Membrane protein transmembrane. Medical search. Frequent questions

It is proven that small molecule drugs can modulate these transmembrane receptors. (synabs.be)
However, most proteins in the human proteome lack small-molecule probes, including the large class of non-catalytic transmembrane receptors, such as death receptors. (rcsb.org)
Over the next five years, our goals are to (1) identify novel recycling receptors required at different Golgi compartments and establish a systematic map of the intra-Golgi recycling network, (2) determine how the transmembrane receptors engage with their cargos, and (3) define the novel functions of a disease-associated membrane transporter in solute transport and protein recycling in the Golgi. (cornell.edu)
G-protein coupled receptors (GPCRs) are membrane proteins that transmit the effects of extracellular ligands to effect changes in the intracellular G-protein signaling system. (gauss-centre.eu)
This process depends on the general physico-chemical features of the cargo membrane protein and on the interactions of these features with the collective properties of the bilayer, instead of the one-to-one intermolecular interactions that exist between discrete signals and their receptors. (biologists.com)
CD39 is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). (biolegend.com)
The valuable source of large-scale genomic information initiated attempts to identify the origin(s) of G protein-coupled receptors (GPCR), count and categorize those genes, and follow their evolutionary history. (springer.com)
Schioth HB, Fredriksson R (2005) The GRAFS classification system of G-protein coupled receptors in comparative perspective. (springer.com)
Sequence comparison does not support an evolutionary link between halobacterial retinal proteins including bacteriorhodopsin and eukaryotic G-protein-coupled receptors. (springer.com)
Violin JD, Lefkowitz RJ (2007) Beta-arrestin-biased ligands at seven-transmembrane receptors. (springer.com)
The lectures address the three different main types of membrane proteins and associated cellular processes: transport and transporters, signal transduction and receptors, bioenergetics and photosynthetic and respiratory proteins. (lu.se)
The nicotinic receptors are cylindrically-shaped proteins imbedded in synaptic walls that act as chemically-controlled sodium channels (also called ligand-gated sodium channels) that penetrate through the cell walls of post-synaptic nerves and myocytes at the skeletal neuromuscular junctions. (cdc.gov)

The endoplasmic reticulum (ER) membrane protein complex (EMC) has been shown to be a key factor in the biosynthesis and stable expression of multi-pass transmembrane proteins, and its loss is thought to cause retinal degeneration. (bioquicknews.com)
The team has previously shown that the protein Rab1 is involved in the transport of materials from the endoplasmic reticulum to Golgi, that glycosylphoshatidylinositol (GPI) synthesis is necessary for rhodopsin sorting in the trans-Golgi network, and that the Rab11/dRip11/Myo V complex is essential for post-Golgi transport of rhodopsin. (bioquicknews.com)
Secretory proteins exit the endoplasmic reticulum (ER) in coat protein complex II (COPII)-coated vesicles and then progress through the Golgi complex before delivery to their final destination. (biologists.com)
I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)

Treatment is supportive through aggressive multidisciplinary care along with small-molecule correctors and potentiators targeting the cystic fibrosis transmembrane conductance regulator protein defect. (msdmanuals.com)
It encodes a membrane-associated protein called the cystic fibrosis transmembrane conductance regulator (CFTR). (msdmanuals.com)
Cystic fibrosis, a genetic disorder defined by variants in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, affects more than 30?000 individuals in the US and approximately 89?000 worldwide. (cdc.gov)

Advances in Molecular Understanding of α-helical Membrane-Active Peptides. (muni.cz)
Enhanced translocation of amphiphilic peptides across membranes by transmembrane proteins. (muni.cz)

The CFTR gene provides instructions for making a protein called the CF transmembrane conductance regulator (CFTR). (medlineplus.gov)
The CFTR protein also regulates the function of other channels, such as those that transport positively charged particles called sodium ions across cell membranes. (medlineplus.gov)
These mutations allow the CFTR protein to retain some of its function. (medlineplus.gov)
Most of these mutations change single protein building blocks (amino acids) in the CFTR protein or delete a small amount of DNA from the CFTR gene. (medlineplus.gov)
The most common mutation, called delta F508, is a deletion of one amino acid at position 508 in the CFTR protein. (medlineplus.gov)
The CF gene encodes the CF transmembrane regulator (CFTR), a membrane bound protein capable of chloride ion transport [ 1 ]. (hindawi.com)
CFTR is a cyclic adenosine monophosphate (cAMP)-regulated chloride channel, regulating chloride, sodium, and bicarbonate transport across epithelial membranes. (msdmanuals.com)
The CFTR variants have been divided into 6 classes based on how the variant affects the function or processing of the CFTR protein. (msdmanuals.com)
Absent or decreased function of the CFTR protein is associated with multiorgan dysfunction and shortened life expectancy. (cdc.gov)
CFTR is an anion channel in the apical membrane of epithelial cells. (cdc.gov)

It mediates fusion of synaptic vesicles with the presynaptic plasma membrane resulting in exocytosis of neurotransmitters. (researchgate.net)
Lipid signaling employs various messenger molecules that carry out communication between the plasma membrane, the endomembrane compartments, and the cytoplasm. (frontiersin.org)
세포막(cell membrane) 또는 원형질막(plasma membrane)은 끊임없이 변화하는 풍경과 같습니다. (jove.com)
The mobility of PDR5-GFP has been studied using confocal FRAP in supported membranes (tethered and untethered) and compared with that of the protein in the yeast plasma membrane. (aiche.org)

The resulting abnormal channel breaks down shortly after it is made, so it never reaches the cell membrane to transport chloride ions. (medlineplus.gov)

The "wheel-like" pattern of resonances in the spectrum is characteristic of a slightly tilted membrane-spanning helix. (rcsb.org)
The analysis of the PISA wheels and Dipolar Waves obtained from both weakly and completely aligned samples show that Vpu(2-30+) has a trans-membrane alpha-helix spanning residues 8-25 with an average tilt of 13 degrees. (rcsb.org)
The backbone dynamics of the amyloid precursor protein (APP) transmembrane helix was compared to those of other transmembrane domains. (cipsm.de)

In previous work, we found that gain-of-function mutations that hyperactivate GEM-1 (an SLC16A transporter protein) can bypass the requirement for GON-2 (a TRPM channel protein) during the initiation of gonadogenesis in C. elegans. (cipsm.de)
For example, Streptomyces griseus encodes in the following order: an ABC-2 type transporter, an ABC-type ATPase (see TC#3.A.1.105.11), a S2P-M50 peptidase with a CBS domain (see 9.B.149.1.1), a lantibiotic synthesis protein and a lantibiotic dehydratase. (tcdb.org)
Peptidase processing of bacteriocins is known in some cases to occur during transport, and the peptidase and transporter presumably form a complex in the membrane. (tcdb.org)

These particular pathologies related to membrane receptor dysfunction have two distinct forms. (synabs.be)
Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. (hmdb.ca)
Kim JY, Haastert PV, Devreotes PN (1996) Social senses: G-protein-coupled receptor signaling pathways in Dictyostelium discoideum . (springer.com)
Nordstrom KJ, Sallman Almen M, Edstam M et al (2011) Independent HHsearch, Needleman-Wunsch-based, and motif analyses reveal the overall hierarchy for most of the G protein-coupled receptor families. (springer.com)

CRISPRi genetic interaction analysis revealed Retro-2 activity resembles disruption of the transmembrane domain recognition complex (TRC) pathway, which mediates post-translational ER-targeting and insertion of tail-anchored (TA) proteins, including SNAREs required for retrograde transport. (elifesciences.org)
Bsd2 binds the ubiquitin ligase Rsp5 and mediates the ubiquitination of transmembrane proteins. (wikigenes.org)

The trans-membrane protein - proteorhodopsin (pR) has been incorporated into supported lipid bilayers (SLB). (whiterose.ac.uk)
Central to our approach is to build templated solid supported lipid bilayers that utilize bacteriorhodopsin (bR), a transmembrane protein, as a tethering molecule. (aiche.org)
Native membrane derived polymer-supported lipid bilayers (nSLBs) are poised to bridge the gap between live cell experiments and traditional model membrane architectures that by offering a combination of accessibility by surface sensitive analytical instrumentation and a composition which more closely resembles cellular membranes by displaying a diversity of endogenous membrane proteins, lipids, and carbohydrates. (lu.se)
We recently developed a generic method for producing polymer-supported lipid bilayers directly from cell-derived native membrane vesicles (NMVs). (lu.se)

[ 1 ] This protein, also known as polymorphic epithelial mucin or epithelial membrane antigen, has a large extracellular region, a transmembrane sequence, and a cytosolic domain. (medscape.com)

article{Oswald2014, abstract = {Analysis of single-membrane-protein trajectories obtained using single-molecule fluorescence microscopy in living bacteria provides profound insight into protein diffusive motion. (dissem.in)

The aim of the proposed challenging project is to develop a new method, using which we will elucidate the relationship between protein sequence and preferred composition and curvature of human membranes. (muni.cz)
Determination of the transmembrane topology of a protein starts with a model of the protein based on sequence information and theoretical methods. (lu.se)
We are also interested in understanding how oligomerization specificity is encoded in protein sequence and structure, in particular in coiled-coils. (lu.se)

New technologies for purifying membrane-bound protein complexes in combination with cryo-electron microscopy (EM) have recently allowed the exploration of such complexes under near-native conditions. (researchgate.net)

I further investigated membrane proteins and the targeting of tail-anchored proteins at Sheffield Hallam University. (brookes.ac.uk)

Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
The assembly of integral membrane protein complexes is frequently supported by transmembrane domain (TMD) interactions. (cipsm.de)
Here, we present the BLaTM assay that measures homotypic as well as heterotypic TMD-TMD interactions in a bacterial membrane. (cipsm.de)
The combination of such self-organizational phenomena with canonical intermolecular interactions is most likely to control the release of membrane proteins from the ER into the secretory pathway. (biologists.com)
I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
Physiologically, the MUC1 protein may be involved in protection, lubrication, and cell adhesion by decreasing the degree of cell to extra-cellular matrix and cell to cell interactions. (medscape.com)
Sanowar S, Singh P, Pfuetzner RA, Andre I, Zheng H, Spreter T, Strynadka NC, Baker D, Goodlett DR and Miller S. Interactions of the Transmembrane Polymeric Rings of the Salmonella enterica Serovar Typhimurium Type III Secretion System. (lu.se)

Ions channels are specific transmembrane proteins - encoded by more than 500 genes in human body - whose role is the selective passage of ions across the cell membrane following electrochemical gradients. (synabs.be)
HomB protein contributes to the colonization and persistence of H. pylori , and the presence of homB genes affects the number of bacteria adhering to the host cells. (nature.com)
Mice have their own set of genes that code for the proteins that will make and sustain a mouse, much like we have our own genes that make us human. (mcgill.ca)
Familial AD (FAD) cases are caused by autosomal dominant mutations in the genes for amyloid precursor protein (APP) and the presenilins (PS1 and PS2) ( Sisodia and St George-Hyslop, 2002 ). (jneurosci.org)
This study aimed to evaluate the expression levels of miR-34a and 11 of its bioinformatically selected target genes and proteins to test their potential dysregulation in RCC. (hindawi.com)

In this review we will highlight mechanisms that control targeting of PSD-95 at the synapse, and discuss how this molecule influences the retention and clustering of diverse synaptic proteins to regulate synaptic structure and strength. (frontiersin.org)

Native and redesigned proteins exhibited highly similar characteristics, predicted structures and binding pockets, and slightly different docking poses against known ligands, although task-specific designs are still required for proteins more susceptible to internal hydrogen bond formations. (mit.edu)

Firm attachment of TP to biological membrane is aided by a special class of membrane lipids, called annular lipid shell . (wn.com)

H. pylori attaches and colonizes to the human epithelium using some of their outer membrane proteins (OMPs). (nature.com)
Outer membrane proteins (OMPs) of H. pylori play a crucial role in the host-pathogen interaction, virulence and pathogenesis. (nature.com)
Hom (Helicobacter outer membrane) family of OMPs in H. pylori consists of four members (HomA, B, C and D). In the H. pylori genome jhp 0870 open-reading frame (ORF) that codes for HomB outer membrane protein is associated with many stomach diseases and is a novel co-marker for peptic ulcer disease (PUD) 9 , 10 . (nature.com)
Outer membrane proteins (OMPs) are distinctive features of Gram-negative bacteria. (nature.com)
The coat on the budding vesicle comprises two layers, an inner layer of adaptor proteins (gray ovals) and an outer layer that forms a polyhedral cage. (biologists.com)

PRR is a single trans-membrane protein that binds prorenin and renin with comparable affinity, facilitating local AngII generation. (mtu.edu)

The structural features of the trans-membrane domain are determinants of the ion-channel activity that may be associated with the protein's role in facilitating the budding of new virus particles from infected cells. (rcsb.org)
Arkin, IT 2002, ' Structural aspects of oligomerization taking place between the transmembrane α-helices of bitopic membrane proteins ', Biochimica et Biophysica Acta - Biomembranes , vol. 1565, no. 2, pp. 347-363. (huji.ac.il)
The recombinant membrane proteins could be used in high throughput screening, in structural biology of membrane proteins crucial to structure-based drug design endeavors, and in structural genomics efforts of membrane proteins. (sbir.gov)
In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)
Our study provides essential structural information of unexplored proteins of the Hom family that can help in a better understanding of H. pylori pathogenesis. (nature.com)
SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
Our goal is to use high-resolution structural modeling techniques guided by constraints taken from lower resolution experimental data to generate structural models of important biological protein assemblies for which high resolution structural determination is unfeasible. (lu.se)

Golgi resident proteins, such as glycosyltransferases and sugar nucleotide transporters, are precisely distributed across the Golgi stacks by recycling mechanisms that counteract the flow of ongoing vesicular transport. (cornell.edu)

Five examples with varying transmembrane helices in different categories were used to demonstrate the feasibility. (mit.edu)

Nearly all of the resonances in the two-dimensional 1H/15N HSQC spectrum of uniformly 15N labeled Vpu(2-30+) in micelles are superimposable on those from the corresponding residues in the spectrum of full-length Vpu, which indicates that the structure of the trans-membrane domain is not strongly affected by the presence of the cytoplasmic domain at its C terminus. (rcsb.org)
Macroautophagy/autophagy involves the formation of an autophagosome, a double-membrane vesicle that delivers sequestered cytoplasmic cargo to lysosomes for degradation and recycling. (nih.gov)
The objective is to yield supported membranes with a bilayer to substrate spacing that can accommodate membrane proteins with large cytoplasmic domains. (aiche.org)

Analysis of an 11.7 kb DNA fragment of chromosome XI reveals a new tRNA gene and four new open reading frames including a leucine zipper protein and a homologue to the yeast mitochondrial regulator ABF2. (wikigenes.org)

The ALS8 protein VAPB interacts with the ER-Golgi recycling protein YIF1A and regulates membrane delivery into dendrites. (nih.gov)
A novel Golgi membrane protein is part of a GTPase-binding protein complex involved in vesicle targeting. (nih.gov)
however, the mechanistic basis of how most Golgi proteins are selected for recycling, or how these processes are regulated are poorly understood. (cornell.edu)
In preliminary results, we have identified novel transmembrane components orchestrating recycling of specific subsets of Golgi enzymes. (cornell.edu)

78573S The majority of membrane proteins are very difficult to obtain in significant quantities, even at milligram scale, because their natural biosynthesis levels often are very low and currently available protein expression systems are not effective for membrane proteins. (sbir.gov)
My additional independent research on ER localisation and splicing in auxin biosynthesis showed for the first time ER-localisation for an auxin biosynthetic protein. (brookes.ac.uk)

Lin F, Morrison JM, Wu W, Worman HJ: MAN1, an integral protein of the inner nuclear membrane, binds Smad2 and Smad3 and antagonizes transforming growth factor-beta signaling. (hmdb.ca)

Due to the lack of detergent solubilization and reconstitution steps, the nSLBs created using this approach contain essentially all of the native lipids, as well as the membrane-associated proteins and carbohydrates from the donor membrane. (lu.se)

In particular, we are interested in a subclass of protein assemblies, homomeric assemblies, which are produced by the repetition of single type of subunit. (lu.se)

The lack of effective method for preparative-scale membrane protein synthesis hampers progress toward a complete understanding of the proteome and prevents the exploitation of available sequences, especially those membrane proteins which constitute the majority of drug targets. (sbir.gov)
2004). RseP (YaeL), an Escherichia coli RIP protease, cleaves transmembrane sequences. (tcdb.org)

2020). Functional Characterization of TMEM127 Variants Reveals Novel Insights into Its Membrane Topology and Trafficking. (tcdb.org)
The main aim of the course is to enable students to acquire specialised knowledge and understanding of membrane biochemistry and the molecular structure, topology and functional mechanisms of membrane proteins. (lu.se)

The three-dimensional structure of the channel-forming trans-membrane domain of virus protein "u" (Vpu) of HIV-1 was determined by NMR spectroscopy in micelle and bilayer samples. (rcsb.org)
Transmembrane immunoglobulin and munin domain (TIM) proteins are a family of cell surface immunomodulatory proteins. (wikipedia.org)
This domain has not been found in proteins other than TMEM53 and its orthologs. (wn.com)
For membrane proteins, a third mechanism, based on the interaction of their transmembrane domain (TMD) with lipid microdomains, must also be considered. (biologists.com)
The γ-subunits of the so-called AGG3 type, which comprise a transmembrane domain, are exclusively found in plants. (lu.se)

CrMP-Sol also presents QTY-enabled water-soluble designs to facilitate native membrane protein studies despite natural hydrophobicity. (mit.edu)
The methodology presented allows the amount of native membrane material in the nSLB to be precisely controlled and display a uniform lateral distribution. (lu.se)

Zinc ions have shown antiviral properties, but a key issue for their use for antiviral therapy is its difficulty, as a divalent metal ion, to cross the cell membrane and thus reach its targets inside the cell. (gauss-centre.eu)
Depolarization is followed by the opening of other transmembrane channels that selectively allow the flow of K+ ions into the cell. (cdc.gov)

Transmembrane protein 53 , or TMEM53 , is a protein that is encoded on chromosome 1 in humans. (wn.com)

We find that despite its broad metal-recognition profile, MntBC-A imports only manganese, whereas zinc can function as a high-affinity inhibitor of MntBC-A. Computational analysis shows that the transmembrane metal permeation pathway is lined with six titratable residues that can coordinate the positively charged metal, and mutagenesis studies show that they are essential for manganese transport. (nih.gov)

In this Commentary, I review evidence in favor of the idea that partitioning of TMDs into bilayer domains that are endowed with distinct physico-chemical properties plays a pivotal role in the transport of membrane proteins within the early secretory pathway. (biologists.com)

Many TPs function as gateways or "loading docks" to deny or permit the transport of specific substances across the biological membrane, to get into the cell, or out of the cell as in the case of waste byproducts. (wn.com)
This protein has been implicated has having a role in T cell function and development. (wn.com)
Finally, through a common trick known as alternative splicing, the RNA transcribed from the klotho gene can end up making a much shorter protein that can't grow out of the cell like a flower but that is free to circulate in the blood. (mcgill.ca)
Thus, suppressing PA production by inhibiting either PLD or DGK activity compromised membrane trafficking except early endocytosis, disrupted tip-localized deposition of cell wall material, especially pectins, and inhibited pollen tube growth. (frontiersin.org)
Cell-based and in vitro assays show that Retro-2 blocks delivery of newly-synthesized TA-proteins to the ER-targeting factor ASNA1 (TRC40). (elifesciences.org)
and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
Together, overexpressed POLRMT is an important mitochondrial protein for prostate cancer cell growth, representing a novel and promising diagnostic and therapeutic oncotarget. (bvsalud.org)
As cell membranes are both dynamic and compositionally complex, replicating these aspects in a model membrane are essential. (lu.se)
Cancer antigen 27-29 (or CA 27-29) is an epitope of a large transmembrane mucin glycoprotein named MUC1 that is expressed on the cell surfaces of most glandular epithelia. (medscape.com)
This macromolecular assembly comprises more than 20 conserved proteins that form a series of ring-like structures and a needle-shaped protrusion, which allows the transport of proteins into a host cell. (lu.se)

Selecting Collective Variables and Free Energy Methods for Peptide Translocation Across Membranes. (muni.cz)

Heterotrimeric G proteins are intracellular membrane-attached signal transducers involved in various cellular processes in both plants and animals. (lu.se)

Oesterhelt D (1998) The structure and mechanism of the family of retinal proteins from halophilic archaea. (springer.com)

A transmembrane protein (TP) is a type of membrane protein spanning the entirety of the biological membrane to which it is permanently attached . (wn.com)
As a response to the shape of certain molecules these "freight handling" TPs may have special ways of folding up or bending that will move a substance through the biological membrane. (wn.com)
Recent years have seen strong research efforts on the lipid component of biological membranes. (lu.se)
In my talk, I will show you some examples how such biological cues influence the dynamic properties of membranes. (lu.se)
Many biological processes are controlled by large multi-component protein assemblies, the size and complexity of which typically precludes the determination of their high-resolution structures. (lu.se)

Crystal structures often deviate from the natural system: Proteins, especially membrane-bound ones, do not necessarily crystallize in their biologically active structures and the measures needed to obtain suitable GPCR crystals tend to increase the diversity between the natural environment and the crystal. (gauss-centre.eu)
The β-secretase cuts APP first to generate the N terminus of Aβ, thus producing a membrane bound C-terminal fragment called C99. (jneurosci.org)
While many studies have been focused on the membrane structure, the dynamics of such systems are crucial for the function of the membrane including membrane bound proteins. (lu.se)

Hereditary malfunctions of the transmembrane protein result from genetic mutations in the subunits of an ion channel or in the regulatory proteins linked to it. (synabs.be)
Mounting evidence suggests that the genetic disorders/mutation and diseases change not only the protein expression patterns but also membranes themselves. (lu.se)
This is followed by experimental determination using genetic construction and expression of a fusion protein of the membrane protein and a marker protein in a bacterial system which is subsequently analysed. (lu.se)

Together, our work explains how Retro-2 prevents retrograde trafficking of toxins by inhibiting TA-protein targeting, describes a general CRISPR strategy for predicting the MOA of small molecules, and paves the way for drugging the TRC pathway to treat broad classes of viruses known to be inhibited by Retro-2. (elifesciences.org)

Pan D, Estevez-Salmeron LD, Stroschein SL, Zhu X, He J, Zhou S, Luo K: The integral inner nuclear membrane protein MAN1 physically interacts with the R-Smad proteins to repress signaling by the transforming growth factor-{beta} superfamily of cytokines. (hmdb.ca)
Spatially regulated ubiquitin ligation by an ER/nuclear membrane ligase. (wikigenes.org)
Previous work in this area has been on modeling the assembly structure of the type III secretion system and prediction of the high-resolution structures of homo-oligomeric proteins using limited experimental data from Nuclear Magnetic Resonance (NMR) in order to speed up the process of structure determination. (lu.se)

The groups used very different isolation methods (i.e., expression cloning, protein purification, genomics), yet all identified the same enzyme and concurred that it possessed all the known characteristics of β-secretase ( Fig. 1 ) ( Cole and Vassar, 2008 ). (jneurosci.org)
Lectures dealing with methods for theoretical modelling of membrane protein structure, fusion protein techniques, X-ray crystallography, heterologous expression, solubilisation and purification of membrane proteins are also included in the course. (lu.se)
In silico exercise addressing potential problems concerning the detection of heterologously expressed membrane proteins, solubilisation and evaluation of detergent properties, ion exchange chromatography and gel filtering in the presence of a detergent, and control of the protein's stability and integrity after purification. (lu.se)

Bacteriorhodopsin tether supported lipid bilayer membranes were constructed onto these streptavidin-functionalized silica particles (5 micron) using a detergent-based reconstitution method in which the tethered bR conjugates template the assembly of the supported bilayer. (aiche.org)

Magainin 2 and PGLa in Bacterial Membrane Mimics III: Membrane Fusion and Disruption. (muni.cz)

The Gamblin laboratory studies the mechanisms that lead to the polymerization of the microtubule-associated protein tau. (utsa.edu)
Next, western blotting was utilized to examine the protein expressions of microtubule-associated protein 1 light chain 3 (LC3), p62, hypoxia-inducible factor 1-alpha (HIF-1α) and Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 (BNIP3). (bvsalud.org)

Recent advances in biophysical methods have been able to shed more light on the structures of helical bundles formed by the transmembrane segments of bitopic membrane proteins. (huji.ac.il)
Finally, the structures of the best characterized instances of transmembrane α-helical bundles formed by bitopic membrane proteins are described in detail. (huji.ac.il)

Recently, Dr. Satoh's group performed the genome-wide screening of Drosophila mutants, and identified 233 mutants that failed to synthesize and/or transport rhodopsin to the photosensitive membrane of the rhabdomeres. (bioquicknews.com)

Understanding the mechanisms underlying protein folding and trafficking may contribute to the large-scale, therapy-based production of target proteins. (bioquicknews.com)
Although gene profiling can identify their cancer-specific signatures, systematic correlations between protein functions and tumor-related mechanisms are still unclear. (mit.edu)
A number of proteins from each process, for which the structure is known, are explored in greater detail in order to highlight the functional molecular mechanisms. (lu.se)

A research team led by Prof. Holger Gohlke at the Heinrich Heine University Düsseldorf is using supercomputing resources at the Jülich Supercomputing Centre (JSC) to better understand so-called hyperpolarization-activated cyclic nucleotide-gated (HCN) channels, which serve as crucial ion channels in the membrane for controlling electric pulses in the brain and heart, among other fundamental processes in the body. (gauss-centre.eu)

Anatomy40

Organisms10

Diseases1

Chemicals and Drugs103

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes67

Disciplines and Occupations2

Technology, Industry, Agriculture8

Information Science4

Health Care1

Receptors12

Endoplasmic reticulum4

Cystic fibrosis3

Peptides2

CFTR10

Plasma membrane4

Chloride1

Helix3

Transporter3

Receptor4

Mediates2

Lipid bilayers4

Epithelial1

Abstract1

Sequence3

Purifying membrane-bound1

Tail-anchored proteins1

Interactions8

Genes5

Synaptic1

Ligands1

Class of membrane1

Outer5

Trans-membrane protein1

Structural7

Transporters1

Helices1

Cytoplasmic3

Regulator1

Golgi4

Biosynthesis2

Binds1

Lipids1

Subunit1

Sequences2

Topology2

Domain5

Native membrane2

Ions2

Chromosome1

Residues1

TMDs1

Cell10

Translocation1

Intracellular1

Mechanism1

Biological5

Bound3

Genetic3

Pathway1

Nuclear3

Purification3

Bacteriorhodopsin1

Disruption1

Microtubule-associa2

Bundles2

Transport1

Mechanisms3

Crucial1