• and 4) synthesis of oligo-saccharides and drug-glycoconjugates (these drugs include antitumor agents such as camptothecin, taxol, and etoposide) for targeted drug delivery. (umich.edu)
  • Our laboratory has over 20 years of experience working with these important bacterial pathogens and has gained significant expertise in areas relating to Burkholderia genetics, pathogenesis, host-pathogen interactions, carbohydrate purification/characterization, glycoconjugate synthesis and the analysis of humoral/cellular immune responses. (unr.edu)
  • After induction glycoconjugates generally appeared later than unglycosylated carrier protein, suggesting that glycosylation was the rate-limiting step for synthesis of conjugate vaccines in E. coli . (biomedcentral.com)
  • Glycoconjugate synthesis, in particular expression of oligosaccharyltransferase PglB, strongly inhibited growth of E. coli cells after induction, making it necessary to separate biomass growth and recombinant protein expression phases. (biomedcentral.com)
  • A detailed understanding of the combining site of this antibody and the measurement of its binding to different ligands is of interest as this knowledge will have implications in the characterization of arabinofuranose-containing glycoconjugates that are increasingly recognized as important biological molecules. (uni-luebeck.de)
  • The global effort is modeled on the Human Genome Project and aims to map the structures and functions of human glycoconjugates, or glycans, oligosaccharides covalently attached to the backbone of proteins. (genos-glyco.com)
  • Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. (biotrend.com)
  • Of even greater significance is that an in-depth study of this carbohydrate-protein interaction will provide insights into the mechanisms by which oligosaccharides containing furanose rings are bound by proteins, an area that has, to date, received little attention. (uni-luebeck.de)
  • Breast milk also contains other immunoglobulins, antibodies, oligosaccharides, lipids, bioactive peptides, among other components with unique mechanisms that besides the protection against these diseases, stimulate the development of infants' immune systems. (bvsalud.org)
  • This study was designed to identify signaling pathways and crucial proteins involved in the pathological mechanism of KBD compared with osteoarthritis (OA). (nature.com)
  • Golgi resident proteins, such as glycosyltransferases and sugar nucleotide transporters, are precisely distributed across the Golgi stacks by recycling mechanisms that counteract the flow of ongoing vesicular transport. (cornell.edu)
  • Lectins are proteins or glycoproteins that bind specifically and reversibly to the carbohydrate or glycoconjugates. (bvsalud.org)
  • The Golgi complex plays a prominent role in secretory and endocytic trafficking in eukaryotic cells and is key to the biosynthesis of glycoconjugates (glycoproteins and glycolipids) that are essential for life. (cornell.edu)
  • The histological analysis showed that the MPLE dietary supplementation promoted an increase in the number of intestinal goblet cells and modified the composition of mucins' glycoproteins stored in goblet cells, with an increase in the staining intensity of neutral mucins, as well as in mucins rich in carboxylated and weakly sulfated glycoconjugates, particularly those rich in sialic acid residues. (nih.gov)
  • Glycoconjugates (glycoproteins, glycolipids, proteoglycans) are sugar-containing molecules that have many important structural and modulatory functions in biological systems (13). (9lib.net)
  • Treatment of cells with inhibitors of these enzymes results in the production of glycans or glycoconjugates containing missing or altered glycan chains. (go.jp)
  • This approach is highly useful for examining the potential functional role(s) of glycans and glycoconjugates in cells or tissues, and in biological processes of health and disease. (go.jp)
  • Together, free HMOs and their related glycoconjugates have been referred to as human milk glycans (HMGs) [ 6 ]. (biomedcentral.com)
  • The goals of this workshop were to examine the mechanisms involved in generating an appropriate immune response to selected carbohydrate antigens, highlight recent and novel advances, and discuss how this information could be used in the development of effective vaccines. (cdc.gov)
  • The meeting was organized into 7 sessions on such topics as genetic and cellular mechanisms of carbohydrate immunity, carbohydrate antigens for vaccines, and new tools for studying carbohydrates. (cdc.gov)
  • Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
  • In the present work, effects in the gastric mucosa of mice treated with Pteridium aquilinum were evaluated, as well as molecular mechanisms underlying the synergistic role with Helicobacter pylori infection. (pasteur.fr)
  • The gene transcription alterations and the induced glycophenotypic changes observed in the gastric mucosa contribute for the understanding of the molecular mechanisms underlying the role of Pteridium aquilinum in the gastric carcinogenesis process. (pasteur.fr)
  • Cisplatin in cancer therapy: molecular mechanisms of action. (innovareacademics.in)
  • The biominerals are natural nano-composites of protein-crystal interactions and the molecular mechanisms underlying the biomineral formation have inspired nanotechnology applications by virtue of their bottom-up approach [5] [6]. (cancer-ecosystem.com)
  • However Speer3 the detailed molecular mechanisms of biomineralization still remain to be mysterious. (cancer-ecosystem.com)
  • Research in the Brett laboratory is focused on elucidating the molecular mechanisms used by Burkholderia pseudomallei (melioidosis) and Burkholderia mallei (glanders) to evade clearance by host defenses. (unr.edu)
  • However little is known about the molecular mechanisms governing the glycosylation shift that occurs in gastric mucosa in response to illness. (bio-cavagnou.info)
  • Lectin binding studies suggest that membranes of hypolemmal cisternae contain glycoconjugates, similar to plasma membrane. (grantome.com)
  • Several studies have shown the functions of tympanic membranes and the syringeal muscles of the syrinx for sound-generating mechanisms in birds (2-7). (9lib.net)
  • Dysfunction in these mechanisms, or their hijacking by viruses and toxins, is known to have serious consequences for human health, leading to congenital disorders of glycosylation, cancers, and immune dysfunction. (cornell.edu)
  • However the activation of this glycosylation pathway as well as the living of additional regulatory mechanisms underlying these glycophenotypic changes in the complex context of individual chronic infection haven't been attended to. (bio-cavagnou.info)
  • Research programs in our group aim at the understanding of the mechanism of action of small organic molecules that elicit significant biological responses upon their interactions with biological macromolecules. (umich.edu)
  • These organic molecules include active forms of carcinogenic polycyclic aromatic hydrocarbons (PAHs), DNA-cleaving natural products, and glycoconjugate molecules. (umich.edu)
  • it is a normal constituent of plasma and degrades a wide range of glycoconjugates containing β-linked N -acetylhexosaminyl residues. (medscape.com)
  • We demonstrated a differential anti-αGal isotype inhibition as a function of the length of the poly-L-lysine and the number of αGal residues exposed in the glycoconjugates. (gencat.cat)
  • The gut transcripteractome revealed an expression profile that favored biological mechanisms associated to the 1) immune system, particularly involving T cell activation and differentiation, 2) gut integrity (i.e., adherens and tight junctions) and cellular proliferation, and 3) cellular proteolytic pathways. (nih.gov)
  • CTB is also proven to possess neuroprotective mechanisms, contribute to the maintenance of intestinal homeostasis, induce wound-healing effects, and improve the efficiency of drug delivery and treatment of inflammatory diseases. (mdpi.com)
  • My lab is interested in uncovering these fundamental mechanisms governing Golgi homeostasis using a multifaceted approach combining genetics, flow-cytometry, imaging, in vitro reconstitution, and proteomics. (cornell.edu)
  • Moreover, an animal model for αGal sensitization in GalT-KO mice was developed by intradermal administration of hard tick' salivary gland extract, mimicking the sensitization mechanism postulated in humans. (gencat.cat)
  • Whilst Bt protein toxins are heavily utilised, the exact mechanisms behind receptor binding and toxicity are unknown. (cardiff.ac.uk)
  • This work presents an immunotherapy based on polymeric αGal-glycoconjugates for potentially treating allergic disorders by selectively inhibiting anti-αGal IgE antibodies. (gencat.cat)
  • Our results showed that exposure to Pteridium aquilinum induces histomorphological modifications including increased expression of acidic glycoconjugates in the gastric mucosa. (pasteur.fr)
  • The objective of this study was to characterize chemical properties of water-soluble extract (WSE) obtained from yeast cell wall, and study its antibacterial activity and antimicrobial mechanisms against E. coli . (springeropen.com)
  • This work provides more information, regarding chemical properties and anti- E. coli mechanism of WSE, and facilitates the application of yeast cell wall products as a promising antibiotic alternative in animal feed. (springeropen.com)
  • Cell Surface and Extracellular Glycoconjugates: Structure and Function. (wikipedia.org)
  • Our findings have opened doors for interrogating new players and dissecting the mechanisms critical to maintain Golgi identity and function. (cornell.edu)
  • Here, we provide evidence for a function of the GL-Lect mechanism in transcytosis across enterocytes in the mouse intestine. (institut-curie.org)
  • In this issue of Cell Host & Microbe, Clarke and colleagues uncover a coherent mechanism where the systemic spread of Firmicutes cell wall glycoconjugates enhances global immune fitness while simultaneously being delicately controlled to prevent systemic inflammation. (x-mol.com)
  • Further investigation of their regulative roles and interaction may provide new insights into the pathological mechanisms and therapeutic targets for KBD. (nature.com)
  • Umbilical cord blood cells have therapeutic potential for neurological disorders, through a paracrine mechanism of action. (cn1699.cn)
  • In some epithelial cells, which morphologically and functionally polarize in relation to different behaviours in ion transport [ 9 ], it is likely that a functional specialization of the glycoconjugates is observed on the basolateral surface. (hindawi.com)
  • Inside enterocytes, Gal3 is localized to hallmark structures of the GL-Lect mechanism, termed clathrin-independent carriers. (institut-curie.org)
  • These data pioneer the existence of GL-Lect endocytosis in vivo and strongly suggest that polarized trafficking across the intestinal barrier relies on this mechanism. (institut-curie.org)
  • Viral strategies to lyse host microbes can sometimes involve just a single gene, although the mechanism of action can be hard to discern. (x-mol.com)
  • The purpose of this study was to evaluate the extent and mechanism anti-cancer drug-loaded liposomes using wheat germ agglutinin as a guiding molecule. (innovareacademics.in)
  • The glycoconjugates, which are essential components of the glycocalyx of many cell types, take part in many functions, including osmoregulation, cell to cell recognition, binding of hormones, protection of cells from phagocytosis and dehydration, differentiation, defense, and ion transport [ 8 ]. (hindawi.com)
  • This family of sulphated glycoconjugates which are active against rosettes is also known to inhibit sporozoite invasion of hepatocytes and merozoite reinvasion of erythrocytes, suggesting that sulphated glycoconjugate interaction may be an important process in cell adhesion at different stages in the plasmodial life cycle. (ox.ac.uk)
  • These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. (biotrend.com)
  • Starting from shake flask cultivation in standard complex medium a lab-scale fed-batch process was developed for glycoconjugate production. (biomedcentral.com)
  • The aim of this study was to characterize the nature and regional distribution of the glycoconjugates secreted by epidermal mucous cells in Eisenia foetida (Annelida). (hindawi.com)
  • Histochemical staining properties of glycoconjugates in epidermal mucous cells were determined regionally. (hindawi.com)
  • The major goal of the proposed research is to elucidate mechanisms that underlie transport of membranous organelles that are related to plasma membrane renewal (i.e., recycling) in axons. (grantome.com)
  • In this paper we show that a group of sulphated glycoconjugates including sulphatide, dextran sulphate, and fucoidan are more effective rosette reversing agents than heparin and are active against both laboratory strains and wild isolates. (ox.ac.uk)
  • Mechanisms for sugar recognition have evolved independently in diverse protein structural frameworks, but share some key features. (embl.de)
  • This study provided comprehensive information about the regional distribution patterns of the glycoconjugates and an opportunity to compare their distributional patterns in other annelids. (hindawi.com)
  • Our study suggests the potential clinical use of poly-L-lysine-based αGal-glycoconjugates for treating allergic disorders mediated by anti-αGal IgE antibodies. (gencat.cat)
  • The mechanism of rosetting remains unknown, but the ability of heparin to disrupt rosettes has been recognised previously. (ox.ac.uk)