• Their underlying mechanisms of action and biological activities include scavenging free radicals, modulating the nuclear factor erythroid 2-related factor 2 pathway, regulating the cell cycle, inhibiting tumor cell proliferation and migration, promoting mitochondrial pathway apoptosis, inhibiting the NF-κB and MAPK signaling pathways, regulating CD4+ T cells differentiation and associated cytokine release, inhibiting vascular smooth muscle cells, etc. (bvsalud.org)
  • Silencing of RANK and xanthoxyletin treatment declined the viability of Capan-2 pancreatic cancer cells via induction of apoptosis. (bvsalud.org)
  • Collectively, xanthoxyletin targets the RANK/RANKL signaling pathway in pancreatic cancer cells to induce cell apoptosis and may prove to be an important lead molecule. (bvsalud.org)
  • Open up in another window Shape 3 Leucine deprivation induces apoptosis of human being breast tumor BAY 293 cell(A and B) MDA-MB-231 or MCF-7 cells had been incubated in charge (+leu) or leucine-deficient (?leu) moderate for 48h, accompanied by dedication of cleaved caspases inside a and annexin-V assay in B. (C and D) Nude mice. (racetab.org)
  • In order to investigate in detail how the avian homologue ITA interfered with both TNF induced apoptosis and the NGF mediated differentiation in PC12 cells, a two hybrid screen was performed with a PC12 library using ITA as a bait. (uni-wuerzburg.de)
  • The widely studied 32D cell system was chosen to investigate the effect of NRAGE on apoptosis. (uni-wuerzburg.de)
  • Thus NRAGE was identified during this research doctorate as a novel pro-apoptotic, IAP-interacting protein, able to accelerate apoptosis in a pathway independent of Bcl-2 cell protection. (uni-wuerzburg.de)
  • It has been suggested that TRPM8 could regulate either proliferation or apoptosis mechanism in prostate cells, based on its intracellular localization [34]. (icbinhibitor.com)
  • In addition, TRPV1 has been proposed as a beneficial target for killing malignant cells, considering the fact that mitochondrial function was inhibited and apoptosis was induced in pancreatic cancer cells treated with a vanilloid analogue [8, 35]. (icbinhibitor.com)
  • We've got not too long ago reported that T-type pharmacological blockers induce apoptosis in melanoma cells, additionally to minimizing its proliferation [36]. (icbinhibitor.com)
  • discovered that inhibition of T-type channels by a selective antagonist or siRNA-mediated gene knockdown not just decreased glioma cell viability but additionally induced apoptosis. (icbinhibitor.com)
  • In most cancers, hSulf-1 is inactivated, which endows cancer cells with increasesed cell proliferation and metastatic activities, inhibition of apoptosis, and decreased sensitivity to radio- and chemotherapy. (oncotarget.com)
  • In this study, we found that hSulf-1 overexpression in melanoma cells can inhibit cell proliferation and induce cell cycle arrest and apoptosis by decreasing the protein kinase B (AKT) phosphorylation and limiting CDK4 nuclear import. (oncotarget.com)
  • The decreased expression of hSulf-1 is closely related to cancer cell proliferation, increased metastatic activity, inhibition of apoptosis, and reduced sensitivity to radio- and chemotherapy [ 3 - 5 ]. (oncotarget.com)
  • Lysosomes are an important component of the inner membrane system and participate in numerous cell biological processes, such as macromolecular degradation, antigen presentation, intracellular pathogen destruction, plasma membrane repair, exosome release, cell adhesion/migration and apoptosis. (biomedcentral.com)
  • Additionally, JIB extract induced B16/F10 cell apoptosis via the caspase cascade. (medsci.org)
  • The results demonstrated that JIB extract combined with cisplatin enhanced the inhibition of cell growth, proliferation, and survival through the obstruction of cell cycle progression and AKT/mTOR and MAPK signaling as well as the induction of cell apoptosis. (medsci.org)
  • Expression of cell cycle inhibitors, P21 and P27, and activation of apoptosis executer enzyme, caspase-3, were also induced by TCEE. (hindawi.com)
  • This TCEE also demonstrates antitumor properties such as the induction of cell cycle arrest and activation of apoptosis on human colon, lung, melanoma, osteosarcoma, and pancreatic cancer cells [ 16 - 19 ]. (hindawi.com)
  • Aloe-emodin, rhein and physcion are classified as anthraquinones, which are the main pharmacodynamic ingredients of Rheum undulatum L. . This study was undertaken to investigate whether aloe-emodin, rhein and physcion show inhibiting growth and inducing apoptosis in oral squamous cell carcinoma SCC15 cells. (biomedcentral.com)
  • We found that aloe-emodin show inhibiting growth and inducing apoptosis in oral squamous cell carcinoma SCC15 cells, we also investigated the underlying mechanisms of apoptosis induced by aloe-emodin. (biomedcentral.com)
  • Cell apoptosis was detected by flow cytometry. (biomedcentral.com)
  • Our results revealed that aloe-emodin treatment could inhibit cell viability of SCC15 cells and the potential mechanism of inhibition might be through the induction of apoptosis by regulation of the expression levels of caspase-9 and caspase-3. (biomedcentral.com)
  • Our results showed that aloe-emodin could induce SCC15 cells apoptosis, moreover, the expression levels of caspase-9 and caspase-3 increased suggesting that the potential mechanism of aloe-emodin induces apoptosis might by regulating the caspases in SCC15 cells. (biomedcentral.com)
  • The discussion covered the evaluation of cell viability, apoptosis, mitochondrial membrane potential, ATP and caspase activity and compound molecular mechanism. (acgt.co.za)
  • The expression of BCL2, an apoptosis-suppressing oncogene, is low in seborrheic keratosis in contrast to the high values in basal cell and squamous cell carcinoma. (medscape.com)
  • When Akt is inhibited, seborrheic keratosis cells quickly die through apoptosis. (medscape.com)
  • Barthomeuf demonstrated that umbelliprenin induces apoptosis in M4Beu malignant melanoma cell range (7). (colinsbraincancer.com)
  • Also the mix of IL-4 and fludarabine a nucleoside analog found in the medical administration of B-CLL causes most B-CLL cells to be less vunerable to fludarabine induced apoptosis (12). (colinsbraincancer.com)
  • Taking into consideration these characteristics today's work was made to research the induction of apoptosis by umbelliprenin in Jurkat T-CLL and Raji B-CLL cell lines. (colinsbraincancer.com)
  • Umbelliprenin induced apoptosis in Raji and Jurkat cells. (colinsbraincancer.com)
  • These results demonstrate that umbelliprenin induces apoptosis in jurkat T-CLL cells in a dose- and time-dependent manner (LC50 varies in different time incubations) (Physique 4) and that raji and jurkat cells are more susceptible to umbelliprenin induced apoptosis compared to normal PBMCs. (colinsbraincancer.com)
  • Physique 2 Annexin V-FITC/PI double staining flowcyotmetry analysis of apoptosis induction in Jurkat T-CLL cells by umbelliprenin (10 25 50 μM) after 16 and 48 h incubation. (colinsbraincancer.com)
  • Viable cells are defined as those that were … Table 1 Total apoptosis (Annexin V-FITC positive) induced by umbelliprenin in jurkat cells after 16 and 48 h incubation. (colinsbraincancer.com)
  • Error bars … drug resistance of CLL cells exposed to umbelliprenin in contrast to other drugs that induce apoptosis of CLL cells such as fludarabine. (colinsbraincancer.com)
  • Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. (lookformedical.com)
  • Sirtuins are NAD + -dependent histone deacetylases regulating important metabolic pathways in prokaryotes and eukaryotes and are involved in many biological processes such as cell survival, senescence, proliferation, apoptosis, DNA repair, cell metabolism, and caloric restriction. (biomedcentral.com)
  • There is not cured for MM. Tan II A has been reported to reduce cancer cell proliferation. (biomedcentral.com)
  • We sought to explore the possible mechanism by which Tan II A regulated cell proliferation through autophagy signaling pathway in A375 cells. (biomedcentral.com)
  • Cell proliferation was assessed by using methyl thiazol tetrazolium (MTT) assay. (biomedcentral.com)
  • We found that Tan IIA inhibited melanoma A375, MV3, and M14 cell proliferation in dose and time dependent manner. (biomedcentral.com)
  • We concluded that Tan II A reduced A375 cells proliferation by activation of autophagy production, blocked PI3K- Akt - mTOR - p70S6K1 signaling pathway, increased autophagic related gene beclin-1, LC3-II protein expressions and induced autophagocytosis. (biomedcentral.com)
  • In esophageal cancer, radiation therapy was found inducing autophagy in cancer cells, promoting cancer cell proliferation and causing treatment resistance [ 10 ]. (biomedcentral.com)
  • Other reports suggested that autophage suppressed cancer development in early stage and promoted cancer cell proliferation in later stage [ 4 ]. (biomedcentral.com)
  • Open up in another window Shape 2 Leucine deprivation inhibits breasts tumor cell proliferation(A) MDA-MB-231 or CORIN MCF-7 cells had been seeded in 12-well plates and incubated in charge (+leu) or leucine-deficient (?leu) moderate. (racetab.org)
  • Antidiabetic drug metformin has been reported to inhibit cell proliferation and metastasis in many cancers, including melanoma. (ksdb.org)
  • However, paclitaxel induced the activation of ERK/mitogen-activated protein kinase (MAPK) pathway, a cell signaling pathway implicated in cell survival and proliferation. (ksdb.org)
  • Therefore, we reasoned that combined treatment of paclitaxel with metformin could be more effective in the suppression of cell proliferation than treatment of paclitaxel alone. (ksdb.org)
  • supplied proof for T-type channel blockers as dual inhibitors of proliferation and migration of human glioblastoma cells [32]. (icbinhibitor.com)
  • It is a negative regulator of cell proliferation. (oncotarget.com)
  • In normal human tissues, hSulf-1 is stably expressed, and it plays important roles in cell proliferation and differentiation [ 1 , 2 ]. (oncotarget.com)
  • One important mechanism of carcinogenesis is uncontrolled cell proliferation caused by the excessive activation of cell proliferation signals. (oncotarget.com)
  • In addition, previous studies have shown that lysosomes may play important roles in cancer development and progression through the abovementioned biological processes and that the functional status and spatial distribution of lysosomes are closely related to cancer cell proliferation, energy metabolism, invasion and metastasis, immune escape and tumor-associated angiogenesis. (biomedcentral.com)
  • TYRO3 or AXL gene knockdown reduced cell proliferation/colony formation. (nature.com)
  • In addition, AKT/mTOR signaling and MAPK signaling were inhibited by JIB extract to suppress melanoma cell growth and proliferation. (medsci.org)
  • Functionally, ZSCAN18 overexpression inhibited the biological progression of GC cells, which was characterized by weaken proliferation, enhanced autophagy and suppressed tumor growth. (biomedcentral.com)
  • We also confirmed that ZSCAN18 high expression inhibited the proliferation of GC cells, both in vitro and in vivo. (biomedcentral.com)
  • Our further investigation revealed that ZSCAN18 could bind to tumor protein 53-induced nuclear protein 2 (TP53INP2) promoter and promote autophagy, which might be related to the inhibitory effect of ZSCAN18 on cell proliferation. (biomedcentral.com)
  • Thiazolyl blue tetrazolium bromide (MTT) test was used to detect cell proliferation. (biomedcentral.com)
  • Potential effects of forced CD20 expression on T-cell function were investigated by comparing CD20- and mock-transduced cytomegalovirus (CMV) specific T cells for cytolysis, cytokine release and proliferation. (haematologica.org)
  • Apigenin Restrains Colon Cancer Cell Proliferation via Targeted Blocking of Pyruvate Kinase M2-Dependent Glycolysis. (spandidos-publications.com)
  • Seborrheic keratoses have a variety of clinical appearances, as seen in the images below, and they develop from the proliferation of epidermal cells. (medscape.com)
  • Increased cell replication has been demonstrated in seborrheic keratoses with bromodeoxyuridine incorporation studies and immunohistochemistry for proliferation-associated antigens. (medscape.com)
  • Signal transduction plays an important role in activating cellular functions, cell differentiation , and cell proliferation . (lookformedical.com)
  • Recent studies have revealed that AXL contributes to leukaemic phenotypes through activation of oncogenic signalling pathways that lead to increased cell migration and proliferation. (cancerindex.org)
  • Although surgical resection is curative in its early stage, patients with metastatic melanoma show very poor prognosis. (ksdb.org)
  • In spite of becoming the subject of intense laboratory investigations and various clinical trials, the prognosis of metastatic melanoma is still poor. (icbinhibitor.com)
  • HCM-BROD-0335-C43 ( ATCC PDM-293 ) was isolated from metastatic melanoma of skin tissue. (atcc.org)
  • In mammals, PTCH is an important inhibitor in the so-called hedgehog (HH) signaling pathway, whose downstream proteins can lead to cell growth. (medscape.com)
  • Nonetheless, the anticancer effects of xanthoxyletin against human pancreatic cancer cells have not been evaluated. (bvsalud.org)
  • Against this backdrop, the present study was designed to evaluate the anticancer effects of xanthoxyletin in human pancreatic cancer cells and to decipher the underlying molecular mechanisms. (bvsalud.org)
  • The administration of pancreatic cancer cells with xanthoxyletin diminished the viability of Capan-2 cells in a concentration-dependent manner and led to a significant decline in RANK, RANKL, and OPG expression. (bvsalud.org)
  • However, pancreatic cancer cells overexpressing RANK could rescue the growth inhibitory effects. (bvsalud.org)
  • Here, we tested whether ALKBH6 plays a significant role in preventing alkylating DNA damage and decreasing genomic instability in pancreatic cancer cells. (frontiersin.org)
  • In particular, the loss of ALKBH6 in human pancreatic cancer cells increases alkylating agent-induced DNA damage and significantly decreases cell survival. (frontiersin.org)
  • Overall, our data suggest that ALKBH6 is required to maintain the integrity of the genome and promote cell survival of pancreatic cancer cells. (frontiersin.org)
  • The extremely low 5-year overall survival of patients is, in part, due to pancreatic cancer cells having several mechanisms of resistance to different chemotherapeutic treatments, one of which is their capacity to efficiently repair alkylating agent-induced DNA damage. (frontiersin.org)
  • Cytotoxic effect of Iso-mukaadial acetate on colon and pancreatic cancer cells. (acgt.co.za)
  • Hypoxia is considered a critical contributor to renal cell carcinoma progression, including invasion and metastasis. (biomedcentral.com)
  • The purpose of this study was to investigate the role and mechanism of hypoxia-induced renal cell carcinoma and provide evidence-based medical proof for improvements to postoperative nursing of renal cell carcinoma patients. (biomedcentral.com)
  • A total of 64 patients with renal cell carcinoma were divided into the observation group (nursing based on oxygen administration) and the control group (conventional nursing). (biomedcentral.com)
  • The human renal cell carcinoma cell line A498 under hypoxia/normoxia was used as an experimental model in vitro and the biological characteristics and mitochondrial function of the cells were assessed. (biomedcentral.com)
  • Nursing based on oxygen administration decreased the value of renal function indexes, serum inflammatory factors, and tumor markers in renal cell carcinoma patients. (biomedcentral.com)
  • These data reveal that nursing based on oxygen administration can improve the clinical efficacy of renal cell carcinoma therapies, being safe and effective. (biomedcentral.com)
  • The results elucidate a mechanism wherein the solute carrier family 14 member 1 gene participates in the occurrence and development of hypoxia-induced renal cell carcinoma in a mitochondria-dependent manner. (biomedcentral.com)
  • Renal cell carcinoma (RCC) originates from either the cancerous renal cortex or renal tubular epithelial cells, and is the second most lethal urological malignancy, accounting for 90-95% of kidney neoplasms. (biomedcentral.com)
  • PD-L1 are expressed on the cell surface of numerous cancers, including bladder, melanoma, renal cell carcinoma, lung cancer and ovarian cancer characterizing an important step toward cancer progression (Wang et al. (biomedcentral.com)
  • Clear cell renal cell carcinoma (ccRCC) is the seventh most frequently diagnosed tumor in adults in Europe and represents approximately 2.5% of cancer deaths. (cancerindex.org)
  • The molecular biology underlying renal cell carcinoma (RCC) development and progression has been a key milestone in the management of this type of tumor. (cancerindex.org)
  • We tested the effects of Tan II A on melanoma A375, MV3, M14, and other human cell lines including Hacat and HUVEC cells in cell culture model. (biomedcentral.com)
  • Cell migration ability melanoma A375 was monitored by using cell scratch assay. (biomedcentral.com)
  • Transwell chamber experimental was performed to assess the effect of Tan II A on A375 melanoma cell invasion ability. (biomedcentral.com)
  • The effects of Tan II A on tumor progression was also examined in melanoma A375 induced tumor in mouse model. (biomedcentral.com)
  • Tan II A reduced CXCL12-induced A375 cell invasive ability and migration in a dose dependent manner. (biomedcentral.com)
  • Tan IIA promoted autophagic body production and increased autophagy-associated protein beclin-1 and LC3-II expression in A375 cells. (biomedcentral.com)
  • We also confirmed that Tan II A reduced melanoma A375 induced tumor volume and weight in mouse model. (biomedcentral.com)
  • Tan II A inhibited melanoma A375 induced tumor development in mouse model. (biomedcentral.com)
  • Four varieties of tumor cells were examined: malignant melanoma A375, lung caner A549, ovarian carcinoma breast and A2780 cancer MCF-7 and MDA-MB-231 cells. (racetab.org)
  • Besides, 12% of patients have second- or third-degree skin reactions that are sensitive to light, and about 18% of patients develop cutaneous squamous-cell carcinoma and keratoacanthoma or occurred at the same time [ 6 ]. (medsci.org)
  • Therefore, in this study, we examined the effect of aloe-emodin, rhein and physcion on the growth of human oral squamous cell carcinoma cell line SCC15. (biomedcentral.com)
  • SUMMARY BLURB Essential oil derived from the camphor tree acts by stimulating immune cell-dependent regression of skin tumors in a mouse model of cutaneous squamous cell carcinoma. (biorxiv.org)
  • Surgery to remove the tumor and chemotherapy are the routine treatments for early-stage melanoma. (biomedcentral.com)
  • Several alkylating agents are commonly used in chemotherapy but may induce DNA damage to cancer cells as well as normal cells. (frontiersin.org)
  • The current treatments for melanoma are surgery, which is performed in the early stages of the disease, and neoadjuvant treatments for advanced patients, including chemotherapy, radiotherapy, targeted therapy, immunotherapy and combination therapy [ 2 , 3 ]. (medsci.org)
  • The aims of this study are to clarify the antitumor effects of TCEE on human hepatocellular carcinoma cells and also evaluate the combination drug effects with conventional chemotherapy agents, cisplatin and doxorubicin. (hindawi.com)
  • In contrast, the antitumor effects and related biological mechanism of TCEE as well as the combination drug effects with conventional chemotherapy agents remain unclear particularly in human hepatocellular carcinoma cells. (hindawi.com)
  • Furthermore, the combined drug effects of TCEE with conventional chemotherapy agents, cisplatin and doxorubicin, were also analyzed to clarify whether TCEE enhances or antagonizes the cytotoxicity of the selected chemotherapy agents in hepatocellular carcinoma cells. (hindawi.com)
  • Unfortunately, the traditional treatment methods of chemotherapy and radiotherapy have proven to be ineffective and lead to multidrug resistance in target cells. (biomedcentral.com)
  • Besides surgery, radiotherapy and chemotherapy, immune activation by direct application of cytokines, antibodies or adoptive cell therapy are promising approaches. (frontiersin.org)
  • Recent developments in cancer biology have identified the existence of a sub-poplulation of cells - cancer stem cells (CSC) that are resistant to most traditional therapies (e.g. chemotherapy and radiotherapy) and have the ability to repair their damaged DNA. (docksci.com)
  • Aloe-emodin treatment resulted in a time- and dose-dependent decrease in cell viability and increased the apoptotic cell ratio. (biomedcentral.com)
  • hSulf-1 reduces the sulfation of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix and inhibits binding between various cell growth factors and their receptors. (oncotarget.com)
  • The purpose of BAY 293 our current research would be to investigate the result of leucine deprivation on breast tumor cells and also to elucidate the root mechanisms. (racetab.org)
  • however, mechanisms through which these bioactive compounds act on epithelial cells have not been identified. (biorxiv.org)
  • Our results suggest that one of the mechanisms related to the success of BCG in reducing tumor recurrence in NMIBC may be related to the negative control of PD1 and PD-L1 in tumor cells. (biomedcentral.com)
  • Conversely, malignant cells evolve mechanisms that allow them to evade immune recognition. (biomedcentral.com)
  • They have complementary mechanisms to hinder the carcinogenic process by searching free radicals, inhibiting survival and multiplication of malignant cells, as well as reducing invasiveness and angiogenesis of tumors. (researchsquare.com)
  • One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). (lookformedical.com)
  • Moreover, treatment with TCEE is found to enhance the cytotoxic effects of amphotericin B in human colon cancer cell both in vitro and in vivo [ 17 ]. (hindawi.com)
  • In vivo , CWO induced transcriptional changes in immune-related genes, resulting in cytotoxic T cell-dependent tumor regression. (biorxiv.org)
  • In the last years, adoptive transfer of natural killer (NK) cells came into the focus of translational medicine, because of their high cytotoxic potential against transformed malignant cells. (frontiersin.org)
  • Hence, we established a two-phase expansion protocol with IL-15 to induce an early NK cell expansion, followed by short exposure to IL-21 that boosted the cytotoxic activity of NK cells against RMS cells. (frontiersin.org)
  • Th1 cytokines (IL-2, tumor necrosis factor, IL-12, and IFN-γ) and Th2 cytokines (IL-4, IL-5, IL-6, and IL-10) along with IL-8 and IL-17 induce antitumor activity mediated by cytotoxic T lymphocytes, natural killer cells (NK), neutrophils, and macrophages. (biomedcentral.com)
  • These therapeutic methods, including but not limited to oncolytic virus therapies, T-cell therapies and cancer vaccines, are based on the body's ability to recognize mutated antigen peptides presented on the cell surface by MCH-receptors (also known as HLA-receptors in humans) and the disposal of the malignant cells by cytotoxic T-cells. (helsinki.fi)
  • We further confirmed that hSulf-1 overexpression can inhibit AKT phosphorylation and CDK4 nuclear localization and retard the growth of melanoma xenograft tumors in nude mice. (oncotarget.com)
  • Dendritic cells (DCs) are a heterogeneous population of immune cells that influence a wide variety of immune responses, including immunity to infectious diseases and malignant tumors, and in the generation of tolerance. (pitt.edu)
  • Daily topical treatment with CWO induced dramatic regression of pre-malignant skin tumors and a two-fold reduction in cutaneous squamous cell carcinomas. (biorxiv.org)
  • Here, we investigated the combinatory effect of paclitaxel and metformin on the cell survival in SK-MEL-28 melanoma cell line. (ksdb.org)
  • Our study shows that the combination of paclitaxel and metformin has synergistic effect on cell survival and suppresses the expression of proteins involved in cancer metastasis. (ksdb.org)
  • VGCCs also play a relevant role within the survival of cancer cells. (icbinhibitor.com)
  • The survival rate of melanoma patients with increasing stage ranges from 85% to 25% due to the high incidence of metastasis [ 1 ]. (medsci.org)
  • In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
  • Sensitization of cancer cells to conventional drugs using multi-target agents that block survival and oncogenic pathways, alone or in combination, is an emerging strategy to overcome drug resistance. (researchsquare.com)
  • The activation of Akt kinase enhances the survival of cells by blocking the p53 pathway and the FOXO-mediated proapoptotic cascade. (medscape.com)
  • Cell survival. (lookformedical.com)
  • It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION . (lookformedical.com)
  • Oral and pharyngeal cancer is the most common malignant human cancers. (biomedcentral.com)
  • Finally, cell fate is also dependent on Ca2+ 587850-67-7 Epigenetics influx and its molecular machinery. (icbinhibitor.com)
  • Overall, hSulf-1 function in melanoma cells provides an ideal molecular treatment target. (oncotarget.com)
  • JIB extract induced cell cycle arrest at the G 0 /G 1 phase and decreased cyclin and cdk protein expressions. (medsci.org)
  • In the present study, the TCEE treatment induced cell cycle arrest and suppressed cell growth on both Hep3B and HepJ5 cells. (hindawi.com)
  • The complete cDNA for this gene was isolated, the analysis of which revealed a high homology with a large family of tumour antigens known as MAGE (melanoma associated antigens). (uni-wuerzburg.de)
  • MAGE proteins are usually restricted in their expression to malignant or tumour cells, however NRAGE was also expressed in terminally differentiated adult tissue. (uni-wuerzburg.de)
  • A novel human anti-AXL monoclonal antibody attenuates tumour cell migration. (cancerindex.org)
  • AXL significantly promotes tumour cell migration, invasion and metastasis, as well as angiogenesis. (cancerindex.org)
  • The biological function of ZSCAN18 in GC cells was verified by in vitro and in vivo experiments. (biomedcentral.com)
  • Furthermore, the knockdown of TP53INP2 alleviated the inhibiting effects of ZSCAN18 in GC cells by in vitro and in vivo experiments. (biomedcentral.com)
  • These phytochemicals have been tried against cancer viability at both in vitro and in vivo levels. (researchsquare.com)
  • FGFR3 belongs to a class of transmembrane tyrosine kinase receptors involved in signal transduction to regulate cell growth, differentiation, and migration, as well as wound healing and angiogenesis. (medscape.com)
  • Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. (lookformedical.com)
  • Still another object of the present invention is to provide a nucleic acid molecule encoding the antibody or antigen-binding fragment thereof, a recombinant expression vector containing the nucleic acid molecule, and a cell transformed with the recombinant expression vector. (justia.com)
  • Chondroitin sulfate proteoglycan 4, also known as melanoma-associated chondroitin sulfate proteoglycan (MCSP) or neuron-glial antigen 2 (NG2), is a chondroitin sulfate proteoglycan that in humans is encoded by the CSPG4 gene. (wikipedia.org)
  • This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. (cancerindex.org)
  • The use of CD20 as suicide gene was investigated in CMV specific T cells and in T cells genetically modified with an antigen specific T-cell receptor. (haematologica.org)
  • T-cell receptor (TCR) gene transfer is an attractive strategy for rapid in vitro generation of high numbers of antigen specific T cells. (haematologica.org)
  • Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
  • Dendritic cells (DCs) are specialized antigen-presenting cells that have a notable role in the initiation and regulation of innate and adaptive immune responses. (mdpi.com)
  • Urothelial and bladder tumor cells infected by BCG increases the expression of antigen-presenting molecules, inducing immune response via cytokine release. (biomedcentral.com)
  • Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)
  • For AgL20, AuL20, AgM1 and AuM1, the Half-Maximal Inhibitory Concentration (IC50) values were measured, and all complexes seemed to reduce cell viability more effectively than Cisplatin, selected as control. (unibas.it)
  • According to the JIB extract's anti-melanoma capacity, to assess the synergistic effects of cisplatin and JIB extract. (medsci.org)
  • Collectively, our results indicate that JIB extract showed anti-tumor effects and synergized with cisplatin against B16/F10 cells, indicating the possibility of JIB extract to be developed as adjuvant therapy for melanoma. (medsci.org)
  • They possess proven that M4Beu cells aren't only more vunerable to umbelliprenin weighed against cisplatin but will also be markedly more delicate to the coumarin than diploid fibroblasts (7). (colinsbraincancer.com)
  • Malignant melanoma (MM) is one of the high degree of malignancy and early prone to blood and lymph node metastasis. (biomedcentral.com)
  • Mr Brenden Moyo from the University of Johannesburg presented research findings on the potential impact of medicinal plant extracts in treatment of human malignant melanoma and cervical cancer. (acgt.co.za)
  • Autoimmune side effects, however, remain a risk, emphasizing the relevance of a suicide mechanism allowing in vivo elimination of infused T cells. (haematologica.org)
  • Adverse side effects, however, may occur and lead to severe autoimmunity or graft-versus-host disease (GvHD), emphasizing the relevance of a suicide mechanism that allows efficient in vivo elimination of infused T cells. (haematologica.org)
  • Transfer of HSV-tk to DLI preserves the beneficial anti-tumor effect and allows in vivo elimination of donor T cells if severe GvHD occurs. (haematologica.org)
  • Therefore, ex vivo NK cell expansion protocols are currently being developed. (frontiersin.org)
  • In a proof of concept in vivo study, we also observed a therapeutic effect of adoptively transferred IL-15 expanded and IL-21 boosted NK cells in combination with image guided high precision radiation therapy using a luciferase-transduced RMS xenograft model. (frontiersin.org)
  • In summary, this two-phased feeder cell-free ex vivo culturing protocol combined efficient expansion and high cytolytic functionality of NK cells for treatment of radiation-resistant RMS. (frontiersin.org)
  • In this context, cytokines have been shown to activate NK cells potently during ex vivo expansion ( 9 - 12 ). (frontiersin.org)
  • Then the in vivo sensitivity of these transfected C6-TK and C6-sr39TK cells to GCV was determined by 3-(4,5)-dimethylthiahiazo-(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) colorimetric assay and Hoechst-propidium iodide (PI) staining. (hindawi.com)
  • It has been shown to be expressed in the setting of inflammation and/or on tumor cells of distinct origin (Seliger, 2019 ). (biomedcentral.com)
  • Overexpression of the solute carrier family 14 member 1 gene could abolish hypoxia-induced invasion, reduce the migration of A498 cells, inhibit the hypoxia-induced release of inflammatory cytokines, and arrest the cell cycle at the G1/S checkpoint. (biomedcentral.com)
  • The expression of HIF-1 downstream genes, cancer colony formation, and Matrigel invasion of glioblastoma cells were stimulated by conditioned medium from astrocytes pre-exposed to hypoxia. (cancerindex.org)
  • Title: Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles Abstract: Cancer multidrug resistance (MDR) is one of the main challenges for cancer treatment efficacy. (up.pt)
  • It finds that human osteosarcoma and human lung carcinoma metastasized to bone marrow cell viability decreased to 0% when treated with cNPs. (emerginginvestigators.org)
  • We examined TLR3- and TLR7-expressing pancreatic adenocarcinomas, squamous cell carcinomas of head and neck and lung carcinomas. (aacrjournals.org)
  • But when the experiment was repeated with cancerous cells only a tiny percentage were left alive. (thenakedscientists.com)
  • Die Untersuchung der isolierte Gesamt-cDNA ergab eine hohe Homologie mit einer Familie von Tumor-Antigenen namens MAGE (melanoma associated antigens). (uni-wuerzburg.de)
  • Cell-associated antigens are of special interest because they play a role in both the induction of immunity and tolerance. (pitt.edu)
  • 1 , 2 The anti-tumor effect of DLI is mediated by donor T cells recognizing allo-antigens on the malignant cells of the recipient. (haematologica.org)
  • Donor T cells may also induce GvHD due to recognition of allo-antigens on non-malignant tissues of the patient. (haematologica.org)
  • 4 In addition, TCRs for minor histocompatibility antigens, including HA-1 and HA-2, have been transferred to donor-derived virus-specific T cells to treat patients with hematologic malignancies after allogeneic stem cell transplantation. (haematologica.org)
  • They can be activated rapidly via germ-line encoded receptors that recognize the presence of stress ligands or absence of self-antigens on target cells ( 1 - 5 ). (frontiersin.org)
  • This master thesis is a part of a project aiming to set up immunoaffinity-purification/MS based method in order to analyse the ligandome and determine T-cell recognized cancer associated antigens from tumor cells. (helsinki.fi)
  • Specialists apply the term angiosarcoma to a wide range of malignant endothelial vascular neoplasms that affect a variety of sites. (medscape.com)
  • PD-L1 is a ligand of the co-inhibitory receptor PD1 that has been shown to be expressed by tumor cells of distinct origin related to unfavorable prognosis. (biomedcentral.com)
  • Hereon, this evaluation will discuss the current understanding concerning the role of unique Ca2+ channels expressed in the plasma membrane of melanoma cells, at the same time because the Ca2+ signaling pathways involved in the course of tumorigenesis and tumor progression.2. (icbinhibitor.com)
  • 5) Lysosomes can also fuse with the plasma membrane to mediate membrane repair or discharge contents outside the cell, such as cathepsins or immune factors. (biomedcentral.com)
  • To quantitatively and qualitatively characterize uptake, we created a biologically relevant system using the Epstein Barr virus latent membrane protein 2 and the melanoma protein gp100, each fused to the enhanced green fluorescent protein (EGFP) and expressed at the outer plasma membrane of a tumor cell line, along with a cell line expressing EGFP in the cytoplasm. (pitt.edu)
  • The aims of this preclinical study are to evaluate the capability of TCEE to suppress human hepatocellular carcinoma cells and clarify the related antitumor effects. (hindawi.com)
  • CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. (wikipedia.org)
  • The researchers also demonstrate that the induced pluripotent stem cells (iPSCs)-derived OPCs from CSPG4A131T mutation carriers exhibited abnormal post-translational processing, subcellular localization of the mutant NG2 protein, aberrant cellular morphology, and a decreased cell viability and myelination potential. (wikipedia.org)
  • Differentiating between low-grade lymphoma and reactive lymphocytes is often difficult by morphology alone as reactive lymphoid cells may acquire activation morphology from being exposed to different cytokines within the body fluid. (cytojournal.com)
  • During tumor development, stromal cells are co-opted to the tumor milieu and provide favorable conditions for the tumor. (cancerindex.org)
  • When the cell's DNA and protein damaged, the cells maintained its cellular homeostasis through autophagy. (biomedcentral.com)
  • We hypothesized that the DCs ability to capture, internalize, and process integral membrane proteins would vary based on the target cell's viability and that the DCs ability to capture cell-associated protein would vary based on the protein's intracellular localization. (pitt.edu)
  • Peripheral blood mononuclear cells (PBMC) were collected from healthy donors after approval by the LUMC institutional review board and informed consent according to the Declaration of Helsinki. (haematologica.org)
  • We analyzed the expression of PD1 and PD-L1 using qRT-PCR in RT4 bladder cancer (BCa) epithelial cells co-cultivated with peripheral blood mononuclear cells (PBMC) after treatment with BCG. (biomedcentral.com)
  • Alpha-pinene showed anti-inflammatory properties in human peripheral blood mononuclear cells and mouse macrophages through inhibition of tumor necrosis factor-α, interleukin-1β, nitric oxide, and mitogen activated protein kinases. (richardcyoung.com)
  • Calebin-A induced death of malignant peripheral nerve sheath tumor cells by activation of histone acetyltransferase. (cancerindex.org)
  • This channel is strongly expressed in poorly metastatic B16 cellsBioMed Investigation InternationalCa2+ Ca2+ORAITRPMSTIM1 T-type ERSOCECa2+Ca2+ TRPM8/7/2 SurvivalAutophagyProliferationMigration, invasion, and metastasisApoptosisFigure 1: Ca2+ -influx pathways and their physiological functions in melanoma cells. (icbinhibitor.com)
  • Hypoxia was found to induce A498 cell invasion, migration, and the release of inflammatory cytokines, while repressing human solute carrier family 14 member 1 gene expression. (biomedcentral.com)
  • Outcomes Leucine deprivation decreases viability of tumor cells We 1st determined the result of leucine deprivation on viability of tumor cells. (racetab.org)
  • The TAM receptor and ligand expression was evaluated in LMS cell lines and 358 sarcoma samples by either gene expression or immunohistochemistry. (nature.com)
  • One recent study 29 confirmed that frankincense essential oil is bioactive and affects human gene expression, upregulating at least 42 and downregulating 41 genes, stating that "Many of these signaling pathways are closely related to the biological processes of inflammation, immune response, and tissue remodeling in human cells. (richardcyoung.com)
  • The signature of our top screening hits is significantly correlated with gene expression data from clinical melanoma samples. (cdc.gov)
  • Two non-commercial metallic Au-based complexes were tested against one of the most aggressive malignant melanomas of the skin (MeWo cells), through cell viability and time-lapse live-cell imaging system assays. (unisa.it)
  • Immunoblotting, reporter assays, quantitative RT-PCR, and chromatin immunoprecipitation were performed to evaluate HIF-1 signaling in glioblastoma cells. (cancerindex.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • In most cancer cell lines and tumor tissues, hSulf-1 expression levels are markedly decreased. (oncotarget.com)
  • This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. (lookformedical.com)
  • Laser scanning confocal microscopy is a significant advance in the field of optical microscopy, primarily because it permits sample visualization deeper within living and fixed cells, tissues and other samples. (sheffield.ac.uk)
  • T-cell clones were stimulated every 10-14 days with irradiated allogeneic PBMC and phyto-hemagglutinin in IMDM with 5% human serum (HS), 5% FBS and 100 IU/mL IL-2 as previously described. (haematologica.org)
  • Numbers in each quadrant indicate the percentage of cells labeled with annexin … Tyrphostin AG 879 Figure 3 Relative percent viability of cells (Jurkat Raji PBMC) in the presence of umbelliprenin after 48 h incubation. (colinsbraincancer.com)
  • Preliminary MTS assay is used to determine linear range and optimal time frame for the PBMC/cancer cell co-culture killing assay. (helsinki.fi)
  • Moreover, it has been shown thatBioMed Investigation International T-type calcium channels regulate cell motility and migration in fibrosarcoma cells [31]. (icbinhibitor.com)
  • Calcium Channels in MelanomaCutaneous melanoma is often a malignant skin cancer that arises from transformed melanocytes de novo or from dysplastic, congenital, or widespread nevi [50]. (icbinhibitor.com)
  • These findings suggest that the combination of paclitaxel and metformin can be a possible therapeutic option for treatment of melanoma. (ksdb.org)
  • CD20-transduced T cells with and without co-transferred T-cell receptor were efficiently eliminated by complement dependent cytotoxicity induced by therapeutic anti-CD20 antibody rituximab. (haematologica.org)
  • New therapeutic concepts in anti-tumor therapy aim to modulate the patient's immune system to increase its aggressiveness or targeted effects toward tumor cells. (frontiersin.org)
  • One of the possible reasons for the failure of the present therapeutic regimens is the existence of a relatively rare cell population known as cancer stem cells (CSCs) that are resistant to or not targeted by present therapeutic modalities. (docksci.com)
  • Therefore, In order to reduce the GCV dose required, we aim to compare the therapeutic efficacy of HSV1-sr39TK, an HSV1-TK mutant with increased GCV prodrug catalytic activity, with wildtype TK in C6 glioma cells. (hindawi.com)
  • Most importantly, the knockdown of TP53INP2 alleviated the inhibiting effects of ZSCAN18 overexpression in GC cells. (biomedcentral.com)
  • There was a significantly reduction in PD1 and PD-L1 expression by BCa epithelial cells after BCG treatment. (biomedcentral.com)
  • Selectivity was further checked on a non-cancerous cell line, a spontaneously transformed aneuploid immortal keratinocyte from adult human skin (HaCaT). (unibas.it)
  • In such a case, AuM1 and PheAg complexes resulted as the most selective allowing HaCaT viability at 70 and 40%, respectively, after 48 h of treatment at 5 & mu;M. The same complexes tested on 3D MeWo static culture induced partial spheroid disaggregation after 24 h of culture, with almost half of the cells dead. (unibas.it)
  • Many culturing strategies are based on the addition of feeder or accessory cells, which need to be removed prior to the clinical application of the final NK cell product. (frontiersin.org)
  • It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. (wikipedia.org)
  • It can reduce the sulfation of cell surface heparan sulfate proteoglycan (HSPG) and inhibit various growth factor receptor-mediated signaling pathways. (oncotarget.com)
  • Interestingly, NRAGE was able to overcome the protection afforded to 32D cells by the exogenous expression of human Bcl-2. (uni-wuerzburg.de)
  • The ethanolic extract of T. camphoratus (TCEE) which contains abundant bioactive compounds including triterpenoids and polysaccharides also has antitumor effects in various human cancer cell lines. (hindawi.com)
  • Against the human oral cancer cells. (biomedcentral.com)
  • Ms Portia Raphela-Choma from the University of Johannesburg elaborated on the cytotoxicity effects of medicinal plant-derived compounds on human cancerous cell lines. (acgt.co.za)
  • Apigenin induces autophagic cell death in human papillary thyroid carcinoma BCPAP cells. (spandidos-publications.com)
  • 1987. The effect of electrically generated silver ions on human cells. (cdc.gov)