• P-selectin, a granule membrane protein of platelets and endothelial cells, follows the regulated secretory pathway in AtT-20 cells. (rupress.org)
  • To investigate whether the mechanism of targeting of P-selectin to granules is specific for megakaryocytes and endothelial cells and/or dependent on von Willebrand factor, a soluble adhesive protein that is stored in the same granules, we have expressed the cDNA for P-selectin in AtT-20 cells. (rupress.org)
  • In a study of glial tumors, the cell membranes of glial and endothelial cells were found to contain LAMP1 and LAMP2, while YKL-40 (a different glycoprotein) was found in the cytoplasm. (wikipedia.org)
  • E-Selectin, also known as CD62E, is expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. (rndsystems.com)
  • P-selectin, soluble endothelial protein C receptor, soluble thrombomodulin, plasminogen activator inhibitor 1, ADAMTS-13, von Willebrand factor, tissue factor, soluble intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 were more elevated in LF patients than in controls. (cdc.gov)
  • P-Selectin is stored in the α-granules of platelets and the Weibel-Palade bodies of endothelial cells, and is rapidly transported to the plasma membrane upon activation. (bdbiosciences.com)
  • Endothelial cells in tumor deposits of patients receiving ipilimumab plus bevacizumab were also associated with increased expression of E-selectin, and adhesion and diapedesis of CD8+ T cells. (creativebiolabs.net)
  • This Mouse Endothelial Leukocyte Adhesion Molecule 1 (E-Selectin) ELISA Kit from Innovative Research is intended for quantitative detection of mouse soluble E-Selectin in cell culture supernates, serum and plasma (heparin, citrate). (innov-research.com)
  • Expression system for standard: E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. (innov-research.com)
  • A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. (innov-research.com)
  • Diabetic nephropathy (DN) is a leading selectin), and soluble thrombomodulin--are cause of chronic renal failure and is a grow- providing further evidence of the relation- ing concern given the increasing incidence ship between endothelial cell activation and of type 2 diabetes. (who.int)
  • However, the biomarkers sICAM-1 and sE-selectin (as mechanism of this immune dysregulation markers of endothelial dysfunction) in dia- is still unclear. (who.int)
  • 1 , 2 P- and E-selectin, expressed on activated platelets and/or endothelial cells, bind to ligands on leukocytes. (ashpublications.org)
  • L-selectin, expressed on leukocytes, binds to ligands on some endothelial cells and on other leukocytes. (ashpublications.org)
  • Exposure of endothelial cells to anti-b2GPI antibodies and their corresponding peptides leads to the inhibition of endothelial cell activation, as shown by decreased expression of the adhesion molecules E-selectin, intercellular adhesion molecule, and vascular cell adhesion molecule and of monocyte adhesion. (medscape.com)
  • P-selectin (PADGEM, GMP-140, CD62) is a transmembrane protein specific to alpha granules of platelets and Weibel-Palade bodies of endotheial cells. (rupress.org)
  • In conclusion, we provide evidence for the regulated secretion of a transmembrane protein (P-selectin) in a heterologous cell line, which indicates that P-selectin contains an independent sorting signal directing it to storage granules. (rupress.org)
  • Lysosome-associated membrane protein 2 (LAMP2), also known as CD107b (Cluster of Differentiation 107b) and Mac-3, is a human gene. (wikipedia.org)
  • Its protein, LAMP2, is one of the lysosome-associated membrane glycoproteins. (wikipedia.org)
  • The protein encoded by this gene is a member of a family of membrane glycoproteins. (wikipedia.org)
  • Online Mendelian Inheritance in Man (OMIM): Lysosome-associated membrane protein 2 - 309060 Konecki DS, Foetisch K, Zimmer KP, Schlotter M, Lichter-Konecki U (October 1995). (wikipedia.org)
  • CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. (biolegend.com)
  • Platelets were analyzed by flow cytometry for relative size, shape, aggregation, fibrinogen binding, P-selectin, and lysosomal-associated membrane protein-1 (LAMP-1) expression. (cdc.gov)
  • CD62P is a 140 kDa type I transmembrane glycoprotein that is also known as P-Selectin, Platelet activation-dependent granule membrane protein (PADGEM), or Granule membrane protein-140 (GMP-140). (bdbiosciences.com)
  • Single-pass type I membrane protein. (qedbio.com)
  • Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:12441351). (qedbio.com)
  • TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, L-selectin, and alpha-cleavage of amyloid protein precursor (APP). (qedbio.com)
  • LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. (exbio.cz)
  • The Golgi apparatus protein 1 (GLG1), which is located in Golgi cisterns of various cell types, can bind fibroblast growth factor and E-selectin. (embl.de)
  • Hence this protein can be used as a sensitive probe for PS exposure upon the cell membrane. (scienceopen.com)
  • Dense granule exocytosis is typically evaluated by ADP/ATP release by using luciferase-based luminescence techniques, release of preloaded [ 3H] serotonin, or membrane expression of lysosome-associated membrane protein 2 (LAMP2) or CD63 by flow cytometry. (cloudfront.net)
  • This glycoprotein provides selectins with carbohydrate ligands. (wikipedia.org)
  • It is believed that adhesive interactions between selectins on the blood vessel wall and selectin ligands on the CTC surface facilitate metastatic progression, in a manner similar to the leukocyte adhesion cascade essential in the inflammatory response (3). (aiche.org)
  • Murine leukocytes are thought to express α2-3-sialylated and α1-3-fucosylated selectin ligands such as sialyl Lewis x (sLe x ), although monoclonal antibodies (mAbs) to sLe x or Le x reportedly do not bind to murine leukocytes. (ashpublications.org)
  • 2H5 and KM93 bound to pronase-resistant structures, indicating that the mAbs did not identify selectin ligands. (ashpublications.org)
  • α-Granule exocytosis is evaluated primarily by plasma membrane expression of P-selectin (CD62P) by flow cytometry or estimation of the release of PF4, VWF, or other granule cargos. (cloudfront.net)
  • And the internal surface has actin filaments such as talin, paxillin, vinculin, tensin and proteins that provide interactions between membrane receptor proteins [ 1 - 3 ]. (omicsonline.org)
  • Proteins which are found in membranes including cellular and intracellular membranes. (curehunter.com)
  • Cell adhesion relies on transmembrane proteins, known as adhesion proteins , found in the plasma membrane. (uvigo.es)
  • They contain mainly proteins, both membrane-associated receptors (for example, αIIbβ3 and P-selectin) and soluble cargo (for example, platelet factor 4 [PF4] and fibrinogen). (cloudfront.net)
  • Upon stimulation of these cells, P-selectin is translocated to the plasma membrane where it functions as a receptor for monocytes and neutrophils. (rupress.org)
  • CD62P antibody LS-B3656 is an unconjugated mouse monoclonal antibody to human CD62P (SELP / P-Selectin). (lsbio.com)
  • Recognizes the CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. (lsbio.com)
  • Immunoelectron microscopy using a polyclonal anti-P-selectin antibody demonstrated immunogold localization in dense granules, morphologically indistinguishable from the ACTH granules. (rupress.org)
  • Binding experiments with radiolabeled monoclonal antibody to P-selectin indicated that there was also surface expression of P-selectin on the AtT-20 cells. (rupress.org)
  • Here, we show that L-selectin-dependent rolling after P-selectin blockade is completely absent in P-selectin glycoprotein ligand-1 (PSGL-1) −/− mice or wild-type mice treated with a PSGL-1 blocking monoclonal antibody. (rupress.org)
  • A monoclonal antibody from rat specific for E-Selectin has been precoated onto 96-well plates. (innov-research.com)
  • Standards(NSO, W22-P557) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for E-Selectin is added subsequently and then followed by washing with PBS or TBS buffer. (innov-research.com)
  • Leukocyte rolling in postcapillary venules of inflamed tissues is reduced in L-selectin-deficient mice and mice treated with L-selectin blocking antibodies, but the glycoprotein ligand for L-selectin in inflamed venules is unknown. (rupress.org)
  • The strength of the adhesion of a cell to the extracellular matrix depends on the number, the active state and type of integrins that are expressed in the plasma membrane. (uvigo.es)
  • CAMs have been classified into: cadherins, the immunoglobulin superfamily, integrins and selectins. (bvsalud.org)
  • Here, we describe a novel approach to functionalize human and murine leukocytes with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on their surface, along with E-selectin (ES), to capture and induce cancer cell apoptosis both in vitro in human blood and in vivo in mouse circulation. (aiche.org)
  • PSGL, P-selectin glycoprotein ligand-1. (rupress.org)
  • Tethered neutrophils enter in membrane-bound vesicles before penetrating into the megakaryocyte cytoplasm. (elifesciences.org)
  • Intracytoplasmic neutrophils develop membrane contiguity with the demarcation membrane system, thereby transferring membrane to the megakaryocyte and to daughter platelets. (elifesciences.org)
  • These findings identify emperipolesis as a new cell-in-cell interaction that enables neutrophils and potentially other cells passing through the megakaryocyte cytoplasm to modulate the production and membrane content of platelets. (elifesciences.org)
  • One of the more common cell traffickers is P-selectin, a molecule that lightly binds the white blood cells called neutrophils. (mit.edu)
  • P-Selectin is thought to mediate the initial adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses, and of activated platelets to neutrophils and monocytes in hemostasis. (bdbiosciences.com)
  • E-Selectin/CD62E " has 39 results in Products. (rndsystems.com)
  • Simple Plex Human E-Selectin/CD62E assay kit for use on Ella instrument. (rndsystems.com)
  • Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT) (PubMed:24226769). (qedbio.com)
  • Extracellular vesicles (EV) has been known as membrane bound vesicles secreted by nearly all cell types. (molcells.org)
  • Upon prolonged high intracellular rises in Ca 2+ , platelets develop a procoagulant phenotype that is characterized by the surface membrane exposure of phosphatidylserine and by the inactivation of integrin α IIb β 3 (procoagulant platelet population) ( 5 ). (frontiersin.org)
  • An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. (innov-research.com)
  • E-selectin was chosen as a target molecule based on its disease-specific expression on activated glomerular endothelium in a mouse anti-glomerular basement membrane glomerulonephritis. (rug.nl)
  • IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. (exbio.cz)
  • By double-label immunofluorescence, P-selectin was visible as a punctate pattern at the tips of cell processes. (rupress.org)
  • Focal attachments have 10-15 nm distance between the cell membrane and the material surface and they are tight connections. (omicsonline.org)
  • Microparticles are plasma membrane vesicle fragments (between 0.1 and 1 μm in size) released from various cell types during activation by agonists or physical or chemical stress, including apoptosis [ 6 , 7 ]. (ersjournals.com)
  • It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. (exbio.cz)
  • Millan J, Cerny J, Horejsi V, Alonso MA: CD4 segregates into specific detergent-resistant T-cell membrane microdomains. (exbio.cz)
  • One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner side of the plasma membrane to the outer layer, by which PS becomes exposed at the external surface of the cell. (scienceopen.com)
  • The difference between these two forms of cell death is that during the initial stages of apoptosis the cell membrane remains intact, while at the very moment that necrosis occurs the cell membrane looses its integrity and becomes leaky. (scienceopen.com)
  • Therefore the measurement of Annexin V binding to the cell surface as indicative for apoptosis has to be performed in conjunction with a dye exclusion test to establish integrity of the cell membrane. (scienceopen.com)
  • 3. Cell membrane. (uvigo.es)
  • Recent reports have shown that an artificially created alkaline environment can influence cell membrane permeability and cell behavior. (oatext.com)
  • The ability of alkaline buffered creatine to produce micro-environmental remodeling, influence cell membrane behavior and impact cisplatin-mediated toxicity, are also examined in this paper. (oatext.com)
  • Hemolytic activity was also assayed to control possibility of direct cell membrane damage [11, 12]. (romj.org)
  • Analysis of blood flow dynamics revealed that discoid platelets preferentially adhere in low-shear zones at the downstream face of forming thrombi, with stabilization of aggregates dependent on the dynamic restructuring of membrane tethers. (nature.com)
  • Nevertheless, forced fucosylation of intact cells did not significantly augment their ability to bind to fluid-phase P- or E-selectin or to roll on immobilized P- or E-selectin under flow. (ashpublications.org)
  • LAMP2, along with LAMP1, interact with E-selectin and galectins to mediate the adhesion of some cancer cells to the ECM. (wikipedia.org)
  • In contrast, the surface expression of P-selectin transfected into CHO cells, which do not have a regulated pathway of secretion, did not change with 8-Br-cAMP treatment. (rupress.org)
  • They are rarely found on the plasma membranes of normal cells, and are found more on highly metastatic tumors than on poorly metastatic ones. (wikipedia.org)
  • A dhesion molecules are found in the plasma membrane. (uvigo.es)
  • For example, cells can change the type and amount of adhesion molecules in the plasma membrane by synthesis, degradation, or hidden them temporarily in internal compartments by endocytosis and exocytosis. (uvigo.es)
  • Another mechanism to control the strength and specificity of adhesion is by activating or inactivating the adhesion molecules in the plasma membrane. (uvigo.es)
  • Creatine does not cross the plasma membrane very well. (oatext.com)
  • The β-secretase cuts APP first to generate the N terminus of Aβ, thus producing a membrane bound C-terminal fragment called C99. (jneurosci.org)
  • Biological Function Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form (PubMed:9034191). (qedbio.com)
  • Background TNFalpha is synthesized as a 26kDa type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17kDa mature TNFalpha. (qedbio.com)
  • TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). (qedbio.com)
  • Phosphatidylserine-exposing platelets, usually located around a thrombus, provide a negatively charged membrane surface, which supports coagulation factor binding and the formation of tenase and prothrombinase complexes ( 6 , 7 ). (frontiersin.org)
  • Here, we review current knowledge how proteases act on platelet receptors and the platelet membrane surface. (frontiersin.org)
  • For metastasis to occur, cancer cells detach from the primary tumor, invade through the basement membrane, and intravasate into the peripheral circulation as circulating tumor cells (CTCs) (2). (aiche.org)
  • The classic representation of α-granules as spherical organelles with a peripheral limiting membrane, a dense nucleoid, and progressively lucent peripheral zones on transmission electron microscopy is probably simplistic and may be in part a preparation artifact. (cloudfront.net)
  • ABSTRACT This study assessed changes in serum levels of cytokines IFN and IL-10 (biomarkers of in ammatory changes) and soluble biomarkers sICAM-1 and sE-selectin (biomarkers of endothe- lial dysfunction) in diabetic patients with and without nephropathy. (who.int)
  • Les numérations plaquettaire et leucocytaire ainsi que la concentration en cytokines (IL-2R, IL-8 et TNF-) ont été me- surées. (who.int)
  • This phenomenon occurs in otherwise unmanipulated murine marrow in vivo, resulting in circulating platelets that bear membrane from non-megakaryocytic hematopoietic donors. (elifesciences.org)
  • Figure 5: Stabilized discoid platelet aggregation occurs via restructuring of membrane tethers. (nature.com)
  • E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM). (innov-research.com)
  • Fractionation of the transfected cells resulted in the codistribution of P-selectin and ACTH in cellular compartments of the same density. (rupress.org)
  • To investigate whether leukocyte-expressed PSGL-1 is mediating L-selectin-dependent rolling, we reconstituted lethally irradiated wild-type mice with PSGL-1 −/− bone marrow cells. (rupress.org)