MPN-238 Single-Cell RNA Profiling of Myelofibrosis Patients Reveals Pelabresib-Induced Decrease of Megakaryocytic Progenitors and Normalization of CD4+ T Cells in Peripheral Blood. (ox.ac.uk)
RESULTS: Analysis of CD34+ HSPCs at baseline demonstrated increased numbers of megakaryocytic, neutrophilic, and erythroid progenitors in myelofibrosis patients compared with HDs and a significant reduction of myeloid and B-cell lineage progenitors. (ox.ac.uk)
Pelabresib as monotherapy and combined with ruxolitinib led to significant reduction of megakaryocytic, neutrophilic, and erythroid progenitors compared with baseline. (ox.ac.uk)
Shigeo Kariyone Megakaryocytopoiesis in polycythemia vera (PV) was characterized by in vitro growth of marrow megakaryocytic progenitors (CFU-Meg) and quantitation of megakaryocyte numbers in marrow biopsy specimens in 14 patients with PV. (karger.com)
Hyperplasia3
and hematopoietic cellularity of 80% with megakaryocytic hyperplasia demonstrating normal bone marrow function with Clinical Presentations increased platelet precursor cells. (cdc.gov)
7 Pathologically, MF is characterized by thickening and distortion of bony trabeculae, deposition of reticulin and collagen fibers, and megakaryocytic hyperplasia with atypical features. (haematologica.org)
Essential Thrombocythemia Essential thrombocythemia (ET) is a myeloproliferative neoplasm characterized by an increased platelet count, megakaryocytic hyperplasia, and a hemorrhagic or microvascular vasospastic tendency. (msdmanuals.com)
Differentiation of human1
Platelet-derived growth factor (PDGF) is involved in megakaryocytopoiesis and is secreted into the culture medium during megakaryocytic differentiation of human leukemic cells. (edu.hk)
Hematopoietic1
This phenomenon occurs in otherwise unmanipulated murine marrow in vivo, resulting in circulating platelets that bear membrane from non-megakaryocytic hematopoietic donors. (elifesciences.org)
Myeloid2
In myelofibrosis patients at baseline, larger spleen volume was observed in patients with lower numbers of CD4+ T cells and increased numbers of megakaryocytic and myeloid CSF3R+ cells. (ox.ac.uk)
Bone marrow failure syndrome (BMFS) is a group of disorders that may manifest as single cytopenia (eg, erythroid, myeloid, or megakaryocytic) or as pancytopenia. (medscape.com)
Platelet2
A clear and unambiguous criteria for the identification of megakaryocytic cells is described based on the high expression of platelet glycoprotein (e.g. (edu.hk)
FGF-6 is expressed in leukemia cell lines with platelet megakaryocytic differentiation potential. (peprotech.com)
K5622
These mRNAs were detected in human leukemia cells, CMK, K562 and HEL, which have megakaryocyticphenotype or the potency to differentiate into megakaryocytic lineage. (elsevierpure.com)
Western blot analysis revealed that conditioned medium from 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated, megakaryocytic differentiating K562 cells upregulated DAB2 expression. (edu.hk)
Quantitation1
Conclusions: This finding demonstrates that without careful definition, the quantitation of megakaryocytic cells will be inappropriately high. (edu.hk)
Megakaryocytes1
We identified a new morphological parameter, defined as Megakaryocytic Activation (M-ACT) based on the coexistence of megakaryocytic emperipolesis, megakaryocytes (MK) clusters formation and evidence of arrangement of collagen fibers around the perimeter of MK. (unicatt.it)
Dive into the research topics of 'Initial characterization of a human megakaryocyticleukemiacell line, EST-IU. (wustl.edu)
Lineage3
CONTEXT: Abnormal differentiation of the megakaryocytic lineage and overproduction of proinflammatory cytokines, resulting in bone marrow fibrosis, anemia, symptoms, extramedullary hematopoiesis, and often hepatosplenomegaly, are key characteristics of myelofibrosis. (ox.ac.uk)
Individual patients exhibited reduction in natural killer cells and CD16+ monocytes as well as elevated megakaryocytic lineage cells. (ox.ac.uk)
Pelabresib as monotherapy and combined with ruxolitinib increased the proportion of CD4+ T cells, and, importantly, reduced megakaryocytic lineage cells in both treatment-naïve and ruxolitinib-relapsed/refractory patients. (ox.ac.uk)
Bone2
For that purpose, we studied megakaryocytic development of HPC, obtained from patients with hematological malignancies in complete remission or solid tumors without bone marrow involvement, under the influence of megakaryocyte growth and development factor (MGDF, a pegylated, truncated molecule related to the endogenous Mpl ligand). (amsterdamumc.org)
Several months later, in March of 2017, a bone marrow biopsy revealed that Joel also had AML (Acute Megakaryocytic Leukemia: AML-M7). (stbaldricks.org)
Human2
Using ultrastructural and confocal laser scanning microscopic (CLSM) image analysis, we observed that treatment of Dami cells, a human megakaryocyticcell line, with phorbol myristate acetate (PMA) induces the accumulation of PAI-1 and Vn in intracellular storage vacuoles that contain other alpha-granule proteins such as von Willebrand factor. (mcmaster.ca)
Human models of NUP98-KDM5A megakaryocyticleukemia in mice contribute to uncovering new biomarkers and therapeutic vulnerabilities. (atlasgeneticsoncology.org)
Cells2
These cells were found to have typical monocytic morphology but no megakaryocytic characteristics. (edu.hk)
DAB2 induction and megakaryocytic differentiation was abrogated when cells were co-treated with the PDGF receptor inhibitor STI571 or when the conditioned medium was derived from TPA-plus STI571-treated cells. (edu.hk)
This study shows for the first time that PDGF autocrine signaling regulates DAB2 expression during megakaryocytic differentiation. (edu.hk)
Patients1
DS patients may also exhibit congenital heart disease between 40 and 50% of the cases an increased risk for developing Alzheimer's disease, acute megakaryocyticleukemia, Hirschsprung's disease and duodenal atresia. (bvsalud.org)
Leukemia5
These mRNAs were detected in human leukemia cells, CMK, K562 and HEL, which have megakaryocyticphenotype or the potency to differentiate into megakaryocytic lineage. (elsevierpure.com)
This translocation has been associated with acute megakaryocyticleukemia. (wikipedia.org)
Bone marrow failure syndrome (BMFS) is a group of disorders that may manifest as single cytopenia (eg, erythroid, myeloid, or megakaryocytic) or as pancytopenia. (medscape.com)
The transcription factors that control lineage specification of haematopoietic stem cells (HSCs) have been well described for the myeloid and lymphoid lineages, whereas transcriptional control of erythroid (E) and megakaryocytic (Mk) fate is less understood. (ox.ac.uk)
FOG-1-deficient HSCs and preMegEs, the latter normally bipotent for the Mk and E lineages, underwent myeloid transcriptional reprogramming, and formed myeloid, but not erythroid and megakaryocytic cells in vitro. (ox.ac.uk)
Identification of Flt3+ lympho-myeloid stem cells lacking erythro-megakaryocytic potential a revised road map for adult blood lineage commitment. (ox.ac.uk)
Additionally, mutated immature myeloid progenitors showed a megakaryocytic-erythroid lineage bias, also as previous mouse studies have found. (genomeweb.com)
Specific diseases represented among specimens tested include various examples of non-Hodgkin's lymphoma, reactive lymphoid proliferations, lymphoproliferative disorders, myeloid, lymphoid, monocytic and megakaryocytic leukemias, acquired and primary immunodeficiencies and granulocyte disorders. (amc.edu)
Erythroid and megakaryocytic1
Cells in the Lin-Sca-1+c-kit+ HSC compartment coexpressing high levels of the tyrosine kinase receptor Flt3 sustain granulocyte, monocyte, and B and T cell potentials but in contrast to Lin-Sca-1+c-kit+Flt3- HSCs fail to produce significant erythroid and megakaryocytic progeny. (ox.ac.uk)
Precursors1
In spleens of 6- to 7-week-old embryos hemocytoblasts, proerythroblasts and megakaryocytic precursors were identified, located. (karger.com)
Differentiation3
The goal of this study was to define the relative sensitivities of commonly used stains for markers of megakaryocytic differentiation. (medscape.com)
A sensitive and reliable approach to the identification of megakaryocytic differentiation in leukemic blasts can thus be the first step in achieving optimal outcomes for pediatric patients with AMKL. (medscape.com)
The diagnosis of AMKL is established by the presence of 20% or more blasts in the bone marrow or peripheral blood, of which at least 50% must demonstrate immunophenotypic evidence of megakaryocytic differentiation either by flow cytometry (FC) and/or immunohistochemistry (IHC). (medscape.com)
Myeloproliferative2
Bone marrow megakaryocytic activation predicts fibrotic evolution of Philadelphia-negative myeloproliferative neoplasms. (cdc.gov)
Essential thrombocythemia (ET) is a myeloproliferative neoplasm characterized by an increased platelet count, megakaryocytic hyperplasia, and a hemorrhagic or microvascular vasospastic tendency. (msdmanuals.com)
Monocytic1
It is often difficult to distinguish blast cells of myelocytic, monocytic, or megakaryocytic lineages from lymphoblasts. (sysmex-europe.com)
Cell lines1
Well-established molecular and genetic evidence, along with clinical observations, suggests that a relationship exists between the erythrocytic and megakaryocytic cell lines. (shengsci.com)