• Macrophages later arrive at the tissue to remove cellular debris, and satellite cells (SCs) are activated and proliferate to induce myofiber regeneration. (biomedcentral.com)
  • In addition to this, we are currently studying cross-talk between the muscle and its extracellular matrix, throughout the muscle as well as specifically at the myotendinous junction, in human models of regeneration and overload. (regionh.dk)
  • Cellular and molecular regulation of muscle regeneration. (scielo.br)
  • Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
  • Directional cell migration is a critical process underlying morphogenesis and post-natal tissue regeneration. (crick.ac.uk)
  • During embryonic myogenesis, migration of skeletal myogenic progenitors is essential to generate the anlagen of limbs, diaphragm and tongue, whereas in post-natal skeletal muscles, migration of muscle satellite (stem) cells towards regions of injury is necessary for repair and regeneration of muscle fibres. (crick.ac.uk)
  • Here, we review mechanisms of myogenic stem/progenitor cell migration during skeletal muscle development and post-natal regeneration. (crick.ac.uk)
  • Skeletal muscle regeneration is regulated by coordinated activation of multiple signaling pathways. (elifesciences.org)
  • However, the role of individual arms of the UPR in skeletal muscle regeneration remain less understood. (elifesciences.org)
  • Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
  • Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
  • Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
  • The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
  • However, the mechanisms of muscle regeneration are not yet fully understood. (elifesciences.org)
  • In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. (nature.com)
  • 20% of the original mass invariably results in functional impairment with limited regeneration capacity, which requires reconstructive surgical procedures such as autologous muscle flap transfers 1 . (nature.com)
  • In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
  • Our current running hypothesis is that the positive growth factors produced by hESCs trigger injured muscle to initiate and maintain regeneration, the TGF-beta inhibitors produced by hESCs reduce the TGF-beta signaling, and the combination assures the robust regeneration of muscle. (ca.gov)
  • Preliminary data indicates the effects on regeneration of old muscle look very promising. (ca.gov)
  • Specifically, our data suggest that several other ligands of MAPK pathway secreted by hESCs are likely to enhance and rejuvenate the regeneration of old muscle tissue. (ca.gov)
  • We have submitted the several manuscripts on topics of enhanced tissue regeneration and we are preparing the manuscript that identifies hESC-based novel strategies for restoring high regenerative capacity to old muscle. (ca.gov)
  • Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
  • Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
  • We investigated the hypothesis that specific energy levels of LIPUS mediates skeletal muscle regeneration by modulating the inflammatory microenvironment. (ijbs.com)
  • LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)
  • In particular, her work has been focusing on the molecular mechanisms involved in the modulation of the peripheral synaptic transmission, skeletal muscle adaptation and regeneration in physiological and pathological conditions. (units.it)
  • The research activity of the group is focused on the identification of new strategies to improve skeletal muscle regeneration. (units.it)
  • Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
  • More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
  • 1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
  • 6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
  • Satellite cells and skeletal muscle regeneration, Compr. (copernicus.org)
  • One of the great advantages of tissue engineering of skeletal muscle is that adult skeletal muscle contains many myogenic precursor stem cells, termed "satellite cells", which are involved in the repair and regeneration of skeletal muscle throughout the lifespan. (josam.org)
  • 3 A certain amount of oxidative stress is important for cellular regeneration allowing for rebuilding of mitochondria in particular as well as cells. (cytoplan.co.uk)
  • Research in the Tidball lab is directed toward understanding processes that regulate skeletal muscle wasting and regeneration. (ucla.edu)
  • Exploring the mechanisms through which the immune system can modulate skeletal muscle wasting, injury, regeneration and growth is a particular focus of the lab. (ucla.edu)
  • Other NIH-funded investigations in our lab explore epigenetic mechanisms through which an anti-aging protein called Klotho affects myogenesis and muscle regeneration in neonatal and aging muscle. (ucla.edu)
  • Defective muscle regeneration can severely affect re-innervation by motor axons, and neuromuscular junctions (NMJs) development, ultimately leading to skeletal muscle atrophy. (biomed.news)
  • In particular, in skeletal muscle, specific semaphorin molecules are involved in the restoration and remodeling of the nerve-muscle connections, thus emphasizing their plausible role to ensure the success of muscle regeneration. (biomed.news)
  • This review article aims to discuss the impact of aging on skeletal muscle regeneration and NMJs remodeling and will highlight the most recent insights about the role of semaphorins in this context. (biomed.news)
  • Muscle cells (including myocytes and muscle fibers) develop from embryonic precursor cells called myoblasts. (wikipedia.org)
  • Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)
  • The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)
  • Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
  • In this project, the group intends to investigate the nature and the role of adenosine and AR signalling pathways on the two isoforms of muscle AChRs: the embryonic (g-AChR) regulating synaptogenesis and muscle development and the adult (e-AChR) with a role in nerve-muscle communication. (units.it)
  • In Drosophila , a population of muscle-committed stem-like cells called adult muscle precursors (AMPs) keeps an undifferentiated and quiescent state during embryonic life. (biologists.com)
  • The embryonic AMPs are at the origin of all adult fly muscles and, as we demonstrate here, they express repressors of myogenic differentiation and targets of the Notch pathway known to be involved in muscle cell stemness. (biologists.com)
  • Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
  • Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
  • On the other hand, despite the atrophy and reduced myogenic potential of the EDL muscles in old rats, when an EDL muscle is cross-age transplanted from an old donor rat into a young host rat, the muscle regenerates as much mass and has as high a capacity for force development as a young muscle transplanted into a young host [4] . (josam.org)
  • Myoblasts fuse from multinucleated skeletal muscle cells known as syncytia in a process known as myogenesis. (wikipedia.org)
  • This occurs during myogenesis with the fusion of myoblasts each contributing a nucleus to the newly formed muscle cell or myotube. (wikipedia.org)
  • embryologically, this multinucleate condition results from multiple myoblasts fusing to produce each muscle fiber, where each myoblast contributes one nucleus. (wikipedia.org)
  • Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. (zen-bio.com)
  • ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes. (zen-bio.com)
  • Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. (zen-bio.com)
  • Skeletal myoblasts are available from both male and female donors. (zen-bio.com)
  • Specially formulated media are available for maintenance and differentiation of our adult skeletal myoblasts. (zen-bio.com)
  • Skeletal muscle progenitors (satellite cells), murine cell line and immortalized human myoblasts are studied in vitro to identify, in a controlled environment, the pathways regulating the regenerative potential of the skeletal muscle. (units.it)
  • Because of the decreased proliferation potential [9] and lower absolute number of satellite cells in muscles from the aged rats compared with adult rats [1,10,11] , one may hypothesize that the aged rats would not yield enough myoblasts to produce myooids in culture. (josam.org)
  • These findings imply a novel and unexpected role for p38 MAPK inhibition in myogenesis and highlight the importance of the limb bud microenvironment in promoting the progression of limb myoblasts. (biologists.com)
  • The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
  • All cells have been tested for myotube differentiation. (zen-bio.com)
  • Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. (zen-bio.com)
  • Analysis both in vivo and in primary satellite-cell cultures, shows that disruption of N-WASp function does not interfere with the program of skeletal myogenic differentiation, and does not affect myoblast motility, morphogenesis and attachment capacity. (huji.ac.il)
  • miR-101a promotes the differentiation of goat skeletal muscle satellite cells (SMSCs), as we previously reported, but the underpinning mechanism remains to be illuminated. (chinagene.cn)
  • These data demonstrated that miR-101a promotes SMSC differentiation directly through EZH2 , which provides a theoretical reference for further elucidating the mechanism of miR-101a in SMSC differentiation. (chinagene.cn)
  • miR-101a targeting EZH2 promotes the differentiation of goat skeletal muscle satellite cells[J]. Hereditas(Beijing), 2017, 39(9): 828-836. (chinagene.cn)
  • MicroRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7. (chinagene.cn)
  • microRNAs in skeletal muscle differentiation and disease. (chinagene.cn)
  • The Wnt signaling pathway is involved in the control of cell proliferation and differentiation during skeletal muscle development. (biomedcentral.com)
  • Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus, Bmc Genomics, 20, 156, https://doi.org/10.1186/s12864-019-5530-7, 2019. (copernicus.org)
  • Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)
  • Myostatin (growth-differentiation factor 8, GDF8) is a member of the transforming growth factor beta (TGFβ) superfamily and a negative regulator of muscle mass. (silverchair.com)
  • We previously showed that bioprinted human skeletal muscle constructs were able to form multi-layered bundles with aligned myofibers. (nature.com)
  • We also demonstrated the feasibility of using these 3D bioprinted human skeletal muscle constructs to treat critical-sized muscle defect injuries with structural and functional restoration in a rodent model 2 . (nature.com)
  • Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
  • Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)
  • Sarcopenia is a progressive, accelerated loss of muscle, accompanied by low physical activity.1 Without a nutritional or exercise intervention muscle loss is inevitable following prolonged bed rest, whether it be due to age, extended illness from winter colds and flus, obesity or from chronic conditions. (healthmasters.com.au)
  • With ageing, skeletal muscle undergoes a severe reduction in tissue mass, leading to a decrease in strength (sarcopenia). (units.it)
  • However, during aging, extensive injury, or pathological conditions, the complete regenerative program is severely affected, with a progressive loss of muscle mass and function, a condition known as sarcopenia. (biomed.news)
  • Satellite cells are considered adult skeletal muscle stem cells that are responsible for the majority of post-birth skeletal muscle growth (maturation and hypertrophy) and adult skeletal muscle homeostasis. (missouri.edu)
  • Models of injury and overload are employed to study the activity of muscle stem cells (satellite cells) and muscle extracellular matrix. (regionh.dk)
  • When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
  • Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)
  • Cell Stem Cell 2013;13(5):590-601. (scielo.br)
  • Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
  • Crist CG, Montarras D, Pallafacchina G, Rocancourt D, Cumano A, Conway SJ, Buckingham M. Muscle stem cell behavior is modified by microRNA-27 regulation of Pax3 expression. (chinagene.cn)
  • Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
  • Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
  • The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)
  • The use of mesenchymal stem cells (MSCs) as a regenerative therapy holds the promise of regrowing injured vasculature, helping DM patients live healthier and longer lives. (thno.org)
  • Meat science and muscle biology symposium: stem cell niche and postnatal muscle growth, J. Anim. (copernicus.org)
  • Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)
  • Therefore, these results establish the efficacy of diprotin A in stimulating muscle cell engraftment, and highlight the pre-clinical utility of a xenotransplantation model in assessing the relative efficacy of muscle stem cell populations. (biomedcentral.com)
  • We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
  • The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)
  • Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)
  • For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)
  • Neurogenesis-on-Chip: Electric field modulated transdifferentiation of human mesenchymal stem cell and mouse muscle precursor cell coculture [med. (emf-portal.org)
  • KIT is a receptor tyrosine kinase type III, which binds to stem cell factor. (inhibitorkit.com)
  • The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development. (biomedcentral.com)
  • The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)
  • Skeletal muscle cells and cardiac muscle cells both contain myofibrils and sarcomeres and form a striated muscle tissue. (wikipedia.org)
  • Skeletal muscle regenerative capacity later diminishes with advancing age and decreasing numbers of SCs, and muscle tissue is steadily replaced by adipose and connective tissues[ 13 ]. (biomedcentral.com)
  • The use of a muscle creatine kinase germline knockout of the gene encoding Ire1/Ern1 shifts the focus from traditional studies of tissue repair toward the regenerating myofibers. (elifesciences.org)
  • More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. (nature.com)
  • These approaches have achieved bioengineered skeletal muscle tissue fabrication with cellular alignment in vitro, and some showed a degree of therapeutic potential in vivo. (nature.com)
  • In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
  • Thirdly, HMB increases the proliferation of satellite cells by the stimulation of Myogenic regulatory factors (MRFs).4 MRFs regulate myogenesis, the formation of muscular tissue. (healthmasters.com.au)
  • She has been reviewer for international Journals ( FASEB J , Cell & Tissue Res , Toxicon, Exp Cell Res ), for national grant agencies (MIUR) and international Foundations (AFM, Association Francaise contre les Myopathies). (units.it)
  • Skeletal muscle is a complex tissue composed of thousands of individual multinucleated myofibers. (arvojournals.org)
  • Prior to tissue engineering of functional skeletal muscle from satellite cells harvested from aged mammals, two key questions must be addressed. (josam.org)
  • To study the capacity of satellite cells to form into functional skeletal muscle tissue de novo in culture, we have developed several in vitro tissue models. (josam.org)
  • The purpose of the current study was to compare the excitability and contractility of myooids engineered from skeletal muscles of 32-month-old rats with that of myooids engineered from adult rat tissue. (josam.org)
  • This process allows the body, particularly muscle tissue, to improve performance over time. (cytoplan.co.uk)
  • To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
  • Neural input into this bioprinted skeletal muscle construct shows the improvement of myofiber formation, long-term survival, and neuromuscular junction formation in vitro. (nature.com)
  • Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)
  • In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
  • 10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)
  • Satellite cells are in general easy to isolate from explanted skeletal muscle, and presumably muscle biopsies from an adult mammal would provide an ample supply of cells committed to the myogenic developmental pathway to facilitate the in vitro engineering of functional skeletal muscle. (josam.org)
  • Myooids were activated by transverse electrical fields in a manner similar to that used to activate whole skeletal muscles in vitro during standard physiological measures of muscle function [8] . (josam.org)
  • Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
  • Skeletal muscle myosin heavy chain (MHC) content was measured and MHC isoforms were separated on SDS-PAGE gels and quantified. (josam.org)
  • Adult and aged rat myooids had only ~35-60% of the skeletal muscle MHC content of control skeletal muscle from rats, the remaining MHC content consisting of isoforms found in cultured fibroblasts but not in control skeletal muscle. (josam.org)
  • In addition, myooids expressed only developmental isoforms of skeletal muscle MHC, known to generate less specific force than adult isoforms. (josam.org)
  • Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)
  • We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)
  • Skeletal muscle constitutes 40% of the body's mass and is designed to generate force for locomotor activity, postural maintenance, and plays an important role in the regulation of systemic energy balance. (missouri.edu)
  • Filipowicz W, Bhattacharyya SN, Sonenberg N. Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight. (chinagene.cn)
  • Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies [med. (emf-portal.org)
  • Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. (chinagene.cn)
  • Only early myogenesis, as assessed by higher MyoD and Myf5 mRNA levels, appeared to be enhanced by hypoxia compared to normoxia. (artinis.com)
  • All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)
  • In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)
  • There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)
  • Normalizing for skeletal muscle MHC content and isoform expression predicts myooid forces of ~6-18% of control adult skeletal muscles. (josam.org)
  • Therefore, our findings increase our knowledge of sulfatase expression diversity and provide a solid basis for further research concerning this molecular mechanism. (scielo.br)
  • Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
  • Nature Reviews Molecular Cell Biology 2012;13(2):127-33. (scielo.br)
  • These changes take place on the molecular level impacting properties of specific the different parts of cell inner structure, but resulting in alterations in mechanical properties of the complete cell ultimately. (globaltechbiz.com)
  • To understand the function of SFRP1 and miRNA-1/206 in swine myogenesis, we first predicted the targets of miRNA-1/206 with the TargetScan and PicTar programs, and analyzed the molecular characterization of the porcine SFRP1 gene. (biomedcentral.com)
  • Additionally, our data in progress suggest that muscle and brain age by similar molecular mechanisms and thus, therapeutic strategies for rejuvenating muscle repair might be applicable to the restoration of neurogenesis in aged brain. (ca.gov)
  • Molecular cell. (illinois.edu)
  • Here, we used chickens, the most common agricultural animals worldwide, to determine the genetic and molecular mechanisms of negative heterosis. (biomedcentral.com)
  • In living organisms, if the temperature exceeds the normal range (thermo-neutral zone), it disturbs the normal physiological functioning and induces cell injury. (frontiersin.org)
  • These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)
  • Skeletal muscle is characterized by a remarkable capacity to rearrange after physiological changes and efficiently regenerate. (biomed.news)
  • Originally discovered as axon guidance factors, they have been implicated in cancer progression, embryonal organogenesis, skeletal muscle innervation, and other physiological and developmental functions in different tissues. (biomed.news)
  • in these cells physiological lev els of E2 and low levels of xenoestrogens can rapidly reverse actions of the DAT. (inhibitorkit.com)
  • In this study we exam ined both indirect and direct mechanisms involved in physiological estrogen mediated dopamine efflux in Inhibitors,Modulators,Libraries con junction with the cellular location of the ERs and the DAT. (inhibitorkit.com)
  • Expression of both M. amblycephala Hb subunits was down-regulated in most examined tissues and time points post-Aeromonas hydrophila infection, which might be due to red blood cell (RBC) and hematopoietic organ damage. (bvsalud.org)
  • Also, a vascularization strategy using co-culture with endothelial cells (ECs) and fibroblasts improved the survival of the bioengineered skeletal muscle tissues 20 , 21 . (nature.com)
  • What was surprising is that administering these inhibitors to the whole animal appears to reduce TGF-beta levels in the whole animal, suggesting some kind of feed-back and perhaps effects on other tissues as well as muscle. (ca.gov)
  • Both a temporal correlation between CVB infection that precedes seroconversion of autoantibodies to β-cell antigens, and a spatial association of CVB with insulitis in pancreatic tissues from T1D patients have been observed. (institutcochin.fr)
  • The latter expressed the PD-1 marker, suggesting an exhausted phenotype, and shared expanded, yet private, antigen T-cell receptors (TCRs) with peripheral tissues. (institutcochin.fr)
  • Genome-wide gene expression pattern analyses of breast muscle tissues revealed that nonadditivity, including dominance and overdominace, was the major gene inheritance pattern. (biomedcentral.com)
  • Investigating the nature of active forces in tissues reveals how contractile cells can form extensile monolayers. (u-paris.fr)
  • Acute hypoxia has previously been suggested to potentiate resistance training-induced hypertrophy by activating satellite cell-dependent myogenesis rather than an improvement in protein balance in human. (artinis.com)
  • Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)
  • This property is primarily attributable to satellite cells which comprise a small population of quiescent mono-nucleated cells which first appear during late fetal life, and which reside between the basal lamina and the surface (sarcolemma) of mature skeletal muscle cells. (missouri.edu)
  • and the cell membrane in a muscle cell is termed the sarcolemma. (wikipedia.org)
  • In skeletal muscle, at the end of each muscle fiber, the outer layer of the sarcolemma combines with tendon fibers at the myotendinous junction. (wikipedia.org)
  • Notably, a given muscles fiber-type composition is not static, but instead, fiber-type composition is remarkably adaptable (fastàslow-twitch fibers or slowàfast-twitch fibers) to a broad spectrum of stimuli that include altered weight-bearing (increase load bearing, zero gravity), endocrine factors, altitude, endurance & resistance training, and diet (high fat vs high carbohydrate). (missouri.edu)
  • Previous work has shown that slow-oxidative fibers are associated with more satellite cells per-unit length than are fast-glycolytic myofibers. (missouri.edu)
  • Our current studies are designed to identify potential signaling pathways connecting increased TEAD-1 expression in skeletal muscle to activation of genes encoding proteins typically restricted to slow-twitch fibers, as well as satellite cell numbers that exceed homeostatic numbers associated with slow-fibers. (missouri.edu)
  • Skeletal muscle cells are the individual contractile cells within a muscle and are more usually known as muscle fibers because of their longer threadlike appearance. (wikipedia.org)
  • A single muscle such as the biceps brachii in a young adult human male contains around 253,000 muscle fibers. (wikipedia.org)
  • Skeletal muscle fibers are the only muscle cells that are multinucleated with the nuclei usually referred to as myonuclei. (wikipedia.org)
  • The cell membrane is anchored to the cell's cytoskeleton by anchor fibers that are approximately 10 nm wide. (wikipedia.org)
  • A fundamental aspect of skeletal myogenesis involves extensive rounds of cell fusion, in which individualmyoblasts are incorporated into growing muscle fibers. (huji.ac.il)
  • WASp-family elements appear therefore to act as universal mediators of the myogenic cell-cell fusion mechanism underlying formation of functional muscle fibers, in both vertebrate and invertebrate species. (huji.ac.il)
  • 3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)
  • Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
  • Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)
  • These findings reveal a specific role for N-WASp during mammalian myogenesis. (huji.ac.il)
  • Mammalian hibernation presents a unique scenario, where animals are hypocaloric and in torpor, but the extent of muscle protein loss is minimized. (silverchair.com)
  • Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
  • The addition of BrdU-positive myonuclei increased from 1 per 1000 myofibers in cross sections of control muscles to 2 to 3 per 100 myofibers in the resected muscles. (arvojournals.org)
  • It is considered postmitotic, in that the myonuclei residing within these myofibers do not divide in adult muscle. (arvojournals.org)
  • Nonetheless, the enhanced myogenesis leads to the formation of an extensive network of contractile myofibers, and this enhanced myogenesis can be conferred upon myogenic cells from clonal populations, such as G8 or C2C12 cells, if they are co-cultured with the limb mesenchymal cells. (biologists.com)
  • Immortalized Canine Dystrophic Myoblast Cell Lines for Development of Peptide-Conjugated Splice-Switching Oligonucleotides. (institut-myologie.org)
  • Enhancing Myoblast Fusion and Myotube Diameter in Human 3D Skeletal Muscle Constructs by Electromagnetic Stimulation [med. (emf-portal.org)
  • The past two decades has seen major advances towards our current understanding of skeletal myogenesis (reviewed in Perry and Rudnicki, 2000 ). (biologists.com)
  • Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
  • Panobinostat therapy significantly repressed mRNA for DNMT1 and DNMT3a in the two cell lines while no modifications had been observed in DNMT3b levels. (thrombininhibitors.com)
  • Depletion of macrophages in vivo eliminated the therapeutic effects of LIPUS, indicating that improvement in muscle function was the result of M2-shifted macrophage polarization. (ijbs.com)
  • Alternatively activated M2 macrophages comprise cells exposed to low concentrations of M-CSF in the presence of IL-4, IL-13, or IL-10. (hindawi.com)
  • For example, our findings have shown that macrophages and eosinophils are key effector cells in the pathogenesis of Duchenne muscular dystrophy. (ucla.edu)
  • However, discoveries in our lab have also shown that regulatory interactions between cytotoxic, M1 macrophages in dystrophic muscle and anti-inflammatory, M2 macrophages are important in regulating the balance between the death of dystrophic muscle and regenerative processes. (ucla.edu)
  • Duchenne muscular dystrophy (DMD) is the most common muscular dystrophy and an X-linked recessive, progressive muscle wasting disease caused by the absence of a functional dystrophin protein. (biomedcentral.com)
  • Although administration of various myogenic cell types has been done intramuscularly or intravascularly, functional restoration has not been achieved yet in patients with degenerative diseases affecting multiple large muscle. (crick.ac.uk)
  • A bioengineered skeletal muscle construct that mimics structural and functional characteristics of native skeletal muscle is a promising therapeutic option to treat extensive muscle defect injuries. (nature.com)
  • We reported that LIPUS therapy at intensity of 60mW/cm 2 exhibited the most significant differences in functional recovery of contusion-injured muscle in mice. (ijbs.com)
  • The comprehensive functional tests and histological analysis in vivo indirectly and directly proved the effectiveness of LIPUS for muscle recovery. (ijbs.com)
  • To facilitate functional analysis of anti-CVB CD8+ T-cell responses, CD8+ T-cell transductants were generated by expressing TCRs recognizing immunodominant CVB peptides in primary CD8+ T cells and then used in real-time imaging cytotoxic assays. (institutcochin.fr)
  • Several types of adenosine receptors have been detected in developing and differentiated skeletal muscle cells, but little is known about the functional outcome of AR modulation on muscle AChRs. (units.it)
  • Functional three-dimensional skeletal muscle constructs (myooids) were engineered from myogenic cells harvested from the muscles of adult and aged rats. (josam.org)
  • First, to what extent are the satellite cells from aged mammals capable of fusion into functional myotubes, and second, do satellite cells from aged mammals have any form of impaired function when compared with those from young or adult mammals? (josam.org)
  • These changes include elongation, polarization, aggregation and fusion, which ultimately give rise to functional muscle. (biologists.com)
  • The formation of skeletal muscle:from somite to limb. (scielo.br)
  • 7 8 9 In fact, passive stretching alone can activate limb muscle satellite cells. (arvojournals.org)
  • Here we show that, concurrent with a decrease in chondrogenesis, inhibition of p38 in limb bud cultures dramatically promotes muscle development. (biologists.com)
  • This activity, developed with the full support of the AFM as well as the Dutch Duchenne Parent Project NL (DPP-NL) and the Jain Foundation, has resulted in the creation of a platform for the immortalisation of human cells. (institut-myologie.org)
  • Therefore, we sought to translate these results to a clinically relevant canine-to-canine allogeneic transplant model for Duchenne muscular dystrophy (DMD) and determine if CXCR4 is important for donor cell engraftment. (biomedcentral.com)
  • One of the key reasons for this negative outcome is the limited migration of donor cells, which hinders the overall cell engraftment potential. (crick.ac.uk)
  • Together, these data suggest that CXCR4 plays an important role in donor cell engraftment. (biomedcentral.com)
  • We show that CXCR4 expressing cells are important for donor muscle cell engraftment, yet FACS sorted CXCR4-positive cells display decreased engraftment efficiency. (biomedcentral.com)
  • However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
  • Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
  • We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)
  • Introduction Cell mechanics has proved to be a widely used label-free biomarker to discern phenotypes, detect pathologies and more importantly, monitor existence or progression of a disease [1C3]. (globaltechbiz.com)
  • Secondly, they can improve our understanding of the adjustments that occur during disease progression which, in turn, might uncover reasons for corresponding alterations occurring in cell mechanics [10, 11]. (globaltechbiz.com)
  • The intent of vaccination is to induce a combined antibody and T-cell anti-HER-2 immune response, which is intended to target HER-2-expressing tumor cells, and may induce tumor regression or slow progression of disease. (stanford.edu)
  • 2002). Divergence in species and regulatory role of beta -myosin heavy chain proximal promoter muscle-CAT elements. (missouri.edu)
  • 2001). Multiprotein complex formation at the beta myosin heavy chain distal muscle CAT element correlates with slow muscle expression but not mechanical overload responsiveness. (missouri.edu)
  • The thin myofilaments are filaments of mostly actin and the thick filaments are of mostly myosin and they slide over each other to shorten the fiber length in a muscle contraction. (wikipedia.org)
  • In striations of muscle bands, myosin forms the dark filaments that make up the A band. (wikipedia.org)
  • No effects were found on myosin heavy chain expression, markers of oxidative metabolism and lactate transport in the skeletal muscle. (artinis.com)
  • A striated muscle fiber contains myofibrils consisting of long protein chains of myofilaments. (wikipedia.org)
  • Dystrophin has a structural role as a cytoskeletal stabilization protein and protects cells against contraction-induced damage. (biomedcentral.com)
  • The unfolded protein response (UPR) is a major mechanism that detects and alleviates protein-folding stresses in the endoplasmic reticulum. (elifesciences.org)
  • Muscle fractional protein synthetic rate was measured over the whole period by deuterium incorporation into the protein pool and muscle thickness by ultrasound. (artinis.com)
  • At the end of the training protocol, the strength gain was higher in the hypoxic vs the normoxic group despite no changes in muscle thickness and in the fractional protein synthetic rate. (artinis.com)
  • The UPS is a crucial protein degradation system in human cells. (healthmasters.com.au)
  • Interestingly, a CVB epitope homologous to an epitope of the β-cell protein Glutamic Acid Decarboxylase (GAD), a major autoantigen of T1D, was identified and recognized by the same CD8+ T cells, demonstrating cross-reactivity. (institutcochin.fr)
  • however, priority needs to be given to resistance exercise training, and secondarily to adequate protein intake should be part of this dietary regime to mitigate losing muscle mass. (biomed.news)
  • These results suggest that the 3D bioprinted human neural-skeletal muscle constructs can be rapidly integrated with the host neural network, resulting in accelerated muscle function restoration. (nature.com)
  • These constructs (mm 3 -cm 3 scale) consisted of tens to hundreds of long parallel myofiber bundles, containing densely packed, highly viable, and aligned muscle cells. (nature.com)
  • While host nerve integration and formation of NMJs were evident within the implanted skeletal muscle constructs, we observed that the constructs did not support the full restoration of defected muscles at 8-week post-implantation, which is likely due to the delayed integration of host nerve. (nature.com)
  • These cells are located beneath the basal lamina of myofiber in a dormant state. (elifesciences.org)
  • The main interest of her research activity is the nerve-muscle communication and the skeletal muscle physiology, including muscle plasticity and impact of ageing on the neuromuscular system. (units.it)
  • The results of the proposed research will advance the understanding of important aspects of the neuromuscular physiology such as the modulation of the neuromuscular transmission and the skeletal muscle plasticity. (units.it)
  • In addition, cytokines released in the process of myonecrosis recruit inflammatory cells, which release inflammatory cytokines to activate fibroblasts that lay down extracellular matrix proteins and lead to fibrosis[ 12 ]. (biomedcentral.com)
  • In more detail, the team is studying the role of electrical activity and local trophic factors in the microenvironment of satellite cell niche and in the post-mitotic myogenesis. (units.it)
  • Fusion depends on muscle-specific proteins known as fusogens called myomaker and myomerger. (wikipedia.org)
  • The cell membrane is really a viscous fluid-like matter which includes several lipids, cholesterol, and inserted proteins. (globaltechbiz.com)
  • In the next reporting period we will re-confirm that the levels of candidate proteins from the 500 antibody array actually are very highly produced by hESCs and that the signals from these proteins are perceived by regenerating muscle cells. (ca.gov)
  • In 2010 beginning of 2011, we have approached the identification and characterization of the proteins that are produced by hESCs and have the rejuvenating and pro-regenerative activity on adult muscle. (ca.gov)
  • Mitochondrial biogenesis (synthesis of new mitochondria) is constantly ongoing within skeletal muscle in order to maintain mitochondrial content and function in response to various stimuli including exercise, as well as other cellular stressors and oxidative stress post-training stimulates increased mitochondria production. (cytoplan.co.uk)
  • Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)
  • Enforced expression of miR-101 inhibits prostate cancer cell growth by modulating the COX-2 pathway in vivo . (chinagene.cn)
  • Myofiber-specific ablation of IRE1α dampens Notch signaling and canonical NF-κB pathway in skeletal muscle of adult mice. (elifesciences.org)
  • In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
  • In this light, they will be useful for identification of new pharmacological tools to control the activity of muscle AChRs and, thus, for finding novel strategies for neuromuscular diseases associated with altered neuromuscular transmission. (units.it)
  • The IAL aims to synergise the expertise in muscle and neuromuscular diseases of French teams and the expertise in immunology and inflammation of the Brazilian teams . (institut-myologie.org)
  • Myogenesis is a multi-step process through which uncommitted mesodermal cells commit, differentiate and undergo profound phenotypic changes. (biologists.com)