• In the effort to track the progress of an individual patient along this imaginary line, it is necessary to measure the relative contribution of the membrane and native lungs in terms of gas exchange, as well as the response of the patient's respiratory drive and mechanics to the variation in ECMO settings. (biomedcentral.com)
  • Dynamic events at the plasma membrane of live cells are commonly studied with light microscopy and single-particle tracking (SPT) after labeling of specific membrane components with individual fluorophores, colloidal gold, or fluorescent beads ( 1 - 3 ). (pnas.org)
  • Thorough analysis of the trajectories thus obtained provides information about the lateral mobility of membrane components and about the constraints imposed on them by cytoskeletal elements ( 4 - 6 ), cholesterol-rich microdomains ( 7 , 8 ), and other structures that impose heterogeneity in the plasma membrane. (pnas.org)
  • In this report we demonstrate that the human plasma membrane protein DARC can be used as an artificial anchor molecule in cell surface engineering applications. (biomedcentral.com)
  • The SNAP-tag® served as an example for a molecular-technological developed protein that is artificially attached to the extracellular side of the plasma membrane through our DARC-anchor. (biomedcentral.com)
  • In this work, we examined whether the human plasma membrane protein DARC [ 4 ] can act as such a nanobiotechnological polypeptide anchor fused with a functional peptide of interest. (biomedcentral.com)
  • Plasma membrane resealing is a Ca 2+ -dependent process that involves the exocytosis of intracellular vesicles next to the wound site. (pnas.org)
  • These findings raised the possibility that the complex pathology of lysosomal diseases might also include defects in plasma membrane repair. (pnas.org)
  • Thus, the severe symptoms exhibited by CHS patients may also include defects in the ability of cells to repair plasma membrane lesions. (pnas.org)
  • Modulation of synaptotagmin VII function through dominant-negative or gene knock-out approaches revealed a role for lysosomal exocytosis in the repair of plasma membrane lesions ( 12 , 13 ). (pnas.org)
  • Given that most cells from CHS patients and beige-J mice have enlarged lysosomes ( 2 ), these findings suggested that lysosome-mediated plasma membrane repair might also be compromised in cells carrying this mutation. (pnas.org)
  • The researchers at the University of Basel have now been able to measure this in a practical experiment for the first time, demonstrating that the thicker the membrane, the slower the movement of the channel protein is in comparison to the movement of the actual polymers that form the membrane. (nanowerk.com)
  • There are lots of membrane sugar-protein interactions that are important for disease," Percec said. (healthcanal.com)
  • Tools for the identification of trans-membrane spans from the protein sequence are widely used in the experimental community. (sciweavers.org)
  • Computational structural biology seeks to increase the prediction accuracy of such methods since they represent a first step towards membrane protein tertiary structure prediction from the amino acid sequence. (sciweavers.org)
  • We introduce a predictor that is able to identify trans-membrane spans from the sequence of a protein. (sciweavers.org)
  • Even though the binding may be predominantly to protein sites, the cooperativity may reflect changes in the electrical gradient across the mitochondrial membrane. (oregonstate.edu)
  • We demonstrate the cell-free expression of the actin-like protein MreB and observed its preference to localize and aggregate at the membrane interface of a water-in-oil-in-water droplet as happens in vivo. (gla.ac.uk)
  • Specifically, membrane-bound catechol- O -methyltransferase (MBCOMT) is an integral membrane protein that catalyzes the methylation of catechol substrates and has been linked to several diseases such as Parkinson's disease and Schizophrenia. (biomedcentral.com)
  • Our results demonstrate that artificial channels can be designed for protein transport via block copolymer self-assembly using classical methods of membrane preparation. (edu.sa)
  • The silent Duffy antigen/receptor for chemokines (DARC) is a receptor-like membrane protein of erythrocytes and mediates no cell transduction not at least regarding a missing or truncated G-loop and therefore it seemed to be the candidate for our cell membrane anchor. (biomedcentral.com)
  • A proton-pumping protein uses this stored energy to generate proton motive force across the membrane, which in turn drives the synthesis of adenosine triphosphate (ATP). (elsevier.com)
  • The development of synthetic biomimetic artificial water-channels and pores is key for a better understanding of the natural function of protein channels. (rsc.org)
  • While synthetic chemists have produced sophisticated architectures able to confine water clusters, most water channel based work is being conducted with natural protein channels as selectivity components, embedded in the diverse arrays of bio-assisted artificial systems. (rsc.org)
  • Most protein channels share some structural aspects, such as their self-assembled multiple subunits within trans-membrane domains and the selectivity of these protein channels is usually driven by the narrowest region of the pore, showing gating behaviours generated by the structural motion of the external subunits in response to voltage, ligand and pH stimuli. (rsc.org)
  • Immunofluorescence with mAbs against rat (LY1C6, provided by I. Mellman, Yale University, New Haven, CT), human (H4A3), or mouse (Protein Data Bank ID code 1D4B) lysosomal-associated membrane protein 1 (Lamp1) (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City) was performed as described for attached cells ( 13 ). (pnas.org)
  • The addition of a hydrophobic group to a protein forms a lipid anchor that attaches the protein to the lipid membrane. (medgadget.com)
  • By using a sensitive electroporation/fluorescence-activated cell sorter-based assay, we show that lysosomal exocytosis triggered by membrane wounding is impaired in both human Chediak-Higashi and mouse beige-J fibroblasts. (pnas.org)
  • Rapid purification of functional cytochrome P-450 and P-450 reductase by high pressure liquid chromatography utilizing immobilized artificial membranes (IAM): A new solid phase membrane mimetic matrix. (springer.com)
  • By means of lipid dip-pen nanolithography (L-DPN), lipid membranes are written directly onto the two-dimensional carbon graphene. (kit.edu)
  • Researchers around Dr. Michael Hirtz from Karlsruhe Institute of Technology and Dr. Aravind Vijayaraghavan from the University of Manchester have developed a new method to produce artificial membranes: Using a nanoscaled tip, they write tailored patches of phospholipid membrane onto a graphene substrate. (kit.edu)
  • M. Hirtz, A. Oikonomou, T. Georgiou, H. Fuchs, & A. Vijayaraghavan: Multiplexed biomimetic lipid membranes on graphene by dip-pen nanolithography. (kit.edu)
  • Researchers at the U.K.'s University of Manchester and Germany's Karlsruhe Institute of Technology have developed a method by which they can create highly detailed cell membrane models on a graphene surface, providing a way to study how drugs behave when delivered across the lipid bilayer. (fiercepharma.com)
  • The lateral mobility of individual murine polyoma virus-like particles (VLPs) bound to live cells and artificial lipid bilayers was studied by single fluorescent particle tracking using total internal reflection fluorescence microscopy. (pnas.org)
  • Membrane microfiltration helps remove unwanted particles from water before the water is treated for purity using other processes such as reverse osmosis. (prnewswire.co.uk)
  • By employing theoretical models and performing tests on cultured cells, the researchers found that it took less energy for a cell membrane to engulf disc-shaped particles than rod-shaped ones, which they had originally expected to be the most efficient. (fiercepharma.com)
  • The membrane coating is formed from cordierite particles which have been processed to have a median particle size diameter of between 1 and 3 microns with a narrow particle size distribution suitable for forming a cordierite membrane on a cordierite monolith substrate. (freepatentsonline.com)
  • Here, trajectories of individual, fluorescence-labeled VLPs on the surface of tissue culture cells and in artificial lipid bilayers were recorded with total internal reflection fluorescence (TIRF) microscopy for SPT. (pnas.org)
  • However, it is very difficult to study the membranes directly in live cells inside the human body. (kit.edu)
  • Cells use lipid remodeling to respond to their environment and maintain membrane homeostasis or to carry out specific functions such as division and signaling," said a graduate student in the Devaraj lab, Andrew Rudd, one author of the study. (labroots.com)
  • Microbial fuel cells and osmotic membrane bioreactors have mutual benefits for wastewater treatment and energy production. (ucdenver.edu)
  • Currently desired manipulations of eukaryotic cells comprise the specific modification of their extracellular surface, in particular the cell membrane. (biomedcentral.com)
  • Fortunately, there is much to be learned from tackling a more modest goal, that of building simple artificial cells, beginning with their walls. (newscientist.com)
  • If we can find it in artificial cells, we will be transported back to the onset of Darwinian evolution and the origins of life as we know it. (newscientist.com)
  • We are looking for Honours or PhD students who have an interest in DNA origami design, synthetic biology, and artificial cells to learn across the interface of engineering and biology. (edu.au)
  • In addition, the survival of Chediak-Higashi and beige-J fibroblasts after wounding was reduced, indicating that impaired lysosomal exocytosis inhibits membrane resealing in these mutant cells. (pnas.org)
  • A Traube cell is an "artificial cell" created by Moritz Traube in order to study the processes of living cells, including growth and osmosis. (wikipedia.org)
  • The artificial cell would expand and bud like living cells. (wikipedia.org)
  • The ability of the Traube cell membrane to allow water to flow in while retaining the cell solute is comparable to living cells. (wikipedia.org)
  • The chair of Bioartificial Organs (BAO) at the Department of the Biomaterials science and Technology (BST), Faculty of Science and Technology (TNW) of the University of Twente has an opening for a post-doctoral position for 2 years on the development of new membrane concepts for portable/ wearable artificial kidney devices. (twente.com)
  • To demonstrate the utility of their new model, the researchers studied how mutant varieties of Gal-8 interacted with a custom artificial membrane containing Gal-8's specific binding sugars. (healthcanal.com)
  • Consequently, researchers frequently use model membranes that are applied to special surfaces. (kit.edu)
  • Researchers at the University of California San Diego have been hard at work developing artificial replicas of cell membranes. (labroots.com)
  • Researchers in Japan have engineered a membrane with advanced features capable of removing harmful greenhouse gases from the atmosphere. (ecnmag.com)
  • Easan Sivaniah - an associate professor at Kyoto University's Institute for Integrated Cell-Material Sciences (iCeMS) - led an international team of researchers from iCeMS and the University of Cambridge to create an advanced membrane capable of rapidly separating gases. (ecnmag.com)
  • Like a pen on paper, the researchers say, the sharp tip allows for the creation of artificial membranes with nano-scale precision. (fiercepharma.com)
  • The L-DPN technique uses a very sharp tip to write lipid membranes onto surfaces in a way similar to what a quill pen does with ink on paper," explains Dr. Michael Hirtz from the INT. This tip has an apex in the range of a few nanometers only and is controlled with a high precision by a machine. (kit.edu)
  • Transmission electron microscopy of the membrane cross-sections confirmed the formation of channels with hydroxyl groups beneficial for hydrogen-bond forming sites. (edu.sa)
  • Artificial reaction centers can be prepared from porphyrins and other chromophores, electron donors, and electron acceptors linked by covalent bonds. (elsevier.com)
  • Design and synthesis of artificial neural networks capable of directional electron migration. (ncl.ac.uk)
  • In a move that brings mankind another step closer to being able to create artificial life forms from scratch, chemists from the University of California, San Diego (UCSD), and Harvard University have created artificial self-assembling cell membranes using a novel chemical reaction. (slashdot.org)
  • The novelty of the approach presented here is the simultaneous prediction of trans-membrane spanning -helices and -strands within a single tool. (sciweavers.org)
  • Therefore, the main aim of this work was to optimize the temperature, dimethylsulfoxide (DMSO) concentration and the methanol flow-rate for the biosynthesis of recombinant MBCOMT by Pichia pastoris bioreactor methanol-induced cultures using artificial neural networks (ANN). (biomedcentral.com)
  • For this, membrane glycans are first metabolically labeled with azido sugars and then tagged with biotin by copper-free click chemistry. (uni-muenchen.de)
  • Primitive membranes made from fatty acids seem to have all the right properties, such as allowing for spontaneous growth and division, as well as letting nutrients penetrate the cell. (newscientist.com)
  • These implants use silicon nanofluidic membranes, each of which has a precise number of identical nanochannels. (phys.org)
  • This leading-edge membrane technology studied at Houston Methodist presents key properties for use in a drug-delivery implant-mechanical robustness, biochemical inertness and high-density of nanochannels that allow drug delivery at clinical doses from a tiny membrane. (phys.org)
  • THOMAS, D. and CAPLAN, S.R., in "Membrane Separation Processes", Ed. Meares P. Elsevier Publishing Co. Amsterdam (1974). (springer.com)
  • The need to functionalize cell membranes in a directed way for specific applications as single cell arrays or to force close cell-to-cell contact for artificial intercellular interaction and/or induction concerning stem cell manipulation or in general to have a tool for membrane and cell surface-associated processes, we envisaged a neutral inactive membrane anchor for extracellular entities to facillitate the above mentioned functionalities. (biomedcentral.com)
  • Membrane microfiltration is also increasingly used in biodiesel processes where this technology is used to reuse water especially in areas of water scarcity. (prnewswire.co.uk)
  • For decades, these challenges hindered scientific efforts to create artificial membranes for research. (healthcanal.com)
  • Artificial Lipid Membrane Report by Material, Application, and Geography - Global Forecast to 2022 is a professional and in-depth research report on the world's major regional market conditions, focusing on the main regions (North America, Europe and Asia-Pacific) and the main countries (United States, Germany, united Kingdom, Japan, South Korea and China). (reportsmonitor.com)
  • A key challenge in membrane receptor research is to maintain natural environment. (biomimeticsgroup.com)
  • Adoption of membrane microfiltration in milk is one of the trends being witnessed in the global market, according to a new research study on the global membrane microfiltration market by Future Market Insights. (prnewswire.co.uk)
  • This nanochannel delivery system (nDS), designed by Grattoni and Mauro Ferrari, Ph.D., president and CEO of the Houston Methodist Research Institute, and colleagues, is a membrane that acts as a filter with hundreds of thousands of uniform nanoscale channels. (phys.org)
  • To test these differences, the research team took their membranes and developed them with different channel sizes, going in incremental steps from very small channels at the ultra-nanoscale all the way up to almost the micron scale, ranging from 2.5 to 250 nanometers wide. (phys.org)