be initially triggered by tumor EVs. (unicyte.ch)
  • The minimal criteria to define MSCs further include a specific set of cell surface markers. (wikipedia.org)
  • The cells must express CD73, CD90 and CD105 and they must be negative for CD14 or CD11b, CD34, CD45, CD79 alpha or CD19 and HLA-DR. Low levels of human leukocyte antigen (HLA-DR) make MSCs hypoimmunogenic. (wikipedia.org)
  • Because of their multi/pluripotency and immunosuppressive properties, mesenchymal stem/stromal cells (MSCs) are important tools for treating immune disorders and for tissue repair. (biomedcentral.com)
  • Producing cells according to Good Manufacturing Practices (GMPs) is a global challenge for the production of all cells for use in humans, specifically mesenchymal stem/stroma cells (MSCs), one of the most promising tools in cellular therapy and regenerative medicine. (biomedcentral.com)
  • MSCs with a mesodermic origin were first described by Alexander Friedenstein and colleagues in the 1960s and 1970s[ 1 ] as non-hematopoietic bone marrow (BM) cells that adhered to plastic and that could develop into colonies with a fibroblastic appearance. (biomedcentral.com)
  • Cells referred to as MSCs originating from various tissues are now used in clinical trials. (biomedcentral.com)
  • MSCs versus stem cell-based interventions other than MSCs. (lu.se)
  • Cell quality, as assessed by differentiation, cell surface marker expression and clonogenic ability, was retained post expansion on the liquid microcarriers. (aston.ac.uk)
  • They also can inspire angiogenesis by direct differentiation, cell-to-cell interaction, or paracrine effects. (biomedcentral.com)
  • These cells possess both differentiation plasticity (stemness) and tissue supportive functions (stromalness) that can coexist and overlap, with differences depending on tissue source, donor characteristics, culture conditions and delivery strategies, leading to alternative best fittings for the term "stem" or "stromal" [ 7 ] . (encyclopedia.pub)
  • As compared to 2D-expanded MSC (control), those derived from 3D-perfusion culture after the same time (3 weeks) or a similar extent of proliferation (7-8 doublings) better maintained their progenitor properties, as assessed by a 4.3-fold higher clonogenicity and the superior differentiation capacity towards all typical mesenchymal lineages. (unibas.ch)
  • The stromal cell system has been proposed to consist of marrow mesenchymal stem cells that are capable of self-renewal and differentiation into various connective tissue lineages. (nih.gov)
  • Recent data from in vitro models demonstrating the essential role of stromal support in hematopoiesis shaped the view that cell-cell interactions in the marrow microenvironment are critical for normal hematopoietic function and differentiation. (nih.gov)
  • They also aid in differentiation of hematopoietic cells and forming necessary blood elements. (wikipedia.org)
  • It is well known that stromal cells arise and are stored in the bone marrow until maturation and differentiation. (wikipedia.org)
  • Objective: To establish cultures of cells from the pulp of permanent teeth by the explant method assessing parameters usually presented by stem cells, such as the expression of certain markers and the differentiation ability into osteogenic, adipogenic, and chondrogenic lineages. (bvsalud.org)
  • In addition, cells were assessed for multi-differentiation potential. (bvsalud.org)
  • Similarly, the cells showed differentiation ability into all three lineages of cells tested. (bvsalud.org)
  • The isolated cells may be considered stem cells, based on the current criteria for their characterization, such as plastic adherence, expression of certain markers, and the absence of others, as well as multi-differentiation potential, which showed to be promising for the application in tissue regeneration. (bvsalud.org)
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
  • Taylor, S , Smith, R & Clegg, P 2009, ' Application of strain to co-cultures of equine tendon fibroblasts and mesenchymal stromal cells decreases tenascin-C and collagen type-V expression ', BSMB Spring Meeting: When, why and how did my cartilage go? (ed.ac.uk)
  • The most common stromal cells include fibroblasts and pericytes. (wikipedia.org)
  • The cells were first named colony-forming unit-fibroblasts (CFU-Fs). (biomedcentral.com)
  • Sex cord-stromal tumors are groups of tumors composed of granulosa cells, theca cells, Sertoli cells, Leydig cells, and fibroblasts of stromal origin, singly or in various combinations. (medscape.com)
  • These growth factors act on surrounding cells and stimulate chemotaxis of neutrophils, monocytes, and fibroblasts to the area of injury. (medscape.com)
  • bFGF is a chemotactic and mitogenic factor for fibroblasts and endothelial cells. (medscape.com)
  • When administered post-stroke, mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) induce neuroprotection, promote brain remodeling and plasticity, and enhance neurological recovery in rodents and non-human primates via mechanisms that involve immunomodulation and anti-inflammation. (explorationpub.com)
  • Recently, mesenchymal stromal cell (MSC)-derived small extracellular vesicles (EVs) have emerged as a potential new therapeutic agent for stroke treatment. (explorationpub.com)
  • Extracellular vesicles derived from renal cancer stem cells induce a pro-tumorigenic phenotype in mesenchymal stromal cells. (unicyte.ch)
  • CSCs were shown to secrete large amount of extracellular vesicles (EVs) that can transfer several molecules (proteins, lipids and nucleic acids) and induce epigenetic changes in target cells. (unicyte.ch)
  • In addition, tumor cells interaction were also shown to be mediated by secretion of extracellular vesicles (EVs) that can transfer proteins, lipids and nucleic acids and induce epigenetic changes in target cells [4,5]. (unicyte.ch)
  • Mesenchymal Stromal Cell-Derived Extracellular Vesicles in Lung Diseases: Current Status and Perspectives. (cdc.gov)
  • Recently, the adipose tissue-derived stromal vascular fraction has become the most popular source for obtaining mesenchymal stem cells because it is less expensive and is easier to perform than bone marrow concentrate harvesting. (balkanmedicaljournal.org)
  • However, no study has investigated the factors affecting the mesenchymal stem cell population in adipose tissue derived stromal vascular fraction. (balkanmedicaljournal.org)
  • Understanding the interaction of patient factors with the mesenchymal stem cell count and cell viability in adipose tissue-derived stromal vascular fraction could provide crucial information for surgeons to improve patient selection and outcomes. (balkanmedicaljournal.org)
  • To evaluate the factors affecting the mesenchymal stem cell count, total cell count, and cell viability in adipose tissue-derived stromal vascular fraction. (balkanmedicaljournal.org)
  • This study retrospectively reviewed the medical records of 30 patients who underwent liposuction to harvest adipose tissue-derived stromal vascular fraction at our stem cell center. (balkanmedicaljournal.org)
  • In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
  • In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
  • The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. (medsci.org)
  • Díaz-Prado, S.M. Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells. (medsci.org)
  • MSC use frequently requires in vitro expansion, thus exposing cells to replicative senescence. (encyclopedia.pub)
  • Combined data now show that splenic stroma of both human and murine origin provides a mesenchymal stromal cell microenvironment which is WNT pathway-dependent, and which supports in vitro myelopoiesis with production of specific subsets of myeloid and dendritic-like cells. (edu.au)
  • Research Objective Using authentic in vitro models of the human lung, complete with inflammatory cells & vessels, we will validate drugs that might be rapidly repurposed for use in patients with COVID-19. (ca.gov)
  • As a third major line of research, we explore, in collaboration with associate professor Henrik Ahlénius group if GBM cells can be reprogrammed to neuron like cells in vitro and in vivo. (lu.se)
  • Exosomes secreted by the umbilical cord mesenchymal stromal cells (UMSCs) and circular RNAs (circRNAs) derived from exosomes have been shown to be associated with the progression of GDM-related complications. (hindawi.com)
  • Numerous reports showed that tumor EVs are not restricted only to tumor microenvironment but are also present in the blood circulation and other body fluids, supporting the idea that EVs can also stimulate cells at distant sites in the organism [10-13]. (unicyte.ch)
  • molecules secreted by the tumor and tumor-related stromal cells, promoting their recruitment from bone marrow into the circulation and subsequent engraftment within tumor microenvironment [14]. (unicyte.ch)
  • Recent efforts demonstrated that the multiple mesenchymal lineages can be clonally derived from a single mesenchymal stem cell, supporting the proposed paradigm. (nih.gov)
  • Also, following transplantation and migration to target tissue, they encounter a harsh milieu accompanied by death signals because of the lack of proper tensegrity structure between the cells and matrix. (biomedcentral.com)
  • In these studies, however, transplanted cells were followed, if at all, at the site of transplantation, and biodistribution was not an issue. (stemcellslab.net)
  • This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (stemcellslab.net)
  • The replacement of β-cells through the transplantation of isolated pancreatic islets is a promising treatment for type 1 diabetes. (kcl.ac.uk)
  • Data from preclinical transplantation studies suggested that stromal cell infusions not only prevent the occurrence of graft failure, but they have an immunomodulatory effect. (nih.gov)
  • Preclinical and early clinical safety studies are paving the way for further applications of mesenchymal stem cells in the field of transplantation with respect to hematopoietic support, immunoregulation, and graft facilitation. (nih.gov)
  • We report here that Ptch +/- mice develop primordial follicular neoplasms resembling human trichoblastomas, and that exposure to ultraviolet radiation or ionizing radiation results in an increase in the number and size of these tumors and a shift in their histologic features so that they more closely resemble human basal cell carcinoma. (nature.com)
  • The mouse basal cell carcinomas and trichoblastoma-like tumors resemble human basal cell carcinomas in their loss of normal hemidesmosomal components, presence of p53 mutations, frequent loss of the normal remaining Ptch allele, and activation of hedgehog target gene transcription. (nature.com)
  • The Ptch mutant mice provide the first mouse model, to our knowledge, of ultraviolet and ionizing radiation-induced basal cell carcinoma-like tumors, and also demonstrate that Ptch inactivation and hedgehog target gene activation are essential for basal cell carcinoma tumorigenesis. (nature.com)
  • [ 1 ] sex cord-stromal tumors are classified into the following categories. (medscape.com)
  • Sex cord-stromal tumors account for approximately 8% of all ovarian tumors. (medscape.com)
  • Although various markers have been reported to stain sex cord-stromal tumors (eg, CD99, CD56, A103, müllerian inhibiting factor, vimentin), inhibin and calretinin have proven to be the most helpful to date. (medscape.com)
  • CD56 is a sensitive marker of ovarian sex cord-stromal tumors and may also be useful in the diagnosis of this group of neoplasms, especially in cases that are inhibin or calretinin negative, and the differential diagnosis includes neoplasms that are CD56 negative. (medscape.com)
  • A worldwide database analysis revealed that younger age, smaller tumor size, early stage, and granulosa cell tumor histologic type appear to be independent prognostic factors for improved survival in patients with malignant sex cord-stromal tumors who undergo lymph node dissection. (medscape.com)
  • Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)
  • Tissue from both primary surgery and reoperations from the same patients, allows in depth analysis of the evolution of glioblastoma over time with the goal of development of diagnostic monitoring and identification of druggable GBM stromal targets in recurrent brain tumors. (lu.se)
  • Gastrointestinal stromal tumors are tumors of the gastrointestinal tract derived from mesenchymal precursor cells in the gut wall. (msdmanuals.com)
  • Gastrointestinal stromal tumors (GISTs) result from mutations of a growth factor receptor gene, C-KIT . (msdmanuals.com)
  • Gastrointestinal stromal tumors (GISTs) result from mutations. (msdmanuals.com)
  • Equine osteoarthritis: strategies to enhance mesenchymal stromal cell-based acellular therapies. (cabi.org)
  • These factors make it an effective tool in potential cell therapies and tissue repair. (wikipedia.org)
  • Stem cell-based therapies offer a potential therapeutic approach to repair, restore, or regenerate injured brain tissue. (lu.se)
  • Published clinical studies of cell therapies and gene therapies date back at least 15 years, according to a review by Mellerio on why developments are starting to move so quickly. (medscape.com)
  • In addition to cell therapies and gene therapies, Mellerio discussed progress in three additional areas: gene editing, protein therapy, and drug repurposing. (medscape.com)
  • They are located in the stroma and aid hematopoietic cells in forming the elements of the blood. (wikipedia.org)
  • The cross-talk between the host stroma and tumor cells is essential for tumor growth and progression. (wikipedia.org)
  • The applicant is responsible for a brain tumor tissue biobank which provides the basis for the studies on human GBM tumor cells and stroma. (lu.se)
  • Our lab have, at the cutting edge of this research area, systematically been studying the mesenchymal stroma of GBM during the past years. (lu.se)
  • Mesenchymal stromal cells: a novel therapy for the treatment of chronic obstructive pulmonary disease? (bmj.com)
  • 1. Cell Therapy and Regenerative Medicine Unit, Rheumatology Group, Institute of Biomedical Research of A Coruña (INIBIC), University Hospital Complex A Coruña (CHUAC), Galician Health Service (SERGAS), Department of Medicine, Faculty of Health Sciences, University of A Coruña, A Coruña, Spain. (medsci.org)
  • Stem cell therapy has been the subject of many studies for its potential to cure many disorders. (biomedcentral.com)
  • Find out what potential options you have with stem cell therapy. (stemedix.com)
  • Mesenchymal stromal/stem cell (MSC) expansion in conventional monolayer culture on plastic dishes (2D) leads to progressive loss of functionality and thus challenges fundamental studies on the physiology of skeletal progenitors, as well as translational applications for cellular therapy and molecular medicine. (unibas.ch)
  • Therapeutic Candidate or Device Novel testing of a cell based therapy (Mesenchymal Stromal Cells) for respiratory failure from ARDS. (ca.gov)
  • Research Objective We expect to generate iPSC derived NK cells expressing a CAR against SARS-cov2 that could be used as an off-the-shelf therapy for COVID-19 Impact The proposed studies will provide a novel therapeutic approach to boost the cellular immunity against SARS-cov2, especially for high risk populations. (ca.gov)
  • Furthermore, TASCs secrete many pro-tumorigenic factors such as vascular endothelial growth factor (VEGF), stromal-derived factor-1 alpha, IL-6, IL-8, tenascin-C, and others. (wikipedia.org)
  • Additionally, the recruitment of local normal host stromal cells, such as bone marrow mesenchymal stromal cells, endothelial cells, and adipocytes, help create a conspicuously heterogeneous composition. (wikipedia.org)
  • IL-1 stimulates the proliferation of inflammatory cells and promotes angiogenesis through endothelial cell replication. (medscape.com)
  • This prompted further sub-clustering of β-cells into mature and immature-like populations based on expression of key β-cell maturity markers. (kcl.ac.uk)
  • When human progenitor populations from spleen, bone marrow and cord blood were employed as a source of progenitors for co-culture above splenic stromal lines, myelopoiesis was supported. (edu.au)
  • The tumor is composed by different cell populations. (unicyte.ch)
  • A subset of tumor cells, defined as cancer stem cells (CSCs), are characterized by self-renewal and continuous proliferation capacity, providing the ability to initiate tumor and to generate other heterogeneous cell populations [1]. (unicyte.ch)
  • Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
  • Human amniotic membrane and amniotic membrane-derived mesenchymal stromal cells (hAMSCs) have produced promising results in regenerative medicine, especially for the treatment of inflammatory-based diseases and for different injuries including those in the orthopedic field such as tendon disorders. (mdpi.com)
  • Plasma and synovial fluid cell-free DNA concentrations following induction of osteoarthritis in horses. (cabi.org)
  • Implication of cellular senescence in osteoarthritis: a study on equine synovial fluid mesenchymal stromal cells. (cabi.org)
  • Frontiers in cell and developmental biology 2021 0 0. (cdc.gov)
  • Clinical Relevance: The harvest technique of BM may be of major importance in determining the clinical success of BM mesenchymal stem/stromal cells in cartilage repair. (whiterose.ac.uk)
  • Impressively, four of the six patients-for whom no other multiple sclerosis treatment worked-had a measurable clinical improvement after stem cell treatment. (stemedix.com)
  • Based on the results of this clinical study, the scientists concluded that neural progenitor cells created from autologous mesenchymal stromal cells were safe to use in patients with primary progressive multiple sclerosis. (stemedix.com)
  • 2016). Clinical safety of intrathecal administration of mesenchymal stromal cell-derived neural progenitors in multiple sclerosis. (stemedix.com)
  • The relationship of patient factors (age, body mass index, hypertension, and coronary artery disease) with cell viability and mesenchymal stem cell counts may be important for clinical applications. (balkanmedicaljournal.org)
  • The researchers provided between 2 to 5 infusions of neural progenitor cells into the spinal fluid. (stemedix.com)
  • After that, Kaplan firstly proposes the term "mesenchymal stem cells," which are cells isolated from fully developed bone marrow (BM) that can usually differentiate into several types of mesenchymal origin cells [ 4 ]. (biomedcentral.com)
  • Results: The isolated cells showed high expression of CD44 (98.8%), CD73 (100%), and CD90 (97.2%), and moderate expression of STRO-1 (18.4%) and ALDH1 (16.2%), by flow cytometry. (bvsalud.org)
  • Background: Connective tissue progenitors (CTPs) from native bone marrow (BM) or their culture-expanded progeny, often referred to as mesenchymal stem/stromal cells, represents a promising strategy for treatment of cartilage injuries. (whiterose.ac.uk)
  • Stromal cells can become connective tissue cells of any organ, for example in the uterine mucosa (endometrium), prostate, bone marrow, lymph node and the ovary. (wikipedia.org)
  • Being a mesenchymal cell indicates an ability to develop into various other cell types and tissues such as connective tissue, blood vessels, and lymphatic tissue. (wikipedia.org)
  • This study also aimed to assess the expression of ALDH1 (aldehyde dehydrogenase 1) enzyme activity on the isolated cells. (bvsalud.org)
  • Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)
  • Conclusion: Our results suggest that the explant method - or cell proliferation method - is suitable for the isolation and culture of stem cells from dental pulp of permanent teeth. (bvsalud.org)
  • The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. (mdpi.com)
  • BACKGROUND: Traditional large-scale culture systems for human mesenchymal stem/stromal cells (hMSCs) use solid microcarriers as attachment substrates. (aston.ac.uk)
  • Basal cell carcinomas, the commonest human skin cancers, consistently have abnormalities of the hedgehog signaling pathway and often have PTCH gene mutations. (nature.com)
  • Mutations of the human homolog of Drosophila patched in the nevoid basal cell carcinoma syndrome. (nature.com)
  • Human homolog of patched, a candidate gene for the basal cell nevus syndrome. (nature.com)
  • The role of the human homologue of Drosophila patched in sporadic basal cell carcinomas. (nature.com)
  • Patched (ptch)-associated preferential expression of smoothened (smoh) in human basal cell carcinoma of the skin. (nature.com)
  • Similar stromal lines have now been isolated from long-term cultures (LTC) of human spleen. (edu.au)
  • Human splenocytes gave production of predominantly myeloid dendritic-like cells, with minor subsets resembling conventional dendritic cells (cDC) cells, and myeloid or monocyte-derived DC. (edu.au)
  • Research Objective To optimize a new approach to deliver Anti-COVID siRNAs into human iPSC-derived lung cells that can selectively kill the COVID virus Impact Our proposal, if successful, will solve the siRNA delivery problem and rapidly open the door to Anti-COVID siRNA therapeutics. (ca.gov)
  • Therapeutic Candidate or Device Human placental hematopoietic stem cell derived natural killer cells (CYNK-001) Indication SARS-CoV-2 positive patients requiring hospital admission and have any 2 out of 3 symptoms: fever, cough, or positive disease-related chest x-ray. (ca.gov)
  • Effects of agricultur al organic dusts on human lung-resident mesenchymal stem (stromal) cell function. (cdc.gov)
  • We have already acquired proof-of-principle showing that reprogramming towards astrocytic fate causes morphological changes, reduces proliferation and bestow astrocytic function in human GBM cell lines. (lu.se)
  • Sozzi E, Nilsson F, Kajtez J, Parmar M, Fiorenzano A. Generation of Human Ventral Midbrain Organoids Derived from Pluripotent Stem Cells . (lu.se)
  • To circumvent these adversities in the delivery of a viral vector to the site of pathology, mesenchymal stem/stromal cells may be used to avoid immune stimulation with resultant vector clearance, to maximize the effective delivery potential, and to augment biodistribution ability in neoplastic tumor masses. (northwestern.edu)
  • Results: Cells from the BM after rasping were significantly more chondrogenic than the donor-matched aspirate, whereas no notable difference in their osteogenic or adipogenic potential was observed. (whiterose.ac.uk)
  • Gene profiling revealed both stromal lines to reflect perivascular reticular cells with osteogenic characteristics. (edu.au)
  • The interaction between stromal cells and tumor cells is known to play a major role in cancer growth and progression. (wikipedia.org)
  • Materials and method: The pulp tissue, obtained from wisdom teeth, was placed in a 6-well plate containing proper culture medium, and stored at 37 °C and 5% CO2 for cell proliferation and plastic adherence. (bvsalud.org)
  • IL-2 promotes further T cell proliferation to augment the immunogenic response to injury. (medscape.com)
  • American journal of respiratory cell and molecular biology 2022 0 0. (cdc.gov)
  • The anti-tumor effect was associated with enhanced apoptosis of tumor cells, reduction in microvessel density, and ischemic necrosis. (univr.it)
  • However, under certain conditions, tumor cells can convert these reactive stromal cells further and transition them into tumor-associated stromal cells (TASCs). (wikipedia.org)
  • Here we demonstrate that 2D MSC expansion can be entirely bypassed by culturing freshly isolated bone marrow nucleated cells within 3D porous scaffolds in a perfusion-based bioreactor system. (unibas.ch)
  • Moreover, the histological response showed that the scaffolds are biocompatible and those sterilized by GR showed a more severe inflammatory response, accompanied with the presence of giant foreign body cells. (bvsalud.org)
  • Operative variables, such as lipoaspirate amount and donor areas from the stromal vascular fraction harvesting site, included the entire abdomen and lower abdomen. (balkanmedicaljournal.org)
  • We recorded the mesenchymal stem cell population, cell viability, and cell count of stromal vascular fraction, and we analyzed the results to determine statistical significance. (balkanmedicaljournal.org)
  • Then, in their laboratory, scientists used various means to prompt the cells to become neural progenitors. (stemedix.com)
  • A neural progenitor cell is a cell that can become any of the three main types of brain cells: neurons, astrocytes, or oligodendrocytes. (stemedix.com)
  • Purdon, S & Glassberg, MK 2019, ' Multipotent mesenchymal stromal cells and lung disease: Not ready for prime time ', Annals of the American Thoracic Society , vol. 16, no. 6, pp. 669-671. (arizona.edu)
  • Telomere Shortening and Its Association with Cell Dysfunction in Lung Diseases. (cdc.gov)
  • Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. (mdpi.com)
  • But the cartilage regeneration capacity of these cells remains unpredictable because of cell heterogeneity. (whiterose.ac.uk)
  • Hypothesis: The harvest technique of BM may highly influence stem cell heterogeneity and, thus, cartilage formation because these cells have distinct spatial localization within BM from the same bone. (whiterose.ac.uk)
  • In this study, we investigated whether modification by fucosylation of murine AdMSCs (mAdMSCs) creates Hematopoietic Cell E-/ L-selectin Ligand , the E-selectin -binding CD44 glycoform. (bvsalud.org)
  • Dexter demonstrated in 1982 that an adherent stromal-like culture was able to support maintenance of hematopoietic stem as well as early B lymphopoeisis. (nih.gov)
  • Maintenance of the hematopoietic stem cell population has been used to increase the efficiency of hematopoietic stem cell gene transfer. (nih.gov)
  • High-dose chemotherapy and frequently cause stromal damage with resulting hematopoietic defects. (nih.gov)
  • To investigate this, we aimed to induce dedifferentiation in β-cells through several methods: FGF2 treatment, inhibition of Foxo1, and siRNA KD of Ins2, Mafa, Slc2a2 and Nkx6.1 . (kcl.ac.uk)
  • They are cells that support the function of the parenchymal cells of that organ. (wikipedia.org)