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  • Antibodies
  • We compared the bactericidal activity of recombinant sets of chimeric IgG monoclonal antibodies against two important outer membrane meningococcal vaccine antigens: PorA and factor H binding protein (FHbp). (asm.org)
  • On the other hand, the IgG3 hinge-truncated antibodies IgG3m15 and IgGm17 showed higher bactericidal activity than both IgG1 and IgG3 regardless of the target antigen. (asm.org)
  • Immune protection against invasive meningococcal disease depends on recognition of bacterial surface antigens by antibodies, followed by activation of complement, leading to degradation of the bacteria by bacteriolysis, also named serum bactericidal activity (SBA). (asm.org)
  • PorA can induce bactericidal antibodies in humans and mice when they are immunized with meningococcal outer membrane vesicle (OMV) vaccines ( 4 - 8 ), and monoclonal antibodies (MAbs) against PorA can be protective in an infant rat model ( 9 ). (asm.org)
  • By using monoclonal hapten (4-hydroxy-3-nitrophenacetyl [NP/NIP])-specific antibodies of all four IgG isotypes, we have demonstrated that IgG1 and IgG3 are best in inducing complement-mediated cellular lysis and IgG1 performs better than IgG3 when the antigen concentration on the target cells is high, while IgG3 performs better than IgG1 when the antigen concentration on the target cells is low ( 20 , 21 ). (asm.org)
  • In separate studies, IgG3 antibodies also showed higher SBA than IgG1 antibodies when the target antigen was sparsely expressed (as in the case of FHbp) ( 22 ), but IgG1 antibodies were more bactericidal than IgG3 antibodies when the target antigen was highly expressed, such as for PorA ( 23 ). (asm.org)
  • For the production of antibodies against defined cell surface antigens such as these, the best approach has been described to include chemical conjugation of the antigen to a highly immunogenic carrier protein plus the use of a potent immunologic adjuvant. (aacrjournals.org)
  • For instance, an antibody deficiency can be diagnosed in the presence of low immunoglobulins, recurrent infections and failure of the development of antibodies on exposure to antigens. (wikipedia.org)
  • Check out links to articles that cite our custom service antibodies, peptides, and proteins in major peer-reviewed journals, organized by research category. (abgent.com)
  • decay-accele
  • We observed that after PMNL migration, expression of decay-accelerating factor (DAF, or CD55), the brush border-associated receptor for Afa/Dr adhesins, was strongly enhanced, increasing the adhesion of Afa/Dr DAEC bacteria. (asm.org)
  • It has already been demonstrated that Afa/Dr adhesins recognize a glycosylphosphatidylinositol (GPI)-anchored protein, complement-regulating decay-accelerating factor (DAF, also known as CD55), as a receptor ( 55 ), Interestingly, Afa/Dr DAEC strains recognize the short consensus repeat 3 (SCR3) domain of DAF, which plays a pivotal role in the regulatory function of DAF. (asm.org)
  • effector
  • Further, Double-stranded RNA-dependent protein kinase (PKR), 2', 5'-oligoadenylate synthetase (2'- 5' OAS), and adenosine deaminase acting on RNA (ADAR), known as effector proteins, complement the function of PRRs. (intechopen.com)
  • IgG1
  • IgG1 showed higher bactericidal activity than IgG3 when directed against PorA (an abundant antigen), while IgG3 was more bactericidal than IgG1 when directed against FHbp (a sparsely and variably distributed antigen). (asm.org)
  • ovarian
  • HER2 or ErbB2 is a member of the epidermal growth factor family and is overexpressed in subsets of breast, ovarian, gastric, colorectal, pancreatic and endometrial cancers. (pubmedcentralcanada.ca)
  • To characterize the safety and immunogenicity of a heptavalent antigen-keyhole limpet hemocyanin (KLH) plus QS21 vaccine construct in patients with epithelial ovarian, fallopian tube, or peritoneal cancer in second or greater complete clinical remission. (aacrjournals.org)
  • Complement-regulatory proteins in ovarian malignancies. (springer.com)
  • mitogen-activa
  • HER2 regulates signaling through several pathways (Ras/Raf/mitogen-activated protein kinase and phosphatidylinositol-3 kinase/protein kinase-B/mammalian target of rapamycin pathways) associated with cell survival and proliferation. (pubmedcentralcanada.ca)
  • outer membrane
  • Factor H binding protein (FHbp) is a lipoprotein that is sparsely distributed on the outer membrane of many meningococcal strains ( 10 - 12 ). (asm.org)
  • PorA is estimated to make up 25% of the outer membrane of meningococci, while FHbp is estimated to make up 1% ( 16 ). (asm.org)
  • After ingestion, Stx- E coli closely adheres to the epithelial cells of the gut mucosa by means of a 97-kd outer-membrane protein (intimin). (medscape.com)
  • cellular
  • Morgan, B.P., Cellular responses to the membrane attack complex. (springer.com)
  • Another two molecules of wide cellular distribution, DAF (CD55) and MCP (CD46), are expressed by lymphocytes to inactivate C3b- and C4b-containing C convertases deposited on their cell surface (reviewed in Ref. 5 ). (jimmunol.org)
  • There are two classes of viral immunoregulatory proteins: the proteins encoded by genes having sequence similarity with cellular genes and those coded by genes without any sequence similarity to cellular genes. (intechopen.com)
  • They are single-chain molecules present on host cellular membranes and belong to the complement control protein family. (medscape.com)
  • cells
  • CD46 acts as a co-factor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. (miltenyibiotec.com)
  • 5 More than 30 proteins act synergically to provide host defense against cells, microorganisms, and tissues identified as abnormal by a specific antibody. (scielo.br)
  • We show that the unfolded protein response (UPR), which is readily inducible in aggressive tumor cells, enhances melanoma or epithelial cancer cell killing upon HAdV infection. (asm.org)
  • Oncolytic viruses directly kill cancer cells and may trigger an immune response against cancer-specific or viral epitopes presented on major histocompatibility complex class I protein to immune cells. (asm.org)
  • Biologically active fragments and protein complexes derived from complement activate and damage the endothelial cells of the graft, and recruit and activate recipient blood cells, resulting in interstitial edema, hemorrhage, and thrombosis, which ultimately destroy the graft within minutes or a few hours of revascularization. (springer.com)
  • Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. (uniprot.org)
  • May act as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. (uniprot.org)
  • Moreover, T. cruzi promotes secretion of plasma membrane-derived vesicles from host cells, which prevent the activity of C3 convertase C4b2a and thereby may hinder complement. (frontiersin.org)
  • More specifically, interleukin-4 (IL-4), IL-1β, or tumor necrosis factor alpha (TNF-α) can stimulate in vitro expression of DAF in epithelial cells ( 2 , 3 , 54 , 66 , 69 ). (asm.org)
  • Down-regulation of human complement factor H sensitizes non-small cell lung cancer cells to complement attack and reduces in vivo tumor growth. (springer.com)
  • ELISA
  • ELISA: use at 0.1-1ug/ml with human CD46 on the solid phase. (qedbio.com)
  • Serologic responses by ELISA were largely IgM against each antigen with the exception of Tn-MUC1 where both IgM and IgG responses were induced. (aacrjournals.org)
  • viral
  • All these proteins are responsible for recognizing viral components and induce proinflammatory cytokine expression or interferon (IFN) response factors. (intechopen.com)
  • Molecular
  • Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
  • With state-of-the art molecular biology and protein biochemistry labs, we work with our clients to rapidly evaluate in parallel to identify the optimal expression system for candidate proteins. (abgent.com)
  • antigenic
  • These processes are associated with the production of antigenic proteins that make the virus vulnerable to immune control mechanisms 'warning' the host of the presence of an invader [ 1 ]. (intechopen.com)
  • epithelial
  • When examining the mechanism by which DAF expression was enhanced, we observed that the PMNL transepithelial migration induced epithelial synthesis of tumor necrosis factor alpha and IL-1β, which in turn promoted the upregulation of DAF. (asm.org)
  • cell
  • Cell surface complement regulatory proteins have been identified in high levels in ocular tissues, but no experimental model is available for examining their physiological roles. (readbyqxmd.com)
  • it has cofactor activity for inactivation of complement components C3b and C4b by serum factor I which protects the host cell from damage by complement. (qedbio.com)
  • The UPR-enhanced cell killing required the nuclease activity of the UPR sensor inositol-requiring enzyme 1 (IRE-1) and X box binding protein 1 (XBP-1), which alleviate ER stress. (asm.org)
  • We searched for host factors enhancing adenovirus cancer cell killing and found that the knockdown of Golgi-specific brefeldin A-resistant guanine nucleotide exchange factor 1 (GBF-1) or chemical inhibition of GBF-1 enhanced adenovirus infection by triggering the IRE-1/XBP-1 branch of the unfolded protein response (UPR). (asm.org)
  • Dalmasso, A.P., Benson, B.A., Pore size of lesions induced by complement on red cell membranes and its relation to C5b-8, C5b-9 and poly C9. (springer.com)
  • human
  • Figure B. APC conjugated Mouse anti Human CD3 ( MCA463APC ) and RPE conjugated Mouse anti Human CD46 ( MCA2113PE ). (bio-rad-antibodies.com)
  • This protein also acts as a receptor for the Edmonston strain of measles virus, human Herpes virus-6 (HHV-6), and type IV pili of pathogenic Neisseria. (qedbio.com)
  • In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. (abnova.com)
  • Gene Name
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (uniprot.org)
  • Host
  • We searched for host factors enhancing bystander effects and conducted a targeted RNA interference screen against guanine nucleotide exchange factors (GEFs) of small GTPases. (asm.org)
  • The interaction between the T. cruzi infective forms and host-related immune factors represents a key point for a better understanding of the physiopathology of CD. (frontiersin.org)
  • Each antibody is crafted with care according to rigorous protocols for immunogen design and preparation, presentation to host animal, and high-affinity purification against the antigen. (abgent.com)