the membrane-binding domain consists of a series of amphipathic α helices with several hydrophobic amino acids exposed to a membrane monolayer. (wikipedia.org)
As a consequence to this organelle architecture, proteins that are embedded into this monolayer membrane adopt a unique monotopic topology. (uni-saarland.de)
Amphipathic2
Binding of aa107-125 synthetic peptide with membrane-mimicking micellar surfaces induces transition from unstructured loop to amphipathic α-helical (AH) structure but this transition is automatic in the C112-palmitoylated peptide. (nih.gov)
We predict that it may also contain an overlooked, widely conserved membrane-binding amphipathic helix. (beds.ac.uk)
Lipid2
Caveolae are plasma membrane invaginations with a distinct lipid composition. (bvsalud.org)
Residues 107-125 are believed to interact with membranes but are unresolved in all RPE65 crystal structures, whereas palmitoylation at C112 also plays a role. (nih.gov)
They also suggest new models for how caveolins, major structural components of caveolae, insert into membranes and interact with lipids. (bvsalud.org)
We present new models inspired by the structure for how caveolins oligomerize, remodel membranes, interact with their binding partners, and reorganize when mutated. (bvsalud.org)
Distinct1
We will discuss how we discovered a novel targeting pathway for LD-destined proteins by combining biochemistry with state-of-the-art microscopy and quantitative mass spectrometry and furthermore, how we are currently exploring protein partitioning between membranes of distinct physico-chemical properties. (uni-saarland.de)
Sequence2
The program on the first day began with an invited keynote presentation from Dr. Michael Levitt of Stanford University in which he discussed the emerging pictures of protein structure and sequence spaces. (nih.gov)
Sequence-based prediction approaches are useful to predict a variety of structural and functional properties of proteins. (tu-muenchen.de)
Organelles1
In this lecture, we will discuss concepts on how newly synthesized proteins are targeted to their destination organelles in cells. (uni-saarland.de)
Structural2
Membrane lipids cooperate with the structural components of caveolae to generate a metastable surface domain. (bvsalud.org)
We show that P132 is positioned at a major site of protomer-protomer interactions within the CAV1 complex, providing a structural explanation for why the mutant protein fails to homo-oligomerize correctly. (bvsalud.org)
Structure5
Improvements in methodology and technology to permit the more rapid, less expensive and more predictable determination of the structure of protein targets remains a major goal of the NIGMS Protein Structure Initiative (PSI). (nih.gov)
The 2008 PSI 'Bottlenecks' meeting was held at the Natcher Conference Center on the NIH campus in Bethesda, Maryland, on April 14-16 in order to stimulate communication and dissemination of the recent progress in the constituent tasks of protein structure determination in a high-throughput setting. (nih.gov)
As in the past, topics of gene cloning, gene expression, protein purification and crystallization and rapid NMR structure determination were highlighted in the meeting program. (nih.gov)
Dr. John Norvell, director of the PSI, introduced the workshop and discussed the current progress and the future of the PSI program particularly in the context of the recent evaluation of the Protein Structure Initiative by representatives of the National Advisory General Medical Sciences Council. (nih.gov)
In general secondary structure of a protein is the 3D form constructed by inter-residue interactions like hydrogen bonds. (tu-muenchen.de)
Form1
The caveolins, a family of monotopic membrane proteins, form the inner layer of the caveolar coat. (bvsalud.org)
Domain2
Both the cyclooxygenase and the peroxidase active sites are located in the catalytic domain, which accounts for approximately 80% of the protein. (wikipedia.org)
The putative MTase-GTase domain of nodaviruses also contains membrane-binding sites that may drive the assembly of viral replication factories, revealing an unsuspected parallel with the alphavirus supergroup. (beds.ac.uk)
Association3
Although membrane association is critical for RPE65 function, its mechanism is not clear. (nih.gov)
We demonstrate that the AH significantly affects palmitoylation level, membrane association, and isomerization activity of RPE65. (nih.gov)
ssRNA viruses infecting eukaryotes replicate in the cytoplasm of infected cells in association with membranes [ 3 ] and utilize multiple strategies to express their proteins [ 4 ]. (beds.ac.uk)
Study2
These "old school" biochemical approaches are still very powerful for discovering new protein targeting pathways in cells and to study membrane protein topogenesis. (uni-saarland.de)
Sukumar, N. , Mathews, F. S. , Langan, P. , and Davidson, V. L. (2010) A joint x-ray and neutron study on amicyanin reveals the role of protein dynamics in electron transfer . (cornell.edu)
Domains1
They can be subdivided into large supergroups based on the presence of a shared set of domains in their replication proteins [ 1 , 2 ], such as the picornavirus, flavivirus, and alphavirus supergroups. (beds.ac.uk)
Multiple1
Proteins that bind PI typically have two motifs (or multiple motifs) which allow for temporal and spatial separation of binding events. (nih.gov)
Role1
Here, we summarize how these discoveries are transforming our understanding of this long-enigmatic protein family and their role in caveolae assembly and function. (bvsalud.org)