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  • antigen
  • Recently, we cloned and expressed a 44-kDa major antigen of the HGE agent and demonstrated that this recombinant antigen (rP44) may be more specific for serodiagnosis of HGE than whole organism-infected cells due to the absence of heat shock proteins or other antigenically cross-reactive proteins ( 17 ). (asm.org)
  • LipL32 is known to be the most prominent protein in the leptospiral protein profile and is an immunodominant antigen during human leptospirosis ( 10 , 14 , 17 ). (asm.org)
  • The immune response against the Plasmodium falciparum variant surface antigen P. falciparum erythrocyte membrane protein 1 (PfEMP1) is a key component of clinical immunity against falciparum malaria. (edu.au)
  • genes
  • Considering previous findings that (a) AtPQL4 and AtPQL6 are localised in the ER (b) ATPQL mutants show differential expression of genes involved in the unfolded protein response (UPR) and (c) over-expression of AtPQL4 and AtPQL6 impacts on the targeting of other proteins, the observed phenotype could be linked to the unfolded protein response and autophagy that occurs during sugar starvation. (gla.ac.uk)
  • Membrane proteins, which consist of 20~30% of total encoding genes in the genome, play a critical role in multiple biological processes, including ligand-receptor binding, signal transduction, molecule transportation, intercellular recognition, and cell adhesion. (creative-biolabs.com)
  • thesis
  • All proteins studied within this thesis are localized in the chloroplast, the organelle that originated from cyanobacteria, in which plants and algae convert the energy from sunlight into carbohydrates in the process called photosynthesis. (diva-portal.org)
  • In this thesis, data identifying one PSII LMW protein, PsbY, as a chaperone for the PSII subcomplex Cytochrome b 559 are presented. (diva-portal.org)
  • novel
  • The discovery of novel muscle-specific, membrane proteins would likely provide insight into mechanisms of disease and potentially yield new therapeutic targets. (ahajournals.org)
  • Here, significant progress is presented towards solving the high-resolution crystal structure of the novel amyloid transporter FapF from Pseudomonas , which facilitates the secretion of the amyloid-forming polypeptide FapC across the bacterial outer membrane. (imperial.ac.uk)
  • In summary, our study provides a novel approach to obtain direct information on the peripheral and integral protein composition of the IAM and consolidates IAM38 as a genuine secondary sperm-zona binding protein. (queensu.ca)
  • With a full understanding of the critical requirements in this field, Creative Biolabs has developed a novel Magic™ platform, which enables high-efficient expression of membrane proteins and following antibody development routes based on our multiple impeccable technologies. (creative-biolabs.com)
  • Arabidopsis
  • Our data show that FtsH11 has an influence on the structure and function of chloroplasts of Arabidopsis plants grown under continuous light along with protein import into the same. (diva-portal.org)
  • Here, we use the heterologous expression of a plant potassium channel, the Arabidopsis thaliana guard cell outward-rectifying K+ channel, AtGORK (At5G37500) in HEK-293 cells as an example, to evaluate commonly used transfection reagents and fluorescent detection methods, and provide a detailed methodology for optimized transient transfection and expression of membrane proteins for in vivo studies in general and for single-cell applications in particular. (edu.sa)
  • mammalian
  • AtPQL4 and AtPQL6 show high amino acid sequence identity between each other and they are the closest A. thaliana homologues of the mammalian LEC35/MPDU1 protein, which has been shown to be required for all types of Man-P-Dol dependent glycosylation in the ER. (gla.ac.uk)
  • The outer membrane (OM) of the mammalian pathogen Leptospira kirschneri was isolated in the form of membrane vesicles by alkaline plasmolysis and separated from the protoplasmic cylinder by sucrose density gradient ultracentrifugation. (asm.org)
  • During mammalian fertilization, the exposure of the inner acrosomal membrane (IAM) after acrosomal exocytosis is essential for the secondary binding between sperm and zona pellucida (ZP) of the oocyte, a prerequisite for sperm penetration through the ZP. (queensu.ca)
  • lipid
  • Recently, cell-free expression systems for membrane proteins have been developed that incorporate lipid nanodiscs consisting of membrane scaffold proteins (MSPs, derived from apolipoproteins) that wrap around a patch of phospholipid bilayer. (plantae.org)
  • As most membrane proteins are partially or completely embedded in the lipid bilayer, traditional expression methods meet obstacles in presenting the native conformations, such as low expression level, difficult extraction, misfolding, and incomplete post-translational modifications. (creative-biolabs.com)
  • eukaryotic
  • Thus, because of their involvement in multiple key aspects of cellular metabolism, cysteine residues in proteins should be widely distributed across all organisms, from viruses to complex forms like the eukaryotic cells. (biomedcentral.com)
  • biological
  • They carry out diverse biological functions, including pore formation, membrane anchoring, enzyme activity, and are often responsible for bacterial virulence. (uic.edu)
  • porin
  • were initially identified in the early 1980s by selective extraction techniques and classified according to their apparent molecular mass as 36-38 kDa OMPs or group 2 porin proteins and 31-34 kDa and 25-27 kDa OMPs which belong to the group 3 proteins. (nih.gov)
  • 85%), encode the 36 kDa porin proteins, now named Omp2a and Omp2b proteins respectively. (nih.gov)
  • residues
  • In both proteins, we found that the CX 2 CX 10 CX 2 C motif chelated a zinc ion via its cysteine residues, but the sphere of coordination was remarkably different in each case. (biomedcentral.com)
  • Experiments involving site-directed mutagenesis, SDS-PAGE, CD spectroscopy, tryptophan flourescence, thermal and chemical denaturation have confirmed predictions of important residues in the protein-protein interaction interface of OmpF, VDAC, and Tom40 proteins. (uic.edu)
  • ligand
  • Online size‐exclusion high‐performance liquid chromatography light scattering and differential refractometry methods to determine degree of polymer conjugation to proteins and proteinprotein or protein‐ligand association states. (currentprotocols.com)
  • The importance of the in solution characterisation of the conformational behaviour and ligand binding properties of proteins in both far- andnear-UV regions and the use of high-throughput CD (HT-CD) using 96- and 384-well multiplates to study the folding effects in various protein crystallisation buffers are also discussed. (springer.com)
  • receptor
  • Rather, it appeared that the polysaccharide was first bound to a putative outer membrane receptor complex and then translocated into the periplasm, where the degradative enzymes were located. (asm.org)
  • FERONIA is a plant receptor-like kinase and part of a very large but not well characterized family of membrane proteins. (plantae.org)
  • The sulphonylurea receptor SUR1 regulates ATP-sensitive mouse Kir6.2 K+ channels linked to the green fluorescent protein in human embryonic kidney cells (HEK 293). (edu.sa)
  • exocytosis
  • However, both Sec1p and Munc18a are required factors for exocytosis and stimulate in vitro membrane fusion of the appropriate preassembled t-SNARE complex and v-SNARE. (rice.edu)
  • recombinant
  • MAbs 5C11 and 5D13 recognized the recombinant 44-kDa protein by Western immunoblot analysis, but MAb 3E65 did not. (asm.org)
  • homologous
  • MAb 3E65 reacted with a 44-kDa protein in the homologous human isolate but not in the remaining five isolates. (asm.org)
  • enzyme
  • Human granulocytic ehrlichiosis (HGE) is an emerging tick-borne zoonosis characterized by fever, headache, myalgia, elevated liver enzyme (serum aminotransferase) levels, leukopenia, anemia, thrombocytopenia, and elevated C-reactive protein levels ( 2 ). (asm.org)