In the current study, we utilized modified Spirulina maxima pectin nanoparticles as a supplement to improve porcine in vitro maturation medium. (mdpi.com)
This study may be an important supplement for the mechanism of oocyte meiotic resumption and provide new diagnostic targets and treatment clues for infertility patients with oocyte maturation disorder. (cambridge.org)
Female1
The low maturation rate of oocytes is an important reason for female infertility and failure of assisted pregnancy. (cambridge.org)
Article1
A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Embryo6
7 A female embryo after 18 weeks contains four phases of FGCs, i.e. mitotic, retinoid acid-responsive, meiotic prophase and oogenetic FGCs, and a male embryo after 9 weeks contains two phases of FGCs, i.e. mitotic, and mitotic-arrest FGCs. (nature.com)
Embryo transfer entails many procedures and techniques, of which embryo freezing is an important component in bovine embryo transfer. (go.jp)
Embryo freezing techniques have been developed over the last 40 years, allowing practical availability, and have become essential for cattle reproduction management under field conditions. (go.jp)
Our proof-of-concept experiments show that replacement of the entire cytoplasm of oocytes from a sensitive mouse strain overcomes massive embryo developmental arrest characteristic of non-manipulated oocytes. (elifesciences.org)
These results demonstrate that 2 ISO improves the quality of porcine oocytes by protecting them against oxidative stress during IVM and enhances subsequent embryo development in vitro. (bvsalud.org)
Several potential options are available to preserve fertility in patients who face premature ovarian failure, including immature or mature oocyte and embryo cryopreservation. (cambridge.org)
MtDNA6
It also discusses the effects of ooplasmic transfer on oocytes and embryos, and mtDNA. (fertiligy.com)
During ovulation, a newly fertilized oocyte carries mtDNA from both parents. (fertiligy.com)
The oocyte has two mtDNA haploplasmy states - mitochondrial heteroplasmy in the donor, and mitochondrial homoplasmy in the recipient oocyte. (fertiligy.com)
This pre-clinical model demonstrates the high efficiency and potential of the MST technique, not only to prevent the transmission of mtDNA mutations, but also as a new potential treatment for patients with certain forms of infertility refractory to current clinical strategies. (elifesciences.org)
We performed mitochondrial DNA Next Generation Sequencing and determined that one mtDNA variant harbored by EPCs was present in oocytes, preimplantation embryos and somatic tissues over three generations in our mini-pig model indicating the potential germline origin of EPCs. (oncotarget.com)
The quality of mitochondria and the numbers of copies of its genome, mitochondrial DNA (mtDNA), in oocytes are beginning to be considered, along with other factors, as indicators of oocyte quality, especially in the context of aging [ 5 - 11 ]. (oncotarget.com)
Developmental competence2
Here, we explore the utility of the maternal spindle transfer (MST) technique as a reproductive approach to enhance oocyte developmental competence. (elifesciences.org)
This study investigated the effect of the flavonoid-based compound isorhamnetin (ISO) on maturation and developmental competence in oxidative stress-exposed porcine oocytes in vitro. (bvsalud.org)
Sperm3
PACRG is a protein found in the mitochondrial sheath of the oocyte prior to fertilization and at the tail of the sperm after exposure to oocyte extract. (fertiligy.com)
Fetal germ cells (FGCs) are embryonic precursors of mature gametes, i.e. sperm or oocytes. (nature.com)
Male-factor infertility can be overcome by a technique called intracytoplasmic sperm injection (ICSI), which directly injects a sperm into an oocyte, and is one of the most widely used treatments in the fertility clinic [ 3 ]. (oncotarget.com)
Oxidative stress1
Treatment with 2 µM ISO (2 ISO) increases the developmental rate of oxidative stress-exposed porcine oocytes during in vitro maturation (IVM). (bvsalud.org)
Embryos4
Ooplasm donation is a technique where donor oocytes are used to make embryos. (fertiligy.com)
The direct transfer methods of frozen-thawed, in vivo- derived, and in vitro- produced (IVF) bovine embryos using 1.5 M ethylene glycol (EG) with or without sucrose (SUC) are used widely under on-farm conditions, not only in Japan but also globally. (go.jp)
The pregnancy rate with direct transfer of frozen-thawed bovine embryos in either EG or GLY-SUC has been found to not differ from conventional freezing with GLY and traditional dilution techniques. (go.jp)
The developmental potential of early embryos is mainly dictated by the quality of the oocyte. (elifesciences.org)
Porcine2
Therefore, we propose that ISO is a useful supplement for IVM of porcine oocytes. (bvsalud.org)
The presence of Zp1 , Zp2 , Zp3 and Nobox were not detected, indicating that porcine EPCs are not indicative of mature primordial oocytes. (oncotarget.com)
Fertilization3
It controls meiotic division, fertilization, and the activation of the embryonic genome. (fertiligy.com)
Medical procedures known as in vitro fertilization (IVF) help many individuals experiencing infertility to have children. (elifesciences.org)
On the other hand, treatment for female-factor infertility is often restricted to in vitro fertilization, since ICSI does not appear to be an effective treatment for poor quality oocytes [ 4 ]. (oncotarget.com)
Ovarian2
Therefore, this review aims to summarize the achievements of ovary grafting and the potential techniques that have been developed to improve ovarian graft survival. (cambridge.org)
Female-factor infertility can be caused by poor oocyte quality and depleted ovarian reserves. (oncotarget.com)
Cells3
Using this technique, it is possible to generate human egg cells for IVF that have the genetic material from the intended mother without the defects in the cytoplasm that may be responsible for infertility. (elifesciences.org)
applied MST to infertile female mice and found that the technique could permanently correct deficiencies in the cytoplasms of poor quality egg cells, allowing the mice to give birth to healthy offspring. (elifesciences.org)
These findings open up the possibility of developing new treatments for infertility caused by problems with egg cells, so experiments involving human egg cells are now being performed to evaluate the safety and effectiveness of the technique. (elifesciences.org)
Markers2
Besides, our previous study showed that IL13RA2 (also known as CD213α2) and PECAM1 (also known as CD31) were specific surface markers of meiotic prophase FGCs and oogenetic FGCs, respectively. (nature.com)
Expression of the pluripotent markers Sox2 and Oct4 , and the primitive germ cell markers Blimp1 and Stella were not detected. (oncotarget.com)
Generate1
Similarly, the oocytes' actin networks generate pulling forces near the ooplasm-yellow granule interface. (fertiligy.com)
Quality3
Without ooplasmic regulators, oocytes can be rendered of low quality. (fertiligy.com)
Coupled to the greater precursor supply is increased physiological germline cell death, which is required to improve oocyte quality in older mothers. (bvsalud.org)
An increasing number of women are delaying childbirth, and, since oocyte quality declines dramatically after 35 years of age [ 1 , 2 ], more women are requiring assisted reproductive treatments. (oncotarget.com)
Numbers1
This dogma states that female mammals are born with finite numbers of oocytes that decline throughout postnatal life. (cambridge.org)
Human1
Finally, using an in vitro culture system of human FGCs, we showed that the BMP signaling pathway promoted the cell proliferation of FGCs, and regulated the WNT signaling pathway by orchestrating the chromatin accessibility of its ligand genes. (nature.com)
Cell2
To address these questions, we cultured 7-week male FGCs from testis in vitro with or without its antagonist LDN-193189 2HCl (LDN), and analyzed the transcriptional and epigenetic differences at single-cell level between these two conditions. (nature.com)
Injection of aspirin (an inhibitor of cyclooxygenase (COX)) after blood-feeding (BF) inhibited oogenesis by preventing nurse cell dumping into a growing oocyte. (bvsalud.org)