• We identified 231 proteins released from actomyosin contraction-dependent blebs and apoptotic bodies by adapted SILAC (stable isotope labeling with amino acids in cell culture) combined with mass spectrometry analysis. (nature.com)
  • We used consecutive orthogonal separation platforms to ensure sensitive detection: (a) ion-exchange of intact proteins, (b) SDS-PAGE separation of ion-exchange fractions and (c) HPLC separation of tryptic digests coupled to electrospray tandem mass spectrometry. (hindawi.com)
  • Absolute quantification with mass spectrometry and isotope labelled internal standards has found broad applications in biomedical research. (nist.gov)
  • These isotopes function as distinctive markers for compounds, detectable through techniques such as nuclear magnetic resonance (NMR) and mass spectrometry (MS). Commonly employed stable isotopes encompass 2H, 13C, and 15N. (blueweaveconsulting.com)
  • Modern mass spectrometry based analytical techniques play a crucial role in the discovery of metabolic alteration regulating ferroptosis by extensive analysis of metabolites such as the sulfhydryl residue containing cysteine metabolites, RTAs, and membrane phospholipids. (bmbreports.org)
  • We investigated the entire set of proteins modulated by BAG3 silencing in the human anaplastic thyroid 8505C cancer cells by using the Stable-Isotope Labeling by Amino acids in Cell culture strategy combined with mass spectrometry analysis. (oncotarget.com)
  • The issue of abundant (often housekeeping) proteins masking regulatory proteins of lower abundance (such as signaling proteins and cytokines) continues to be a challenging issue for proteomics particularly in the case of biofluids. (hindawi.com)
  • While modern proteomics experiments permit the analysis of hundreds to thousands of proteins in complex samples, the most powerful use of this data would combine information on protein activity with the identities of the active proteins. (hindawi.com)
  • Stable isotope tracing's role in understanding nutraceuticals' impact on cancer metabolism, as well as proteomics research, contributed to market expansion. (blueweaveconsulting.com)
  • Beyond metabolomics research, stable isotope-labeled compounds are experiencing increasing utilization in environmental analysis, proteomics, and medical diagnostic research. (blueweaveconsulting.com)
  • Improving the precision of quantitative bottom-up proteomics based on stable isotope-labeled proteins. (uni-frankfurt.de)
  • In the present study, an Escherichia coli strain, that overproduces the membrane-depolarizing toxin TisB, was established as a model for persistence by the use of high-throughput proteomics. (uni-giessen.de)
  • Subsequent investigation of the persister proteome during postantibiotic recovery by label-free quantitative proteomics identified proteins with importance to the recovery process. (uni-giessen.de)
  • Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics. (neb.com)
  • Discovery Proteomics and Absolute Protein Quantification Can Be Performed Simultaneously on an Orbitrap-Based Mass Spectrometer. (ncsu.edu)
  • We also examined the dataset of proteins obtained by the quantitative proteomics analysis using two tools, Downstream Effect Analysis and Upstream Regulator Analysis of the Ingenuity Pathways Analysis software. (oncotarget.com)
  • Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum. (unibas.ch)
  • Multiplexed proteomics is a powerful tool to assay cell states in health and disease, but accurate quantification of relative protein changes is impaired by interference from co-isolated peptides. (biorxiv.org)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
  • Quantitative targeted absolute proteomic analysis of 31 membrane proteins showed that P-glycoprotein (P-gp/mdr1a) protein expression levels were significantly increased in brain capillaries of PTZ (129%), EL (143%), and PHT mice (192%) compared with controls. (aspetjournals.org)
  • Proteomic approaches have proven powerful at identifying large numbers of proteins, but there are fewer reports of functional characterization of proteins in biological tissues. (hindawi.com)
  • Previous proteomic studies of intact platelets have collectively identified hundreds of proteins using a variety of fractionation strategies including 2-dimensional electrophoresis (2DE), multidimensional chromatographic separations, membrane prefractionation techniques, and adsorption to combinatorial hexapeptide ligand libraries [ 1 - 6 ]. (hindawi.com)
  • Lysosomal localization of the endogenous CLN7 protein has been demonstrated by proteomic analyses using purified human and rat tritosomes, by immunoblotting of mouse liver tritosomes and by immunohistochemical localization in cultured hippocampal neurons (15C18). (kentlandsinitiative.org)
  • In the present study, we provide quantitative proteomic data that have been obtained by mass spectrometric analysis of isolated lysosomes from Cln7 ko mouse fibroblasts by means of Stable Isotope Labelling by Amino acids in Cell culture (SILAC). (kentlandsinitiative.org)
  • Understanding K. phaffii (Pichia pastoris) Host Cell Proteins: Proteomic Analysis and Flow-through Affinity Clearance (T. I. Williams, Ed.). Biotechnology and Bioengineering, Vol. 01. (ncsu.edu)
  • Targeted capture of Chinese hamster ovary host cell proteins: Peptide ligand binding by proteomic analysis. (ncsu.edu)
  • CLN7 belongs to a group of atypical solute carriers of MFS type which are located at the plasma membrane and/or in intracellular compartments (14). (kentlandsinitiative.org)
  • We further observed mitochondria-released H2 O2 directly at the surface of the organelle and in the bulk cytosol, but not in the nucleus or at the plasma membrane, pointing to steep gradients emanating from mitochondria. (biomed.news)
  • Students will learn about protein expression and purification, and measurements of binding affinity and kinetics. (tamu.edu)
  • Protein Expression and Purification. (ezreregister.com)
  • This article provides an overview of the advances in protein expression and purification methodology over the past 40 years. (neb.com)
  • Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via "flow-through affinity chromatography" using peptide-based adsorbents. (ncsu.edu)
  • LP3 is fully equipped for recombinant protein production in E. coli and insect cells (BEVS) and protein purification using state of the art chromatography systems. (lu.se)
  • LP3 and PPS offer services for the entire process chain of production, purification, characterization, protein crystallisation and protein structure determination and structure refinement, or each individual step in the chain. (lu.se)
  • The Glasner Lab investigates mechanisms of protein evolution in order to understand how the biophysical properties of proteins influence their capacity to evolve new structures and functions. (tamu.edu)
  • My laboratory uses a combination of biochemical and biophysical techniques (NMR spectroscopy, X-ray crystallography, SAXS, and fluorescence spectroscopy) to understand the structure-dynamics-function relationships in these proteins. (tamu.edu)
  • Over 300 proteins were identified in the releasate, with a wide range of annotated biophysical and biochemical properties, in particular platelet activation, adhesion, and wound healing. (hindawi.com)
  • The respective protein-protein-interactions are characterized with biophysical, biochemical and cell biological methods. (hhu.de)
  • Various biophysical methods require large amounts of recombinantly expressed and purified proteins. (hhu.de)
  • Sphingolipids (SLs) are highly abundant components of cellular membranes and as such, are essentially involved in their biophysical and signaling properties. (encyclopedia.pub)
  • Depending on the length of their fatty acid chains and their degree of saturation, SL species have a strong impact on biophysical membrane parameters such as fluidity or rigidity and curvature, and on interaction with membrane proteins and/or cytoskeletal components, and membrane compartmentalization. (encyclopedia.pub)
  • As such, we provide services and make equipment available in the areas of recombinant protein production, crystallisation, biophysical characterization, and structure determination. (lu.se)
  • Following activation by agonists such as thrombin, platelets release storage granules and membrane vesicles that contain prothrombotic (e.g., fibrinogen), mitogenic (e.g., platelet derived growth factor), immunomodulatory (e.g., neutrophil-activating peptide 2), and adhesive (e.g., platelet endothelial cell adhesion molecule) proteins. (hindawi.com)
  • These toxins inhibit the release of neurotransmitters at the neuromuscular junction by cleaving soluble NSF attachment protein receptor (SNARE) complex proteins in nerve cells, which prevents the fusion of neurotransmitter containing vesicles to cell membranes 2 . (cdc.gov)
  • However, because of the unknown binding affinity of antibody to MRP2/Mrp2 protein across species and lack of purified MRP2/Mrp2 proteins for calibration, the immunoblotting assay was excluded from the absolute quantification of MRP2/Mrp2 protein for multiple species. (aspetjournals.org)
  • 2 , 4 The different quantification channels are encoded by the distribution of heavy isotopes between the reporter and balancer region of the reagents. (biorxiv.org)
  • However, because the masses of complementary ions are peptide-dependent and include the heavy isotope labels of the balancer-region (blue rectangles), they can be used for interference-free, accurate MS2 quantification. (biorxiv.org)
  • In addition, the effectiveness of antitoxin treatment may vary depending on circulating toxin levels, so that rapid identification and quantification of BoNT proteins in clinical specimens and food is desirable for optimization of treatment. (cdc.gov)
  • We describe the design and synthesis of a novel set of iso-baric tags for quantitative glycan profiling, which will have broad applications in carbohydrate based biomarker dis-covery, therapeutic protein characterization, and vaccine development. (nist.gov)
  • Through applying cutting edge techniques like advanced light microscopy, cell-free protein synthesis and microfluidics, we seek to systematic investigation of membrane proteins as well as bottom-up assembly of minimal cellular systems. (ezreregister.com)
  • Systems for the Cell-Free Synthesis of Proteins. (ezreregister.com)
  • To answer this question we evaluated the effect of 8 weeks of LCn-3PUFA supplementation (4 g·d-1 of Lovaza®) in nine 25-45 y old healthy subjects on the rate of muscle protein synthesis (by using stable isotope labelled tracer techniques) and the activation (phosphorylation) of elements of the mTOR-p70s6k pathway during basal, postabsorptive conditions and during a hyperinsulinemic-hyperaminoacidemic clamp. (ironmagazine.com)
  • Lipid synthesis requires bulk carbon skeleton acyl-CoAs, the transport of which depends on the acyl-CoA binding protein (ACBP). (biomedcentral.com)
  • Before entering lipid synthesis pathways, different kinds of acyl-CoA transporters which acts as the major carrier of acyl-CoAs, such as fatty acid binding protein (FABP), sterol carrier protein 2 (SCP2) and acyl-CoA-binding protein (ACBP), would activate and convert fatty acids to fatty acyl-CoA esters via a reaction catalyzed by fatty acyl-CoA synthetase and transported to various metabolic locations [ 11 ]. (biomedcentral.com)
  • Mammalian respiratory complex I (CI) is a 45-subunit, redox-driven proton pump that generates an electrochemical gradient across the mitochondrial inner membrane to power ATP synthesis in mitochondria. (biomed.news)
  • Inhibition of protein synthesis by TOR inactivation revealed a conserved regulatory mechanism of the BiP chaperone in Chlamydomonas. (unibas.ch)
  • Provision of dietary amino acids increases skeletal muscle protein synthesis (MPS), an effect that is enhanced by prior resistance exercise. (fasciaguide.com)
  • Co-translational insertion of cell-free expressed membrane proteins into lipid bilayers. (ezreregister.com)
  • MARS probes were also shown to successfully target the cell membrane, mitochondria, lysosomes, and other lipid structures within the cell. (columbia.edu)
  • Ferroptosis is well characterized by an iron-dependent accumulation of lipid peroxides and disruption of cellular membrane integrity. (bmbreports.org)
  • Transcriptional analysis of ACBP-deficient parasites revealed differentially expressed genes involved in a wide range of biological processes such as lipid metabolism, posttranslational modification, and membrane biogenesis. (biomedcentral.com)
  • In summary, depletion of multiple soluble lysosomal proteins suggest a critical role of CLN7 for lysosomal function, which may contribute to the pathogenesis and progression of CLN7 disease. (kentlandsinitiative.org)
  • Furthermore, dysregulated expression of several soluble lysosomal proteins and impaired macroautophagy in the Cln7 ko mice suggest that loss of Cln7 results in lysosomal dysfunction in the brain (19). (kentlandsinitiative.org)
  • Lysosomal function is critical for organismal homeostasis-mutations in genes encoding lysosomal proteins cause severe human disorders known as lysosomal storage diseases, and lysosome dysfunction is implicated in age-associated diseases including cancer, neurodegeneration and metabolic syndrome. (stanford.edu)
  • With a strict control for excluding thermal effects, we successfully recapitulated the non-thermal effects of THz radiation on the hydration on a protein surface ( Biophys J., 2021 ). (u-tokyo.ac.jp)
  • Columbia postdoc Dr. Yupeng Miao and colleagues published an article in 2021, summarizing their development of new MARS dyes that have different properties that are easier to synthesize and can visualize even more of the cell's proteins under the microscope at once! (columbia.edu)
  • LP3 is colocalised with and closely collaborates with the DEMAX platform of the ESS on production of deuterium labeled biomolecules (proteins, lipids) for neutron scattering experiments and the development of methods for crystal growth for neutron crystallography. (lu.se)
  • The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)
  • Labeling of TisB persisters with stable isotope-containing amino acids (pulsed-SILAC) revealed an active translational response to ampicillin, including several RpoS-dependent proteins. (uni-giessen.de)
  • Major growth drivers for the global stable isotope labeled compounds market include an increasing surge in research activities and growing trend in analytical research within the pharmaceutical and biotechnology sector. (blueweaveconsulting.com)
  • The rising incidence of cancer has notably boosted analytical investigations using stable isotope-labeled compounds. (blueweaveconsulting.com)
  • However, high cost of stable isotope labeled compounds and lack of skilled professionals are anticipated to restrain the overall market growth during the forecast period. (blueweaveconsulting.com)
  • Researchers, particularly those engaged in metabolism-mediated toxicity studies, have shown significant interest in stable isotope-labeled compounds. (blueweaveconsulting.com)
  • COVID-19 pandemic had a positive impact on the global stable isotope labeled compounds market. (blueweaveconsulting.com)
  • During the pandemic period, the global stable isotope labeled compounds market experienced noteworthy growth due to heightened demand for stable isotope-labeled compounds, driven by their crucial role in research related to the virus, such as investigations into the structure and dynamics of membrane proteins in pathogens like SARS-CoV-2. (blueweaveconsulting.com)
  • The lessons learned and the increased utilization of stable isotope-labeled compounds during the pandemic are likely to contribute to healthy market expansion during the forecast period. (blueweaveconsulting.com)
  • On the basis of end user, the global stable isotope labeled compounds market is divided into Academic & Research Institutions, Hospital & Diagnostic Centers, and Pharmaceutical & Biotechnology Companies segments. (blueweaveconsulting.com)
  • The pharmaceutical & biotechnology companies segment is expected to hold the highest share in the global stable isotope labeled compounds market by end user during the forecast period. (blueweaveconsulting.com)
  • The light chain is a zinc-metalloprotease which is responsible for the cleavage of three SNARE complex proteins including SNAP-25 (Synaptosome-associated protein of 25 kDa), synaptobrevin-2 (also termed VAMP-2) and syntaxin. (cdc.gov)
  • Transient, reversible effects of the THz radiation to the protein structures were encoded in NMR signal intensities via proton exchange with solvent water. (u-tokyo.ac.jp)
  • Light-induced Printing of Protein Structures on Membranes in vitro. (ezreregister.com)
  • For example, MARS probes were used to image subcellular structures including the protein alpha-tubulin, which is a protein within microtubules that provide structural support to the cell, as well as fibrillarin, which is a protein that is used as a nucleoli marker. (columbia.edu)
  • Structural comparisons of active CI structures from our decylubiquinone-NADH and rotenone-NADH datasets reveal that Q10 reduction at site 1 is not coupled to proton pumping in the membrane arm, which might instead be coupled to Q10 oxidation at site 2. (biomed.news)
  • Each individual composition results from an extensive analysis of the crystallization conditions that have yielded membrane protein structures so far. (jenabioscience.com)
  • For this purpose we examine their 3D structures and their interactions with host cellular proteins or other virus proteins. (hhu.de)
  • Additionally, preparation of stable isotope labeled protein is a prerequisite for elucidating their three-dimensional structures by NMR. (hhu.de)
  • LP3 can further process data, solve and refine protein structures. (lu.se)
  • The summer research project in the Gohil lab will focus on discovering evolutionarily conserved proteins that are required for mitochondrial energy metabolism. (tamu.edu)
  • Protein and peptide chromatography prior to MS analysis can partly address this issue. (hindawi.com)
  • Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests. (ncsu.edu)
  • Removal of host cell proteins from cell culture fluids by weak partitioning chromatography using peptide-based adsorbents. (ncsu.edu)
  • Each serotype of BoNT hydrolyzes their protein substrates at different peptide bonds. (cdc.gov)
  • We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)
  • A number of bleeding disorders arise as a result of mutations in the genes for proteins involved in platelet aggregation. (hindawi.com)
  • Integrated epigenetics of human breast cancer : synoptic investigation of targeted genes, microRNAs and proteins upon demethylation treatment. (unibas.ch)
  • Expression analyses showed that the mRNA expression, biosynthesis, intracellular sorting and proteolytic processing of Cln5 were not affected, whereas the depletion of mature Cln5 protein was due to increased proteolytic degradation by cysteine proteases in Cln7 ko lysosomes. (kentlandsinitiative.org)
  • Lysosomes are membrane-bound compartments that degrade macromolecules and clear damaged organelles to enable cellular adaptation to various metabolic states. (stanford.edu)
  • For example, a protein having multiple membrane spanning domains might not properly insert into membrane bilayers of the heterologous host or a protein might not be expressed in a soluble form. (neb.com)
  • The bilayers are labelled with a rhodamine phospholipid (red). (lu.se)
  • We put efforts on developing methodologies that can elucidate the functional mechanisms of high-molecular-weight proteins, establish structure-function relationship analyses that do not rely on mutants, and introduce intracellular structure-guided drug development. (u-tokyo.ac.jp)
  • The topics of the group are structural and functional aspects of viral proteins with the main focus on accessory and regulatory proteins of viruses. (hhu.de)
  • His research has covered all aspects of human nutrition, with a strong focus on the use of stable isotopes to determine body composition and functional capability at the level of individual tissues and the whole body. (southampton.ac.uk)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
  • Proteins identified within the fraction with monocyte promigratory activity and not in other inactive fractions included vimentin, PEDF, and TIMP-1. (hindawi.com)
  • and intraclonal fractions differing in surface membrane (sm) IgM and IgD densities. (bvsalud.org)
  • To demonstrate the power of TMTProC, we analyzed a human and yeast interference sample and were able to quantify 13,290 proteins in 24 fractions. (biorxiv.org)
  • Lipids are the major structural elements of all biological membranes, serving as signaling molecules within and between cells. (biomedcentral.com)
  • Background: In this study, we identify the nature of the immunological response of human peripheral blood mononuclear cells (PBMC) and lamina propria gastric lymphocytes (LPL) to two antigens, the neutrophil activating protein (NapA) and alkyl hydroperoxide reductase (AphC). (bios-mep.info)
  • Among them, AhpF, a component of alkyl hydroperoxide reductase, and the outer membrane porin OmpF were found to affect the persistence time of TisB persisters. (uni-giessen.de)
  • Botulinum neurotoxins (BoNTs) are proteases that cleave specific cellular proteins essential for neurotransmitter release. (cdc.gov)
  • BoNTs are zinc metalloproteases that cleave and inactivate specific cellular proteins essential for the release of the neurotransmitter acetylcholine ( Figure 1 ). (cdc.gov)
  • The LPS outer membrane of H pylori is a less potent inducer of the host complement cascade. (medscape.com)
  • To make this practical, mechanistic insights into the effects of THz radiation on proteins would be prerequisite. (u-tokyo.ac.jp)
  • 2 , 3 A key feature of apoptosis has been defined as the maintenance of an intact cellular membrane (detectable as exclusion of impermeable dyes such as propidium iodide, PI) throughout the apoptotic program to prevent intracellular protein release and subsequent immunological activation. (nature.com)
  • Defining the core proteome of the chloroplast envelope membranes. (uni-frankfurt.de)
  • Our analyses confirm the association of the proteome profile observed in BAG3-silenced cells with an increase in cell survival and a decrease in cell proliferation and invasion, and highlight the possible involvement of four tumor suppressor miRNAs and TP53/63 proteins in BAG3 activity. (oncotarget.com)
  • The purpose of this study was to demonstrate experimentally that alterations of in vivo transporter function at the blood-brain barrier (BBB) in disease and during pharmacotherapy can be reconstructed from in vitro data based on our established pharmacoproteomic concept of reconstructing in vivo function by integrating intrinsic transport activity per transporter molecule and absolute protein expression level at the BBB. (aspetjournals.org)
  • 17.Rongrong Ma, Zhong Yang, Lei Huang, Xiangcheng Zhu, Lei Kai, Jin Cai, Xiaoning Wang, Zhinan Xu Construction of an efficient Escherichia coli cell-free system for in vitro expression of several kinds of proteins. (ezreregister.com)
  • The recombinant NcACBP protein was expressed to verify the binding activities of NcACBP in vitro , and the heterologous expression of NcACBP in Δacbp yeast in vivo . (biomedcentral.com)
  • The NcACBP recombinant protein was able to specifically bind acyl-CoA esters in vitro . (biomedcentral.com)
  • We developed an experimental scheme to analyze the THz-induced changes in structure and dynamics of proteins in a residue-specific manner. (u-tokyo.ac.jp)
  • We now show that during early apoptosis limited membrane permeabilization occurs in blebs and apoptotic bodies, which allows release of proteins that may affect the proximal microenvironment before the catastrophic loss of membrane integrity during secondary necrosis. (nature.com)
  • Complex biological samples have also been successfully separated in multiple dimensions at the protein level. (hindawi.com)
  • Manipulation of protein functions aids both biological research and disease treatments. (u-tokyo.ac.jp)
  • LP3 is the only academic protein production platform in Sweden providing specialisation in protein production using the baculovirus expression vector system (BEVS, insect cells) and perdeuteration of biological macromolecules. (lu.se)
  • In NMR analyses of proteins, the atoms of interest can be selectively observed by labeling appropriate atoms with stable isotopes such as 2 H, 13 C, and 15 N. Especially, high-level deuteration of protons surrounding observed atoms enables sensitive detection of NMR signals in combination with the TROSY methods. (u-tokyo.ac.jp)
  • Consequently, a lot of effort is put into identifying the constituent proteins using mass spectrometric methods. (lu.se)
  • In vivo P-gp/mdr1a function at the BBB was reconstructed from the measured P-gp/mdr1a protein expression levels and intrinsic transport activity for verapamil per P-gp/mdr1a previously reported by our group. (aspetjournals.org)
  • The isotope labeling strategy has been developed in E. coli expression systems, however, it is not that straight forwards in eukaryotic cells, such as insect cells and mammalian cells. (u-tokyo.ac.jp)
  • Co-translational stabilization of insoluble proteins in cell-free expression systems. (ezreregister.com)
  • Many proteins are extremely difficult to express in heterologous expression systems. (neb.com)
  • For example, in many experimental scenarios expression of a protein originating from a higher eukaryote is being produced in a bacterium where factors such as codon usage, translation rate, and redox potential are significantly different. (neb.com)
  • Additionally, inherent properties of the target protein may represent challenges for the expression host. (neb.com)
  • Finally, many proteins require post-translational modifications (e.g. glycosylation or phosphorylation) that are absent or significantly different from expression host to expression host. (neb.com)
  • There is no single solution for the expression of all classes of difficult proteins. (neb.com)
  • For microbial expression systems, these solutions often come in the form of unique host strains that have been genetically modified to enhance the production of a certain difficult protein class. (neb.com)
  • Other expression solutions seek to address problems by controlling aspects of how a target protein is produced. (neb.com)
  • Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery. (neb.com)
  • Therefore, we construct and use efficient recombinant expression systems for the proteins of our interest. (hhu.de)
  • Thus, altered or deregulated platelet function underpins many diseases, and platelet proteins are potential targets for novel therapeutic agents. (hindawi.com)
  • Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach. (ncsu.edu)
  • The presence of PEDF and involucrin, two proteins not previously reported in platelet releasate, was confirmed by western blotting. (hindawi.com)
  • For the design, they used a similar foundation to the previous MARS probes, but included some adjustments like changing the core atom or substituting stable isotopes throughout the molecule. (columbia.edu)
  • We have developed a strategy to selectively introduce methyl 13 C-labeling with the high-level deuteration of surrounding amino acid residues in insect cells. (u-tokyo.ac.jp)
  • We have identified a cytosolic acyl-CoA binding protein, with highly conserved amino acid residues and a typical acyl-CoA binding domain in N. caninum . (biomedcentral.com)
  • It is the least abundant of the stable halogens , being the sixty-first most abundant element. (wikipedia.org)
  • In addition to maintenance of membrane integrity, apoptotic cells can be discriminated from viable counterparts based on several morphological hallmarks, including cell contraction, nuclear condensation and fragmentation, and actomyosin contraction-dependent membrane blebbing and apoptotic body formation. (nature.com)
  • The bacteriocin Angicin interferes with bacterial membrane integrity through interaction with the mannose phosphotransferase system. (uni-ulm.de)
  • The cross-reactions of MRP2/Mrp2 protein of different species with anti-human MRP2 polyclonal antibody were found by Western blotting. (aspetjournals.org)
  • Endogenous testosterone released from the sample by ANS (8-anilino-1-naphthalene sulfonic acid) and norgestrel competes with the added testosterone derivative labeled with ruthenium complex for the binding sites on the biotinylated antibody. (cdc.gov)
  • 1st incubation: 50 µL of sample is incubated with a testosterone- specific biotinylated antibody and a testosterone derivative labeled with a ruthenium complex. (cdc.gov)
  • The binding sites of the labeled antibody become occupied partly by the sample analyte (depending on its concentration) and partly by the ruthenium-labeled hapten to form the respective immunocomplexes. (cdc.gov)
  • These proteins may modulate the interaction of platelets with their local cellular environment. (hindawi.com)
  • Application of electromagnetic waves at around terahertz (THz) frequency to proteins could be game-changing, as the THz radiation could modulate a protein function if it resonates with functionally relevant vibrational modes of the protein. (u-tokyo.ac.jp)
  • Botulinum neurotoxins (BoNTs) are bacterial proteins that cause the life-threatening disease botulism 1 . (cdc.gov)
  • BoNTs are dichain proteins consisting of a light chain of 50 kDa and a heavy chain of 100 kDa linked by a disulfide bond 5 . (cdc.gov)
  • Furthermore, XCN 1 is secreted by an ABC transporter TolC-like protein complex and is thought to be involved in killing microbes living inside the insect gut or other bacterial food competitors during the infection cycle and the nematode development. (uni-frankfurt.de)
  • Astonishingly, homologues of the membrane-bound and D-asparagine-specific peptidase (XcnG) and the encoding NRPS for the starting module (XcnA) for the acylated D-asparagine residue were identified in many different bacterial genera. (uni-frankfurt.de)
  • Dying cells have been defined as apoptotic by distinguishing features, including cell contraction, nuclear fragmentation, blebbing, apoptotic body formation and maintenance of intact cellular membranes to prevent massive protein release and consequent inflammation. (nature.com)
  • High Level Cell-Free Production of Membrane Proteins into Nanodiscs. (ezreregister.com)
  • Artificial Environments for the Co-Translational Stabilization of Cell-Free Expressed Proteins. (ezreregister.com)
  • Assessing the role of AhpF and OmpF in TisB-independent persisters demonstrated that the importance of a particular protein for the recovery process strongly depends on the physiological condition of a persister cell. (uni-giessen.de)
  • Indeed, they expanded the list of probes by developing 30 new molecules that are able to label specific cell organelles and functions. (columbia.edu)
  • We also measured the concentrations of protein, RNA, and DNA in muscle to obtain indices of the protein synthetic capacity, translational efficiency and cell size. (ironmagazine.com)
  • In addition, the muscle protein concentration and the protein-to-DNA ratio (i.e., muscle cell size) were both greater (P (ironmagazine.com)
  • 2016) Cell, C9orf72 dipeptide repeats impair the assembly, dynamics and function of membrane-less organelles. (umn.edu)
  • Translocating the CagA protein into the gastric epithelial cells causes rearrangement of the host cytoskeleton and alters cell signaling and perturbs cell cycle control. (medscape.com)
  • In a typical REU project, students analyze sequence data to identify mutations that could influence the evolution of protein function, then construct the mutations, express, purify and assay the enzymes to determine the role of the mutated position. (tamu.edu)
  • Plasma has a significant dynamic range, with more than 10 orders of magnitude separating albumin concentration and the rarest measurable proteins identified to date [ 10 ]. (hindawi.com)
  • The JBScreen Membrane crystallization conditions are primarily ordered by type and concentration of the precipitant. (jenabioscience.com)
  • A Diffusion Model to Quantify Membrane Repair Process in Listeria monocytogenes Exposed to High Pressure Processing Based on Fluorescence Microscopy Data. (uni-ulm.de)
  • While the locations and inferred H-bond connec- water molecules in a membrane protein, despite tivities of these internal water molecules are essen- being deeply buried and extensively H-bonded to tial, one would also like to quantify their mobility. (lu.se)
  • La información más reciente sobre el nuevo Coronavirus de 2019, incluidas las clínicas de vacunación para niños de 6 meses en adelante. (stanfordchildrens.org)