• Cell viability and cytotoxicity assays are used for drug screening and cytotoxicity tests of chemicals. (dojindo.com)
  • WSTs produce water-soluble formazans and are suitable for assays of cell proliferation and cytotoxicity. (dojindo.com)
  • Therefore, CCK-8 is stable for at least 6 months at room temperature and one year at 0-5°C. Since WST-8, WST-8 formazan, and 1-Methoxy PMS have no cytotoxicity in cell culture media, additional experiments may be carried out using the same assay plate. (dojindo.com)
  • Cytotoxicity LDH Assay Kit-WST is used for cytotoxicity determination by measuring the activity of lactate dehydrogenase (LDH) released. (dojindo.com)
  • Learn about the different types of cytotoxicity assay. (abcam.com)
  • We also deliver products for the detection of cathepsin, oxidative stress, fluorescent enzymatic assays, metabolic assays, ultra sensitive cholinesterase & ELISA products, and cytotoxicity luminescence kits. (biosciregister.com)
  • Cytotoxicity potential of PAP was analysed utilizing MTS assay. (aabioetica.org)
  • LDH toxicity analysis kit (cytotoxicity)-- determine the LDH,LDH released by damaged cells can formulate pyruvate and NADH by catalyze lactic acid, and NADH can transfer tetrazolium salt MTT to formazan by electronic carrier. (abbkine.com)
  • Cytotoxicity assays are widely used by the pharmaceutical industry to screen for cytotoxicity in compound libraries. (wikipedia.org)
  • Cytotoxicity can also be monitored using the 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) or with 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), which yields a water-soluble product, or the MTS assay. (wikipedia.org)
  • Cytotoxicity can also be measured by the sulforhodamine B (SRB) assay, WST assay and clonogenic assay. (wikipedia.org)
  • A collapse in mitochondrial membrane and redox potential may induce unwanted loss of cell viability and be a cause of various pathologies. (stratech.co.uk)
  • Although the 3[H]thymidine-uptake assay is regarded as a reliable cell viability assay, the use of a radioisotope causes various concerns. (dojindo.com)
  • Cell Counting Kit-F is another cell viability assay kit based on the esterase activity of viable cells. (dojindo.com)
  • These esterase substrates, therefore, can serve as viability assay probes for cells. (dojindo.com)
  • Learn about cell viability assays. (abcam.com)
  • Since 1994, ImmunoChemistry Technologies has been producing top quality reagents, kits, and services for cell viability and ELISA applications in a broad range of research areas. (immunochemistry.com)
  • Assessing cell membrane integrity is one of the most common ways to measure cell viability and cytotoxic effects. (wikipedia.org)
  • In addition to using dyes to indicate the redox potential of cells in order to monitor their viability, researchers have developed assays that use ATP content as a marker of viability. (wikipedia.org)
  • The FLIPR® Membrane Potential Assay Kits deliver homogenous fluorescence-based formulations for observation of real-time membrane potential changes associated with ion channel activation and ion transporter proteins. (moleculardevices.co.jp)
  • Cells' status can be inferred by examining autophagy proteins such as ATG8, as well as measuring ROS and mitochondrial membrane potential. (nature.com)
  • Mechanistically, both peptides caused a significant reduction at G0/G1 phase, in correlation with an augmented expression of the cell cycle inhibitory proteins p53, p27, p21, necrosis, exacerbated generation of reactive oxygen species and diminished mitochondrial membrane potential, all hallmarks of cellular stress. (nature.com)
  • Discover our full range of catalog products including Labeling and Detection, Peptides, Reagents for peptides synthesis, Assay Kits and Proteins. (anaspec.com)
  • Also, proteins and other contaminants from hands may bind to the membranes and cause background. (thermofisher.com)
  • Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. (anaspec.com)
  • Active caspases can then cleave many proteins, including poly-ADP ribose polymerase (PARP), other caspases and other protein substrates en masse. (bdbiosciences.com)
  • Useful tips for selecting caspase substrate-based activity assays. (abcam.com)
  • BD Biosciences also offers a range of tools for caspase activity assays from individual fluorogenic peptide substrates and inhibitors, to kits, to ready-to-use assay plates. (bdbiosciences.com)
  • We offer a wide assortment of fluorescent probes for analyzing aspects of normal mitochondrial activity in live cells, including reactive oxygen species (ROS) production, mitochondrial membrane potential and calcium flux. (stratech.co.uk)
  • Reactive oxygen species (ROS) and mitochondrial membrane potential had been measured by DCF-DA and JC10 staining, respectively. (aabioetica.org)
  • ICT offers a line of in vitro cell-based assay kit products that enable researchers to measure changes in the levels of reactive oxygen species and nitric oxide synthase in living cells. (immunochemistry.com)
  • In this presentation, learn how to detect the presence of oxidatively damaged DNA, quantify levels of reactive oxygen species such as hydrogen peroxide and nitric oxide, as well as how to monitor mitochondrial membrane potential. (immunochemistry.com)
  • Elevated levels of solute carrier family 14 member 1 expression induced mitochondrial reactive oxygen species accumulation, diminished the intracellular adenosine triphosphate level, and destroyed both mitochondrial membrane potential integrity and mitochondrial morphology. (biomedcentral.com)
  • This study in a tertiary care hospital in Egypt used real-time PCR assay to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the blaKPC gene and compared the results with modified Hodge test. (who.int)
  • The kits employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. (moleculardevices.co.jp)
  • With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. (anaspec.com)
  • The currently available serum tryptase assay (ImmunoCap Tryptase, Phadia Laboratory Systems, Uppsala, Sweden) uses quantitative fluorescence to determine serum tryptase levels. (medscape.com)
  • The Caspase-5 Colorimetric Assay Kit is designed to provides a simple and convenient means for assaying the activity of caspases that recognize the sequence WEHD. (celltechgen.com)
  • BioVision's newly designed ADP Assay Kit provides a convenient colorimetric and fluorometric means to measure ADP level. (celltechgen.com)
  • This assay measures the reducing potential of the cell using a colorimetric reaction. (wikipedia.org)
  • Label-free real-time techniques provide the kinetics of the cytotoxic response rather than just a snapshot like many colorimetric endpoint assays. (wikipedia.org)
  • Because ion channel activity is highly sensitive and potentially impacted by subtle chemical changes, two FLIPR® Membrane Potential Assay Kits (Red and Blue) are available to select the optimal conditions for your delicate ion channel targets. (moleculardevices.co.jp)
  • Unlike traditional dyes such as DiBAC, FLIPR Membrane Potential Assay Kit detects bidirectional gradient changes so you can monitor both variable and control conditions within a single experiment. (moleculardevices.co.jp)
  • Comparing data generated using the FLIPR Membrane Potential Assay Kits with results from the manual patch clamp method shows good correlation (Figures 1 and 2). (moleculardevices.co.jp)
  • Because ion channel activity is sensitive to interference, and chemical interference with a particular ion channel is highly unpredictable, the FLIPR Membrane Potential Assay Kits have two formulations. (moleculardevices.co.jp)
  • FLIPR Membrane Potential Assay Kits detect ion channel modulation by increasing or decreasing the fluorescent signal as cellular membrane potential changes. (moleculardevices.co.jp)
  • The kits are uniquely suited for use with the simultaneous pipet and read capability of the FLIPR® Tetra System and FlexStation® 3 Microplate Readers to capture fast kinetics associated with ion channel activation. (moleculardevices.co.jp)
  • The patented quench technology (U.S. patent number 6,420,183, EPO patent number 0 906 572) is offered to drug discovery and life science researchers exclusively by Molecular Devices through the purchase of FLIPR Assay Kits. (moleculardevices.co.jp)
  • FLIPRTETRA is also the first FLIPR® system to offer an expanded set of excitation wavelengths, significantly broadening its use beyond traditional calcium mobilization and membrane potential assays. (moleculardevices.com)
  • With FLIPR Tetra , researchers can now increase the throughput and reduce the cost of their calcium mobilization and membrane potential assays even more-screening over 3,000 kinetic assays every six minutes using a few thousand cells per assay. (moleculardevices.com)
  • Mitochondrial regulation of apoptosis further downstream was investigated, showing changes in the mitochondrial membrane potential, cytochrome c release into the cytoplasm, and enhanced activation of cleaved caspase-3 and Poly ADP-ribose polymerase (PARP). (hindawi.com)
  • Caspase 3/7 and cAMP ranges had been measured utilizing ELISA kits . (aabioetica.org)
  • Annexin V、TUNEL、Mitochondrial Membrane Potential, Caspase and other cell Apoptosis Detection kit and cell senescence staining kit. (abbkine.com)
  • Caspase activity detection kit (apoptosis)-- quick and convenient operation, can read the value only in three steps, and can measure the enzyme activity directly by the standard curve. (abbkine.com)
  • U937 Necroptosis assay U937 cells (2104 cells) were pretreated with compound and pan caspase inhibitor zVAD for 1 h. (lavoixdesrroms.org)
  • The individual synthetic tetrapeptide substrates, together with the caspase inhibitors and active caspase enzymes, offer flexibility in the experimental design of a caspase activity assay. (bdbiosciences.com)
  • This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. (norgenbiotek.com)
  • Suitable assays can be combined and performed sequentially on the same cells in order to reduce assay-specific false positive or false negative results. (wikipedia.org)
  • PCR assay or culture isolation and proved suitable to probe hydrolysis technology (Applied Biosystems, Foster detect SARS-CoV in clinical specimens. (cdc.gov)
  • The Committee noted progress in the development of a solid-membrane assay suitable for use in the field which could detect the presence of orthopoxviruses or varicella virus in individuals presenting with symptoms consistent with smallpox infection. (who.int)
  • Positively charged CTAC could actively target mitochondria by interacting with the negatively charged mitochondria membrane, and then lead to the dysfunction of mitochondria by decreasing mitochondrial potential and intracellular ATP levels, resulting in the necrosis and apoptosis of MCF-7 cells. (rsc.org)
  • Ultimately, these assays enable researchers to obtain a better understanding of the oxidative stress associated events that occur as a result of drug treatments, various disease states, and other experimental conditions. (immunochemistry.com)
  • This study investigated the protective effects of membrane vesicles derived from D. radiodurans (R1-MVs) against H 2 O 2 -induced oxidative stress in HaCaT cells. (biomedcentral.com)
  • Taken together, R1-MVs exert significant protective effects against H 2 O 2 -induced oxidative stress in keratinocytes and have the potential to be applied in radiation-induced oxidative stress models. (biomedcentral.com)
  • Consequently, elamipretide may have a therapeutic potential in preventing damage from the oxidative stress and neuroinflammation that contribute to perioperative neurocognitive disorders (PND), which makes mitochondria a potential target for treatment strategies for PND. (biomedcentral.com)
  • Consequently, alleviating mitochondrial oxidative stress and protecting mitochondrial function provides a potential method for the treatment of these diseases. (biomedcentral.com)
  • From ELISA development and plate coating to conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay. (immunochemistry.com)
  • The degrees of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), superoxide dismutase (SOD), complete antioxidant capability (T-AOC), and glutathione peroxidase (GSH-Px) had been detected by ELISA kits . (aabioetica.org)
  • Samples were analyzed using the A40 Human ELISA Kit (Life Technologies) following the manufacturers instructions. (lavoixdesrroms.org)
  • Les échantillons de sérum de 140 patients susceptibles d'avoir contracté le virus de la dengue ont été analysés entre mai et octobre 2015 à l'aide du test ELISA et de l'amplification en chaîne par polymérase multiplexe. (who.int)
  • mtCheck™ Enhanced Mitochondrial Membrane Potential Assay Kits (JC-1) is a kit that uses JC-1 as a fluorescent probe to quickly and sensitively detect changes in cell, tissue or purified mitochondrial membrane potential. (mitochondriasci.com)
  • The manual patch clamp method is able to detect fast responses allowing detection of very rapid changes in membrane potential. (moleculardevices.co.jp)
  • The assay can detect as low as 1 µM ADP in biological samples. (celltechgen.com)
  • A real-time reverse transcription-polymerase chain (e.g., gel electrophoresis), were time-consuming, and were reaction (RT-PCR) assay was developed to rapidly detect prone to false-positive results from amplicon contamina- the severe acute respiratory syndrome-associated coron- tion. (cdc.gov)
  • However, RIDTs have limited sensitivity to detect influenza virus infection and negative test results should be interpreted with caution given the potential for false negative results. (cdc.gov)
  • Moreover, cell wall damage, cell membrane depolarization, intracellular adenosine triphosphate (ATP) decline, Ca 2+ and Mg 2+ leakage, cell morphological destruction and alterations in intracellular biomolecules were observed, which indicated the negative influence of MDA on cell membrane and cellular homeostasis. (frontiersin.org)
  • Induced pluripotent stem cells are most useful when combined with a high throughput in vitro assay that can recognize hepatotoxicity. (enzolifesciences.com)
  • In vitro apoptosis in individual cells was determined by terminal desoxyriboxyl-desoxyriboxyl transferase-mediated DVTP nick-end labeling (TUNEL) assay with the In Situ Cell Death Assay Kit (Roche Diagnostic Corp., Indianapolis, IN) and visualized by fluorescent microscopy. (cdc.gov)
  • In order to test our hypothesis, we examined, for the first time in vitro , the anti-proliferative properties of the spider-venom peptide gomesin and its analogues as potential therapeutic lead candidates against DFTD. (nature.com)
  • Enzo Life Sciences offers a comprehensive product portfolio for advancing your discoveries in toxicity research with a whole portfolio of toxicity research reagents and kits, including the SCREEN-WELL® Hepatotoxicity library . (enzolifesciences.com)
  • We focus on developing assays that allow researchers to study cellular functions using cell-permeable reagents. (biosciregister.com)
  • 9 Although the design of nanoparticles with triphenylphosphonium cation (TPP, the mitochondrion-targeting moiety) offers potential for mitochondrion-targeting therapeutics, it is still a challenge to actively deliver therapeutic agents to mitochondria due to the selective permeability of the mitochondrial membrane. (rsc.org)
  • If used in combination with high-throughput systems and toxicity assays, high-content imaging has the benefit of being able to screen many compounds at a time to assess multiple parameters including small phenotypic changes. (enzolifesciences.com)
  • The assay is simple, sensitive, stable and high-throughput adaptable. (celltechgen.com)
  • Sunnyvale, Calif., June 1, 2004 - Molecular Devices Corporation (Nasdaq: MDCC), today announced the introduction of FLIPRTETRA the first system that enables researchers to significantly increase throughput of cell-based assays by measuring 1536 kinetic measurements simultaneously. (moleculardevices.com)
  • LDH is a stable cytoplasmic enzyme found in all types of cells and is released into the cell culture medium when the plasma membrane is damaged. (dojindo.com)
  • These include changes in the plasma membrane (such as loss of membrane symmetry and attachment), a condensation of the cytoplasm and nucleus, protein cleavage, and internucleosomal cleavage of DNA. (bdbiosciences.com)
  • Changes in the plasma membrane are one of the characteristics of the apoptotic process detected in living cells. (bdbiosciences.com)
  • Apoptosis can be detected by the presence of phosphatidylserine (PS), which is normally located on the cytoplasmic face of the plasma membrane. (bdbiosciences.com)
  • Since intracellular annexin V is also exposed if the plasma membrane is compromised, a membrane-impermeant dye such as 7-AAD is commonly used to distinguish between apoptotic and dead cells to exclude the dead cells. (bdbiosciences.com)
  • Diagnostic assays. (who.int)
  • Rapid molecular assays are a new type of influenza diagnostic test that use isothermal nucleic acid amplification for viral detection. (cdc.gov)
  • This unique formula responds 10 times faster and has greater temperature stability than traditional dyes, providing high quality screening data that shows good correlation with manual patch clamp assays. (moleculardevices.co.jp)
  • Potentiometric dyes for measuring mitochondrial membrane potential. (stratech.co.uk)
  • The fluorescent signal increases in intensity during membrane depolarization as dye follows the positively charged ions inside the cell. (moleculardevices.co.jp)
  • The abnormal functions of mitochondria in cancer cells result in increased mitochondrial membrane potential (hyperpolarization) and excessive ROS production. (dojindo.com)
  • Cell senescence detection kit (cell senescence) is used for senescence detection of cultured cells and tissue sections. (abbkine.com)
  • This new instrument platform also extends the user base for our growing family of reagent kits, including Calcium 3 and membrane potential kits. (moleculardevices.com)
  • Such ATP-based assays include bioluminescent assays in which ATP is the limiting reagent for the luciferase reaction. (wikipedia.org)
  • only lactate dehydrogenase does not lose its activity during cell death assays. (dojindo.com)
  • One molecule, lactate dehydrogenase (LDH), is commonly measured using LDH assay. (wikipedia.org)
  • Each homogeneous assay kit utilizes a proprietary indicator dye and quencher combination to maximize cell line/channel/compound applicability, while eliminating causes of data variability. (moleculardevices.co.jp)
  • Cell Meter™ JC-10™ assay kits for measuring mitochondrial membrane potential. (stratech.co.uk)
  • In addition, curcumin contributed to cell survival, as indicated by the restoration of the mitochondrial membrane potential (MMP) and the inhibition of the mitochondrial biogenesis induced by HFFA. (spandidos-publications.com)
  • Curcumin also exerts potential anti-inflammatory effects in diverse cell types, including pancreatic cells, chondrocytes, and hepatic cells ( 9 , 10 ). (spandidos-publications.com)
  • Therefore, cell death assays based on LDH activity are more reliable than other enzyme-based cell death assays. (dojindo.com)
  • WST-8, a highly stable WST, is utilized in Cell Counting Kit-8 (CCK-8). (dojindo.com)
  • Dehydrogenase-based assays reflect cell conditions with more sensitivity than the other assays because they depend on several elements including dehydrogenases, NAD(H), NADP(H), and mitochondrial activity. (dojindo.com)
  • The CCK-8 assay involves most of the dehydrogenase in a cell, while MTT only involves mitochondrial dehydrogenase. (dojindo.com)
  • Therefore, the MTT assay depends on mitochondrial activity, not the cell itself. (dojindo.com)
  • Learn about the assays used to assess cell proliferation and the cell cycle. (abcam.com)
  • Induced pluripotent stem cell-derived hepatocytes have a primary tissue-like phenotype, constant and indefinite disposal, and the potential to establish genotype-specific cells from different individuals, which make them an interesting means of predicting drug-induced toxicity. (enzolifesciences.com)
  • Mitochondrion is a common membrane-coated organelle that participates in energy conversion, homeostasis of calcium, cell signaling and cell death. (rsc.org)
  • In addition, gain and loss-of-function studies with miR-206 mimics and inhibitors showed that inhibition of miR-206 in hypoxic MSCs promoted the migration ability of the cells, prevented cell apoptosis, and protected membrane potential of mitochondria, while the benefits were all blocked by Pim-1 inhibitor. (biomedcentral.com)
  • The cells may undergo necrosis, in which they lose membrane integrity and die rapidly as a result of cell lysis. (wikipedia.org)
  • Compounds that have cytotoxic effects often compromise cell membrane integrity. (wikipedia.org)
  • however, if the cell membrane has been compromised, they freely cross the membrane and stain intracellular components. (wikipedia.org)
  • The live-cell protease is only active in cells that have a healthy cell membrane, and loses activity once the cell is compromised and the protease is exposed to the external environment. (wikipedia.org)
  • The dead-cell protease cannot cross the cell membrane, and can only be measured in culture media after cells have lost their membrane integrity. (wikipedia.org)
  • With the addition of these new formats, more complex assays can be developed to look at apoptosis within heterogeneous cell subsets. (bdbiosciences.com)
  • The current examine was undertaken to find out the potential neuroprotective results and the related mechanism of papaverine (PAP), a PDE10A isoenzyme inhibitor, towards quinolinic acid (QUIN)-induced excitotoxicity utilizing human major cortical neurons. (aabioetica.org)
  • Sericin (Ser) is a natural neuroactive macromolecule with diverse pharmacological properties, and our previous findings have shown its neuroprotective potentials. (cjphysiology.org)
  • Both formulations combine the advantages of Molecular Devices proprietary membrane potential indicator dye with our patented quench technology. (moleculardevices.co.jp)
  • Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC. (hindawi.com)
  • Therefore, these miRNAs have potential as new therapeutic biomarkers for BCR-ABL1 status in adult CML patients treated with TKIs at molecular responses. (biomedcentral.com)
  • For this reason, implies a more detailed understanding and description of a risk assessment is required to characterize the potential events, often at the molecular level, than is meant by mode for adverse health effects associated with acute exposures. (cdc.gov)
  • 1 RIDTs do not include rapid molecular assays. (cdc.gov)
  • At present, only one rapid molecular assay is FDA-approved for use in the United States. (cdc.gov)
  • Biodyne B Membrane contains exclusively positive charges, providing the highest possible binding capacity for negatively charged molecules like DNA and RNA. (thermofisher.com)
  • NSCs provide a good platform to screen drugs for neurodegenerative diseases and also have potential applications in regenerative medicine. (koreascience.kr)
  • Luciferase activity assay further confirmed Pim-1 as a putative target of miR-206. (biomedcentral.com)
  • Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. (celltechgen.com)
  • The assay is based on spectrophotometric detection of the chromophore p- nitroaniline (pNA) after cleavage from the labeled substrate WEHD-pNA. (celltechgen.com)
  • Calcein-AM is used for the kit because it is retained inside of cells better than the other fluorescent esterase substrates. (dojindo.com)
  • JC-10™ and JC-1 dual-emission mitochondrial membrane potential probes. (stratech.co.uk)
  • Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. (hindawi.com)
  • It also recommended that validated detection kits should be distributed to designated reference laboratories, and that information on procedures for shipping clinical samples from suspected cases to an appropriate reference laboratory should be widely disseminated. (who.int)
  • Inhalation Toxicology Downloaded from informahealthcare.com by CDC Information Center on 07/06/12 alternative to methyl bromide as a fumigant, there is potential here as a sequence of key events and processes, starting for human exposures to MeI. (cdc.gov)
  • Those who completed the 24-hour urine collection were given a home urine collection (HUC) kit and asked to collect two urine specimens at their home: a void in the evening and the first void of the following morning. (cdc.gov)
  • Specimens included oro- reaction (RT-PCR) assays. (cdc.gov)
  • A variety of RT-PCR assays were developed during the media), sputa, nasal aspirates and washes, bronchoalveo- epidemic for SARS-CoV (1,5-8), including a commercial lar lavage, and lung tissue specimens collected at autopsy. (cdc.gov)
  • Thermo Scientific Biodyne B Nylon Membranes are sheets of positively charged nylon that provide excellent binding characteristics for nucleic acid transfer, blotting and detection methods. (thermofisher.com)
  • You can view and compare kit components in the Components Table in the Product Overview section. (norgenbiotek.com)
  • A widely used assay for staining dead cells is Trypan Blue. (dojindo.com)
  • Among the enzyme-based assays, the MTT assay is the best known for determining mitochondrial dehydrogenase activities in living cells. (dojindo.com)
  • Upon LPS stimulation, the expression of key TCA enzymes, including citrate synthase, α-ketoglutarate dehydrogenase, and isocitrate dehydrogenase 2, and the mitochondrial membrane potential decreased, whereas the levels of LC3b and ROS increased. (nature.com)
  • Conclusions: In conclusion, these findings collectively demonstrate that Nanog, which is highly expressed in CRC stem cells, is a key factor in the development of tumor growth, and it may serve as a potential marker of prognosis and a novel and effective therapeutic target for the treatment of CRC. (researchsquare.com)
  • MMP-3 (stromelysin-1, transin-1) is proposed as a potential therapeutic target. (anaspec.com)
  • Cells undergoing necrosis typically exhibit rapid swelling, lose membrane integrity, shut down metabolism, and release their contents into the environment. (wikipedia.org)
  • They will shut down metabolism, lose membrane integrity and lyse. (wikipedia.org)
  • Alternatively, membrane integrity can be assessed by monitoring the passage of substances that are normally sequestered inside cells to the outside. (wikipedia.org)
  • Camptothecin induced mitochondrial membrane potential changes were measured with JC-10™ (Cat No. 22204 ) and JC-1 (Cat No. 22200 ) in Jurkat cells. (stratech.co.uk)
  • The localization pattern of capsid expression was analyzed by immunofluorescent assay in HeLa, 293-T, RD, or SH-SY5Y cells by using anti-His tag antibody as described ( 6 ). (cdc.gov)
  • The screening of a novel panel of AgGom-analogues revealed that, unlike changes in the hydrophobicity and electrostatic surface, the cytotoxic potential of the gomesin analogues in DFTD cells lies on specific arginine substitutions in the eight and nine positions and alanine replacement in three, five and 12 positions. (nature.com)
  • 4 The mitochondrial membrane potential of cancer cells (∼−220 mV) is more negative than that of normal cells (∼−160 mV). 5 Therefore, mitochondria have been regarded as a novel target for cancer therapy. (rsc.org)
  • Previous studies have shown that it is closely linked with cancer stem cells (CSCs), which have self-renewal potential and the capacity to differentiate into diverse populations. (researchsquare.com)
  • MC65 Neuroprotection assay MC65 cells were washed twice with PBS, resuspended in Opti-MEM, and seeded in 96-well plates (4104 cells/well). (lavoixdesrroms.org)
  • MC65 Mitochondrial membrane potential assay MC65 cells were collected and washed twice with PBS, resuspended in Opti-MEM, seeded in 6-well plates (1.6106 cells/well), and incubated with compounds at 37 C under ?TC conditions for 48 h. (lavoixdesrroms.org)
  • SH-SY5Y Mitochondrial membrane potential assay SH-SY5Y cells (4105 cells) were plated in 12-well plates. (lavoixdesrroms.org)
  • The specimen can be stored at 2-8°C if it is to undergo assay within 5 days following collection. (medscape.com)