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  • anaphase
  • E) Megakaryocyte in anaphase A was stained with DAPI (a), anti-α-tubulin antibody (b), anticentromere antibody (c), or all three (d). (nih.gov)
  • membrane
  • Studies of cultured megakaryocytes will include evaluation of granule membrane and soluble proteins using fluorescent antibodies and immunoelectron microscopy and comparison of RNA and protein expression patterns between normal and patient cells. (clinicaltrials.gov)
  • receptor
  • Indeed, we demonstrate that megakaryocytes express the major candidates to mediate Store-Operated Calcium Entry, stromal interaction molecule 1, Orai1 and canonical transient receptor potential 1, which are activated upon either pharmacological or physiological depletion of the intracellular calcium pool. (haematologica.org)
  • nucleus
  • As a result, the nucleus of the megakaryocyte can become very large and lobulated, which, under a light microscope, can give the false impression that there are several nuclei. (wikipedia.org)
  • As a result, the nucleus of the megakaryocyte may contain up to 32 copies of the normal complement of DNA in a human cell. (sciencephoto.com)
  • Anticentriole antibody (Fig. 3 A, c) and anti-α-tubulin antibody (Fig. 3 A, b) stainings confirmed that multiple mitotic spindle poles were formed and assembled outside the nucleus in a megakaryocyte in prophase, as described above. (nih.gov)
  • nuclear
  • 1 The transition from proliferating to differentiating megakaryocytes is accompanied by loss of nuclear-associated acetylcholinesterase activity. (ahajournals.org)
  • With each replicative event, the entire DNA content is duplicated such that megakaryocytes have multiples of a normal diploid DNA content (i.e., 4N, 8N, 16N, 32N, etc., where 2N is the normal nuclear DNA content of a cell in G 0 /G 1 phase of the cell cycle). (rupress.org)
  • The 5q-syndrome is characterized by macrocytic anemia, often a moderate thrombocytosis, erythroblastopenia, megakaryocyte hyperplasia with nuclear hypolobation, and an isolated interstitial deletion of chromosome 5. (wikipedia.org)
  • HUMAN
  • Here we show that adenosine diphosphate elicits, in human megakaryocytes, an increase in cytosolic calcium concentration, followed by a plateau, which is lowered in absence of extracellular calcium, suggesting the involvement of Store-Operated Calcium Entry. (haematologica.org)
  • These findings provide the first evidence that both calcium mobilization from intracellular stores and extracellular calcium entry specifically regulate human megakaryocyte functions. (haematologica.org)
  • primary
  • A number of primary megakaryocytes treated with TPO were stained with DAPI, anti-α-tubulin antibody, anti-γ-tubulin antibody, anticentriole antibody, or anticentromere antibody. (nih.gov)
  • colony
  • Megakaryocytes can also be grown in diffusion chambers although with a lower efficiency and smaller colony size than in plasma clot cultures. (springer.com)