Maturation aurora kinases. Medical search. Frequent questions

Aurora kinase A also known as serine/threonine-protein kinase 6 is an enzyme that in humans is encoded by the AURKA gene. (wikipedia.org)
The Aurora kinase B is a chromosome passenger protein and regulates chromosome segregation and cytokinesis. (wikipedia.org)
Although there is evidence to suggest that Aurora C might be a chromosomal passenger protein, the cellular function of it is less clear. (wikipedia.org)
In the absence of Aurora A mad2, a protein that normally dissipates once a proper kinetochore-microtubule connection is made, remains present even into metaphase. (wikipedia.org)
Many eukaryotic protein kinases are activated by phosphorylation on a specific conserved residue in the regulatory activation loop, a post-translational modification thought to stabilize the active DFG-In state of the catalytic domain. (elifesciences.org)
This mechanism raises new questions about the functional role of the DFG-Out state in protein kinases. (elifesciences.org)
The enzymes that catalyze this process, called protein kinases, are themselves controlled by the phosphorylation of a flexible region called the activation loop. (elifesciences.org)
This assumption was based on static pictures of protein kinases obtained by X-ray crystallography, in which individual states are trapped and visualized in a crystal lattice. (elifesciences.org)
show that the static model is incorrect in a protein kinase called Aurora A. In this enzyme, the phosphorylated activation loop continues to switch back and forth between active and inactive states. (elifesciences.org)
Stringent regulatory control of protein kinases is critically important for the integrity of cellular signal transduction. (elifesciences.org)
The catalytic activity of protein kinases is regulated by finely-tuned allosteric mechanisms that reversibly switch the kinase domain between active and inactive conformational states ( Huse and Kuriyan, 2002 ). (elifesciences.org)
2. TACC1-chTOG-Aurora A protein complex in breast cancer. (nih.gov)
All of these members contain a conserved C-terminal amino acid sequence termed the polo box domain which provides a docking site for certain proteins (1) and a kinase domain which is predicted to activate many protein kinases including Aurora A/B PKA ERK1/2 RSK1/2 Akt/PKB and MEK1 (2). (biospraysehatalami.com)
You can find six phosphorylation sites in RSK1 that are crucial for its activation and its own subsequent part in substrate phosphorylation (21 22 Of the phosphorylation of Ser-380 can be very important to RSK1 activation traveling functions such as for example rules of gene manifestation and protein synthesis and cell cycle regulation as a downstream Nisoxetine hydrochloride kinase in the Mos-MAPK pathway (23). (biospraysehatalami.com)
Interestingly, Tripolin A affected the gradient distribution towards the chromosomes, but not the MT binding of HURP (Hepatoma Up-Regulated Protein), a MT-associated protein (MAP) and substrate of the Aurora A kinase. (forumbcn2004.org)
3) chemical inducers primarily phosphatase inhibitors such as okadaic acidity calyculin A and fostreicin.14-18 In the second option case activation from the maturation/mitosis promoting element (MPF) is an integral mediator of PCC while Cdk1 (p34cdc2) bound Caudatin to cyclin B1 is activated by tyrosine dephosphorylation by cdc25c.19 Cdc25 itself is controlled by auto-phosphorylation and dephosphorylation by protein phosphatases PP2A or PP1. (monossabios.com)
Advance your cancer research with our high-quality Recombinant Human SRC protein, also known as Proto-oncogene tyrosine-protein kinase Src and Proto-oncogene c-Src. (cusabio.com)
The DPY19L2 protein is involved in the development of the acrosome and elongation of the sperm head, which are integral steps in sperm cell maturation. (medlineplus.gov)
Protein kinase C zeta isoform is critical for proliferation in human glioblastoma cell lines. (ucdenver.edu)
Microvesicles loaded with MCs encoding a thymidine kinase (TK)/nitroreductase (NTR) fusion protein produced prolonged TK-NTR expression in mammary carcinoma cells. (regenerativemedicine.net)
Serine-threonine/tyrosine-protein kinase, catalytic domain [Interproscan]. (ntu.edu.sg)
Essential role of protein kinase C zeta in transducing a motility signal induced by superoxide and a chemotactic peptide, fMLP. (labome.cn)
B-raf belongs to the the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which may be constitutively activated due to BRAF gene mutations. (blogspot.com)
Protein kinases that control cell cycle progression in all eukaryotes and require physical association with CYCLINS to achieve full enzymatic activity. (nih.gov)
A giant elastic protein of molecular mass ranging from 2,993 kDa (cardiac), 3,300 kDa (psoas), to 3,700 kDa (soleus) having a kinase domain. (lookformedical.com)
Protein kinase affinity probe 1 is a novel protein kinase affinity probe for the functional identification of protein kinases (PKs) . (medchemexpress.com)
Protein kinase affinity probe 1 is a modified Purvalanol B (HY-18299) probe with 50% beads loading (Compound S3). (medchemexpress.com)
PknB-IN-2 (Compound 10) is a Mycobacterium tuberculosis protein kinase B ( PknB ) inhibitor with an IC 50 of 12.1 μM. (medchemexpress.com)
MS5033 is a potent PROTAC-based AKT ( protein kinase B) degrader, with a DC 50 of 430 nM in PC3 cells. (medchemexpress.com)
Cdc7-IN-5 (compound I- B ) is a potent Cdc7 kinase inhibitor extracted from patent WO2019165473A1, compound I- B . Cdc7 is a serine-threonine protein kinase enzyme which is essential for the initiation of DNA replication in the cell cycle. (medchemexpress.com)
B -Raf IN 5 (compound 3 b ) is a potent inhibitor of protein kinase B-Raf with an IC 50 of 2.0 nM. (medchemexpress.com)
OTS964 hydrochloride is an orally active, high affinity and selective TOPK (T-lymphokine-activated killer cell-originated protein kinase ) inhibitor with an IC 50 of 28 nM. (medchemexpress.com)
ROCK-IN-5 (compound I- B -37) is a potent inhibitor of ROCK , ERK , GSK , and AGC protein kinases . (medchemexpress.com)
In Drosophila germline progenitor cells, recruitment of the P-TEFb kinase complex to promoter regions is directly inhibited by a small protein encoded by the polar granule component ( pgc ) gene. (biomedcentral.com)

Here we use a battery of spectroscopic methods that track different catalytic elements of the kinase domain to show that the ~100 fold activation of the mitotic kinase Aurora A (AurA) by phosphorylation occurs without a population shift from the DFG-Out to the DFG-In state, and that the activation loop of the activated kinase remains highly dynamic. (elifesciences.org)
For many years it had been thought that the purpose of activation loop phosphorylation was to clamp the otherwise flexible activation loop in an active state that allows molecules that need to be phosphorylated to bind to the kinase. (elifesciences.org)
3. Aurora A site specific TACC3 phosphorylation regulates astral microtubule assembly by stabilizing γ-tubulin ring complex. (nih.gov)
10. NDEL1 phosphorylation by Aurora-A kinase is essential for centrosomal maturation, separation, and TACC3 recruitment. (nih.gov)
12. TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. (nih.gov)
Therefore Tripolin A reveals a new way of regulating mitotic MT stabilizers through Aurora A phosphorylation. (forumbcn2004.org)
Cyclin-dependent kinases are regulated by phosphorylation and dephosphorylation events. (nih.gov)
Anemarsaponin B also inhibits the phosphorylation of MAP kinase kinases 3/6 (MKK3/6) and mixed lineage kinase 3 (MLK3). (medchemexpress.com)

Aurora A is a member of a family of mitotic serine/threonine kinases. (wikipedia.org)
The aurora kinase family of serine/threonine kinases consists of three members, designated aurora kinases A, B, and C. [ 4 ] Aurora kinases A and B are expressed in many different cell types, whereas the expression of aurora kinase C is restricted to testicular tissue. (medscape.com)
Aurora kinase A (Aurora-A), a serine / threonine kinase , plays a pivotal role in various cellular processes, including mitotic entry, centrosome maturation and spindle formation. (bvsalud.org)

The Aurora kinase A is associated with centrosome maturation and separation and thereby regulates spindle assembly and stability. (wikipedia.org)

located on the activation loop of the kinase. (biomedcentral.com)

OTS964 hydrochloride is also a potent inhibitor of the cyclin-dependent kinase CDK11 , which binds to CDK11 B with a K d of 40 nM. (medchemexpress.com)
Vanicoside B targets cyclin-dependent kinase 8 ( CDK8 ) and exhibits anti-tumor activity. (medchemexpress.com)

The discovery that activated forms of Aurora A can have different dynamic properties raises the possibility that inhibitor molecules could be designed to exploit these differences and block specific activities of Aurora A in cancer cells. (elifesciences.org)
[ 2 ] Pazopanib, a tyrosine kinase inhibitor, was recently approved and although it delays progression in some patients, responses remain rare. (medscape.com)
Tripolin A is predicted to bind Aurora A similarly but not identical to MLN8054, therefore it could be used to dissect pathways orchestrated by Aurora kinases as well as a scaffold for further inhibitor development. (forumbcn2004.org)
In this study, we found that phthalazinone pyrazole (PP), a selective inhibitor of Aurora-A kinase (Aurora-A), suppressed the EMT during the differentiation of hepatocyte-like cells (HLCs) from human embryonic stem cells. (korea.ac.kr)

Additionally, Pericentrin recruits enzymes that are involved in centrosome duplication and maturation, including the critical centrosome regulating enzyme, Aurora-A kinase. (wwu.edu)
includes extended chromosomes with defective condensation.9 Top2 depletion also induces mitotic catastrophe failure of cell division and cell death.8 Aurora kinase is another key enzyme during Caudatin mitosis. (monossabios.com)

Mitotic kinases are the main proteins that coordinate accurate mitotic processing [ 1 ]. (biomedcentral.com)
Among the network of regulatory proteins, Aurora kinases are of particular importance. (forumbcn2004.org)

Tripolin A reduced the localization of pAurora A on PJS spindle microtubules (MTs), affected centrosome integrity, spindle formation and length, as well as MT dynamics in interphase, consistent with Aurora A inhibition by RNAi or other specific inhibitors, such as MLN8054 or MLN8237. (forumbcn2004.org)
Moreover, we found that these effects were mediated though suppression of the AKT pathway, which is involved in induction of the EMT, and upregulation of hepatocyte nuclear factor 4α expression rather than Aurora-A inhibition. (korea.ac.kr)
In conclusion, these findings provided insights into the regulatory role of the EMT on in vitro hepatic maturation, suggesting that inhibition of the EMT may drive transformation of hepatoblast cells into mature and polarized HLCs. (korea.ac.kr)
Inhibition of Top2 or Aurora A partially blocked LSA-induced PCC. (monossabios.com)
Inhibition of Mer and Axl receptor tyrosine kinases in astrocytoma cells leads to increased apoptosis and improved chemosensitivity. (ucdenver.edu)
Inhibition of Aurora Kinase A enhances chemosensitivity of medulloblastoma cell lines. (ucdenver.edu)

During oocyte maturation RSK both phosphorylates and down-regulates Myt1 causing prophase I arrest (24). (biospraysehatalami.com)
Lee S, Jo Y, Namgoong S, Kim N. Anillin controls cleavage furrow formation in the course of asymmetric division during mouse oocyte maturation. (labome.cn)

Aurora kinase C (AURKC) is a component of the chromosome passenger complex and is highly expressed in gametes. (nih.gov)
Are single nucleotide variants (SNVs) in Aurora kinases B and C (AURKB, AURKC) associated with risk of aneuploid conception? (nih.gov)

During the last two decades, several studies have shown amplification and overexpression of Aurora kinase A (AURKA) in several GI malignancies. (biomedcentral.com)
Overall, this work provides a foundation for future experiments to determine effects of Pericentrin overexpression on Aurora-A kinase, a model of regulation of centriolar satellites via the Golgi, as well as a novel method for characterizing autofluorescence using FLIM. (wwu.edu)
Overexpression or gene -amplification/ mutation of Aurora-A kinase occurs in different types of cancer , including lung cancer , colorectal cancer , and breast cancer . (bvsalud.org)

Aurora A regulates several important steps in cell division, and plays important roles in several kinds of cancer. (elifesciences.org)
14. AIBp regulates mitotic entry and mitotic spindle assembly by controlling activation of both Aurora-A and Plk1. (nih.gov)
Although Pericentrin recruits Aurora-A, it is not clear whether Pericentrin regulates Aurora-A kinase activity. (wwu.edu)

In terms of enzymatic activity, Aurora kinases belong to the Ser/Thr kinase family and they comprise of two domains: a regulatory domain at the NH2-terminus and a catalytic domain at the COOH-terminus. (forumbcn2004.org)

Aurora kinase A (AURKA) is commonly overexpressed in sarcoma. (medscape.com)
Aurora kinase A (AURKA) is located at the centrosome and required for centrosome maturation and division as well as the formation of the mitotic spindle. (medscape.com)
Alisertib inhibited aurora kinase A activity in HCT-116 human colon cancer cells with a half-maximal inhibitory concentration (IC 50 ) of 6.7 nM and was ~200-fold more selective for AURKA than aurora kinase B (IC 50 = 1534 nM). (medscape.com)

The Xenopus, Drosophila, and Caenorhabditis elegans genomes, on the other hand, contain orthologues only to Aurora A and Aurora B. In all studied species, the three Aurora mitotic kinases localize to the centrosome during different phases of mitosis. (wikipedia.org)
Aurora A and Aurora B kinases play important roles in mitosis. (wikipedia.org)
Finally, Aurora A helps orchestrate an exit from mitosis by contributing to the completion of cytokinesis- the process by which the cytoplasm of the parent cell is split into two daughter cells. (wikipedia.org)
Though the exact mechanism by which Aurora A aids cytokinesis is unknown, it is well documented that it relocalizes to the mid-body immediately before the completion of mitosis. (wikipedia.org)
It phosphorylates condensins and is essential for their chromosome association and functions.10-12 Aurora kinase also promotes chromosome condensation by phosphorylating histone H3 at serines 10 and 28.12 Hence epigenetic modifications are coordinated with chromosome condensation.13 Chromosome condensation outside mitosis is known as premature chromosome condensation (PCC). (monossabios.com)

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SRC is a non-receptor tyrosine kinase that plays critical roles in numerous cellular processes, such as cell proliferation, differentiation, migration, and survival. (cusabio.com)

Auroras share a great degree of homology in their catalytic domain, whereas differ in their NH2-terminal domain. (forumbcn2004.org)

γ-tubulins, the base structure from which centrosomal microtubules polymerize, are also recruited by Aurora A. Without Aurora A the centrosome does not accumulate the quantity of γ-tubulin that normal centrosomes recruit prior to entering anaphase. (wikipedia.org)
1. The centrosomal adaptor TACC3 and the microtubule polymerase chTOG interact via defined C-terminal subdomains in an Aurora-A kinase-independent manner. (nih.gov)

To realize this goal we need to better understand how a kinase switching between active and inactive states affects the ability of inhibitors to interact with it. (elifesciences.org)
Noticeably, our review also summarizes the promising synthetic lethality strategy for Aurora-A inhibitors in RB1, ARID1A and MYC gene mutation tumors , and potential synergistic strategy for mTOR, PAK1, MDM2, MEK inhibitors or PD-L1 antibodies combined with targeting Aurora-A kinase . (bvsalud.org)
In addition, we discuss the design and development of the novel class of Aurora-A inhibitors in precision medicine for cancer treatment . (bvsalud.org)

As the cell cycle progresses, concentrations of Aurora A increase and the kinase associates with the mitotic poles and the adjacent spindle microtubules. (wikipedia.org)
Right before mitotic exit, Aurora A relocalizes to the mid-zone of the spindle. (wikipedia.org)
Aurora A is critical for proper formation of mitotic spindle. (wikipedia.org)
Furthermore, Aurora A is necessary for the proper separation of the centrosomes after the mitotic spindle has been formed. (wikipedia.org)
Without Aurora A, the mitotic spindle, depending on the organism, will either never separate or will begin to separate only to collapse back onto itself. (wikipedia.org)
5. Aurora-A-Dependent Control of TACC3 Influences the Rate of Mitotic Spindle Assembly. (nih.gov)
Fluorescence intensity (arbitrary units) of HURP bound on spindle MTs was quantified in control and Aurora A depleted metaphase cells (n20 cells for each group, from at least two independent experiments). (forumbcn2004.org)
HURP is a MT stabilizer with distinct features since it localizes mainly to kinetochore MTs (kt-MTs) of the mitotic spindle [9], [10] and induces a unique MT conformation kinase assays was determined. (forumbcn2004.org)

Specifically, the mechanisms of Aurora-A kinase promote acquired resistance through modulating DNA damage repair, feedback activation bypass pathways, resistance to apoptosis , necroptosis and autophagy , metastasis , and stemness. (bvsalud.org)

Open in a separate window Figure 1 Tripolins inhibit Aurora kinase activity kinase assay. (forumbcn2004.org)

Eumycetoma Since causing c Met mutations have already been identified in many cancers, we fully sequenced c achieved exons encoding the juxtamembrane domain through the tyrosine kinase domain. (plapathway.com)

In the merged images Aurora A is pseudocolored red, pericentrin green, DNA blue. (forumbcn2004.org)

The aurora kinases were first identified in 1990 during a cDNA screen of Xenopus eggs. (wikipedia.org)
In the case of the former, it has been suggested that Aurora A cooperates with the kinase Nek2 in Xenopus to dissolve the structure tethering the cell's centrosomes together. (wikipedia.org)

Abstract After cheap motilium 10 mg online ingestion at upbroach at macrophages, Legionella pneumophila inhibits acidification and maturation of its phagosome. (ika.ie)

Emerging roles of Aurora-A kinase in cancer therapy resistance. (bvsalud.org)
This review highlights the most recent advances in the oncogenic roles and related multiple cancer hallmarks of Aurora-A kinase -driving cancer therapy resistance, including chemoresistance ( taxanes , cisplatin , cyclophosphamide ), targeted therapy resistance (osimertinib, imatinib , sorafenib , etc.), endocrine therapy resistance ( tamoxifen , fulvestrant ) and radioresistance. (bvsalud.org)

For example, deletion of Aurora A in C. elegans results in an initial separation of the cell's centrosomes followed by an immediate collapse of the asters. (wikipedia.org)

In December 2019, Aurinia Pharmaceuticals announced positive efficacy and safety results from its pivotal AURORA Phase III trial of voclosporin, in combination with mycophenolate (MMF) and low-dose corticosteroids, for lupus nephritis treatment . (delveinsight.com)

The human genome contains three members of the aurora kinase family: Aurora kinase A, Aurora kinase B and Aurora C kinase. (wikipedia.org)

15. Transforming acidic coiled-coil 3 and Aurora-A interact in human thyrocytes and their expression is deregulated in thyroid cancer tissues. (nih.gov)
Alteration of Aurora-A impacts multiple cancer hallmarks, especially, immortalization, energy metabolism , immune escape and cell death resistance which are involved in cancer progression and resistance. (bvsalud.org)

Aurora A is activated by one or more phosphorylations and its activity peaks during the G2 phase to M phase transition in the cell cycle. (wikipedia.org)
Intriguingly, abolishment of Aurora A through RNAi interference results in different mutant phenotypes in different organisms and cell types. (wikipedia.org)
Cell department is controlled through cooperation of different kinases. (biospraysehatalami.com)
We reviewed CCS derived cell lines and unearthed that cMet was phosphorylated and expressed on tyrosine residues in the kinase domain in two of the three lines throughout normal development. (plapathway.com)
B-cell specific deletion of in BRL-15572 mice results in mislocalization of marginal zone B-cells and defective antigen-induced B-cell maturation [16]. (researchensemble.com)

Therefore, without proper expression of Aurora A, the cell's centrosomes are never able to separate. (wikipedia.org)
Pilomyxoid Astrocytoma (PMA) Shows Significant Differences in Gene Expression vs. Pilocytic Astrocytoma (PA) and Variable Tendency Toward Maturation to PA. (ucdenver.edu)

Kitagawa M, Fung S, Onishi N, Saya H, Lee S. Targeting Aurora B to the equatorial cortex by MKlp2 is required for cytokinesis. (labome.cn)

Siegert P, Schmidt G, Papatheodorou P, Wieland T, Aktories K, Orth J. Pasteurella multocida toxin prevents osteoblast differentiation by transactivation of the MAP-kinase cascade via the G? (labome.cn)

In addition PCC is coupled with topoisomerase II (Top2) and Aurora A hyperphosphorylation and activation. (monossabios.com)

Within this trial, HLA course II alleles modulated the grade of vaccine-induced antibodies and acquired a direct effect on protective efficiency, recommending that HLA-II limited Compact disc4+ T cells inspired protective vaccine replies through the control of antibody maturation (5). (nipponkaigi-tokyo.com)

Anatomy15

Organisms6

Diseases6

Chemicals and Drugs93

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes54

Disciplines and Occupations1

Information Science3

Protein34

Phosphorylation8

Serine3

Centrosome maturation and separation1

Activation loop of the kinase1

Cyclin-depend2

Inhibitor4

Enzyme2

Proteins2

Inhibition6

Oocyte maturation2

AURKC2

Overexpression3

Regulates3

Enzymatic activity1

AURKA3

Mitosis5

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Catalytic domain1

Centrosomal2

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Apoptosis1

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Pericentrin1

Xenopus2

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