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  • Moreover
  • Moreover, the cell line is amenable to biochemical and transcriptional analyses, as we report for GpVI, a direct target of SCL. (ox.ac.uk)
  • polycythemia vera
  • Adamson JW, Fialkow PJ, Murphy S, Prchal JF, and Steinmann L. Polycythemia vera: stem cell and probable clonal origin of the disease. (springer.com)
  • Asimakopoulos F, Hinshelwood S, Gilbert J, Delibrias C, Gottgens B, Fearon D, and Green A. The gene encoding hematopoietic cell phophatase (SHP-1) is structurally and transcriptionally intact in polycythemia vera. (springer.com)
  • Chiba S, Takahashi T, Takeshita K, Minowada J, Yazaki Y, Ruddle FH, and Hirai H. Selective expression of mRNA coding for the truncated form of erythropoietin receptor in hematopoietic cells and its decrease in patients with polycythemia vera. (springer.com)
  • Polycythemia vera is a clonal disorder characterized by the overproduction of mature red blood cells. (clevelandclinicmeded.com)
  • human
  • We are using hematopoietic stem cells and human embryonic stem cells to better understand Acute Megakaryoblastic Leukemia. (yale.edu)
  • When the Human Erythroleukemia (HEL) cell line is induced to differentiate with TPA, overexpression of MKL1 results in an increased number of megakaryocytes with a concurrent increase in ploidy. (yale.edu)
  • Recombinant human megakaryocyte growth and development factor stimulates throm-bocytopoiesis in normal nonhuman primates. (springer.com)
  • Thrombopoietic effects of pegylated recombinant human megakaryocyte growth and development factor (PEG-rHuMGDF) in patients with advanced cancer. (springer.com)
  • The ED 50 as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found to be ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10 6 units/mg. (abcam.com)
  • Cytospin preparation after 7-day culture of the novel human megakaryocyte progenitor (MegP) in liquid medium (May-Giemsa staining). (bloodjournal.org)
  • CREB is overexpressed and constitutively phosphorylated in a number of forms of human cancer, including acute myeloid leukemia (AML) and non-small cell lung cancer, and appears to play a direct role in disease pathogenesis and prognosis. (aacrjournals.org)
  • mutations
  • Although transcription factors are not often directly modified by mutations in cancer cells, they frequently become activated constitutively through mutations affecting "upstream" pathways. (aacrjournals.org)
  • Growth
  • 3 This mutation substitutes phenylalanine for valine at position 617 in the JH2 domain (Val617Phe, V617F) of exon 14, leading to constitutive activation of the JAK -STAT and other pathways resulting in uncontrolled cell growth. (clevelandclinicmeded.com)
  • Although dramatic advances have been made through the use of kinase inhibitors such as Bcr-Abl inhibitors in chronic myeloid leukemia (CML) or epidermal growth factor receptor (EGFR) kinase inhibitors in non-small cell lung cancer, a number of hurdles have arisen. (aacrjournals.org)
  • Growth factors and cytokines can trigger cell-cycle progression and other cellular events through the rapid and transient activation of transcription factors that regulate the expression of genes such as cyclins. (aacrjournals.org)
  • Both have been shown to be potent stimulators of megakaryocyte growth and platelet production and are biologically active in reducing the thrombocytopenia of nonmyeloablative chemotherapy. (bloodjournal.org)
  • pathways
  • For example, many kinase and cell-surface receptor-triggered pathways converge on transcription factors such as NF-κB or STAT family members. (aacrjournals.org)
  • Since normal cells are relatively insensitive to inhibition of these proteins due to redundancies in normal signaling pathways, inhibitors of these transcription factors hold the potential for having a very high therapeutic index. (aacrjournals.org)
  • Blood Cells
  • This project aims to study iron deficiency anemia as a model of benign elevated platelet and reduced red blood cells (RBCs), with a novel focus on the molecular mechanisms of the MEP biphenotypic fate decision. (aabb.org)
  • transcriptional
  • Moreover, the cell line is amenable to biochemical and transcriptional analyses, as we report for GpVI, a direct target of SCL. (ox.ac.uk)
  • Megakaryopoiesis
  • Because of the scarcity of megakaryocytes in hematopoietic tissues, studying megakaryopoiesis heavily relies on the availability of appropriate cellular models. (ox.ac.uk)
  • Thus, the MKD1 cell line offers a pertinent experimental model to study the cellular and molecular mechanisms underlying MkP biology and more broadly megakaryopoiesis. (ox.ac.uk)
  • produces
  • The Aurum total RNA mini kit produces high-quality DNA-free total RNA from a wide range of starting materials including cultured cells, bacteria, and yeast, as well as animal and plant tissues. (bio-rad.com)
  • molecular
  • A key goal in translational cancer research is to understand the molecular abnormalities that underlie the malignant behavior of a cancer cell, and to target them specifically. (aacrjournals.org)