• The expression and regulation of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) in neuroectodermal precursor cells is undocumented. (uea.ac.uk)
  • Matrix metalloproteinases (MMPs) are a large family of mostly secreted, extracellularly acting proteolytic enzymes. (nature.com)
  • Matrix metalloproteinases (MMPs) are extracellularly acting enzymes that have long been known to have deleterious roles in brain injury and disease. (nature.com)
  • The balance of metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) is crucial for the manifestation and progression of periodontal diseases and is one of the promising areas of scientific research in terms of developing methods for selective inhibition of MMPs. (spr-journal.ru)
  • Correlation analysis revealed an inverse relationship of TIMPs expression parameters with those MMPs, including those most significant for the development of AgP - MMP1 (ρ = -0.40), MMP8 (ρ = -0.34), and MMP14 (ρ = -0.24). (spr-journal.ru)
  • Our results show an increase in the expression of TIMP1 and TIMP2 in various forms of periodontitis and an inverse relationship with the MMPs inhibited by them. (spr-journal.ru)
  • Metalloprotease inhibitors are cellular inhibitors of the Matrix metalloproteinases (MMPs). (wikipedia.org)
  • The expression of MMPs is increased in various pathological conditions like inflammatory conditions, metabolic bone disease, to cancer invasion, metastasis and angiogenesis. (wikipedia.org)
  • Due to the role of MMPs in pathological conditions, inhibitors of MMPs may have therapeutic potential. (wikipedia.org)
  • Most MMP inhibitors are unable to target specific MMPs connected to specific pathological conditions. (wikipedia.org)
  • α2-Macroglobulin (α2M) is a protease inhibitor which inhibits activated MMPs. (wikipedia.org)
  • MMPs are associated with the cell surface or bound to the extracellular matrix which prevents them from diffusing away and keeps the MMP under control of the cell. (wikipedia.org)
  • The structural difference MMPs' is mainly in the S1' side and by modifying the P1' group, inhibitor selectivity can be developed. (wikipedia.org)
  • The underlying mechanisms of matrix destruction in TB remain poorly understood but consideration of the lung extracellular matrix predicts that matrix metalloproteinases (MMPs) will play a central role, owing to their unique ability to degrade fibrillar collagens and other matrix components. (ersjournals.com)
  • Since we proposed the concept of a matrix degrading phenotype in TB a decade ago, diverse data implicating MMPs as key mediators in TB pathology have accumulated. (ersjournals.com)
  • Remodeling of atrial extracellular matrix (ECM) in atrial fibrillation (AF) involves changes in the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs). (unboundmedicine.com)
  • Nerve growth factor (NGF) is generated from proNGF, which is either cleaved in the Golgi network or secreted and cleaved extracellularly by matrix metalloproteinases (MMPs). (jneurosci.org)
  • Matrix metalloproteinases (MMPs) are a family of endopeptidases playing a key role in tissue remodelling in both physiological and pathological conditions. (nih.gov)
  • This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). (cancerindex.org)
  • Matrix metalloproteinases (MMPs) have been considered to be associated with tumor progression or poor prognosis. (cancerindex.org)
  • BACKGROUND AND PURPOSE: Elevated levels of matrix metalloproteinases (MMPs), particularly MMP-1 and MMP-9, have been implicated in plaque rupture. (edu.au)
  • The concentrations of MMPs and doxycycline were determined in the atherosclerotic tissue by enzyme-linked immunosorbent assay and high-performance liquid chromatography, respectively. (edu.au)
  • The activities of matrix metalloproteinase (MMP)-2 and−9 were further inhibited and TIMP-1 and−2, and NM23-H1, the MMPs negative modulators, were further activated in the combined treatment. (frontiersin.org)
  • The metastasizing cells will proliferate in large numbers in situ and secrete proteolytic enzymes, such as the matrix metalloproteinases (MMPs), which degrade the extracellular matrix and blood vessel wall. (frontiersin.org)
  • Development of most solid tumors is characterized by an increase in secretion and activation of matrix metalloproteinases (MMPs) produced by either the tumor cells or tumor-associated fibroblasts. (bvsalud.org)
  • One of the main difficulties in studying the mechanism of action of these matrix metalloproteinases (MMPs) derives from the physicochemical properties of the natural triple-helical collagen, which makes ii difficult to handle. (uni-bielefeld.de)
  • The first generation of MMP inhibitors were based on the structure of the collagen molecule. (wikipedia.org)
  • In the design of these inhibitors, the basic protein backbone of collagen is maintained but the amide bond is replaced with a zinc-binding group. (wikipedia.org)
  • Collagen VI (COL6) is highly expressed in adipose tissue, upregulated in the obese state, and enriched in breast cancer lesions and is a stimulator of mammary tumor growth. (jci.org)
  • Conclusions: The results confirm the usefulness of synthetic heterotrimeric collagenous peptides in the folded and unfolded state as mimics of the natural substrates collagen and gelatin, respectively, to gain a better a insight into the proteolytic mechanisms of matrix metalloproteinases. (uni-bielefeld.de)
  • Recognition and catabolism of synthetic heterotrimeric collagen peptides by matrix metalloproteinases", CHEMISTRY & BIOLOGY , vol. 7, 2000, pp. 119-132. (uni-bielefeld.de)
  • Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. (cancerindex.org)
  • After ingestion of melanoma-derived exosomes during incubation, mouse embryonic fibroblasts cells emerged cellular invasiveness with the presentation of a marked increase in miR-21 expression. (dovepress.com)
  • In the present study, polymerase chain reaction and western blot analyses were performed to examine the expression levels of miR‑130a and associated proteins. (spandidos-publications.com)
  • 10 High expression of miR-21 in melanoma cells inhibits mRNA expression of crucial tumor suppressor proteins such as the tissue inhibitor of metalloproteinases 3 (TIMP3). (dovepress.com)
  • These proteins play a master role in diverse physiological processes and diseases such as the homeostatic tissue remodeling and cancer 17 . (bvsalud.org)
  • LTP-associated MMP proteolysis is probably then terminated by an increase in the activity of endogenous inhibitors called tissue inhibitors of metalloproteinases. (nature.com)
  • MMP inhibitors can broadly be subdivided into non-synthetic (e.g. endogenous) or synthetic. (wikipedia.org)
  • This group of inhibitors contain a hydroxamate (-CONHOH) group that binds the zinc atom in the active site of the MMP enzyme. (wikipedia.org)
  • The inhibitor binds to the zinc at the active center of the enzyme, thereby blocking its activity. (wikipedia.org)
  • Le and Friedman present evidence that seizures initiate this process by inducing expression of the tissue inhibitor of matrix metalloproteinase 1 (TIMP-1), which binds to proMMP-7, thus preventing formation of mature MMP-7. (jneurosci.org)
  • Classical lock and key inhibitors such as phosphoramidon and bestatin bind tightly by approximating the transition state of the hydrolysis of the peptide, preventing it from acting on other substrates. (wikipedia.org)
  • During abruptions, decidual cell and neutrophil-derived proteases effectively degrade the decidual and fetal membrane extracellular matrix to promote preterm premature rupture of the membranes and preterm delivery (PTD). (scirp.org)
  • Tissue inhibitor of metalloproteinase 2 (TIMP2), an antagonist of matrix metalloproteinase 2 (MMP2), was identified as a novel, direct target gene of miR‑130a. (spandidos-publications.com)
  • The invasion analysis showed that exosome-treated fibroblast cells had greater invasive capability as compared to untreated fibroblast cells, with the higher expressions of MMP2 and MMP9. (dovepress.com)
  • These enzymes have the ability to break down connective tissue. (wikipedia.org)
  • Dental pulp is loose connective tissue in the pulp cavity surrounded by rigid dentin and is necessary for tooth nutrition, innervation, and immunocompetency[1]. (researchsquare.com)
  • Key risk factors for a steroid response include high myopia, type 1 diabetes mellitus, connective tissue disorders (e.g., rheumatoid arthritis), pigment dispersion, traumatic angle recession, 2 primary open-angle glaucoma, prior penetrating keratoplasty, the duration of steroid therapy and the steroid's anti-inflammatory potency. (reviewofophthalmology.com)
  • In summary, the present study suggests that HPV18 E6 promotes the expression of miR‑130a, which further inhibits the expression of TIMP2 and promotes cervical cancer cell invasion. (spandidos-publications.com)
  • MiR-21 promoted invasion of fibroblasts by down-regulation of tissue inhibitor of metalloproteinase 3 (TIMP3) expression and increasing of matrix metalloprotein (MMP) expression in fibroblast cells via melanoma-derived exosomes in a time-dependent manner. (dovepress.com)
  • These effects were partially mediated through enhanced TGF-β signaling, which contributes to tissue fibrosis and epithelial-mesenchymal transition (EMT) of tumor cells. (jci.org)
  • Gouda and Bhandary, 2018 ), which leads to excessive deposition of extracellular matrix (ECM) and ultimately leads to the formation of fibrotic lesions ( Pardo and Selman, 2002 ). (frontiersin.org)
  • The expression of TIMP-1, TIMP-2 and TIMP-3 was unchanged following stem cells differentiation into neurons and glia. (uea.ac.uk)
  • Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
  • The metastasizing cells penetrate the blood vessel wall by invasiveness, and migrate in the circulation, extravasate out of the blood vessels, colonize in the distant tissue, initiate angiogenesis, and finally grow at the new site ( 2 , 3 ). (frontiersin.org)
  • This complements both the fundamental knowledge of the development and progression of periodontal pathology and may have applied significance in terms of using the studied TIMP1 and TIMP2 expression criteria to establish the aggressive course of periodontitis already at the stage of disease manifestation, which will allow to individualize treatment, prevent or slow down tooth loss in order to preserve and/or improve the quality of life of this patient group. (spr-journal.ru)
  • In situ hybridization (ISH) was used to investigate the presence of HPV-16, while immunohistochemistry (IHC) was used to estimate the expression level of TIMP-2. (bvsalud.org)
  • Deficits in synaptic remodelling are associated with psychiatric and neurological disorders, and aberrant MMP expression or function may contribute to the molecular mechanisms underlying these deficits. (nature.com)
  • Other inhibitor mechanisms are possible. (wikipedia.org)
  • During the metastasis, cancer cells in situ move to other tissues and organs from the blood circulation or the lymphatic system through proliferation, adhesion, invasion, and migration. (frontiersin.org)
  • 11 This results in increased matrix metalloproteinase activity, playing a pivotal role in the process of invasion and metastasis. (dovepress.com)
  • By integrating different datasets from the Gene Expression Omnibus (GEO) database, we analysed a merged expression matrix of pulpitis, aiming to identify biological pathways and diagnostic biomarkers of pulpitis. (researchsquare.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • This study evaluated the in situ activity and expression of gelatinases (MMP-2 and MMP-9) and their relationship with TIMP-1 or TIMP-3 in atria undergoing rapid atrial pacing for the induction of AF (4 weeks' pacing followed by 2 weeks of maintained AF) in pigs. (unboundmedicine.com)
  • One is a chelating moiety that interacts with the zinc ion and the other is a hydrophobic extension from the catalytic site that project into S1' pocket (P1' group) of the metalloproteinase. (wikipedia.org)
  • Lung extracellular matrix destruction is essential to TB transmission and consequently of critical importance to the global success of Mtb. (ersjournals.com)
  • The mechanism whereby Mtb targets the lung apices is unknown, but once it reaches the lung interstititum, breakdown of the extracellular matrix must occur for the pathogen to disseminate via the airways. (ersjournals.com)
  • Cavities are often several centimetres across, so there must be very extensive lung matrix destruction for such cavitation to occur. (ersjournals.com)
  • Lung matrix destruction is critical to the life cycle of Mycobacterium tuberculosis (Mtb). (ersjournals.com)
  • CUR significantly improved the degree of fibrosis, levels of inflammation, and oxidative imbalances in lung tissue in animal models of PF. (frontiersin.org)
  • Various potential MMP inhibitors will be explained in the following sections, including information on their development, structure-activity relationship and pharmacokinetics. (wikipedia.org)
  • TIMP-1 was found to be mostly colocalized with gelatinase activity over the AF tissues, implying the coexistence of gelatinase activity and TIMP-1, but TIMP-3 appeared only partially colocalized and discontinued the gelatinase activity surrounding the cardiomyocytes. (unboundmedicine.com)
  • Since little information is available about their role in celiac disease (CD), our aims were to quantify their expression/activity and to investigate their relation to proinflammatory cytokines in this condition. (nih.gov)
  • Furthermore, reverse transcription‑quantitative PCR was used to examine the expression levels of circular RNA‑PVT1 (circ‑PVT1), micoRNA‑195‑5p (miR‑195‑5p) and ETS1 in GC tissues and cells. (spandidos-publications.com)
  • TIMP-4 showed a 23-fold reduction in media conditioned by differentiated cells compared with stem cell-conditioned media, reflecting a 6-fold decrease in mRNA expression. (uea.ac.uk)
  • Expression of MMP-7 (green) decreases and cell death (red) increases in rat hippocampal slices after kainic acid treatment (right). (jneurosci.org)
  • Furthermore, the expression of miR‑130a was also higher in HPV(+) cervical cancer cell lines compared with that in HPV(‑) cells. (spandidos-publications.com)
  • Abruptions (decidual hemorrhage) produce excess thrombin which acts as autocrine/paracrine inducer of decidual cell expressed matrix metalloproteinases and of neutrophil chemoattractant and activator, interleukin-8. (scirp.org)
  • Epithelial-mesenchymal transition (EMT) is a key cellular process in which immotile epithelial cells transform into mesenchymal cells through cell polarity loss, cell-cell junction disassembly, and extracellular matrix (ECM) alteration. (bmbreports.org)
  • To determine the expression of tissue inhibitors of metalloproteinases (TIMP-2) in oral squamous cell carcinoma (OSCC) and the difference in its expression level between positive and negative HPV-16 (human papilloma virus- 16) OSCC patients. (bvsalud.org)
  • Aims - to determine the significance of TIMP1 and TIMP2 expression parameters in gingival biopsies to identify the type of periodontitis at the stage of disease manifestation. (spr-journal.ru)
  • ROC analysis established acceptable informativeness of all studied parameters of total expression and positivity of epithelial expression of TIMP1, as well as positivity of total expression and intensity of epithelial expression of TIMP2 to distinguish between aggressive and chronic forms of periodontitis at the stage of disease manifestation. (spr-journal.ru)
  • The expression of TIMP2 was negatively mediated by miR‑130a, and HPV18 E6 inhibited the expression of TIMP2 in HeLa cells. (spandidos-publications.com)
  • 2009) Progestin and thrombin regulate tissue factor expression in human term decidual cells. (scirp.org)
  • This gene exhibits elevated expression levels in multiple human cancers. (cancerindex.org)
  • In contrast, while MMP-2 and TIMP-4 were localized to both stem and mature CNS cells, their levels of expression were substantially reduced in the latter. (uea.ac.uk)
  • As a result, MMP-7 levels decline, proNGF levels rise, and 75 NTR expression and caspase activation increase. (jneurosci.org)
  • CONCLUSIONS: Doxycycline penetrated atherosclerotic plaques with acceptable tissue levels. (edu.au)
  • The expression levels of circ‑PVT1 and ETS1 were increased in GC tissues and cells, and miR‑195‑5p was decreased. (spandidos-publications.com)
  • OPUS at UTS: Prospective, randomized, double-blind trial investigating the effect of doxycycline on matrix metalloproteinase expression within atherosclerotic carotid plaques. (edu.au)
  • The aim of the study was to investigate the ability of doxycycline, a nonspecific MMP inhibitor, to reduce MMP concentration in carotid atheroma. (edu.au)
  • Pathological examination with morphometric analysis of TIMPs expression and subsequent statistical data analysis was performed using AperioImageScope v12.4.0.5043, Statistica 10.0, MedCalc19.6. (spr-journal.ru)
  • When the hard dental tissue is broken, various stimuli may induce pathological changes in dental pulp, which is difficult to heal due to the lack of collateral circulation[2]. (researchsquare.com)
  • If harmful stimuli cannot be removed in time, increasing numbers of immune cells are recruited to the pulp tissue[5] and the immune-inflammatory balance is disrupted. (researchsquare.com)
  • De- cidual cells promote hemostasis through enhanced expression of tissue factor (TF), the primary initiator of hemostasis via thrombin generation, and plasminogen activator inhibitor-1, which inactivates tissue type plasminogen activator, the primary fibrinolytic agent. (scirp.org)
  • Two molecular features of most MMP inhibitors are responsible for the affinity. (wikipedia.org)
  • MMP expression varied between different types of IPMN, a result supporting differences in molecular basis of malignancies. (cancerindex.org)
  • MMP high expression groups (MMP score ≥5) had worse prognosis than low-expression groups. (cancerindex.org)
  • Duodenal biopsies from untreated, treated celiac patients and controls were used to quantify the expression of MMP-1, MMP-2, MMP-3, MMP-9, MMP-12, MMP-14, their inhibitor TIMP-1, IFN-gamma and TNF-alpha by using real-time reverse transcription-polymerase chain reaction and the gelatin/casein/elastin activities by gel zymography, and to isolate lamina propria mononuclear cells (LPMCs). (nih.gov)
  • Knockdown of HPV18 E6 significantly inhibited the expression of miR‑130a in HeLa cervical cancer cells. (spandidos-publications.com)
  • Additionally, the treatment with propofol could lead to decreased circ‑PVT1 expression in GC cells. (spandidos-publications.com)
  • Additionally, the effects of transfection with circ‑PVT1 small interfering RNA (si‑circ‑PVT1) on HGC‑27 and AGS cells could be reversed by treatment with miR‑195‑5p inhibitor. (spandidos-publications.com)
  • In GC, Yang et al ( 7 ) reported that propofol suppressed the proliferation of SGC-7901 and MGC-803 cells by promoting inhibitor of growth 3 ( 7 ). (spandidos-publications.com)
  • Peng and Zhang ( 8 ) indicated that propofol inhibited proliferation, but induced apoptosis of SGC-7901 cells by regulating matrix metalloproteinase (MMP)2 ( 8 ). (spandidos-publications.com)
  • Moreover, ETP expression was associated with aggressive mammary tumor growth and high metastatic growth. (jci.org)
  • High expression of miR‑130a was significantly associated with lymph node metastasis and an advanced clinical stage of cervical cancer. (spandidos-publications.com)
  • The percentage of samples that appeared to accommodate detectable HPV-16 was high, but no significant difference was observed in relation to TIMP-2 expression level. (bvsalud.org)
  • The mechanism of primary open-angle glaucoma involves an increase of transforming growth factor beta-2 (TGFB2), which causes dysregulation of the extracellular matrix within the juxtacanalicular trabecular meshwork. (reviewofophthalmology.com)
  • There are, in addition, other cals that are able to provoke an immune response such that characteristics, such as the ability to increase matrix allergic sensitization of the respiratory tract will be metalloproteinase expression or to cause perturbation of acquired. (cdc.gov)
  • 16 However, this mechanism has been observed only in cadaver eyes that were medically treated and/or had previously undergone surgery, not in untreated trabecular meshwork tissue. (reviewofophthalmology.com)
  • Tilley, R. and Mackman, N. (2006) Tissue factor in hemostasis and thrombosis. (scirp.org)
  • A total of 470 DEGs comprising 394 upregulated and 76 downregulated genes were found in pulpitis tissue. (researchsquare.com)
  • There was no difference in any other MMP (MMP-2, -3, or -9) or tissue inhibitor of matrix metalloproteinases-1 or -2. (edu.au)
  • The TIMP-2 was expressed in 27 (81.8%) of OSCC sections with no significant difference between its expression level in HPV-16 positive and HPV-16 negative OSCC cases (p=0.058). (bvsalud.org)
  • CB1 and CB2 have been cloned and characterized from mammalian tissues, the main difference between them being their tissue expression pattern [ 6 ]. (oncotarget.com)
  • Recent studies indicate that aberrant MMP expression, localization and function may contribute to synaptic plasticity deficits associated with such disorders. (nature.com)
  • Once implanted at the apex, Mtb must engage the host immune response to drive matrix destruction, resulting in cavities within which it proliferates exponentially. (ersjournals.com)
  • Traditional methods of evaluating the status of dental pulp tissue in clinical practice have limitations. (researchsquare.com)