• Here we show in human cell lines that alternative 3' UTRs differentially regulate the localization of membrane proteins. (nih.gov)
  • 3' UTR-dependent protein localization has the potential to be a widespread trafficking mechanism for membrane proteins because HuR binds to thousands of mRNAs, and we show that the long 3' UTRs of CD44, ITGA1 and TNFRSF13C, which are bound by HuR, increase surface protein expression compared to their corresponding short 3' UTRs. (nih.gov)
  • The Rh blood group antigens (MIM 111700) are associated with human erythrocyte membrane proteins of approximately 30 kD, the so-called Rh30 polypeptides. (novusbio.com)
  • Heterogeneously glycosylated membrane proteins of 50 and 45 kD, the Rh50 glycoproteins, are coprecipitated with the Rh30 polypeptides on immunoprecipitation with anti-Rh-specific mono- and polyclonal antibodies. (novusbio.com)
  • Furthermore, cell-derived membrane vesicles are regarded as nano- to micrometer-sized containers comprising components such as cellular proteins, nucleic acids and lipids, for the reason that cell plasma or cytosol membranes can enclose these contents while membrane vesicles are secreted [ 3 ] . (encyclopedia.pub)
  • Additionally, BMVs consist of considerable proteins, for example, original bacterial antigens and pathogen-associated molecular pattern components [ 5 ] . (encyclopedia.pub)
  • Binding and segregation of proteins in membrane adhesion: theory, modeling, and simulations. (mpg.de)
  • Membrane-mediated cooperativity of proteins. (mpg.de)
  • CD47 is a 50 kDa membrane receptor that has extracellular N-terminal IgV domain, five transmembrane domains, and a short C-terminal intracellular tail. (wikipedia.org)
  • CD47 is a high affinity receptor for thrombospondin-1 (TSP-1), a secreted glycoprotein that plays a role in vascular development and angiogenesis, and in this later capacity the TSP1-CD47 interaction inhibits nitric oxide signaling at multiple levels in vascular cells. (wikipedia.org)
  • CD47 interacts with signal-regulatory protein alpha (SIRPα), an inhibitory transmembrane receptor present on myeloid cells. (wikipedia.org)
  • The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. (novoprolabs.com)
  • BIT, an immune antigen receptor-like molecule in the brain. (medecinesciences.org)
  • The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. (medecinesciences.org)
  • Lipopolysaccharide, in addition, induced co-clustering with Toll-like receptor 4, Fcgamma-RIIIa (CD16a) and the tetraspanin CD81 while CD47 was dissociated. (unideb.hu)
  • Here, we use a BCR light chain neoepitope defined by a characteristic point mutation (IGLV3-21R110) for selective targeting of a poor-risk subset of chronic lymphocytic leukemia (CLL) with chimeric antigen receptor (CAR) T cells. (bvsalud.org)
  • Moreover, the genetic engineering of macrophages with chimeric antigen receptor (CAR) allows them to recognize tumor antigens and perform tumor cell-specific phagocytosis. (springer.com)
  • SHPS1 Immunoglobulin-like cell surface receptor for CD47. (affbiotech.com)
  • IL-2 and IFN-γ), immune checkpoint blockade (ICB) therapy (e.g., anti-PD-1/PD-L1 antibodies), and adoptive T-cell transfer (e.g., chimeric antigen receptor (CAR) T-cell therapy) [ 3 - 5 ]. (thno.org)
  • Mechanical forces impair antigen discrimination by reducing differences in T-cell receptor/peptide-MHC off-rates. (mpg.de)
  • Membrane fluctuations and acidosis regulate cooperative binding of "marker of self" CD47 with macrophage checkpoint receptor SIRPα. (mpg.de)
  • Her study in Immunology concentrates on Complement, Inflammation, Complement receptor, Classical complement pathway and Complement membrane attack complex. (research.com)
  • CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). (wikipedia.org)
  • SIRPγ (CD172g) binds to CD47 (integrin-associated protein/IAP) that is widely expressed on most hematopoietic cells, epithelial cells, endothelial cells, fibroblasts and many tumor cell lines. (bdbiosciences.com)
  • CD47 is overexpressed on many cancer cells and binds SIRPα on immune phagocytic cells to produce a "don't eat me" signal. (pharmiweb.com)
  • A novel membrane glycoprotein, SHPS-1, that binds the SH2-domain-containing protein tyrosine phosphatase SHP-2 in response to mitogens and cell adhesion. (medecinesciences.org)
  • A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds to CD27 ANTIGEN . (nih.gov)
  • Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells. (exbio.cz)
  • A membrane-bound tumor necrosis family member found primarily on activated T-LYMPHOCYTES that binds specifically to CD30 ANTIGEN. (wakehealth.edu)
  • Treatment with a disulfide-linked antibody dimer induces apoptosis of CD47-positive primary B-CLL and leukemic cells (MOLT-4 and JOK-1). (wikipedia.org)
  • Immunotherapies with antibody-drug-conjugates (ADC) and CAR-T cells, targeted at tumor surface antigens (surfaceome), currently revolutionize clinical oncology. (lu.se)
  • The efficacy of a therapeutic antibody depends on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors.Therefore, candidates must be tested against a panel of receptors during antibody engineering. (acrobiosystems.com)
  • The majority of clinically relevant non-ABO(H) blood group antigens have been thought to drive antibody formation through T cell-dependent immune pathways. (jci.org)
  • The CD47/SIRPα interaction leads to bidirectional signaling, resulting in different cell-to-cell responses including inhibition of phagocytosis, stimulation of cell-cell fusion, and T-cell activation. (wikipedia.org)
  • By binding to SIRPα, CD47 controls hemostatic innate immune functions, such as phagocytosis and cell trafficking. (biolegend.com)
  • Membrane nanoclusters of FcγRI segregate from inhibitory SIRPα upon activation of human macrophages. (ox.ac.uk)
  • Co-ligation of SIRPα with CD47 abrogates nanocluster segregation. (ox.ac.uk)
  • SIRPγ (CD172g) may play a regulatory role in T cell-T cell interactions and may induce apoptosis through CD47. (bdbiosciences.com)
  • The long 3' UTR of CD47 enables efficient cell surface expression of CD47 protein, whereas the short 3' UTR primarily localizes CD47 protein to the endoplasmic reticulum. (nih.gov)
  • CD47 protein localization occurs post-translationally and independently of RNA localization. (nih.gov)
  • In our model of 3' UTR-dependent protein localization, the long 3' UTR of CD47 acts as a scaffold to recruit a protein complex containing the RNA-binding protein HuR (also known as ELAVL1) and SET to the site of translation. (nih.gov)
  • We also show that CD47 protein has different functions depending on whether it was generated by the short or long 3' UTR isoforms. (nih.gov)
  • CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. (wikipedia.org)
  • CD47 ( C luster of D ifferentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene . (wikidoc.org)
  • CD47, also known as Integrin-Associated Protein (IAP), is a membrane protein of about 50 kD with an IgV-like extracelluluar domain, a five membrane-spanning segment and a short terminal cytoplasmic region. (biolegend.com)
  • 50 kD plasma membrane protein of the immunoglobulin supergene family, with an extracellular IgV-like domain of 120 amino acids, a five membrane spanning segment of 152 amino acids, and a short cytoplasmic tail of 30 amino acids. (biolegend.com)
  • There are two types of phospholipid-membrane-based nanovesicles: pure lipid and/or protein vesicles and comparatively complex cell-membrane-derived vesicles (also called extracellular vesicles (EVs)) [ 1 ] . (encyclopedia.pub)
  • Natural or synthetic lipid and/or protein ingredients make nanovesicles, involving liposomes or proteoliposomes, an ideal model of the membrane system with the advantages of an easy and low-cost production [ 2 ] . (encyclopedia.pub)
  • As proof-of-concept, the established target antigen CD44 was identified as a commonly and abundantly expressed surface protein with high endocytic activity. (lu.se)
  • Importantly, we highlight the limited correlation between transcriptomics and proteomics, emphasizing the critical role of membrane protein enrichment strategies and quantitative mass spectrometry. (lu.se)
  • Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. (affbiotech.com)
  • Membrane vesicles fuse with the target cell membrane, or they bind to the receptors on the cell surface, to transfer special effects. (encyclopedia.pub)
  • In resting cells, CD14 was associated with CD55, the Fcgamma-receptors CD32 and CD64 and the pentaspan CD47. (unideb.hu)
  • Therefore, antigen-presenting cells (APCs) are able to recognize and absorb BMVs, and subsequently signaling pathways in immune cells are activated followed by specific immune responses. (encyclopedia.pub)
  • In addition, tumor-associated antigens (TAAs) produced by irradiated tumor cells can be captured by antigen-presenting cells (APCs) in the TME and presented to T cells. (nature.com)
  • Antigen-presenting cells (APCs) produce and release a broad spectrum of EVs involved in the pathogenesis of atherosclerosis. (bmrat.org)
  • They are secreted by a wide array of cells, including cardiac myocytes, mature and progenitor endothelial cells, mesenchymal stem cells, immune cells like antigen-presenting cells (APCs), and malignant cells 5 , 6 . (bmrat.org)
  • Dendritic cells (DCs) are the most important antigen-presenting cells (APCs) in the body. (biomedcentral.com)
  • DSP107 targets CD47-overexpressing tumors, simultaneously blocking macrophage inhibitory signals and delivering an immune costimulatory signal to tumor antigen-specific activated T-cells. (pharmiweb.com)
  • The most commonly used method for measuring immune cell‑mediated killing is the release assay, where effector cell‑mediated disruption of the target cell membrane results in leakage of its cytoplasmic contents into the culture medium. (technologynetworks.com)
  • If the cancer antigens are presented on licensed dendritic cells, logically, the immune system would be appropriately stimulated to attack the cancer. (shu.edu)
  • utilize multiplex fluorescent immunohistochemistry to provide a comprehensive characterization of the metastatic colorectal cancer immune microenvironment, revealing an important role for PD-L1+ antigen presenting cells in immune suppression. (jci.org)
  • Dendritic cells (DCs) are professional antigen-presenting cells that have multiple subpopulations with different phenotypes and immune functions. (biomedcentral.com)
  • For immune defense, the respiratory tract contains DCs that function as a robust antigen presentation system. (biomedcentral.com)
  • Binding of TSP-1 to CD47 influences several fundamental cellular functions including cell migration and adhesion, cell proliferation or apoptosis, and plays a role in the regulation of angiogenesis and inflammation. (wikipedia.org)
  • This facilitates interaction of SET with the newly translated cytoplasmic domains of CD47 and results in subsequent translocation of CD47 to the plasma membrane via activated RAC1 (ref. 5). (nih.gov)
  • However, these isoforms are highly conserved between mouse and man, suggesting an important role for the cytoplasmic domains in CD47 function. (wikipedia.org)
  • They are characterized by an N-terminal, extracellular IMMUNOGLOBULIN-LIKE DOMAIN and a membrane-proximal IMMUNOGLOBULIN C2-SET DOMAIN. (uchicago.edu)
  • Membrane-type 1 matrix metalloproteinase cleaves CD44 and promotes cell migration. (medecinesciences.org)
  • Types of Membrane Vesicles Acting as Tumor Vaccines" Encyclopedia , https://encyclopedia.pub/entry/36345 (accessed November 30, 2023). (encyclopedia.pub)
  • Membrane vesicles, a group of nano- or microsized vesicles, can be internalized or interact with the recipient cells, depending on their parental cells, size, structure and content. (encyclopedia.pub)
  • Academic institutions and industrial companies are putting in effort to design, modify and apply membrane vesicles as potential tumor vaccines contributing to tumor immunotherapy. (encyclopedia.pub)
  • Furthermore, bacterial membrane vesicles (BMVs), a type of cell-membrane-based EVs, which are derived from bacteria membrane architecture, own nanoscale vesicle structures containing biomembrane elements of phospholipids. (encyclopedia.pub)
  • As membrane vesicles have a general characteristic, they contain a lipid bilayer structure that can package hydrophobic and hydrophilic compounds. (encyclopedia.pub)
  • Extracellular vesicles (EVs) are a population of heterogeneous particles that originate from the endosomal system or plasma membrane. (bmrat.org)
  • Curvature-mediated assembly of Janus nanoparticles on membrane vesicles. (mpg.de)
  • CD47 is ubiquitously expressed in human cells and has been found to be overexpressed in many different tumor cells. (wikipedia.org)
  • CD47 interacts with several membrane integrins, most commonly integrin αVβ3. (wikipedia.org)
  • CD47 interacts with several membrane integrins, most commonly integrin avb3. (wikidoc.org)
  • Additionally, CD47 blocking antibodies inhibit proliferation of unstimulated astrocytoma cells but not normal astrocytes. (wikipedia.org)
  • However, apoptosis was not observed following culture with other anti-CD47 antibodies. (wikipedia.org)
  • These interactions result in CD47/integrin complexes that affect a range of cell functions including adhesion, spreading and migration. (wikipedia.org)
  • Loss of CD47 allows sustained proliferation of primary murine endothelial cells and enables these cells to spontaneously reprogram to form multipotent embryoid body-like clusters. (wikipedia.org)
  • Expression of several stem cell markers, including c-Myc, is elevated in CD47-null endothelial cells and a human T cell line lacking CD47. (wikipedia.org)
  • More recent studies suggested that DCs derived from tissues without "danger" signal stimulation should be regarded as immature DCs, based on their major role in antigen uptake and endocytosis of antigens [ 11 , 14 ]. (biomedcentral.com)
  • CD47 ligation leads to cell death in many normal and tumor cell lines via apoptosis or autophagy. (wikipedia.org)
  • The activation of CD47 induces rapid apoptosis of T cells. (wikipedia.org)
  • The apoptosis inducing function of CD47 appears to be dependent on activation of specific epitopes on the extracellular domain. (wikipedia.org)
  • Similarly, CD47 ligation rapidly induces apoptosis in B-cell chronic lymphocytic leukemia (CLL) cells. (wikipedia.org)
  • It is likely that intracellular and membrane-associated partners are crucial in determining the cellular response of CD47 signaling. (wikipedia.org)
  • CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. (novoprolabs.com)
  • Activation of CD47 with TSP-1 increases proliferation of human U-87 MG and U373 astrocytoma cells but not normal astrocytes. (wikipedia.org)
  • Though the exact mechanism is unclear, it is likely that CD47 promotes proliferation via the PI3K/Akt pathway in cancerous cells but not normal cells. (wikipedia.org)
  • Activation of CD47 with TSP-1 in wild-type cells inhibits proliferation and reduces expression of stem cell transcription factors. (wikipedia.org)
  • Role of CD47 as a marker of self on red blood cells. (medecinesciences.org)
  • CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. (exbio.cz)
  • Finally, mononuclear cells isolated from lung (L-MCs), which are used as precursors for L-DCs, expressed more antigen-presenting cell-associated markers such as MHC class II and CD172α compared to their counterparts from blood. (biomedcentral.com)
  • Proceeds will be used for advancing the Company's next generation immuno-oncology drug candidates including the clinical development of the Company's lead product, DSP107, an anti-CD47 therapy for the treatment of solid tumors through a Phase I/II study and the preclinical advancement of additional pipeline projects. (pharmiweb.com)
  • There are four alternatively spliced isoforms of CD47 that differ only in the length of their cytoplasmic tail. (wikipedia.org)
  • Due to the ubiquitous expression of CD47, signaling differs according to cell type. (wikipedia.org)
  • Vignery A. Macrophage multinucleation is accompanied by the expression of new soluble and membrane antigens in mice. (medecinesciences.org)
  • It has been reported that CD47 functions as a self marker. (biolegend.com)
  • Antigens, CD47" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
  • A ubiquitously expressed membrane glycoprotein. (rush.edu)
  • Particle-based membrane model for mesoscopic simulation of cellular dynamics. (mpg.de)
  • Membrane morphologies induced by arc-shaped scaffolds are determined by arc angle and coverage. (mpg.de)
  • Interplay of trans- and cis-interactions of glycolipids in membrane adhesion. (mpg.de)
  • Weak carbohydrate-carbohydrate interactions in membrane adhesion are fuzzy and generic. (mpg.de)