Membrane and cytoskeleton linker proteins. Medical search. Frequent questions

In 5 of 6 gene mutations that have been shown to cause EDMD, the affected protein is present in the LINC (linker of nucleoskeleton and cytoskeleton) complex. (medscape.com)
This complex includes nuclear membrane integral and associated proteins including emerin, lamin A/C, SUN1, SUN2, nesprin-1, and nesprin-2 that are proposed to form a mechanical link between the nucleoskeleton and cytoskeleton. (medscape.com)
Current models suggest that positioning of the nucleus relies on a complex called LINC (short for Linker of Nucleoskeleton and Cytoskeleton), which is made up of proteins that contain either a SUN or KASH domain. (elifesciences.org)
LINC (linkers of nucleoskeleton and cytoskeleton) complexes might be involved in this process, due to their general function in bridging the NE and thereby physically connecting the nucleus to the peripheral cytoskeleton. (uni-wuerzburg.de)
In diesem Zusammenhang könnten LINC (linkers of nucleoskeleton and cytoskeleton) Komplexe eine Rolle spielen, da ihre grundlegende Funktion darin besteht die Kernhülle zu überbrücken und somit den Kern mit dem peripheren Zytoskelett zu verbinden. (uni-wuerzburg.de)
Transmission of cytoskeletal forces to the nucleus depends highly on the LINC (linker of the nucleoskeleton and cytoskeleton) complexes, which consists of inner nuclear membrane SUN (Sad-1 and Unc) proteins that bind to outer nuclear membrane KASH (Klarsicht, ANC-1, Syne Homology) proteins. (berkeley.edu)
Using the plant Arabidopsis thaliana , also known as thale cress, as their model for non-Rabl configuration, and its mutant form with a Rabl configuration, the scientists found the protein complexes CII (condensin II) and LINC (linker of nucleoskeleton and cytoskeleton) work together to determine centromere distribution during cell division. (genengnews.com)
The linker of the nucleoskeleton and cytoskeleton (LINC) complex is formed by the conserved interactions between Sad-1 and UNC-84 (SUN) and Klarsicht, ANC-1, SYNE homology (KASH) domain proteins, providing a physical coupling between the nucleoskeleton and cytoskeleton that mediates the transfer of physical forces across the nuclear envelope. (ucdavis.edu)

LINC complexes consist of inner nuclear membrane integral SUN-domain proteins and outer nuclear membrane KASH-domain counterparts. (uni-wuerzburg.de)
Narrowing my focus on SUN proteins 1 and 2 allows me to focus on a specific component of the multifaceted LINC complex, and by studying their structures and how they interact in MD simulations, I aim to identify key differences in their roles. (berkeley.edu)
The centromere distribution for non-Rabl configuration is regulated independently by the CII- LINC complex and a nuclear lamina protein known as CRWN (crowded nuclei)," Matsunaga said. (genengnews.com)
Together with LINC, CII plays a role in scattering centromeres around the nuclear periphery during late anaphase, when the cell membrane invaginates, and telophase, when daughter cells physically separate during the terminal stages of cell division. (genengnews.com)
KASH proteins in the outer nuclear membrane comprise the cytoplasmic half of LINC complexes that connect nuclei to the cytoskeleton. (ucdavis.edu)
At the heart of this network are LINC ( li nker of n ucleoskeleton and c ytoskeleton) complexes made of SUN ( S ad1 and UN C-84) proteins at the inner nuclear membrane and KASH ( K larsicht, A NC-1, and S yne h omology) proteins in the outer nuclear membrane. (ucdavis.edu)
The LINC complex can perform distinct cellular functions by pairing various KASH domain proteins with the same SUN domain protein. (ucdavis.edu)

The first suggests that disruption of the inner nuclear membrane and the nuclear lamina causes disorganization of nuclear chromatin and gene expression, while the second proposes that the mechanical strength of the cell nucleus is disrupted when the nuclear lamina is weakened leading to structural and signaling defects in mechanically stressed tissue such as muscle and heart. (medscape.com)
The SUN proteins (SAD1, UNC-84) sit across the inner nuclear membrane and connect to structures in the nucleus, such as chromatin and the nuclear lamina, and the KASH proteins (Klarsicht, ANC-1, Syne Homology) span across the outer nuclear membrane and interact with proteins in the cytoskeleton. (elifesciences.org)

Ezrin also known as cytovillin or villin-2 is a protein that in humans is encoded by the EZR gene. (wikipedia.org)
The membrane proteins like CD44 and ICAM-2 are indirect binding partners of ezrin, while EBP50 (ERM binding protein 50) can associate with ezrin directly. (wikipedia.org)
Merlin has high homology with the ERM proteins (ezrin, radixin, and moesin) and shares an amino-terminal domain. (bmj.com)
Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. (platcovid.com)
Ezrin, a component of the undercoat of the microvilli plasma membrane. (embl.de)
Ezrin, moesin, and radixin are highly related proteins (ERM protein family), but the other proteins in which the FERM domain is found do not share any region of similarity outside of this domain. (embl.de)
Ezrin contains cytoskeleton and membrane binding domains accounting for its proposed role as a membrane-cytoskeletal linker. (google.co.in)
Ezrin has a COOH-terminal actin-binding site that is conserved in the ezrin protein family. (google.co.in)
The membrane-microfilament linker ezrin is involved in the formation of the immunological synapse and in T cell activation. (ox.ac.uk)
We report here that the membrane-microfilament linker ezrin plays an important role in these processes. (ox.ac.uk)
First, ezrin relocalizes to the contact area between T cells and stimulatory antigen-presenting cells (APCs), accumulating in F-actin-rich membrane protrusions at the periphery of the immunological synapse. (ox.ac.uk)
Second, T cell receptor (TCR)-mediated intracellular signals are sufficient to induce ezrin relocalization, indicating that this protein is an effector of TCR signaling. (ox.ac.uk)
Third, overexpression of the membrane binding domain of ezrin perturbs T cell receptor clustering in the T cell-APC contact area and inhibits the activation of nuclear factor for activated T cells (NF-AT). (ox.ac.uk)
This result suggests that phospho-ezrin is important for actin cytoskeleton regulation during erythroid cell growth and division. (biomedcentral.com)
These findings suggest that parasite proteins are able to inhibit erythroid cell growth by down-regulation of ezrin phosphorylation, leading to ineffective erythropoiesis ultimately resulting in severe malarial anaemia. (biomedcentral.com)

Moesin, which is probably involved in binding major cytoskeletal structures to the plasma membrane. (embl.de)

To date, these protein complexes are known for their essential functions in nuclear migration, anchoring and positioning of the nucleus, and even for chromosome movements and the maintenance of cell polarity and nuclear shape. (uni-wuerzburg.de)
Although a list of mitotic kinases has been implicated in NEBD, how they coordinate their activity to dissolve nuclear envelope and protein machinery such as nuclear pore complexes was unclear. (bvsalud.org)
Accession codes Atomic coordinates and structure factors for complexes of BRD4-1 with compounds 1C5 have been deposited in the Protein Data Bank (PDB) under accession codes 5F5Z, 5F60, 5F61, 5F62 and 5F63. (antiviralbiologic.com)
Such putative mechanosensors incorporate mechnosensing ion channels, cell-substrate and cell-cell junctional complexes, and cytoskeleton-associated complexes. (lckinhibitor.com)
The integrins therefore serve to hyperlink across the plasma membrane two networks: the extracellular ECM as well as the intracellular actin filamentous technique by means of multiprotein focal adhesion complexes. (lckinhibitor.com)

The N-terminal domain (also called FERM domain) binds sodium-hydrogen exchanger regulatory factor (NHERF) protein (involving long-range allostery). (wikipedia.org)
Emerin is a ubiquitous inner nuclear membraneprotein, presentin nearly all cell types, although its highest expression is in skeletal and cardiacmuscle.Emerin binds to many nuclear proteins, including several gene-regulatory proteins (eg, barrier-to-autointegration factor, germ cell-less, Btf), nesprins (proteins that act as molecular scaffolds), F-actin, and lamins. (medscape.com)
Lastly, mutations in the transmembrane protein 43 (TMEM43), also termed LUMA, which binds to emerin and SUN2, has also been reported to cause an EDMD phenotype in a few families. (medscape.com)
Binds to actin and affects the framework in the cytoskeleton. (hivinhibitor.com)
Adapter protein that binds F-actin and DNM1, and thereby plays a purpose in endocytosis. (hivinhibitor.com)
Filopodin, a slime mold protein that binds actin and which is involved in the control of cell motility and chemotaxis. (embl.de)
ERM proteins are made of three domains, the FERM domain, a central helical domain and a C-terminal tail domain, which binds F-actin. (embl.de)
For example, in Caenorhabditis elegans , SUN protein UNC-84 binds to two KASH proteins UNC-83 and ANC-1 to mediate nuclear migration and anchorage, respectively. (ucdavis.edu)
Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. (apoptosises.com)
The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. (abcam.cn)
Mab 22 binds to these linker protein (exclusion-zone filaments) and a new device for the characterisation of cytoskeleton mediated kinetoplast segregation. (isme-la2019.org)

The untethered nuclei also disrupted the network of microtubules in the cytoskeleton, and appeared much smaller and less rounded, suggesting that the cells lacked mechanical stability. (elifesciences.org)
The nucleus is translocated by actin and microtubules, yet the actual force generated by the interplay of these cytoskeletons remains elusive. (biologists.com)
The protein was part of the Y-shaped fibrous linkers between the doublet microtubules and the flagellar membrane, as indicated by statistical analysis of post-labeled sections using anti-centrin and anti-tubulin as controls. (lookformedical.com)

In wild-type worms (left), the KASH protein ANC-1 (depicted as spikes) localizes to the membrane of the nucleus and endoplasmic reticulum (ER). (elifesciences.org)
SUN- and KASH-domain proteins are directly connected to each other within the perinuclear space, and are thus capable of transferring forces across the NE. (uni-wuerzburg.de)
In addition to distinct cytoplasmic domains, the luminal KASH domain also varies among KASH domain proteins of distinct functions. (ucdavis.edu)
We show that longer KASH domains can withstand and transfer higher forces and interact with the membrane through a conserved membrane proximal EEDY domain that is unique to longer KASH domains. (ucdavis.edu)

Radixin, which is involved in the binding of the barbed end of actin filaments to the plasma membrane in the undercoat of the cell- to-cell adherens junction. (embl.de)

Cargo that can be delivered or by cytoskeleton-dependent intracellular transport mechanisms can include other proteins and complex components. (mechanobio.info)
Studies recommended that mechanosensitive channels could possibly be tethered to cytoskeletal and external anchors via intracellular and extracellular linkers. (lckinhibitor.com)
Glycans, either alone or complexed with glycan-binding proteins, can deliver intracellular signals or control extracellular processes that promote initiation, execution and resolution of cell death programs. (nature.com)

Overall, this study provides key force values and force profiles for understanding the mechanics of endocytosis and potentially other cellular membrane-remodeling processes. (biologists.com)
These effectors regulate the cytoskeleton, leading to a variety of cellular outputs including pronuclear migration, nuclear migration through constricted spaces, nuclear anchorage, centrosome attachment to nuclei, meiotic chromosome movements, and DNA damage repair. (ucdavis.edu)
Dynamic interactions between membrane and cytoskeleton components are crucial for T cell antigen recognition and subsequent cellular activation. (ox.ac.uk)
The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. (embl.de)
The cytoskeleton is a highly dynamic network of filamentous proteins that enables the active transport of cellular cargo, transduces force, and when assembled into higher-order structures, forms the basis for motile cellular structures that promote cell movement. (mechanobio.info)
All of the disease-causing ACTN4 mutations identified to date are located within the actin-binding domain of the encoded protein, increasing its binding affinity to F-actin and leading to abnormal actin rich cellular aggregates. (biomedcentral.com)
probably requires a cellular linker, for example a β-subunit. (jneurosci.org)
These proteins display interactions and are involved in the regulation of the cellular cytoskeleton. (biomedcentral.com)
It has distinct morphological features such as cellular swelling, chromatin condensation, and plasma membrane permeabilization. (bio-rad-antibodies.com)
At that time, glycobiology, which is the study of carbohydrates and their recognition by motif-specific carbohydrate-binding proteins or lectins, lagged far behind the studies that defined the structural and cellular biology of cell death. (nature.com)

ADAMTS6 Proteins Accession Important for CDC42mediated re AIM2-like receptors Proteins Species Organization with the actin cytoskeleton and for RAC1-mediated membrane ruffling. (hivinhibitor.com)
Cell membranes are highly enriched in signaling receptors, transmembrane mechanosensors, pumps and channels, and, depending on their makeup, can recruit and retain a pool of mechanosensors important in the field of mechanobiology. (mechanobio.info)
High resolution structures are available for individual receptors dimers, but less is known about receptor clusters that form in plasma membranes composed of many different RTKs with the potential to interact. (nature.com)
We find that these receptors are intermixed nonhomogenously on the plasma membrane. (nature.com)

Soak the PVDF Membrane in methanol for 1 min to activate it, and then soak the PVDF Membrane in the Transmembrane Buffer (Cat# E-BC-R333 ), the filter paper and fiber mat must be soaked in the Transmembrane Buffer for use too. (elabscience.com)
Both subunits are transmembrane proteins containing modest cytoplasmic domains, which interact with focal adhesion proteins talin, paxilin, and other folks (53, 160). (lckinhibitor.com)

We for that reason conclude that it appears probable that similarities and variations in practical classes surrounding the various biotin ligase fusion proteins can be associated to membrane proximity.PLOS 1 DOI:ten.1371/journal.pone.0117074 March 19,15 /Signaling and Trafficking Networks Surround Occludin and Claudin-Table seven. (hivinhibitor.com)
The authors began by constructing yeast strains expressing various Sla2 force sensors-Sla2 fusion proteins containing different tension sensor modules (TSMs, ref. 2) connected by mTurquoise2 and mNeonGreen fluorophores. (biologists.com)
Purified GST fusion proteins of the N and C termini of Na v 1.3 pull down contactin from lysates of transfected HEK 293 cells. (jneurosci.org)

This is certainly in contrast to the enrichment of cytoskeletal and "other" proteins enriched proximal on the Cterminus of ZO-1 [10], which is recognized to bind actin as well as other actin binding proteins [50]. (hivinhibitor.com)

Membranecytoskeletonassociated protein that promotes the assembly in the spectrin-actin network. (hivinhibitor.com)
Band 4.1, which links the spectrin-actin cytoskeleton of erythrocytes to the plasma membrane. (embl.de)

Cytoskeletal filaments provide support to the highly fluid, flexible, and extensible plasma membrane through linker proteins, 6-Carboxyfluorescein that together enable mechanical interactions with adjacent cells via cellCcell junctions or with the environment via cellCsubstrate adhesions. (pepas.org)

Experiments in C. elegans reveal new insights into how the ANC-1 protein helps to anchor the nucleus and other organelles in place. (elifesciences.org)
Here, we show that siRNA knockdown of nesprin-1, a protein present in the linker of nucleus to cytoskeleton complex, abolished the reorientation of endothelial cells in response to cyclic strain. (omeka.net)

The Nexavar TCR-CD3 complicated interacts with various other buildings at and under the plasma membrane additionally, including Compact disc4/8 co-receptor, Lck, -linked proteins of 70 kDa (ZAP-70), linker of activation of T cells (LAT), SH2 domain-containing leukocyte proteins of 76 kDa (SLP-76), phospholipase C (PLC), actin cytoskeleton, etc. (biomedigs.org)

Enriched cytoskeletal proteins tagged by biotin ligase fused to occludin and claudin-4. (hivinhibitor.com)

Ocln, Cldn4 and E-cad are all integral membrane proteins and the amino terminus of ZO-1 is positioned closer for the membrane than is definitely the carboxyl terminus. (hivinhibitor.com)
The amino-acid sequence of the FERM domain is highly conserved among ERM proteins and is responsible for membrane association by direct binding to the cytoplasmic domain or tail of integral membrane proteins. (embl.de)

This protein serves as a linker between plasma membrane and actin cytoskeleton. (wikipedia.org)
They serve as membrane-membrane, or membrane-cytoskeleton linkers, and regulate the formation of lipid domains in the plasma membrane. (rndsystems.com)
Annexins possess a series of unique 70 amino acid annexin-repeat modules that form a curved disk and allow for the creation of an intercessory complex between plasma membrane and cytofilaments. (rndsystems.com)
ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. (platcovid.com)
This is the major trafficking pathway transporting nutrients, signals, pathogens and plasma membrane components into the cell. (biologists.com)
During endocytosis, forces provided by endocytic proteins, formation of multiprotein scaffolds, protein crowding, and polymerization of the actin cytoskeleton reshape the plasma membrane into a vesicle. (biologists.com)
To transmit forces stored in the actin polymerization and crosslinking, actin filaments must be mechanically linked to the plasma membrane. (biologists.com)
These proteins bind cooperatively to the plasma membrane by N-terminal domains, and redundantly to actin filaments through C-terminal domains. (biologists.com)
FERM domains are found in a number of cytoskeletal-associated proteins that associate with various proteins at the interface between the plasma membrane and the cytoskeleton. (embl.de)
Microvillus-specific Mr 75,000 plasma membrane protein of human choriocarcinoma cells. (google.co.in)
We suppose that p210 is involved in linking basal bodies to the plasma membrane, which is an important step during ciliogenesis. (lookformedical.com)
The TWD1-ACTIN7 module controls plasma membrane presence of efflux transporters and as a consequence act7 and twd1 share developmental and physiological phenotypes indicative of defects in auxin transport. (unifr.ch)

The cytoplasmic peripheral protein encoded by this gene can be phosphorylated by protein-tyrosine kinase in microvilli and is a member of the ERM protein family. (wikipedia.org)
EDMD1 is caused by mutations in the EMD gene on the X chromosome that codes for the nuclear envelope protein emerin. (medscape.com)
They bind to structural components (emerin, nesprin), chromatin components (histone), signal transduction molecules (protein kinase C), and several gene regulatory molecules. (medscape.com)
New mutations have been found in the synaptic nuclear envelope protein 1 ( SYNE1 ) gene and in the synaptic nuclear envelope protein 2 ( SYNE2 ) gene in a few families, also termed Nesprin-1 and Nesprin-2, respectively. (medscape.com)
In 1986, the NF2 gene was mapped to chromosome 22, and in 1993, two groups independently isolated the gene and named its encoding protein merlin or schwannomin. (bmj.com)
To define genes and or proteins that contribute to the metastatic phenotype of metastasis in osteosarcoma, we have utilized a cross-species comparative approach that includes murine, canine, and human systems for gene identification and evaluation. (vin.com)
Description: Description of target: The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. (apoptosises.com)
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. (affbiotech.com)

In eukaryotic cells the genetic material is surrounded by a membrane system called the nuclear envelope (NE). (brookes.ac.uk)
As a card-carrying cell biologist, Goley was next drawn to the fledgling field of bacterial cell biology and brought her expertise in eukaryotic cytoskeletal biochemistry to studying the cytoskeleton of Caulobacter crescentus as a postdoctoral researcher with Lucy Shapiro at Stanford University. (rupress.org)
We knew, and still know, far less than for eukaryotic cytoskeletons about what these bacterial polymers really do, how their structures relate to their functions, or how they are regulated by interacting partners. (rupress.org)
Many molecular pathways in eukaryotic cells are modulated by specific signaling proteins that are controlled, by phosphorylation and dephosphorylation, through the activity of kinase and phosphatase enzymes. (biomedcentral.com)

The data presented here support a cytoplasmic integrity model where ANC-1 localizes to the ER membrane and extends into the cytoplasm to position nuclei, ER, mitochondria, and likely other organelles in place. (ucdavis.edu)

I'm using cell and molecular biology techniques, biochemistry as well as microscopy to characterise the plant SUN proteins. (brookes.ac.uk)
In my project, I use bioinformatics tools, molecular docking, and molecular dynamics (MD) simulations in collaboration with an experimental group to explore the molecular differences between these two proteins, SUN1 and SUN2. (berkeley.edu)
1. According to the molecular weight of the target protein, prepare 0% separation gel. (elabscience.com)
Add the test sample to each well, and add 5 μL of Pre-stained Protein Marker ( Cat# E-BC-R273 )to a reserved well in order to verify the target molecular weight and the extent of membrane transfer. (elabscience.com)
1. Choose the PVDF Membrane (Cat# E-BC-R266 ) with a pore size of μm according to the molecular weight of the target protein. (elabscience.com)
Molecular connections on living cells are in conjunction with various other biological processes, that are not accounted for in evaluation of purified protein (evaluate Fig. 1A and C). Unquestionably, these biological distinctions would influence the measured outcomes, but they never have been regarded sufficiently, understood, or valued. (biomedigs.org)
The just proteins that delivers data over the molecular character from the cytoskeletal component(s) in charge of positioning from the kinetoplast is normally p166, an important element of the TAC [9]. (isme-la2019.org)
Classical electroporation theory explains metastable, lipidic pores created by PEFs that enable 6-Carboxyfluorescein uncontrolled molecular and ionic transport across the cell membrane and cause a loss of cell homeostasis [27]. (pepas.org)
Our work indicates that this decisision - either to grow or to fight - on a molecular level is controlled by protein phosphorylation of ABCG36 privuded by the recepror kinase, QSK1. (unifr.ch)

Using a polyclonal antibody (anti-p210) raised against the C-terminal part of p210, it was shown that the protein was highly enriched in the basal apparatuses. (lookformedical.com)

A monoclonal antibody raised against purified flagellar basal apparatuses from the green flagellate Spermatozopsis similis reacted with a protein of 210 kDa (p210) in western blots. (lookformedical.com)

They display considerable versatility in binding modes, even between members of the same class (e.g. some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. (embl.de)

The sequence encoded a novel protein rich in alanine (25%) and proline (20%), which contained regions similar to proteins of comparable amino acid composition such as extracellular matrix components or the membrane-cytoskeletal linker synapsin. (lookformedical.com)

Regulator of protein phosphatase one (PP1), actin-binding protein. (hivinhibitor.com)
Caenorhabditis elegans protein phosphatase ptp-1. (embl.de)
for 5 min and resuspended in CelLytic M Cell Lysis Reagent (Sigma-Aldrich) containing Halt Protease Inhibitor Cocktail and Halt Phosphatase Inhibitor Cocktail (Thermo Scientific, Waltham, MA) and 5 mM EDTA at 4 C. Proteins concentrations were established with Bio-Rad Proteins Assay Reagent (Hercules, CA) and examples had been diluted with 1/3 quantity 4X SDS test buffer and warmed at 95 C for 5 min. (antiviralbiologic.com)

This model of how nuclear positioning works is primarily based on experiments in Caenorhabditis elegans worms with mutations in the genes for either the UNC-84 or ANC-1 protein ( Starr and Fridolfsson, 2010 ). (elifesciences.org)

During my PhD studies I identified two such proteins - the Sad1/Unc84 (SUN) domain proteins. (brookes.ac.uk)

The activation of these channels results in calcium (Ca2+) influx followed by membrane depolarization. (lckinhibitor.com)
Voltage-gated sodium channels mediate a rapid and transient increase in Na + permeability in response to membrane depolarization, thereby contributing to the generation and conduction of action potentials. (jneurosci.org)

This includes finding out what other proteins the SUNs bind to and what functions they have during cell division. (brookes.ac.uk)
The SUN-domain protein SUN4, which had received limited characterization prior to this work, was found to be exclusively expressed in haploid stages during germ cell development. (uni-wuerzburg.de)
Controls cell motion by regulating actin cytoskeleton homeostasis and filopodium and lamellipodium formation. (hivinhibitor.com)
Reorganization from the actin cytoskeleton and formation of cell projections through its interaction with WASL and COBL. (hivinhibitor.com)
Talin, a cytoskeletal protein concentrated in regions of cell-substratum contact and, in lymphocytes, of cell-cell contacts. (embl.de)
Once the cell has divided and entered interphase, CRWN proteins stabilize the positions of the scattered centromeres on the inner surface of the nuclear envelop, in the second step. (genengnews.com)
Membrane fusion drives pronuclear meeting in the one-cell embryo. (ucdavis.edu)
Over the T-cell membrane, the TCR-CD3 organic contains not merely the pMHC-binding stores however the Compact disc3 also, , and dimeric signaling stores. (biomedigs.org)
Cytoskeleton morphogenesis is normally central to cell routine events such as for example organelle setting, segregation, cytokinesis and mitosis [3-5]. (isme-la2019.org)
Exogenous electric fields applied as short, high-magnitude pulses cause electroporation, a phenomenon characterized by increased cell membrane permeability. (pepas.org)
In recent years there has been a growing appreciation that this cell cytoskeleton is usually involved with and affected by 6-Carboxyfluorescein PEFs [33]. (pepas.org)
Crucial cell functions such as proliferation, differentiation, signaling, migration, and cell survival would not be possible without the cell cytoskeleton [36,37]. (pepas.org)
However, our focus lies in exploring the individual roles of transport proteins driving the fascinating polar, cell-to-cell movement of the plant signaling compound, auxin. (unifr.ch)
Erin Goley investigates how the microbial cytoskeleton controls cell growth and division. (rupress.org)
As a schoolkid in North Kingstown, RI, Erin Goley's stepdad helped her build "the coolest cell in the class" with clear, self-hardening resin encapsulating various objects representing parts of the cell within a fish bowl membrane. (rupress.org)
The regulation of complex and dynamic signal transduction proteins contributes to the destination of targeting proteins and the signal transduction of cell growth, and exposure to parasites can also influence signaling pathways. (biomedcentral.com)
This role might be an adaptation specific to the unique environment at the cell cortex, or it might be fundamental, facilitating CCV formation on different membranes. (rupress.org)
Herein, we review the role of glycans and glycan-binding proteins as essential components of the cell death machinery during physiologic and pathologic settings. (nature.com)

Non-receptor tyrosine-protein kinase TYK2. (embl.de)

Specific members of the Myosin superfamily of motor proteins are known to transport cargo along actin filaments. (mechanobio.info)

When the top rated 150 enriched proteins proximal to Ocln and Cldn4 have been analyzed by division into practical classes, as previously reported for BL-ZO-1 [10] and also the E-cad-BL [11], probably the most abundant classes were these like signaling and trafficking proteins (Fig. 4). (hivinhibitor.com)
Bottom line The antigen(s) recognized by Mab 22 are cytoskeletal, insensitive to removal by high concentrations of nonionic detergent, prolong in the proximal region of basal bind and body towards the outer mitochondrial membrane. (isme-la2019.org)

Links clathrinmediated endocytosis on the actin cytoskeleton. (hivinhibitor.com)
The absence of Sla2/Hip1R-epsin linker severely impairs endocytosis. (biologists.com)
Assessing force requirements of endocytic membrane remodeling is essential for understanding endocytosis. (biologists.com)
In their work, Abella and colleagues (1) aimed to measure forces applied on the Sla2-Ent1 linker during endocytosis in yeast, using calibrated FRET-based tension sensor modules (TSMs) integrated into Sla2 protein. (biologists.com)
The similar starting point of the FRET ratio drop and its subsequent stepwise decrease observed for all 3 sensors suggests that Sla2 molecules are sequentially recruited to the growing actin cytoskeleton during the course of endocytosis. (biologists.com)
They began by following the FRET ratio during endocytic events in cells deleted for protein Rvs167 (BAR-domain protein known to bind membrane invaginations during endocytosis). (biologists.com)

Filament-forming cytoskeletal GTPase associated with actin cytoskeleton organization. (hivinhibitor.com)
Aside from binding to membranes, the activated FERM domain of ERM proteins can also bind the guanine nucleotide dissociation inhibitor of Rho GTPase (RhoDGI), which suggests that in addition to functioning as a cross-linker, ERM proteins may influence Rho signalling pathways. (embl.de)

Our special interest lies in the physical and functional interaction between the auxin exporter, ABCB1, and its regulatory interacting protein, the FKBP42, TWISTED DWARF1 (TWD1). (unifr.ch)
Phosphor-proteomics analyses uncovered S634 as a key residue of the regulatory ABCB1 linker and a very likely target of PINOID phosphorylation that determines both transporter transport activity and drug binding. (unifr.ch)
This occurs through specific modulation of regulatory proteins during the host-pathogen interaction, especially proteins with roles in pathogenesis [ 27 ]. (biomedcentral.com)

Additionally, modulation of voltage-gated ion channels and oxidization of lipids can further increase membrane permeability after PEFs [28]. (pepas.org)

Interestingly, unlike mammalian ABCB proteins - catalyzing the export of a plethora of structurally unrelated substrates, such as anti-cancer drugs - their plant orthologs were shown to own a narrow specificity towards structurally related auxinic compounds. (unifr.ch)

The role of SUN3 in the ER is reinforced by the identification of a protein interaction between SUN3 and the ER membrane-bound transcription factor maMYB. (brookes.ac.uk)

For cytoskeleton-membrane cross-linking, the dormant molecules becomes activated and the FERM domain attaches to the membrane by binding specific membrane proteins, while the last 34 residues of the tail bind actin filaments. (embl.de)

The level of protein phosphorylation at specific sites varies from less than 1% to greater than 90%, depending on conditions [ 26 ]. (biomedcentral.com)

Binding to actin filaments (via C-terminal) and to membrane proteins (via N-terminal) stabilizes the protein's conformation in its active mode. (wikipedia.org)
Adapter protein that, by means of its PDZ domain, localizes LIM-binding proteins to actin filaments. (hivinhibitor.com)

Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity. (embl.de)

This is accomplished by the force-transmitting protein linker made of endocytic adaptors Sla2-Ent1 in yeast, and Hip1R-epsin 1-3 in humans. (biologists.com)

The name annexin derives from the fact that initial studies suggested that acidic membrane phospholipids and Ca++ annexed, or recruited, special cytosolic proteins into a membrane-interacting ternary complex. (rndsystems.com)
In line with a more complex picture, multiple isoforms of the protein likely exist and non-centrosomal sites of localisation have been reported. (springer.com)
These vesicles contain the mannose 6-phosphate receptor involved in targeting proteins to the lysosome, and the actin nucleating Arp2/3 complex. (rupress.org)

The authors conclude that similar forces are applied on the Sla2 linker in WT cells and in Rvs167 mutants during initial membrane bending and early invagination, but following this, Rvs167 is crucial to facilitate productive transmission during the later invagination steps. (biologists.com)

Whatever the true mechanism, the discovery of mutations in several different nuclear membrane proteins that cause similar diseases will likely eventually lead to a better understanding of nuclear membrane physiology and the pathophysiology of diseases caused by mutations in these proteins. (medscape.com)
Deletions in the spectrin-like region of ANC-1 led to significant defects, but only recapitulated the null phenotype in combination with mutations in the trans-membrane span. (ucdavis.edu)

Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. (embl.de)

Anatomy55

Organisms10

Chemicals and Drugs105

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes54

Disciplines and Occupations2

Technology, Industry, Agriculture7

Information Science4

Health Care1

Nucleoskeleton and cytoskeleton8

LINC7

Inner nuclear membrane2

Ezrin15

Moesin1

Complexes5

Binds11

Microtubules3

KASH4

Radixin1

Intracellular3

Cellular10

Receptors4

Transmembrane2

Fusion proteins3

Actin binding1

Spectrin-actin2

Interactions1

Nucleus2

Domain-containin1

Cytoskeletal proteins1

Integral2

Plasma12

Gene8

Eukaryotic4

Nuclei1

Molecular9

Highly enriched1

Western blots1

Scaffolds1

Extracellular1

Phosphatase3

Genes1

Sad11

Depolarization2

Cell18

Kinase1

Superfamily1

Proximal2

Endocytosis6

GTPase2

Regulatory3

Permeability1

Structurally1

Interaction1

Bind actin1

Phosphorylation1

Filaments2

Ligase1

Humans1

Complex3

Mutants1

Mutations2

Amino1