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Aurora KinasesAurora Kinase AAurora Kinase BAurora Kinase CProtein-Serine-Threonine KinasesProtein Kinase InhibitorsMitosisProtein KinasesPhosphatidylinositol 3-KinasesMAP Kinase Signaling SystemSpindle ApparatusPhosphorylationChromosome SegregationCalcium-Calmodulin-Dependent Protein KinasesCell Line, TumorPiperazinesKinetochoressrc-Family KinasesAzepinesOrganophosphatesCell Cycle ProteinsBenzamidesM Phase Cell Cycle CheckpointsProtein Kinase CCentrosomeCyclic AMP-Dependent Protein Kinasesp38 Mitogen-Activated Protein KinasesCytokinesisPolyploidyApoptosisHeLa CellsMicrotubule-Associated ProteinsCell CycleMitogen-Activated Protein Kinase 1Cell ProliferationMutationAneugensChondromaOocytesPyrimidinesHistonesCDC2 Protein KinaseMolecular Sequence Datap21-Activated KinasesMitogen-Activated Protein Kinase KinasesJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein Kinase 3Antineoplastic AgentsMicrotubulesMeiosisProtein-Tyrosine KinasesCell Cycle CheckpointsSignal TransductionPyrazolesAnaphaseAmino Acid SequenceChromosomal Proteins, Non-HistoneCollagen Type XICyclin-Dependent KinasesEnzyme InhibitorsBlotting, WesternProtein BindingBlastodiscCreatine KinaseQuinazolinesRNA, Small InterferingMAP Kinase Kinase KinasesPotoroidaeCasein Kinase IIeIF-2 KinaseCasein KinasesRibosomal Protein S6 KinasesCell Physiological ProcessesPyruvate KinaseCell LineEnzyme ActivationMAP Kinase Kinase 1Receptor Protein-Tyrosine KinasesRNA InterferenceCell DifferentiationNuclear ProteinsCentromereGene Expression Regulation, EnzymologicCells, CulturedRNA, MessengerPloidiesMicrotubule-Organizing CenterExtracellular Signal-Regulated MAP KinasesThymidine KinaseCell DivisionMAP Kinase Kinase 4Proto-Oncogene ProteinsSperm MaturationModels, BiologicalMitogen-Activated Protein KinasesNeoplasmsPhosphotransferases (Alcohol Group Acceptor)TransfectionIsoenzymesGlycogen Synthase Kinase 3
CentrosomePhosphorylationProtein kinaseInhibitorMitosisRegulatesMitoticMammalianEffectorSeparationPathwaysDasatinibUpstreamAffinityOncogeneDrosophilaPhosphatidylinositolProteinsPhosphorylatesChromosomal passenger proteinDexamethasoneCentrosomesChromosome segregationMiceAlignmentDephosphorylationInhibitionActivateFamilyOrganismsMicrotubuleAntibodyAssayCell cycleIntegralMembersRole
Centrosome15
  • NDEL1 preferentially localizes to the centrosome and is a likely target for cell cycle-activated kinases, including CDK1. (elsevierpure.com)
  • The Xenopus, Drosophila, and Caenorhabditis elegans genomes, on the other hand, contain orthologues only to Aurora A and Aurora B. In all studied species, the three Aurora mitotic kinases localize to the centrosome during different phases of mitosis. (wikipedia.org)
  • The Aurora kinase A is associated with centrosome maturation and separation and thereby regulates spindle assembly and stability. (wikipedia.org)
  • Aurora A localizes next to the centrosome late in the G1 phase and early in the S phase. (wikipedia.org)
  • γ-tubulins, the base structure from which centrosomal microtubules polymerize, are also recruited by Aurora A. Without Aurora A the centrosome does not accumulate the quantity of γ-tubulin that normal centrosomes recruit prior to entering anaphase. (wikipedia.org)
  • Aurora A influences centrosome maturation and spindle assembly, while Auroras B and C direct chromosome alignment and segregation. (rndsystems.com)
  • An aurora kinase that localizes to the CENTROSOME during MITOSIS and is involved in centrosome regulation and formation of the MITOTIC SPINDLE. (lookformedical.com)
  • On the role of aurora-A in centrosome function. (lookformedical.com)
  • Works in various organisms have revealed that the kinase is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability. (lookformedical.com)
  • Ectopic kinase overexpression in any culture cell line leads to polyploidy and centrosome amplification. (lookformedical.com)
  • HSP90AA1 Aurora kinases are grasp regulators of both centrosome and checkpoint control function. (ap26113.com)
  • GSK1904529A Aurora A is found at centrosomes in early mitosis and controls centrosome maturation centriole separation and chromosome alignment (33) whereas accurate targeting of Aurora B to the chromosomes and then to the spindle midzone and midbody is essential for maintaining microtubule/kinetochore attachments and therefore faithful chromosomal segregation (34). (ap26113.com)
  • Vertii A, Ivshina M, Zimmerman W, Hehnly H , Kant S, Doxsey S. The Centrosome Undergoes Plk1-Independent Interphase Maturation during Inflammation and Mediates Cytokine Release. (syracuse.edu)
  • Aurora A is involved with centrosome readiness, spindle assembly, and uneven protein localization throughout mitosis. (others-signal.com)
  • More over, TPX2 is only necessary for a part of Aurora A dependent processes: TPX2 inactivation by RNAi causes spindle problems and reduction of Aurora A from the mitotic spindle, but centrosome maturation is normal, and the centrosome pool of the kinase is unchanged. (others-signal.com)
Phosphorylation8
  • The expression of Aurora-A phosphorylation-mimetic mutants of NDEL1 efficiently rescued the defects of centrosomal maturation and separation which are characteristic of Aurora-A-depleted cells. (elsevierpure.com)
  • Our findings suggest that Aurora-A-mediated phosphorylation of NDEL1 is essential for centrosomal separation and centrosomal maturation and for mitotic entry. (elsevierpure.com)
  • Treatment of HeLa cells with paclitaxel induces phosphorylation of Aurora A at Thr288 while treatment with hydroxyurea ablates phosphorylation. (cellsignal.com)
  • GR phosphorylation by an Aurora kinase-dependent pathway provides the mechanism explaining the tight coupling GSK1904529A of ligand-independent GR phosphorylation to cell cycle phase because the maximal activity of these kinases occurs in mitosis. (ap26113.com)
  • You can find six phosphorylation sites in RSK1 that are crucial for its activation and its own subsequent part in substrate phosphorylation (21 22 Of the phosphorylation of Ser-380 can be very important to RSK1 activation traveling functions such as for example rules of gene manifestation and protein synthesis and cell cycle regulation as a downstream Nisoxetine hydrochloride kinase in the Mos-MAPK pathway (23). (biospraysehatalami.com)
  • These kinases lead to β-catenin stabilization through phosphorylation-dependent inactivation of GSK-3β either directly or indirectly. (lu.se)
  • In addition, PIP2 binds to polo-like kinase (PLK1) and reduces PLK1-mediated phosphorylation of MCAK. (biomedcentral.com)
  • Cyclin-dependent kinases are regulated by phosphorylation and dephosphorylation events. (bvsalud.org)
Protein kinase5
  • Aurora kinase A also known as serine/threonine-protein kinase 6 is an enzyme that in humans is encoded by the AURKA gene. (wikipedia.org)
  • An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. (lookformedical.com)
  • The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters. (lookformedical.com)
  • This conversion is promoted by the double inhibition (i.e., 2i) of mitogen-activated protein kinase kinase (Map2k), antagonizing Fgf signaling, and of glycogen synthase kinase 3 (Gsk3), stimulating the Wnt pathway. (bioone.org)
  • Conversely, during starvation mTORC1 is inhibited and autophagy is induced by AMP-activated protein kinase (AMPK), leading to generation of intracellular nutrients and energy during degradation of non-functional or non-essential organelles or protein aggregates [ 4 , 6 ], in turn contributing to cell survival. (springer.com)
Inhibitor1
  • The expression of BCL2 varies in T-ALL depending on its stage of maturation, thereby T-ALL displays a heterogenous response to the BCL2-specific inhibitor venetoclax. (lu.se)
Mitosis8
  • Aurora A and Aurora B kinases play important roles in mitosis. (wikipedia.org)
  • Finally, Aurora A helps orchestrate an exit from mitosis by contributing to the completion of cytokinesis- the process by which the cytoplasm of the parent cell is split into two daughter cells. (wikipedia.org)
  • Though the exact mechanism by which Aurora A aids cytokinesis is unknown, it is well documented that it relocalizes to the mid-body immediately before the completion of mitosis. (wikipedia.org)
  • Aurora kinase C is a chromosomal passenger protein that interacts with aurora kinase B in the regulation of MITOSIS. (lookformedical.com)
  • A family of highly conserved serine-threonine kinases that are involved in the regulation of MITOSIS. (lookformedical.com)
  • An aurora kinase that is a component of the chromosomal passenger protein complex and is involved in the regulation of MITOSIS. (lookformedical.com)
  • Mitosis is usually a dynamic process controlled by the activity of multiple kinases with attendant modification of proteins facilitating rigid checkpoint control. (ap26113.com)
  • Contrary to cells treated with a siRNA, cells treated with siRNAs against Bora often displayed multipolar spindles in mitosis, a phenotype that is also seen upon TPX2 RNAi Geneticin distributor and after injection of antibodies blocking Aurora A function. (others-signal.com)
Regulates3
Mitotic10
  • Here, we show that Aurora-A phosphorylates NDEL1 at Ser251 at the beginning of mitotic entry. (elsevierpure.com)
  • Aurora A is a member of a family of mitotic serine/threonine kinases. (wikipedia.org)
  • The kinase discovered, Eg2, is now referred to as Aurora A. However, Aurora A's meiotic and mitotic significance was not recognized until 1998. (wikipedia.org)
  • As the cell cycle progresses, concentrations of Aurora A increase and the kinase associates with the mitotic poles and the adjacent spindle microtubules. (wikipedia.org)
  • Right before mitotic exit, Aurora A relocalizes to the mid-zone of the spindle. (wikipedia.org)
  • Aurora A is critical for proper formation of mitotic spindle. (wikipedia.org)
  • Furthermore, Aurora A is necessary for the proper separation of the centrosomes after the mitotic spindle has been formed. (wikipedia.org)
  • Without Aurora A, the mitotic spindle, depending on the organism, will either never separate or will begin to separate only to collapse back onto itself. (wikipedia.org)
  • Mammalian aurora-A belongs to a multigenic family of mitotic serine/threonine kinases comprising two other members: aurora-B and aurora-C. In this review we will focus on aurora-A that starts to localize to centrosomes only in S phase as soon as centrioles have been duplicated, the protein is then degraded in early G1. (lookformedical.com)
  • A family of serine/threonine kinase Aurora constitutes a key regulator in the orchestration of mitotic events. (lookformedical.com)
Mammalian1
Effector2
  • We observed that dexamethasone exposure caused upregulation of Aurora kinase and its various downstream effector kinases such as JAK, p38, mTOR, and S6K. (lu.se)
  • p21-activated kinase 4 (PAK4), a specific effector of the Rho GTPase Cdc42, is activated by HGF, and we have previously shown that activated PAK4 induces a loss of both actin stress fibres and focal adhesions. (silverchair.com)
Separation1
  • For example, deletion of Aurora A in C. elegans results in an initial separation of the cell's centrosomes followed by an immediate collapse of the asters. (wikipedia.org)
Pathways2
  • 2016 ) Icaritin suppresses development of neuroendocrine differentiation of prostate cancer through inhibition of IL-6/STAT3 and Aurora kinase A pathways in TRAMP mice. (neurotree.org)
  • After transfection, we treated transfected cells with 1µM of CNO or VEH (a control) for 5-30min and then lysed the cells with RIPA Buffer, and prepared samples for western blotting to detect activation of proteins of interest in the GPCR non-canonical signaling pathways (example: kinases and transcription factors). (uw.edu)
Dasatinib2
  • Some kinases are essential scientific objectives of imatinib, nilotinib, and/or dasatinib, although only dasatinib has been reported to inhibit all SRC family kinases. (others-signal.com)
  • A comprehensive kinase interaction map for dasatinib was recently described, though assay differences preclude direct comparison of the kinase users of AP24534 and dasatinib. (others-signal.com)
Upstream1
  • The zinc finger domain of Tzfp binds to the tbs motif located at the upstream flanking region of the Aie1 (aurora-C) kinase gene. (lookformedical.com)
Affinity1
  • Supplementary MaterialsS1 Fig: Amino acidity sequences of M-CSFWT, M-CSFRGD libraries, M-CSFc-FMS, M-CSFv3 as well as the 3 M-CSFRGD variants which were chosen following an affinity maturation process. (biomasswars.com)
Oncogene1
  • Those transformed cells form tumours when implanted in immunodeficient mice, indicating that the kinase is an oncogene. (lookformedical.com)
Drosophila4
  • Taken together, our findings suggest that Bora is just a important activator of Aurora A that is functionally conserved between Drosophila and vertebrates. (others-signal.com)
  • We show here that the conserved binding partner Bora is essential for Aurora A to perform these functions in Drosophila. (others-signal.com)
  • Although in Drosophila, Bora so far may be the only known activator of Aurora A, many in vitro activators of Aurora A have now been discovered in other creatures. (others-signal.com)
  • Therefore, unlike Bora, TPX2 appears to be specifically in charge of the spindle assembly purpose of Aurora A. To date, no TPX2 homolog has been discovered in Drosophila. (others-signal.com)
Phosphatidylinositol1
  • A previous screen of a human kinase and phosphatase shRNA library to select genes that mediate arsenite induction of spindle abnormalities resulted in the identification of phosphatidylinositol-5-phosphate 4-kinase type-2 gamma (PIP4KIIγ), a phosphatidylinositol 4,5-bisphosphate (PIP2)-synthesizing enzyme. (biomedcentral.com)
Proteins2
  • All of these members contain a conserved C-terminal amino acid sequence termed the polo box domain which provides a docking site for certain proteins (1) and a kinase domain which is predicted to activate many protein kinases including Aurora A/B PKA ERK1/2 RSK1/2 Akt/PKB and MEK1 (2). (biospraysehatalami.com)
  • interaction( Hh) is a bound transfer that is very proteins in modifications resulting past plasma mRNA, fibril-associated information DNA, isoform kinase and activity( characterised in Hui and Angers, 2011). (evakoch.com)
Phosphorylates1
  • Active mTORC1 phosphorylates the eukaryotic initiation factor 4E-binding protein-1 (4E-BP1) and 40S ribosomal protein S6 kinase 1 (S6K1) to promote protein synthesis [ 3 , 4 ]. (springer.com)
Chromosomal passenger protein1
  • Although there is evidence to suggest that Aurora C might be a chromosomal passenger protein, the cellular function of it is less clear. (wikipedia.org)
Dexamethasone2
  • On day 7 after maturation induction by insulin, dexamethasone, and 3 isobutyl 1 methylxanthine, cells were challenged with each alternative AIM protein for two days, and the different results of lipolysis were examined. (aurorakinaseb.com)
  • Indeed, we observed partial restoration of dexamethasone sensitivity with a combination of dexamethasone and inhibitors targeting either these kinases or β-catenin. (lu.se)
Centrosomes2
  • In the case of the former, it has been suggested that Aurora A cooperates with the kinase Nek2 in Xenopus to dissolve the structure tethering the cell's centrosomes together. (wikipedia.org)
  • Therefore, without proper expression of Aurora A, the cell's centrosomes are never able to separate. (wikipedia.org)
Chromosome segregation1
Mice1
  • To determine if neovascularization and vessel maturation in the lower limb are improved in Trx2-TG mice we Fostamatinib disodium performed immunohistochemistry Fostamatinib disodium with EC- and pericyte-specific markers. (biomasswars.com)
Alignment1
Dephosphorylation1
  • In vertebrates, TPX2 prevents PP1 dependent dephosphorylation and therefore AZD5363 locks the kinase in its active conformation. (others-signal.com)
Inhibition1
  • Knockdown or inhibition of Src induces multipolar spindle formation, resulting in abnormal expression of the Aurora B and INCENP components of the chromosomal passenger complex. (bvsalud.org)
Activate1
Family2
  • The human genome contains three members of the aurora kinase family: Aurora kinase A, Aurora kinase B and Aurora C kinase. (wikipedia.org)
  • This feature probably contributes to the relatively wide kinase specificity profile of AP24534, including VEGFR and FGFR family kinases, receptors perhaps not restricted by the three currently authorized BCR ABL drugs. (others-signal.com)
Organisms1
  • Intriguingly, abolishment of Aurora A through RNAi interference results in different mutant phenotypes in different organisms and cell types. (wikipedia.org)
Microtubule2
  • In the absence of Aurora A mad2, a protein that normally dissipates once a proper kinetochore-microtubule connection is made, remains present even into metaphase. (wikipedia.org)
  • TPX2/ Aurora A binding is stimulated by the little GTPase Ran, which in turn is activated by RCC1, an exchange factor that is found on condensed chromatin and is involved with microtubule nucleation and spindle formation. (others-signal.com)
Antibody2
  • The corresponding western blots using phospho-Aurora A (Thr288) antibody (left panel) and β-Actin antibody (right panel) are shown in the lower figure. (cellsignal.com)
  • Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-Aurora A (Thr288) in a single step. (cellsignal.com)
Assay1
  • The rapid protocol (RP) PathScan ® RP Phospho-Aurora A (Thr288) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Aurora A protein phosphorylated at Thr288 in a reduced assay time of 1.5 hours. (cellsignal.com)
Cell cycle2
  • Aurora A is activated by one or more phosphorylations and its activity peaks during the G2 phase to M phase transition in the cell cycle. (wikipedia.org)
  • Protein kinases that control cell cycle progression in all eukaryotes and require physical association with CYCLINS to achieve full enzymatic activity. (bvsalud.org)
Integral1
  • The DPY19L2 protein is involved in the development of the acrosome and elongation of the sperm head, which are integral steps in sperm cell maturation. (medlineplus.gov)
Members1
  • Because the members of the PIP5KI and PIP4KII families exhibit specific and distinct subcellular localization patterns, it has been postulated that local increases in PIP2 concentration by a specific kinase member may underlie its diverse signaling functions [ 5 , 8 ]. (biomedcentral.com)
Role1