• Bone marrow mesenchymal stem cells (MSCs) have been identified to have the potential to differentiate into multiple types of cells. (nih.gov)
  • However, the rejection reaction often occurs in the transplantation of MSCs and could induce cell death. (nih.gov)
  • MSCs were isolated from bone marrow in mice femoral bone marrow. (nih.gov)
  • The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
  • Mesenchymal stem cells (MSCs) support chondrogenic differentiation and are an attractive cell source for cartilage tissue engineering. (nature.com)
  • Mesenchymal stem cells (MSCs) can differentiate into chondrocytes, while mechanical loading has been proposed as alternative strategy to induce chondrogenesis excluding the use of exogenous factors. (nature.com)
  • Mesenchymal stem cells (MSCs) are multipotent stem cells with a high capacity for self-renewal. (mbpinc.net)
  • Fibronectin (FN) is commonly used in the development of serum-free media for the expansion of mesenchymal stem cells (MSCs). (biomedcentral.com)
  • This study was aimed to observe if thrombin could stimulate FN secretion by human bone marrow MSCs and investigate the potential underlying mechanisms. (biomedcentral.com)
  • PCR analysis showed that human bone marrow MSCs expressed two subtypes of PARs, PAR-1 and PAR-2. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) are adult multipotent cells that were initially isolated from bone marrow[ 1 ] and characterized by the fibroblast-like appearance in culture and the capacities to form bone, adipose and cartilage. (biomedcentral.com)
  • However, the quantity of MSCs is very low in bone marrow (about 0.001 to 0.01% of the mononuclear cells) and in vitro expansion is the prerequisite for their clinical application. (biomedcentral.com)
  • We analyzed expanded mesenchymal stem cells (MSCs) isolated from sporadic ALS patients and compared them with MSCs isolated from healthy donors. (unito.it)
  • MSCs were isolated from bone marrow by Percoll gradient and maintained in culture in MSC Medium until the third passage. (unito.it)
  • These results suggest that extensive in vitro expansion of patient MSCs does not involve any functional modification of the cells, including chromosomal alterations or cellular senescence. (unito.it)
  • Hence, there is a good chance that MSCs might be used as a cell-based therapy for ALS patients. (unito.it)
  • In addition, bone marrow (BM)-derived mesenchymal stem cells (MSCs) have been suggested as an effective therapy for hepatic fibrosis and cirrhosis. (elsevierpure.com)
  • The aim of this study was to determine whether the combination treatment of simvastatin plus MSCs (Sim-MSCs) could have a synergistic effect on hepatic fibrosis in a thioacetamide (TAA)-induced cirrhotic rat model and hepatic stellate cells (HSCs). (elsevierpure.com)
  • In addition to providing microenvironmental support for hematopoietic processes, BM-MSCs can differentiate into various mesodermal lineages including osteoblast/osteocyte, chondrocyte, and adipocyte that are crucial for bone metabolism. (ijbs.com)
  • While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. (ijbs.com)
  • To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271 + BM-derived mononuclear cells (BM-MNCs) from two human subjects. (ijbs.com)
  • We successfully identified LEPR hi CD45 low BM-MSCs within the CD271 + BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. (ijbs.com)
  • We describe a novel therapeutic approach for cirrhosis using mesenchymal stem cells (MSCs) and colony‐stimulating factor‐1‐induced bone marrow‐derived macrophages (id‐BMMs) and analyze the mechanisms underlying fibrosis improvement and regeneration. (birmingham.ac.uk)
  • Mouse MSCs and id‐BMMs were cultured from mouse bone marrow and their interactions analyzed in vitro. (birmingham.ac.uk)
  • Intravital imaging showed that after combination cell administration, a large number of id‐BMMs, which phagocytosed hepatocyte debris and were retained in the liver for more than 7 days, along with a few MSCs, the majority of which were trapped in the lung, migrated to the fibrotic area in the liver. (birmingham.ac.uk)
  • Indirect effector MSCs and direct effector id‐BMMs synergistically improved cirrhosis along with host cells in mice. (birmingham.ac.uk)
  • The key attributes like isolation efficiency, cell yield, colony-forming unit-fibroblast (CFU-F) frequency, phenotypic characteristics, and multi-lineage differentiation capacity were determined for the isolated MSCs from bone marrow specimens. (journalcra.com)
  • Further, when those MSCs were analyzed for their specific marker expression using flowcytometry, they were found to be positive for CD29, CD73, CD90, and CD105 and negative for CD34 and CD45 stem cell markers respectively. (journalcra.com)
  • Apart from this, it was observed that those MSCs, when induced to differentiate into cardiomyocytes, they successfully differentiated into cardiomyocyte like cells with flat, wide, myotube like structure with sarcomeric organization, which were later confirmed to be cardiomyocytes by qRT-PCR. (journalcra.com)
  • Thus, this study shows that MSCs were isolated from human bone-marrow and subsequently differentiated into cardiomyocytes with 100% success rate. (journalcra.com)
  • Studies on therapeutic administration of bone marrow-derived mesenchymal stem cells (MSCs) in ALI caused by the viral infection have been limited in number and have shown conflicting results. (unair.ac.id)
  • Methods: MSCs were prepared from the bone marrow of 9 to 12 week-old BALB/c mice. (unair.ac.id)
  • Conclusion: The administration of MSCs prevented further lung injury and inflammation, and enhanced alveolar cell type II and I regeneration, while it did not significantly affect viral proliferation and mouse morbidity and mortality. (unair.ac.id)
  • Recent findings suggest that bone marrow-derived mesenchymal stem cells (BM-MSCs) are recruited into the microenvironment of developing tumors, where they contribute to metastatic processes. (houstonmethodist.org)
  • The aim of this study was to investigate the role of BM-MSCs in promoting osteosarcoma and hepatocellular carcinoma cell progression in vitro and the possible mechanisms involved in these processes. (houstonmethodist.org)
  • U2OS and SNU-398 are osteosarcoma and hepatocellular carcinoma cell lines, respectively, that can be induced to proliferate when cultured in the presence of BM-MSCs. (houstonmethodist.org)
  • To determine the effect of BM-MSCs on U2OS and SNU-398 cells, the AKT and ERK signaling pathways were investigated, and increases were observed in active P-Akt and P-Erk forms. (houstonmethodist.org)
  • Moreover, BM-MSCs caused an increase in tumor cell migration and invasion that was derived from the enhancement of CXCR4 levels.Thus, when tumor cells were treated with the CXCR4 antagonist AMD3100, a reduction in their migration and invasion was observed. (houstonmethodist.org)
  • Taken together, these results suggest that inhibiting CXCR4 impairs the cross-talk between tumor cells and BM-MSCs, resulting in reduced metastatic potential in osteosarcoma and hepatocellular carcinoma cells. (houstonmethodist.org)
  • AIM: Bone marrow-derived mesenchymal stem cells (BM-MSCs) are the most well-studied and characterised stem cell types. (wustl.edu)
  • Introduction: Mesenchymal stem cells (MSCs) can be isolated from adult bone marrow (BM), expanded, and differentiated into several cell types, including chondrocytes. (elsevierpure.com)
  • Administration of bone tissue marrow-derived mesenchymal stem cells (MSCs) can be an innovative strategy for the treating a variety of diseases that aren't curable by current therapies including center failure. (bio-biz-navi.com)
  • This review content summarizes protocols to isolate and broaden bone tissue marrow-derived MSCs in 47 latest scientific studies of MSC-based therapy that have been released after 2007 onwards and supplied sufficient methodological details. (bio-biz-navi.com)
  • 1 Launch Recent research provides extensively proven the potential of bone tissue marrow- (BM-) produced mesenchymal stem cells (MSCs) for regenerative remedies in a variety of organs like the center [1]. (bio-biz-navi.com)
  • Because the initial scientific trial of BMC shot in 1995 [3] a lot more than 2 0 sufferers have been implemented with allogeneic or autologous MSCs for the treating various illnesses including graft-versus-host disease hematologic malignancies cardiovascular illnesses neurologic illnesses autoimmune diseases body organ transplantation refractory wounds and bone tissue/cartilage flaws [4]. (bio-biz-navi.com)
  • MSCs stand for 0.001-0.01% of BM mononuclear cells or lower [8]. (bio-biz-navi.com)
  • Here, by using a microarray analysis, we demonstrate that human BM mesenchymal stromal/stem cells (MSCs) in an early osteoinductive stage (e-MSCs) are characterized by unique hematopoiesis-associated gene expression with an enhanced hematopoiesis-supportive ability. (elsevierpure.com)
  • In comparison to BM-MSCs without osteoinductive treatment, gene expression in e-MSCs was significantly altered in terms of their cell adhesion- and chemotaxis-related profiles, as identified with Gene Ontology and Gene Set Enrichment Analysis. (elsevierpure.com)
  • Noteworthy, expression of the hematopoiesis-associated molecules CXCL12 and vascular cell adhesion molecule 1 was remarkably decreased in e-MSCs. (elsevierpure.com)
  • e-MSCs supported an enhanced expansion of CD34 + hematopoietic stem and progenitor cells, and generation of myeloid lineage cells in vitro. (elsevierpure.com)
  • e-MSCs exhibited the absence of decreased stemness-associated gene expression, increased osteogenesis-associated gene expression, and apparent mineralization, thus maintaining the ability to differentiate into adipogenic cells. (elsevierpure.com)
  • MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
  • The OriCell Mouse Bone Marrow Mesenchymal Stem Cells Complete Medium, carefully developed by the OriCellTM R&D team, contains a basic medium suitable for the growth of Mouse Bone Marrow Mesenchymal Stem Cells, OriCell Premium Fetal Bovine Serum and other additives required for cell growth. (gentaur.be)
  • A large number of cell culture data verify that this product can maintain a good growth state of Mouse Bone Marrow Mesenchymal Stem Cells in vitro for a long time. (gentaur.be)
  • Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers. (lu.se)
  • Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers significant implications for the study of cartilage biology and the discovery of potential therapeutic interventions for cartilage-related and associated disorders. (lu.se)
  • Bone marrow mesenchymal stem cells (BMSCs) have been reported to attenuate myocardial I/R injury via their paracrine effects, which can be enhanced by hypoxic preconditioning. (researchsquare.com)
  • The administration of bone marrow-derived mesenchymal stem cells (BMSCs) is emerging as a promising approach for I/R injury-induced myocardial infarction due to its multiple differentiation potential(7, 8). (researchsquare.com)
  • Bone marrow mesenchymal stem cells (BMSCs) have potential therapeutic benefits for the treatment of endometrial diseases and injury. (biologists.com)
  • BMSCs interact with uterus parenchymal cells by direct contact or indirect secretion of growth factors to promote functional recovery. (biologists.com)
  • Accordingly, investigations on cellular therapies have therefore moved to progenitor cell populations such as bone marrow derived mesenchymal stem cells (BMSCs), which have the ability to differentiate into cartilage cells 4 . (nature.com)
  • Background: Bone marrow mesenchymal stem cells (BMSCs) are easy to isolate and amplify in vitro, and easy for transferring and expressing of exogenous gene. (ox.ac.uk)
  • BMSCs are ideal therapeutic cells and target cells for gene therapy. (ox.ac.uk)
  • Bone marrow mesenchymal stem cells (BMSCs)-derived extracellular vesicles have been applied in many diseases . (bvsalud.org)
  • To determine whether SATB2 overexpression in BMSCs can enhance the osseointegration of implants, BMSCs were infected with the retrovirus encoding Satb2 (pBABE-Satb2) and were locally applied to bone defects before implanting the titanium implants in the mouse femur. (nih.gov)
  • IHC staining analysis revealed that SATB2-overexpressing BMSCs were intensely distributed in the bone tissue surrounding the implant. (nih.gov)
  • Histological analysis showed that SATB2-overexpressing BMSCs significantly enhanced new bone formation and bone-to-implant contact 3 weeks after implantation. (nih.gov)
  • Real-time qRT-PCR results showed that the local delivery of SATB2-overexpressing BMSCs enhanced expression levels of potent osteogenic transcription factors and bone matrix proteins in the implantation sites. (nih.gov)
  • μCT analysis demonstrated that SATB2-overexpressing BMSCs significantly increased the density of the newly formed bone surrounding the implant 3 weeks post-operatively. (nih.gov)
  • Donor variation among patients, cell heterogeneity, and unpredictable capacity for differentiation reduce effectiveness of BMSCs for regenerative cell therapies. (elsevierpure.com)
  • The cell surface glycoprotein CD24 exhibits the most prominent differential expression during osteogenic versus adipogenic differentiation of human BMSCs. (elsevierpure.com)
  • In undifferentiated human BMSCs, CD24 cell surface expression is variable among donors (range: 2%-10%) and increased by two to fourfold upon osteogenic differentiation. (elsevierpure.com)
  • RNAseq analysis of CD24pos and CD24neg BMSCs identified a limited number of genes with increased expression in CD24pos cells that are associated with cell adhesion, motility, and extracellular matrix. (elsevierpure.com)
  • Hence, expression of the cell surface glycoprotein CD24 identifies a subpopulation of human BMSCs with reduced capacity for proliferation and extracellular matrix mineralization. (elsevierpure.com)
  • Functional specialization among BMSCs populations may support their regenerative potential and therapeutic success by accommodating cell activities that promote skeletal tissue formation, homeostasis, and repair. (elsevierpure.com)
  • Bone marrow-derived mesenchymal stem cells (BMSCs) have been reported to have a potential therapeutic role on SAP, but the specific mechanism is unclear. (drcalapai.com)
  • The capacities of proliferation, migration, and repair of human umbilical vein endothelial cells were enhanced by BMSCs supernatant. (drcalapai.com)
  • Background and Purpose: Bone marrow-derived mesenchymal stem cells (BMSCs) are potential in promoting bone regeneration for their multipotential differentiation capacity. (authorea.com)
  • Overexpression of Wnt3a increased CORM-3-induced osteogenic differentiation of rat BMSCs, the opposite effect was observed in Wnt3a-deficient cells. (authorea.com)
  • Conclusion and Implications: These results demonstrated that miR-195-5p may negatively regulate CORM-3-induced osteogenic differentiation of rat BMSCs by targeting Wnt3a, which provided insight into new mechanism of CORM-3, and theoretical basis for bone regeneration. (authorea.com)
  • Bone marrow and adipose tissue have emerged as excellent sources of progenitor cells. (ivis.org)
  • One great advantage of MSC is that these cells may be directly obtained from individual patients, thereby eliminating the complications associated with immune rejection of allogenic tissue and infectious diseases. (biomedcentral.com)
  • Their easy accessibility as well as exceptional genomic stability and their capacity for tissue repair with few immunological and ethical issues make them the favorable cell type for experimental and clinical use. (mbpinc.net)
  • Design, time and setting: The single sample cell gene engineering experiment was performed at the Dalian Research & Development Center for Stem Cells and Tissue Engineering, Dalian University of Technology from March 2006 to April 2007. (ox.ac.uk)
  • Adult mesenchymal stem cells are being used by researchers in the fields of regenerative medicine and tissue engineering to artificially reconstruct human tissue which has been previously damaged. (wikipedia.org)
  • Also, transplanted mesenchymal stem cells pose little risk for rejection as they are derived from the patients own tissue, so are genetically identical, however graft versus host disease is a possibility, where the cells change enough while outside the patient's body that the immune system recognizes them as foreign and can attempt to reject them. (wikipedia.org)
  • Genetic mutations in stem cells as well as conditions at target tissue may result in formation of a cancerous tumor. (wikipedia.org)
  • Umbilical cord blood (UCB) and bone marrow (BM)-derived stem and progenitor cells possess two characteristics required for successful tissue regeneration: extensive proliferative capacity and the ability to differentiate into multiple cell lineages. (ox.ac.uk)
  • Mesenchyme cells, which are derived from bone marrow stem cells, have multiple applications in tissue engineering and regenerative medicine. (ac.ir)
  • The TGFβ-1 protein is found throughout the body but is particularly abundant in tissues that make up the skeleton, where it helps regulate the formation and growth of bone and cartilage, a tough, flexible tissue that makes up much of the skeleton during early development. (medlineplus.gov)
  • Tissue- engineered fabrication of an osteochondral composite graft using rat bone marrow-derived mesenchymal stem cells. (bvsalud.org)
  • In individuals with this condition, pancreatic acinar cells do not develop in utero and are replaced by fatty tissue. (medscape.com)
  • Bone marrow is the soft, spongy, gelatinous tissue found in the hollow spaces in the interior of bones. (medscape.com)
  • Bone marrow consists of stem cells, which are large, "primitive," undifferentiated cells supported by fibrous tissue called stroma. (medscape.com)
  • There are 2 main types of stem cells and, therefore, the bone marrow consists of 2 types of cellular tissue. (medscape.com)
  • Bone marrow can be 1 of 2 types, red or yellow, depending on whether it consists of mainly hematopoietic (and therefore, red-colored) tissue or fatty (and therefore a yellow-colored) tissue. (medscape.com)
  • However, there have been some cases where there were both improvement and toxicity inflicted on the targeted organ, as well as cases where treatment of mesenchymal stem cells did not show improvement of function at all. (wikipedia.org)
  • Several different forms of stem cells have been identified and studied in the field of regenerative medicine. (wikipedia.org)
  • In an interview with Medscape Medical News, Sarin said that although the use of G-CSF for patients with severe SAH is still under investigation at his center, "we are using G-CSF routinely for decompensated cirrhosis, where it is like an in vivo extension of regenerative stem cells. (medscape.com)
  • Each year, the Lund Stem Cell Center recognizes notable scientific achievements made by our members in the fields of stem cell biology and regenerative medicine through our Article of the Year Award. (lu.se)
  • This recognition highlights the outstanding contributions made by our members in advancing the fields of stem cell biology and regenerative medicine. (lu.se)
  • Additionally, it will explain the techniques of collecting, packaging, and transporting bone marrow aspirates for collection, expansion, and 150 injections into diseased tissues. (ivis.org)
  • Hypermethylation-mediated downregulation of long non-coding RNA MEG3 inhibits osteogenic differentiation of bone marrow mesenchymal stem cells and promotes pediatric aplastic anemia. (stembook.org)
  • With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
  • In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
  • Cell replacement therapy has been suggested as a promising therapeutic approach for multiple neurodegenerative diseases, including motor neuron disease. (unito.it)
  • Recent evidence suggests that pharmacological treatment devoted to regulating stem cell function is a potential new therapeutic strategy that is drawing nearer to clinical practice. (elsevierpure.com)
  • In the research process of expanding the therapeutic uses of mesenchymal stem cells, they are grown in laboratories or grown using medication to stimulate new cell growth within the human body. (wikipedia.org)
  • One of the most extensively studied stem cell types are embryonic stem cells, which possess many of the same therapeutic properties as mesenchymal stem cells, including the ability to self-regenerate and differentiate into a number of cell lineages. (wikipedia.org)
  • Also, the host's immune system may reject injected embryonic stem cells and thus eliminate their therapeutic effects. (wikipedia.org)
  • Background: Chondrocytes are the primary cells responsible for maintaining cartilage integrity and function. (lu.se)
  • But the cartilage regeneration capacity of these cells remains unpredictable because of cell heterogeneity. (whiterose.ac.uk)
  • Hypothesis: The harvest technique of BM may highly influence stem cell heterogeneity and, thus, cartilage formation because these cells have distinct spatial localization within BM from the same bone. (whiterose.ac.uk)
  • Currently, the only FDA-approved cell-based therapy for cartilage defects involves autologous chondrocyte implantation: chondrocytes harvested from low-contact areas are expanded in vitro and then re-injected directly into the damaged site 1 . (nature.com)
  • Besides the cell source choice, also mechanical stimuli are crucial in the development and maintenance of articular cartilage. (nature.com)
  • These studies have demonstrated that mechanical forces are able to shape the mesenchymal stem cell (MSC) fate when appropriately applied in in vitro models of cartilage regeneration. (nature.com)
  • Osteoarthritis is a chronic arthropathy characterized by disruption and potential loss of joint cartilage along with other joint changes, including bone hypertrophy (osteophyte formation). (msdmanuals.com)
  • Obesity triggers some of these defects in cartilage metabolism, leading to cartilage matrix damage and subchondral bone remodel-ing mediated by adipokines, such as leptin and adipsin, and compounded by mechanical factors due to excess weight. (msdmanuals.com)
  • They can develop into specialized cells of the body such as chondrocytes/osteoblasts/adipocytes. (mbpinc.net)
  • Chondrocytes have the longest cell cycle in the body (similar to central nervous system and muscle cells). (msdmanuals.com)
  • This may result in clinicians opting out of pursuing this choice of stem cell therapy. (ivis.org)
  • Host macrophages and neutrophils infiltrated after combination therapy and contributed to liver fibrosis regression and promoted regeneration along with administered cells. (birmingham.ac.uk)
  • In mesenchymal stem cell therapy, most of the cells are extracted from the adult patient's bone marrow Mesenchymal stem cells can be obtained via a procedure called bone marrow aspiration. (wikipedia.org)
  • Human mesenchymal stem cell therapy is limited due to variation in individual response to treatment and the high number of cells needed for treatment. (wikipedia.org)
  • Low-level laser therapy has been investigated as a possible stimulus for enhancement of proliferation and differentiation of various cell types, but few reports relate undifferentiated mouse pulp cells (OD-21) response to irradiation in in vitro models. (bvsalud.org)
  • The aim of this study was to analyze the influence of low-level laser therapy (=660 nm), with three different irradiation times, on the behavior of OD-21 cell line. (bvsalud.org)
  • Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
  • Mesenchymal stem cells are able to differentiate, or mature from a less specialized cell to a more specialized cell type, to replace damaged tissues in various organs. (wikipedia.org)
  • Over a course of two or three weeks, the cells will multiply and differentiate into specialized cells. (wikipedia.org)
  • During long-term culture, mesenchymal stem cells age, lose their ability to differentiate, and have a higher chance to undergo malignant transformation. (wikipedia.org)
  • They can differentiate into many cell types including bone, fat, and muscle which allow them to treat a large range of disorders. (wikipedia.org)
  • As needed, the stem cells differentiate to become a particular kind of cell-a white blood cell, red blood cell, or platelet. (medscape.com)
  • T lymphocytes originate via lymphoid stem cells that migrate to the thymus and differentiate under the influence of the thymic hormones thymopoietin and thymosin. (medscape.com)
  • These bone marrow-derived mesenchymal stem cells were isolated from a young adult male Fischer rat leg bone. (mbpinc.net)
  • Adult human bone marrow (BM) is the major source of mesenchymal stem cells (MSC) for cell-based therapies. (journalcra.com)
  • These results, therefore, provide a global view of the signaling and regulatory network that controls oxygen sensing in human adult stem/progenitor cells derived from hematopoietic tissues. (ox.ac.uk)
  • The aim of this study was to analyze the effects of marijuana on differentiation power of bone marrow stem cells of adult male rats in adipose tissues. (ac.ir)
  • In this experimental study, the bone marrow stem cells of adult male rats were segregated with flushing method. (ac.ir)
  • In an adult, roughly half of the bone marrow is still red. (medscape.com)
  • These protective effects may be associated with the inhibition of apoptosis and inflammation, as reflected by less TUNEL-positive cells and lower levels of serum proinflammatory cytokines (Interleukin-1β, Interleukin-6, tumor necrosis factor-α) and cardiac troponin I in the N-CM group compared with the vehicle group. (researchsquare.com)
  • There is a signal crosstalk between autophagy and apoptosis, which may be regulated to produce synergistic effect on the preconditioning of stem cells. (biomedcentral.com)
  • Cell apoptosis assay CAL 27 or HN4 cells were cultured in charge or MSC\CM media at 37C for 48?hours and apoptotic cells were detected using FITC\Annexin V LFNG antibody and propidium iodide (BD Biosciences). (biotech2012.org)
  • Results and Conclusions: IGF-I modulated MSC chondrogenesis by stimulating proliferation, regulating cell apoptosis, and inducing expression of chondrocyte markers. (elsevierpure.com)
  • The TGFβ-1 protein triggers chemical signals that regulate various cell activities, including the growth and division (proliferation) of cells, the maturation of cells to carry out specific functions (differentiation), cell movement (motility), and controlled cell death (apoptosis ). (medlineplus.gov)
  • Hydrogen peroxide was used to induce an oxidative stress model of hepatocyte IAR-20 cells to evaluate the protective effects of BMMSCs in vitro. (medscimonit.com)
  • Fluorosilicic acid induces DNA damage and oxidative stress in bone marrow mesenchymal stem cells. (fluoridealert.org)
  • For analyzing the viability and differentiation power of the cells in adipose tissues in terms of marijuana usage, cell samples were exposed to different dosages of marijuana, such as 30 and 6000 ng/ml and cell survival was evaluated by MTT.The adipogenic differentiation potential of the treated cells were analyzed based on oil red staining. (ac.ir)
  • However, their low frequency in bone marrow necessitate ex vivo expansion for further clinical application. (biomedcentral.com)
  • In previous studies which observed the safety of clinical mesenchymal stem cell use, no serious side effects were noted. (wikipedia.org)
  • These results provide support for future pharmacological and clinical applications of SATB2, which accelerates bone regeneration around titanium implants. (nih.gov)
  • 3. Have a clinical history of malignancy within 2.5 years (i.e., study participants with prior malignancy must be cancer free for 2.5 years) except curatively treated basal cell carcinoma, melanoma in situ, or cervical carcinoma. (who.int)
  • The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
  • Each lot of UBP-0050 is tested to ensure the cells can be passaged at least three times (i.e., approximately 9 to 10 population doublings) after thaw in complete growth media (Mesenchymal Stem Cell Growth Medium (MSCGM), UBP-35), when cultured following the detailed protocol described below). (ubpone.com)
  • growth, invasion, metastasis and enhanced the manifestation of EMT and POSTN in tumor cells. (biotech2012.org)
  • Cell growth and viability were assessed after 7 and 10 days. (bvsalud.org)
  • Methods: CTPs obtained from the femur of patients undergoing total hip replacement by 2 harvest techniques-BM aspiration and BM collection-after bone rasping were immunophenotyped by flow cytometry and evaluated for chondrogenic ability. (whiterose.ac.uk)
  • Mesenchymal stem cells can be easily extracted through well-established procedures such as bone marrow aspiration. (wikipedia.org)
  • Results: Cells from the BM after rasping were significantly more chondrogenic than the donor-matched aspirate, whereas no notable difference in their osteogenic or adipogenic potential was observed. (whiterose.ac.uk)
  • We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
  • In this study, the transcriptional profiles and proliferation and differentiation potential of UCB CD133(+) cells and BM mesenchymal cells (BMMC) exposed to normoxia and hypoxia were analyzed and compared. (ox.ac.uk)
  • The differentiation potential of UCB CD133(+) clonogenic myeloid cells was unaltered by short exposures to hypoxia. (ox.ac.uk)
  • We have isolated a cardiomyogenic cell line (CMG cell) from murine bone marrow mesenchymal stem cells. (elsevierpure.com)
  • Immortalized murine pulp cells (OD-21)6 are considered as the major action mechanism of those lasers.11 undifferentiated mesenchymal stem cells derived from fetal Although this mechanism has not been fully clarified, the most molar papillae7. (bvsalud.org)
  • Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia. (physiciansweekly.com)
  • The exosomes (Exo) from human bone marrow mesenchymal stem cells (hBMSCs) after the transfection of miR mimics/inhibitor/si-PTEN were isolated and used to stimulate M4E and Hep2 cells. (hindawi.com)
  • In this study, exosomes from bone marrow plasma were successfully extracted and identified. (techscience.com)
  • MiR-29a-3p was enriched in hBMSC-derived Exo, and the Exo from miR-29a-3p mimics transfected hBMSCs could inhibit laryngocarcinoma cell malignant phenotypes in vitro and prevent tumor progression in vivo . (hindawi.com)
  • Here we document the effects of specified PEMF parameters over mesenchymal stem cells (MSC) chondrogenic differentiation. (nature.com)
  • To improve the quality of liver grafts from extended-criteria donors donated after circulatory death (DCD), this study explored whether bone marrow mesenchymal stem cells (BMMSCs) combined with normothermic machine perfusion (NMP) have protective effects on DCD donor livers and the effects of ferroptosis in this procedure. (medscimonit.com)
  • Since the iliac crest remains a frequent site of BM harvest for musculoskeletal regeneration, the authors also compared the spatial distribution of these subsets in trabeculae of femoral head and iliac crest and found CD2711CD561 bone-lining cells in both tissues. (whiterose.ac.uk)
  • Fibrosis regression, liver regeneration, and liver‐migrating host cells were evaluated. (birmingham.ac.uk)
  • Bladder regeneration studies have yielded inconclusive results possibly due to the use of unfavorable cells and primitive scaffold design. (northwestern.edu)
  • We hypothesized that human mesenchymal stem cells seeded onto poly(1,8-octanediol-co-citrate) elastomeric thin films would provide a suitable milieu for partial bladder regeneration. (northwestern.edu)
  • The Laboratory of Bone Organ Modeling and Regeneration received the 2023 Article of the Year Award for their research generating human mini-bones in a laboratory setting which mimics the properties of real human bone. (lu.se)
  • Previous studies indicated that exosomal miR-29a-3p derived from human bone mesenchymal stem cells (hBMSCs) could efficiently suppress cell migrative ability and vasculogenic mimicry in glioma [ 15 ]. (hindawi.com)
  • Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
  • In this study we asked if human MSC are developing in an aberrant or unwanted way during ex vivo long-term cultivation and if cultivation conditions exert any influence on their stem cell maintenance. (biomedcentral.com)
  • GENERAL INFORMATION: GFP HBMMSCs (UBP-0050) are isolated from human Bone Marrow tissues and demonstrated with spindle-shaped-, and fibroblast-like cells. (ubpone.com)
  • In the present study, six samples of human bone-marrow were collected. (journalcra.com)
  • And hence, this study suggests that human bone marrow is a potential source of mesenchymal stem cells that could be differentiated in to ccardiomyocytes and used for cell based therapies for treating various cardiovascular diseases. (journalcra.com)
  • Transcriptional profiling of human cord blood CD133+ and cultured bone marrow mesenchymal stem cells in response to hypoxia. (ox.ac.uk)
  • PyV-induced tumorigenesis in laboratory animals, by simian virus 40 (SV40) or by multiple human PyVs, such as JC virus (JCV), is used extensively as a cell transformation model. (cdc.gov)
  • This innovative study by first-author Dr. Ani Grigoryan involved engineering mesenchymal stem cells and guiding them through a process mimicking how bones develop naturally in the human body. (lu.se)
  • A Longitudinal Open-Label Multi-center Single-Arm Phase I/IIa Study to Evaluate the Safety and Preventive Effect on Cardiovascular Events of Human Allogenic Bone-Marrow-Derived Mesenchymal Stromal Cell Product, StromaForte, in Study Participants. (who.int)
  • Expression profile of microRNAs following bone marrow‑derived mesenchymal stem cell treatment in lipopolysaccharide‑induced acute lung injury. (logosbio.com.cn)
  • Shwachman-Diamond syndrome (SDS) is a rare autosomal recessive disorder characterized by exocrine pancreatic insufficiency, bone marrow dysfunction, and skeletal abnormalities. (medscape.com)
  • It is essential that the cells be subculture BEFORE reaching confluence as post-confluent cells exhibit changes in morphology, slower proliferation, and reduced differentiation after passaging. (ubpone.com)
  • The cells showed a fibroblast-like morphology, but the morphology changed after 5-azacytidine exposure. (elsevierpure.com)
  • Within the normal BM and in pathological conditions, areas of hypoxia may have a role in maintaining stem cell fate or determining the fine equilibrium between their proliferation and differentiation. (ox.ac.uk)
  • Shwachman-Diamond syndrome is the second most common cause of inherited pancreatic insufficiency after cystic fibrosis and the third most common inherited bone marrow failure syndrome after Fanconi anemia and Blackfan-Diamond anemia. (medscape.com)
  • Both progenitor cell populations responded to hypoxic stimuli by stabilizing the hypoxia inducible factor (HIF)-1alpha protein. (ox.ac.uk)
  • check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)
  • Short exposures to hypoxia increased the clonogenic myeloid capacity of UCB CD133(+) cells and promoted a significant increase in BMMC number. (ox.ac.uk)
  • The common stem cell produces 2 other stem cells, the myeloid stem cell and the lymphoid stem cell. (medscape.com)
  • BMMSCs showed significant protective effects on the ultrastructure of DCD donor livers and ROS-induced injury to IAR-20 cells under electron microscopy. (medscimonit.com)
  • BMMSCs also significantly improved the expression level of microtubule-associated protein 1 light chain 3 (LC3)-II in both DCD donor livers and ROS-induced injured IAR-20 cells, including upregulating the expression of ferritin. (medscimonit.com)