Membrane phosphotyrosine binding domain. Medical search. Frequent questions

Following ligand binding of the Notch receptor, the intracellular fragment of Notch (NICD, or notch intracellular domain) is released into the cytoplasm and transported to the nucleus, where it can form a complex with binding partners such as EP300 and histone acetyltransferase and act as a transcription factor for Notch target genes. (wikipedia.org)
The addition of an affinity tag allowed us to avoid the use of antibodies targeted toward the intracellular C-terminal tail of APP, a region to which a number of reported interacting proteins bind ( King and Turner, 2004 ). (jneurosci.org)
Critical for cellular organization, phosphatidylinositol phosphate (PIP) lipids regulate the localization and activity of numerous proteins across intracellular membranes in eukaryotic cells ( Di Paolo and De Camilli 2006 ). (elifesciences.org)
Biochemical data have established the role of the conserved DH domain in Rho GTPase interaction and activation, and the role of the tandem PH domain in intracellular targeting and/or regulation of DH domain function. (embl-heidelberg.de)
The protease not only releases small peptides, such as the amyloid-β peptide, which drives Alzheimer's disease pathogenesis, but also intracellular domains, which can have critical functions in nuclear signaling. (cipsm.de)
Each subunit has a large intracellular loop between the third and fourth transmembrane domains that is the likely site for regulated interactions with postsynaptic scaffolding proteins. (jneurosci.org)
RASER binds to the intracellular part of ErbB, which is the first node in the ErbB signaling pathway. (baybridgebio.com)
When two ErbB molecules are bound together, the intracellular part of one ErbB "activates" the intracellular part of another ErbB. (baybridgebio.com)
In general, Janus family tyrosine kinases (JAKs) bind to the intracellular components of cytokine receptors, and are, in turn, bound by STATs upon cytokine signaling. (medscape.com)

Notch binds the ligands Delta and Serrate in Drosophila. (wikipedia.org)
These ligands are themselves integral membrane proteins. (wikipedia.org)
Interestingly, aux depletion causes a dramatic increase in EGFR signaling, with a strong accumulation of EGFR at the plasma membrane and an increased expression of EGFR ligands in response to tissue stress. (sdbonline.org)
In healthy cells, ErbB1 is only active when it is bound by extracellular ligands. (baybridgebio.com)

AFAP1L1 intersects several invadopodia pathway components through its multiple domains and motifs, including the following (i) pleckstrin homology domains that bind phospholipids generated at the plasma membrane by phosphoinositide 3-kinase, (ii) a direct filamentous-actin binding domain and (iii) phospho-tyrosine motifs (pY136 and pY566) that specifically bind Vav2 and Nck2 SH2 domains, respectively. (nature.com)
These phosphotyrosine motifs are essential for AFAP1L1-mediated cytoskeleton regulation. (nature.com)
It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. (avivasysbio.com)
The CCM2 phosphotyrosine binding (PTB) domain is necessary for a canonical interaction with NPxY motifs within CCM1. (unc.edu)
They are able to bind specific motifs containing a phosphorylated tyrosine residue, propagating the signal downstream by promoting protein-protein interactions and/or modifying enzymatic activities. (eu.org)
Several different binding motifs are known, for example: pYEEI (Src-family SH2 domains), pY [IV]. (eu.org)
The C-terminal half of the SH2 domain exhibits greater structural variability and provides a platform for accommodating different kinds of SH2-binding motifs. (eu.org)
It does not share significant sequence homology with other subtypes of small G-protein GEF motifs such as the Cdc25 domain and the Sec7 domain, which specifically interact with Ras and ARF family small GTPases, respectively, nor with other Rho protein interactive motifs, indicating that the Dbl family proteins are evolutionarily unique. (embl-heidelberg.de)

Receptor dimerization and autophosphorylation attracts proteins containing Src homology 2 (SH2) or phosphotyrosine binding (PTB) domains including adaptor proteins like FRS2 and GRB2. (springer.com)
The VEE is a heterogeneous compartment containing the Adaptor Protein Phosphotyrosine Interacting with Pleckstrin homology Domain and Leucine Zipper 1 (APPL1) with distinct functions in regulating endosomal Gαs/cAMP signaling and rapid recycling. (frontiersin.org)
Pleckstrin homology domain. (embl.de)
Pleckstrin homology (PH) domains are small modular domains that occur in a large variety of proteins. (embl.de)
We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays that utilizes purified Src Homology 2 (SH2) domains as a molecular tool to profile the global tyrosine phosphorylation state of cells. (biomedcentral.com)
An important consequence of reversible phosphorylation of tyrosine residues on proteins is the creation of binding sites for phosphotyrosine recognizing domains such as Src homology 2 (SH2) and phosphotyrosine binding (PTB) domains [ 1 , 2 ]. (biomedcentral.com)
Activated receptor tyrosine kinases bind the Shc adaptor protein through its N-terminal phosphotyrosine-binding (PTB) and C-terminal Src homology 2 (SH2) domains. (elsevierpure.com)
After binding, Shc is phosphorylated within the central collagen-homology (CH) linker region on Tyr-317, a residue remote to both the PTB and SH2 domains. (elsevierpure.com)
Src Homology 2 (SH2) domains are small modular domains found within a great number of proteins involved in different signalling pathways. (eu.org)
The Src Homology 2 (SH2) domain is a major protein interaction module that is central to tyrosine kinase signaling. (eu.org)
The proteins encoded by members of the Dbl family share a common domain, presented in this entry, of about 200 residues (designated the Dbl homology or DH domain) that has been shown to encode a GEF activity specific for a number of Rho family members. (embl-heidelberg.de)
Phosphorylated STATs dimerize within the cytosol via their phosphotyrosines and Src-homology 2 (SH2) domains. (medscape.com)

Another proposed component of the localization complex is Partner of Numb (PON), which is asymmetrically localized during mitosis and acts as an adaptor protein by binding and mediating the anchoring of Numb. (wikipedia.org)
NCK is a major adaptor protein involved in membrane receptor signalling and modulation of actin cytoskeleton dynamics. (eu.org)

Membrane trafficking of G protein-coupled receptors (GPCRs) tightly orchestrates the signaling from this superfamily of signaling receptors. (frontiersin.org)
The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. (avivasysbio.com)
Cell membranes are highly enriched in signaling receptors, transmembrane mechanosensors, pumps and channels, and, depending on their makeup, can recruit and retain a pool of mechanosensors important in the field of mechanobiology. (mechanobio.info)
Dramatic changes in domain coupling and structural rigidity, induced by Tyr-317 phosphorylation, may alter Shc function, bringing about marked differences in the association of unphosphorylated and phosphorylated Shc with its numerous partners, including activated membrane receptors. (elsevierpure.com)
The class 1A phosphoinositide 3-kinase (PI3K) beta (PI3Kβ) is functionally unique in the ability to integrate signals derived from receptor tyrosine kinases (RTKs), heterotrimeric guanine nucleotide-binding protein (G-protein)-coupled receptors (GPCRs), and Rho-family GTPases. (elifesciences.org)
To decipher how amplification of PI(3,4,5)P 3 arises from the relay of signals between cell surface receptors, lipids, and peripheral membrane proteins, we must understand how membrane localization and activity of PI3Ks is regulated by different signaling inputs. (elifesciences.org)
Rather all observations of receptors and factors are the reliable tyrosines but at best the kinase can initiate the loop and synthesis of the ricin, be the gene or significance on Text-to-Speech to bind physiological site. (erik-mill.de)
If there encodes a generation between active cardiomyocytes or cells also this investment can make maintain the most catalytic membrane for receptors with family. (erik-mill.de)
ErbB, like most cell surface receptors, is a transmembrane protein - part of the protein extends out from the cell membrane and interacts with the extracellular environment, and part of the protein lies inside the cell (in the "cytoplasm") and sends signals within the cell. (baybridgebio.com)

The Tir protein of EPEC binds NCK1/NCK2 SH2 domains through a high affinity pYDEV motif ( Frese,2006 ). (eu.org)

Son of sevenless (SOS) is then recruited to the plasma membrane and catalyzes the conversion of inactive Ras-GDP to active Ras-GTP that in turn recruits Raf to the plasma membrane. (springer.com)

Fluorescence microimaging cellular uptake studies with fluorescein-attached LPA4 (F-LPA4) alone or with the mixture of LPA4 and F-GpYEEI in BT-20 cells showed dramatic increase of the fluorescence intensity in cytosol of cells, indicating that LPA4 can function as a delivery tool of F-GpYEEI across the cell membrane. (omicsdi.org)

The specific activity of the PI 3-kinase immunoprecipitated with anti-phosphotyrosine antibody was 3-fold higher than that with anti-PI 3-kinase antibody. (edrc2013.org)

It states that a complex phosphorylation cascade enables aPKC to phosphorylate Numb in the pre-mitotic cell, decreasing its affinity for the plasma membrane. (wikipedia.org)
Through a novel CCM2 PTB domain - Smurf1 HECT domain interaction, CCM2 recruits Smurf1 to specific locations at the plasma membrane where it specifically degrades RhoA. (unc.edu)
The GFRα family consists of four members, GFRα1, GFRα2, GFRα3 and GFRα4, located roughly extracellular and anchored to the plasma membrane by glycosyl-phosphatidyl-inositol (GPI). (medsci.org)
The GFRα family consists of four members, GFRα1, GFRα2, GFRα3 and GFRα4, which are tethered to the plasma membrane through GPI anchors containing CRDs. (medsci.org)
The particular strengths of the work arise to a great extent from the reconstitution system better mimicking the natural environment of the plasma membrane than previous setups have. (elifesciences.org)
This study provides the first in vivo evidence of trapped SD proteins in clathrin-coated pits at the plasma membrane when this pathway is disrupted. (sdbonline.org)

Point mutations cluster into the positively charged end of the molecule around the predicted binding site for phosphatidylinositol lipids. (embl.de)

From studies of APP trafficking and metabolism, the following pathways have emerged: in the first, a fraction of APP molecules residing on the cell surface are processed by ADAM/TACE "sheddases" N-terminal to the ectodomain-transmembrane domain to generate an 83 aa membrane-tethered stub, termed α-CTF. (jneurosci.org)
Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways. (embl.de)
There is a great interest in studying phosphotyrosine dependent protein-protein interactions in tyrosine kinase pathways that play a critical role in many aspects of cellular function. (biomedcentral.com)
[7] [8] Upon binding of type I interferons, IFNAR activates the JAK-STAT signalling pathway , along with MAPK , PI3K , and Akt signaling pathways. (wikidoc.org)
LPL Financial was its Many download Common knowledge tight fluid, which is endophilin Patients that bind aggregates into available development to be the type and an fatty 750 pathways. (erik-mill.de)
KRAS functions as a molecular switch in growth factor pathways, regulating proliferation by alternating between its active (GTP-bound) vs inactive (GDP-bound) state. (cytoskeleton.com)

There are no totally invariant residues within the PH domain. (embl.de)
Isoform gamma contains two PH domains, the second one is split into two parts separated by about 400 residues. (embl.de)
Different families of SH2 domains may have different binding specificity, which is usually determined by a few residues C-terminal with respect to the pY (positions +1 to +4). (eu.org)
The residue at pY+2 does not make direct side chain interactions with the SH2 domain, but aromatic residues are not allowed. (eu.org)
Positively charged residues are disfavoured at pY-1 and pY-2 due to the positively charged SH2 domain surface, but are tolerated when pY+1 and pY+3 are strong residues. (eu.org)
[8] Mutagenesis studies of type I IFNs, IFNAR1 and IFNAR2 demonstrated important binding residues, i.e. "hotspots", on the type I IFN subtypes which influenced its ability to bind to IFNAR2. (wikidoc.org)
residues bind Unsynapsed subsequent download Common knowledge co-transports, bind activated to epithelial cells, and express studied the noise to also also act for themselves, but be as a adenyl signaling the mitochondrial mouse. (erik-mill.de)
Notch Co-Activator and Co-Repressor isoforms: This download Common alters annotated by at least one traditional -domain residues( Co-R) produced to CSL in the water of Notch box-containing. (erik-mill.de)
The side chains of the two histidine and one tyrosine residues that play a role in metal coordination are shown in the Cu(I) bound, Cu(II) bound, and unbound conformations, which differ by only small changes in orientation. (handwiki.org)

Val and Pro confer high-affinity binding at pY+3 - Ala and Ile are tolerated but confer weaker binding. (eu.org)
The binding affinity of an SH2 domain to a pTyr containing ligand is moderate, with the typical affinity range between 0.1 µМ to 10 µМ for equilibrium dissociation constant values (Kd) ( Kaneko,2012 ). (eu.org)
[7] Each type I IFN ligand contains a "hotspot", or a sequence of conserved amino acids that are involved in binding to the receptor, specifically the high affinity receptor IFNAR2, which determines the affinity of each ligand for the receptor. (wikidoc.org)
Arg/Abl2 modulates the affinity and stoichiometry of binding of cortactin to F-actin. (nih.gov)

As the main component, extracellular structure contains some cysteine-rich repeats domains marked as D1-D2-D3 in GFRα1-3, and D2-D3 in GFRα4 ( Figure 1 a ). (medsci.org)
The extracellular E2 domain, a dimeric coiled coil and one of the most highly conserved regions of the protein from Drosophila to humans. (handwiki.org)

This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. (antibodypedia.com)

AD is pathologically characterized by the deposition of pathogenic Aβ peptides that are derived from larger integral membrane proteins, termed β-amyloid precursor proteins (APPs). (jneurosci.org)
Non-phosphorylated peptides do not bind to the SH2 domains. (eu.org)
Although pY peptides strongly localize PI3Kβ to membranes, they only modestly stimulate lipid kinase activity. (elifesciences.org)

The chromo-domain binds histone H3 tails methylated on lysine 9. (cipsm.de)

The results reveal larger phosphotyrosine-binding domain fluctuations and more structural flexibility of unphosphorylated Shc compared with phosphorylated Shc. (elsevierpure.com)
Structural analysis of type I IFN receptor with different type I IFN ligand subtypes revealed a similar binding site for the different agonists. (wikidoc.org)
The PH domain is invariably located immediately C-terminal to the DH domain and this invariant topography suggests a functional interdependence between these two structural modules. (embl-heidelberg.de)
ARS-1620 showed benefits in vivo, and structural studies confirmed that the molecule covalently binds to Cys-12 while the quinazolone core of the molecule occupied the new binding pocket beneath the switch II loop. (cytoskeleton.com)
A number of different structural domains that fold mostly on their own have been found in the APP sequence. (handwiki.org)

SH2 binding kinetics determined by PLA-SH2 showed good agreement with established far-Western analyses for A431 and Cos1 cells stimulated with EGF at various times and doses. (biomedcentral.com)

Each subunit of IFNAR contains an N-terminal ligand binding domain (with two or four fibronectin type II-like subdomains, for IFNAR2 and IFNAR1, respectively), a transmembrane (TM) domain, and a cytoplasmic domain. (wikidoc.org)

Regulation of MT dynamics via direct binding of an Abl family kinase. (nih.gov)

Dimerized STATs then translocate to the nucleus, where they bind DNA in the promoter sequences of target genes to activate transcription. (medscape.com)

Three loops surround the peptide binding pocket and are important for specificity: Because these loops can be flexible, considerable variation in peptide binding can apply for any given SH2 domain. (eu.org)
Tripodal peptide analogues were designed on the basis of the phosphotyrosine binding pocket of the Src SH2 domain and assayed for their ability to bind to fluorescein-labeled phosphopeptides. (omicsdi.org)
Fluorescence polarization assays showed that a number of amphipathic linear peptide analogues (LPAs), such as LPA4, bind to fluorescein-labeled GpYEEI (F-GpYEEI). (omicsdi.org)

Mutations in Brutons tyrosine kinase (Btk) within its PH domain cause X-linked agammaglobulinaemia (XLA) in patients. (embl.de)

The Rho family of GTP-binding proteins has been implicated in the regulation of various cellular functions including actin cytoskeleton-dependent morphological change. (embl-heidelberg.de)

The mechanism by which PI3Kβ prioritizes interactions with various membrane tethered signaling inputs, however, remains unclear. (elifesciences.org)

The mechanism by which MuSK signaling regulates the interaction of the AChR with scaffolding proteins that localize it in the postsynaptic membrane, however, remain unknown. (jneurosci.org)

Regulators of small G-proteins like guanine nucleotide releasing factor GNRP (Ras-GRF) (which contains 2 PH domains), guanine nucleotide exchange proteins like vav, dbl, SoS and Saccharomyces cerevisiae CDC24, GTPase activating proteins like rasGAP and BEM2/IPL2, and the human break point cluster protein bcr. (embl.de)
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. (embl-heidelberg.de)
Activation of Rho proteins through release of bound GDP and subsequent binding of GTP, is catalysed by guanine nucleotide exchange factors (GEFs) in the Dbl family. (embl-heidelberg.de)

Mouse protein citron, a putative rho/rac effector that binds to the GTP-bound forms of rho and rac. (embl.de)
This binding pocket is beneath the effector binding switch-II region and results in disruption of the switch regions leading to a preference for GDP versus GTP. (cytoskeleton.com)

However, it remains unclear where these proteins bind on the AChR and how the interactions are regulated. (jneurosci.org)

The interferon-α/β receptor (IFNAR) is a virtually ubiquitous membrane receptor which binds endogenous type I interferon (IFN) cytokines. (wikidoc.org)
When cytokines bind to their cognate receptor, JAKs phosphorylate the cytokine receptor and subsequently the STATs, which then dissociate from the JAK-receptor complex. (medscape.com)

Aβ is liberated from type I integral membrane proteins, termed β-amyloid precursor proteins (APPs), by the concerted action of β-secretase (BACE1) and γ-secretase (for review, see Selkoe, 2002 ). (jneurosci.org)
Amyloid-beta precursor protein ( APP ) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons . (handwiki.org)

Domain commonly found in eukaryotic signalling proteins. (embl.de)

When GFLs bind with GFRα, they form complexes and associate with the RET receptor, subsequently activating downstream signaling. (medsci.org)
In the presence of either pY/GβGγ or pY/Rac1(GTP), PI3Kβ activity is dramatically enhanced beyond what can be explained by the increase in membrane avidity for these complexes. (elifesciences.org)

At the neuromuscular junction, the acetylcholine receptor (AChR) is specifically clustered in the postsynaptic membrane via interactions with rapsyn and other scaffolding proteins. (jneurosci.org)

[8] The evolutionary conservation of type I IFN subtypes binding the same IFNAR receptor at the same site with differing affinities suggests that type I IFNs are nonredundant and potentially regulate different cellular responses. (wikidoc.org)

If the GST-SH2 and EGFR are in close proximity as a result of SH2-phosphotyrosine interactions, the two oligonucleotides are brought within a suitable distance for ligation to occur, allowing for efficient complex amplification via real-time PCR. (biomedcentral.com)
We showed for the first time that interactions between SH2 domain probes and EGFR in cell lysate can be determined in a microliter-scale assay using SH2-PLA. (biomedcentral.com)
This feature along with short assay runtime makes this method a useful platform for the development of high throughput assays to determine modular domain-ligand interactions which could have wide-ranging applications in both basic and translational cancer research. (biomedcentral.com)
SH2 profiling is a unique proteomic method in which interactions between an array of SH2 domains and protein samples are quantitatively analyzed, thereby defining the functional output of tyrosine phosphorylation. (biomedcentral.com)
Shc phosphorylation plays a pivotal role in the initiation of mitogenic signaling through the Ras/Raf/MEK/ERK pathway, but it is unclear if Tyr-317 phosphorylation affects Shc-receptor interactions through the PTB and SH2 domains. (elsevierpure.com)
In NCK SH2 domains, the EF loop is positioned away from the BG loop, exposing the pY+3 binding pocket where the side chain of Val forms tight interactions. (eu.org)
SH2 domains are phosphotyrosine recognition domains, often mediating transient interactions with target proteins. (eu.org)
Previous experiments have not been able to elucidate whether interactions with membrane-tethered proteins primarily control PI3Kβ localization versus directly modulate lipid kinase activity. (elifesciences.org)
To address this gap in our understanding of PI3Kβ regulation, we established an assay to directly visualize and decipher how three binding interactions regulate PI3Kβ when presented to the kinase in a biologically relevant configuration on supported lipid bilayers. (elifesciences.org)

The labeled SH2 domain probes are incubated with multiple sample spots in a noncompetitive manner (single SH2 per well). (biomedcentral.com)

Here, we define a phosphorylation-dependent binding site on the receptor that mediates agrin-induced clustering. (jneurosci.org)
Phosphorylation of the AChR β subunit is correlated with increased rapsyn/AChR binding, suggesting that the effect of βY390 phosphorylation on clustering is mediated by rapsyn. (jneurosci.org)
Indeed, we found that rapsyn associated with CD4-β loop chimeras in a phosphorylation-dependent manner, and that agrin increased the ratio of rapsyn binding to wild type AChR but not to AChR-β 3F/3F , which lacks β loop tyrosine phosphorylation sites. (jneurosci.org)
Together, these findings suggest that agrin-induced phosphorylation of the β subunit motif increases the stoichiometry of rapsyn binding to the AChR, thereby helping to stably cluster the receptor and anchor it at high density in the postsynaptic membrane. (jneurosci.org)

Using single molecule Total Internal Reflection Fluorescence (TIRF) Microscopy, we determined the mechanism controlling membrane localization of PI3Kβ, prioritization of signaling inputs, and lipid kinase activation. (elifesciences.org)

PEpYLGLD is a sequence that mimics the pTyr1246 of ErbB2 that is responsible for binding to the Chk SH2 domain. (omicsdi.org)
Spa gene: s - signal sequence, E, D, A, B, C sequences encoding IgG-binding domains, X - region which lacks IgG-binding activity and consists of repetitive region (Xr) and C-terminal region (Xc). (cftrpathway.com)

Cytoskeletal proteins such as dynamin (see IPR001401 ), Caenorhabditis elegans kinesin-like protein unc-104 (see IPR001752 ), spectrin beta-chain, syntrophin (2 PH domains) and S. cerevisiae nuclear migration protein NUM1. (embl.de)
This domain, which resembles the structure of spectrin, is thought to bind heparan sulfate proteoglycans. (handwiki.org)

The phosphate group of Tyr is tightly bound in the conserved pTyr binding pocket. (eu.org)
E1 contains two subdomains including a growth factor-like domain (GFLD) and a copper -binding domain (CuBD) interacting tightly together. (handwiki.org)

After binding its hormone FSH, FSHR undergoes internalization to very early endosomes (VEEs) for its acute signaling and sorting to a rapid recycling pathway. (frontiersin.org)

Mutations identified in many SH2 domain-containing proteins as well as the SH2 domain itself are associated with human diseases ranging from cancers, diabetes, to immunodeficiencies. (eu.org)
Mutations to codon 12 in KRAS maintains kRAS in an active, GTP-bound state through preventing GTPase-activating proteins (GAP) association. (cytoskeleton.com)

In the canonical mode of SH2 binding, regions on either side of the central β sheet are involved in ligand binding. (eu.org)

The human NCK1 (Nckα) and NCK2 (Nckβ/GRB4) SH2 domains show a degree of partner specificity but share the same mode of ligand binding ( Frese,2006 ) and belong to the class IA family which contains an aromatic residue (Phe) at the specificity-determining βD5 position ( Kaneko,2010 ). (eu.org)

Several S. cerevisiae proteins involved in cell cycle regulation and bud formation like BEM2, BEM3, BUD4 and the BEM1-binding proteins BOI2 (BEB1) and BOI1 (BOB1). (embl.de)
However, in order to use this method to investigate SH2 binding sites on a specific target in cell lysate, additional procedures such as pull-down or immunoprecipitation which consume large amounts of sample are required. (biomedcentral.com)
These results suggest that PI 3-kinase in signet ring cell carcinoma is recruited to the membrane and activated by the binding to the 200-kDa protein. (edrc2013.org)
The many processes involving SH2 domains range from mitogenic signaling to T cell activation. (eu.org)

The molecular mechanisms underlying this relationship appear to rely on the ubiquitination of the membrane bound Notch1 receptor and the subsequent degradation of its NICD following receptor activation. (wikipedia.org)

Ligand binding to either subunit is required for and precedes dimerization and activation of the receptor. (wikidoc.org)

ITC binding studies confirmed the interaction between [WR]4 and PEpYLGLD. (omicsdi.org)

[20] A serine protease inhibitor domain, absent from the isoform differentially expressed in the brain, is found between acidic region and E2 domain. (handwiki.org)

The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. (wikipedia.org)
The interaction between SH2 domains and their substrates is however dependent also on cooperative contacts of other surface regions. (eu.org)
In 2010 IFLA displayed the naive( regulated) tissue of the relationships for binding units( sodium-dependent university, 120). (erik-mill.de)

The obvious benefit of this method is that the low sample requirement allows detection of SH2 binding in samples which are difficult to analyze using traditional protein interaction assays. (biomedcentral.com)
One limitation of current SH2 profiling methods is that these assays themselves do not provide the identity of the binding proteins. (biomedcentral.com)

One key mechanism dictating the signal activity of GPCRs is membrane trafficking. (frontiersin.org)

Anatomy35

Organisms9

Chemicals and Drugs110

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes65

Technology, Industry, Agriculture5

Information Science4

Health Care1

Intracellular9

Ligands4

Motifs8

Homology12

Adaptor protein2

Receptors9

Nck2 SH21

Recruited to the plasma membrane1

Cytosol1

Antibody1

Plasma6

Phosphatidylinositol1

Pathways6

Residues9

Affinity4

Extracellular2

Endosomal1

Peptides3

Lysine1

Structural5

Kinetics1

Transmembrane1

Dynamics1

Nucleus1

Peptide3

Tyrosine kinase1

Actin cytoskeleton1

Tethered signali1

Localize1

Guanine nucleotide exchange3

Effector2

Proteins bind1

Cytokines2

Integral2

Eukaryotic1

Complexes2

Acetylcholine receptor1

Cellular1

Interactions9

Probes1

Phosphorylation4

Localization1

Sequence2

Spectrin2

Tightly2

Pathway1

Mutations2

Canonical1

Specificity1

Cell4

Subsequent1

Subunit1

Studies confirmed1

Serine1

Dependent3

Assays2

Mechanism1