Mesenchymal stem cells into cardiomyocytes. Medical search. Frequent questions

The key attributes like isolation efficiency, cell yield, colony-forming unit-fibroblast (CFU-F) frequency, phenotypic characteristics, and multi-lineage differentiation capacity were determined for the isolated MSCs from bone marrow specimens. (journalcra.com)
Further, when those MSCs were analyzed for their specific marker expression using flowcytometry, they were found to be positive for CD29, CD73, CD90, and CD105 and negative for CD34 and CD45 stem cell markers respectively. (journalcra.com)
Apart from this, it was observed that those MSCs, when induced to differentiate into cardiomyocytes, they successfully differentiated into cardiomyocyte like cells with flat, wide, myotube like structure with sarcomeric organization, which were later confirmed to be cardiomyocytes by qRT-PCR. (journalcra.com)
Thus, this study shows that MSCs were isolated from human bone-marrow and subsequently differentiated into cardiomyocytes with 100% success rate. (journalcra.com)
Mesenchymal stem cells (MSCs) also known as mesenchymal stromal cells or medicinal signaling cells are multipotent stromal cells that can differentiate into a variety of cell types, including osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells) and adipocytes (fat cells which give rise to marrow adipose tissue). (wikipedia.org)
While the terms mesenchymal stem cell (MSC) and marrow stromal cell have been used interchangeably for many years, neither term is sufficiently descriptive: Mesenchyme is embryonic connective tissue that is derived from the mesoderm and that differentiates into hematopoietic and connective tissue, whereas MSCs do not differentiate into hematopoietic cells. (wikipedia.org)
Mesenchymal stem cells (MSCs), a term first coined by Arnold I. Caplan in 1991, are characterized morphologically by a small cell body with a few cell processes that are long and thin. (wikipedia.org)
However, MSCs are found in much higher concentration in the Wharton's jelly compared to cord blood, which is a rich source of hematopoietic stem cells. (wikipedia.org)
These cells have several advantages over bone-marrow-derived MSCs. (wikipedia.org)
MSCs have been seen to even differentiate into neuron-like cells, but doubt remains about whether the MSC-derived neurons are functional. (wikipedia.org)
Mesenchymal Stem Cells (MSCs) are a heterogeneous subset of stromal stem cells that originate from the mesoderm layer of the fetus. (tocris.com)
Mesenchymal stem cells (MSCs) have been widely used in regenerative medicine and clinical therapy due to their capabilities of proliferation, differentiation, and immune regulation. (techscience.com)
The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
Cellular therapy based on mesenchymal stem cells (MSCs) is a promising novel therapeutic strategy for the osteonecrosis of the femoral head (ONFH), which is gradually becoming popular, particularly for early-stage ONFH. (biomedcentral.com)
Nonetheless, the MSC-based therapy is challenging due to certain limitations, such as limited self-renewal capability of cells, availability of donor MSCs, and the costs involved in donor screening. (biomedcentral.com)
As an alternative approach, MSCs derived from induced pluripotent stem cells (iPSCs), which may lead to further standardized-cell preparations. (biomedcentral.com)
We proposed that hypoxic preconditioning could increase survival of bone marrow mesenchymal stem cells (MSCs) via upregulation of Pim-1 and aimed to determine the microRNAs that modulate the expression of Pim-1. (biomedcentral.com)
In addition, gain and loss-of-function studies with miR-206 mimics and inhibitors showed that inhibition of miR-206 in hypoxic MSCs promoted the migration ability of the cells, prevented cell apoptosis, and protected membrane potential of mitochondria, while the benefits were all blocked by Pim-1 inhibitor. (biomedcentral.com)
Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (stemcellslab.net)
Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (stemcellslab.net)
Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (stemcellslab.net)
The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (stemcellslab.net)
and signals from the injected cells were found early after administration of the MSCs at the highest frequencies in the lungs, followed by liver and spleen. (stemcellslab.net)
In particular, stem cells, including mesenchymal stem cells (MSCs), hematopoietic stem cells (HSCs), and induced pluripotent stem cells (iPSCs), and immune cells, such as T-cells and Natural Killer (NK) cells, have been favored candidates for regenerative medicine and cell-based cancer immunotherapy, respectively. (biomedcentral.com)
The developmental story of cell-based cancer immunotherapy is not so different from MSCs in regenerative medicine. (biomedcentral.com)
Among the affected cells are mesenchymal stem cells (MSCs), which are involved in the maintenance and repair of tissues in the body. (edu.au)
However, MSCs also have been associated with resistance of tumour cells to drugs including doxorubicin. (edu.au)
RESULTS: In vitro data demonstrated improved cardiomyocyte mitochondrial respiration upon coculture with MSCs that was blunted when adding the exosomal antagonist GW4869. (bvsalud.org)
Bone marrow derivedmesenchymal stem cells (MSCs) are a promising approach to the treatment of cardiac injury after myocardial infarction (MI). (biomedcentral.com)
However, little is known about the underlying mechanisms and the fate of the transplanted cells.Our aim was to in vivo monitoring the magnetically labeled MSCs after transplantation into infarcted rat hearts and determining the effects on cardiac function using a 7.0 T magnetic resonance imaging (MRI) scanner. (biomedcentral.com)
Seven days after MI, rats (syngeneic females) were randomized to injections of labeled MSCs (2×10 6 cells/50μL) or saline (50μL) into the border zone of infarcted myocardium. (biomedcentral.com)
However, MSCs attenuate left ventricular dilatation and dysfunction after MI, which may attribute to enhanced angiogenesis, inhibition of host cell apoptosis and fibrosis. (biomedcentral.com)
The ERK-MAPK signaling pathway plays a role in the differentiation of mesenchymal stem cells (MSCs) into adipocytes [1] [2] . (cusabio.com)
p38 pathway plays a very important role in the osteogenic differentiation of mesenchymal stem cells (MSCs). (cusabio.com)
Mesenchymal stem cells (MSCs) can be readily isolated from a number of adult and fetal tissues, and have the capacity of expansion in vitro on a clinical scale. (tmu.edu.tw)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids. (tocris.com)
There are several culture systems for generation and expansion of pluripotent stem cells. (cellculturedish.com)
Human pluripotent stem cells (hPSCs) have the ability to generate all cell types in the human body and can be used in many applications in basic research and translational medicine, including disease modeling, drug screening and cell therapy. (stemcell.com)
Proteomic Analysis of Exosomes during Cardiogenic Differentiation of Human Pluripotent Stem Cells. (plurisomes.com)
Mining Exosomal MicroRNAs from Human-Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration. (plurisomes.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)

It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
I am reprogramming hematopoietic stem cells to iPSCs. (cellculturedish.com)
For example, hematopoietic stem cells differentiate into various immune cells. (cusabio.com)
2016). GSK3 Deficiencies in Hematopoietic Stem Cells Initiate Pre-neoplastic State that Is Predictive of Clinical Outcomes of Human Acute Leukemia. (connsante.com)

They include endothelial cells, fibroblasts, and keratinocytes. (frontiersin.org)
Peritoneal cavity is rich in number of cells like mesothelial cells, fibroblasts, endothelial cells, adipocytes and immune cells like macrophages, T cells, B cells, mast cells. (ukessays.com)
2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
The bone morrow contains several stem cell types including hematopoetic stem cells, endothelial progenitor cells, mesenchymal (stromal) stem cells and multipotent adult progenitor cells. (escardio.org)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)

Progenitor cell therapy describes the use of multipotent cells of various cell lineages (autologous or allogeneic) for tissue repair and/or regeneration. (southcarolinablues.com)
Progenitor cell therapy is being investigated for the treatment of damaged myocardium resulting from acute or chronic cardiac ischemia and for refractory angina. (southcarolinablues.com)
For individuals who have acute cardiac ischemia who receive progenitor cell therapy, the evidence includes 2 randomized controlled trials (RCTs) with 200 patients, numerous small RCTs, and meta-analyses of these RCTs. (southcarolinablues.com)
Overall, this evidence has suggested that progenitor cell treatment may be a promising intervention, but robust data on clinical outcomes are lacking. (southcarolinablues.com)
For individuals who have chronic cardiac ischemia who receive progenitor cell therapy, the evidence includes a nonrandomized comparative trial and systematic reviews of smaller RCTs. (southcarolinablues.com)
Results of the nonrandomized trial are encouraging, because this is the first controlled trial that has reported a significant mortality benefit for progenitor cell treatment. (southcarolinablues.com)
For individuals who have refractory angina who receive progenitor cell therapy, the evidence includes phase 2 trials and a phase 3 pivotal trial. (southcarolinablues.com)
Additional larger trials are needed to determine whether progenitor cell therapy improves health outcomes in patients with refractory angina. (southcarolinablues.com)
1 Treatment with progenitor cells (i.e., stem cells) offers potential benefits beyond those of standard medical care, including the potential for repair and/or regeneration of damaged myocardium. (southcarolinablues.com)
Overexpression of Pim-1 in stem/progenitor cells stimulated cell cycling and enhanced cardiac regeneration in vivo. (biomedcentral.com)
9 ] reported that hypoxic preconditioning increases survival of cardiac progenitor cells via upregulation of Pim-1, discovering a method for nongenetic modification of Pim-1 in stem cells. (biomedcentral.com)
Although there is no definitive evidence, combinations of progenitor cells seem to be more benefical than specific stem cell type for cardiac repair (3). (escardio.org)
Interestingly, contradictory data exist for transformation of bone marrow derived progenitor cells (BMPC) into new cardiomyocytes and alternative mechanisms as enhanced neovascularization, enhanced scar tissue formation due to augmented inflammatory response and decreased apoptosis have been suggested for the benefical effects of these cells on myocardial function after myocardial infarction (4). (escardio.org)
Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids. (stembook.org)

It has been thought that functional myocardial repair and regeneration could be regulated by stem cell-cardiomyocyte contact. (syr.edu)
The biochips will help us gain knowledge of contact-mediated interactions between stem cells and cardiomyocytes, which are fundamental for formulating a strategy to achieve stem cell-based cardiac tissue regeneration. (syr.edu)
Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
However, genetic modification of stem cells is not the first promising option in their utilization for tissue regeneration. (biomedcentral.com)
The idea of myocardial regeneration with stem cell transplantation after myocardial infarction receives tremendous interest. (escardio.org)
Molecular cardiology, cell biology, cardiac regeneration. (z2hospital.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)

Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
Embryonic stem cell-derived exosomes inhibit doxorubicin-induced TLR4-NLRP3-mediated cell death-pyroptosis. (plurisomes.com)
Generation of -Knockout Human Embryonic Stem Cell Lines to Model Autophagic Dysfunction and Exosome Secretion in Fabry Disease-Associated Hypertrophic Cardiomyopathy. (plurisomes.com)
Comparison of Non-Coding RNAs in Exosomes and Functional Efficacy of Human Embryonic Stem Cell- versus Induced Pluripotent Stem Cell-Derived Cardiomyocytes. (plurisomes.com)
Exosomes Derived from Embryonic Stem Cells as Potential Treatment for Cardiovascular Diseases. (plurisomes.com)
For example, humans are derived from fertilized eggs, and this process involves the differentiation of embryonic stem cells. (cusabio.com)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
Embryonic stem cells are stem cells derived from the inner cell mass of an early, preimplantation stage embryo known as a blastocyst. (bvsalud.org)

The standard test to confirm multipotency is differentiation of the cells into osteoblasts, adipocytes and chondrocytes as well as myocytes. (wikipedia.org)
They can differentiate into cells of the mesodermal lineage, such as adipocytes, and osteocytes. (tocris.com)

The term encompasses multipotent cells derived from other non-marrow tissues, such as placenta, umbilical cord blood, adipose tissue, adult muscle, corneal stroma, or the dental pulp of deciduous (baby) teeth. (wikipedia.org)
Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
Allogenic Umbilical Cord-Derived Mesenchymal Stromal Cells Sustain Long-Term Therapeutic Efficacy Compared With Low-Dose Interleukin-2 in Systemic Lupus Erythematosu. (stembook.org)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)

Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation. (edu.au)
GW4869, an exosomal release antagonist, was used to determine the effect of mesenchymal stem cells-derived exosomal signaling on cardiomyocyte recovery. (bvsalud.org)

These stem cells are capable of differentiating into chondrocytes, cardiomyocytes, melanocytes, and hepatocyte‐like cells in vitro. (wikipedia.org)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
Maintenance of high quality hPSCs is dependent on consistent in vitro cell culture conditions and handling techniques. (stemcell.com)
METHODS: In vitro methods involved exposing H9C2 cardiomyocytes to hypoxia followed by normoxic coculture with porcine mesenchymal stem cells. (bvsalud.org)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)

With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
Due to their secretory functions, differentiation capabilities, specific homing effects through chemotaxis, distinctive therapeutic potentials, and ex vivo expandability, cells have become an attractive reagent for advanced therapeutic strategies. (biomedcentral.com)
Therefore, the ability to modify cells and manipulate their functions according to intended therapeutic designs has been the central scientific interest in the field of biomedical research. (biomedcentral.com)
Cell surface engineering to provide new characteristics and functions to cells has drawn continuous interest from researchers in biomedical science as cell therapy has emerged as a prominent therapeutic strategy equivalent and complementary to the conventional therapeutic approaches. (biomedcentral.com)
Unfortunately, these breakthrough discoveries in both regenerative medicine and cancer immunotherapy using cells as therapeutic reagents soon faced a common problem: the inability to control cellular functions to maximize the therapeutic benefits. (biomedcentral.com)
As such, stem cell therapy cannot be regarded as a valid therapeutic option for patients with cardiovascular disease in the present era of evidenced-based medicine, yet it holds great hope for the future. (escardio.org)
Zhao Y, Zhu J, Zhang N, Liu Q, Wang Y, Hu X, Chen J , Zhu W, Yu H. GDF11 enhances therapeutic efficacy of mesenchymal stem cells for myocardial infarction via YME1L-mediated OPA1 processing. (z2hospital.com)
Cardiac Fibroblast-Induced Pluripotent Stem Cell-Derived Exosomes as a Potential Therapeutic Mean for Heart Failure. (plurisomes.com)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)

These findings indicated that cell transplantation therapy for the patients with heart failure might possibly be achieved using the regenerated cardiomyocytes from autologous bone marrow cells in the near future. (elsevierpure.com)
In these studies, however, transplanted cells were followed, if at all, at the site of transplantation, and biodistribution was not an issue. (stemcellslab.net)
This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (stemcellslab.net)
Several preliminary reports have demonstrated that local stem cell transplantation in patients with acute myocardial infarction is safe and may lead to improved myocardial function and perfusion. (escardio.org)
In experimental and clinical studies unselected mononuclear bone marrow cells as well as specific subpopulations have been used for transplantation. (escardio.org)
MRI was used to evaluate stem cell migration, signal intensity changes and cardiac function at baseline (1 day before transplantation), 3 days, 2 weeks and 4 weeks after transplantation, respectively. (biomedcentral.com)

We have isolated a cardiomyogenic cell line (CMG cell) from murine bone marrow mesenchymal stem cells. (elsevierpure.com)
Distinct roles of glycogen synthase kinase (GSK)-3alpha and GSK-3beta in mediating cardiomyocyte differentiation in murine bone marrow-derived mesenchymal stem cells. (connsante.com)

Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
However, in spite of their resistance to hypoxia only a small portion of cells survive when transplanted into an infarct scar (6). (escardio.org)
RNA sequencing identified 8 differentially expressed micro RNAs in normoxia vs hypoxia-induced exosomes that may modulate the expression of key mitochondrial (peroxisome proliferator-activator receptor gamma coactivator 1-alpha and adenosine triphosphate synthase) and inflammatory mediators (nuclear factor kappa-light-chain enhancer of activated B cells, interferon gamma, and interleukin 1ß). (bvsalud.org)

Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)

abstract = "Understanding how stem cells interact with cardiomyocytes is crucial for cell-based therapies to restore the cardiomyocyte loss that occurs during myocardial infarction and other cardiac diseases. (syr.edu)

International Journal of Biochemistry and Cell Biology. (edu.au)
He has taught anatomy, physiology, and cell biology at Trinity Christian College for 34 years, and he still teaches there in addition to his work at Argonne. (anl.gov)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)

Bone marrow mesenchymal stem cells secrete factors that exert anti-apoptotic and pro-apoptotic effects on H9c2 cardiac myoblasts. (anl.gov)
Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
In this study we asked if MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if artificial cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)

We are working with mesenchymal stem cells and need to measure their ability to suppress T-cell proliferation. (cellculturedish.com)
We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
MAPK can cause the activation of transcription factors in the nucleus, participate in the process of signal transmission from the cell surface to the nucleus, and regulate cell proliferation and differentiation. (cusabio.com)
This pathway can also affect the proliferation and differentiation of red blood cells. (cusabio.com)

Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)

Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)
In this study, a collagen patch embedded with mesenchymal stem cell (MSC)-derived exosomes was developed, which can be surgically applied to the epicardial surface distal to LIMA anastomosis. (bvsalud.org)
Exosomes secreted by hiPSC-derived cardiac cells improve recovery from myocardial infarction in swine. (plurisomes.com)
Exosomes are cell-derived vesicles that are present in many and perhaps all biological fluids, including blood, urine, and cultured medium of cell cultures. (exosome-rna.com)
Exosomes contain various molecular constituents of their cell of origin, including proteins and RNA. (exosome-rna.com)

Chen, X. Mesenchymal stem cells improve cardiac function after myocardial infarction in rats without long-term survival: a serial 7.0T MRI study. (biomedcentral.com)

Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)

iCell Cardiomyocytes are human heart cells derived from iPSCs. (pharmaceutical-technology.com)
It is not clear if the cardiomyocytes referred to in your question are from a primary source or derived from iPSCs. (cellculturedish.com)

Cellular Dynamics International (CDI) has raised $40.6m to increase the production capacity for its iCell Cardiomyocytes and to initiate additional human tissue cell products for biomedical and pharmaceutical drug development and safety research. (pharmaceutical-technology.com)
This product line is the first commercial product based on stem cells grown from adult tissue using iPSC technology to help pharmaceutical companies speed up the discovery of safer, more effective drugs. (pharmaceutical-technology.com)
Stromal cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside. (wikipedia.org)
Peritoneum is defined as gliding membrane, composed of mesothelial cells maintained by basement membrane and thin layer of connective tissue. (ukessays.com)
Currently we prep the tissue sample, plate cells and grown them in selection media. (cellculturedish.com)
One great advantage of MSC is that these cells may be directly obtained from individual patients, thereby eliminating the complications associated with immune rejection of allogenic tissue and infectious diseases. (biomedcentral.com)
Cell-based therapy has expanded its influence in cancer immunotherapy, regenerative medicine, and tissue engineering. (biomedcentral.com)
Repair of scar tissue constitutes a challenge for cardiac stem cell therapy due to lack of adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)

Bone marrow mesenchymal stem cells (BMSCs) have been reported to attenuate myocardial I/R injury via their paracrine effects, which can be enhanced by hypoxic preconditioning. (researchsquare.com)
The administration of bone marrow-derived mesenchymal stem cells (BMSCs) is emerging as a promising approach for I/R injury-induced myocardial infarction due to its multiple differentiation potential(7, 8). (researchsquare.com)

Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)

Dr. Novas holds key positions in prestigious organizations related to regenerative medicine and stem cell research, including the Gene-Therapy Biomedical Research College in Wellington, Florida, and the Far Eastern University in Manila, Philippines. (regenestem.com)

Cancer Cell Int. 2022;22(1):330. (stembook.org)

Mesenchymal stem cells secrete factors that inhibit apoptosis in H9c2 cardiac myoblasts. (anl.gov)
These include bone marrow-derived cells, peripheral blood derived stem cells and skeletal myoblasts (3). (escardio.org)
The resistant state of skeletal myoblasts to ischemia renders these cells suitable candidates for repair of chronically infracted and failing heart (5). (escardio.org)
Myoblasts retain skeletal muscle properties and couple only sporadically to resident cardiomyocytes precluding synchronized contraction as mechanism of benefit (8). (escardio.org)
Moreover, it seems that paracrine action of skeletal myoblasts facilitate neighboring cardiomyocytes to maintain their replicative potential and/or stimulate differentiation of native cardiac stem cells (8,9). (escardio.org)

Stem cells have potential as a source of cells and tissues for research and treatment of disease. (tocris.com)
Built upon scDiffCom, scAgeCom is an atlas of age-related cell-cell communication changes covering 23 mouse tissues from 58 single-cell RNA sequencing datasets from Tabula Muris Senis and the Calico murine aging cell atlas. (nature.com)
RT-PCR provides the possibility to assess gene transcription in cells or tissues. (honestkfood.com)
All tissues originate from stem cells. (bvsalud.org)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)

the expression levels of protein associated with apoptosis and endoplasmic reticulum stress (ERS) of SKO-007 cells in various groups were detected by Western blotting method. (researchgate.net)
Objective: To investigate the effects of prion protein 106-126 peptide on inducing apoptosis in differentiated PC12 cells. (researchgate.net)
Conclusion: Prion protein 106-126 peptide can induce apoptosis in differentiated PC12 cells and present cellular toxicity definitely. (researchgate.net)
These protective effects may be associated with the inhibition of apoptosis and inflammation, as reflected by less TUNEL-positive cells and lower levels of serum proinflammatory cytokines (Interleukin-1β, Interleukin-6, tumor necrosis factor-α) and cardiac troponin I in the N-CM group compared with the vehicle group. (researchsquare.com)
Also, overexpression of Pim-1 inhibited cardiomyocyte apoptosis and protected mice from infarction injury, which revealed the potential cardioprotective role of Pim-1. (biomedcentral.com)
In addition, MSC-treated hearts had significantly increased capillary density in the peri-infarct region, and lower cardiomyocytes apoptosis and collagen deposition. (biomedcentral.com)
In the present study we found that As 2 O 3 induced ROS production and apoptosis in glioma cells. (biomedcentral.com)
Furthermore, apoptosis and cytotoxicity induced by As 2 O 3 in glioma cells were decreased after silencing the mitoferrin-2 gene. (biomedcentral.com)

They are sometimes referred to as bone marrow stromal stem cells. (wikipedia.org)

At the beginning of 2020, STEMCELL Technologies conducted a survey asking scientists to help highlight the needs and challenges in the hPSC field and to gather ideas on how to achieve greater reproducibility. (stemcell.com)
2020). Editorial Expression of Concern: Implantation of a Novel Allogeneic Mesenchymal Precursor Cell Type in Patients with Ischemic Cardiomyopathy Undergoing Coronary Artery Bypass Grafting: an Open Label Phase IIa Trial. (edu.gr)

OBJECTIVE: This study aimed to investigate whether or not the application of a stem cell-derived exosome-laden collagen patch (EXP) during coronary artery bypass grafting (CABG) can recover cardiac function by modulating mitochondrial bioenergetics and myocardial inflammation in hibernating myocardium (HIB), which is defined as myocardium with reduced blood flow and function that retains viability and variable contractile reserve. (bvsalud.org)

The isoform of contractile protein genes indicated that their muscle phenotype was similar to fetal ventricular cardiomyocytes. (elsevierpure.com)

Mesothelial cells play a vital role in maintaining the structure of peritoneal membrane and peritoneal homeostasis. (ukessays.com)
Bioincompatible dialysis fluid causes mesothelial cells to undergo uncontrolled mesothelial-to-mesenchymal transition triggered by Transforming growth factor-Î²1, which leads morphological and functional disruption of membrane. (ukessays.com)
In this designed project, we are going to focus on mesothelial cells along with Transforming growth factor-Î² (TGF-Î²1), which is considered as main mediator of organ fibrosis development. (ukessays.com)
check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)

Potentials of combining nanomaterials and stem cell therapy in myocardial repair. (plurisomes.com)

The p38 signaling pathway regulates the differentiation direction of stem cells, which may be related to the regulation of the stability of p53 protein [3] [4] . (cusabio.com)

The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. (frontiersin.org)
The exosome vesicles are a type of extracellular vesicles (EV), which are defined as lipid-bilayer spheroid structures, without replicating capacity, that are released from cells, including both prokaryotes and eukaryotes. (frontiersin.org)
Targeted Therapy for Peri-prosthetic Osteolysis Using Macrophage Membrane-encapsulated Human Urine-derived Stem Cell Extracellular Vesicles. (stembook.org)

Base editing correction of hypertrophic cardiomyopathy in human cardiomyocytes and humanized mice. (stembook.org)

Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)

The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)

Intravenous administration of mesenchymal stem cells ( MSC ) following myocardial infarction prevents contractile dysfunction. (anl.gov)

These bone marrow stem cells do not contribute to the formation of blood cells, and so do not express the hematopoietic stem cell marker CD34. (wikipedia.org)
The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)

This literature review is about mechanism of mesothelial -mesenchymal transition (MMT), myofibroblasts and its origin hypothesis, clock genes and their cross relation with organ fibrosis. (ukessays.com)
Human glioma cell lines were used to explore the mechanism of As 2 O 3 's antitumor effects. (biomedcentral.com)

Pseudotime analysis showed that CFs differentiated into two distinct cell fates, one of which produced activated myofibroblasts, and the other which produced protective cells that were associated with reduced fibrosis levels, increased antioxidative stress responses, and the ability to promote angiogenesis. (hindawi.com)
Finally, we found that transcription factors Jun , Fos , Atf3 , and Egr1 were upregulated in protective cells, especially Egr1 was predicted to be involved in the regulation of genes related to antioxidant stress and angiogenesis, suggesting a role in promoting differentiation into this cell phenotype. (hindawi.com)

Anatomy50

Organisms16

Diseases6

Chemicals and Drugs72

Analytical, Diagnostic and Therapeutic Techniques and Equipment55

Phenomena and Processes57

Disciplines and Occupations3

Technology, Industry, Agriculture12

MSCs37

Pluripotent stem6

Hematopoietic stem4

Endothelial6

Progenitor14

Regeneration8

Embryonic12

Adipocytes2

Umbilical cord5

Effect of mesenchymal2

Vitro5

Therapeutic12

Transplantation6

Murine bone2

Hypoxia3

Osteoblasts1

Abstract1

Biology3

Exert3

Proliferation5

Autologous bone1

Exosomes5

Function after myocardial infarction1

Adipose tissue1

Derived from iPSCs2

Tissue10

BMSCs2

Induced to differentiate1

Regenerative medicine1

20221

Myoblasts5

Tissues5

Apoptosis8

Stromal stem cells1

20202

Cardiac Function1

Fetal1

Mesothelial cells4

Myocardial repair1

Regulates the differentiation1

Extracellular vesicles3

Hypertrophic Cardiomyopathy1

Molecular1

Differentiation of stem cells2

Intravenous1

Bone marrow stem cells2

Mechanism2

Angiogenesis2