Measured measures cell cycle. Medical search. Frequent questions

Most studies have been performed on large cell populations, but detailed understanding of cell dynamics and heterogeneity requires single-cell analysis. (frontiersin.org)
In addition, in order to phenotypically anchor the gene expression data, changes in the heterogeneity of cell subtype populations and cell cycle phase were monitored using flow cytometry. (plos.org)

The purpose of this study was to determine the acute and long-term effects of mitomycin C (MMC) on quiescent rabbit corneal keratocytes regarding cell proliferation, myofibroblast differentiation and DNA repair. (molvis.org)
MMC significantly blocked TGFβ-induced cell proliferation and myofibroblast differentiation in cultured quiescent keratocytes and altered the transcriptional regulation of macrophage chemotactic protein-1 (MCP-1) and alpha smooth muscle actin (αSMA). (molvis.org)
Cancer stem cells are a selective clonal subset of tumour cells that have avoided various cell regulatory mechanisms, including terminal differentiation, and yet have retained the self-renewal properties and proliferative potential of adult stem cells. (who.int)

Cell proliferation includes a series of events that is tightly regulated by several checkpoints and layers of control mechanisms. (frontiersin.org)
In conclusion, we provide useful experimental approaches and bioinformatics to identify informative and predictive genes at the single-cell level, which opens up new means to describe and understand cell proliferation and subpopulation dynamics. (frontiersin.org)
However, most of our knowledge about cell proliferation comes from studies that average data from large and mixed cell populations. (frontiersin.org)

MMC also induced phosphorylation of the nuclear histone marker of DNA damage, γH2AX (a member of the H2A histone family), without induction of cell cycle entry or immediate DNA repair measured by Comet assay. (molvis.org)
A "one-cycle infection-based" assay measures how well baloxavir inhibits the early stages of influenza virus infection (at a single cell level), while a "multi-cycle infection-based" assay measures how well baloxavir blocks infection of neighboring healthy cells. (cdc.gov)
This multi-cycle assay is primarily used to test highly pathogenic avian influenza (HPAI) viruses with pandemic potential, whereas the one-cycle assay is used for testing of seasonal and other influenza viruses. (cdc.gov)
In NHANES 2011-2012, population folate status was assessed by a combination of two analytical methods: whole-blood folate was measured by microbiologic assay, while serum folate forms were measured by isotope-dilution high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). RBC folate was then calculated using the data from both assays. (cdc.gov)

Detailed analysis of cell cycle predictive genes allowed us to define subpopulations with distinct gene expression profiles and to calculate a cell cycle index that illustrates the transition of cells between cell cycle phases. (frontiersin.org)
Here, we employed single-cell gene expression profiling to describe the dynamic transition between cell proliferative states in three different cell lines using a panel consisting of 93 marker genes. (frontiersin.org)
This study explores the use of a simple human neurosphere-based in vitro model to characterise the pharmacological and toxicological effects of LiCl and VPA using gene expression changes linked to phenotypic alterations in cells. (plos.org)
These data indicate that the effect of MMC on corneal scarring and haze is related to the generation of DNA ICLs leading to defective cell replication and gene expression. (molvis.org)

The immunogenic subtype had enriched immune cells and could benefit from immune checkpoint therapy. (bvsalud.org)

Mantle cell lymphoma (MCL) is a lymphoproliferative disorder derived from a subset of naive pregerminal center cells localized in primary follicles or in the mantle region of secondary follicles. (medscape.com)

MMC induces DNA damage to quiescent corneal keratocytes, which remains unrepaired, resulting in abnormal cell replication and gene transcription that leads to long-term effects on corneal repair. (molvis.org)
In comparison, the other available flu antiviral medications, which are "neuraminidase inhibitors," do not directly interrupt replication within an infected cell, but instead work to prevent new copies of the virus from leaving the infected cell and spreading to healthy cells. (cdc.gov)

We found that the total transcript level per cell and the expression of most individual genes correlated with progression through the cell cycle, but not with cell size. (frontiersin.org)
WBV exposure resulted in a significant reduction in number of developing follicles, and decrease in circulating estradiol concentrations, ovarian luteinizing hormone receptor protein levels, and marked changes in transcript levels for several factors involved in follicular development, cell cycle, and steroidogenesis. (cdc.gov)

Laboratory assays also were developed to test the susceptibility of influenza viruses to baloxavir in cell cultures. (cdc.gov)

Whilst LiCl exposure did not significantly alter the proportion of cells expressing markers for stem cells/undifferentiated cells (Oct4, SSEA4), neurons (Neurofilament M), astrocytes (GFAP) or cell cycle phase, the drug caused a 1.4-fold increase in total cell number. (plos.org)
The effects of these exposures on hormone concentrations, and markers of function and/or injury in the olfactory bulb, hypothalamus and testes were measured after 1, 8 and 30days exposure. (cdc.gov)

Water 2H and 17O spin relaxation is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms. (lu.se)
There is no significant difference in cell water dynamics between mesophilic and halophilic organisms, despite the high K+ and Na+ concentrations in the latter. (lu.se)

This makes it possible to quantify and control the biodynamic responses conveniently and reliably by measuring the loading device response. (cdc.gov)

[ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)

The molecular processes behind cell cycle progression have been dissected by numerous morphological studies on live or fixed single cells using a plethora of techniques to visualize components and processes during cell division. (frontiersin.org)

In contrast, exposure to VPA resulted in significant upregulation of Oct4, SSEA, Neurofilament M and GFAP with significant decreases in both G2/M phase cells and cell number. (plos.org)

Human embryonic stem cells (hESc) can also be used to analyze the in vitro development from undifferentiated pluripotent cells leading to terminally differentiated cell types, recapitulating the process of early embryonic development [5] . (plos.org)

Many more investigations have been made on cells, sorted according to size, or artificially arrested at various cell cycle checkpoints. (frontiersin.org)

Analysis at the single-cell level can overcome most of these limitations. (frontiersin.org)

Treatment selection takes into account patient age, fitness, and whether autologous stem cell transplantation (ASCT) is planned. (medscape.com)

the folate level can be assessed by measuring the turbidity of the inoculated medium at 590 nm in a PowerWave X340 Microplate reader (Bio-Tek Instrument). (cdc.gov)

Folate forms are separated within 6 min using isocratic mobile phase conditions and measured by LC-MS/MS. Quantitation is based on peak area ratios interpolated against a five-point aqueous calibration curve. (cdc.gov)

In males, WBV resulted in a significant reduction in spermatids and circulating prolactin levels, elevation in number of males having higher circulating testosterone concentrations, and marked alterations in levels of transcripts associated with oxidative stress, inflammation, and factors involved in regulating the cell cycle. (cdc.gov)

By applying the random forests algorithm, a supervised machine learning approach, we show how a multi-gene signature that classifies individual cells into their correct cell cycle phase and cell size can be generated. (frontiersin.org)

Indeed, both the hESc and human neuroprogenitor cell (hNPC) models provide valid and useful tools for studying DNT. (plos.org)

Water exchange between the hydration layer and bulk water is invariably fast on the NMR time scale so the measured spin relaxation rate is a population-weighted average over these environments. (lu.se)

Water in living cells behaves as expected from studies of simpler model systems, the only difference being a larger fraction of secluded (strongly perturbed) hydration sites associated with the supramolecular organization in the cell. (lu.se)

Of particular interest is their contribution to Developmental Neurotoxicity (DNT) as well as their potential impact on stem cells. (plos.org)

Analyzing the data gives automakers insights into which lithium ion battery packs or cells are performing better than others and safety information about the condition and performance of the battery under different pressures. (autonews.com)

This means that it blocks the ability of the virus to make copies of itself within an infected cell. (cdc.gov)

it was frequently categorized as diffuse small-cleaved cell lymphoma (by the International Working Formulation) or centrocytic lymphoma (by the Kiel classification). (medscape.com)

It can measure voltage, for instance, and prevent the battery from being overcharged. (autonews.com)

Wash the cells by addition of 10 mL PBS. (mdanderson.org)

Here, we used quantitative real-time PCR, profiling the expression of 93 genes in single-cells from three different cell lines. (frontiersin.org)

Arbyn M, Bergeron C, Klinkhamer P, Martin-Hirsch squamous cell carcinoma in a high-risk region in Iran. (who.int)

Anatomy24

Organisms11

Diseases4

Chemicals and Drugs108

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Psychiatry and Psychology1

Phenomena and Processes81

Disciplines and Occupations2

Information Science5

Health Care11

Heterogeneity2

Differentiation3

Proliferation3

Assay4

Gene expression4

Subtype1

Subset1

Replication2

Transcript2

Cultures1

Markers2

Dynamics2

Responses1

Inhibitors1

Molecular1

Exposure1

Development1

Various cell1

Single-cell level1

Treatment1

Level1

Conditions1

Significant1

Phase1

Provide1

Population1

Studies1

Potential1

Data1

Blocks1

Small1

Prevent1

Addition1

Expression1

Region1