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Induced Pluripotent Stem CellsPluripotent Stem CellsStem CellsMesenchymal Stem Cell TransplantationMesenchymal Stromal CellsEmbryonic Stem CellsCell DifferentiationNuclear ReprogrammingStem Cell TransplantationAdult Stem CellsOctamer Transcription Factor-3Cell Culture TechniquesHematopoietic Stem CellsCell LineageCells, CulturedRegenerative MedicineSOXB1 Transcription FactorsCell DedifferentiationNeural Stem CellsCell ProliferationEmbryoid BodiesMultipotent Stem CellsStem Cell NicheBone Marrow CellsCell- and Tissue-Based TherapyRegenerationStem Cell ResearchTissue EngineeringTeratomaHematopoietic Stem Cell TransplantationCell LineFibroblastsOsteogenesisCell SeparationGerm LayersStage-Specific Embryonic AntigensChondrogenesisFeeder CellsLentivirusFlow CytometryKruppel-Like Transcription FactorsCoculture TechniquesGene Expression RegulationGene Expression ProfilingFetal Stem CellsNeuronsMice, Inbred C57BLTissue ScaffoldsReverse Transcriptase Polymerase Chain ReactionMyocytes, CardiacSignal TransductionAdipogenesisGene Expression Regulation, DevelopmentalMice, SCIDEmbryo, MammalianBiological MarkersStem Cell FactorCell SurvivalCell ShapeTransduction, GeneticTranscription FactorsEpigenesis, GeneticHomeodomain ProteinsGenetic VectorsNeurogenesisMammalsImmunohistochemistryPhenotypeGerm CellsModels, BiologicalNestinGreen Fluorescent ProteinsGene ExpressionCell TransdifferentiationUmbilical CordNeoplastic Stem CellsAlkaline PhosphataseOsteoblastsTime FactorsCell TransplantationHematopoiesisColony-Forming Units AssayCell MovementTransgenesMice, TransgenicKaryotypingTotipotent Stem CellsAntigens, CD34RNA, MessengerProto-Oncogene Proteins c-mycCulture Media, ConditionedBone RegenerationFetal BloodPlant StemsNerve Tissue ProteinsBone Morphogenetic Protein 2Disease Models, AnimalHematopoietic Stem Cell MobilizationMice, Inbred NODNerve Regeneration
VitroIPSCsStromal cellsRegenerationNeural stemMSCsMultipotentTherapeuticTissuesRegenerativeIPSCTransplantationAbility to differentiateDifferentiateTissueESCsSomatic and pluripotent cellsPluripotencyBiologyCharacterizationBoneHematopoietic stemEmbryonic and adult stemMurineEmbryosEpithelial cellsHumanTranscriptionalAutologousAbstractUmbilical cordLineagesProfiles of somatic cellsInducing differentiated somatic cellsNeuronsTherapiesFunctionalAdherent2017IntestinalOrganoidsPancreatic cellsNicheProgenitorsPrecursor cellsDonorEndothelial CellsTherapyProliferation and differentiationIsolationRegulateTypesGermDifferentiation of stem cells
Vitro27
  • 2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
  • The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
  • Universal Cardiac Induction of Human Pluripotent Stem Cells in Two and Three-Dimensional Formats: Implications for In Vitro Maturation. (biodonostia.org)
  • Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile. (biodonostia.org)
  • These properties can be illustrated with relative ease in vitro, using methods such as clonogenic assays, where the progeny of a single cell is characterized. (wikipedia.org)
  • However, it is known that in vitro cell culture conditions can alter the behavior of cells, proving that a particular subpopulation of cells possesses stem cell properties in vivo is challenging, and so considerable debate exists as to whether some proposed stem cell populations in the adult are indeed stem cells. (wikipedia.org)
  • It can be induced by modifying the growth medium when stem cells are cultured in vitro or by transplanting them to an organ of the body different from the one they were originally isolated from. (wikipedia.org)
  • Some of these techniques include novel tools for genetic manipulation, 4,5 approaches for in vitro disease modelling 6-9 and innovative co-culture system with autologous cell types 10,11 or bacteria, 12-14 as well as viral infection models. (stemcell.com)
  • Adult stem cell-based organoid technology is a versatile tool for the generation and long-term maintenance of near-native 3D epithelial tissues in vitro. (nature.com)
  • In vitro generation of human pluripotent stem cell derived lung organoids. (nature.com)
  • Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis. (nature.com)
  • However, the limited amount of stem cells and their tendency to undergo spontaneous differentiation upon extended propagation in vitro restrict their practical application. (biomedcentral.com)
  • In addition, we found that in the presence of LIF, MDLS could replace feeder cells to maintain the undifferentiated state of OG2-mES cells (Oct4-GFP reporter gene mouse embryonic stem cell line), and the MDLS-expanded OG2-mES cells showed an elevated expression levels of pluripotency markers in vitro. (biomedcentral.com)
  • However, one of the bottlenecks in the stem cell therapy is that stem cells tend to differentiate into other types of cells during culture in vitro. (biomedcentral.com)
  • Therefore, finding a way to effectively maintain the pluripotency of stem cells cultured in vitro is important for the application of stem cells. (biomedcentral.com)
  • Differentiation is triggered by various factors in vivo , some of which can be replicated in in vitro stem cell cultures. (sigmaaldrich.com)
  • These protocols, such as organoid cell culture methods, have provided more predictive in vitro cellular "Disease-in-a-Dish" models. (sigmaaldrich.com)
  • The ability to expand pluripotent cells in vitro and subject them to direct differentiation to produce specific cell types is crucial to the development of cell-based therapies to replace or restore tissue that has been damaged by disease or injury. (sigmaaldrich.com)
  • 6 ] successfully isolated OGSCs from the ovaries of pups and adult mice for the first time, transfected them with GFP virus in an appropriate environment in vitro, and then transplanted them into the ovaries of immunodeficient mice. (biomedcentral.com)
  • The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
  • 2011 Latest generally in vitro research have implicated many EMT-inducing transcription elements (EMT-TFs) such as for example Snail Slug and Zeb1 as critical indicators that promote stemness in regular and malignant mammary epithelial cells (MECs) (Chaffer et al. (buyresearchchemicalss.net)
  • This study aims to provide information to answer the following question: While some scientists claim they can indefinitely culture a stem cell line in vitro, what are the consequences of those culturing practices? (asu.edu)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • Until recently, it was believed that they were tissue-specific…however, this concept has been challenged… (multipotent cells) can differentiate in vitro and in vivo into various cell types not only from the tissue of origin" (Can/Hematol 2008). (papersowl.com)
  • Marking a vast leap in reproductive therapy, it became possible to isolate these human embryonic stem cells for reproductive purposes, such as in vitro fertilization. (papersowl.com)
  • We assessed the effects of CD8 T cells and cytokine IFN-γ on AT2 cell differentiation capacity using the AT2- CD8 T cell co-culture system in vitro. (bvsalud.org)
  • Using biologically relevant elastic substrates to study cell function in vitro has proven beneficial, as the in vivo microenvironment usually is much softer than rigid plastic dishes. (5dok.org)
IPSCs12
Stromal cells7
  • Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
  • Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
  • Multipotent mesenchymal stem/stromal cells (MSCs) have regenerative and immunomodulatory properties to restore and repair injured tissues, making them attractive candidates for cell-based therapies. (shengsci.com)
  • The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
  • MSCs are multipotent stromal cells that make and restore skeletal tissue and can differentiate into diverse cell types: bone, cartilage, muscle and fat. (mastercellbank.com)
  • Defining essential stem cell characteristics in adipose-derived stromal cells extracted from distinct anatomical sites. (ui.ac.id)
  • We have recently reported that mechanical manipulation of human adipose tissue without modification of tissue composition, structure and histology, that was totally comparable to that of the original source (see Experimental procedure for details), increased significantly the number of stromal cells bearing the expression of beta-tubulin III. (neurogelenmarche-usa.org)
Regeneration26
  • To further understand the epigenetic regulators for specific lineage differentiation from iPS cell would have great significance for potential regeneration therapy and human disease modeling [ 8 ]. (biomedcentral.com)
  • 17 This property of rapid regeneration at intestinal stasis makes the intestine a uniquely convenient model system for epithelial cell biology and adult stem cell biology studies both inside and outside the specific context of intestinal function. (stemcell.com)
  • Apoptotic cells represent a dynamic stem cell niche governing proliferation and tissue regeneration , Stem Cell Reports. (ipscell.com)
  • These properties provide stem cells with powerful capabilities for tissue repair, replacement, and regeneration, so human stem cells are of special interest in medical research. (sigmaaldrich.com)
  • This review considers the genetic and epigenetic control of nephrogenesis, together with the epigenetic mechanisms that accompany kidney development and recent advances in induced reprogramming and kidney cell regeneration in the context of DN. (emjreviews.com)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • During limb epimorphic regeneration: committed mesenchymal cells at the stump site dedifferentiate, forming a cluster of heterogeneous population of stem cells, known as the blastema. (eurekaselect.com)
  • Epimorphic regeneration and retrodifferentiation both have the capacity to recreate and reconstruct tissue with precise positional integration of cells in such a way that will enable us to heal without scars and to understand how to maintain tissue integrity and architecture in the face of a hostile environment. (eurekaselect.com)
  • These cells play a critical role in the regeneration and restoration of injured sites. (shengsci.com)
  • 2012 Nevertheless the in vivo systems that restrict epithelial lineage plasticity to guard differentiation and exactly how such systems control stem cell function during MG morphogenesis and regeneration stay poorly understood. (buyresearchchemicalss.net)
  • Right here we offer in vivo proof to get a previously unrecognized system that defends epithelial identification during mammary tissues morphogenesis and regeneration that involves Ovo-like 2 (Ovol2) an associate from the Ovo category of zinc finger TFs that are recognized to regulate epithelial advancement in epidermis aswell as mammalian epidermis and testis (Dai et al. (buyresearchchemicalss.net)
  • Mouse utricle sensory epithelial cellCderived progenitor cells (MUCs), which possess locks cell progenitor and mesenchymal features via epithelial-to-mesenchymal changeover (EMT) seeing that previously described, provide a potential strategy for locks cell regeneration via cell transplantation. (forgetmenotinitiative.org)
  • In latest years, regeneration of locks cells offers produced guaranteeing outcomes (Groves et al. (forgetmenotinitiative.org)
  • The regeneration is maintained by adult stem cells. (biomedcentral.com)
  • Consistent with this activity, functional analyses in mice suggest that Lin28B plays an important role in hair cell development and regeneration. (encyclopedia.pub)
  • In neonatal murine cochlear organoids and explants, Lin28B antagonizes the activity of let7 miRNA and increases Akt-mTORC1 signaling to promote hair cell regeneration from immature supporting cells by inducing their de-differentiation and proliferation as well as by directly converting them into hair cells [ 3 ] . (encyclopedia.pub)
  • Thus, Lin28B functions in hair cell regeneration through mitotic and non-mitotic mechanisms, which are dependent on mitotic division or trans-differentiation of supporting cells into hair cells, respectively. (encyclopedia.pub)
  • This suggests that coactivation of Lin28B and Follistatin may represent an endogenous mechanism mediating reprogramming of supporting cells for hair cell regeneration. (encyclopedia.pub)
  • Lin28A is required for hair cell regeneration in the mammalian cochlea, and may function in redundant processes with Lin28B [ 3 ] [ 5 ] . (encyclopedia.pub)
  • Introduction: Alveolar epithelial regeneration depends on the activity of resident quiescent progenitor cells. (bvsalud.org)
  • Although AT2 cell plasticity has been a very intense field of research, the role of CD8 T cell response and their released cytokine IFN-γ, in regulating AT2 cell plasticity and alveolar epithelial repair and regeneration after injury remains largely unknown. (bvsalud.org)
  • To determine whether CD8 T cells and their released cytokine IFN-γ are necessary for AT2 cell activity during alveolar epithelial regeneration, we performed loss of function studies using anti-CD8 or anti-IFN-γ monoclonal antibody (mAb) treatment in vivo. (bvsalud.org)
  • Results: We detected a transient wave of accumulation of CD8 T cells in mouse lungs, which coincided with the burst of AT2 cell proliferation during alveolar epithelial repair and regeneration in mice following bacterial pneumonia caused by Streptococcus pneumoniae. (bvsalud.org)
  • Discussion: Our data demonstrate that CD8 T-cell response and cytokine IFN-γ are necessary for promoting AT2 cell activity during alveolar epithelial repair and regeneration after acute lung injury caused by bacterial pneumonia. (bvsalud.org)
  • 1999). Recent studies have shown that the potentiality of tissue regeneration can be enhanced using adipose derived stem cells (hADSCs) (see Gimble et al. (neurogelenmarche-usa.org)
  • The present study results indicated that compared with the same number of 2D-cultured cells, microtissue could secrete more nerve regeneration related cytokines to promote SCs proliferation and axons growth. (biomedcentral.com)
Neural stem2
MSCs6
  • Embryonic stem cells (ESCs) are pluripotent, signifying that they have the ability to differentiate into all adult cell types, while mesenchymal stem cells (MSCs) are multipotent and are more limited in their differentiation capacity. (videohall.com)
  • The authors reviewed the biology of three types of pluripotent stem cells, embryonic stem cells, or ESCs, mesenchymal stem cells, or MSCs, and induced pluripotent stem cells, or iPS cells. (asu.edu)
  • MSCs were cultured in defined, MesenCult-ACF media (StemCell Technologies, Cat. (nih.gov)
  • Mesenchymal stem cells (MSCs) the most significant stem cells for cell therapy were first isolated and characterized in 1974 and have been used in the clinic since 2004. (mastercellbank.com)
  • Adipose tissue derived mesenchymal stem cells (AT-MSCs) are relatively easy in isolation procedure compared to bone marrow-derived. (ui.ac.id)
  • This experimental study used cryopreserved adipose tissue derived MSCs stored in Stem Cell Medical Technology Integrated Service Unit Cipto Mangunkusumo Hospital. (ui.ac.id)
Multipotent6
  • Identification of multipotent luminal progenitor cells in human prostate organoid cultures. (nature.com)
  • The basal/myoepithelial inhabitants of adult MG provides the so-called multipotent mammary stem cells (MaSCs) that upon transplantation can handle regenerating a whole epithelial network made up of both luminal and basal/myoepithelial lineages (Shackleton et al. (buyresearchchemicalss.net)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • To delve further into the specifics, embryonic and adult stem cells fall into potency categories reflecting their ability to differentiate: totipotent, pluripotent, multipotent, and unipotent. (papersowl.com)
  • Multipotent stem cells are capable of forming many, but not all, cell types of the body. (papersowl.com)
  • Using these definitions, embryonic stem cells can be classified as totipotent or pluripotent at different developmental stages and locations, whereas adult stem cells can be categorized as pluripotent, multipotent, or unipotent (Figure 1). (papersowl.com)
Therapeutic13
  • There is yet no consensus among biologists on the prevalence and physiological and therapeutic relevance of stem cell plasticity. (wikipedia.org)
  • The lymphoma application segment leads the global stem cell banking market, owing to rising prevalence of these disorders and higher number of successful therapeutic procedures carried out to treat the condition using stem cells. (medgadget.com)
  • The present invention provides stem cells enriched with healthy functional mitochondria, and therapeutic methods utilizing such cells for the alleviation of debilitating conditions, including aging, and age-related diseases as well as the debilitating effects of anti-cancer therapies in subjects in need thereof. (justia.com)
  • The present invention relates to stem cells enriched with functional mitochondria, and therapeutic methods utilizing such cells to diminish the debilitating effects of various conditions, including aging and age-related diseases as well as the debilitating effects of anti-cancer therapy treatments. (justia.com)
  • The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • Studies have increasingly focused on the potential therapeutic effects of stem cell transplantation for neurological diseases [ 10 ]. (hindawi.com)
  • Dr. Bo Chen's research focuses on mechanistic and therapeutic studies of retinal degenerative diseases caused by loss of photoreceptors or retinal ganglion cells, such as age-related macular degeneration, retinitis pigmentosa, and glaucoma. (nih.gov)
  • The ability of stem cells to originate every tissue under the proper physiological or experimental conditions has made them a subject of great interest, particularly with respect to their therapeutic potential. (bio-rad-antibodies.com)
  • Before delving into the molecular biology and therapeutic potentials of induced pluripotent stem cells, it is crucial to provide foundational definitions and descriptions. (papersowl.com)
  • Mesenchymal stem cells: immunology and therapeutic benefits. (ui.ac.id)
  • 2011. http://www.intechopen.com/books/stem-cells-in-clinic-and-research/mesenchymal-stem-cells-immunology-and-therapeutic-benefits . (ui.ac.id)
Tissues17
  • Adult stem cells are undifferentiated cells, found throughout the body after development, that multiply by cell division to replenish dying cells and regenerate damaged tissues. (wikipedia.org)
  • In 2016, Axiogenesis AG and Metrion Biosciences Ltd., joined forces with the aim to validate, optimize, and commercialize induced pluripotent stem cell-derived cells and tissues to speed up research in drug discovery. (medgadget.com)
  • whereas, adult stem cells are isolated from adult tissues. (firebaseapp.com)
  • Stem cells represent a small fraction of cells located in different tissues. (shengsci.com)
  • In addition cIAP1 Ligand-Linker Conjugates 14 to HSC, bone marrow contains mesenchymal stem/progenitor cells (MSC), which can differentiate into mature cells of multiple mesenchymal tissues cIAP1 Ligand-Linker Conjugates 14 including fat, bone, and cartilage. (eyesoftheelephants.com)
  • 2009 whereas incomplete and reversible EMT takes place during morphogenesis of specific epithelial tissues such as for example mammary gland WYE-354 (MG) (Nakaya and Sheng 2013 Although very much has been learned all about the molecular systems that promote EMT during early advancement and in tumor cells hereditary pathways that regulate incomplete EMT during tissues morphogenesis to keep epithelial lineages are badly characterized. (buyresearchchemicalss.net)
  • Robert describes part-human animals, otherwise known as chimeras, as those resulting from the intentional combination of human and nonhuman cells, tissues, or organs at any stage of development. (asu.edu)
  • He predicted that cancers occur upon activation of dormant embryonic-like cancerous cells present in mature tissues but not spontaneously [ 8 ]. (biomedcentral.com)
  • Totipotent stem cells contain all the constituents necessary to produce a living being, given that these cells can supply all embryonic and extraembryonic tissues required for proper growth (Can/Hematol 2008). (papersowl.com)
  • In the 1960s and 1970s, scientists discovered that there are cells within adult tissues of the body that harbor many of the same special properties as embryonic stem cells. (pas.va)
  • However, these adult stem cells have more restricted potential - they are specialized to replenish, rejuvenate, and repair the tissues in which they reside. (pas.va)
  • embryonic stem cells , resulting from the early divisions of the egg, characterized by their "pluripotency", i.e. the capacity, that they share with the egg cell itself, to produce all the cell types found in the adult organism, and the tissue-specific stem cells present in the tissues and organs of the adult. (pas.va)
  • They are particularly active in tissues and organs in which the lifespan of the differentiated cells is short, like blood, skin and the inner cell layer covering the intestinal cavity, as well as in the repair of skeletal muscle after exercise of injury. (pas.va)
  • Significant advances have been made in isolating, culturing and reintroducing adult stem cells into tissues. (pas.va)
  • 2011). Stem cell frequency appears to be significantly higher in adipose tissue than in other tissues such as bone marrow (Bieback et al. (neurogelenmarche-usa.org)
  • The wide range of manifestations observed in patients with mitochondrial disease results from varying fractions of abnormal mtDNA molecules in different cells and tissues, a phenomenon termed heteroplasmy. (jci.org)
  • However, the landscape of heteroplasmy across cell types within tissues and its influence on phenotype expression in affected patients remains largely unexplored. (jci.org)
Regenerative10
IPSC3
  • Tra 1-60+ iPSC colonies were picked and re-plated on matrigel coated 12-well plates and maintained in mTeSR 1 (Stemcell Technologies, 85850). (nih.gov)
  • Induced pluripotent stem cells (iPSC) can now be generated from skin or blood of mice or humans by overexpressing four key transcription factors. (pas.va)
  • Human iPSC derived cardiovascular and neuronal cells are being used in preclinical studies and will find use in clinical application as cell therapies. (mastercellbank.com)
Transplantation9
  • WO 2001/046401 discloses embryonic or stem-like cells produced by cross species nuclear transplantation. (justia.com)
  • Clare's lab recently identified a population of progenitor/stem cells in mice that, on transplantation, is sufficient to generate a fully functional thymus. (eurostemcell.org)
  • Human induced pluripotent stem cells differentiation into oligodendrocyte progenitors and transplantation in a rat model of optic chiasm demyelination. (1library.org)
  • Impact of Diabetes Mellitus on Human Mesenchymal Stromal Cell Biology and Functionality: Implications for Autologous Transplantation. (shengsci.com)
  • gene transfer, hematopoietic stem cells, lentiviral vectors, mesenchymal stem/progenitor cells, bone marrow transplantation, hematopoiesis Introduction HIV-based lentiviral vectors (LV) were proven to be capable of transducing a broad spectrum of nondividing cells in multiple mammalian species cIAP1 Ligand-Linker Conjugates 14 [1]. (eyesoftheelephants.com)
  • this limitation has been overcome via ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, to produce large quantities of these cells as an attractive source for cellular transplantation [ 16 - 18 ]. (hindawi.com)
  • To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
  • Treatment of complete spinal cord injury patients by autologous bone marrow cell transplantation and administration of granulocyte-macrophage colony stimulating factor. (ui.ac.id)
  • after transplantation to the recipient region, single cells are vulnerable to the interference of various factors, such as ischemia and inflammation, leading to cell loss or death [ 16 ]. (biomedcentral.com)
Ability to differentiate3
Differentiate9
  • Stem cells are very sensitive to environmental stimuli, as external signals in the culture environment provide cues that determine whether stem cells continue to self-renew or whether they differentiate into a specific cell type. (videohall.com)
  • Stem cells from the bone marrow, which is derived from mesoderm, can differentiate into liver, lung, GI tract, and skin, which are derived from endoderm and mesoderm. (wikipedia.org)
  • As these cells move upward out of the crypt, they move along signaling gradients that trigger them to differentiate, giving rise to the mature cell types that populate the villus domain. (stemcell.com)
  • Stem cells have the unique ability to self-renew or to differentiate into various cell types in response to appropriate signals. (sigmaaldrich.com)
  • ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes. (sigmaaldrich.com)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • Remarkably, these mesenchymal-like MUCs expressed prosensory cell markers, which indicated that MUCs were prosensory-like hair cell progenitors that may be able to differentiate into new hair cells. (forgetmenotinitiative.org)
  • Prosensory cells are hair cell progenitors because they differentiate into hair and supporting cells during development (Kelley, 2006). (forgetmenotinitiative.org)
  • They exit quiescent state, proliferate rapidly in response to injury and differentiate into alveolar epithelial type I (AT1) cells to regenerate the damaged alveolar epithelium. (bvsalud.org)
Tissue32
  • Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
  • Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
  • An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
  • Unlike embryonic stem cells, the use of human adult stem cells in research and therapy is not considered to be controversial, as they are derived from adult tissue samples rather than human embryos designated for scientific research. (wikipedia.org)
  • Under normal conditions, tissue stem cells divide slowly and infrequently. (wikipedia.org)
  • The global stem cell banking market growth has gained a major momentum in the recent years, due to the rising awareness of the use of cord blood and cord tissue stem cells in therapeutics, and the extensive application of stem cells in the treatment of autism, lymphoma, anemia, etc. (medgadget.com)
  • Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancerstem cells, developmental studies, genomics and translational research. (firebaseapp.com)
  • Since the introduction of the mouse small intestinal organoid model in 2009, 1 there has been an avalanche of developments in this field, including development of culture conditions for human organoids derived from primary colonic tissue, 2 as well as from human pluripotent stem cells (hPSCs). (stemcell.com)
  • Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
  • Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
  • Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
  • During this process, leukocytes lose lineage-associated markers home and undergo homocytic aggregation, upregulate expression of stem cell antigens, and subsequently redifferentiate to give rise original tissue or, transdifferentiate into a different tissue altogether. (eurekaselect.com)
  • The robustness and efficacy of the retrodifferentiation process in generating unprecedented quantities of stem cells belonging to the three germ layers will enable organ and tissue reconstruction ex vivo, using bio-printing and various scaffold materials. (eurekaselect.com)
  • ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
  • Takahashi and Yamanaka 2006 as well as the lifetime of pluripotent cells in physiological adult tissue (Roy et al. (buyresearchchemicalss.net)
  • MG goes through dramatic tissue development and redecorating during puberty and being pregnant generating not merely luminal epithelial cells but also a distinctive mesenchymal-like epithelial inhabitants specifically basal/myoepithelial cells (Watson and Khaled 2008 Hence MG acts as a perfect system to review the hereditary circuits that control epithelial lineage plasticity. (buyresearchchemicalss.net)
  • However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
  • Embryonic stem cells (cells isolated from the inner cell mass of blastocysts) are pluripotent and can become virtually every tissue of the body. (bio-rad-antibodies.com)
  • Under normal circumstances, turnover of epithelial cell lineages occurs constantly in every 2-7 days but this process become faster during tissue repair and wound healing [ 5 ]. (biomedcentral.com)
  • In contrast, adult stem cells can only give rise to differentiated somatic cells of the particular tissue from which these cells originated (Can/Hematol 2008). (papersowl.com)
  • These adult stem cells are highly specialized and can only produce the tissue in which they reside. (pas.va)
  • In addition, there has been remarkable progress in developing approaches to stimulate these tissue-resident stem cells in situ in the tissue. (pas.va)
  • Biostór / Master Cell Bank is a leading EU-licensed Tissue Establishment (TE) storing life-saving cell therapies, GMP Cell Banks and Clinical Trial samples since 2007. (mastercellbank.com)
  • Serving global markets, it provides cell and tissue biostorage and cold chain logistics solutions to many leading medical and biotechnology research organisations world-wide. (mastercellbank.com)
  • Side-by-side comparison of the biological characteristics of human umbilical cord and adipose tissue-derived mesenchymal stem cells. (ui.ac.id)
  • Cell Tissue Res. (ui.ac.id)
  • Cell Tissue Transplant Ther. (ui.ac.id)
  • 1. Activation of adipose tissue and related mesenchymal stem cells: promotion of anti-inflammatory properties through SOX2 transcription factor and HuR RNA-binding protein modulation. (neurogelenmarche-usa.org)
  • We have suspected that mechanical forces may be of key relevance in adult tissue plasticity as it occurs during embryogenesis, tissue remodeling and mitotic and motile behavior of cells (Vogel et al, 2006). (neurogelenmarche-usa.org)
  • In both processes, rapid proliferation gives rise to new tissue, cell fate has to be specified within that tissue, and distinct positional identities have to be established to generate a properly patterned structure. (frontiersin.org)
  • Tissue-engineered microtissue is an effective strategy for stem cell culture and therapy in nerve tissue engineering. (biomedcentral.com)
  • Here, we identify nonrandom distribution of a pathogenic mtDNA variant across a complex tissue using single-cell RNA-Seq, mitochondrial single-cell ATAC sequencing, and multimodal single-cell sequencing. (jci.org)
ESCs1
  • It is expressed in ESCs, embryonic germ (EG) cells and embryonic carcinoma (EC) cells and essential for early embryonic development [ 14 , 15 ]. (biomedcentral.com)
Somatic and pluripotent cells2
  • RNA sequencing was measured to identify the differential expressed genes due to loss of Sirt6 in somatic and pluripotent cells. (biomedcentral.com)
  • The comparative study of metabolomes of these cells is important for understanding the differences in metabolism between somatic and pluripotent cells, and also the possibl. (shengsci.com)
Pluripotency9
  • Particularly, by performing transcriptome analysis, we observed that several pluripotent transcriptional factors increase in knockout cell line, which explains the underlying loss of pluripotency in Sirt6-null iPS-like cell line. (biomedcentral.com)
  • Differentiated somatic cells can be reprogrammed into a pluripotent-like state through four defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc (OSKM) [ 1 , 2 ]. (biomedcentral.com)
  • Special focus of SCR is on mechanisms of pluripotency and description of newly generated pluripotent stem cell lines. (firebaseapp.com)
  • Octamer-binding transcription factor-4 (Oct4), a transcription factor belongs to the POU transcription factor family Class V, is fundamental for maintaining self-renewal ability and pluripotency of stem cells. (biomedcentral.com)
  • Colony formation assay, sphere-forming ability assay, alkaline phosphatase (AP) activity assay and teratoma-formation assay were used to assess the role of modaline sulfate (MDLS) in promoting self-renewal and reinforcing pluripotency of P19 cells. (biomedcentral.com)
  • These findings demonstrated, for the first time, that MDLS could maintain self-renewal and pluripotency of stem cells. (biomedcentral.com)
  • and from 2002 on pluripotency in embryonic stem cells in the lab of Ali H. Brivanlou. (eurostemcell.org)
  • LIN28 (LIN28A and LIN28B) proteins are highly conserved small cytoplasmic RNA-binding proteins (RBPs) that function as pluripotency factors, regulating the transition from self-renewal to a differentiated cell fate [ 1 ] . (encyclopedia.pub)
  • This pluripotency enables these cells to produce daughter cells of all differentiated somatic cell types, germ cells, and cells of all three embryonic germ layers (Can/Hematol 2008). (papersowl.com)
Biology4
  • In February 2018, the Institute of Integrative Biology collaborated with Anika Therapeutics, Inc., to develop an injectable mesenchymal stem cell (MSC) therapy for the treatment of osteoarthritis. (medgadget.com)
  • Stem Cell Research - Journal - Elsevier Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. (firebaseapp.com)
  • Dr. Heike Kroeger received an undergraduate degree in Biochemistry from Free University Berlin in Germany and earned her PhD in Structural Medicine and Cell Biology at Cambridge University, UK. (nih.gov)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
Characterization2
  • Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture. (sigmaaldrich.com)
  • Mesenchymal stem cells: clinical applications and biological characterization. (ui.ac.id)
Bone14
  • The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
  • Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
  • WO 2016/135723 to the present inventors discloses mammalian bone marrow cells enriched with mitochondria for treatment of mitochondrial diseases. (justia.com)
  • Efficient commitment to functional CD34+ progenitor cells from human bone marrow mesenchymal stem-cell-derived induced pluripotent stem cells. (1library.org)
  • Our data provide evidence that adult stem cells in bone marrow can be efficiently transduced vector administration without preconditioning. (eyesoftheelephants.com)
  • Following intravenous administration of a first-generation LV into adult mice, we found that bone marrow exhibited the highest levels of transgene among nine organs examined, with more than 10% green fluorescent protein-positive (GFP+) cells detected in peripheral blood leukocytes (PBL) in these mice [9]. (eyesoftheelephants.com)
  • It was also observed by others that a significant transgene signal was detected in the bone marrow (BM) by PCR analysis in adult mice of systemic administration of HIV-biased LV [10]. (eyesoftheelephants.com)
  • Bone marrow stem cells from adults have been viewed as the ideal target for gene- and cell-based therapy of genetic diseases, selected malignant diseases, and AIDS. (eyesoftheelephants.com)
  • Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
  • The clinical application cells began in 1956 with the first successful bone marrow transplant, performed between twins, with bone marrow taken from the healthy identical twin, and given to the other, who had leukaemia. (mastercellbank.com)
  • Its clinical introduction in 1986 replaced bone marrow (BM) as a stem cell source to almost 100% in the and to autologous approximately 75% in the allogeneic transplant setting. (mastercellbank.com)
  • Hematopoietic stem cells (HSCs) used in the treatment of cancers and other immune system disorders are found in the red bone marrow in the central part of most bones but principally in the pelvis, femur, and sternum. (mastercellbank.com)
  • Isolation and cultivation of mesenchymal stem cells from iliac crest bone marrow for further cartilage defect management. (ui.ac.id)
  • Functional recovery of spinal cord injury following application of intralesional bone marrow mononuclear cells embedded in polymer scaffold? (ui.ac.id)
Hematopoietic stem1
  • The emergence of innovative products and treatment therapies, such as allogenic cord-blood derived hematopoietic stem cell therapy, is an imperative growth factor for the global market. (medgadget.com)
Embryonic and adult stem1
Murine5
Embryos5
  • Section II is applicable to stem cells derived from human embryos. (firebaseapp.com)
  • Embryonic stem cell research garnered great controversy because it derives cells from human embryos through the process of disassembling the embryo. (firebaseapp.com)
  • This is because, as Robertson has indicated, human embryos consist of one of the most potent sources of stem cells, and the extraction of stem cells from the embryos requires the destruction of the embryos. (firebaseapp.com)
  • Scientists have applied somatic cell nuclear transfer to clone human and mammalian embryos as a means to produce stem cells for laboratory and medical use. (asu.edu)
  • In the cochlea of mouse embryos, it is highly expressed in prosensory cells and down-regulated at the onset of hair cell differentiation. (encyclopedia.pub)
Epithelial cells5
  • We found that adult mouse utricle sensory epithelial cells underwent EMT to become MUCs that were able to propagate in adherent cultures, express mesenchymal markers, and lose the expression of epithelial markers. (forgetmenotinitiative.org)
  • In this study, we maintained mesenchymal-like MUCs in adherent cultures to test new methods that were able to guide MUCs to buy SB-649868 become a number of epithelial cells expressing epithelial genes such as in MUCs. (forgetmenotinitiative.org)
  • The precise mechanism by which Lin28B and let7 miRNA regulate mTORC1 activity in cochlear epithelial cells awaits further investigation. (encyclopedia.pub)
  • Bile acid treatment also increased the expression of KLF5, CDX2, MUC2 and villin in esophageal epithelial cells in a time-dependent manner. (jcancer.org)
  • Moreover, siRNA-mediated knockdown of KLF5 blocked the expression of CDX2, MUC2 and villin, but transfection of a KLF5 expression vector into esophageal epithelial cells promoted their transdifferentiation into columnar-like cells, as demonstrated by increased expression of the intestinal markers CDX2, MUC2 and villin. (jcancer.org)
Human26
Transcriptional2
  • In addition, the authors also review future strategies in DN treatment such as transcriptional reprogramming of mature adult kidney cells into uncommitted induced pluripotent stem cells for renal repair and therapeutics. (emjreviews.com)
  • Accumulating evidence highlights emerging functions of RBPs in the post-transcriptional regulation of inner ear development and hair cell function. (encyclopedia.pub)
Autologous2
  • Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)
  • A few exceptions emerged when successful functional correction was exhibited in children with severe combined immunodeficiency (ADA-SCID and X-SCID) or chronic granulomatous disease following infusion of retrovirally transduced autologous CD34+ cells [12C14]. (eyesoftheelephants.com)
Abstract1
  • The take-home from the abstract is that the data, "demonstrate that mammalian stem cells can protect themselves from some RNA viruses by expressing an alternatively spliced isoform of the enzyme Dicer called aviD, which potentiates antiviral RNA interference. (ipscell.com)
Umbilical cord2
  • Furthermore, MAG Pharmaceuticals, Inc. acquired Cord Blood Registry (CBR) - an umbilical cord blood stem cell collection and storage company - for US$ 700 million in order to expand and diversify its maternal health business in 2015. (medgadget.com)
  • allogenic natural killer cells (NK-cells) generated ex vivo from umbilical cord blood progenitor cells in cancer immunotherapy. (mastercellbank.com)
Lineages5
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • Cells of epithelial lineages can go through phenotypic changes to get mesenchymal features via an epithelial-to-mesenchymal changeover (EMT) plan (Kalluri and Weinberg 2009 Full EMT takes place during mesoderm or neural crest development to generate completely dedicated mesenchymal cell types (Thiery et al. (buyresearchchemicalss.net)
  • In contrast, BMSCs are capable of differentiating into mesenchymal lineages such as osteoblasts, chondrocytes, adipocytes, and even neurons and astrocytes [ 12 ]. (hindawi.com)
  • Three main epithelial cell lineages comprise a crypt: the columnar cells or colonocytes, the mucin-secreting cells or goblet cells, and the enteroendocrine cells. (biomedcentral.com)
  • According to unitarian theory, proposed in 70s, all four cell lineages within the gastrointestinal epithelium are clonal populations derived from a slowly cycling clonogenic stem cells (SC) [ 6 ]. (biomedcentral.com)
Profiles of somatic cells1
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Inducing differentiated somatic cells2
  • A dedifferentiation program was suggested for inducing differentiated somatic cells to become pluripotent embryonic-like stem cells (Halley-Stott et al. (forgetmenotinitiative.org)
  • The methodology included inducing differentiated somatic cells with the primary genes responsible for embryonic stem cell potency. (papersowl.com)
Neurons3
  • Multipotency or multidifferentiative potential is the ability to generate progeny of several distinct cell types, (for example glial cells and neurons) as opposed to unipotency, which is the term for cells that are restricted to producing a single cell type. (wikipedia.org)
  • Stem cell-derived dopamine neurons for brain repair in Parkinson's disease. (mastercellbank.com)
  • 2006). Furthermore, several recently published reports claim that hADSCs are capable under appropriate stimulation of differentiating into other different cell types such as neurons, cardiomyocytes, hepatocytes and pancreatic cells (Schäffler et al. (neurogelenmarche-usa.org)
Therapies4
  • Despite the great promise of stem cells, there are many challenges to overcome before stem cell-derived therapies can be made broadly available, including the lack of processes to manufacture viable and homogenous cell populations. (videohall.com)
  • They are also collaborating with academic researchers in order to expand the potential uses of newborn stem cell therapies that may be available to patients and their families. (medgadget.com)
  • Stem cell therapies, once a dream, are now becoming a reality. (pas.va)
  • We propose an opportunity to target the cancer cell/microenvironment interface instead of the Notch pathway itself in the development of cancer therapies. (5dok.org)
Functional5
  • Oct4-induced oligodendrocyte progenitor cells enhance functional recovery in spinal cord injury model. (biodonostia.org)
  • These organoids include a polarized epithelial layer that surrounds a functional lumen and contains cell types of the intestinal epithelium present in proportions and relative spatial arrangement that mimic what is observed in vivo. (stemcell.com)
  • Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
  • He co-ordinates the EU Horizon2020 funded stem cell research consortium - INTENS which aims to aims to make a functional reconstructed bowel for people with Short Bowel Syndrome. (eurostemcell.org)
  • Cord blood is now being enriched with functional stem and progenitor cells and also immune modulatory cells. (mastercellbank.com)
Adherent1
  • Current bioprocesses also require that stem cells, which are normally adherent, be cultured in suspension in order to achieve higher cell densities. (videohall.com)
20173
  • 2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
  • According to the Leukemia and Lymphoma Society, an estimated 172,910 people in the U.S. were diagnosed with leukemia, lymphoma or myeloma in 2017, which consequently led to the higher adoption of stem cells for efficient treatment. (medgadget.com)
  • Aug 23, 2017 · To start a research paper on stem cells, students have to know the basics about them first and narrow down the general topic from there. (firebaseapp.com)
Intestinal5
  • The intestinal epithelium incorporates several distinct cell populations, including the rapidly dividing intestinal stem cells (ISCs) that facilitate the typical four-to-five day turnover cycle of the adult intestinal epithelium. (stemcell.com)
  • Diagram of the small intestinal epithelium highlighting the identity and spatial arrangement of key epithelial cell types. (stemcell.com)
  • The adult intestinal epithelium is primarily composed of six cell types that are arranged in a crypt-villus structure (Figure 1). (stemcell.com)
  • 18 At the base of the intestinal crypt, the ISCs are found intercalated with Paneth cells, 17 credited with much of the signaling required to maintain the stem cell niche. (stemcell.com)
  • The adult mammalian intestinal epithelium is unique for its rapidly self renewing property. (biomedcentral.com)
Organoids2
  • We also provide an in-depth protocol for the generation of head and neck squamous cell carcinoma organoids and their subsequent use in semi-automated therapy screens. (nature.com)
  • Kretzschmar, K. & Clevers, H. Organoids: modeling development and the stem cell niche in a dish. (nature.com)
Pancreatic cells1
  • 2007). Such a dedifferentiation program occurs in mammalian pancreatic epithelial cell cultures, in which pancreatic cells dedifferentiate into mesenchymal-like cells that are able to re-enter the cell cycle, probably via epithelial-to-mesenchymal transition (EMT) (Gallo et al. (forgetmenotinitiative.org)
Niche5
  • The niche the stem cell is found in plays a large role in maintaining quiescence. (wikipedia.org)
  • Perturbed niches cause the stem cell to begin actively dividing again to replace lost or damaged cells until the niche is restored. (wikipedia.org)
  • I hadn't really thought previously of apoptotic cells potentially serve as part of the niche. (ipscell.com)
  • Because of their specific niche and a limited number of stem cells, the key question is whether there are different anti-viral mechanisms against viral infection notably COVID-19. (shengsci.com)
  • During the process of development, neural crest cells migrate out from their niche between the newly formed ectoderm and the neural tube. (shengsci.com)
Progenitors3
  • Progenitors can go through several rounds of cell division before finally differentiating into a mature cell. (wikipedia.org)
  • Shi and Advantage 2013), and locks cell progenitors possess been determined in the adult mammalian utricle and cochlea (Li et al. (forgetmenotinitiative.org)
  • 2007). Some research possess recommended that assisting cells had been the most likely resource of progenitors (Kelley et al. (forgetmenotinitiative.org)
Precursor cells1
  • Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
Donor3
  • Nuclear transfer efficiency is enhanced by introduction of compatible cytoplasm or mitochondrial DNA (same species or similar to donor cell or nucleus). (justia.com)
  • However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
  • A new cell therapy, donor lymphocyte infusion, emerged in the 1990s to ensure that the donor stem cells remain engrafted whereby lymphocytes from the blood of a donor are given to a patient who has already received a hematopoietic stem cells from the same donor. (mastercellbank.com)
Endothelial Cells2
Therapy4
  • For instance, in 2011, the FDA approved Hemacord, which is the first FDA-licensed hematopoietic progenitor cells-cord (HPC-C) cell therapy manufactured by New York Blood Center, Inc. Furthermore, in 2014, Hemacord was conferred the Best Biotechnology Product Award by Prix Galien USA. (medgadget.com)
  • The potential of CAR T cell therapy for prostate cancer , Nat Rev Urology. (ipscell.com)
  • Peripheral blood stem cell transplantations (PBSCT) are now the most common cell therapy procedure. (mastercellbank.com)
  • The cell therapy industry is rapidly expanding. (mastercellbank.com)
Proliferation and differentiation3
  • The ovarian microenvironment affects the proliferation and differentiation of OGSCs, and immune cells and related cytokines are important components of the microenvironment. (biomedcentral.com)
  • It is worth noting that the proliferation and differentiation of stem cells cannot be separated from the surrounding microenvironment, and immune system related cells are an important part of the microenvironment of the OGSCs nest [ 14 ]. (biomedcentral.com)
  • Depletion of CD8 T cells or neutralization of cytokine IFN-γ using anti-CD8 or anti-IFN-γ monoclonal antibody significantly reduced AT2 cell proliferation and differentiation into AT1 cells in mice after bacterial pneumonia. (bvsalud.org)
Isolation3
  • Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation. (sigmaaldrich.com)
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
  • 4472942). For mitochondria transfer generation: Mitochondria were harvested from HEK293T DsRed cells, LP351 PBMC, or LP298 PBMC using a Qproteome Mitochondria Isolation Kit (Qiagen, Cat. (nih.gov)
Regulate4
  • In hematopoietic stem cells, the MAPK/ERK pathway and PI3K/AKT/mTOR pathway regulate this transition. (wikipedia.org)
  • The ability to regulate the cell cycle in response to external cues helps prevent stem cell exhaustion or the gradual loss of stem cells following an altered balance between dormant and active states. (wikipedia.org)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • They can regulate stem cell functions by modulating effects of Wnt signalling on ephrin B1 (EFNB1) and its receptors EPHB2, EPHB3 in the stem cells [ 4 ]. (biomedcentral.com)
Types14
  • The first of which is their ability to divide or self-renew indefinitely, and the second their ability to generate all the cell types of the organ from which they originate, potentially regenerating the entire organ from a few cells. (wikipedia.org)
  • To ensure self-renewal, stem cells undergo two types of cell division (see Stem cell division and differentiation diagram). (wikipedia.org)
  • Among source types, the cord blood segment holds the leading stance in the global stem cell banking market, owing to the ability of cord blood stem cells to treat over 80 blood-related diseases such as sickle cell anemia and thalassemia and various types of cancers such as leukemia and myeloma. (medgadget.com)
  • This research is focused on the two mammalian stem cell types, namely, the embryonic stem cells and adult (non-embryonic) stems cells. (firebaseapp.com)
  • In any case, the respective cell types may then be selectively cultured to enrich their percentage population to eventually obtain a single cell type and culture. (justia.com)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • Thereafter, they give rise not only to ectodermal cell types, but also to mesodermal cell types. (shengsci.com)
  • Stem cells are unspecialized cells that can develop into several different cell types. (asu.edu)
  • Pluripotent stem cells are a special cell type that can give rise to other types of cells and are essential for development. (asu.edu)
  • Specific stem cell types possess unique potencies, or abilities to self-renew. (papersowl.com)
  • That is to say, one type of stem cell may be capable of differentiating into all adult cell types, while another may only be capable of maturing into one specific somatic cell type. (papersowl.com)
  • Hematopoietic stem cells, which can produce several different types of blood cells, are prime examples of multipotency. (papersowl.com)
  • The term stem cells dates back to the late 1800s at which time it referred to the fertilized egg, a pluripotent cell able to give rise to all of the complex cell types that comprise our body. (pas.va)
  • G allele was neither evenly nor randomly distributed across diverse cell types. (jci.org)
Germ5
  • In recent years, the discovery of ovarian germ stem cells (OGSCs) has provided a new research direction for the treatment of female infertility. (biomedcentral.com)
  • It was found that ovoid cells in the epithelial layer of the ovary simultaneously expressed the germ cell specific marker MVH and the proliferation of cell marker BrdU using double-immunofluorescence staining. (biomedcentral.com)
  • This discovery invalidated the "fixation theory of primordial follicle pool" and suggested for the first time the hypothesis that germ stem cells also exist in the ovary. (biomedcentral.com)
  • Niikura [ 5 ] and others observed germ cells expressing Stra8 (stimulated by retinoic acid gene 8) in the ovarian surface epithelium, indicating that there may be germ cells with meiotic function in the ovary. (biomedcentral.com)
  • Somatic cells are cells that have gone through the differentiation process and are not germ cells. (asu.edu)
Differentiation of stem cells3
  • The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
  • Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
  • Pancreatic Differentiation of Stem Cells Reveals Pathogenesis of a Syndrome of Ketosis-Prone Diabetes. (reprocell.com)