EmbryonicCardiomyocyte differentiationPluripotentMyocardialProgenitor cellsProliferation and differentiationMolecularLineageLeft ventricularPathologicalProgenitorsIschemiaVitroCardiomyocytes from humanMesenchymalRegenerationInhibitionDefectsGene expressionSignaling pathwayStem cellMaturationProteinsDysfunctionInducesPrecursor cellsEmbryogenesisIPSCsEpigeneticGenesPromotesRegulatesFibrosisTissueLineagesRegulationProteinRegenerativeCardiovascularMitochondrial functionFetalInflammationBiologyTherapyFibroblastHESCsCoronaryFunctionDifferentiateHuman umbilical vein endothelial cellsFunctional
Embryonic21
- Remarkably, the progenitors derived from embryonic stem cells by dual inhibition efficiently differentiate into cardiac and skeletal muscle cells. (ncbs.res.in)
- Notch signaling is highly conserved cell signaling pathway, which is involved in diverse embryonic organs or tissue development as well as regeneration ( 1 - 10 ). (frontiersin.org)
- Generating specific cell lineages from induced pluripotent stem cells and embryonic stem cells is the holy grail of regenerative medicine. (shinshu-u.ac.jp)
- The team, led by Associate Professor Tomohide Takaya from the Department of Agricultural and Life Sciences at Shinshu University, recently discovered that an anti-nucleolin aptamer, myogenetic oligodeoxynucleotide iSN04, induced myocardial differentiation in embryonic stem cells (ESCs). (shinshu-u.ac.jp)
- Furthermore, genetic ablation or reduction of DNA methylation in embryonic stem cells or cardiac myocytes, respectively, does not alter genome-wide chromatin organization. (galaxyproject.org)
- We developed an improved protocol for isolating embryonic myocyte cells, for use in co-cultures with epicardial cells. (arizona.edu)
- Promotes self-renewal and maintains pluripotency of human embryonic stem cells and mouse Epi-stem cells when used in combination with CHIR99021 (Kim et al. (stemcell.com)
- Here we examined protein kinase signaling and mechanics in response to dynamic vs. static stiffness during the commitment process from embryonic stem cells (ESCs) through cardiomyocytes to better understand how developmentally-appropriate temporal changes in stiffness regulate cell commitment. (silverchair.com)
- As P19 cells have differentiation ability, they can be useful in investigating complex biological processes such as tissue formation and early embryonic development. (cytion.com)
- Modeling mitosis of embryonic stem cells, magnified in the microscope. (cytion.com)
- P19 embryonic carcinoma cell line is adherent. (cytion.com)
- A humidified incubator set at 37°C with a 5% CO2 supply is essential to growing and culturing the P19 embryonic carcinoma cell line. (cytion.com)
- Pluripotent embryonic carcinoma cell line P19 is a useful model for studying early embryonic development. (cytion.com)
- More recently, we have initiated studies to investigate the molecular mechanisms that control the development of distinct myogenic cell lineages during embryonic mygenesis. (rosalindfranklin.edu)
- These lineages are defined are distinct types of embryonic myoblasts that are committed to the differentiation of diverse, lineage-based, muscle fiber types. (rosalindfranklin.edu)
- Additionally, PSCs - both embryonic stem cells and induced pluripotent stem cells - provide insights into the mechanisms involved in the differentiation and specification of heart cells. (ucla.edu)
- By performing promoter capture Hi-C (PCHi-C) on human embryonic stem cell-derived cardiomyocytes (hESC-CMs), we show that such promoter interactions are a key mechanism by which enhancers contact their target genes after hESC-CM differentiation from hESCs. (manchester.ac.uk)
- By employing microRNA expression profiling and functional knockdown studies on human embryonic stem cell-derived cardiomyocytes, the authors identified miR-363 as an upstream negative regulator of left ventricular specification transcription factor HAND1 . (biomedcentral.com)
- miRNAs could thus be the best targets for understanding cardiac specialization during differentiation of human embryonic stem cells (hESCs). (biomedcentral.com)
- It is reactivated by cardiac stresses and forms a complex with its embryonic partners, HDAC and PARP, to induce a pathological alpha-MHC to beta-MHC shift. (ca.gov)
- Our studies show that Brg1 maintains cardiomyocytes in an embryonic state, and demonstrate an epigenetic mechanism by which three classes of chromatin-modifying factors-Brg1, HDAC and PARP-cooperate to control developmental and pathological gene expression. (ca.gov)
Cardiomyocyte differentiation2
- loss of function and missense variants during cardiomyocyte differentiation of isogenic human induced pluripotent stem cells. (ox.ac.uk)
- Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. (rndsystems.com)
Pluripotent17
- Transcription factor GATA4-targeted compounds that have previously shown in vivo efficacy in cardiac injury models were tested for stage-specific activation of atrial and ventricular reporter genes in differentiating pluripotent stem cells using a dual reporter assay. (biomedcentral.com)
- Significantly, this is the first report of directed differentiation of pluripotent stem cells, without transgenes, into progenitors with muscle/heart dual potential. (ncbs.res.in)
- By demonstrating the generation of cardiomyocytes from murine pluripotent stem cells, their concept shows promise as a regenerative therapy. (shinshu-u.ac.jp)
- An anti-nucleolin DNA aptamer, iSN04, regulates cardiomyogenesis of murine pluripotent stem cells. (shinshu-u.ac.jp)
- Self-renewal and pluripotency-the capacity to form any cell lineage-are inherent characteristics of induced pluripotent stem cells (iPSCs). (shinshu-u.ac.jp)
- We had previously found that iSN04 promoted myogenic precursor cells (myoblasts) to differentiate into skeletal muscle cells and had hypothesized that the aptamer also enhanced differentiation of pluripotent stem cells. (shinshu-u.ac.jp)
- However, when these pluripotent stem cells were treated after experiencing differentiating conditions for five days, specific marker genes were upregulated, and the cells committed to forming beating cardiomyocytes. (shinshu-u.ac.jp)
- Promotes differentiation of cardiomyocytes from human pluripotent stem cells (PSCs) that have been induced to mesoderm by addition of Activin A and/or BMP4 (Ren et al. (stemcell.com)
- P19 is a type of pluripotent embryonal carcinoma cells originally obtained from teratocarcinoma developed in a C3H/He mouse. (cytion.com)
- I am interested in modelling genetic heart diseases using patient specific induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs). (sluijterlab.com)
- Dr. Ardehali uses novel transgenic mouse models, as well as mostly human pluripotent stem cells (PSC) to address his research questions. (ucla.edu)
- The efficient derivation of specialized and functional cardiomyocytes (CMs) from pluripotent stem cells is a primary goal for stem cell-based cardiac regenerative therapies. (biomedcentral.com)
- Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells. (rndsystems.com)
- Co-differentiation and Co-maturation of Human Cardio-pulmonary Progenitors and Micro-Tissues from Human Induced Pluripotent Stem Cells. (rndsystems.com)
- Recapitulating human cardio-pulmonary co-development using simultaneous multilineage differentiation of pluripotent stem cells. (rndsystems.com)
- Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. (rndsystems.com)
- In this seminar, I will first present how we used human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes to decipher the molecular pathways that underlie HCM in NS. (armi.org.au)
Myocardial10
- The abnormal morphology and dysfunction of mitochondria have been proven as the principal mechanisms in the pathogenesis of cardiovascular diseases, such as heart failure, myocardial infarction, atherosclerosis, and hypertension ( 4 - 6 ). (frontiersin.org)
- The idea of myocardial regeneration with stem cell transplantation after myocardial infarction receives tremendous interest. (escardio.org)
- However these therapies are of limited value for restoration of cardiac functions after myocardial infarction. (escardio.org)
- Several preliminary reports have demonstrated that local stem cell transplantation in patients with acute myocardial infarction is safe and may lead to improved myocardial function and perfusion. (escardio.org)
- Interestingly, contradictory data exist for transformation of bone marrow derived progenitor cells (BMPC) into new cardiomyocytes and alternative mechanisms as enhanced neovascularization, enhanced scar tissue formation due to augmented inflammatory response and decreased apoptosis have been suggested for the benefical effects of these cells on myocardial function after myocardial infarction (4). (escardio.org)
- Myocardial fibrosis, characterized as interstitial fibroblast proliferation and excessive collagen deposition, is the structural basis of myocardial stiffness and the key process of cardiac function transformation from the compensatory phase to heart failure. (dovepress.com)
- 1 Thus, it is imperative to understand the mechanisms involved in inhibition of myocardial fibrosis. (dovepress.com)
- Recently, EndMT has been increasingly recognized as a vital process that contributes to various cardiovascular pathologies in adults, which include atherosclerosis, valvular heart disease, cardiac fibrosis, and myocardial infarction. (dovepress.com)
- Reperfusion after cardiac ischemia increases cell death and infarct size (IS), called myocardial ischemia/reperfusion (I/R) injury, which is the main cause of myocardial injury during the cardiac surgery particularly in coronary artery bypass graft surgery ( 1 , 2 ). (spandidos-publications.com)
- Hypoxia/reoxygenation (H/R) as well as hypoxic preconditioning (HPC) is widely used for simulating in vivo myocardial I/R and ischemic preconditioning (IPC) in a cell culture model. (spandidos-publications.com)
Progenitor cells7
- Chemical compounds that modulate atrial and ventricular cell fate could be used to improve subtype-specific differentiation of endogenous or exogenously delivered progenitor cells in order to promote cardiac regeneration. (biomedcentral.com)
- Collectively, these results indicate the potential for therapeutic alteration of cell fate decisions and pathological gene regulatory networks by GATA4-targeted compounds modulating chamber-specific transcriptional programs in multipotent cardiac progenitor cells and cardiomyocytes. (biomedcentral.com)
- Thus, small molecules capable of influencing the fate decisions and differentiation programs of multipotent progenitor cells could facilitate therapeutic regeneration of lost myocardium. (biomedcentral.com)
- The bone morrow contains several stem cell types including hematopoetic stem cells, endothelial progenitor cells, mesenchymal (stromal) stem cells and multipotent adult progenitor cells. (escardio.org)
- Although there is no definitive evidence, combinations of progenitor cells seem to be more benefical than specific stem cell type for cardiac repair (3). (escardio.org)
- There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
- Of particular interest to Dr. Ardehali is the intrinsic signaling that trigger cardiac regeneration early in life, molecular events that regulate developmental decisions instructing cardiac progenitor cells to adopt a specific cell fate, and delivery approaches of cardiovascular progenitors into an injured heart. (ucla.edu)
Proliferation and differentiation3
- These studies provide insight into mechanisms that regulate not only muscle cell proliferation and differentiation, but regulation of cell proliferation in general. (rosalindfranklin.edu)
- Identifying molecular regulators and critical mediators of cardiac cell type development, proliferation, and differentiation is of great clinical importance, and unraveling such molecular horizons could lead to the development of therapeutic strategies for successful regeneration of the human adult heart. (biomedcentral.com)
- A leading hypothesis proposes that tubular cell proliferation and differentiation are linked to flow rate and that ciliary dysfunction may thus lead to cystic transformation. (msdmanuals.com)
Molecular19
- The general principles of cardiac development have been studied in great detail on a morphological and molecular basis. (nature.com)
- Using various assays like RNA sequencing, cell staining and imaging, and molecular interaction and pathway analysis, the researchers investigated iSN04's effect on murine ESCs and iPSCs. (shinshu-u.ac.jp)
- and trying to understand it at the molecular level and addressing the most important compounds, especially the natural ones, and their proposed mechanism of action against Dox. (mosuljournals.com)
- Renu K, Abilash VG, PB TP, Arunachalam S. Molecular mechanism of doxorubicin-induced cardiomyopathy-An update. (mosuljournals.com)
- Doxorubicin-induced cardiomyopathy: From molecular mechanisms to therapeutic strategies. (mosuljournals.com)
- Rawat PS, Jaiswal A, Khurana A, Bhatti JS, Navik U. Doxorubicin-induced cardiotoxicity: An update on the molecular mechanism and novel therapeutic strategies for effective management. (mosuljournals.com)
- Researchers use P19 cells to elucidate cell signaling pathways and cellular and molecular mechanisms involved in these processes. (cytion.com)
- Ion channels have long been key therapeutic targets in disease intervention and pharmaceutical drug development because of their direct involvement in diverse diseases, vulnerability to small molecular modulation, and accessibility for direct activity measurement on cell membranes by patch-clamp recording from whole cell to single molecule levels. (mdanderson.org)
- Much of our research focuses on the cellular and molecular mechanisms that regulate the development and differentiation of skeletal muscle fibers and their respective phenotypes. (rosalindfranklin.edu)
- The aim of this research is to identify and characterize the molecular regulatory circuitry that establishes these myogenic cell lineages and which thereby establish fast versus fast/slow muscle fiber types, independent of innervation, and dependent of cell lineage commitment. (rosalindfranklin.edu)
- Postdoctoral fellows have the opportunity to investigate numerous aspects of the cellular and molecular mechanisms of skeletal and cardiac muscle development. (rosalindfranklin.edu)
- We aim to elucidate the molecular and functional mechanisms behind the pathological effect of the genetic heart diseases. (sluijterlab.com)
- Rejuvenation therapies aim to reverse or repair age-related cellular changes such as molecular waste, calcification , tissue stiffening , loss of stem cell function , genetic alterations, and impaired energy production . (fightaging.org)
- Zebrafish Fin: Complex Molecular Interactions and Cellular Mechanisms Guiding Regeneration. (uni-ulm.de)
- This is a fascinating time to be involved in the field of cellular differentiation, with so many advances being made in the understanding of molecular events and the application of this knowledge in the biomedical sphere. (yorku.ca)
- Such a prospect is currently hampered, however, in part by an incompletely defined complex of molecular regulators of cardiac cell development and differentiation. (biomedcentral.com)
- Moreover, the molecular mechanisms underlying cardiogenesis defects in NS remain poorly understood and an important need lingers to uncover therapeutic strategies through the in-depth investigation of the molecular causes of CHDs and cardiomyopathies in NS. (armi.org.au)
- I will discuss how we can leverage our hPSC-based platform to study cardiogenesis and cardiac function in genetic disorders as a unique opportunity to discover the molecular perturbations responsible for CHDs or cardiomyopathies. (armi.org.au)
- Fabrice later joined Beth Israel Deaconess Medical Center, Harvard Medical School where he used hiPSCs to delineate the molecular mechanisms underlying hypertrophic cardiomyopathy in RASopathies. (armi.org.au)
Lineage7
- T helper 17 lineage differentiation is programmed by orphan nuclear receptors RORα and RORγ. (nature.com)
- an anti-nucleolin DNA aptamer, iSN04, can determine a cell's lineage during differentiation. (shinshu-u.ac.jp)
- iSN04 treatment under differentiating conditions inhibited stem cell commitment to the cardiac lineage. (shinshu-u.ac.jp)
- Ultimately, terminal cell differentiation commits to the cardiomyocyte lineage. (shinshu-u.ac.jp)
- Endothelial cell lineage tracing showed that BNP directly stimulated the proliferation of resident endothelial cells via NPR-A binding and p38 MAP kinase activation. (elifesciences.org)
- cell lineage characterized by transient expression that contributes to hemogenic endothelium and endocardium, suggesting a novel role for in hemoangiogenic lineage specification and diversification. (baxkyardgardener.com)
- 2006). Cardiovascular progenitors sequentially express the cardiac transcription factors (and, in response to cues from the microenvironment, undergo lineage diversification and differentiation (Laugwitz et al. (baxkyardgardener.com)
Left ventricular2
- Rats injected with ISO exhibited severe interstitial cardiac fibrosis and perivascular fibrosis, decreased left ventricular ejection fraction, and increased NOX activity. (dovepress.com)
- TMZ treatment mitigated cardiac fibrosis, ameliorated left ventricular dysfunction, and reduced NOX activity. (dovepress.com)
Pathological4
- In pathological conditions, damaged cells transfer dysfunctional mitochondria toward recipient cells to ask for help and take up exogenous functional mitochondria to alleviate injury. (frontiersin.org)
- Regulation of arteriovenous specification and differentiation in both endothelial cells and vascular smooth muscle cells are also involved in Notch signaling including regulation of blood vessel sprouting, branching during normal and pathological angiogenesis, and the physiological responses of vascular smooth muscle cells ( 4 , 6 , 7 , 21 - 23 ). (frontiersin.org)
- MicroRNAs regulate target gene expression post-transcriptionally in a myriad of cell types and play critical roles in diverse physiological and pathological processes, including cardiomyocyte development, differentiation, and regeneration. (biomedcentral.com)
- He obtained his PhD at the Sorbonne University in Paris, where he explored the function of serotonin receptors in pathological cardiac hypertrophy. (armi.org.au)
Progenitors4
- Furthermore, it is unknown whether endogenous progenitors/stromal cells in the adult human heart could be chemically induced to generate functional atrial or ventricular heart muscle to treat adult heart diseases. (biomedcentral.com)
- We uncovered two mechanisms of nucleolin interference with iSN04 at play whereby early treatment inhibits cardiomyogenesis, while treatment at a later stage enhances the generation of cardiac progenitors. (shinshu-u.ac.jp)
- Cardiac progenitors Th surviving in the heart buy 349085-38-7 crescent are shaped through the first cardiovascular field (FHF) situated in the anterior lateral dish mesoderm (LPM). (baxkyardgardener.com)
- 2006). For buy 349085-38-7 example, bipotent SHF progenitors generate endocardium or easy muscle cells as well as cardiomyocytes (Lescroart et al. (baxkyardgardener.com)
Ischemia2
- The resistant state of skeletal myoblasts to ischemia renders these cells suitable candidates for repair of chronically infracted and failing heart (5). (escardio.org)
- In this review, we focus on the role of cardiomyocyte-derived and cardiac fibroblast-derived microRNAs that are involved in the regulation of genes associated with cardiomyocyte and fibroblast function and in atherosclerosis-related cardiac ischemia. (archivesofmedicalscience.com)
Vitro3
- An in vitro model of EndMT was developed using human umbilical vein endothelial cells (HUVECs) via treatment with transforming growth factor-β (TGF-β) at 10 ng/mL for 24 h. (dovepress.com)
- Neural crest stem cells from human epidermis of aged donors maintain their multipotency in vitro and in vivo. (rndsystems.com)
- In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)
Cardiomyocytes from human1
- Induces differentiation of cardiomyocytes from human ESCs and iPSCs. (rndsystems.com)
Mesenchymal5
- This co-culture system allows investigation into the effects of myocyte derived soluble factors uponepicardial EMT and mesenchymal cell differentiation. (arizona.edu)
- The origin of new endothelial cells (i.e. resident or infiltrating) as well as the underlying mechanism leading to their proliferation (partial endothelial-to-mesenchymal transition [EndMT] or not) have long been debated. (elifesciences.org)
- Endothelial-to-mesenchymal transition (EndMT) is an important mechanism underlying cardiac fibrosis. (dovepress.com)
- Cardiac fibroblasts are generally the primary effector cells of fibrosis and have been reported to be partly derived from cardiac endothelial cells through the endothelial to mesenchymal transition (EndMT) process. (dovepress.com)
- 2 EndMT is a process by which endothelial cells lose a portion of their cellular features and obtain the mesenchymal phenotype, including the loss of tight junctions and increased production of extracellular matrix. (dovepress.com)
Regeneration3
- Transgenic mouse models are instrumental for elucidating pathways that regulate cardiac development and regeneration. (ucla.edu)
- Wnt/β-catenin signaling acts cell-autonomously to promote cardiomyocyte regeneration in the zebrafish heart. (uni-ulm.de)
- Recently, miRNAs have been recognized as important players in cardiac development, pathology, and regeneration. (biomedcentral.com)
Inhibition5
- SR1001 inhibited the development of murine T H 17 cells, as demonstrated by inhibition of interleukin-17A gene expression and protein production. (nature.com)
- Therefore, our findings provide compelling evidence that dual inhibition specifies head mesoderm and unravel the mechanism that diversifies head and trunk muscle programmes during early mesoderm fate commitment. (ncbs.res.in)
- In this review, we will summarize the cardiac effects of thioredoxin and the mechanisms by which thioredoxin mediates inhibition of ventricular remodeling. (nih.gov)
- This study aimed to investigate the possible effect of TMZ on cardiac fibrosis exerted via the inhibition of NOX2-mediated EndMT. (dovepress.com)
- Upregulation of miR‑133b‑5p contributes to HPC‑mediated cardioprotection in cardiomyocytes, and the mechanism may be associated with inhibition of caspase‑8 and caspase‑3 apoptotic signaling. (spandidos-publications.com)
Defects4
- Several proteins that interact with MYH6 are also associated with cardiac defects. (wikipedia.org)
- variants cause cardiac outflow tract defects, sometimes with pancreatic and diaphragmic malformations. (ox.ac.uk)
- Another T-box gene, TBX1, is involved in velo-cardio-facial syndrome Diverge syndrome, the most common deletion which has extensive symptoms including defects of the cardiac outflow tract including tetralogy of Fallot. (imedpub.com)
- 1) Antibody deficiency disorders are defects in immunoglobulin-producing B cells . (lu.se)
Gene expression6
- In this line, reporter gene assays with combinatorial treatment of 3i-1000 and the BET bromodomain inhibitor (+)-JQ1 demonstrated the cooperative role of GATA4 and BRD4 in the modulation of chamber-specific cardiac gene expression. (biomedcentral.com)
- Published results indicate that protein kinase C (PKC) activity in conjunction with innervation-induced activation of cell signaling mediated by the muscarinic acetylcholine receptor, Gaq, and the 1,4,5 inositol triphosphate receptor 1 (IP3R1) differentially regulate fast versus slow muscle fiber type gene expression in innervated muscle fibers. (rosalindfranklin.edu)
- This work is primarily undertaken using cardiac, skeletal and smooth muscle cells and neurons as model systems, and it is aimed at understanding the role of specialized proteins that bind to DNA to orchestrate gene expression. (yorku.ca)
- MicroRNAs (miRNAs) are small noncoding RNAs (~22 nucleotides in length) that regulate gene expression post-transcriptionally by imperfect binding to the 3′ untranslated region of target mRNAs in a wide variety of cell types. (biomedcentral.com)
- Cardiac hypertrophy and failure are characterized by transcriptional reprogramming of gene expression. (ca.gov)
- Here we show that Brg1, a chromatin-remodelling protein, has a critical role in regulating cardiac growth, differentiation and gene expression. (ca.gov)
Signaling pathway2
- Second, it results in the modulation of the Wnt signaling pathway that governs cell differentiation. (shinshu-u.ac.jp)
- The notch signaling pathway, a regulatory mechanism for cell growth and differentiation, plays broad roles in several aspects of cardiac development. (imedpub.com)
Stem cell7
- Aptamers are single-stranded pieces of DNA that bind to target proteins and are able to modulate signaling cascades during cell differentiation when a stem cell commits to a specific functional role or phenotype. (shinshu-u.ac.jp)
- As such, stem cell therapy cannot be regarded as a valid therapeutic option for patients with cardiovascular disease in the present era of evidenced-based medicine, yet it holds great hope for the future. (escardio.org)
- Preclinical and some small-scaled clinical trials have suggested feasibility and safety of cardiac stem cell therapy. (escardio.org)
- Repair of scar tissue constitutes a challenge for cardiac stem cell therapy due to lack of adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
- It is widely used in biomedical research, mainly for studying developmental biology, stem cell biology, cell differentiation, and drug screening. (cytion.com)
- The recent publication in Stem Cell Research and Therapy by Wagh and colleagues reports a novel regulatory role for miR-363 in cardiomyocyte specification. (biomedcentral.com)
- This commentary discusses the findings from Wagh and colleagues published in this issue of Stem Cell Research and Therapy demonstrating a critical role for miR-363 in post-transcriptional regulation of CM differentiation via the hand and neural crest derivative expressed HAND1 transcription factor [ 1 ]. (biomedcentral.com)
Maturation8
- However, reshaping of the epigenome of these terminally differentiated cells during fetal development, postnatal maturation, and in disease remains unknown. (nature.com)
- We find that prenatal development and postnatal maturation are characterized by a cooperation of active CpG methylation and histone marks at cis -regulatory and genic regions to shape the cardiac myocyte transcriptome. (nature.com)
- This study uncovers distinct layers of epigenetic regulation not only during prenatal development and postnatal maturation but also in diseased human cardiac myocytes. (nature.com)
- However, the detailed epigenetic processes involved in maturation from fetal to adult CMs and in cardiac disease leading to terminal heart failure have not been fully uncovered, yet. (nature.com)
- Purification of CM nuclei by fluorescence-assisted sorting has led to the identification of cell-type-specific mCpG and histone modification signatures in CMs during mouse heart development and maturation 11 . (nature.com)
- Here, we demonstrate that establishment of A/B compartments precedes and defines DNA methylation signatures during differentiation and maturation of cardiac myocytes. (galaxyproject.org)
- Recently, we have shown that pre-cardiac mesodermal cells plated on top of these stiffening HA hydrogels improves cardiomyocyte maturation compared to static, compliant polyacrylamide (PA) hydrogels [3]. (silverchair.com)
- While active mechanosensing causes maturation, the specific mechanisms responsible for responding to time-dependent stiffness remain unknown. (silverchair.com)
Proteins6
- TLRs are membrane-anchored proteins present in several cell types ranging from macrophages and T and B cells to nonimmune cells such as cardiomyocytes [ 20 , 21 ]. (hindawi.com)
- Because of the low abundance of most native ion channel proteins in mammalian cells, the purified native ion channel complexes are commonly contaminated by an overwhelming number and/or amount of non-specifically co-purified proteins, which makes the downstream mass-spectrometric and functional analyses very difficult. (mdanderson.org)
- Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
- Because of the difficulties of generating conformational mAbs against complex and highly conserved membrane proteins, no reliable tools exist to measure GLUT4 at the cell surface, follow its trafficking, or detect the conformational state of the protein. (pubchase.com)
- Single-chain formats of TNF-related apoptosis inducing ligand (scTRAIL) can serve as effector components of tumour-associated antigen-targeted as well as non-targeted fusion proteins, being characterized by high tumour cell-specific induction of apoptosis through death receptor activation. (pubchase.com)
- Mutations in these proteins may alter the function of renal cilia, which enable tubular cells to sense flow rates. (msdmanuals.com)
Dysfunction4
- During aging, gradual dysfunction of mitochondria (the powerhouses of the cells) and resulting oxidative damage are commonly observed. (usda.gov)
- Conclusion: Astrocytic dysfunction leading to synaptic dysfunction reported previously in AD may be a common pathogenic mechanism underlying both AD and DMD, linking protein alterations to cognitive impairment. (bvsalud.org)
- Diabetic cardiomyopathy is defined as a ventricular dysfunction initiated by alterations in cardiac energy substrates in the absence of coronary artery disease and hypertension. (hindawi.com)
- During myocardium infarction or heart failure, the extensive loss of CMs leads to progressive cardiac dysfunction and chronic heart failure, a leading cause of morbidity and mortality in the developed world. (biomedcentral.com)
Induces2
- Induces the differentiation of human PSC-derived alveolar epithelial type II (AETII) to AETI cells (Ghaedi et al. (stemcell.com)
- Retinoic acid induces the development of neurons, microglia, and astroglia, whereas DMSO initiates beating cardiomyocytes and smooth muscle cell development from P19 cells. (cytion.com)
Precursor cells2
- Next, we show that the proepicardium, which provides the precursor cells required for epicardial and coronary vessel development, migrates to the developing heart via direct contact of multicellular proepicardial villi to the developing myocardium. (arizona.edu)
- One complementary therapy could be 're-activating' vasculogenesis (i.e. the differentiation of precursor cells into mature endothelial cells), a mechanism that occurs in the heart during development but is quiescent in adult hearts. (elifesciences.org)
Embryogenesis1
- The HOX family of genes plays a major role in embryogenesis and cell differentiation, including differentiation of hematopoietic cell lines. (medscape.com)
IPSCs2
- Guiding iPSCs toward a target cell line has garnered much attention, but the process remains challenging. (shinshu-u.ac.jp)
- His lab uses human iPSCs, cardiac directed differentiation, genome editing (CRISPR), multi-omics, 3D organoids and engineered heart tissues as developmental and disease models. (armi.org.au)
Epigenetic3
- Epigenetic mechanisms and transcription factor networks essential for differentiation of cardiac myocytes have been uncovered. (nature.com)
- Epigenetic mechanisms are highly cell-type-specific requiring cell separation techniques to determine epigenomic features in a specific cell type, especially when keeping in mind that the cellular composition of the human heart is highly dynamic. (nature.com)
- Thus, different layers of chromatin organization and epigenetic control mechanisms exist. (galaxyproject.org)
Genes5
- A number of genes have been associated with cardiac manifestations. (wikipedia.org)
- Our on-going research is focused on the regulatory mechanisms that link innervation, PKC activity, IP3R1 activity, transcriptional regulators, and expression of slow muscle fiber type specific genes. (rosalindfranklin.edu)
- During vertebrate myogenesis, myoblast cell populations proliferate and fuse to form multinucleated muscle fibers that express a battery of contractile protein genes. (rosalindfranklin.edu)
- The mechanisms that regulate myoblast cell proliferation versus differentiation involve extracellular signaling via growth factors, their cognate receptors, intracellular signal transduction cascades, and transcriptional regulation of genes associated with cell proliferation. (rosalindfranklin.edu)
- Among these genes is the fibroblast growth factor receptor 1 (FGFR1) gene which is expressed in proliferating myoblasts and down-regulated during differentiation. (rosalindfranklin.edu)
Promotes3
- The formation of new vessels in the hypoxic area restores blood flow, provides oxygen and nutriments to the surviving cells, and promotes the migration and engraftment of new cells. (elifesciences.org)
- Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment. (rndsystems.com)
- Vasopressin promotes cell growth and fluid secretion via the cyclic AMP pathway, which leads to increase in the size and number of cysts in polycystic kidney disease. (msdmanuals.com)
Regulates3
- Notch signaling regulates cell-fate determination during activation by signal sending and receiving, affected through ligand-receptor crosstalk. (frontiersin.org)
- Results suggest that TRP14 regulates osteoclasts differentiation and bone resorption through its catalytic activity and that enhancing TRP14 may present a new strategy for preventing bone resorption diseases. (nih.gov)
- Poison Exon Splicing Regulates a Coordinated Network of SR Protein Expression during Differentiation and Tumorigenesis. (rndsystems.com)
Fibrosis4
- Atherosclerotic plaque rupture is the leading cause of cardiovascular death resulting from acute coronary syndrome (ACS) and leads to cardiac remodeling and fibrosis following ACS. (archivesofmedicalscience.com)
- A cardiac fibrosis model was established in Sprague-Dawley rats through a subcutaneous injection of isoproterenol (ISO, 5 mg/kg/d). (dovepress.com)
- TMZ may ameliorate EndMT and ISO-induced cardiac fibrosis through the NOX2/NF-κB/Snail pathway. (dovepress.com)
- The findings of the study may provide new insights into the potential role of TMZ in the pathophysiology of cardiac fibrosis. (dovepress.com)
Tissue3
- Owing to technical restrictions, these studies were performed in heart tissue and therefore the affected cell type(s) could not be identified. (nature.com)
- Studies have shown that cell-to-cell mitochondrial transfer plays an essential role in regulating cardiovascular system development and maintaining normal tissue homeostasis under physiological conditions. (frontiersin.org)
- Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
Lineages1
- These unique clones allow for to study into specific epicardial cell lineages and phenotypes. (arizona.edu)
Regulation2
- Collectively our results provide significant advancements in our understanding of EMT regulation during cardiac development. (arizona.edu)
- Ion channels are membrane protein complexes that translocate ions across cell or organelle membranes, underlying a broad range of the most basic physiological processes from nerve and muscle excitability, to membrane potential setting, pH/cell volume regulation, secretion and absorption. (mdanderson.org)
Protein4
- Mitochondria not only serve as power plants in cells but also act as crucial regulators in many biological processes, including reactive oxygen species (ROS) signaling, redox balance, calcium homeostasis, protein quality control, and programmed cell death ( 3 , 4 ). (frontiersin.org)
- induced cardiac toxicity such as oxidative stress and deteriorated mitochondrial function, a disturbance in iron regulatory protein [(IRP)-1], the release of nitric oxide, autophagy and dysregulation of Ca 2+ level. (mosuljournals.com)
- The proximal events of TLR-mediated intracellular signaling are initiated by interactions with cytosolic adapters, mainly myeloid differentiation primary response protein 88 (MyD88) [ 25 ]. (hindawi.com)
- Using these cell culture systems we have set up multiple screening assays using iPSC-CMs such as lipid droplets, protein aggregation, and mitochondrial function and functional pathophysiological screenings for calcium handling and contractility. (sluijterlab.com)
Regenerative2
- Unfortunately, regenerative medicine is not yet feasible outside a laboratory setting as available protocols to generate target cells are complicated and expensive. (shinshu-u.ac.jp)
- Understanding their mechanisms may lead to the development of microRNA cocktails that can potentially be used in regenerative cardiology. (archivesofmedicalscience.com)
Cardiovascular4
- Notably, cis -regulatory regions in cardiac myocytes are significantly enriched for cardiovascular disease-associated variants. (nature.com)
- In this review, we summarized the mechanism of mitochondrial transfer in the cardiovascular system and outlined the fate and functional role of donor mitochondria. (frontiersin.org)
- We also discussed the advantage and challenges of mitochondrial transfer strategies, including cell-based mitochondrial transplantation, extracellular vesicle-based mitochondrial transplantation, and naked mitochondrial transplantation, for the treatment of cardiovascular disorders. (frontiersin.org)
- Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
Mitochondrial function2
- The results showed that older had lower Nrf2 activity and mitochondrial function, together with a lower skeletal muscle mass and cardiac function compared to young mice. (usda.gov)
- In the old mice, SFN restored Nrf2 activity, mitochondrial function, cardiac function, capacity to exercise, handling of sugar, and activation of skeletal muscle satellite cells (important for renewal of the muscle cells). (usda.gov)
Fetal3
- DMEM/Ham's F12 media containing 5% Fetal bovine serum, 3.1 g/L Glucose, 1.6 mM L-Glutamine, 1.0 mM Sodium pyruvate, 15 mM HEPES and 1.2 g/L NaHCO3 is used to culture P19 cells. (cytion.com)
- Cardiac stress triggers adult hearts to undergo hypertrophy and a shift from alpha-MHC to fetal beta-MHC expression. (ca.gov)
- It preserves fetal cardiac differentiation by interacting with histone deacetylase (HDAC) and poly (ADP ribose) polymerase (PARP) to repress alpha-MHC and activate beta-MHC. (ca.gov)
Inflammation3
- Littman, D. R. & Rudensky, A. Y. Th17 and regulatory T cells in mediating and restraining inflammation. (nature.com)
- Cardiac Toll-like receptors and inflammasome complexes may be key inducers for inflammation probably through NF- B activation and ROS overproduction. (hindawi.com)
- Activation of Toll-like receptors (TLRs) and the inflammasome complex has recently been proposed to be central in cardiac inflammation and likely in the pathogenesis of DCM. (hindawi.com)
Biology1
- Fabrice Jaffré is an Assistant Professor of Cell and Developmental Biology at Weill Cornell Medical College in New York City. (armi.org.au)
Therapy4
- Cell therapy is a potential new treatment for post MI patients, but the main challenges are : the necessity to preserve immune competency and to gain adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
- Cell therapy is currently emerging as a potential new treatment for post MI patients with the assumption that recolonization of the areas of scarred myocardium with exogenously supplied surrogates or precursors of cardiomyocytes can restore function and ultimately affect clinical outcomes. (escardio.org)
- These studies have established the basis for future PSC-based cardiac therapy. (ucla.edu)
- Further exploring the role of miRNAs in cardiac cells during development and disease may therefore hold great promise for cardiac therapy applications. (biomedcentral.com)
Fibroblast1
- A subset of epicardial cells undergoes EMT, migrates into the developing heart, and differentiates into cardiac fibroblast, vascular endothelial, and smooth muscle cells. (arizona.edu)
HESCs1
- To this end, the authors performed miRNA expression profiling in undifferentiated hESCs and CMs at day 8 and day 14 after differentiation. (biomedcentral.com)
Coronary3
- in 1971 [ 2 ], diabetic cardiomyopathy (DCM) is characterized by the direct effect of diabetes on cardiac structure and function in the absence of coronary artery disease, hypertension, or other cardiac pathologies. (hindawi.com)
- HN - 2008 BX - Granulosa Cells, Cumulus MH - Coronary Sinus UI - D054326 MN - A07.231.908.194.500 MS - A short vein that collects about two thirds of the venous blood from the MYOCARDIUM and drains into the RIGHT ATRIUM. (bvsalud.org)
- Coronary sinus, normally located between the LEFT ATRIUM and LEFT VENTRICLE on the posterior surface of the heart, can serve as an anatomical reference for cardiac procedures. (bvsalud.org)
Function8
- Here we present SR1001, a high-affinity synthetic ligand-the first in a new class of compound-that is specific to both RORα and RORγt and which inhibits T H 17 cell differentiation and function. (nature.com)
- Our data demonstrate the feasibility of targeting the orphan receptors RORα and RORγt to inhibit specifically T H 17 cell differentiation and function, and indicate that this novel class of compound has potential utility in the treatment of autoimmune diseases. (nature.com)
- More detailed structure-function analysis revealed a distinct subclass of GATA4/NKX2-5 inhibitory compounds with an acetyl lysine-like domain that contributed to ventricular cells (%Myl2-eGFP+). (biomedcentral.com)
- Given the complexity of the biological function of mitochondria, researchers have begun to consider rescuing the injured cells through mitochondrial transfer, that is, replacing damaged mitochondria with healthy mitochondria from donor cells. (frontiersin.org)
- Our studies revealed a significant drop in Nrf2 activity and mitochondrial functions, together with a loss of skeletal muscle and cardiac function in the old control mice compared to the younger age group. (usda.gov)
- Through this mechanism, a single microRNA can alter the function of multiple mRNAs. (archivesofmedicalscience.com)
- The gene is active in these cells for only a short period of time as they migrate toward the heart and dorsal aorta, where they give rise to blood stem cells buy 349085-38-7 These findings suggest that plays a significant function in triggering developmental procedures that eventually bring about arteries and blood cellular material. (baxkyardgardener.com)
- 2) T cell deficiencies affect the function in killing infected cells or helping other immune cells. (lu.se)
Differentiate1
- Our results demonstrated that these immature cells have a natural capacity to differentiate into endothelial cells in infarcted hearts. (elifesciences.org)
Human umbilical vein endothelial cells1
- We show that primary human umbilical vein endothelial cells (HUVECs) readily uptake the nanoparticles with and without specific antibody targets. (frontiersin.org)
Functional3
- They demonstrated that transferring functional mitochondria of bone marrow-derived stem cells to defective parenchymal cells increases the aerobic respiration capacity of recipient mitochondria ( 7 ). (frontiersin.org)
- The study of promoter interactions in other hESC-derived cell types may be of utility in functional investigation of GWAS-associated regions. (manchester.ac.uk)
- Macrophage differentiation into functional phenotypes influences the fate of nanomaterials taken up by these immune cells. (cdc.gov)