CD63 antigen is a protein that, in humans, is encoded by the CD63 gene. (wikipedia.org)
main text % % \newpage{} \linenumbers \section*{Introduction} %----------------------------------- \label{Introduction} Successful immunotherapy (IT) of IgE-mediated allergic disease is defined clinically as a long-lasting diminution of immediate hypersensitivity, \emph{i.e.}, greater tolerance, that is induced by repeated antigen exposure over a relatively long time period (\emph{e.g.} weeks-to-months). (openwetware.org)
Intracellular2
CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. (wikipedia.org)
C-terminal mutants showed increased surface expression and decreased intracellular localisation relative to CD63Wt. (wikipedia.org)
Platelet1
Rilzabrutinib demonstrated dose-dependent improvement of clinical scores and joint pathology in a rat model of collagen-induced arthritis and demonstrated reductions in autoantibody-mediated FcγR signaling in vitro and in vivo, with blockade of rat Arthus reaction, kidney protection in mouse Ab-induced nephritis, and reduction in platelet loss in mouse immune thrombocytopenia. (aai.org)
Pathway1
The RNA sequencing results indicated that Mrgprb2 could play a pivotal role in HC injection induced anaphylactoid reaction mediated by mTOR/AMPK pathway. (bvsalud.org)
Histamine1
MATERIALS AND METHODS: Mrgprb2 related anaphylactoid reactions induced by HC injection were investigated by histamine/ß-hexosaminidase releasing, mast cell degranulation, and hind paw swelling assays by using a Mrgprb2 knockout mouse model. (bvsalud.org)
Marker2
CD63 is a good marker for flow cytometric quantification of in vitro activated basophils for diagnosis of IgE-mediated allergy. (wikipedia.org)
In cell biology, CD63 is often used as a marker for multivesicular bodies, which in some cells are enriched with CD63, as well as for extracellular vesicles released from either the multivesicular body or the plasma membrane. (wikipedia.org)
Humans1
Angiogenin expression is developmentally regulated in rats and humans [7,8], predominating in the adult liver of both species [7,9]. (angiogenesis-blog.com)
Human5
title{Protein Tyrosine Phosphatase Inhibition Mimics Fc$\varepsilon$RI-induced Anergy and Reveals the Immunomodulatory Role of Hydrogen Peroxide as a Signaling Molecule in Human Basophils\thanksref{grant}} %Really struggling with a title that captures this paper -- probably a bad sign! (openwetware.org)
In addition to potent and selective BTK enzyme and cellular activity, rilzabrutinib inhibited activation and inflammatory activities of B cells and innate cells such as macrophages, basophils, mast cells, and neutrophils, without cell death (in human and rodent assay systems). (aai.org)
AIM OF THE STUDY: We aimed to investigate the role of the mouse Mas-related G-protein coupled receptor B2 (Mrgprb2) (the orthologous gene of human MRGPRX2) in anaphylactoid reactions induced by HC injection. (bvsalud.org)
Given its known biological activities and its observed pattern of expression, these data suggest that, in human placenta, angiogenin has a role not only in angiogenesis but also in vascular and tissue homeostasis, maternal immune tolerance of the foetus, and host defences. (angiogenesis-blog.com)
It was the first angiogenic protein to be isolated from conditioned medium of human tumour cells, being characterised by its capacity to induce neovascularization [3]. (angiogenesis-blog.com)
Cell2
The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. (wikipedia.org)
As pancreatic ribonuclease is unable to induce angiogenesis, this structural similarity has served to study angiogenins structure/function relationships relative to bovine pancreatic ribonuclease (RNase A). An intact catalytic site and cell-binding domain are required for angiogenin to induce neovascularization ([10], for review). (angiogenesis-blog.com)
Role2
Antibody induced internalisation was reduced in C-terminal deletion mutants and abolished in G→A and Y→A mutants, showing the crucial role of these residues in internalisation. (wikipedia.org)
Our work might shed the light on the role of Mas-related G-protein coupled receptor-X2 (MRGPRX2) in modulating drug-induced anaphylactoid reactions. (bvsalud.org)
Results1
Intriguingly, our results showed that Mrgprb2 might involve in Compound 48/80 induced anaphylactoid reactions mediated by Reelin/E-cadherin axis, which suggested different roles of Mrgprb2 in anaphylactoid reactions induced by HC injection and C48/80. (bvsalud.org)
Allergens2
In addition, Dex carry MHC and T-cell costimulatory molecules to present allergens to induce the production of Th2 cytokines in allergic donors, which are important immunostimulatory factors of anaphylactic immune responses ( 17 , 18 ). (spandidos-publications.com)
Cross-linking of IgE molecules on mast cell and basophil cell membranes by latex protein allergens triggers the release of histamine and other mediators of the systemic allergic cascade in sensitized individuals. (medscape.com)
Mast1
CONCLUSION: Based on biomarker analysis, various endotypes were identified, including type I-like (with the involvement of mast cells or basophils), complement, and cytokine (interleukin-6) release-related reactions, with some patients showing mixed reactions. (bvsalud.org)
Extracellular2
In cell biology, CD63 is often used as a marker for multivesicular bodies, which in some cells are enriched with CD63, as well as for extracellular vesicles released from either the multivesicular body or the plasma membrane. (wikipedia.org)
Exosomes are cell-derived, nm-sized extracellular vesicles (EVs) that are formed through the endocytosis and inward budding of the endosomal membrane mediated by extracellular components and cell surface proteins. (spandidos-publications.com)
Vesicles2
CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. (wikipedia.org)
DC-derived exosomes (Dex) are nano-scale lipid-membrane vesicles formed within DCs by the inward budding of the endosomal membrane after DCs receive immune signals ( 12 , 13 ). (spandidos-publications.com)
Proteins3
The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. (wikipedia.org)
The expression of these marker proteins was measured by the binding of specific antibodies to paraformaldehyde-fixed cells and subsequent flow cytometry. (scienceopen.com)
We first investigated whether the changes in CD63 and CD67 expression after stimulation of intact cells were comparable with earlier measurements of neutrophil degranulation, in which the release of soluble marker proteins was measured. (scienceopen.com)
Molecules1
Once activated, DCs increase the expression levels of the MHC peptide complex and costimulatory molecules, allowing them to efficiently activate T cells ( 22 ). (spandidos-publications.com)
Flow1
Acute samples were obtained from 18 cases for the ex vivo assessment of basophil activation by flow cytometry analysis of CD63, CD203, and FcεRI cell membrane expression. (bvsalud.org)
Cells1
Caspase-1 and granzyme A have been shown to cut GSDMB at specific sites to release the N-terminal fragment of the protein (GSDMB-NT) that has the ability to induce pyroptosis in cells, including airway epithelial cells3,4. (authorea.com)
Activation2
The test is commonly designated as basophil activation test (BAT). (wikipedia.org)
Evidence of basophil activation was obtained in 10 patients. (bvsalud.org)
Present1
We have developed an alternative method to study the degranulation in electropermeabilized human neutrophils by measuring the up-regulation of the specific membrane markers CD63 (residing in the azurophilic granules of resting neutrophils) and CD67 (present in specific granules). (scienceopen.com)
Release1
In this study, the relationship between the expression of PMN degranulation markers (CD63 and CD66b) and the release of degranulation products [myeloperoxidase (MPO) and lactoferrin (LF)] was investigated during clinical HD in order to evaluate cell surface markers as a useful index of PMN degranulation. (scienceopen.com)
Cell surface1
Our data suggest that the measurements of cell surface markers may not be a reliable indicator of the degree of HD-induced PMN degranulation. (scienceopen.com)