The Pathatrix will selectively bind and purify the target organism from a comprehensive range of complex food matrices (including raw ground beef, chocolate, peanut butter, leafy greens, spinach, tomatoes). (wikipedia.org)
Three methods namely: Rand Water Method 06 (2007), US-EPA Method 1623 (2005) and Drinking Water Inspectorate standard operating procedures (2003) were compared and key different steps in the methods were identified (wrist action speed, centrifuge speed, immunomagnetic separation procedures and addition of pre-treatment steps). (vut.ac.za)
Different experiments were conducted to verify and evaluate the difference between two wrist action shaker speeds, three different centrifuge speeds, two slightly different immunomagnetic separation procedures and when a pre-treatment step was included in the method. (vut.ac.za)
Morphological-based isolation separates CTCs according to size discrepancies, using isolation by size of epithelial tumor cells (ISET) or according to density, using density-gradient separation ( 4 ). (iiarjournals.org)
Methods13
Unlike other detection methods, Pathatrix allows the entire pre-enriched sample or large pooled samples to be recirculated over antibody-coated paramagnetic beads. (wikipedia.org)
In this review, the current and novel methods for detection of breast cancer metastasis, as well as the mechanisms involved in metastasis and the treatment of breast cancer metastasis, are discussed. (iiarjournals.org)
Currently, detection of breast cancer metastasis relies on clinical manifestations of the spread to distant organs, biopsies of affected organs, radiological evaluations, imaging methods and serum tumor markers ( 3 , 4 ). (iiarjournals.org)
Immunological techniques, which are the most widely used methods, employ immunomagnetic isolation ( 4 ). (iiarjournals.org)
After isolation, the source and genetic make-up of CTCs are characterized using nucleic acid-based methods, such as quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR), or cytometric-based methods, such as flow cytometry and enzyme-linked immunospot assay technology ( 4 ). (iiarjournals.org)
There are several different methods for the detection of DTCs and CTCs from bone marrow or blood ( 7 - 9 ). (iiarjournals.org)
Such inactivated reference materials were used as positive controls in assays to detect suspected B. anthracis in samples because live agent cannot be shipped for use in field settings, in improvement of currently deployed detection methods or development of new methods, or for quality assurance and training activities. (cdc.gov)
In this review article, we aimed to present contemporary methods and techniques for the sampling, isolation, and analysis of single cells and to give an overview of the current scientific literature about CRC at the single-cell level. (por-journal.com)
Methods: Identification of tumour-associated endothelial markers (TEM) by comparing antigen expression on normal vs t-CEC and assess the presence of t-CEC in peripheral blood of cancer patients by incorporating TEM in our novel flow cytometry-based CEC detection assay. (researchgate.net)
Molecular techniques have facilitated the identification, genotyping, and monitoring of food contamination sources, identification of genetically modified organisms, and use as food detection methods to avoid falsification through genetic markers. (peertechzpublications.com)
In general, the most known and used methods are Western Blots (WB), Enzyme-Linked Immune Sorbent Assay (ELISA), Lateral-Flow Assay (LFA), and Dot-Blot Assay (DBA) and have been applied in the detection of carcinogenic compounds, pathogenic and allergenic microorganisms using antibodies [8]. (peertechzpublications.com)
in this scenario, careful consideration must be given to the isolation approach, whereas methods that discriminate against certain subpopulations may result in the exclusion of CTCs that carry biological relevance. (oaepublish.com)
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Assay3
2005. Development of a species-specific and sensitive detection assay for Phytophthora infestans and its application for monitoring of inoculum in tubers and soil . (hutton.ac.uk)
Any assay development effort requires testing, evaluation, and validation of the assays with live or inactivated spiking materials in appropriate matrices relevant to the environments in which the assays are intended to be used (e.g., aerosol collection filters, soils, or clinical matrices). (cdc.gov)
Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. (immpact-international.org)
Extracellular matrix3
As a result of changes in their adhesion molecules, the cells separate from the primary cell population and gain the ability to migrate independently within the extracellular matrix and, eventually, outside it (7). (czytelniamedyczna.pl)
Cancerous cells are embedded into diverse tissues consisting of cancer-associated fibroblasts, extracellular matrix, vascular and lymphatic networks, and immune cells, among others. (por-journal.com)
uNK cells isolated from obese women had altered expression of genes and pathways associated with extracellular matrix remodeling and growth factor signaling. (jci.org)
Enrichment2
Fluorescent EV spike recovery evidenced that target EV subpopulations can be efficiently retrieved from plasma, and that their enrichment is dependent not only on complex matrix composition, but also on the EV surface phenotype. (biomedcentral.com)
This method is based on the immunomagnetic enrichment of CTCs from patient's blood samples, with subsequent staining of these cells. (iiarjournals.org)
Antibodies1
Quantification and characterization of exosomal proteins is then performed using appropriate detection antibodies against exosome-associated antigens, which can be either generic or cell / tissue specific exosomes. (immpact-international.org)
Clinical3
The differential clinical value carried within each distinct EV subset highlights the advantages of selective isolation. (biomedcentral.com)
Moreover, neither isolation approach is readily compatible with routine clinical workflows, which is still an underappreciated aspect in studies attempting to harness the potential of EVs for medical diagnostics. (biomedcentral.com)
The increasing isolation of non- albicans species suggests increasing pathogenicity of these species with varying degrees of clinical symptoms [ 7 ] . (encyclopedia.pub)
Quantification3
Expressing a positive analytical measurement falling below the LOQ as less than the established quantification limit or expressing a non-detect result as less than the detection limit. (epa.gov)
In this study, we present a novel nucleic acid-based method for the detection and possible quantification of CTCs utilizing quantitative TaqMan real time PCR. (iiarjournals.org)
The application of molecular techniques in food is large, several authors comment on the detection and quantification of strains of pathogenic microorganisms present in foods and the presence of toxins and waste allergens not related to specific goods in production lines. (peertechzpublications.com)
Quantitative1
Integrated isolation and quantitative analysis of exosome-transmitted proteins and nucleic acids using immunocapsura approaches. (immpact-international.org)
CTCs3
We present a method for the detection of CTCs by real-time PCR on different cytokeratin markers. (iiarjournals.org)
Numerous studies suggest that detection of CTCs in the blood or disseminated tumour cells (DTCs) in the bone marrow of patients with cancer is linked to poor outcomes of the disease and reduced treatment efficiency ( 5 , 6 ). (iiarjournals.org)
Herein, we review the history and the current status of CTC detection, discuss the prognostic, predictive, and diagnostic capabilities of these technologies, and describe application of CTCs as models for the study of tumor biology. (amegroups.org)
Frequency1
Increasing isolation frequency of C. glabrata is associated with old age, as reported by Zhang et al. (encyclopedia.pub)
Pesticides1
The incorporation of nanomaterials provides highly sensitive sensing devices allowing the efficient detection of pesticides. (scirp.org)
Immunoassay1
There are two formats in each immunoassay test: 1) sandwich format (for macromolecule detection, such as foodborne pathogens and allergen proteins). (peertechzpublications.com)
Method3
Rand Water has adopted their method for isolation and detection of Cryptosporidiumoocysts and Giardia cysts in water from United State Environmental Protection Agency (US-EPA) Method 1623, 1999. (vut.ac.za)
Optical, electrochemical and piezoelectric biosensors have been reported based on the detection method. (scirp.org)
Markers1
Plasma-derived EVs were captured and detected using this straightforward protocol, sequentially combining isolation and staining of specific surface markers, such as CD9 or CD41. (biomedcentral.com)
Cells1
Immunomagnetic Isolation of HER2-Positive Breast Cancer Cells Using a Microfluidic Device. (acs.org)
Types2
Three different types of water matrices (reagent grade water, drinking water and raw water) were used for the experiments and secondary validation. (vut.ac.za)
The bedrock of successful biodetection platforms and sensors is use of well-characterized molecular assays, immunoassays, or other types of detection assays. (cdc.gov)
Cell1
In this review article we aimed to emphasize the importance of single-cell analyses by presenting tumor heterogeneity and the limitations of conventional investigational approaches, followed by an overview of the whole single-cell analytic workflow from sample isolation to amplification, sequencing and bioinformatic analysis and a review of recent literature regarding the single-cell analysis of colorectal cancers. (por-journal.com)
System2
Pathatrix is a high volume recirculating immuno magnetic-capture system developed by Thermo Fisher Scientific (and supplier parts by Life Technologies) for the detection of pathogens in food and environmental samples. (wikipedia.org)
The Pathatrix is a microbial detection system that allows for the entire sample to be analyzed. (wikipedia.org)
Samples1
Therefore efforts should be made to improve detection efficiencies from these samples. (iiarjournals.org)
Complex1
Here, we addressed central aspects of EV immunoaffinity isolation from simple and complex matrices, such as plasma. (biomedcentral.com)
Conditions1
Preclinical research on non-invasive biomarkers for the detection and monitoring of a number of pathological conditions (inflammation, cancer, neurodegeneration, etc. (immpact-international.org)
Review1
This review gives an overview of recent advances and new trends in biosensors for pesticide detection. (scirp.org)
Addition1
In addition, ASCO also recommends mammography for the early detection of relapse in breast cancer ( 5 ). (iiarjournals.org)
Cancer1
Detection of breast cancer metastasis at the earliest stage is important for the management and prediction of breast cancer progression. (iiarjournals.org)
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Blood1
Since blood plasma circulates through the whole body, it is often the biofluid of choice for biomarker detection in EVs. (biomedcentral.com)
Journal1
A. Sassolas, B. Prieto-Simón and J. Marty, "Biosensors for Pesticide Detection: New Trends," American Journal of Analytical Chemistry , Vol. 3 No. 3, 2012, pp. 210-232. (scirp.org)
Development1
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