• How does osmotic modulation of the extracellular matrix affect the osteogenic differentiation of mesenchymal stroma cells (MSCs)? (charite.de)
  • We subsequently discuss how β-TCP can regulate osteogenic processes to aid bone repair/healing, namely osteogenic differentiation of mesenchymal stem cells, formation of blood vessels, release of angiogenic growth factors, and blood clot formation. (frontiersin.org)
  • To date, RUNX2 has been involved in diverse physiological processes, including osteogenic differentiation of mesenchymal stem/stromal cells, chondrocyte hypertrophy, immunomodulation, vascular invasion and endothelial cell migration via modulating a variety of signaling cascades (e.g. (spandidos-publications.com)
  • Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
  • Mesenchymal stem cells (MSCs), distributed in many tissues in the human body, are multipotent cells capable of differentiating in specific directions. (wjgnet.com)
  • It is usually considered that the differentiation process of MSCs depends on specialized external stimulating factors, including cell signaling pathways, cytokines, and other physical stimuli. (wjgnet.com)
  • Recent findings have revealed other underrated roles in the differentiation process of MSCs, such as material morphology and exosomes. (wjgnet.com)
  • However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
  • Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
  • We therefore briefly discuss differences found in subsets of human bmMSCs and in MSCs isolated from some other sources and touch upon how this could be utilized for cell-based therapies. (hindawi.com)
  • The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
  • Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
  • Overview of studies published regarding MSCs using the term "stem cell" or "stromal cell" in the last 20 years accessed by a web search in July 2015 (Google Scholar). (hindawi.com)
  • It seems that the term "stem cell" became more popular although the "stemness" was only shown in a more strict sense for MSCs involved in osteogenesis and bone repair. (hindawi.com)
  • When the biological properties of MSCs were explored in more detail, questions arose whether these cells met the criterion of a true stem cell [ 8 ]. (hindawi.com)
  • Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)
  • Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
  • Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
  • Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
  • MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
  • New treatment strategies have pursued the idea of using mesenchymal stromal cells (MSCs) in combination with osteoinductive materials to guide differentiation of MSCs into bone cells ensuring complete bone regeneration. (nature.com)
  • Furthermore, we revealed enhanced focal adhesion formation and osteogenic differentiation of MSCs cultured on positively charged protein fibers. (nature.com)
  • After bone fractures mesenchymal stromal cells (MSCs) take on a key role in the in vivo bone repair process 1 . (nature.com)
  • MSCs have the ability to differentiate into adipocytes and chondrocytes forming fat tissue and cartilage as well as into bone cells - called osteocytes. (nature.com)
  • The use of donor derived MSCs for triggering the bone regeneration in vivo is a promising tool for assisting the healing process. (nature.com)
  • Nevertheless, MSCs can lose their differentiation potential after expansion in vitro and transplantation into the site of injury 7 . (nature.com)
  • In order to cope with this task, in vitro systems are required which allow studies regarding cell-material interactions to unfold the impact of material surface properties on MSCs. (nature.com)
  • Interaction of MSCs with bone morphogenic proteins (BMPs) in an alginate scaffold mimicking the extracellular matrix (ECM) in vivo enhances their differentiation into bone cells 9 . (nature.com)
  • Mesenchymal stem cells (MSCs) are highly recommended for RA therapy because they can differentiate into various cell types and stimulate tissue repair. (hscn.org)
  • Mesenchymal Stem Cells (MSCs) specifically, are adult stem cells that are found in various tissues such as bone marrow, blood, dental tissue, among others. (hscn.org)
  • The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
  • Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. (techscience.com)
  • Thus, the expression of miR-103-3p decreases in MDS BM plasma and MDS-MSCs, significantly impacting MDS-MSCs differentiation. (techscience.com)
  • The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation, thereby providing possible target for the treatment of MDS pathogenesis. (techscience.com)
  • The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
  • On a cellular level, osmotic pressure influences cell fate decisions of mesenchymal stroma cells (MSCs). (charite.de)
  • Human bone marrow mesenchymal stem cells (hBM-MSCs) are the most commonly used cells. (biomedcentral.com)
  • The main challenge for large bone defect repair and regeneration remains the inadequate recruitment of mesenchymal stem cells (MSCs), reduced vascularization, and decreased growth factors stimulation within the scaffold construct to support cell viability and tissue growth. (frontiersin.org)
  • Cedars' research group successfully demonstrated the expression of the reporter hNIS in MSCs that had differentiated into bone-forming cells - demonstrating the ability to image stem cell differentiation long after the transfection of the reporter gene into the original MSC population. (ca.gov)
  • Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cell types depending on their environment, but cannot undergo neurogenesis in normal conditions. (aalto.fi)
  • In this thesis work, human MSCs (hMSCs) were differentiated into DA neurons on four different carbon-based materials and the differentiation process with and without differentiation factors was assessed by following markers related to neurogenesis. (aalto.fi)
  • In addition, the marker profiles revealed a positive effect of the nanostructures on the MSC differentiation, while PDL coating was found unfavorable for MSCs. (aalto.fi)
  • The study also showed that the adult OCRs are distinct from mesenchymal stem cells (MSCs), which play a role in bone generation during development and adulthood. (sciencedaily.com)
  • Researchers presumed that MSCs were the origin of all bone, cartilage, and fat, but recent studies have shown that these cells do not generate young bone and cartilage. (sciencedaily.com)
  • The CUMC study suggests that OCR stem cells actually fill this function and that both OCR stems cells and MSCs contribute to bone maintenance and repair in adults. (sciencedaily.com)
  • However, details regarding the regulation of MSC/MPC proliferation and differentiation status are still unclear and it is likely that regulation involves both biological and mechanical influences in the different environments. (scirp.org)
  • It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
  • Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
  • GR signaling controls genes required for differentiation of a subset of proliferative mesenchymal progenitors into matrix fibroblasts, in turn, regulating signals controlling AT2/AT1 progenitor cell proliferation and differentiation and identifying cells and processes by which glucocorticoid signaling regulates fetal lung maturation. (stanford.edu)
  • Integrin αvβ6 is found exclusively on epithelial cells. (wikipedia.org)
  • We have shown that AUF1 induces EMT and stemness in breast epithelial cells via stabilization of the SNAIL1 and TWIST1 mRNAs, and their consequent upregulation. (nature.com)
  • Ectopic expression of AUF1 also promoted stemness in mammary epithelial cells, and thereby increased the proportion of cancer stem cells. (nature.com)
  • These data prompted us to ask whether AUF1 upregulation could promote EMT as well as stemness in mammary epithelial cells. (nature.com)
  • Most human prostate cancers are adenocarcinomas, which originate from the epithelial cells that line the glands and ducts of prostate [ 2 ]. (jcancer.org)
  • Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
  • E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)
  • Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)
  • Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
  • Furthermore, E1A DNA and protein persisted in epithelial cells in the lungs of these animals 9 . (ersjournals.com)
  • Reports from several laboratories suggest that a phenomenon termed epithelial-mesenchymal transformation, in which epithelial cells transform into fibroblasts capable of producing connective tissue matrix, is an important feature of tissue remodelling 10 , 11 . (ersjournals.com)
  • The members of the EGF family are best known for their ability to stimulate cell growth and proliferation and are important for many developmental processes including promoting mitogenesis and differentiation of mesenchymal and epithelial cells. (rndsystems.com)
  • 2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
  • The purpose of this study was to track the differentiation profile and stress response of human bone marrow derived mesenchymal stem cells (hMSCs) undergoing osteogenic differentiation during exposure to a 20 mV/cm, 60 kHz electric field. (tufts.edu)
  • Real time reverse transcriptase-polymerase chain reactions (RT-PCR) were used to track osteogenic differentiation markers, namely alkaline phosphatase (ALP) and collagen type 1 (col1), and stress response markers, such as heat shock protein 27 (hsp27) and heat shock protein 70 (hsp70) Comparisons of collagen deposition between the stimulated hMSCs and controls were examined through second harmonic generation (SHG) imaging. (tufts.edu)
  • Human mesenchymal stem cells (hMSCs) have emerged as a leading candidate in cellular therapies due to their unique properties. (selectscience.net)
  • According to Korea Biomedical Review , the team found that nanoparticles displayed the potential to promote osteogenic differentiation (the process by which skeletal stem cells form, develop, repair, and maintain) of human mesenchymal (skeletal) stem cells (hMSCs) - adult cells obtained from bone marrow, not fetal cell lines - and subsequently enhance bone regeneration. (aleteia.org)
  • These experiments demonstrate various challenges related to developing an efficient protocol for DA differentiation from hMSCs, the most important being the optimization of the combined mechanical and chemical stimuli. (aalto.fi)
  • At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
  • They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
  • Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
  • Mesenchymal stem/progenitor cells (MSC/MPC) are found in many tissues and fluids including bone marrow, adipose tissues, muscle, synovial membranes, synovial fluid, and blood. (scirp.org)
  • Hart, D. (2014) Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
  • Ando, W., Heard, B., Nakamura, N., Frank, C.B. and Hart, D.A. (2012) Ovine Synovial Membrane-Derived Mescenchymal Progenitor Cells Retain the Phenotype of the Original Tissue That Was Exposed to in Vivo Inflammation: Evidence for a Suppressed Chondrogenic Differentiation Potential of the Cells. (scirp.org)
  • Loss of mesenchymal GR signaling blocked fibroblast progenitor differentiation into mature MFBs, which in turn increased proliferation of SOX9+ alveolar epithelial progenitor cells and inhibited differentiation of mature alveolar type II (AT2) and AT1 cells. (stanford.edu)
  • Cultured oligodendrocyte progenitor cells (OPCs) were incubated with HD ± BMSC-derived conditional medium (BMSC-CM). OPC differentiation was studied by immunostaining and morphometric analysis. (biomedcentral.com)
  • Before the actual treatment, the cells are then grown to about 100 million mesenchymal stem cells which we then convert into neural progenitor cells, cells which are committed to a neural lineage (WK note: neural progenitor cells are stem cells specific to the central nervous system). (wheelchairkamikaze.com)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • With this biomimetic in vitro model we identified the fibrous structure as well as the charge of the material to be responsible for its effects on MSC differentiation. (nature.com)
  • 2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
  • Towards a Mechanistic Model of Tau-Mediated Pathology in Tauopathies: What Can We Learn from Cell-Based In Vitro Assays? (ibecbarcelona.eu)
  • To enhance the osteogenic potential of stem cell sheet, we fabricated bone morphogenetic protein 2 ( BMP-2 ) gene-engineered cell sheet using a complex of polyethylenimine-alginate (PEI-al) nanocomposites plus human BMP-2 complementary(c)DNA plasmid, and studied its osteogenesis in vitro and in vivo. (dovepress.com)
  • The expression of osteogenesis-related gene was increased, which demonstrated that released BMP-2 could effectively induce the cell sheet osteogenic differentiation in vitro. (dovepress.com)
  • Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
  • Some of these techniques include novel tools for genetic manipulation, 4,5 approaches for in vitro disease modelling 6-9 and innovative co-culture system with autologous cell types 10,11 or bacteria, 12-14 as well as viral infection models. (stemcell.com)
  • Finally, the GM-I and Cedars-Sinai research team has developed a technology development strategy that translates the gamma microscope technology, useful for in vitro and in vivo mouse imaging, into future clinical applications involving the monitoring of stem cells and their progeny as well as the biological processes associated with the differentiation stages. (ca.gov)
  • In vitro cultured OPCs showed more differentiation after incubation with BMSC-CM. Hes1 expression was found to be significantly increased by HD and decreased by BMSC or BMSC-CM. The change of Hes1 was found, however, independent of Notch1 activation, but dependent on TNFα/RelB signaling. (biomedcentral.com)
  • In contrast, with the appropriate chemical and mechanical stimuli, these cells can be guided towards the neuronal lineage in vitro. (aalto.fi)
  • Here, using in vitro cell-based and in vivo mouse model experiments, we show that downregulation of O-GlcNAcylation, a key post-translational protein modification, promotes exosome secretion. (bvsalud.org)
  • Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)
  • The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
  • Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)
  • Mesenchymal Stromal Cell-Derived Extracellular Vesicles in Lung Diseases: Current Status and Perspectives. (cdc.gov)
  • TGF-β1 regulates multiple processes including cell proliferation, differentiation, angiogenesis, epithelial-mesenchymal-transition (EMT) and immune suppression. (wikipedia.org)
  • Integrins bind to specific extracellular proteins in the extracellular matrix or on other cells and subsequently transduce signals intracellularly to affect cell behaviour. (wikipedia.org)
  • Of note, binding regions for transcription factors STAT3 and C/EBPα were found, and basic normal cell expression of ITGB6 is thought to be regulated primarily by these proteins. (wikipedia.org)
  • Adipocyte-derived factors involved in tumor progression include proteins such as adiponectin, leptin, TNF-α, monocyte chemotactic protein-1 (MCP-1), IL-6, and ECM components that control tumor cell behavior within the tumor microenvironment. (jci.org)
  • Furthermore, proteins involved in epithelial-mesenchymal transition (EMT) were strongly altered, which plays a central role in metastasis. (spandidos-publications.com)
  • The transforming growth factor β (TGF-β) family is a large group of structurally related proteins that drive developmental programs and control cell behavior. (cshlpress.com)
  • In addition to accelerating the bone healing process per se, vitamin C is also important to the 1) formation of non-collagen bone matrix proteins, 2) differentiation of mesenchymal stem cells into bone cells, and 3) the regulation of cartilage-forming cells in the bone. (wemagazineforwomen.com)
  • Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)
  • These include lead's ability to inhibit or mimic the actions of calcium (which can affect calcium-dependent or related processes) and to interact with proteins (including those with sulfhydryl, amine, phosphate, and carboxyl groups) [ATSDR 2010]. (cdc.gov)
  • They include endothelial cells, fibroblasts, and keratinocytes. (frontiersin.org)
  • Our aim is to decipher this independent organisation of fibroblasts, vascular precursors, immune cells and mechanical instability in the complex environment of the tissue. (charite.de)
  • The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)
  • GR enhanced differentiation of a newly defined proliferative mesenchymal progenitor cell (PMP) into matrix fibroblasts (MFBs), in part by directly activating extracellular matrix-associated target genes, including Fn1, Col16a4, and Eln and by modulating VEGF, JAK-STAT, and WNT signaling. (stanford.edu)
  • ETP augmented fibrosis, angiogenesis, and inflammation through recruitment of macrophages and endothelial cells. (jci.org)
  • Deletion of the glucocorticoid receptor gene (Nr3c1) from fetal pulmonary mesenchymal cells phenocopied defects caused by global Nr3c1 deletion, while lung epithelial- or endothelial-specific Nr3c1 deletion did not impair lung function at birth. (stanford.edu)
  • ERG and CD31 immunoexpression prove endothelial differentiation. (medscape.com)
  • Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
  • In addition, they act as antigen presenting cells and promote angiogenesis [ 8 ]. (ersjournals.com)
  • These processes combine to heal wounds but when uncontrolled can promote tissue pathologies. (wikipedia.org)
  • Stem cell therapy for rheumatoid arthritis (RA) is a promising treatment that has the potential to reduce inflammation and promote tissue repair. (hscn.org)
  • One type of MSC based on its tissue source is umbilical cord mesenchymal stem cell (UC-MSC), which is believed to be the best among other types. (hscn.org)
  • These effects were partially mediated through enhanced TGF-β signaling, which contributes to tissue fibrosis and epithelial-mesenchymal transition (EMT) of tumor cells. (jci.org)
  • Similarly, CD34+ cells can be identified in human scar tissue, suggesting that these cells may contribute to tissue remodelling and repair [ 5 ]. (ersjournals.com)
  • Healing begins with the self-organisation of cells in the wound to reestablish structured tissue and restore the mechanical stability and intrinsic pretension of the injured matrix lost through the injury. (charite.de)
  • For this purpose, effect on cell and tissue level are investigated separately and combined. (charite.de)
  • Tissue hydration and water binding affects many processes in human physiology. (charite.de)
  • Bringing together cell and tissue level, we furthermore want to unravel how cell-matrix interactions are influenced by changing osmotic environments. (charite.de)
  • Tissue and organ regeneration is organized on the one hand by differentiated cells or mediated by the differentiation of stem cells along the individual cell type. (donau-uni.ac.at)
  • Mesenchymal stem cells are the stem cells of the connective tissue and thus the essential stem cells for the restoration of destroyed tissues and organs. (donau-uni.ac.at)
  • In this review, we introduce the broad application of β-TCP in tissue engineering and discuss the different approaches that β-TCP scaffolds are customized, including physical modification (e.g., pore size, porosity and roughness) and the incorporation of metal ions, other materials (e.g., bioactive glass) and stem cells (e.g., mesenchymal stem cells). (frontiersin.org)
  • Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
  • Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
  • Epithelial-mesenchymal transformation is now recognised as an important feature of tissue remodelling. (ersjournals.com)
  • Recently, stem cell sheet has been an emerging strategy in bone tissue engineering. (dovepress.com)
  • Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
  • Since the introduction of the mouse small intestinal organoid model in 2009, 1 there has been an avalanche of developments in this field, including development of culture conditions for human organoids derived from primary colonic tissue, 2 as well as from human pluripotent stem cells (hPSCs). (stemcell.com)
  • Here, we identify nonrandom distribution of a pathogenic mtDNA variant across a complex tissue using single-cell RNA-Seq, mitochondrial single-cell ATAC sequencing, and multimodal single-cell sequencing. (jci.org)
  • breathing, tissue settling, muscular motion, GM-I is developing correction techniques that follow the motions in real time and compensate for motion blurring of the cells under study. (ca.gov)
  • The researchers believe that OCR stem cells will be found in human bone tissue, as mice and humans have similar bone biology. (sciencedaily.com)
  • The researchers also suspect that OCR cells may play a role in soft tissue cancers. (sciencedaily.com)
  • Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
  • In this study, we used dorsomorphin, a selective small molecule inhibitor of BMP signaling, to investigate whether BMP signaling is involved in the positive effects of PPARγ agonists on osteogenic phenotypes of cultured human periosteum-derived cells. (medsci.org)
  • Although further study will be needed to clarify the mechanisms of PPARγ-regulated osteogenesis, our results suggest that the positive effects of a PPARγ agonist on the osteogenic phenotypes of cultured human periosteum-derived cells seem to be dependent on BMP signaling. (medsci.org)
  • Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)
  • It focuses on their ability to regulate immune responses and promote cell regeneration, citing a 2019 study that confirms their safety and effectiveness in reducing inflammation. (hscn.org)
  • Our results suggested that PEI-al nanocomposites efficiently deliver the BMP-2 gene to bone marrow mesenchymal stem cells and that BMP-2 gene-engineered cell sheet is an effective way for promoting bone regeneration. (dovepress.com)
  • 17 This property of rapid regeneration at intestinal stasis makes the intestine a uniquely convenient model system for epithelial cell biology and adult stem cell biology studies both inside and outside the specific context of intestinal function. (stemcell.com)
  • A team of researchers from the Catholic University of Korea School of Medicine recently revealed that they have been developing a method of stem cell therapy which allows for more efficient drug delivery at the cellular level, and it could also play a part in bone regeneration . (aleteia.org)
  • Dr Anand Srivastava: How stem cells help in regeneration? (medicaltourismco.com)
  • WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
  • No features of vascular differentiation, for example intracytoplasmic lumina or areas of conventional angiosarcoma, were observed. (medscape.com)
  • Malignant proliferation showing large monomorphic cells with some epithelioid (A1E/AE3) or angiocentric aspects without clear vascular differentiation. (medscape.com)
  • Stem cell therapy, on the other hand, is a medical treatment modality that employs the regenerative capacities of stem cells. (hscn.org)
  • Osteoblastic differentiation and stress response of human mesenchymal stem cells exposed to alternating current electric fields. (tufts.edu)
  • The differentiation of mesenchymal stem cells towards an osteoblastic fate depends on numerous signaling pathways, including activation of bone morphogenetic protein (BMP) signaling components. (medsci.org)
  • In our previous study, we demonstrated that an agonist of the perioxisome proliferator-activated receptor γ (PPARγ), a master regulator of adipocyte differentiation, stimulates osteoblastic differentiation of cultured human periosteum-derived cells. (medsci.org)
  • In addition, the PPARγ agonist clearly increased osteogenic differentiation medium-induced BMP-2 upregulation in the periosteum-derived osteoblastic cells at 2 weeks of culture as determined by quantitative reverse transcriptase polymerase chain reaction (RT-PCR), immunoblotting, and immunocytochemical analyses. (medsci.org)
  • Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)
  • Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
  • The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)
  • Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)
  • This evidence supports the potential use of laser-processed titanium surfaces as bone implant materials, and their use in regenerative medicine could promote better outcomes. (biomedcentral.com)
  • In addition to their regenerative ability, mesenchymal stem cells can also take on immunoregulatory abilities, although the exact mechanisms are unknown. (donau-uni.ac.at)
  • Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
  • The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
  • The Tisch Multiple Sclerosis Research Center of New York ( click here ) recently published the Phase 1 results of the first-ever FDA approved multiple sclerosis regenerative stem cell study ( click here ). (wheelchairkamikaze.com)
  • The next installment will include an overview of the upcoming FDA approved Phase 2 trial, as well as a discussion of how regenerative stem cells might work and their possible impact on the treatment landscape of multiple sclerosis. (wheelchairkamikaze.com)
  • 2 ] in 1994 and is defined by the expression of a variety of cell surface antigens, including the stem cell marker CD34, the pan-leukocyte marker CD45, and monocyte markers CD14 and CD11. (ersjournals.com)
  • This makes their identification and differentiation from resident mesenchymal cells extremely difficult in the absence of further specific markers. (ersjournals.com)
  • The behavior of mesenchymal stem cells from an umbilical cord vein was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a mineralization assay, and an alkaline phosphatase activity assay and by carrying out a quantitative real-time polymerase chain reaction for osteogenic markers. (biomedcentral.com)
  • The laser-processed titanium surface increased the proliferation of CHO-k1 cells, reduced the proliferation of mesenchymal stem cells, upregulated the expression of the osteogenic markers, and enhanced alkaline phosphatase activity. (biomedcentral.com)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • 2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)
  • It notes that the stem cells are ethically sourced from AATB-certified, US-donated, full-term human umbilical cords. (hscn.org)
  • Batsali, A.K., Kastrinaki, M.C., Papadaki, H.A. and Pontikoglou, C. (2013) Mesenchymal Stem Cells Derived from Wharton's Jelly of the Umbilical Cord: Biological Properties and Emerging Clinical Applications. (scirp.org)
  • Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
  • These characteristics make stem cells a suitable option for repairing damaged tissues and organs in the body. (hscn.org)
  • Even in the early stages of inflammatory processes, healing processes counteract the destruction of tissues and organs. (donau-uni.ac.at)
  • In recent years, epithelial-mesenchymal transition (EMT) has been suggested to play a key role in the process of embryonic development, differentiation of tissues and organs, chronic inflammation and fibrosis, as well as cancer progression ( 14 ). (spandidos-publications.com)
  • Borlongan, C.V., Glover, L.E., Tajiri, N., Kaneko, Y. and Freeman, T.B. (2011) The Great Migration of Bone Marrow-Derived Stem Cells towards the Ischemic Brain: Therapeutic Implications for Stroke and Other Neurological Disorders. (scirp.org)
  • Li, L. and Jiang, J. (2011) Regulatory Factors of Mesenchymal Stem Cell Migration into Injured Tissues and Their Signal Transduction Mechanisms. (scirp.org)
  • Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
  • Facial tissues, including lip and palate, are produced from mesenchymal migration, penetration, and fusion of cranioneural crest cells. (medscape.com)
  • Three paired anatomic processes are involved with this phenomenon of migration and penetration: (1) the medial nasal process coalesces with the (2) maxillary process, followed by the coalescence of the (3) lateral nasal process with the medial nasal process (see the image below). (medscape.com)
  • the decrease in O-GlcNAcylation levels was found to lead to the decreased proliferation, migration and invasion of EC cells. (bvsalud.org)
  • When germ-free cell cultures became a laboratory routine, hopes were high for using this novel technology for treatment of diseases or replacement of cells in patients suffering from injury, inflammation, or cancer or even refreshing cells in the elderly. (hindawi.com)
  • Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
  • According to a study by Castro-Manrreza and Montesinos2 published in 2015 shows that mesenchymal stem cells are known to regulate the immunity system, and improve the symptoms associated with MS by reducing the inflammation of the central nervous system and the loss of the myelin sheath (demyelination). (medicaltourismco.com)
  • During the process of airway inflammation, complex interactions of innate and adaptive immune cells as well as structural cells and their cytokines have many important roles. (cdc.gov)
  • Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)
  • In the present study, the hypothesis that adenoviral infection followed by persistent expression of its E1A gene is capable of producing epithelial-mesenchymal transformation was examined by investigating guinea pig PLECs that express adenoviral E1A. (ersjournals.com)
  • Morscheid S., Rey-Rico A., Schmitt G., Madry H., Cucchiarini M., Venkatesan J.K. Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-β and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells. (uniklinikum-saarland.de)
  • The TGF-β family members exert their effects by binding to receptors on the cell surface, activating intracellular signaling pathways that modulate gene expression programs that control normal cell physiology, immune responses, and a variety of developmental processes. (cshlpress.com)
  • PEI-al nanocomposites carrying BMP-2 gene could efficiently transfect bone marrow mesenchymal stem cells. (dovepress.com)
  • Recently, stem cell- and gene-based therapy has been applied in BTE and achieved some good clinical results. (dovepress.com)
  • Moreover, the combination of gene modification and directed differentiation of ESCs provides perfect tool for disease modelling and drug discovery. (benthamscience.com)
  • The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
  • We integrated genome-wide gene expression profiling, ATAC-seq, and single cell RNA-seq data in mice in which GR was deleted or activated to identify the cellular and molecular mechanisms by which glucocorticoids control prenatal lung maturation. (stanford.edu)
  • During aging, a subset of AT2 cells expressing the canonical Wnt target gene, Axin2, function as stem cells, renewing themselves while generating new AT1 and AT2 cells. (stanford.edu)
  • This "reporter gene" imaging technique works not only with the originally engineered cells but also with the progeny cells that result from splitting and differentiation of the stem cells. (ca.gov)
  • This is a dramatic improvement in the ability to visualize stem cells the hNIS gene stays with the descendents of the originally transfected cells and they will temporarily "shine" or become visible when 99mTc is injected into the body as a contrast tracer. (ca.gov)
  • This condition is inherited in an autosomal dominant pattern, which means one copy of the altered gene in each cell is sufficient to cause the disorder. (medlineplus.gov)
  • Gimble, J.M., Bunnell, B.A. and Guilak, F. (2012) Human Adipose-Derived Cells: An Update on the Transition to Clinical Translation. (scirp.org)
  • In human disease, the natural history of the remodelling process is probably much slower than in animal models. (ersjournals.com)
  • Stromal cells isolated from the PZ and TZ of normal human prostates mixed with DU145 cells subcutaneously injected into athymic nude mice. (jcancer.org)
  • The human body is made up of about 220 different kinds of specialized cells such as nerve cells, muscle cells, fat cells and skin cells. (benthamscience.com)
  • Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
  • The imaging of stem cell differentiation is possible by linking promoters - in this case a bone-forming promoter known as human Osteocalcin (hOc) - with the expression of hNIS. (ca.gov)
  • Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
  • As human osteosarcoma cells are a source of additional growth they did not affect osteoinduction. (bvsalud.org)
  • In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
  • Without this, the patient cells lost in PD could be replaced by grafted community is left trying to interpret complex scien- immature human dopaminergic neurons [3, 5]. (lu.se)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • Since epithelial-to-mesenchymal transition (EMT) and the acquisition of cancer stem cell traits are important for cancer onset and progression, we sought to determine the role of AUF1 in these two important processes. (nature.com)
  • The origins and basis for these fat deposits are largely unknown, but there is a possibility that the altered mechanical and biological environments lead to dysregulation of MSC/MPC and progression to preferential differentiation towards the adipocyte lineage. (scirp.org)
  • The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
  • Recent studies based on the National Institutes of Health-sponsored National Emphysema Treatment Trial have shown that the progression of COPD is associated with thickening of the walls of the small conducting airways by a repair and remodelling process 2 . (ersjournals.com)
  • For instance, RUNX2 is involved in the progression of various tumor types, such as osteosarcoma, renal cell carcinoma, gastric cancer and breast cancer ( 15 - 20 ). (spandidos-publications.com)
  • Of note, other studies have also indicated the involvement of RUNX2 in breast cancer stem cells (BCSCs) and breast cancer progression ( 22 , 23 ). (spandidos-publications.com)
  • Among factors that play direct role in initiation and progression of fibrotic process are epithelial-mesenchymal transition and myofibroblasts recruitment/differentiation, mediated by the transforming growth factor-beta1 (TGF-beta1). (cdc.gov)
  • However, more research is needed to determine the safety and efficacy of stem cell therapy for RA. (hscn.org)
  • Published in the November issue of Biomaterials , these groundbreaking findings represent the first successful attempt to use saponin-based nanoparticles in stem cell therapy. (aleteia.org)
  • This sustained intracellular drug delivery capability is important because it could help direct stem cell differentiation , a pivotal aspect of stem cell therapy that directs the pliant stem cells to turn into the desired cell. (aleteia.org)
  • We anticipate its broad application in various stem cell therapy domains in the coming years. (aleteia.org)
  • Stem Cell Therapy for Multiple Sclerosis in Mexico can help which is safe and effective. (medicaltourismco.com)
  • However, the stem cell therapy cost in Mexico for MS is nearly half as compared to its counterparts in the USA. (medicaltourismco.com)
  • Therefore, stem cell therapy for multiple sclerosis in Mexico can prove effective as it will not only stop further nerve cell degeneration but also promote regrowth of the damaged cells. (medicaltourismco.com)
  • Demyelinating conditions are difficult to treat and stem cell therapy using bone marrow mesenchymal stem cells (BMSCs) is a promising novel strategy. (biomedcentral.com)
  • Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
  • This pure protein-based and chemically modifiable, fibrous ECM model allows the investigation of MSC behavior on biomimetic materials to unfold new vistas how to direct cells' differentiation for the development of new bone regenerating strategies. (nature.com)
  • Through them, the cells modify their microenvironment and the behavior of neighboring cells. (frontiersin.org)
  • However, such cell behavior was investigated mainly in 2D cell culture experiments so far. (charite.de)
  • The laser-treated titanium surface modulated cellular behavior depending on the cell type, and stimulated osteogenic differentiation. (biomedcentral.com)
  • Gastrointestinal stromal tumors (GISTs) are a subset of GI mesenchymal tumors of varying differentiation. (medscape.com)
  • So it is no wonder that the protein p53, which works in our cells day and night to keep cancer at bay, has also been handed an important task in embryonic development. (weizmann.ac.il)
  • This finding emerged from research conducted by Dr. Eldad Tzahor of the Biological Regulation Department, whose expertise includes embryonic development, and Prof. Varda Rotter of the Molecular Cell Biology Department, a pioneer of p53 research. (weizmann.ac.il)
  • But they may also have relevance for cancer studies: "We have known for a long time that cancer processes are similar to those of embryonic development, only deregulated and uncontrolled," says Tzahor. (weizmann.ac.il)
  • To further test the osteogenic potential of the cell sheet in vivo, enhanced green fluorescent protein or BMP-2-producing cell sheets were treated on the cranial bone defects. (dovepress.com)
  • These organoids include a polarized epithelial layer that surrounds a functional lumen and contains cell types of the intestinal epithelium present in proportions and relative spatial arrangement that mimic what is observed in vivo. (stemcell.com)
  • Niche Cells and Signals that Regulate Lung Alveolar Stem Cells In Vivo. (stanford.edu)
  • Here, we review alveolar renewal and repair with a focus on the niches, rather than the stem cells, highlighting what is known about the cellular and molecular mechanisms by which they control stem cell activity in vivo. (stanford.edu)
  • A Novel SPECT microscopy system for 3D imaging of single stem cells in vivo Individual stem cells can be visualized within the body - "in vivo" by genetically engineering the cells to absorb a contrast agent when the agent is present within the body. (ca.gov)
  • and 2) development of new imaging hardware, a gamma microscope, to visualize the individual stem cells in vivo. (ca.gov)
  • The research group at Gamma Medica-Ideas is responsible for developing the microscope that is capable of visualizing individual stem cells in vivo. (ca.gov)
  • BMP signaling also has a critical role in the processes by which mesenchymal stem cells undergo commitment to the adipocyte lineage. (medsci.org)
  • During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
  • Before their rapid differentiation, these cells undergo another process, called epithelial mesenchymal transition (EMT), which enables them to begin migrating from their original site in the neural tube - the embryonic template of the central nervous system - to the facial area. (weizmann.ac.il)
  • This key process is similar in many respects to processes that cancer cells undergo when they become malignant. (weizmann.ac.il)
  • Heterotopic ossification (HO) is a dynamic, complex pathologic process that often occurs after severe polytrauma trauma, resulting in an abnormal mesenchymal stem cell differentiation leading to ectopic bone growth in soft-tissues including tendons, ligaments, and muscles. (nih.gov)
  • in contrast, abnormal tau deposition in neurons and glial cells occurs only exceptionally in children. (ibecbarcelona.eu)
  • The wide range of manifestations observed in patients with mitochondrial disease results from varying fractions of abnormal mtDNA molecules in different cells and tissues, a phenomenon termed heteroplasmy. (jci.org)
  • Embryology processes of abnormal palatal development. (medscape.com)
  • Therefore, it is important to understand how MSC differentiation into bone cells is controlled and how this process can be guided or improved by externally added factors. (nature.com)
  • The study's findings hint that p53 links two parallel developmental processes - cell division/proliferation and EMT. (weizmann.ac.il)
  • BioTek's Cytation™ 5 Cell Imaging Multi-Mode Reader was used to monitor spheroids in the inserts during the differentiation process using the brightfield channel, as well as to capture fluorescent images to verify protein marker expression and chondrocyte differentiation. (selectscience.net)
  • Zhou JQ, Wan HY, Wang ZX, Jiang N. Stimulating factors for regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells. (wjgnet.com)
  • Traditional BTE involves three-dimensional biodegradable scaffolds, stem cells, and osteoinductive growth factors. (dovepress.com)
  • The differentiation medium was a cocktail of BDNF, sonic hedgehog (Shh) and fibroblast growth factors (FGF2 and FGF8). (aalto.fi)
  • Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
  • LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
  • What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)
  • Importantly, breast cancer cells that ectopically express AUF1 were more efficient in forming orthotopic tumor xenografts in nude mice than their corresponding controls with limiting cell inocula. (nature.com)
  • Adipocytes represent a major cell type in the mammary tumor microenvironment and are important for tumor growth. (jci.org)
  • Our results highlight the crucial role of ETP as an obesity-associated factor that promotes tumor growth in the context of adipocyte interactions with tumor and stromal cells. (jci.org)
  • The recurrence and drug resistance of breast cancer are intractable due to the presence of breast cancer stem cells (BCSCs), which are adequate to initiate tumor formation and refractory to conventional remedies. (spandidos-publications.com)
  • Gastric gastrointestinal stromal tumor (GIST) en face. (medscape.com)
  • Tumor cells were arranged in solid sheets [Fig. 2(A)]. Numerous mitosis and necrosis were observed. (medscape.com)
  • American journal of respiratory cell and molecular biology 2022 0 0. (cdc.gov)
  • The study overexpressed PD-L1 by constructing a recombinant of PD-L1-lentiviral vector, which was subsequently used to transfect mouse bone marrow mesenchymal stem cells (MBMMSCs) and significantly suppressed the development of collagen-induced arthritis (CIA) in DBA/1j mice. (hscn.org)
  • The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. (frontiersin.org)
  • The exosome vesicles are a type of extracellular vesicles (EV), which are defined as lipid-bilayer spheroid structures, without replicating capacity, that are released from cells, including both prokaryotes and eukaryotes. (frontiersin.org)