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  • surface proteins
  • University of Minnesota researchers have discovered this previously unknown signaling pathway that regulates surface proteins on bacteria that can lead to new targets for antibiotics. (phys.org)
  • Although the overall turnover of surface proteins was biphasic in nature, the rapid phase of protein loss was largely due to low molecular weight species. (pnas.org)
  • encode
  • Eight exons of LMP2 isoforms encode 12 membrane spanning segments that are connected by short hydrophilic loops and ends with a 27 amino acid cytoplasmic C-terminus domain. (wikipedia.org)
  • solubilization
  • In particular, isolation procedures needed for in-depth studies of an individual type of membrane protein is complicated by the high tendency of membrane proteins to lose function upon detergent-based solubilization procedures. (chalmers.se)
  • This demanding challenge has spurred intense activities directed towards membrane-protein separation and enrichment protocols applicable directly on natural cell-membranes, with the aim to bypass the destructive solubilization step. (chalmers.se)
  • molecular
  • How proteins fold is a fundamental problem in molecular biology and has been the subject of intense study. (pnas.org)
  • R.R. Dubreuil , Molecular and Genetic Dissection of the Membrane Skeleton in Drosophila. (elsevier.com)
  • The atomic model provides a possible molecular explanation to a longstanding puzzle in physiology-how membranes can be freely permeable to water but impermeable to protons. (shapeways.com)
  • The turnover of most proteins of molecular weight above 50,000 was very slow (t1/2 greater than 80 hr) and was rather synchronous. (pnas.org)
  • Frey, A. (1993) "Gamma-Glutamyl Transpeptidase: Molecular Cloning and Structural and Functional Features of a Blood-Brain Barrier Marker Protein" in: The Blood-Brain Barrier: Cellular and Molecular Biology (ed. (springer.com)
  • Experience in molecular / cell biology techniques / protein expression is strongly desirable. (weizmann.ac.il)
  • Many functions of the cell membrane are controlled by proteins that transduce signals across the lipid bilayer, either via molecular-transport reactions or structural rearrangements. (chalmers.se)
  • Continuous Lipid Bilayers Derived from Cell Membranes for Spatial Molecular Manipulation. (chalmers.se)
  • electron microscopy
  • Collaborate closely with imaging tool builders at Janelia to develop super-resolution and electron microscopy pipeline to map and annotate the sub-cellular localization of membrane proteins in the brain. (nature.com)
  • Degradation
  • Cédric Pety de Thozée and Michel Ghislain, "ER-Associated Degradation of Membrane Proteins in Yeast," TheScientificWorldJOURNAL , vol. 6, pp. 967-983, 2006. (hindawi.com)
  • Recent studies in yeast have highlighted the possible function of the Sar1p/COPII machinery in ER-associated degradation of some lumenal and membrane proteins. (hindawi.com)
  • proteome
  • Integral membrane proteins constitute a significant fraction of the proteome of all living cells. (neb.com)
  • Mapping the human membrane proteome: a majority of the human membrane proteins can be classified according to function and evolutionary origin. (wikipedia.org)
  • Shevchenko G, Sjo MOD, Malmstro D, Wetterhall M, Bergquist J (2010) Cloud-point extraction and delipidation of porcine brain proteins in combination with bottom-up mass spectrometry approaches for proteome analysis. (springer.com)
  • GPCR
  • This procedure has been extensively used for ligand - G protein-coupled receptors (GPCR) and their complexes. (wikipedia.org)
  • peptides
  • Polypeptide toxins and many antibacterial peptides , such as colicins or hemolysins , and certain proteins involved in apoptosis , are sometimes considered a separate category. (wikipedia.org)
  • homologous
  • Homology modeling can be used to construct an atomic-resolution model of the "target" integral protein from its amino acid sequence and an experimental three-dimensional structure of a related homologous protein. (wikipedia.org)
  • stability
  • The thermodynamic stability of proteins is typically measured at high denaturant concentrations and then extrapolated back to zero denaturant conditions to obtain unfolding free energies under native conditions. (pnas.org)
  • We therefore sought to measure stability under native conditions, using a method that does not perturb the properties of the membrane or membrane mimetics. (pnas.org)
  • Structural proteins are attached to microfilaments in the cytoskeleton which ensures stability of the cell. (phys.org)
  • sequence
  • For transmembrane entities, all members of the sequence cluster (90% sequence identity) are programmatically infered to be members of the same class of transmembrane proteins by applying the above procedure for single entity mpstruc entries. (rcsb.org)
  • immune
  • For example, proteins involved in immune response . (wikipedia.org)
  • The cells were then incubated with rabbit anti-dinitrophenyl IgG and the immune complexes formed with the trinitrophenyl-proteins were isolated from detergent lysates of the cells by using fixed Staphylococcus aureus as the immunoadsorbent. (pnas.org)
  • covalently
  • interaction by a covalently bound membrane lipid ( lipidation ) 4. (wikipedia.org)
  • bR contains a covalently bound retinal chromophore that complicates unfolding analysis because it can slowly hydrolyze off in the SDS unfolded protein ( 12 ). (pnas.org)
  • structure
  • Compared to other classes of proteins, the determination of membrane protein structure s has remained a challenge in large part due to the difficulty in establishing experimental conditions where the correct conformation of the protein in isolation from its native environment is preserved. (wikipedia.org)
  • For membrane proteins, the extrapolations are fraught with considerable uncertainty as the denaturants may have complex effects on the membrane or micellar structure. (pnas.org)
  • extraction
  • Hi netters I have specific problem in extraction of membrane protein. (bio.net)
  • In this chapter we provide an analytical approach that includes cloud-point extraction (CPE) of total membrane proteins with the non-ionic detergent Triton X-114 and subsequent analysis of their N -glycans using hydrophilic interaction liquid chromatography (HILIC)-UPLC/HPLC. (springer.com)
  • Donoghue PM, Hughes C, Vissers JPC, Langridge JI, Dunn MJ (2008) Nonionic detergent phase extraction for the proteomic analysis of heart membrane proteins using label-free LC-MS. Proteomics 8(18):3895-3905. (springer.com)
  • targets
  • Potential targets for vaccine development are H. pylori-specific proteins that are surface-exposed and highly antigenic. (ebi.ac.uk)
  • An evidence of their important role is the fact that they are the targets of more than half of today's drugs, and significant effort has therefore been devoted to the development of bioanalytical instruments capable of measuring membrane-protein controlled reactions. (chalmers.se)
  • coli
  • These proteins have definetly been expressed in Xenopus oocytes and in yeast, I am not sure about E. coli (the beta subunit is a glycoprotein). (bio.net)
  • Hi, I am trying to express in E. coli four different membrane proteins (ABC transporter) in either a pET vector with an N-terminal 6x his-tag or an pASK-IBA vector (tet promotor) with N- or C-terminal strep-tag, respectively. (bio.net)
  • When using E. coli as a host, it is advantageous to express membrane proteins in moderation to avoid saturation of the membrane protein biogenesis pathway, which may lead to cell death and/or inclusion body formation. (neb.com)