Mesenchymal differentiation mesenchymal stem cell transplantation. Medical search. Frequent questions

There has been an increasing interest in recent years in the stromal cell system functioning in the support of hematopoiesis. (nih.gov)
The stromal cell system has been proposed to consist of marrow mesenchymal stem cells that are capable of self-renewal and differentiation into various connective tissue lineages. (nih.gov)
Data from preclinical transplantation studies suggested that stromal cell infusions not only prevent the occurrence of graft failure, but they have an immunomodulatory effect. (nih.gov)
Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)
Overview of studies published regarding MSCs using the term "stem cell" or "stromal cell" in the last 20 years accessed by a web search in July 2015 (Google Scholar). (hindawi.com)
Effects of hematopoietic stem cell adhesion on marrow stromal cell cytokine p. (rochester.edu)
Mesenchymal stem cells (MSCs) are an adult stromal cell population possessing powerful differentiation capacity and a prospect of use across main histocompatibility complicated barriers. (biongenex.com)
Human mesenchymal stromal cell transplantation modulates neuroinflammatory milieu in a mouse model of amyotrophic lateral sclerosis. (jamanetwork.com)

However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
Chemical compounds that modulate atrial and ventricular cell fate could be used to improve subtype-specific differentiation of endogenous or exogenously delivered progenitor cells in order to promote cardiac regeneration. (biomedcentral.com)
Collectively, these results indicate the potential for therapeutic alteration of cell fate decisions and pathological gene regulatory networks by GATA4-targeted compounds modulating chamber-specific transcriptional programs in multipotent cardiac progenitor cells and cardiomyocytes. (biomedcentral.com)
Thus, small molecules capable of influencing the fate decisions and differentiation programs of multipotent progenitor cells could facilitate therapeutic regeneration of lost myocardium. (biomedcentral.com)
MesenCult™ Adipogenic Differentiation Medium (Human) is specifically formulated for the in vitro differentiation of primary human mesenchymal stromal cells, and hPSC-derived mesenchymal progenitor cells (also known as mesenchymal stem cells or MSCs) into adipogenic lineage cells. (stemcell.com)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

The differentiation, proliferation, prognosis, and safety of MSCs for in vivo therapy remain controversial. (molvis.org)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
We therefore briefly discuss differences found in subsets of human bmMSCs and in MSCs isolated from some other sources and touch upon how this could be utilized for cell-based therapies. (hindawi.com)
The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
It seems that the term "stem cell" became more popular although the "stemness" was only shown in a more strict sense for MSCs involved in osteogenesis and bone repair. (hindawi.com)
When the biological properties of MSCs were explored in more detail, questions arose whether these cells met the criterion of a true stem cell [ 8 ]. (hindawi.com)
Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)
Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
Transplantation of mesenchymal stem cells (MSCs) may offer a viable treatment for Huntington's disease (HD). (allergy-link.com)
On day 28, rats in all groups except Sham and 3NP, received intrastriatal injections of either 200,000 MSCs (TP(low)), 400,000 (TP(high)) MSCs or DMEM (VH, the vehicle for transplantation). (allergy-link.com)
Human orofacial bone-derived mesenchymal stem cells (OMSCs) showed distinct differentiation traits from mesenchymal stem cells (MSCs) derived from long bones, mouse OMSCs have not been isolated due to technical difficulties, which in turn precludes using mouse models to study orofacial diseases. (ca.gov)
This kit is suitable for the differentiation of MSCs derived from human bone marrow (BM), adipose tissue, umbilical cord, or pluripotent stem cells (PSCs) that have been previously culture-expanded in serum- and animal component-free medium (e.g. (stemcell.com)
Adipogenic differentiation of human bone marrow-derived MSCs using MesenCult™ Adipogenic Differentiation Medium (Human) or a competitor medium. (stemcell.com)
The aim of our study was to determine the fate of injected mesenchymal stem cells (MSCs) and whole bone marrow (BM) cells in the infarcted heart. (ox.ac.uk)
MSCs were purified from BM of transgenic mice and characterized using flow cytometry and in vitro differentiation assays. (ox.ac.uk)
Myocardial infarctions were generated in mice and different cell populations including transgenic MSCs, unfractionated BM cells, or purified hematopoietic progenitors were injected. (ox.ac.uk)
While multipotent mesenchymal stromal cells have been recently isolated from adult lung (L-MSCs), there is very limited data on their biological properties and therapeutic potential in vivo. (nih.gov)
In this study, we characterized L-MSC phenotype, clonogenicity, and differentiation potential, and compared L-MSCs to BM-MSCs in vivo survival, retention, paracrine gene expression, and repair or elastase injury after transplantation. (nih.gov)
Long after transplantation (4 or 32 days), a significantly higher number of CD45(neg) L-MSCs were retained than BM-MSCs. (nih.gov)
This study demonstrates that tissue-specific L-MSCs possess mechanisms that enhance their lung retention after intravenous transplantation, and produce substantial healing of elastase injury comparable to BM-MSCs. (nih.gov)
Because of the immunological difficulties associated with allogeneic transplantation mesenchymal stem cells (MSCs) have the potential to be an attractive tissue replacement therapy for a number of reasons. (biongenex.com)
MSCs are multipotent cells that can be readily isolated from a number of adult tissues including bone marrow umbilical cord blood adipose tissue and placenta. (biongenex.com)
They have been well documented to differentiate into osteogenic adipogenic and chondrogenic lineages by activation of the cells through encountering inflammatory cytokines such as IFN-γ [21 23 Allogeneic MSCs from young healthy donors are an attractive source of regenerative cells for the treatment of degenerative diseases with an inflammatory component. (biongenex.com)
As MSCs possess potent immunomodulatory properties and an ability to differentiate into several lineages there is potential for allogeneic 'off the shelf' tissue engineering solutions using these cells. (biongenex.com)
With the increasing number of clinical trials utilising allogeneic MSCs for acute and chronic diseases a comprehensive knowledge of the influence of differentiation in the immunological account of MSCs is vital. (biongenex.com)
Adult stem cells known as mesenchymal stem cells (MSCs) can be found in bone marrow, adipose tissue (fat), and umbilical cord tissue, among other organs. (suzermedclinic.com)
Most studies regarding the marrow-derived equine mesenchymal stem cells (MSCs) have mainly focused on the cell transplantation without considering the capacity of differentiation and in vitro requirements of the cells. (ac.ir)
According to our findings, equine MSCs were easily generated specialized bone, cartilage and adipose cell lineages as confirmed by specific staining and RT-PCR analysis. (ac.ir)
In summary, marrow-derived equine MSCs possess tripotent differentiation capacity and rapid growth rate in the appropriate culture conditions. (ac.ir)

Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
For instance, the bone marrow's hematopoietic stem cells may give birth to several types of blood cells. (suzermedclinic.com)
For instance, mesenchymal stem cells can develop into bone, cartilage, and fat cells, while hematopoietic stem cells in the bone marrow can produce several types of blood cells. (suzermedclinic.com)
Support for the immunological System: Specific stem cells, including hematopoietic stem cells found in the bone marrow, are in charge of producing various blood cells, including white blood cells that are essential for the immunological response. (suzermedclinic.com)

Human embryonic stem cells also exhibit the Warburg effect ( 15 ). (spandidos-publications.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Embryonic stem cells: Only a few days old embryos are the source of these stem cells. (suzermedclinic.com)
Due to their adaptability, embryonic stem cells are useful for both scientific study and possible medicinal applications. (suzermedclinic.com)
Embryonic stem cells (ESCs): These stem cells are commonly collected through in vitro fertilization (IVF) clinics and are derived from embryos at the blastocyst stage. (suzermedclinic.com)
Because embryonic stem cells are pluripotent, they can develop into any form of cell in the body. (suzermedclinic.com)
Scientists may "reprogram" mature cells, such skin cells, to behave like embryonic stem cells by inserting particular genes. (suzermedclinic.com)
The advantage of iPSCs is that they do not raise the ethical issues raised by embryonic stem cells. (suzermedclinic.com)

HADSCs were induced to confirm that they can express the characteristics of endothelial cells (ECs) in vitro. (molvis.org)
2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
Another example is repairing bone defects using mesenchymal stem cells and mesenchymal-derived endothelial cells. (kean.edu)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
Although patent channels were identified at 8 weeks, no evidence suggested that the channels had developed an endothelial cell lining, thus confirming successful rearterialization. (medscape.com)

Promotes osteogenic, adipogenic, and chondrogenic differentiation of human mesenchymal cells (Jaiswal et al. (stemcell.com)
Promotes osteogenic, adipogenic, and chondrogenic differentiation of mouse mesenchymal cells (Tropel et al. (stemcell.com)
Passage-2 equine MSC cultures were then treated with appropriate supplements in order to examine the cell osteogenic, chondrogenic and adipogenic differentiation potential. (ac.ir)

born February 1970) is the chairmen of Stem Cell Transplantation Center (also called as Department of Neural Stem Cells) in the General Hospital of Chinese People's Armed Police Forces. (wikipedia.org)
Differentiation of rat embryonic neural stem cells promoted by co-cultured Schwann cells. (wikipedia.org)
Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke. (wikipedia.org)
Differentiation of embryonic neural stem cells promoted by co-cultured Schwann cells [J]." Chinese Journal of Neurosurgery 2 (2002). (wikipedia.org)
A single cell-based approach to characterize human induced pluripotent stem cells resistant to differentiation finds cellular properties of neural and mesenchymal origins, pointing at underlying causes for undesired grafts after transplantation. (nature.com)
Differentiation of new neurons from neural stem cells is described. (kean.edu)
these cells can induce neural differentiation. (kean.edu)
Anderson, L. and Caldwell, M.A. (2007) Human neural progenitor cell transplants into the subthalamic nucleus lead to functional recovery in a rat model of Parkinson's disease. (scirp.org)
A two-year follow-up study of co-transplantation with neural stem/precursor cells and mesenchymal stromal cells in ischemic stroke patients. (stemcellclinicsofamerica.net)
Intrathecal transplantation of human neural stem cells overexpressing VEGF provide behavioral improvement, disease onset delay and survival extension in transgenic ALS mice. (stemcellclinicsofamerica.net)
The nervous system contains neural stem cells, which are largely located in particular parts of the brain and spinal cord. (suzermedclinic.com)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
ARPE-19 cells at passages p22 to p28 were cultured on filters for up to 3 months in DMEM/F12 or DMEM media with or without pyruvate and 1% fetal calf serum . (allergy-link.com)
Furthermore, the culture of the cells was investigated in terms of the optimal concentration of fetal bovine serum (FBS) and the initial cell-seeding density. (ac.ir)

The results showed that MSC did not alter their osteogenic differentiation capacity, surface marker profile, and the expression profiles of MSC during expansion. (biomedcentral.com)
Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. (biomedcentral.com)
Finally we analyzed the gene expression profiles of MSC during osteogenic differentiation. (biomedcentral.com)
The formulation, licensed from Plasticell, produces more consistent and potent osteogenic differentiation than currently available formulations, enabling a more reproducible, efficient method for creating bone tissue and advancing research in bone disease and healing. (plasticell.co.uk)

We developed techniques to isolate mouse OMSCs derived from mandibles and verified their MSC characteristics by single colony formation, multi-lineage differentiation, and in vivo tissue regeneration. (ca.gov)
Serial neurosphere culture, multi-lineage differentiation, and orthotopic transplantation were used to assess whether these mutations induced de-differentiation of cortical astrocytes into glioma stem cells. (nih.gov)
Results: G1/S-defective, Rb knockout astrocytes gained unlimited self-renewal and multi-lineage differentiation capacity, in both the presence and absence of Kras and Pten mutations. (nih.gov)
A fraction of ADIPOSE TISSUE prepared to enrich in STEM CELLS with the capacity for multi-lineage differentiation. (bvsalud.org)

Recent efforts demonstrated that the multiple mesenchymal lineages can be clonally derived from a single mesenchymal stem cell, supporting the proposed paradigm. (nih.gov)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
However, current approaches for chondrogenic differentiation of hiPSCs are complicated and inefficient primarily due to intermediate embryoid body formation, which is required to generate endodermal, ectodermal, and mesodermal cell lineages. (mdc-berlin.de)

To qualify as stem cells, self-renewal has to be shown as well [ 10 ]. (hindawi.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
Human mesenchymal stem cells (HuMSC) have low immunogenicity, self-renewal, and are easy to obtain, making it a promising therapy for liver regeneration [ 4 ]. (bsmiab.org)
We isolated a new population of stem cells from human orofacial tissue gingiva, a tissue source easily accessible from the oral cavity, namely GMSC, which exhibited clonogenicity, self-renewal, and multipotent differentiation capacities. (ca.gov)
In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)

With other REMEDI researchers we are specifically studying the immune therapeutic properties of mesenchymal stem cells (MSC). (universityofgalway.ie)
Transplanting their own (autologous) bone marrow-derived stem cells into 48 patients with end-stage liver disease resulted in therapeutic benefit to a high number of the patients, report researchers publishing in the current issue of Cell Transplantation (19:11). (medicaldaily.com)
With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
In recent years mesenchymal stem cells (MSC) have generated a great deal of interest as a potential source for cell-based therapeutic strategies. (biomedcentral.com)
Cultured retinal pigment epithelium (RPE) may become a therapeutic option for transplantation in retinal disease. (allergy-link.com)
Use of stem cell lines in therapeutic applications is discussed. (kean.edu)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
Morscheid S., Rey-Rico A., Schmitt G., Madry H., Cucchiarini M., Venkatesan J.K. Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-β and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells. (uniklinikum-saarland.de)
Stem cell therapy is an emerging therapeutic modality in the treatment of stroke. (stemcellclinicsofamerica.net)
With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)

Human mesenchymal stem cell-based therapy can help liver regeneration directly, through hepatogenic differentiation, or indirectly through the paracrine secretome. (bsmiab.org)
However, studies have found that UDCA can inhibit DNA repair mediated by poly (ADP-ribose) polymerase, thereby interfering with the effectiveness of regeneration of the damaged cells. (bsmiab.org)
Secretome is a soluble molecule and exosome produced by HuMSC, which can support hepatic epithelial regeneration through hepatogenic differentiation. (bsmiab.org)
One of the key objectives for bone regeneration therapy to be successful is to direct stem cells proliferation and to accelerate their differentiation in a controlled manner through the use of growth factors and osteogenic inducers. (elsevierpure.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
Schwann cells (SCs) have important roles in development, myelination and regeneration in the peripheral nervous system. (scirp.org)
Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for hyaline cartilage regeneration. (mdc-berlin.de)
The body's normal repair and regeneration processes depend heavily on stem cells. (suzermedclinic.com)
Stem cells have the capacity to replace ill or damaged body cells in tissue repair and regeneration. (suzermedclinic.com)

Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)

The ideal treatment option for terminally ill patients is organ transplantation. (frontiersin.org)
Organ transplantation is the ultimate treatment option for patients suffering from refractory diseases. (frontiersin.org)
Depending on the patient's medical condition, a refractory disease patient also requires an on-time selective option, such as less invasive cellular therapy options or curative organ transplantation that can function immediately after transplantation. (frontiersin.org)
I developed specific interest in the immune-modulating properties of Vitamin D, the molecular basis for chronic kidney transplant rejection, complications of organ transplantation and the involvement of cells from the immune system in kidney disease. (universityofgalway.ie)
Introduction Body Vancomycin organ transplantation being a medical procedure to displace a broken or defective body organ continues to be performed for over 100?years. (biongenex.com)

The OsteoMAX-XF™ differentiation media adds to EMD Millipore's comprehensive portfolio of mesenchymal stem cell products including primary human and rat mesenchymal stem cell lines, expansion media, antibodies, characterization kits, growth factors and ECM proteins. (plasticell.co.uk)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)

2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
Conclusion: A defined set of core signaling pathway mutations induces de-differentiation of cortical murine astrocytes into glioma stem cells. (nih.gov)
Systemic treatment with adipose-derived mesenchymal stem cells ameliorates clinical and pathological features in the amyotrophic lateral sclerosis murine model. (jamanetwork.com)
Neurogenic differentiation of murine and human adipose-derived stromal cells. (stemcellclinicsofamerica.net)

Under oxidative stress conditions, hMSCs can promote protective autophagy and reduce cell apoptosis or the level of apoptosis caused by excessive autophagy. (researchsquare.com)
This experiment intends to activate the oxidative stress response by H2O2, regulate the autophagy level of human bone mesenchymal stem cells (hBMSCs), and observe the effect of autophagy on the proliferation and apoptosis of hMSCs under oxidative stress. (researchsquare.com)
Human mesenchymal stem cells (hMSCs) is viable for xenogenic transplantation. (ejournals.ca)
Here we show that graphene provides a promising biocompatible scaffold that does not hamper the proliferation of human mesenchymal stem cells (hMSCs) and accelerates their specific differentiation into bone cells. (elsevierpure.com)

This study optimized conditions for the differentiation of hONPs towards dopaminergic neurons. (scirp.org)
They have the capacity to develop into the three cell types necessary for the maintenance and repair of the nervous system: neurons, astrocytes, and oligodendrocytes. (suzermedclinic.com)
In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
Several academic and industry efforts are well under owned subsidiary Cyto Therapeutics, it had received way to produce dopaminergic neurons from stem approval by the Australian government to conduct a cells under conditions compliant with use in patients. (lu.se)
Without this, the patient cells lost in PD could be replaced by grafted community is left trying to interpret complex scien- immature human dopaminergic neurons [3, 5]. (lu.se)

We report a new, straightforward and highly efficient approach for chondrogenic differentiation of hiPSCs, which avoids embryoid body formation. (mdc-berlin.de)

Preclinical and early clinical safety studies are paving the way for further applications of mesenchymal stem cells in the field of transplantation with respect to hematopoietic support, immunoregulation, and graft facilitation. (nih.gov)
Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
However, clinical practice and experiments have shown that ischemia, hypoxia, and inflammatory cell infiltration at the injured site will produce a large amount of ROS, forming an oxidative stress microenvironment. (researchsquare.com)
My interest in transplantation resulted in a research fellowship in basic immunology at the University of Chicago, USA following which I spent 9 years combining clinical transplant medicine with laboratory-based immunology research at Mayo Clinic. (universityofgalway.ie)
9. Clinical complications and outcomes of human kidney and pancreas transplantation. (universityofgalway.ie)
Cholestasis is a clinical syndrome caused by reduced bile secretion from liver cells, impaired bile secretion at the cholangiocyte level, obstruction of bile flow by stones (cholelithiasis), or tumor masses. (bsmiab.org)
Cell-based therapy using systemic infusion of GMSC in experimental colitis significantly ameliorated both clinical and histopathological severity of the colonic inflammation, restored the injured gastrointestinal mucosal tissues, reversed diarrhea and weight loss, and suppressed the overall disease activity in mice. (ca.gov)
This study demonstrated clinical and experimental evidences supporting a potential efficacy and safety of utilizing autologous PDL cells in the treatment of human periodontitis. (ca.gov)
This would be a useful clinical cell source. (cellsurgicalnetwork.com)
Induction of nucleus pulposus cells by autogenous mesenchymal stem cells also would be an important subject for a clinical trial. (cellsurgicalnetwork.com)
Clinical application of the cells derived from a human nucleus pulposus cell line is an important project to be undertaken in the near future. (cellsurgicalnetwork.com)
Stem Cells (ADSC's) for clinical research and deployment. (stemcellclinicsofamerica.net)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)
A second press release [2] has indicated stem cell source, resulting in widespread excitement that the program is planning to move forward very rapidly, with all of the patients being enrolled in the Correspondence to: Roger A. Barker, John van Geest Centre first quarter of 2016 and interim results being shared for Brain Repair, Department of Clinical Neurosciences, Univer- in October 2016. (lu.se)

Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
Previously, we showed that exosomes derived from human adipose tissue-derived mesenchymal stem cells (ASC-exosomes) attenuate AD-like symptoms by reducing multiple inflammatory cytokine levels. (mdpi.com)
One great advantage of MSC is that these cells may be directly obtained from individual patients, thereby eliminating the complications associated with immune rejection of allogenic tissue and infectious diseases. (biomedcentral.com)
The purpose of this study was to fabricate and evaluate the osteogenic potential diferentiation of human amnion mesenchymal stem cell with carbonate apatiteâ€"chitosan scaffolds (CA-ChSs) for tissue engineering. (ejournals.ca)
Current tissue engineering approaches combine different scaffold materials with living cells to provide biological substitutes that can repair and eventually improve tissue functions. (elsevierpure.com)
We conclude that the developmental fate of BM-derived cells is not restricted by the surrounding tissue after myocardial infarction and that the MSC fraction underlies the extended bone formation in the infarcted myocardium. (ox.ac.uk)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Critical to the hypothesis is the experimental fact that human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of nerve tissue engineering. (scirp.org)
These novel differentiated cells in fibrin gel might open new opportunities for the management of cell survival and neurotrophic potential in tissue engineering approaches for nerve repair. (scirp.org)
A unique class of cells in the body called stem cells has the extraordinary capacity to differentiate into many cell and tissue types. (suzermedclinic.com)
Adult stem cells have the capacity to develop into a variety of cell types relevant to the tissue or organ in which they are found. (suzermedclinic.com)
They can stimulate tissue repair, replace unhealthy or damaged cells, and aid in the growth and development of organs during embryonic development. (suzermedclinic.com)
Adult stem cells also assist in the continual preservation and regrowth of several bodily tissues, maintaining tissue homeostasis. (suzermedclinic.com)
Pulp cells cultures were established by two approaches: enzyme digestion (3 mg/mL type I colagenase and 4 mg/mL dispase), or culture of the tissue explants in cell culture dishes. (bvsalud.org)
Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. (bvsalud.org)

Blood cells from these patients show defective induction of specific subsets of genes after exposure to microbial stimuli in vitro . (nature.com)
Expression of Pdx-1, Glut-2 and Insulin genes in EX-4 treated cells was significantly higher than the cells exposed to differentiation media without EX-4. (celljournal.org)
Transcription factor GATA4-targeted compounds that have previously shown in vivo efficacy in cardiac injury models were tested for stage-specific activation of atrial and ventricular reporter genes in differentiating pluripotent stem cells using a dual reporter assay. (biomedcentral.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)

Does Transplantation Induce Aging In Patients? (medicaldaily.com)
These cytokines induce caspase-3 activation as an effector caspase that activates hepatic stellate cells (HSCs) and cause fibrogenesis [ 11 ]. (bsmiab.org)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)

We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application. (molvis.org)
After induction, hADSCs expressed von Willebrand Factor (vWF), the cell marker of ECs. (molvis.org)
Therefore, in this study, we assessed the ability of human adipose mesenchymal stem cells (hADSCs) to express the characteristics of ECs after induction in vitro to evaluate the theoretical feasibility of maintaining or restoring the function of ECs after intraocular application. (molvis.org)
Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19. (allergy-link.com)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
In this study, we investigate the expression of differentiated Schwann cell markers by exposing of endometrial stem cells with induction media including FGF2/FSK/HRG/RA. (scirp.org)

Mesenchymal stem cell transplantation and DMEM administration in a 3NP rat model of Huntington's disease: morphological and behavioral outcomes. (allergy-link.com)

Immunocytochemistry testing revealed that the target cells expressed strong mesenchymal stem cell marker CD 105. (ejournals.ca)
Using immunocytochemistry, we show that differentiated cells express S100 and P75 markers. (scirp.org)
The cells were cultured under morphological observations, differentiation tests and immunocytochemistry staining. (ac.ir)
Results: Morphological observations and, differentiation and immunocytochemistry tests conformd that the isolated cells were mesenchymal stem cells. (ac.ir)

To observe the effect of H2O2 induced oxidative stress on autophagy and apoptosis of human bone marrow mesenchymal stem cells (hBMSCs). (researchsquare.com)
The secretome was also found to inhibit cell apoptosis [ 6 ], suggesting that adipose-derived stem cell secretome (ADSC-S) can reduce liver cell apoptosis within 1-3 days after surgery. (bsmiab.org)
Because of this, we aimed to study the effect of human mesenchymal stem cell secretome (HuMSC-S) administration on caspase 3 levels and apoptosis of hepatocytes in a rat model with cholestasis after choledochal duct ligation receiving standard UDCA therapy. (bsmiab.org)
The power of the thiol proteinases to cleave a multitude of substrates in response to calcium mineral activation allows their involvement in a variety of cell processes offering cell motility, sign transduction, apoptosis, cell differentiation and legislation of the cytoskeleton (3). (exposed-skin-care.net)
The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)

Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. (bvsalud.org)

4. Stem cell and regenerative medicine related immunology. (universityofgalway.ie)
Stem cells and their potential for regenerative medicine, including the treatment of illnesses, injuries, and hereditary problems, are now the subject of ongoing research. (suzermedclinic.com)

Transplantation of human bone marrow-derived mesenchymal stem cells promotes behavioral recovery and endogenous neurogenesis after cerebral ischemia in rats. (wikipedia.org)
Recent data from in vitro models demonstrating the essential role of stromal support in hematopoiesis shaped the view that cell-cell interactions in the marrow microenvironment are critical for normal hematopoietic function and differentiation. (nih.gov)
The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
MSC mediated bone formation involves in both donor and recipient cells, but only recipient cells contribute to marrow element formation. (ca.gov)
The method for isolating bone marrow stromal cells is explained. (kean.edu)
Potential risks of bone marrow cell transplantation into infarcted hearts. (ox.ac.uk)
Background: This study proposed to examine the possibility of homing of bone marrow derived-mesenchymal stem cells after allograft transplantation in irradiated and healthy bone marrows. (ac.ir)
Mesenchymal stem cells were isolated from femoral bone marrow of male rats. (ac.ir)
PCR results for the healthy and irradiated rats in all time gaps after transplantation were negative indicating that male mesenchymal stem cells were failed to home in bone marrow of transplanted female rats. (ac.ir)
Sadan O, Melamed E, Offen D. Bone-marrow-derived mesenchymal stem cell therapy for neurodegenerative diseases. (jamanetwork.com)

It was suggested that lactate promoted the survival of iPS cells cultured in a medium without glucose and supplemented with galactose. (spandidos-publications.com)
Glucose is essential for cell survival and is metabolized to pyruvate ( 13 ). (spandidos-publications.com)
Sadan O, Melamed E, Offen D. Intrastriatal transplantation of neurotrophic factor-secreting human mesenchymal stem cells improves motor function and extends survival in R6/2 transgenic mouse model for Huntington's disease. (jamanetwork.com)
Intravenous mesenchymal stem cells improve survival and motor function in experimental amyotrophic lateral sclerosis. (jamanetwork.com)
Human mesenchymal stem cell transplantation extends survival, improves motor performance and decreases neuroinflammation in mouse model of amyotrophic lateral sclerosis. (stemcellclinicsofamerica.net)

However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
The differentiation rate is comparable to the one achieved with common growth factors, demonstrating graphenes potential for stem cell research. (elsevierpure.com)
This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
Mesenchymal stem cells induced to secrete neurotrophic factors attenuate quinolinic acid toxicity: a potential therapy for Huntington's disease. (jamanetwork.com)
Sadan O, Shemesh N, Cohen Y, Melamed E, Offen D. Adult neurotrophic factor-secreting stem cells: a potential novel therapy for neurodegenerative diseases. (jamanetwork.com)

Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
They may differentiate into any form of cell in the body because they are pluripotent. (suzermedclinic.com)
Growth and Development: Stem cells divide and differentiate into specialized cell types during embryonic development, resulting in the formation of the body's many tissues and organs. (suzermedclinic.com)

Till the end of 2013 he has treated over 7,000 patients with stem cell therapy with his medical team, involving many diseases, such as cerebral palsy, spinal cord injury, traumatic brain injury, diabetes, ulcerative colitis and cirrhosis. (wikipedia.org)
My laboratory group has received funding from SFI to study the immunologic basis of acute and chronic kidney disease and how this might be altered by stem cell-based therapy. (universityofgalway.ie)
Development and optimization of the medium using CombiCult® and the close collaboration between EMD Millipore and Plasticell is described in a recent sp 2 article entitled "Applying mesenchymal stem cell technology to drug discovery and cell therapy" (sp 2 Inter-Active, January/February 2013, pp16-18, http://edition.pagesuite-professional.co.uk//launch.aspx?eid=fcbef908-228c-4cf4-9ae7-5ab1dd06ee99 ). (plasticell.co.uk)
These stem cells play an important role in several applications including cell differentiation, gene regulation, gene therapy and transplantation. (iomcworld.org)
Definitive therapy is liver transplantation, but it is constrained by difficulties in finding a transplant donor. (bsmiab.org)
Definitive therapy for cirrhosis is liver transplantation, but it takes time to find a matched donor, and thus, patients have to wait relatively for a long time and should continue to use urso deoxy cholic acid (UDCA) as an effort to slow the disease progression [ 2 , 3 ]. (bsmiab.org)
3) the possibility of a human nucleus pulposus cell line as a cell source for cell transplantation therapy. (cellsurgicalnetwork.com)
The use of olfactory progenitors as a cell-based therapy for Parkinson's disease (PD) would allow harvest without invasive surgery, provide an autologous cell population, eliminate need for immunosuppression and avoid the ethical concerns associated with embryonic tissues. (scirp.org)
stromal vascular fraction cell therapy for multiple sclerosis. (stemcellclinicsofamerica.net)
Cell-replacement therapy with stem cells in neurodegenerative diseases. (stemcellclinicsofamerica.net)

To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
1. Basic immunology with an emphasis on dendritic cell, monocyte and T cell biology. (universityofgalway.ie)

This study was developed to compare the morphological, proliferative and immunophenotypic profiles of pulp cells from permanent and primary teeth, obtained by two isolation methods. (bvsalud.org)

Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Transcription factor expression and the rate-limiting enzyme in dopamine synthesis tyrosine hydroxylase (TH) were detected in the transfected cells after 4 month-selection with G418, indicating transfected hONPs were stably restricted towards a dopaminergic lineage. (scirp.org)

Furthermore, it is unknown whether endogenous progenitors/stromal cells in the adult human heart could be chemically induced to generate functional atrial or ventricular heart muscle to treat adult heart diseases. (biomedcentral.com)
Adult stem cells: These stem cells continue to exist in the body's tissues and organs even after development. (suzermedclinic.com)
iPSCs are adult cells that have been reprogrammed to become pluripotent stem cells (or induced pluripotent stem cells, or iPSCs). (suzermedclinic.com)
Certain adult stem cell types can divide and specialize into certain cell types to replace damaged or dying cells in response to injury or everyday wear and tear. (suzermedclinic.com)

These findings suggest that factors other than neuronal replacement underlie the behavioral sparing observed in 3NP rats after MSC transplantation. (allergy-link.com)
In conclusion, addition of HuMSC-S to UDCA lowered caspase-3 levels and apoptotic cell count in rats with hepatic cholestasis after choledochal duct ligation. (bsmiab.org)
Then, the mesenchymal cells were injected to the tail vein of the healthy and irradiated (7Gy gamma ray) female rats. (ac.ir)
Following transplantation of hiPSC-MSC and hiPSC-MSC-derived chondrocytes into osteochondral defects of arthritic joints of athymic rats, magnetic resonance imaging studies showed gradual engraftment, and histological correlations demonstrated hyaline cartilage matrix production. (mdc-berlin.de)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

Anatomy21

Organisms4

Diseases13

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment47

Psychiatry and Psychology1

Phenomena and Processes24

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science1

Named Groups2

Health Care6

Stromal cell8

Progenitor cells10

MSCs33

Hematopoietic Stem4

Embryonic stem9

Endothelial8

Adipogenic3

Neural12

Fetal3

Osteogenic differentiation4

Multi-lineage differentiation4

Lineages3

Self-renewal6

Therapeutic12

Regeneration9

Precursor cells1

Organ transplantation5

Proteins2

Murine4

HMSCs4

Neurons5

Chondrogenic differentiation1

Clinical16

Tissue17

Genes8

Induce4

Induction6

Outcomes1

Immunocytochemistry4

Apoptosis5

Characterization1

Regenerative medicine2

Marrow11

Survival5

Potential7

Differentiate4

Therapy10

Biology2

Morphological1

Lineage2

Adult4

Rats5