Membrane glycosylation. Medical search. Frequent questions

N-linked glycosylation can be seen in antibodies, on cell surfaces, and on various proteins throughout the matrix. (wikipedia.org)
N-linked glycosylation is the most prevalent posttranslational modification of plasma membrane and secretory proteins and participates in many important biological roles such as protein folding, intracellular targeting, immune response, cell adhesion, and protease resistance. (jneurosci.org)
Glycosylation, a post-translational modification affects the biophysical, functional and biochemical properties and provides structural complexity for these proteins. (amrita.edu)
It is known that GSLs interact with both intracellular as well as exogenous proteins and are critical for membrane organisation, signalling, and recognition events. (springer.com)
This review focuses on emerging topics encompassing the functional involvement of aquaporin channel proteins (AQPs) and membrane transport systems, also allowing permeation of NO and hydrogen peroxide, a major ROS, in oxidative stress physiology and pathophysiology. (hindawi.com)
This notion has been challenged by the discovery of new membrane transport functions, especially those exerted by aquaporins (AQPs), a family of membrane channel proteins widespread in nature [ 10 , 11 ]. (hindawi.com)
Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. (biorxiv.org)
Furthermore, we demonstrate the ability of cell-free synthesized OSTs to glycosylate multiple target proteins with varying N -glycosylation acceptor sequons. (biorxiv.org)
yet, so we currently work on problems related to protein transport across membranes and post-translational modification of proteins. (caltech.edu)
The first two systems, Sec and Tat, are pathways for proteins to be translocated across membranes. (caltech.edu)
The second is the essential eukaryotic process of protein glycosylation by which sugars are added specifically to proteins. (caltech.edu)
We are also interested in the general problem of the over-expression and synthesis of membrane proteins. (caltech.edu)
Lectin arrays are widely used tools for analyzing glycosylation in proteins and cells. (muni.cz)
Further analysis and glycoprotein staining revealed that in H. pylori , the PA pathway is necessary for the glycosylation of proteins other than flagellins and for the synthesis of additional virulence factors, including LPS and urease. (uwo.ca)
Glycosylation of those proteins was proven to influence their subcellular locations. (frontiersin.org)
Glycosylation is an important post-translational modification of proteins, and glycoproteins are widely involved in various important cellular biological processes and diseases ( 1 ). (frontiersin.org)
I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
I further investigated membrane proteins and the targeting of tail-anchored proteins at Sheffield Hallam University. (brookes.ac.uk)
The Mucin 1 (MUC1) gene encodes a membrane bound glycosylated phosphoprotein, belonging to the family of mucins which are heavily glycosylated proteins. (sigmaaldrich.com)
Pathophysiology is complex, involving glycosylation of proteins, hormonally influenced cytokine release (eg, transforming growth factor-beta), deposition of mesangial matrix, and alteration of glomerular hemodynamics. (msdmanuals.com)
Hyperglycemia causes glycosylation of glomerular proteins, which may be responsible for mesangial cell proliferation and matrix expansion and vascular endothelial damage. (msdmanuals.com)

Na + K + 2Cl - co-transporters (NKCCs) effect the electroneutral movement of Na + -K + and 2Cl - ions across the plasma membrane of vertebrate cells. (elsevierpure.com)
Unlike NKCC1, NKCC2 is minimally hybrid/complex N-glycosylated under basal conditions and yet it is trafficked to the plasma membrane region of hyper-osmotically challenged cells through mechanisms that require minimal complex N-glycosylation or functional Golgi cisternae. (elsevierpure.com)
However, NKCC2 targeted to the plasma membrane region or transient over-expression of NKCC2 failed to rescue NKCC1 in COS7 cells where NKCC1 had been silenced. (elsevierpure.com)
The protein encoded by this gene is an integral plasma membrane protein which promotes cell adhesion. (thermofisher.com)
Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). (rndsystems.com)
ADRM1 is an integral plasma membrane protein which promotes cell adhesion. (neuromics.com)
However, as opposed to DNA vaccines, RNA vaccines only have to cross the plasma membrane, but not the nuclear envelope which may improve the probability of successful in vivo transfection. (techstartups.com)

The encoded protein is thought to undergo O-linked glycosylation. (thermofisher.com)
ADRM1 is assumed to undergo O-linked glycosylation. (neuromics.com)

The human cellular prion protein (PrP C ) is a glycosylphosphatidylinositol (GPI) anchored membrane glycoprotein with two N-glycosylation sites at residues 181 and 197. (mdpi.com)
Addgene: Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles. (addgene.org)

[ 6 ] In addition, infants and children with various forms of congenital glycosylation defects, another potential mechanism for loss of heparin sulfate proteoglycans, also have PLE secondary to increased intestinal permeability. (medscape.com)

The protein from human and rat brains contains three and four extracellular N-glycosylation sites, respectively. (rti.org)

They can be used for glycan profiling of various samples, including purified glycoproteins, cell/tissue lysates, membrane vesicles, complex biological samples, or entire cells. (muni.cz)

The results clearly highlight the influence of the different fatty acids of the Gb 3 sphingolipids on the phase behaviour and the binding properties of Shiga toxin B subunits, even though the membranes were only doped with 5 mol% of the receptor lipid. (springer.com)
Being typically a minor component of the cell membrane, they are of utmost importance for biological functions that rely on lipid-lipid and lipid-protein interactions (Schnaar and Kinoshita 2015 ). (springer.com)
We already found that non-glycosylated AMPA-R is preferentially localized in lipid raft on the cell membrane and this localization has altered channel property, missing desensitization. (nii.ac.jp)
Throughout the years, ROS and NO have been widely considered to enter cells by freely diffusing through the cell membrane lipid bilayer and not via specific transporters or channels. (hindawi.com)
Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, 'Protein glycosylation B' (PglB) from Campylobacter jejuni , as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari , and Desulfovibrio gigas . (biorxiv.org)

These limitations include a lack of available tools for biochemical characterization of enzymes involved in glycosylation. (biorxiv.org)
Characterization of collagen glycosylation and basement membrane proteoglycans were also major areas of research in the Spiro lab. (glycobiology.org)
Mass spectrum-based high-throughput characterization of glycosylation as well as proteomic analysis has been used for years ( 7 - 11 ). (frontiersin.org)

Glycosylation of peptides is a promising strategy for modulating the physicochemical properties of peptide drugs and for improving their absorption through biological membranes. (rsc.org)
The glycosylation of cells reflects the biological species, tissue, and physiological state of the organism. (muni.cz)
These glycoproteins are widely involved in important biological processes, such as antigen presentation, cytokine production and glycosylation progression. (frontiersin.org)
For better insight into the role of glycosylation in biological progress and diseases, glycosylation profiling strategies are necessary for glycobiology studies. (frontiersin.org)

Whereas the trisaccharidic head group of Gb 3 defines the specificity of Shiga toxin binding, the lipophilic part composed of sphingosine and different fatty acids is suggested to determine its localization within membranes impacting membrane organisation and protein binding eventually leading to protein internalisation. (springer.com)
We also revealed that there are raft-positive and -negative synapses in the same neuron, proposing the hypothesis that glycosylation of AMPA―R decides the synaptic localization, resulting in the regulation of synaptic strength in synaptic plasticity. (nii.ac.jp)
Upregulation of this gene, abnormal intracellular localization and varied glycosylation of Muc1 has been associated with carcinomas. (sigmaaldrich.com)

Over the next five years, our goals are to (1) identify novel recycling receptors required at different Golgi compartments and establish a systematic map of the intra-Golgi recycling network, (2) determine how the transmembrane receptors engage with their cargos, and (3) define the novel functions of a disease-associated membrane transporter in solute transport and protein recycling in the Golgi. (cornell.edu)

Alterations in glycosylation are often acquired in cases of cancer and inflammation, which may have important functional consequences. (wikipedia.org)
Daily exposure of the peritoneal membrane to these solutions may cause complications such as peritonitis, fibrosis and inflammation which, in the long term, lead to the failure of the treatment. (amsterdamumc.org)

Aberrant expression and glycosylation of mucins contribute to tumour survival and proliferation in many cancers, which in turn activates numerous signalling pathways such as NF-kB, ERα, HIF, MAPK, p53, c-Src, Wnt and JAK-STAT, etc. (amrita.edu)
Importantly, changes in glycosylation patterns were observed in various tumors, and the aberrant glycosylation is often associated with malignancy potential, tumor immune surveillance, and patients´ prognosis. (muni.cz)

Here, we review current knowledge how proteases act on platelet receptors and the platelet membrane surface. (frontiersin.org)
One possible mechanism to explain this observation is that a pool of pre-assembled AMPA receptors can be moved from an intracellular compartment to the postsynaptic membrane. (jneurosci.org)
Glycosylation of classical death receptors fine-tunes cell death programs. (nature.com)

We have identified one as a membrane-associated isoform of an immunodominant antigen that protects H. pylori from oxidative stress. (uwo.ca)

Although one typically thinks of carbohydrates as associated with cell growth and viability, glycosylation also has an integral role in many processes leading to cell death. (nature.com)

Publications from the Spiro lab include over 130 primary research papers and 32 reviews providing seminal contributions to glycobiology, including synthesis and catabolism of N-glycans, O-glycan, proteoglycans, and collagen glycosylation. (glycobiology.org)

The fabricated lectin arrays will be used to map the glycosylation changes at different stages of cell differentiation and under different physiopathological conditions. (muni.cz)
Mass spectrometry and lectin arrays are common technologies publicly adopted in glycosylation analysis. (frontiersin.org)

Here we examined the role of rhodopsin glycosylation in biosynthesis, trafficking, and retinal degeneration (RD) using transgenic Xenopus laevis expressing glycosylation-defective human rhodopsin mutants. (jneurosci.org)
The major observation considered to be involved in the pathogenesis of retinal lesions is abnormalities in the internal limiting membrane formed by Müller cells, which is corresponding to the glia limitans formed by astrocytes in the brain. (intechopen.com)
Moreover, fukutin may be involved in synaptic functions of retinal neurons through the glycosylation of α-DG. (intechopen.com)

Further studies suggest that rhodopsin requires glycosylation for incorporation into ROS membranes. (jneurosci.org)

The observed glycomic changes point to changes in protein abundance and protein-specific glycosylation, representing candidate functional biomarkers of PD and associated complications. (amsterdamumc.org)
This review focuses on an emerging topic, the functional involvement of AQPs in ROS membrane transport, with specific regard to the movement of hydrogen peroxide and NO into and out of cells, in both health and oxidative stress-induced diseases. (hindawi.com)

Expression of T17M was more toxic than T4K to transgenic photoreceptors, further suggesting that glycosylation at N15 plays a more important physiological role than glycosylation at N2. (jneurosci.org)
Glycogenes and altered glycosylation patterns, including increased sialylation of O-glycans (2), core fucosylation (3), and glycosyltransferase expression levels (4), have been detected in various cancers. (vectorlabs.com)
We anticipate that this broadly applicable production method will advance glycoengineering efforts by enabling preparative expression of membrane-embedded OSTs from all kingdoms of life. (biorxiv.org)

Mucins belong to a heterogeneous group of large O-glycoproteins that can be either secreted or membrane-bound. (amrita.edu)

Both studies suggest that defects in rhodopsin glycosylation are associated with impairment of protein folding or stability. (jneurosci.org)

Although mutations T4K and T4N caused RD, N2S and T4V did not, demonstrating that glycosylation at N2 was not required for photoreceptor viability. (jneurosci.org)

Glycosphingolipids (GSLs) are a subclass of glycolipids that are found in cell membranes of various organisms ranging from bacteria to humans. (springer.com)
Glycosylation allows fibroblast growth factor 23 to move out of the cell and protects the protein from being broken down. (medlineplus.gov)
Once outside the bone cell, fibroblast growth factor 23 must attach (bind) to a receptor protein that spans the membrane of kidney cells. (medlineplus.gov)
I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
Is there a hallmark 'glycosylation signature' that characterizes the initiation, execution and resolution of cell death programs in physiologic and pathologic settings? (nature.com)
The degree of glycosylation varies with cell type. (sigmaaldrich.com)
Instead, they said that the mRNA vaccine is degraded in the cytosol, a part of the cytoplasm which is outside the cell's nucleus and surrounded by the cell membrane. (techstartups.com)

Upon prolonged high intracellular rises in Ca 2+ , platelets develop a procoagulant phenotype that is characterized by the surface membrane exposure of phosphatidylserine and by the inactivation of integrin α IIb β 3 (procoagulant platelet population) ( 5 ). (frontiersin.org)

This study demonstrates the potential of V H H-based CAR T for pancreatic cancer therapy and provides an engineering strategy for developing potent CAR T cells targeting membrane-distal epitopes. (nature.com)
In this work, we isolate a dromedary camel V H H single-domain antibody (also known as nanobody) named D4 and a monoclonal antibody (mAb) named HM2 recognizing membrane-distal and membrane-proximal epitopes of GPC1, respectively. (nature.com)

Large-scale analysis of glycosylation in organisms includes glycomics (glycan profiling) and glycoproteomics (glycosylation site profiling) ( 5 ). (frontiersin.org)

The primary defect in Dubin-Johnson syndrome is a mutation in an apical canalicular membrane protein responsible for the excretion of bilirubin and other nonbile salt organic anions. (medscape.com)

All such systems are multisubunit complexes with at least 3 dissimilar subunits embedded as a complex in the membrane (F 0 , a:b:c = 1:2:~12) and (usually) at least 5 dissimilar subunits attached to F 0 (F 1 , α:β:γ:δ:ε = 3:3:1:1:1 for F-type ATPases). (tcdb.org)

Importantly, all of these enzymes catalyzed N -glycosylation reactions in vitro with no purification or processing needed. (biorxiv.org)

Thus, glycosylation was not crucial for rhodopsin biosynthesis or trafficking. (jneurosci.org)

It has been shown in the last years that protein N-glycosylation is related to inflammatory and fibrotic processes. (amsterdamumc.org)

In this project, we will focus on N-glycosylation of AMPA-R, and we aim to elucidate their functions in synaptic transmission and plasticity. (nii.ac.jp)

H. pylori and C. jejuni are Gram-negative gastro-intestinal pathogens whose virulence is highly affected by protein glycosylation. (uwo.ca)

Interestingly, the composition of membrane-associated lipoteichoic acid was not affected. (asm.org)
Interestingly, PNGase F treatment of human brain homogenates prior to the WB, which is known to remove the N-glycosylations, unexpectedly gives rise to two dominant bands, which are now known as C-terminal (C1) and N-terminal (N1) fragments. (mdpi.com)

Dysfunction in these mechanisms, or their hijacking by viruses and toxins, is known to have serious consequences for human health, leading to congenital disorders of glycosylation, cancers, and immune dysfunction. (cornell.edu)

Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). (rndsystems.com)
No obvious hydrophobic signal sequence, membrane-spanning domains, or potential N-glycosylation sites were found in this sequence. (lu.se)

Understanding how each pathway affects virulence will reveal the best targets for the development of glycosylation inhibitors to treat these major infections. (uwo.ca)

Several mutations in the N terminus of the G-protein-coupled receptor rhodopsin disrupt NXS/T consensus sequences for N-linked glycosylation (located at N2 and N15) and cause sector retinitis pigmentosa in which the inferior retina preferentially degenerates. (jneurosci.org)

Glycosylation networks have massive potential for early detection. (vectorlabs.com)

Together, the human disease and animal models suggest that glycosylation plays a crucial role in the structure and/or function of rhodopsin. (jneurosci.org)
However, a clear role for rhodopsin glycosylation has not been established in vivo . (jneurosci.org)
The results again demonstrate that the fatty acid of Gb 3 plays a pivotal role for the overall membrane organisation. (springer.com)

The protein produced from the GALNT3 gene, called ppGalNacT3, attaches sugar molecules to fibroblast growth factor 23 in a process called glycosylation. (medlineplus.gov)

corneal calcification) or angioid streaks that occur when tiny breaks form in the layer of tissue at the back of the eye called Bruch's membrane. (medlineplus.gov)

Rather, nonglycosylated rhodopsin exhibited reduced ability to activate transducin, which was specifically associated with loss of glycosylation at N15. (jneurosci.org)

Anatomy42

Organisms11

Diseases3

Chemicals and Drugs113

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes55

Disciplines and Occupations3

Technology, Industry, Agriculture6

Information Science4

Health Care1

Proteins21

Plasma membrane7

Undergo O-linked glycosylation2

Glycoprotein2

Congenital glycosylation1

Extracellular1

Vesicles1

Lipid5

Characterization3

Biological4

Localization3

Golgi1

Inflammation2

Aberrant2

Receptors3

Antigen1

Integral1

Synthesis1

Lectin2

Retinal3

Incorporation1

Functional2

Expression3

Mucins1

Defects1

Viability1

Cell7

Intracellular1

Epitopes2

Organisms1

Apical1

Subunits1

Importantly1

Biosynthesis1

Processes1

Synaptic1

Pathogens1

Interestingly2

Immune1

Sites2

Virulence1

Preferentially1

Potential1

Role3

Gene1

Tissue1

Specifically1