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  • confocal
  • Flow cytometry and confocal laser scanning microscopy results showed that N-myristoylation significantly increased the membrane affinity toward breast cancer cells and also effectively mediated cellular entry. (frontiersin.org)
  • Fluorescence
  • Fluorescence, absorbance, and binding of a mitochondrial membrane potential-sensitive probe, rhodamine 800 (rhod800), were measured in isolated rat mitochondria, hepatocytes, cardiomyocytes, and hearts in the presence or absence of mitochondrial uncouplers. (spiedigitallibrary.org)
  • This resulted in a fluorescence decrease by 77% (13% in de-energized mitochondria). (spiedigitallibrary.org)
  • In hearts, binding, absorbance, and fluorescence were almost uncoupler-insensitive possibly due to rhod800 interaction outside of mitochondria. (spiedigitallibrary.org)
  • This study uses two-photon fluorescence lifetime imaging microscopy (FLIM) to investigate metabolic changes in MCF10A premalignant breast cancer cells treated with a range of glycolysis inhibitors: namely, 2 deoxy-D-glucose, oxythiamine, lonidamine, and 4-(chloromethyl) benzoyl chloride, as well as the mitochondrial membrane uncoupling agent carbonyl cyanide m-chlorophenylhydrazone. (spiedigitallibrary.org)
  • Following Cre induced recombination cells will have membrane yellow or membrane cyan fluorescence. (mbl.edu)
  • Molecular
  • We also explore the tight interplay between ER- and mitochondria-mediated Ca 2+ signalling, covering the structural and molecular properties of the zones of close contact between these two networks. (biomedcentral.com)
  • Potato
  • In this study, by means of swelling experiments in K(+) and ammonium salts, we characterize a PIMAC-like anion-conducting pathway in mitochondria from durum wheat (DWM), a monocotyledonous species phylogenetically far from potato. (semanticscholar.org)
  • experimental
  • This experimental condition results in fact in the impairment of the ER-mitochondria crosstalk and the subsequent hindering of autophagosome nucleation. (sigmaaldrich.com)
  • Importantly, experimental increase of ER-mitochondria contacts in human myotubes prevents palmitate-induced alterations of insulin signaling and action, whereas disruption of MAM integrity alters the action of the hormone. (diabetesjournals.org)
  • cells
  • Our first release was a line of products that detect apoptotic cells through the measurement of active caspases and loss of mitochondrial membrane potential. (scribd.com)
  • Accordingly, the membrane affinity toward breast cancer cells was two to threefold higher than that of normal cells. (frontiersin.org)
  • Solaini G, Sgarbi G, Lenaz G, Baracca A. Evaluating mitochondrial membrane potential in cells. (ukrbiochemjournal.org)
  • Smets I, Caplanusi A, Despa S, Molnar Z, Radu M, VandeVen M, Ameloot M, Steels P. Ca2+ uptake in mitochondria occurs via the reverse action of the Na+/Ca2+ exchanger in metabolically inhibited MDCK cells. (ukrbiochemjournal.org)
  • Following freeze-fracture of the rat epididymis, a population of cells whose apical, but not baso-lateral, plasma membrane contains elongated intramembrane particles was found in the initial segment. (biologists.org)
  • Apical cells also contain the enzyme, carbonic anhydrase which, together with the presence of rod-shaped particle on their membranes, permits their classification as 'mitochondria-rich' cells, a specialized cell type found in some transporting epithelia. (biologists.org)
  • Mitofusin 2 regulates STIM1 migration from the Ca2+ store to the plasma membrane in cells with depolarized mitochondria. (ox.ac.uk)
  • bacterial
  • That endosymbionts evolved by this process is evidenced by the fact that they have a double membrane, including their own original form that resembles the ancestral bacterial surface. (blogspot.com)
  • composition
  • We have also studied [Ca 2+ ] m changes, when incubating mitochondria in mediums of different composition: without ATP and ions of Mg (0-medium), in the presence of 3 mM Mg (Mg-medium) and 3 mM Mg + 3 mM ATP (Mg,ATP-medium). (ukrbiochemjournal.org)
  • Preincubation of mitochondria in mediums of different composition with 25 μM trifluoperazine did not affect the [Ca 2+ ] m values both before and after the addition of 100 µМ Са 2+ to the incubation medium. (ukrbiochemjournal.org)