Loading...
  • polymerases
  • DNA polymerases isolated from cells and artificial DNA primers can be used to initiate DNA synthesis at known sequences in a template DNA molecule. (wikipedia.org)
  • fungi
  • The fact that the basic mechanisms involved in the aging process are believed to be universal allows the use of different model systems, from the simplest eukaryotic cells such as fungi to the most complex organisms such as mice or human. (hindawi.com)
  • inhibitors
  • Although the great amount of pre-clinical and clinical data are from the solid tumor experience, only few studies have been done on leukemias using specific cell cycle checkpoint inhibitors. (biomedcentral.com)
  • This review, for the first time, outlines the most important pre-clinical and clinical data available on the efficacy of cell cycle checkpoint inhibitors in single agent and in combination with different agents normally used for the treatment of acute and chronic leukemias. (biomedcentral.com)
  • complex
  • The formation of the cyclin E-CDK2 complex then promotes a positive feedback loop which creates an "all or nothing" switch from which the cell can not return. (wikipedia.org)
  • Trillions of cell divisions subsequently occur in a controlled manner to produce a complex, multicellular human. (lumenlearning.com)
  • Mad1 is phosphorylated by Mps1 which then leads together with other activities to the formation of the mitotic checkpoint complex (MCC). (wikipedia.org)
  • This second c-MAD2 is transferred to Cdc20 with yet unknown mechanism and forms Cdc20-c-Mad2 complex. (wikipedia.org)
  • This complex is an essential component of mitotic checkpoint complex (MCC). (wikipedia.org)
  • The complex network of biochemical reaction/transport processes and their spatial organization make the development of a predictive model of a living cell a grand challenge for the 21st century. (wikipedia.org)
  • The eukaryotic cell cycle is very complex and is one of the most studied topics, since its misregulation leads to cancers. (wikipedia.org)
  • This finding suggests that key components of the eukaryotic MRN complex are derived by evolutionary descent from the archaea. (wikipedia.org)
  • However, upregulation of MRN complex sub-units has been documented in certain cancer cell lines when compared to non-malignant somatic cells, suggesting some cancer cells have developed a reliance on MRN overexpression. (wikipedia.org)
  • Since tumor cells have increased mitotic rates compared to non-malignant cells this is not entirely unexpected, as it is plausible that an increased rate of DNA replication necessitates higher nuclear levels of the MRN complex. (wikipedia.org)
  • Although the causal relationship between GCN2 and cell cycle delay is still under debate, it was suggested that the formation of the pre-replication complex is deferred by GCN2 upon UV-irradiation. (wikipedia.org)
  • Eukaryotic DNA has a complex structure. (wikipedia.org)
  • flow cytometry
  • Below each Western is the accompanying flow cytometry following the cell cycle progression by DNA content for each experiment. (genetics.org)
  • Percentage of undivided nuclei was scored by flourescence microscopy from cells stained for flow cytometry. (genetics.org)
  • We followed DNA content by flow cytometry and nuclear morphology as independent measures of cell cycle progression. (genetics.org)
  • Cells released into galactose ( Figure 1A , bottom) progressed through G 2 /M as shown by flow cytometry, divided their nuclei and began to accumulate in G 1 of the next cell cycle in the late stages of the time course. (genetics.org)
  • regulator
  • To address this issue, we have undertaken a functional analysis of Drosophila Myt1 that has revealed novel developmental roles for this conserved cell cycle regulator during gametogenesis. (biologists.org)