Meiosis i mouse oocytes spindle in vitro oocyte maturation techniques. Medical search. Frequent questions

Meiosis then proceeds to second metaphase, where it pauses again until fertilization. (wikipedia.org)
We found that oocytes deprotected from their surrounding layer of follicular cells and expressing the epidermal growth factor (EGF) receptor (EGFR) and the Grb7 adaptor undergo accelerated prophase I to metaphase II meiosis progression upon stimulation by EGF. (bvsalud.org)
In this study, by whole-exome sequencing (WES), we identified homozygous and compound heterozygous MAD2L1BP variants in three families with female patients diagnosed with primary infertility owing to oocyte metaphase I (MI) arrest. (bvsalud.org)
Significant reduction of germinal vesicle (GV) and MII oocytes and significant increase of metaphase chromosome misalignment and premature chromatid segregation after meiotic maturation of oocytes, similar to human, presumably contribute to the decline in aged KM mice. (cambridge.org)
JNK2 was associated with the spindles especially the spindle poles and cytoplasmic microtubule organizing centers at prometaphase I, metaphase I, and metaphase II stages. (cambridge.org)

In addition, we determined that oocytes surrounded by their follicular cells expressing EGFR-Grb7 exhibit normal meiotic resumption. (bvsalud.org)
This study may be an important supplement for the mechanism of oocyte meiotic resumption and provide new diagnostic targets and treatment clues for infertility patients with oocyte maturation disorder. (cambridge.org)

As follicles grow, they acquire receptors for luteinizing hormone, a pituitary hormone that reinitiates meiosis in the oocyte and causes ovulation of a fertilizable egg. (wikipedia.org)
These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)

The renewal of ovarian follicles from germline stem cells (originating from bone marrow and peripheral blood) has been reported in the postnatal mouse ovary. (wikipedia.org)
Mammalian ovaries are endowed with a huge number of small oocytes (primordial oocytes) in primordial follicles. (go.jp)
The mean number of ovarian follicles, ovarian germinal vesicle oocytes and ovulated MII oocytes decreased significantly with maternal age. (cambridge.org)

Oogonia enter meiosis during embryonic development, becoming oocytes. (wikipedia.org)
Understanding the biological functions of miRNAs in frozen maturated oocytes may provide a better understanding of embryonic development and a comparison of fertility conservation in female mammals. (bvsalud.org)

This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
Intracytoplasmic sperm injection ( ICSI / ˈ ɪ k s i / IK -see ) is an in vitro fertilization (IVF) procedure in which a single sperm cell is injected directly into the cytoplasm of an egg . (wikipedia.org)

Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)
In this review, we summarize the results regarding the activation of primordial oocytes in neonatal and adult ovaries of different species and propose a model in which ovaries of neonatal mammals contain a mixed population of both quiescent and activated primordial oocytes, while almost all primordial oocytes are quiescent in adult females. (go.jp)
FOXO3 is considered one of the molecules responsible for the dormancy of primordial oocytes in adult ovaries. (go.jp)
At the end of 15 days, five mice in each group were euthanized and the ovaries were analyzed for follicular morphometry and granulosa cell count. (biomedcentral.com)

Mammalian oocytes are maintained in meiotic prophase arrest for a very long time-months in mice, years in humans. (wikipedia.org)
It is well known that c-Jun N-terminal kinase (JNK) plays pivotal roles in various mitotic events, but its function in mammalian oocyte meiosis remains unknown. (cambridge.org)

SECM technique may be a valuable tool for accurately assessing the quality of embryos and thereby contribute to improving outcomes associated with assisted reproduction, including human in vitro fertilization. (bioone.org)
Usage of melatonin during in vitro fertilization (IVF) procedures has been found to improve oocyte quality, survival, and fecundity. (jrhm.org)
Round spermatid injection (ROSI) is a technique of assisted reproduction whereby a round spermatid is injected into oocyte cytoplasm in order to achieve fertilization. (wikipedia.org)
To ascertain whether the Kunming (KM) mouse is an available model for age-related decline in female fertility in human or not, oocytes from young (6-8 weeks), middle-aged (9 months) and aged (12 months) female mice were compared with respect to number of oocytes, frequency of in-vitro maturation (IVM) and in-vitro fertilization (IVF), and meiotic chromosome segregation and alignment. (cambridge.org)

Oogenesis consists of several sub-processes: oocytogenesis, ootidogenesis, and finally maturation to form an ovum (oogenesis proper). (wikipedia.org)
Second Polar Body (Discarded afterward) + Ovum Oocyte meiosis, important to all animal life cycles yet unlike all other instances of animal cell division, occurs completely without the aid of spindle-coordinating centrosomes. (wikipedia.org)
Diagram showing the reduction in number of the chromosomes in the process of maturation of the ovum . (wikipedia.org)

The germinal vesicle breakdown (GVBD) is a landmark event of oocyte maturation. (cambridge.org)
In our previous studies, we found that zona pellucida 3 (ZP3) was strongly concentrated in the nuclear region of germinal vesicle (GV) oocytes and interacted with aryl hydrocarbon receptor-interacting protein-like 1 (AIPL1) and lamin A to promote GVBD. (cambridge.org)

The granulosa cells produce a small molecule, cyclic GMP, that diffuses into the oocyte through the gap junctions. (wikipedia.org)
Because the granulosa cells and oocyte are connected by gap junctions, cyclic GMP also decreases in the oocyte, causing meiosis to resume. (wikipedia.org)
Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)

We think that primordial oocytes in adult mammals are different from those in neonatal mammals. (go.jp)
The dormancy of primordial oocytes may be required to reserve the non-growing oocyte pool for the long reproductive life in mammals. (go.jp)
However, to the best of our knowledge, no previous studies have evaluated the role of MTSPs in female meiosis in mammals. (cambridge.org)

Further, melatonin and its receptors play a significant role in antioxidant defense mechanism of ovary for follicular growth and maturation. (jrhm.org)
Xenopus oocytes are encompassed by a layer of follicular cells that contribute to oocyte growth and meiosis in relation to oocyte maturation. (bvsalud.org)
However, the effects of the interaction between follicular cells and the oocyte surface on meiotic processes are unclear. (bvsalud.org)
Here, we investigated Xenopus follicular cell function using oocyte signaling and heterologous-expressing capabilities. (bvsalud.org)
Furthermore, an O-GlcNAcylation increase (by inhibition of O-GlcNAcase), the glycosidase that removes O-GlcNAc moieties, or decrease (by inhibition of OGT) amplifies oocyte spindle defects when follicular cells are absent highlighting a control of the meiotic spindle by the OGT-O-GlcNAcase duo. (bvsalud.org)
In summary, our study provides further insight into the role of the follicular cell layer in oocyte meiosis progression. (bvsalud.org)

In this paper, we describe a scanning electrochemical microscopy (SECM) technique that is a non-invasive and sensitive method for measuring oxygen consumption by individual embryos. (bioone.org)
MicroRNAs (miRNAs) are small non-encoding RNAs that actively regulate biological and physiological processes, and play an important role in regulating gene expression in all cells, especially in most animal cells, including oocytes and embryos. (bvsalud.org)
Although assisted reproductive techniques (ART) have helped to solve many infertility problems, they cause changes in the expression of miRNA and genes in oocytes and preimplantation embryos, and the effect of these changes on the future of offspring is unknown, and has caused concerns. (bvsalud.org)
This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal uterus. (wikipedia.org)
If all requirements for round spermatid selection and injection are successfully met, the injected oocytes develop to early embryos and can be transferred to the mother's uterus to produce pregnancy. (wikipedia.org)

Injection of myc-tagged JNK2α1 mRNA into oocytes also revealed its localization on spindle poles. (cambridge.org)
It was found that FIGNL1, a member of MTSPs, was predominantly expressed in mouse oocytes and distributed at the spindle poles during meiosis in the present study. (cambridge.org)

Once the kinetochores of chromosomes are correctly attached to bipolar spindles and the SAC is satisfied, the MAD2L1BP, best known as p31comet, binds mitosis arrest deficient 2 (MAD2) and recruits the AAA+-ATPase TRIP13 to disassemble the mitotic checkpoint complex (MCC), leading to the cell-cycle progression. (bvsalud.org)
Works in various organisms have revealed that the kinase is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability. (lookformedical.com)

The succeeding phase of ootidogenesis occurs when the primary oocyte develops into an ootid. (wikipedia.org)

cRNA microinjection of full-length or truncated MAD2L1BP uncovered their discordant roles in driving the extrusion of polar body 1 (PB1) in mouse oocytes. (bvsalud.org)
Furthermore, the patient's oocytes carrying the mutated MAD2L1BP resumed polar body extrusion (PBE) when rescued by microinjection of full-length MAD2L1BP cRNAs. (bvsalud.org)

In terms of insemination, ICSI needs only one sperm cell per oocyte , while IVF needs 50,000-100,000. (wikipedia.org)

From one single oogonium, only one mature oocyte will rise, with 3 other cells called polar bodies. (wikipedia.org)

At puberty the number of oocytes decreases even more to reach about 60,000 to 80,000 per ovary, and only about 500 mature oocytes will be produced during a woman's life, the others will undergo atresia(degeneration). (wikipedia.org)
The aim of this study was to assess the effects of brief, intense exposure to tobacco smoke on granulosa cell number, oocyte growth, and follicle size during puberty in female Swiss mice. (biomedcentral.com)
Moreover, brief exposure during puberty may induce silent oocyte disruption, which could in turn lead to decreased fecundity rates. (biomedcentral.com)

The spindle assembly checkpoint (SAC) is an intricate surveillance mechanism that ensures accurate segregation of chromosomes throughout cell cycles. (bvsalud.org)

Oogenesis starts with the process of developing primary oocytes, which occurs via the transformation of oogonia into primary oocytes, a process called oocytogenesis. (wikipedia.org)
It is commonly believed that, when oocytogenesis is complete, no additional primary oocytes are created, in contrast to the male process of spermatogenesis, where gametocytes are continuously created. (wikipedia.org)

Human oocyte maturation arrest represents one of the severe conditions for female patients with primary infertility. (bvsalud.org)
Together, our studies identified and characterized novel biallelic variants in MAD2L1BP responsible for human oocyte maturation arrest at MI, and thus prompted new therapeutic avenues for curing female primary infertility. (bvsalud.org)
The low maturation rate of oocytes is an important reason for female infertility and failure of assisted pregnancy. (cambridge.org)

Therefore, in future, melatonin can be implicated as preferable therapeutic especially in IVF and assisted reproductive techniques. (jrhm.org)

However, as the oocyte grows, these proteins are synthesized, and meiotic arrest becomes dependent on cyclic AMP. (wikipedia.org)
Maintenance of meiotic arrest also depends on the presence of a multilayered complex of cells, known as a follicle, that surrounds the oocyte. (wikipedia.org)
In the oocyte, cyclic GMP prevents the breakdown of cyclic AMP by the phosphodiesterase PDE3, and thus maintains meiotic arrest. (wikipedia.org)

These quiescent primordial oocytes are activated, perhaps by certain mechanisms involving the interaction between stimulatory and inhibitory factors, to enter the growth phase. (go.jp)
When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)

In vivo development was assessed after transfer of injected oocytes to the oviducts of recipient mares. (bioone.org)
This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)

An aurora kinase that localizes to the CENTROSOME during MITOSIS and is involved in centrosome regulation and formation of the MITOTIC SPINDLE. (lookformedical.com)

Little is known about the mechanism regulating the activation of primordial oocytes. (go.jp)

The study suggests that KM mouse exhibits an age-related decline in female fertility. (cambridge.org)

The mean number of pups born per mouse decreased significantly from the young to the middle-aged and the aged mice. (cambridge.org)
The rate of IVM in oocytes from young mice (73.9%) was less significantly than that in oocytes from middle-aged and aged mice (86.1% and 84.4%, respectively). (cambridge.org)
Immunocytochemical analysis showed that ageing caused a significantly higher rate (49.3%) of chromosome misalignment than that (15.7%) of the young mice. (cambridge.org)
AIPL1 was also proved to accumulate in the GV region of oocytes, and ZP3 deletion can significantly inhibit the aggregation of AIPL1 in the nuclear region. (cambridge.org)

Those transformed cells form tumours when implanted in immunodeficient mice, indicating that the kinase is an oncogene. (lookformedical.com)
When implanted subcutaneously in mice with intradermally transplanted tumours, the device led to significant antitumour effects by irradiating for 10 d at approximately 1,000-fold lower intensity than conventional PDT approaches. (regenerativemedicine.net)

[2] The microinjection procedure for ROSI also differs slightly from that of ICSI, since additional stimuli are needed to ensure proper oocyte activation after spermatid injection. (wikipedia.org)

Genome-wide association for testis weight in the diversity outbred mouse population. (middlebury.edu)

It is developed from the primary oocyte by maturation. (wikipedia.org)

Folliculated oocytes can be forced to adopt an abnormal phenotype and exclusive Grb7 Y338 and Y188 phosphorylation instead of O-GlcNAcylation under integrin activation. (bvsalud.org)

The expression of miRNAs at the right time and place is crucial for the oocyte's maturation and the embryo's subsequent development. (bvsalud.org)

Thus, we investigated male mouse (m) PRL protein and mRNA expression in spermatozoa at various differentiation stages in the testes. (go.jp)

Following vaginal opening, the mice were checked for estrous cyclicity by vaginal cytology at 9:00-10:00 am daily throughout the study. (biomedcentral.com)

These oocytes are protected from abnormal meiotic spindle formation through the recruitment of O-GlcNAcylated Grb7, and OGT (O-GlcNAc transferase), the enzyme responsible for O-GlcNAcylation processes, in the integrin ß1-EGFR complex. (bvsalud.org)

In those men in whom spermatogenesis is blocked at the stage of round spermatids, in which meiosis has already been completed, these round cells can successfully fertilize oocytes after being injected into their cytoplasm. (wikipedia.org)

This unusual maturation unravels atypical spindle formation but is rescued by inhibiting integrin ß1 or Grb7 binding to the EGFR. (bvsalud.org)

Ten female Swiss mice aged 35 days were exposed to tobacco smoke from 3R4F reference research cigarettes. (biomedcentral.com)
Twenty female Swiss mice were obtained after birth. (biomedcentral.com)

Meiosis begins with DNA replication and meiotic crossing over. (wikipedia.org)

Even though many technical aspects of ROSI are similar to those of ICSI, there are also significant differences between both techniques. (wikipedia.org)

Anatomy15

Organisms3

Diseases4

Chemicals and Drugs5

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Phenomena and Processes17

Information Science1

Metaphase5

Meiotic resumption2

Ovulation2

Follicles3

Embryonic development2

Intracytoplasmic Sperm Inj2

Ovaries4

Mammalian2

Fertilization4

Ovum3

Germinal vesicle2

Granulosa cells3

Mammals3

Follicular6

Embryos5

Poles2

Bipolar2

Ootidogenesis1

Polar Body2

Sperm1

Oogonium1

Puberty3

Checkpoint1

Oocytogenesis2

Infertility3

Reproductive techniques1

Arrest3

Growth2

Vivo2

Mitotic1

Mechanism1

Suggests1

Significantly4

Tumours2

Injection1

Testis1

Primary1

Activation1

Development1

MRNA1

Study1

Processes1

Stage1

Formation1

Female2

Begins1

Significant1