Membrane bound. Medical search. Frequent questions

Protein stabilization upon ligand binding has frequently been used to identify ligands for soluble proteins. (nih.gov)
However, finding a generic method that is applicable to membrane proteins has been a challenge as the high hydrophobicity of membrane proteins and the presence of detergents essential for their solubilization interfere with fluorescence-based detections. (nih.gov)
Here the authors used MsbA (an adenosine triphosphate binding cassette transporter), CorA (a Mg(++) channel), and CpxA (a histidine kinase) as model proteins and show that DSLS is not sensitive to the presence of detergents or protein hydrophobicity and can be used to monitor thermodenaturation of membrane proteins, assess their stability, and detect ligand binding in a 384-well format. (nih.gov)
It appears that the side effects of ibuprofen overdose are related to changes in the properties of the cell membrane and, subsequently, the function of membrane-anchored target proteins. (iasp-pain.org)
Using single-molecule tracking, we show that all three Y-complex proteins exhibit three distinct mobilities, including movement through the cytosol and confined motion, predominantly at membrane-proximal sites but also within the cell center. (uni-marburg.de)
Taken together, our experiments reveal membrane-associated and membrane-independent activities of Y-complex proteins and a dynamic interplay between them with indirect membrane association of YmcA and YlbF via YaaT. (uni-marburg.de)
The biogenesis of several bacterial polytopic membrane proteins has been shown to require signal recognition particle (SRP) and protein transport machinery, and one such protein, the major light-harvesting chlorophyll- binding protein (LHCP) exhibits these requirements in chloroplasts. (soton.ac.uk)
Of the membrane proteins whose insertion into thylakoids has been analyzed, five have now been shown to insert by a SRP/Sec-independent mechanism, suggesting that this is a mainstream form of targeting pathway. (soton.ac.uk)
Kim, Soo Jung , Jansson, Stefan , Hoffman, Neil E. , Robinson, Colin and Mant, Alexandra (1999) Distinct 'assisted' and 'spontaneous' mechanisms for the insertion of polytopic chlorophyll-binding proteins into the thylakoid membrane. (soton.ac.uk)
In Escherichia coli , ribosomes must interact with translocons on the membrane for the proper integration of newly synthesized membrane proteins, cotranslationally. (rupress.org)
Recently, it has been recognized that membrane-bound ribosomes are crucial for biogenesis of integral membrane proteins in E. coli , thus renewing interest in ribosome targeting to and association with the membrane in this organism. (rupress.org)
The binding to microsomes of radiolabeled proOmpA is saturable and inhibited by the addition of unlabeled proOmpA but not by mature OmpA or other proteins. (escholarship.org)
We generated T. cruzi transfectants expressing FCaBP or Ca 2+ -binding mutant FCaBP E151Q/E188Q and recombinant wildtype and mutant proteins as well. (northwestern.edu)
We have found that the interaction between the A β proteins and the model membrane at the liquid crystal phase significantly suppresses a bending-diffusion motion with a minor effect on the LUV structure. (epj.org)
We find that binding of anionic phosphatidylglycerol (POPG) or zwitterionic phosphatidylcholine (POPC) to OmpF is sensitive to electrospray polarity while the effects of charge are less pronounced for other proteins in outer or mitochondrial membranes: the ferripyoverdine receptor (FpvA) or the voltage-dependent anion channel (VDAC). (ox.ac.uk)
Only marginal charge-induced differences were observed for inner membrane proteins: the ammonia channel (AmtB) or the mechanosensitive channel. (ox.ac.uk)
Sterol regulatory element binding proteins (SREBP) control transcription of cholesterol synthesis by regulating the transcription of 3-hydroxy-3-methyl- glutaryl coenzyme A (HMGCoA) reductase, an enzyme responsible for controllinglevels of cholesterol in the body, regulates LDL receptor which supplies cholesterol through receptor-mediated endocytosis, and also adjusts transcription of genes encoding enzymes of fatty acid synthesis and uptake. (readabstracts.com)
Recent advances that explain how cells employ an ensemble of membrane-embedded proteins to monitor sterol concentrations and adjust sterol synthesis and uptake are discussed. (readabstracts.com)
Igs, typical constitutively secreted proteins, which needed chemical cross-linking to serve as an aggregated protein control, pelleted membranes almost independently of pH. (silverchair.com)
The binding of bacteria or bacterial products to host proteins of tissue extracellular matrix may be a mechanism of tissue adherence. (nyu.edu)
The nucleus stores chromatin (DNA plus proteins) in a gel-like substance called the nucleoplasm.The nucleolus is a condensed region of chromatin where ribosome synthesis occurs.The boundary of the nucleus is called the nuclear envelope.It consists of two phospholipid bilayers: an outer membrane and an inner membrane.The nuclear membrane is continuous with the endoplasmic reticulum.Nuclear pores allow substances to enter and exit the nucleus. (coursehero.com)
2016, Thesis, Calcium-Dependent Membrane-Binding and Localization of Copine Proteins in Dictyostelium. (cmich.edu)
Platelet activation allows binding of these proteins, which bridges adjacent platelets. (medscape.com)
PUFA alter membrane physical characteristics and the activity of membrane-bound proteins. (cdc.gov)

A membrane-bound protein, is a protein that is bound (attached) to a biological membrane, may refer to: Integral membrane protein (permanently attached or built in) Peripheral membrane protein (temporarily attached) This disambiguation page lists articles associated with the title Membrane-bound protein. (wikipedia.org)
The three-dimensional structure of the membrane-bound form of the major coat protein of Pf1 bacteriophage was determined in phospholipid bilayers using orientation restraints derived from both solid-state and solution NMR experiments. (rcsb.org)
In contrast to previous structures determined solely in detergent micelles, the structure in bilayers contains information about the spatial arrangement of the protein within the membrane, and thus provides insights to the bacteriophage assembly process from membrane-inserted to bacteriophage-associated protein. (rcsb.org)
Comparisons between the membrane-bound form of the coat protein and the previously determined structural form found in filamentous bacteriophage particles demonstrate that it undergoes a significant structural rearrangement during the membrane-mediated virus assembly process. (rcsb.org)
We present a molecular dynamics simulation study on the binding mechanism of the membrane targeting domain of HIV-1 Gag protein. (elifesciences.org)
The matrix (MA) domain drives Gag onto the plasma membrane through electrostatic interactions at its highly-basic-region (HBR), located near the myristoylated (Myr) N-terminus of the protein. (elifesciences.org)
Our study suggests Myr insertion is involved in the sorting of membrane lipids around the protein binding site to prepare it for viral assembly. (elifesciences.org)
Our realistic membrane models confirm interactions with PIP 2 and PS lipids are highly favored around the HBR, and are strong enough to keep the protein bound even without Myr insertion. (elifesciences.org)
In this report we have used in vitro insertion assays to analyze four additional members of the chlorophyll-a/b-binding protein family. (soton.ac.uk)
The specific binding of [3H] ICI-198615 was dependent upon the concentration of membrane protein. (aspetjournals.org)
Here we show that in cells depleted of the SRP protein, Ffh or the translocon component SecE, the ribosomal targeting pathway is blocked downstream and unprecedented, membrane-bound FtsY-ribosomal complexes are captured. (rupress.org)
Scatchard analysis showed the Kd of proOmpA to be 7.5 X 10(-9) M. Binding is most likely protein mediated as treatment of the microsomes with the protease papain was found to be inhibitory. (escholarship.org)
These results represent the first functional characterization of secretory protein precursor binding to membranes. (escholarship.org)
Flagellar calcium-binding protein (FCaBP) is a dually acylated Ca 2+ sensor in the Trypanosoma cruzi flagellar membrane that undergoes a massive conformational change upon Ca 2+ binding. (northwestern.edu)
To our initial surprise, FCaBP E151Q/E188Q , like wildtype FCaBP, exhibited Ca 2+ -dependent flagellar membrane association, even though this protein does not bind Ca 2+ itself [16]. (northwestern.edu)
Strong binding of the LRR-containing construct was detected with the major ECM protein laminin. (ku.dk)
An amphipathic alpha-helix able to bind liposomes in vitro has been recently identified at the first 38 amino acids of the protein (amphipathic lipid-packing sensor motif), and an ARF1-binding domain (Grip-related Arf-binding domain) was found at the C-terminus. (us.es)
Lipid binding attenuates channel closure of the outer membrane protein OmpF. (ox.ac.uk)
To understand these different sensitivities, we performed an extensive bioinformatics analysis of membrane protein structures and found that OmpF, and to a lesser extent FpvA and VDAC, have atypically high local densities of basic and acidic residues in their lipid headgroup-binding regions. (ox.ac.uk)
To accomplish transcription, SREBPs should be cleaved from the membrane to enter the nucleus and this process is facilitated by mutant SREBP cleavage activation protein (SCAP). (readabstracts.com)
We investigated interactions of the plasmid-encoded outer membrane protein YadA, which confers pathogenic functions on enteropathogenic yersiniae, with fibronectin. (nyu.edu)
It was previously demonstrated that high concentrations of cholinergic agonists such as acetylcholine (ACh), carbamylcholine (CCh), suberyldicholine (SubCh) and spin-labelled acetylcholine (SL-ACh) displaced quinacrine from its high-affinity binding site located at the lipid-protein interface of the nicotinic acetylcholine receptor (AChR) (Arias, H. R. and Johnson, D. A. (1995) Biochemistry, 34, 1589-1595). (okstate.edu)
Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells. (unifi.it)

Binding of radiolabeled high affinity antagonist to leukotriene D4 receptor in guinea pig lung membranes: interconversion of agonist-receptor binding affinity states. (aspetjournals.org)
The kinetic parameters and the pharmacological specificity of a high affinity leukotriene D4 (LTD4) receptor antagonist, ICI-198615, binding to guinea pig lung membranes were characterized. (aspetjournals.org)
Several LTD4 receptor antagonists (ICI-198615, WY-48252, WY-49511, FPL-55712, and LY-171883) displaced [3H]ICI-198615 specific binding, with a rank order of potency equivalent to that described in the guinea pig tracheal smooth muscle contraction system. (aspetjournals.org)
Guanosine-5'-3-thiotriphosphate (GTP gamma S), EDTA, and Na+ shifted the LTD4 displacement curve to the right, indicating that these agents regulated the binding of LTD4 to the receptor. (aspetjournals.org)
Previous in vivo studies indicated that unlike the E. coli signal recognition particle (SRP), the SRP receptor FtsY is required for membrane targeting of ribosomes. (rupress.org)
Our results with E. coli , in conjunction with recent observations made with the yeast Saccharomyces cerevisiae , raise the possibility that the SRP receptor-mediated formation of intracellular membrane networks is governed by evolutionarily conserved principles. (rupress.org)
Secretion in yeast: preprotein binding to a membrane receptor and ATP-dependent translocation are sequential and separable events in vitro. (escholarship.org)
In addition, we show that both mPR beta and PGMRC1, two members of distinct membrane-bound progestin receptor classes, exhibit highly similar ovarian expression profiles during the reproductive cycle with maximum levels during vitellogenesis and a down-expression during late vitellogenesis. (biomedcentral.com)
Thus, in amphibians, it has been assumed for a long time, that the actinomycin D-insensitive progesterone-induced oocyte maturation [ 4 ] was mediated by a membrane steroid receptor rather than a nuclear receptor. (biomedcentral.com)
The recent cloning and sequencing of a gene coding for a membranous progesterone receptor [ 5 ] in African clawed frog ( Xenopus laevis ) brought evidence for the existence of both intracellular and membrane progestin receptors [ 6 ]. (biomedcentral.com)
Furthermore, membrane receptor capacity of the spotted seatrout ovary has been shown to be correlated with oocyte responsiveness to 17,20β,21P for maturation, assessed by the occurrence of germinal vesicles breakdown (GVBD) [ 16 ]. (biomedcentral.com)
The present results would suggest a possibility of non-receptor-mediated disorder in signaling through a modulation of a membrane dynamics induced by the association of amyloidogenic peptides on a plasma membrane. (epj.org)
A biosensor assay for studying ligand-membrane receptor interactions: Binding of antibodies and HIV-1 Env to chemokine receptors. (integralmolecular.com)
Up to 50% of these AChR antibody‒negative patients have antibodies to muscle-specific receptor tyrosine kinase (MuSK), a surface membrane enzyme that helps AChR molecules aggregate during development of the neuromuscular junction. (msdmanuals.com)
El ligando es específico para el RECEPTOR 4-1BB y puede desempeñar un papel en la indución de la proliferación de los LINFOCITOS T activados de la sangre periférica. (bvsalud.org)

In its GTP bound active form, endoplasmic reticulum membrane associated RAC1 binds to and activates KTN1 (Hotta et al. (reactome.org)
Prokaryote is the correct answer because prokaryotic cells do not have membrane-bound organelles, such as a nucleus, mitochondria, or endoplasmic reticulum. (proprofs.com)
Eukaryotes also have membrane-bound organelles, such as mitochondria and endoplasmic reticulum, which allow for more specialized functions. (proprofs.com)

Serotonin is the only putative neurotransmitter with affinity (EDso = 3 mcM) for the LSD binding site, and psychotropically active alkylindoleamines are also potent displacing agents. (erowid.org)
Two totally distinct classes of putative membrane-bound progestin receptors have been reported in vertebrates. (biomedcentral.com)
We report the discovery of a putative membrane-bound bacteriocin encoded by the Bacillus anthracis pathogenic pXO1 plasmid. (waw.pl)

However, the molecular interactions between ibuprofen and the cell membrane are not well understood. (iasp-pain.org)
This binding is facilitated by electrostatic and vdW interactions between ibuprofen and the head group of lipids. (iasp-pain.org)
Workshop on Alcohol Membrane Interactions. (ruc.dk)
These results suggest that FCaBP possesses two distinct Ca 2+ -dependent interactions - one involving a Ca 2+ -induced change in conformation and another perhaps involving binding partner association. (northwestern.edu)
In this study, we probed the interactions of four PXDN constructs with different domain compositions with components of a basement membrane extract by immunoprecipitation. (ku.dk)
Analysis of these interactions by surface plasmon resonance spectroscopy revealed similar kinetics and affinities of binding of the LRR-containing construct to human and murine laminin-111, with calculated dissociation constants of 1.0 and 1.5 μM, respectively. (ku.dk)

Like a prokaryotic cell, a eukaryotic cell has a plasma membrane, cytoplasm, and ribosomes. (coursehero.com)
They have genetic material floating freely in the cytoplasm and lack membrane-bound compartments. (proprofs.com)
Instead, it is found in the cytoplasm, where it exists as a single circular chromosome that is not enclosed by a membrane. (proprofs.com)

Eukaryotic cells are larger than prokaryotic cells and have a "true" nucleus, membrane-bound organelles, and rod-shaped chromosomes. (coursehero.com)
Eukaryotic cells have a true nucleus, which means the cell's DNA is surrounded by a membrane. (coursehero.com)
The main difference between prokaryotic and eukaryotic is that eukaryotic cells contain membrane-bound organelles, such as the nucleus, and prokaryotic cells do not. (proprofs.com)
Unlike prokaryotes, which do not have a nucleus, eukaryotic cells have a membrane-bound nucleus that contains the cell's genetic material. (proprofs.com)

We show the unique power of using small angle neutron scattering and neutron reflection as two complementary techniques to map the structural and compositional changes taken place at both lipoproteins and model membranes over time. (lu.se)

Typically, CARs consist of a target binding domain, which is an extracellular domain that is specifically expressed by CAR-T cells, a transmembrane domain, and a signalling domain, which is an intracellular domain that provides an activation signal to T cells. (nature.com)

The organization of aquaporin -4 (AQP4) into large plasma membrane assemblies provides an opportunity to image membrane -bound AQP4 antibodies (AQP4- IgG ) and evaluate changes in their spatial distribution due to alterations in AQP4 isoform expression and AQP4- IgG epitope specificity . (bvsalud.org)
Imaging secondary antibodies bound to M1-AQP4 allowed us to infer the size of individual AQP4- IgG binding events. (bvsalud.org)
A scoring algorithm was generated from these models to characterize the spatial arrangement of bound AQP4- IgG antibodies , yielding multiple epitope -specific patterns of bound antibodies on M23-AQP4 arrays. (bvsalud.org)
these antibodies bind to AChRs on the postsynaptic membrane at the neuromuscular junction and interrupt neuromuscular transmission. (msdmanuals.com)
Heterophilic antibodies, such as rheumatoid factor (RF) and human anti-mouse antibodies (HAMA), can bind to immune globulins of other species and thus interfere with the immunoassay since the tryptase immunoassay employs mouse-derived anti-tryptase. (medscape.com)

A eukaryotic cell has a true membrane-bound nucleus and has other membranous organelles that allow for compartmentalization of functions. (coursehero.com)
Because a eukaryotic cell's nucleus is surrounded by a membrane, it is often said to have a "true nucleus. (coursehero.com)
The nuclear envelope is a double-membrane structure that constitutes the outermost portion of the nucleus. (coursehero.com)
In prokaryotic cells, the DNA is "naked" and "free-floating" because these cells lack a nucleus and do not have membrane-bound organelles. (proprofs.com)

To improve antifouling and antibacterial properties of the polyimide (PI) membrane, silver particles are grafted on the membrane surface via a three-step modification route. (confex.com)
This review covers the known mechanisms of cholesterol transfer from circulating lipoprotein particles to the plasma membrane, and vice versa. (fh-ooe.at)
Wrapping anisotropic microgel particles in lipid membranes - effects of particle shape and membrane rigidity. (lu.se)

This hypothesis was confirmed by the efficient pelleting of unilamellar vesicles composed of granule membrane lipids. (silverchair.com)
Vesicles and vacuoles are membrane-bound sacs that function in storage and transport. (coursehero.com)
Other than the fact that vacuoles are somewhat larger than vesicles, there is a very subtle distinction between them: the membranes of vesicles can fuse with either the plasma membrane or other membrane systems within the cell. (coursehero.com)

Quantifying the number of charges on peptides bound to interfaces requires reliable estimates of (i) surface coverage and (ii) surface charge, both of which are notoriously difficult parameters to obtain, especially at solid/water interfaces. (insplorion.com)
Enhancement of phosphoinositide 3-kinase (PI 3-kinase) activity by membrane curvature and inositol-phospholipid-binding peptides. (tamu.edu)
Neprilysin is a circulating and membrane-bound metalloprotease that cleaves peptides. (medscape.com)

Membranes treated with sodium carbonate, pH 11, or with protease K, were still pelleted by zymogens, suggesting that the aggregated zymogens bound to membrane lipids. (silverchair.com)

We conclude that pancreatic zymogen aggregates, formed under the acidic conditions of the secretory pathway sorting compartments, have the capacity to bind firmly to membranes through their phospholipid constituents. (silverchair.com)

The accumulation of FtsY-ribosome complexes induces the formation of intracellular membranes needed for their quantitative accommodation. (rupress.org)
Recently, intracellular membrane-membrane contact sites were shown to be involved in intracellular cholesterol transfer and the plasma membrane itself has been proposed to act as a binding site for lipoprotein-mediated cargo unloading. (fh-ooe.at)

The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. (systemsbiology.net)
Here we use native mass spectrometry to explore lipid binding as a function of charge of the outer membrane porin F (OmpF). (ox.ac.uk)
Since the outer membrane is composed almost entirely of anionic lipopolysaccharide, with similar headgroup properties to POPG, such anionic lipid binding could prevent closure of OmpF channels, thereby increasing access of antibiotics that use porin-mediated pathways. (ox.ac.uk)
Both the inner and outer membranes of the nuclear envelope are phospholipid bilayers. (coursehero.com)
In addition, when platelets are activated, negatively charged phospholipids move from the inner to the outer leaflet of the membrane bilayer. (medscape.com)

The strong dependence of PI 3-kinase function on membrane curvature suggests possible localization of PI 3-kinase activity at sites where clustering of receptors, for example, may locally deform the membrane, and suggests that once PI 3-kinase is localized and activated at surface sites, the reaction may become self-accelerating. (tamu.edu)

However, the nature of the early contact of ribosomes with the membrane, and the involvement of FtsY in this interaction are unknown. (rupress.org)
We propose that in the absence of a functional SRP or translocon, ribosomes remain jammed at their primary membrane docking site, whereas FtsY-dependent ribosomal targeting to the membrane continues. (rupress.org)
Membrane-bound ribosomes in E. coli were extensively studied over 20 years ago. (rupress.org)
Mitochondria are oval-shaped, double membrane organelles that have their own ribosomes and DNA. (coursehero.com)

The ALPS motif was identified as the N-terminal binding motif and appeared concentrated in the periphery of Golgi elements and between Golgi stacks. (us.es)

In addition, they elicit rapid nongenomic responses from membrane-associated receptors. (nih.gov)
Our evidence suggests that D-[3H]LSD binds to post-synaptic serotonin receptors. (erowid.org)
Monovalent cations (Na+, Li+, and Cs+), divalent cations (Mg2+, Ca2+, and Mn2+), and guanine nucleotides did not significantly affect the specific binding of [3H]ICI-198615 to guinea pig lung LTD4 receptors. (aspetjournals.org)
In lower vertebrates, steroid-induced oocyte maturation is considered to involve membrane-bound progestin receptors. (biomedcentral.com)

While the steady-state existence in the size and shape of liquid-ordered microdomains in cell membranes, the so-called "lipid rafts", still remain the subject of debate, glycosphingolipid-cholesterol rich regions in plasma membranes have been considered to have a function as platforms for signaling and sorting. (epj.org)
Attachment of YadA-positive and YadA-negative recombinant Yersinia enterocolitica strains to cartilage-derived human cellular fibronectin and human plasma fibronectin in the solid phase revealed that YadA mediates binding of yersiniae to cellular fibronectin in a saturable, concentration- dependent manner. (nyu.edu)
By contrast, YadA failed to mediate binding to plasma fibronectin immobilized on nitrocellulose or plastic surfaces. (nyu.edu)
These observations provide evidence for the hypothesis that the binding region for YadA in cellular fibronectin is not present in plasma fibronectin. (nyu.edu)

Human soluble (S) and membrane-bound (MB) catechol O-methyltransferase (COMT, EC 2.1.1.6) enzymes have been expressed at sufficiently high levels in Escherichia coil and in baculovirus-infected insect cells to allow kinetic characterization of the enzyme forms. (lu.se)
The use of tight-binding inhibitors such as entacapone enabled the estimation of actual enzyme concentrations and, thereby, comparison of velocity parameters, substrate selectivity, and regioselectivity of the methylation of both enzyme forms. (lu.se)

Destruction of presynaptic serotonin neuronal elements by lesioning the midbrain raphe nuclei does not change the affinity or maximum number of detectable in vitro D-[3H]LSD binding sites. (erowid.org)

Coarse-grained molecular dynamics simulations, in mixed lipid bilayers, further implicate changes in charge by demonstrating preferential binding of anionic POPG over zwitterionic POPC to protonated OmpF, an effect not observed to the same extent for AmtB. (ox.ac.uk)

Concentration-dependent mechanism of the binding behavior of ibuprofen to the cell membrane: A molecular dynamic simulation study. (iasp-pain.org)
At this concentration, the physical and structural properties of the cell membrane change significantly. (iasp-pain.org)
Cholesterol is an essential component of animal cell membranes, and its concentration is tightly controlled by a feedback system that operates the transcription and posttranscriptional levels. (readabstracts.com)
To further assess the differences in agonist accessibility to the quinacrine binding site, we calculated the agonist concentration in the lipid membrane (C M ) at an external agonist concentration high enough to inhibit 50% of quinacrine binding (IC 50 ), which in turn was obtained by agonist back titration of AChR-bound quinacrine. (okstate.edu)
initial experiments established that high agonist concentrations do not affect either transmembrane proton concentration equilibria (pH) of AChR membrane suspension or AChR-bound quinacrine fluorescence spectra. (okstate.edu)

A direct correlation was observed between K p and the apparent inhibition constant (K i ) for agonists to displace AChR-bound quinacrine. (okstate.edu)

Using stimulated emission depletion nanoscopy, we imaged secondary antibody labeling of monoclonal AQP4-IgGs with differing epitope specificity bound to isolated tetramers (M1-AQP4) and large orthogonal arrays of AQP4 (M23-AQP4). (bvsalud.org)

The reaction mechanism is confirmed by Fourier Transform infra-red analysis (FTIR) and physical characterizations of the modified PI membranes. (confex.com)
It is similar to neuronal Ca 2+ sensors, like recoverin, which regulate their binding partners through a calcium acyl switch mechanism. (northwestern.edu)
By themselves, these results support the conjecture that agonist self-inhibition seems to be mediated by the quinacrine binding site via a membrane approach mechanism. (okstate.edu)

Rat membranes were pelleted by pig zymogens and vice versa. (silverchair.com)

Having complex cells in which the genetic material is organized into membrane-bound nuclei. (coursehero.com)

However, there is few evidence on the dynamics of "lipid rafts" membranes. (epj.org)

D-[3H]LSD binds saturably, reversibly, and with a high affinity (KD = 10 nM) to rat brain membranes. (erowid.org)
The regional distribution in monkey brain of D-[3H]LSD binding and high affinity [3H]serotonin uptake, a marker for pre-synaptic serotonin nerve terminal density, shows some correlation. (erowid.org)
LSD Binding corresponded to a saturable reversible high affinity site with a corresponding dissociation constant similar to that of 5-HT (KD = 2 x 10-q M) and a second, saturable and reversible site of less affinity with a corresponding ED close to 2 to 3 x 10-5 M. Various plotting systems of the binding curve indicated for the second site a positive cooperativity, the Hill coefficient being 3.7. (erowid.org)
It has been shown that melanin may bind many substances, including a variety of drugs, and has a high affinity for nicotine. (medscape.com)

Bending stiffness of biological membranes: What can be measured by neutron spin echo? (epj.org)

When pig zymogen granule membranes were mixed with the zymogens in the aggregation assay, membranes that normally floated on 1 M sucrose were observed to be pelleted by the aggregating zymogens. (silverchair.com)

The specific binding of [3H]ICI-198615 to guinea pig lung membranes was saturable and the equilibrium saturation binding was best approximated by a single-site model. (aspetjournals.org)

Orthologous to human MBTPS1 (membrane bound transcription factor peptidase, site 1). (nih.gov)

APL parameter for POPC membrane increased slightly to 0.60 and 0.63 nm in the presence of low and high concentrations of ibuprofen molecules. (iasp-pain.org)

Serotonin (5-HT) and LSD binding were studied, using purified synaptosomal membranes isolated from different regions of bovine brain by a density gradient centrifugation technique or in some experiments a lysed Pi fraction isolated either from bovine or from rat brains. (erowid.org)
Moreover, electrophysiology and mass-spectrometry-based ligand-binding experiments, at low pH, show that POPG can maintain OmpF channels in open conformations for extended time periods. (ox.ac.uk)
Further experiments might answer the question whether binding of YadA to cellular fibronectin contributes to the pathogenesis of yersiniae, both to the initial adhesion of the bacteria to the matrices of the host and to the arthritogenic potential of enteropathogenic yersiniae. (nyu.edu)
The agonist membrane partitioning experiments indicated relatively small (≤20) K p values relative to tetracaine. (okstate.edu)

Postdoc at the Dept. Biochemistry of Membranes, Utrecht University. (lu.se)

Gag clusters on membranes were identified in 10 z-stacks each containing ~10 GUVs, using an unsupervised script which calculated the average RNA fluorescence within the clusters. (elifesciences.org)
Applying fluorescence resonance energy transfer we also tested the membrane binding properties of the two lipoxygenase-isoforms, and compared their binding parameters. (univaq.it)

Esterified FA are hydrolyzed from washed RBC membranes using sequential treatment with mineral acid and base in the presence of heat. (cdc.gov)

g ) Quantitation of fluorescent RNA binding to Gag clusters. (elifesciences.org)
g ) Quantitation of fluorescent RNA binding to Gag clusters, performed as for Figure 1g but using a lower threshold of 1.25 for counting Gag clusters as positive for fluorescent RNA. (elifesciences.org)

Specific lesions of the tryptaminergic system by stereotaxic injections of 5 6-dibydroxytryptamine within raphe and anterior ventricles did not significantly modify binding of either ligand. (erowid.org)

The soluble isoform is present only in red blood cells, and the membrane-bound isoform is found in all other cell types. (medlineplus.gov)

In addition, our findings indicate that at low concentrations of ibuprofen, these molecules did not significantly alter the physical properties of the cell membrane. (iasp-pain.org)
The three-step interaction between ibuprofen and the lipid bilayer involves several events, such as the movement of ibuprofen molecules towards the central region of the lipid bilayer and the deformation and alteration of the structural and stability properties of the cell membrane. (iasp-pain.org)
Cell fractionation reveals that YaaT displays a mixture of membrane association and presence in the cytosol, while YlbF and YmcA do not show direct membrane attachment. (uni-marburg.de)
Which type of cell does not contain membrane-bound organelles? (proprofs.com)
2002). The Omega-3 Index is a measure of EPA (C20:5 n-3) and DHA (C22:6 n-3) in the blood, and expressed as a percent of 21 fatty acids, in specifically the red blood cell membranes (Harris, et al. (cdc.gov)

Molecular Membrane Biology , 12 (4), 339-347. (okstate.edu)

The antifouling effect of the silver-modified membranes is also assessed in the bovine serum albumin (BSA) aqueous solution. (confex.com)

Furthermore, the N-terminal amphipathic helix (V2-G17) tilts away from the membrane surface and becomes parallel with the transmembrane helix to form one nearly continuous long helix. (rcsb.org)
At low concentrations of ibuprofen, it can bind to the surface of the lipid bilayer. (iasp-pain.org)
Insertion events only occur with one of the membrane models, showing a combination of surface charge and internal membrane structure modulate this process. (elifesciences.org)
This negative surface provides binding sites for enzymes and cofactors of the coagulation system, resulting in the formation of a clot (secondary hemostasis). (medscape.com)

The most notable exceptions are cerebral cortical areas which are highest in D-[3H]LSD binding and only intermediate in [3H]serotonin uptake. (erowid.org)

Anatomy50

Organisms11

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Phenomena and Processes64

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