Mesenchymal stem cells for hematopoietic stem cell niche. Medical search. Frequent questions

Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)
MSCs naturally reside in a specialized niche in vivo , which mainly consists of the extracellular matrix (ECM). (hindawi.com)
We show that marrow from human NHOs contains hematopoietic stem cell (HSC) niches, in which mesenchymal stromal cells (MSCs) and endothelial cells provide an environment supporting HSC maintenance, proliferation, and differentiation. (jci.org)
The MSCs are multipotent cells that are able to differentiate into the mesenchymal lineage cells, which include osteoblasts, adipocytes, chondrocytes, fibroblasts, and other stromal cells [ 11 ] [ 12 ] . (encyclopedia.pub)
The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
Agrin is expressed by multipotent nonhematopoietic mesenchymal stem cells (MSCs) and by differentiated osteoblasts lining the endosteal bone surface, whereas Lin−Sca1+c-Kit+ (LSK) cells express the α-dystroglycan receptor for agrin. (ox.ac.uk)
In vitro, agrin-deficient MSCs were less efficient in supporting proliferation of mouse Lin−c-Kit+ cells, suggesting that agrin plays a role in the hematopoietic cell development. (ox.ac.uk)
In parallel, mesenchymal stem cells (MSCs) gained attention for their regenerative potential. (mornd.com)
This kind of plasticity has been suggested to be a fundamental feature of mesenchymal stem cells (MSCs) as well. (biomedcentral.com)
Mesenchymal stem cells (MSCs) are multi-potent cells that persist in adult life in some tissue types, such as bone-marrow stroma, fat, skeletal muscle, and synovium without loosing their capacity to proliferate and differentiate [ 9 , 10 ]. (biomedcentral.com)
6,7 Mesenchymal stem cells (MSCs) have also been isolated from other sources, such as bone marrow, 8 the placenta, 9 muscle, 10 or blood. (jcadonline.com)
Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)

Specifically, about the paracrine activities of amniotic fluid stem cells, renal stem cells, embryonic stem cells, mesenchymal stem cell, induced pluripotent stem cells as well as other stem cells. (biomedcentral.com)
However, the isolation and culture of embryonic stem cells (ESCs) in 1981 by Martin Evans and Matthew Kaufman marked a significant turning point in stem cell research. (mornd.com)
Stem cells can be broadly classified into two main categories: embryonic stem cells (ESCs) and adult stem cells (ASCs). (mornd.com)
Embryonic stem cells have the unique ability to exist in an undifferentiated state indefinitely while also being pluripotent. (papersowl.com)
Using these definitions, embryonic stem cells can be classified as totipotent or pluripotent at different developmental stages and locations, whereas adult stem cells can be categorized as pluripotent, multipotent, or unipotent (Figure 1). (papersowl.com)
Marking a vast leap in reproductive therapy, it became possible to isolate these human embryonic stem cells for reproductive purposes, such as in vitro fertilization. (papersowl.com)
Cell cycle regulation by MicroRNAs in embryonic stem cells. (ca.gov)

HSCs are multipotent progenitor cells that can be found in adult bone marrow, peripheral blood, and umbilical cord blood. (encyclopedia.pub)
With growth, these proliferate and, in a remarkably articulated manner, progressively differentiate into multipotent stem cells (Type B), progenitor cells (Type C), mature body somatic cells (Type E), and many eventually become senescent cells (Type E). (anti-agingfirewalls.com)
In essence, early-on the body sets up pools of stem and progenitor cells to replace lost somatic cells. (anti-agingfirewalls.com)
Intradermal adipocyte progenitor cells are activated toward the end of the telogen phase to proliferate and differentiate into mature adipocytes. (jcadonline.com)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)

Figure 2: Manipulating stem cell osteogenesis and proliferation by controlling the elasticity of the bulk phase of void-forming hydrogels. (nature.com)
Mesenchymal stromal cells are essential components of hematopoietic stem and progenitor cell (HSPC) niches, regulating HSPC proliferation and fates. (gene-tools.com)
2 LSCs differ from normal hematopoietic stem cells (HSCs) in that they have activation of key pathways regulating self-renewal, proliferation, and survival. (ashpublications.org)
STIM deficiency did not affect GC B cell proliferation and antigen presentation but led to the enhancement of apoptosis due to the impaired upregulation of anti-apoptotic Bcl2a1. (bvsalud.org)
It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
Contains a viable cell concentrate that optimizes function of the growth factor and progenitor regenerative cell milieu.This promotes better cell proliferation, chemotaxis, migration, and expression, all leading to a profound improvement in clinical outcomes. (realcell.com)
Here we show that agrin, a proteoglycan involved in the neuromuscular junction, is a critical niche-derived signal that controls survival and proliferation of HSCs. (ox.ac.uk)
Subsequently, reactivation permits the proliferation and growth of the tumor cells, giving rise to a macro-metastasis displayed clinically as a bone metastatic lesion. (scite.ai)
Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
In tissue engineering, designs of scaffolds are crucial for cell attachment and proliferation. (mdpi.com)
Embryonic stem cell-specific microRNAs regulate the G1-S transition and promote rapid proliferation. (ca.gov)
Direct invasion by carcinoma cells of the stromal compartment involves active proteolysis effected principally by matrix metalloproteinases (MMPs), while degrading the BM and other ECM that lie in the path of invading tumor cells, MMP-expressing cells also liberate growth factors that are sequestered there, thereby fostering cancer cell proliferation. (medscape.com)

However, recent key findings point to cell-extrinsic factors in the development of leukemia, 3 LSC engraftment into the hematopoietic microenvironment, 4 survival after chemotherapy, 5 and even the determination of leukemia phenotype, 6 suggesting that components of the normal HSC niche, including osteoblastic cells, may have important roles in leukemia pathogenesis. (ashpublications.org)
A pioneer study conducted by Stephen Paget in 1889 hypothesized that metastasis is based on the interplay between the so-called 'seeds' (namely the cancer cells) and the 'soil' (or the host microenvironment) [ 1 ] . (encyclopedia.pub)
Nowadays, this particular microenvironment is usually referred to as 'pre-metastatic niche' [ 5 ] [ 7 ] . (encyclopedia.pub)
The metastatic niche theory suggests that a properly favorable microenvironment (pre-metastatic niche) supports tumor cells to engraft (metastatic niche) and proliferate at secondary sites (micro- to macrometastatic transition). (encyclopedia.pub)
In particular, the pre-metastatic niche results from the synergic interaction between the endogenous organ microenvironment and specific factors secreted by primary tumors [ 7 ] [ 8 ] . (encyclopedia.pub)
The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
The self-renewal and differentiation potential of stem cells is maintained by their interaction with a specialized microenvironment called the stem cell niche. (kyushu-u.ac.jp)
In contrast, cancer cells shift their metabolism toward lactate production even in the presence of oxygen [ 4 ], partly through genetic modifications that stabilize the transcription factor Hypoxia Inducible Factor (HIF) involved in the adaptation of the cells to hypoxia, under nonhypoxic conditions as well as generating an adaptive response to the hypoxic microenvironment (Figure 1 ). (hindawi.com)
These steps are supported by functions of the cancer cells themselves or of the tumor microenvironment. (medscape.com)

Niche-mediated depletion of the normal hematopoietic stem cell reservoir by Flt3-ITD-induced myeloproliferation. (ox.ac.uk)

The hierarchical lineages of HSCs consist of myeloid cells, B lymphocytes, and osteoclasts [ 14 ] . (encyclopedia.pub)
Abstract Hematopoiesis is the process leading to the sustained production of blood cells by hematopoietic stem cells (HSCs). (ox.ac.uk)
Growth, survival, and differentiation of HSCs occur in specialized microenvironments called "hematopoietic niches," through molecular cues that are only partially understood. (ox.ac.uk)
Although previous studies suggested that the expression of FMS-like tyrosine kinase 3 (Flt3) initiates downstream of mouse hematopoietic stem cells (HSCs), FLT3 internal tandem duplications (FLT3 ITDs) have recently been suggested to intrinsically suppress HSCs. (ox.ac.uk)
Herein, single-cell interrogation found Flt3 mRNA expression to be absent in the large majority of phenotypic HSCs, with a strong negative correlation between Flt3 and HSC-associated gene expression. (ox.ac.uk)
Competitive transplantation experiments established that Flt3-ITD compromises HSCs through an extrinsically mediated mechanism of disrupting HSC-supporting bone marrow stromal cells, with reduced numbers of endothelial and mesenchymal stromal cells showing increased inflammation-associated gene expression. (ox.ac.uk)
Tumor necrosis factor (TNF), a cell-extrinsic potent negative regulator of HSCs, was overexpressed in bone marrow niche cells from FLT3-ITD mice, and anti-TNF treatment partially rescued the HSC phenotype. (ox.ac.uk)

The technology provides a viable cell concentrate in a bone marrow niche that optimizes the function of platelets, total nucleated cells, mesenchymal stem cells and hematopoietic stem cells. (realcell.com)
Aim: Tet2 loss may dysregulate the integrity of bone marrow niche, by which affects the malignant progression. (ox.ac.uk)

Sites of loosely packed extracellular matrix (ECM) also offer stem and progenitor cell optimal space to proliferate and differentiate. (europa.eu)
A ) CD34 + cells from human neurogenic heterotopic ossification (NHO) were isolated using immunomagnetic cell separation and induced to differentiate into macrophages for 2 weeks in specific medium. (jci.org)
The above list is in order of increasing cell-type specificity and decreasing cell-type potency to differentiate into other cell types. (anti-agingfirewalls.com)
Starting at conception and throughout life, all cells on this list except the senescent ones will selectively reproduce and possibly differentiate into cells of types further down in the list. (anti-agingfirewalls.com)
At maturity there are relatively very few Type A cells and a mix of Type B, C and D cells, Type B and C cells typically live in protected stem cell niches where they reproduce and, as-needed differentiate to become the normal working body Type D cells. (anti-agingfirewalls.com)
At an advanced age, the pools of Type B and Type C cells become depleted in part because of replicative senescence and the cells remaining in the pools lose their ability to differentiate as necessary to replace Type D cells. (anti-agingfirewalls.com)
Cells in those pools replicate and differentiate throughout life. (anti-agingfirewalls.com)
Stem cells are undifferentiated cells that possess the remarkable ability to self-renew and differentiate into various specialized cell types. (mornd.com)
The discovery of ESCs offered hope for regenerative medicine due to their pluripotent nature, i.e., the ability to differentiate into any cell type in the body. (mornd.com)
Studies highlighted their ability to differentiate into various cell lineages, including osteocytes, chondrocytes, and adipocytes. (mornd.com)
Stem cells are characterized by their unique ability to self-renew and differentiate along multiple cell lineages, contributing to tissue homeostasis. (kyushu-u.ac.jp)
To delve further into the specifics, embryonic and adult stem cells fall into potency categories reflecting their ability to differentiate: totipotent, pluripotent, multipotent, and unipotent. (papersowl.com)
Until recently, it was believed that they were tissue-specific…however, this concept has been challenged… (multipotent cells) can differentiate in vitro and in vivo into various cell types not only from the tissue of origin" (Can/Hematol 2008). (papersowl.com)
Oligopotent stem cells, though not often widely discussed, have the ability to differentiate into a few cells (Kara et al. (papersowl.com)
Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)
Although tumor-associated angiogenesis has traditionally been defined as the sprouting of new vessels from preexisting vessels, it is becoming clear that the blood vessels that support tumor growth can also originate from cells recruited from the bone marrow or can even differentiate from tumor stem cells (vascular mimicry). (medscape.com)

Both cells' lineages are connected to each other in the bone niche and work together to maintain bone homeostasis, sustaining in particular the osteogenesis, osteoclastogenesis, and hematopoiesis processes. (encyclopedia.pub)
They have the multipotentiality to become adipocytes, chondrocytes, myocytes and other cell lineages. (realcell.com)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)

Stem cell behaviour can also be controlled in vitro by manipulating the elasticity of both porous and non-porous materials, yet translation to therapeutic processes in vivo remains elusive. (nature.com)
Here, by developing injectable, void-forming hydrogels that decouple pore formation from elasticity, we show that mesenchymal stem cell (MSC) osteogenesis in vitro , and cell deployment in vitro and in vivo , can be controlled by modifying, respectively, the hydrogel's elastic modulus or its chemistry. (nature.com)
Figure 3: Controlling cell deployment kinetics from void-forming hydrogels in vitro and in vivo . (nature.com)
This remark is certainly a stunning point in favor of a significant Refametinib contribution to growth development control by FSP-specific Testosterone levels cells in vivo , producing those peptides extremely interesting applicants for the advancement of targeted vaccination strategies. (immune-source.com)
Agrin-deficient mice displayed in vivo apoptosis of CD34+CD135− LSK cells and impaired hematopoiesis, both of which were reverted by an agrin-sufficient stroma. (ox.ac.uk)
Subsequently, recipient bone marrow mesenchymal stem cells (BMMSCs) isolated from SHED-EV-administered MRL/lpr mice were examined for the in vitro and in vivo activity of hematopoietic niche formation and immunoregulation. (elsevierpure.com)
The robustness and efficacy of the retrodifferentiation process in generating unprecedented quantities of stem cells belonging to the three germ layers will enable organ and tissue reconstruction ex vivo, using bio-printing and various scaffold materials. (eurekaselect.com)
Probing cell type-specific functions of Gi in vivo identifies GPCR regulators of insulin secretion. (ca.gov)

FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)
Evidence of temporary airway epithelial repopulation and rare clonal formation by BM-derived cells following naphthalene injury in mice. (ca.gov)
Indian hedgehog regulates intestinal stem cell fate through epithelial-mesenchymal interactions during development. (ca.gov)
This is achieved via a process known as epithelial-to-mesenchymal transition (EMT). (medscape.com)
The EMT program dissociates the cells within epithelial cell sheets into individual cells that exhibit multiple mesenchymal attributes. (medscape.com)
[ 4 ] EMT programs are orchestrated by a set of pleiotropically acting transcription factors, including Slug, Snail, Twist, ZEB1, and ZEB2, which organize entrance into a mesenchymal state by suppressing expression of epithelial markers (E-cadherin) and inducing expression of other markers associated with the mesenchymal state. (medscape.com)

Our findings show that biophysical cues can be harnessed to direct therapeutic stem cell behaviours in situ . (nature.com)
Our results provide strong evidence that macrophages contribute to NHO formation through the osteogenic action of OSM on muscle cells within an inflammatory context and suggest that OSM/OSMR could be a suitable therapeutic target. (jci.org)
In order to find more promising treatments to stimulate renal repair, stem cell-based technology has been proposed as a potentially therapeutic option. (biomedcentral.com)
So as a new therapeutic approach, stem cells are under research with a focus on reducing the burden of several kidney diseases. (biomedcentral.com)
The vision of Hong Qian group is to unravel the role of hematopoietic niche including mesenchymal stem cells in myeloid leukemia using patient samples and mouse models, ultimately, to identify new niche- based therapeutic targets for better treatment outcome of diseases. (varbi.com)
Over the years, scientists have unlocked the potential of stem cells, opening up new possibilities in regenerative medicine and providing novel therapeutic approaches for currently incurable diseases. (mornd.com)
Before delving into the molecular biology and therapeutic potentials of induced pluripotent stem cells, it is crucial to provide foundational definitions and descriptions. (papersowl.com)

A particular stem cell type in the adult human-being is the 'Mesenchymal stem cell' (MSC). (europa.eu)
Life depends on constant replenishment of human body cells with new cells created by differentiation of adult stem cells. (anti-agingfirewalls.com)
IF we could find a way to rejuvenate adult stem cells in their niches, then the stem cell supply chain could possibly be transformed from being a once-through-in-life process to a continuing closed-loop process. (anti-agingfirewalls.com)
ESCs are derived from the inner cell mass of blastocysts, whereas ASCs are present in adult tissues and can be found in organs such as bone marrow, adipose tissue, and brain. (mornd.com)
Furthermore, induced pluripotent stem cells (iPSCs) represent a third type, reprogrammed from adult cells by introducing specific transcription factors. (mornd.com)
That is to say, one type of stem cell may be capable of differentiating into all adult cell types, while another may only be capable of maturing into one specific somatic cell type. (papersowl.com)
On a macro scale, the two classifications of stem cells are embryonic and adult stem cells. (papersowl.com)
In contrast, adult stem cells can only give rise to differentiated somatic cells of the particular tissue from which these cells originated (Can/Hematol 2008). (papersowl.com)
In the early 1980s, scientists began to exploit two major classes of stem cells: embryonic and non-embryonic, or adult stem cells. (papersowl.com)
However, the crowning achievement arose in 2006 when researchers at Kyoto University discovered the necessary means to reprogram differentiated adult cells to possess embryonic stem cell-like functionality, as if reversing time itself. (papersowl.com)
The generation and maintenance of replenishing tissues relies on an appropriately regulated balance between self-renewal and differentiation within a relatively small population of adult stem cells. (biomedcentral.com)
Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)

Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
thus, a successful metastatic colony depends on the ability of cancer cells to appropriate distinct microenvironments at each step in the metastatic cascade: the primary tumor, systemic circulation, and the final metastatic destination. (medscape.com)

Stem cells are essential for regeneration and repair of worn-out or injured tissue. (europa.eu)
In zebrafish, we previously found that the stromal cells of the caudal hematopoietic tissue (CHT), a niche functionally homologous to the mammalian fetal liver, arise from the ventral part of caudal somites. (gene-tools.com)
Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
The adipose tissue has progenitor stem cells that consist of cell types capable of self-renewal and differentiation. (realcell.com)
ASCs possess a more limited differentiation potential compared to ESCs, primarily giving rise to cells specific to their tissue of origin. (mornd.com)
Typically, rapidly proliferating tumor cells have glycolytic rates up to 200 times higher than those of their normal tissue of origin, even in the presence of oxygen [ 3 ]. (hindawi.com)
Adipose tissue is a loose connective tissue composed of cells supported by an intracellular matrix as well as by vascular, lymphatic, and neural networks. (jcadonline.com)
During this process, leukocytes lose lineage-associated markers home and undergo homocytic aggregation, upregulate expression of stem cell antigens, and subsequently redifferentiate to give rise original tissue or, transdifferentiate into a different tissue altogether. (eurekaselect.com)
Epimorphic regeneration and retrodifferentiation both have the capacity to recreate and reconstruct tissue with precise positional integration of cells in such a way that will enable us to heal without scars and to understand how to maintain tissue integrity and architecture in the face of a hostile environment. (eurekaselect.com)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)

To ensure survival within the bone marrow, tumor cells must initially colonize a niche in which they can enter dormancy. (scite.ai)

Thus the sclerotome contributes essential stromal cells for each of the key steps of developmental hematopoiesis. (gene-tools.com)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

This special type of stem cell is present in many tissues and restores bone, connective tissues and fat. (europa.eu)
Stem cell technology has garner importance over the last few years as it is used to treat the diseased organs, damaged tissues and regenerative medicines. (precedenceresearch.com)
Stell cells hold great potential for regenerating damaged or diseased tissues and organs. (precedenceresearch.com)
Totipotent stem cells contain all the constituents necessary to produce a living being, given that these cells can supply all embryonic and extraembryonic tissues required for proper growth (Can/Hematol 2008). (papersowl.com)
Biomimicking approaches can be implemented toward reconstitution of the constitutive elements of the human skeletal or hematopoietic tissues. (bourginelab.com)
It has been found that some of the SVF cell types possess regenerative and anti-inflammatory potentials in damaged tissues due to their ability to secrete growth factors and anti-inflammatory molecules. (jcadonline.com)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)

Researchers focused on improving transplantation techniques and understanding stem cell niche interactions to enhance engraftment and minimize complications. (mornd.com)
However, the organisation of stem cell populations is strongly influenced by environmental factors such as specific cell-cell interactions, growth factor and oxygen supply, as well as the geometry and mechanical properties of the local environment [ 3 , 4 ]. (biomedcentral.com)
These models offers a customizable set-up to study complex behaviours/interactions of stem and committed cells, within a fully controlled environment. (bourginelab.com)
Intra-endodermal interactions are required for pancreatic beta cell induction. (ca.gov)
This process is marked by a complex and coordinated set of molecular changes leading to the motile behavior of the invading cancer cells, which involves dynamic cytoskeletal changes, cell-matrix interactions, localized proteolysis, actin-myosin contractions, and focal contact disassembly. (medscape.com)

Adipose cells organized in small clusters under the reticular dermis closely interact with hair follicular cells and regulate the hair cycle. (jcadonline.com)
Safe and Efficacious Use of Secretome From Fibroblasts and Adipose-derived (but not Bone Marrow-derived) Mesenchymal Stem Cells for Skin Therapeutics. (barefacedtruth.com)

The hematopoietic retrodifferentiated stem cells have been shown to engraft an animal host in two proofs of principle clinical studies, demonstrating long-term engraftment and safety in acquired aplastic anaemia, while transient amelioration of beta thalassemia major was also observed. (eurekaselect.com)

Our results now suggest that 4-MU treatment of MSC not only affects the formation of SG via increased levels of O-linked glycosylation of cellular proteins but also that MSC cultivated under osteogenic stimulation develop SG, which in turn support MSC differentiation into osteogenic precursor cells. (europa.eu)

26,27 Dermal adipocytes are organized in small clusters under the reticular dermis and interact with the hair follicular cells regulating the hair cycle. (jcadonline.com)

We demonstrate that oncostatin M (OSM) produced by activated macrophages promotes osteoblastic differentiation and mineralization of human muscle-derived stromal cells surrounding NHOs. (jci.org)
In leukemia (right side of figure), invasion of leukemia cells (purple) results in osteopenia mediated by an expansion of osteoclasts causing increased bone resorption and a concomitant reduction of osteoblastic activity. (ashpublications.org)

Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
Moreover, hematopoietic stem cell transplantation (HSCT) continued to be a successful treatment modality for various blood disorders. (mornd.com)
Systemic transplantation of stem cells from human exfoliated deciduous teeth (SHED) is used to treat systemic lupus erythematosus (SLE)-like disorders in MRL/lpr mice. (elsevierpure.com)

Background: Stromal cells are a functionally important component of human carcinomas. (immune-source.com)
Activation of these pathways is sufficient in many models for full leukemogenesis, suggesting that the LSC niche may be an attractive, but functionally redundant contributor to LSC survival. (ashpublications.org)

We have now found that this ventral domain is the sclerotome, and that two markers of mammalian mesenchymal stem/stromal cells, Alcam and Pdgfr-α, are distinctively expressed there and instrumental for the emergence and migration of stromal cell progenitors, which in turn conditions the proper assembly of the vascular component of the CHT niche. (gene-tools.com)
Current Research and Scholarly Interests The Wang lab takes an interdisciplinary approach to studying fundamental mechanisms controlling gene expression in mammalian cells, and how epigenetic mechanisms such as DNA methylation, chromatin modifications, and RNA influence chromatin dynamics to affect gene regulation. (stanford.edu)

Monitoring the fates of individual cells in high and low oxygen culture, we calculated the average transition times of individual cells into stem cell and differentiated states. (biomedcentral.com)

Our findings demonstrate for the first time that molecular changes in osteoblasts influence the susceptibility to swelling by altering evasion of innate immune cells from your bone Lesopitron dihydrochloride marrow space. (informationalwebs.com)

The effectiveness of stem cell therapies has been hampered by cell death and limited control over fate. (nature.com)
In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
Stem cell products are widely used in various applications that include research and therapies such as medicines. (precedenceresearch.com)
The rising need for effective stem cell therapies for neurological disorders is leading to a surge in research and development activities in the market. (precedenceresearch.com)
Developing stem cell-based therapies is also costly and time-consuming. (precedenceresearch.com)
With a balanced approach, we explore the potential of stem cells as regenerative therapies, analyze the ethical concerns raised by their application, and propose guidelines to ensure responsible and widespread usage. (mornd.com)
They also stress the importance of rigorous research design, peer review, and patient safety in stem cell-based therapies. (mornd.com)
Cell based therapies: Academic or commercial? (ehaweb.org)
Myelin Restoration: Progress and Prospects for Human Cell Replacement Therapies. (ca.gov)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)

Background/aimsMenstrual blood-derived endometrial stem cells (MenSCs) emerge as an ideal source for cell-based treatment in regenerative medicine and immunotherapy. (scite.ai)
Although stem cell research holds tremendous promise in regenerative medicine, ethical concerns have arisen due to the use of ESCs and embryo destruction. (mornd.com)
In conclusion, stem cell research has shown remarkable progress in the year 2000, resulting in significant advancements in the field of regenerative medicine. (mornd.com)
Atta-ur-Rahman, Shazia Anjum , " Frontiers in Stem Cell and Regenerative Medicine Research ", Bentham Science Publishers (2017). (eurekaselect.com)

These problems can be partially circumvented by using macroporous biomaterials that improve the survival of transplanted stem cells and provide molecular cues to direct cell phenotype. (nature.com)
The differentiation of neural stem cells is regulated in a sophisticated manner both temporally and spatially, involving not only cross-talks between extracellular cues but also the intracellular epigenetic programs (DNA methylation, histone modifications, non-coding RNA, etc). (kyushu-u.ac.jp)

These findings suggest that STIM-mediated survival signals after antigen capture regulate the optimal selection and maintenance of GC B cells. (bvsalud.org)
Intrapulmonary delivery of bone marrow-derived mesenchymal stem cells improves survival and attenuates endotoxin-induced acute lung injury in mice. (ca.gov)
The metastatic process involves sequential steps, including invasion through the surrounding extracellular matrix (ECM) and stromal cell layers, intravasation into the lumina of blood vessels, and survival in the circulation. (medscape.com)

This complex process is regulated by the interplay of transcription factors with a chromatin environment, both of which provide the epigenetic information maintaining cell-type specific gene expression patterns. (amegroups.org)

These findings, which establish that Flt3-ITD-driven myeloproliferation results in cell-extrinsic suppression of the normal HSC reservoir, are of relevance for several aspects of acute myeloid leukemia biology. (ox.ac.uk)

Our laboratory is attempting to elucidate mechanisms for fate specification of neural stem cells and to apply the findings from such studies to facilitate the repair and regeneration of injured nerve functions. (kyushu-u.ac.jp)
During limb epimorphic regeneration: committed mesenchymal cells at the stump site dedifferentiate, forming a cluster of heterogeneous population of stem cells, known as the blastema. (eurekaselect.com)

Sialoadhesin is a cell adhesion molecule found on the surface of macrophages. (wikipedia.org)
Sialoadhesin (CD169) positive macrophages, along with mesenchymal stem cells and beta-adrenergic neurons, form the hematopoietic stem cell niche in the bone marrow. (wikipedia.org)
CD169+ macrophages mediate signaling between the various cells and seem to promote hematopoietic stem cell retention to the niche. (wikipedia.org)
B ) Activated macrophages derived in vitro from NHO CD34 + cells stimulate NHO muscle-derived stromal cells (NHO-MDSCs). (jci.org)
C ) Mononuclear cells from human NHO marrows were analyzed by flow cytometry for mac markers, as in A . ( D ) Medium conditioned by activated macrophages from human NHO stimulate NHO-MDSC mineralization. (jci.org)
In that purpose, CD14 + monocytes/macrophages from human NHO were isolated using immunomagnetic cell separation and cultured with or without LPS (100 ng/ml) for 3 days. (jci.org)

The extracellular matrix (ECM) provides structural and biochemical support to the surrounding cells, and it has been used as a coating substrate for cell culture. (hindawi.com)
One of the most basic features is cell invasion and movement through the extracellular matrix. (medscape.com)

According to this concept, MSC plasticity bases on permanent fluctuations of the differentiation state of each individual cell, which enables more differentiated cells to re-gain stem cell properties and subsequently to switch lineage (details see below). (biomedcentral.com)
In response to ligation of monomorphic regions of MHC class II antigens with monoclonal antibody CR3/43, human leukocytes retrodifferentiate into a variety of heterogeneous stem cell types belonging to the mesoderm, ectoderm or endoderm lineage, depending on culture media and conditions. (eurekaselect.com)

The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells. (nature.com)

Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate. (nature.com)
Khetan, S. & Burdick, J. A. Patterning network structure to spatially control cellular remodeling and stem cell fate within 3-dimensional hydrogels. (nature.com)
We are investigating the functional role of microenvironmental "niche" factors and intracellular signaling networks induced by niche signaling in the regulation of cell fate decisions in normal and leukemic stem cells. (kyushu-u.ac.jp)
The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
In normal cells, the fate of pyruvate depends on many factors, one of which is oxygen availability. (hindawi.com)
Dlx1 and Dlx2 control neuronal versus oligodendroglial cell fate acquisition in the developing forebrain. (ca.gov)

The systemic SHED-EV infusion ameliorated the SLE-like phenotypes in MRL/lpr mice and improved the functions of recipient BMMSCs by rescuing Tert mRNA-associated telomerase activity, hematopoietic niche formation, and immunoregulation. (elsevierpure.com)

Analysis of the regulation mechanism of symmetric and asymmetric division of stem cells by machine learning models. (kyushu-u.ac.jp)
Hypoxia leads to the up-regulation of hypoxia-induced transcription factors (HIF)-a and HIF-2a, which are the master regulators of proangiogenic signals, mainly the vascular endothelial cell growth factors (VEGFs). (medscape.com)

2016 . DOI : 10.1016/j.stem.2015.09.019. (epfl.ch)

Cellular senescence refers to active cells that eventually enter a state of irreversible growth arrest. (hindawi.com)
When evaluating with microsatellite steady tumors, there is normally some proof for Kitty least partialC immunological development control in MSI malignancies, like (I) the thick regional lymphocytic infiltration (Crohn's-like lymphocytic response), (II) the elevated apoptotic growth cell amount, and (3) the low amount of isolated metastases that (4) network marketing leads to an improved general individual success [1]C[3]. (immune-source.com)
In the multistep procedure of carcinogenesis, mutations impacting genetics, whose adjustments are beneficial to the growth cell, will be selected positively. (immune-source.com)
The Refametinib ending changed necessary protein typically additionally absence regular efficiency but, they constitute neo-epitopes, when provided in the circumstance of MHC elements at the growth cells' surface area. (immune-source.com)
Growing investment in stem cells research and the exponential success of regenerative medicines are projected to propel the market growth. (precedenceresearch.com)
Another growth rendering factor for the global stem cells market is increasing number of clinical trials across the globe. (precedenceresearch.com)
Increasing number of stem cells banking and government initiatives drives the growth of the stem cells market in emerging countries. (precedenceresearch.com)
Nonetheless, growing stem cell-based research and investment by pharmaceutical and biopharmaceutical companies will significantly propel the market growth over the forecast period. (precedenceresearch.com)
However, some countries and regions have put restrictions on certain stell cell research which is further limiting the growth of the market. (precedenceresearch.com)
The high cost associated with stem cells is observed to limit the growth of the market. (precedenceresearch.com)
Overall, the presence of stem cells is essential for proper human development and function as they contribute to the growth, maintenance, and repair of numerous physiological systems (Kara et al. (papersowl.com)
Cell-to-cell signalling is induced by specific ligands such as growth factors which activate their cognate receptor molecules. (amegroups.org)

Although there are extensive data implicating a pivotal role of TET2 in hematopoietic stem/progenitor cell (HSPCs), the importance of TET2 in bone marrow mesenchymal stromal cells (BMSCs) remains unknown. (ox.ac.uk)

2018 . DOI : 10.1016/j.cell.2018.01.028. (epfl.ch)
Cell Stem Cell 2018. (bourginelab.com)

Ever since descriptions of the various functional components of the hematopoietic stem cell niche, leukemia researchers have wondered whether leukemia stem cells (LSCs) require similar cell-extrinsic support for long-term maintenance of leukemia. (ashpublications.org)
Identifying niche cells in hematopoietic and leukemia stem cells using single-cell analysis and 3D bone marrow imaging. (kyushu-u.ac.jp)

The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)

Sialoadhesin predominantly binds neutrophils, but can also bind monocytes, natural killer cells, B cells and a subset of cytotoxic T cells by interacting with sialic acid molecules in the ligands on their surfaces. (wikipedia.org)

The successful candidate will conduct the research with a special focus on deciphering molecular pathways mediating leukemic stem cell interaction with their niche under the supervision of the group leader (Dr. Hong Qian). (varbi.com)

We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)
Inflammation is a process driven by underlying cell-cell communication and many other factors. (mdpi.com)
Gene expression patterns of human colon tops and basal crypts and BMP antagonists as intestinal stem cell niche factors. (ca.gov)

In vitro cultivated stem cell populations are in general heterogeneous with respect to their expression of differentiation markers. (biomedcentral.com)
In hematopoietic progenitor populations, this heterogeneity has been shown to regenerate within days from isolated subpopulations defined by high or low marker expression. (biomedcentral.com)
Simulation results on the composition of the functional stem cell pool of MSC populations suggest that most of the cells that constitute this pool originate from more differentiated cells. (biomedcentral.com)

they continue producing neurons and glial cells every day, suggesting that the new neurons produced by them are implicated in higher-order function of the brain such as learning and memory. (kyushu-u.ac.jp)

Anatomy60

Organisms14

Diseases9

Chemicals and Drugs45

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Phenomena and Processes46

Disciplines and Occupations3

Technology, Industry, Agriculture4

Named Groups1

Health Care1

MSCs21

Embryonic stem7

Progenitor cells5

Proliferation14

Microenvironment9

Normal hematopoietic1

HSCs7

Bone marrow niche2

Differentiate16

Lineages3

Vivo8

Epithelial6

Therapeutic7

Adult12

Microenvironments2

Tissue10

Tumor cells1

Hematopoiesis2

Tissues8

Interactions5

Adipose2

Engraftment1

Osteogenic1

Adipocytes1

Osteoblastic2

Transplantation3

Functionally2

Mammalian2

Transition1

Osteoblasts1

Therapies10

Regenerative medicine4

Cues2

Survival3

Chromatin1

Myeloid leukemia1

Regeneration2

Macrophages6

Extracellular matrix2

Lineage2

Osteogenesis1

Fate6

Mice1

Regulation2

20161

Growth12

BMSCs1

20182

Leukemia stem cells2

Fates1

Monocytes1

Leukemic stem1

Factors3

Populations3

Neurons1