Mass spectrometry subunit ribosomal proteins. Medical search. Frequent questions

Ribosomes bind to messenger RNAs and use their sequences for determining the correct sequence of amino acids to generate a given protein. (wikipedia.org)
A ribosome is made from complexes of RNAs and proteins and is therefore a ribonucleoprotein complex . (wikipedia.org)
The aggregation, arrangement and bonding together of constituent RNAs and proteins to form ribosomal subunits. (yeastrc.org)
Nuclear bodies concentrate specific proteins and RNAs on distinct chromosomal DNA regions to regulate gene expression. (yale.edu)
RNA Polymerase II, for instance, is vital in the production of messenger RNAs that specify the amino acid sequences of each of the proteins in the cell. (phys.org)
In particular, advances and current results of bioinformatics including genome and sequence analysis, protein domains or protein families, large-scale data analysis (e.g. next generation sequences, proteomics data), analysis of different functional RNAs (e.g. miRNAs, lncRNAs) are explained and discussed. (uni-wuerzburg.de)
In addition, the structure and function of an RNA can be modulated by various trans-acting factors, such as proteins, metabolites or other RNAs. (helmholtz-hzi.de)

New discoveries regarding protein complexes, interaction partners, interaction domains, and biological roles of players that are part of these pathways are emerging. (nih.gov)
The mitochondrial ribosomes (mitoribosomes) synthesize, almost exclusively, membrane proteins that are subunits of oxidative phosphorylation complexes. (molbiolcell.org)
Our group discovered the Gcn2 positive effectors Gcn1 and Gcn20 and the importance of ribosomal Gcn1/Gcn20/Gcn2 regulatory complexes in Gcn2 activation. (nih.gov)
FSHD myoblasts exhibited a greater abundance but slower turnover rate of subunits of mitochondrial respiratory complexes and mitochondrial ribosomal proteins, which may indicate an accumulation of 'older' less viable mitochondrial proteins in myoblasts from individuals affected by FSHD. (ljmu.ac.uk)
To dissect the role of RNA-binding proteins in translation and how they modulate RNA structure and stability, it is crucial to study the interplay and mechanical features of these RNA-protein complexes simultaneously. (helmholtz-hzi.de)
This work illustrates how to employ single-molecule-fluorescence-coupled optical tweezers to explore the conformational and thermodynamic landscape of RNA-protein complexes at a high resolution. (helmholtz-hzi.de)
A synthetic peptide library for benchmarking crosslinking-mass spectrometry search engines for proteins and protein complexes. (imp.ac.at)

As with its human and yeast homologs, OGFOD1 and Tpa1p, respectively, we identified Sud1 to catalyze prolyl-hydroxylation of the small ribosomal subunit protein RPS23. (ox.ac.uk)

Here, by employing a systematic mass spectrometry-based approach, we discovered the previously uncharacterized membrane protein Mrx15 that interacts via a soluble C-terminal domain with the large ribosomal subunit. (molbiolcell.org)
Further analysis of the increased number phenotype revealed components of the 60S large ribosomal subunit (RPL proteins) as regulators of CB assembly. (yale.edu)

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. (wikipedia.org)
Mrx15 contacts mitochondrial translation products during their synthesis and plays, together with the ribosome receptor Mba1, an overlapping role in cotranslational protein insertion. (molbiolcell.org)
Proteins involved in protein folding, protein synthesis, protein degradation, ATP supply and structural proteins changed in abundance in response to heat and/or selenium. (nih.gov)
Avilamycin inhibits bacterial protein synthesis by binding to the 50S ribosomal subunit. (genesilico.pl)
In addition to defining new resistance mechanisms, these data refine our understanding of the probable ribosome contacts made by orthosomycins and of how these antibiotics inhibit protein synthesis. (genesilico.pl)
Ribosomes ( / ˈ r aɪ b ə ˌ s oʊ m , - b oʊ -/ ) are macromolecular machines , found within all cells , that perform biological protein synthesis (mRNA translation). (wikipedia.org)
The synthesis of proteins from their building blocks takes place in four phases: initiation, elongation, termination, and recycling. (wikipedia.org)
Inhibits bacterial growth, possibly by blocking dissociation of peptidyl tRNA from ribosomes, causing RNA-dependent protein synthesis to arrest. (medscape.com)
Inhibits bacterial protein synthesis. (medscape.com)
Inhibits protein synthesis by binding to 50S ribosomal subunits, preventing amino acids from being transferred to the growing peptide chains, which in turn inhibits protein synthesis. (medscape.com)
Despite having evolved from Pol II, Pol IV and V do not appear to be involved in protein synthesis, or to be absolutely essential for life. (phys.org)
Protein Synthesis at Atomic Resolution: Mechanistics of Translation in the Light of Highly Resolved Structures for the Ribosome. (mpg.de)
Neomycin sulfate, an aminoglycoside antibiotic , exerts antibacterial activity through irreversible binding of the nuclear 30S ribosomal subunit, thereby blocking bacterial protein synthesis. (medchemexpress.com)

Recombinant AviRa and AviRb proteins retain their activity after purification, and both specifically methylate in vitro transcripts of 23S rRNA domain V. Reverse transcriptase primer extension indicated that AviRa is an N-methyltransferase that targets G2535 within helix 91 of the rRNA, whereas AviRb modified the 2'-O-ribose position of nucleotide U2479 within helix 89. (genesilico.pl)
Each subunit consists of one or more ribosomal RNA (rRNA) molecules and many ribosomal proteins (RPs or r-proteins). (wikipedia.org)
If nucleoli are transient structures designed solely for making ribosome subunits, as was previously believed, they should collapse when rRNA transcription is inhibited. (the-scientist.com)
Subsequently, we used the Gcd− selection to identify domains/residues in eIF2 and tRNAi, eIF1, eIF1A, eIF3, and residues of 18S rRNA located near the 'P' decoding site of the 40S subunit, that participate in rapid TC recruitment in vivo (Figure 1A). (nih.gov)

2. Application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the study of Helicobacter pylori. (nih.gov)
9. Ribosomal subunit protein typing using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification and discrimination of Aspergillus species. (nih.gov)
10. Revisiting Species Identification within the Enterobacter cloacae Complex by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. (nih.gov)
11. Species-specific identification and differentiation of Arcobacter, Helicobacter and Campylobacter by full-spectral matrix-associated laser desorption/ionization time of flight mass spectrometry analysis. (nih.gov)
13. Bacterial Sub-Species Typing Using Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry: What Is Promising? (nih.gov)
15. Matrix-assisted laser desorption ionization (MALDI)-time of flight mass spectrometry- and MALDI biotyper-based identification of cultured biphenyl-metabolizing bacteria from contaminated horseradish rhizosphere soil. (nih.gov)
17. Identification of Cronobacter species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with an optimized analysis method. (nih.gov)
Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)

Ribosomes consist of two major components: the small and large ribosomal subunits. (wikipedia.org)
Ribosomes assemble polymeric protein molecules whose sequence is controlled by the sequence of messenger RNA molecules. (wikipedia.org)
Ribosomes are ribozymes , because the catalytic peptidyl transferase activity that links amino acids together is performed by the ribosomal RNA. (wikipedia.org)
In starvation conditions, the reinitiating ribosomes bypass uORFs 2-4 and reinitiate at GCN4 instead, owing to lowered availability of the ternary complex (TC)-comprised of initiation factor 2 (eIF2), GTP, and initiator Met-tRNAi-which binds to the small (40S) ribosomal subunit to assemble a 43S preinitiation complex (PIC). (nih.gov)

Larval proteins were separated by two-dimensional gel electrophoresis and differential abundances were detected following spot quantitation and hierarchical cluster analysis. (nih.gov)
The protein expression profiles of cytoplasm and membrane/nuclei fractions were checked using protein two-dimensional electrophoresis (2-DE). (hindawi.com)
In the process, we survey experimental techniques for determining protein abundance, principally two-dimensional gel electrophoresis and mass-spectrometry. (biomedcentral.com)
either based on two-dimensional electrophoresis or on mass-spectrometric methods (Table 1 ). (biomedcentral.com)
Determining relative protein expression levels by conventional two-dimensional electrophoresis requires isoelectric focusing, SDS-polyacrylamide gel electrophoresis, staining, fixing, densitometry, and careful matching of the same spots on two or more gels. (biomedcentral.com)
An attractive aspect of this approach is the low capital equipment cost, but a high level of expertise is needed to obtain reproducible gels, and two-dimensional electrophoresis is generally limited to proteins that are neither too acidic, too basic, nor too hydrophobic, and that are between 10 and 200 kDa in size, so that they are reliably separated on gels. (biomedcentral.com)
Two-dimensional fluorescence-difference gel electrophoresis (DIGE) utilizes mass- and charge-matched, spectrally resolvable fluorescent dyes (such as Cy3 and Cy5) to label two different protein samples in vitro prior to two-dimensional electrophoresis. (biomedcentral.com)
Two dimensional gel electrophoresis (2-DE) and mass spectrometry were used to identify proteome alterations in human embryonic cells (HEK-293) following exposure to therapeutic concentrations of MPA. (uni-goettingen.de)
Our preliminary study using 2D isolates were recovered from liq- new cases throughout the world cu- gel electrophoresis in drug sensitive/ uid nitrogen, and sub-cultured in taneous leishmaniasis (CL) remains resistant strains of L. tropica showed RPMI1640 medium (Gibco/BRL) a serious public health problem in that some proteins were differentially supplemented with 10% fetal bovine numerous countries [1,2]. (who.int)

He could then identify peptides from each batch by the offsets in their mass spectra. (the-scientist.com)
Differentially expressed spots are then excised and enzymatically digested, and the resulting peptides are identified using mass spectrometry. (biomedcentral.com)
N-terminal signal peptides facilitate the engineering of PVC complex as a potent protein delivery system. (avalonsoho.com)
use ANTIFREEZE PROTEINS (NM) 1997-2000 MH - Antimicrobial Cationic Peptides UI - D023181 MN - D12.644.350 MN - D24.611.600 MS - Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. (nih.gov)
use ANTIMICROBIAL CATIONIC PEPTIDES (NM) to search MICROBICIDAL CATIONIC PROTEINS 1981-2000 BX - Microbicidal Cationic Proteins FX - Blood Bactericidal Activity MH - Antiretroviral Therapy, Highly Active UI - D023241 MN - E2.319.310.75 MS - Drug regimens, for patients with HIV INFECTIONS, that aggressively supress HIV replication. (nih.gov)
The distinction is that peptides are short and polypeptides/proteins are long. (wikidoc.org)
Conventions== One convention is that those peptide chains that are short enough to be made synthetically from the constituent [[amino acid]]s are called peptides rather than proteins. (wikidoc.org)
and small proteins, such as [[insulin]], can be considered peptides. (wikidoc.org)

Mass spectrometry-based proteomics in combination with subcellular fractionation can provide comprehensive maps of spatio-temporal regulation of protein networks in cells, but involves laborious workflows that does not cover the phospho-proteome level. (nature.com)
ABSTRACT In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. (who.int)
The study shows the usefulness of proteomics in identifying proteins that may express differences between sensitive and resistant L. tropica isolates. (who.int)

The many novel factors and separate classes of proteins identified support the view that the nucleolus may perform additional functions beyond its known role in ribosome subunit biogenesis. (hud.ac.uk)
Engineering and functional analysis of yeast with a monotypic 40S ribosome subunit. (avalonsoho.com)

In the keynote presentation, Dr. Wade Harper described his team's recent discovery of a novel reticulophagy receptor for selective autophagic degradation of the endoplasmic reticulum, and discussed molecular mechanisms involved in ribophagy and non-autophagic ribosomal turnover. (nih.gov)
Finally up regulation of proteins involved in translation and intracellular degradation was also observed. (biomedcentral.com)
An acetylation site in proximity to the protein-specific QTL implied a regulatory function of lysine acetylation in the degradation of the protein. (biomedcentral.com)
A conserved quality-control pathway that mediates degradation of unassembled ribosomal proteins. (deshaieslab.com)

Proteome profiling and global protein-interaction approaches have significantly improved our knowledge of the protein interactomes of autophagy and other cellular stress-response pathways. (nih.gov)
Isotope labeling and mass spectrometry unite to track proteome dynamics. (the-scientist.com)
Although very sensitive and powerful, these techniques lack throughput, as they cannot provide information on protein location at a proteome-wide level. (nature.com)
However, both methods rely on isobaric tandem mass tag (TMT) labeling for quantifying subcellular protein localization, and they require extensive off-line peptide fractionation and consequently lengthy liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis time to achieve the desired depth on the proteome, thus minimizing throughput. (nature.com)
These data highlight the dynamic nature of the nucleolar proteome and show that proteins can either associate with nucleoli transiently or accumulate only under specific metabolic conditions. (hud.ac.uk)

Signaling proteins, usually of low abundance, are particularly hard to track. (the-scientist.com)
Attempts to correlate protein abundance with mRNA expression levels have had variable success. (biomedcentral.com)
We also merge many of the available yeast protein-abundance datasets, using the resulting larger 'meta-dataset' to find correlations between protein and mRNA expression, both globally and within smaller categories. (biomedcentral.com)
Although some of the underlying technology for quantifying protein abundance was introduced almost thirty years ago [ 1 , 2 ], there has recently been a significant increase in the development of new tools. (biomedcentral.com)
D2O) labelling and peptide mass spectrometry to investigate the abundance and turnover rates of proteins in cultured muscle cells from 2 individuals affected by FSHD and their unaffected siblings (UASb). (ljmu.ac.uk)
We measured the abundance of 4420 proteins and the turnover rate of 2324 proteins in each (n = 4) myoblast sample. (ljmu.ac.uk)
Genome-wide eQTL analyses with transcriptome-wide gene expression data revealed 20 cis-acting eQTLs associated simultaneously with mRNA expression, ribosome occupancy, and protein abundance. (biomedcentral.com)
One allele of the eQTL was associated with increased mRNA expression and ribosome occupancy but decreased protein abundance. (biomedcentral.com)
Log2 (ZT12/ZT0) is the base 2 logarithm of the protein abundance ratio between ZT12 and ZT0. (nih.gov)
P-value is from t-statistic of log2(protein abundance) values from the 6 samples. (nih.gov)

The Kriwacki laboratory seeks to understand the molecular basis of regulation of cell division, apoptosis, and ribosome biogenesis, with special emphasis on the role of intrinsically disordered proteins (IDPs) in these vital biological processes. (stjude.org)

40S ribosomal protein SA, FK506-binding protein 10, 65kDa regulatory subunit A of protein phosphatase 2, protein disulfide isomerase, stress-induced-phosphoprotein 1, suppression of tumorigenicity 13 and collagen type II alpha 1, were differentially expressed in high temperature treatment only. (nih.gov)
Proteins differentially expressed between control and SCU-treated group, control and HR group, or HR and SCU + HR group were identified using mass spectrometry (MS/MS). Possible interaction network of these target-related proteins was predicted using bioinformatic analysis. (hindawi.com)
Proteins differentially expressed between control and SCU-treated group and proteins differentially expressed between HR and SCU + HR group were considered as possible target-related proteins of SCU. (hindawi.com)
At the same time, proteins differentially expressed between control and HR group were also identified to provide information about possible proteins involved in HR injury. (hindawi.com)
Cells were treated for 72 hours, and total cell lysate was resolved by 2-DE followed by QTOF-MS/MS analysis of all identified differentially regulated proteins. (uni-goettingen.de)
A total of 12 proteins were differentially regulated in HEK-293 cells following exposure to MPA. (uni-goettingen.de)

This gene encodes a ribosomal protein that is a component of the 40S subunit and a member of the S14P family of ribosomal proteins. (wikipedia.org)
The HTT gene encodes a 350 kDa protein whose exact function is still not understood. (nature.com)

To identify proteins whose expression is modified by these environmental stressors, we performed a proteomic analysis on larval green and white sturgeons exposed to 18 or 26 degrees C and micro-injected with Seleno-L-Methionine to reach 8microgg(-)(1) selenium body burden, with L-Methionine as a control. (nih.gov)
For a comprehensive study of the mechanism of SCU on HCMECs, a proteomic approach (2-DE combined with MS/MS) was used for seeking possible target-related proteins of SCU in HCMECs, both under normal condition and under HR treatment. (hindawi.com)
Using proteomic approach offers us the opportunity to search possible target-related proteins of SCU in an unbiased way. (hindawi.com)
Conclusions: This extensive proteomic analysis shows that nucleoli have a surprisingly large protein complexity. (hud.ac.uk)
Using label-free technology and liquid chromatography-tandem mass spectrometry for proteomic analysis, the effects of microwave drying on sorghum differential protein expression, functional classification, and metabolic pathways were analyzed at the molecular level. (scielo.br)

Treatment with a 2'-O-methoxyethyl modified antisense oligonucleotide targeting exon 3 of the double homeobox 4 (DUX4) transcript tended to reverse mitochondrial protein dysregulation in FSHD myoblasts, indicating the effect on mitochondrial proteins may be a DUX4-dependent mechanism. (ljmu.ac.uk)

The sequence of DNA that encodes the sequence of the amino acids in a protein is transcribed into a messenger RNA chain. (wikipedia.org)

We uncovered the functions of ABCE proteins Rli1/ABCE1 and Arb1 in PIC assembly and ribosome biogenesis, and identified the tRNA methyltransferase Gcd10/Gcd14, which contributed to the discovery of the TRAMP-mediated RNA surveillance pathway. (nih.gov)
Head swivel on the ribosome facilitates translocation by means of intra-subunit tRNA hybrid sites. (mpg.de)

Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1. (tsinghua.edu.cn)
Ten conserved cytoplasmic proteins were more abundant in wt SNs than in the ΔascV mutant, but without any clear association to a secretion system. (biomedcentral.com)

MS analysis of nucleoli isolated from HeLa cells in which transcription had been inhibited showed that a subset of proteins was enriched. (hud.ac.uk)

Differential proteins were significantly (P ˂ 0.01) involved in metabolic pathways, such as carbon metabolism, glycolysis/gluconeogenesis, carbon fixation in photosynthetic organisms, biosynthesis of amino acids, amino sugar and nucleotide sugar metabolism, and the TCA cycle. (scielo.br)

As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (wikipedia.org)
Over 30% of the nucleolar proteins were encoded by novel or uncharacterized genes, while the known proteins included several unexpected factors with no previously known nucleolar functions. (hud.ac.uk)
FOXM1-regulated processes are mediated through the transactivation of key genes by FOXM1 protein binding to target sequences in gene promoters [ 2 ]. (biomedcentral.com)
Some of the Pol IV and Pol V subunits are encoded by the same genes as the corresponding Pol II subunits, but others come from duplicated Pol II subunit genes that have changed over time. (phys.org)
Then in a paper published Dec. 4 in Molecular Cell, the Pikaard lab announced a breakthrough in understanding the phenomenon of nucleolar dominance, the silencing of an entire parental set of ribosomal RNA genes in a hybrid plant or animal. (phys.org)

T3SS is also called "injectisome" because it enables the secretion and simultaneous injection of effector proteins produced in the prokaryotic cytoplasm across the bacterial envelope and then, through a needle and a translocon complex, into the target eukaryotic cells across their membrane [ 17 ]. (biomedcentral.com)

Ribosomal protein eL39 is important for maturation of the nascent polypeptide exit tunnel and proper protein folding during translation. (avalonsoho.com)
Proteins]] are '''polypeptide''' [[molecule]]s (or consist of multiple polypeptide subunits). (wikidoc.org)

2 While many proteins in the nucleolus, including RNA-processing factors and exosome components, did indeed decrease in response to the inhibition, others, such as small nuclear ribonucleoproteins (snRNPs), increased. (the-scientist.com)
The goal of APEX-ChIP is to understand how nuclear bodies interact with chromatin, by biotinylating molecular constituents surrounding the marker protein and thereby enhancing ChIP signals. (yale.edu)
Purification of nuclear localization signal-containing proteins and its application to investigation of the mechanisms of the cell division cycle. (ipracell.be)
revised 9/6/2000) TOTAL DESCRIPTORS = 184 MH - Active Transport, Cell Nucleus UI - D021581 MN - G6.535.166.310.100 MN - G6.535.166.700.100 MS - Gated transport mechanisms by which proteins or RNA are moved across the NUCLEAR MEMBRANE. (nih.gov)

7. Rapid, Sensitive, and Specific Escherichia coli H Antigen Typing by Matrix-Assisted Laser Desorption Ionization-Time of Flight-Based Peptide Mass Fingerprinting. (nih.gov)

We combine a variety of tools such as X-ray crystallography and cryogenic electron microscopy, protein and peptide chemistry, in vitro and in-cell functional assays using a range of biochemical and biophysical techniques, and most recently, high-resolution microscopy. (nih.gov)
These observations, together with those on enzyme homologs described in the companion articles, reveal conserved biochemical and biological roles for a widely distributed ribosomal oxygenase. (ox.ac.uk)
The microbial proteins targeted by the antibiotic are essential components of biochemical reactions in the microbes, and interference with these physiological pathways kills the microorganisms. (mhmedical.com)

In addition, we found down regulation of cytoskeleton, chaperone, signal transduction proteins, and proteins involved in metabolic pathways. (biomedcentral.com)

8. Use of MALDI-TOF mass spectrometry fingerprinting to characterize Enterococcus spp. (nih.gov)
Fifteen of these spots were identified using MALDI TOF/TOF mass spectrometry. (nih.gov)
MALDI mass spectrometry confirmed the exact positions of each of these modifications, and additionally established that a single methyl group is added at each nucleotide. (genesilico.pl)
Use of MALDI-TOF mass spectrometry for identification of bacteria that are difficult to culture. (medscape.com)
Comparison of bacterial identification by MALDI-TOF mass spectrometry and conventional diagnostic microbiology methods: agreement, speed and cost implications. (medscape.com)

Thus, to aid in the functional characterization of these two proteins and identify the biological processes in which these proteins operate, we used immunoprecipitation coupled with mass spectrometry (IP-MS) to identify proteins interacting with AtNHR2A and AtNHR2B and to generate their interactome network. (frontiersin.org)

These immundominant allergens were identified by nanoscale UPLC-MS/MS to be 60S ribosomal protein L10 (25 kDa), electron transfer flavoprotein B subunit (29 kDa), ketol acid reductoisomerase (44 kDa), and heat shock protein 70 (65 kDa). (cdc.gov)

Erythromycin, roxithromycin, and clarithromycin: Use of slow-binding kinetics to compare their in vitro interaction with a bacterial ribosomal complex active in peptide bond formation. (mpg.de)

Tumor cell lines were evaluated using the following tests: trypan blue exclusion test, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], flow cytometry, cytoskeleton organization analysis, cell cycle, mitochondria membrane depolarization, clonogenicity test, DNA fragmentation test and differential protein expression by HPLC-Chip/MS analysis. (biomedcentral.com)
After sorghum was dried using a microwave, 85 differential proteins were identified. (scielo.br)
The up-regulation and down-regulation of differential protein expressions significantly changed them, and proteins with larger up-regulated and down-regulated expressions were postulated to affect biological and metabolic processes of sorghum during subsequent processing. (scielo.br)
Overall, this study showed that among the related metabolic pathways, differential protein changes in glycometabolism pathways may have the greatest impact on metabolic processes. (scielo.br)

The fourth Vancouver Autophagy Symposium showcased research that expands our understanding of the protein interaction networks and molecular mechanisms underlying autophagy and other cellular stress responses in the context of distinct stressors. (nih.gov)
Molecular models of the 50S subunit crystal structure show that the N-1 of the G2535 base and the 2'-hydroxyl of U2479 are separated by approximately 10 A, a distance that can be spanned by avilamycin. (genesilico.pl)
The research revealing the subunit compositions of RNA Polymerases II, IV and V in the plant genus Arabidopsis was published online Dec. 23, 2008 in Molecular Cell. (phys.org)
Optical tweezers are a single-molecule technique that allows probing of intra- and intermolecular interactions that govern complex biological processes involving molecular motors, protein-nucleic acid interactions, and protein/RNA folding. (helmholtz-hzi.de)

measurements taken from mRNA and protein levels are complementary and both are necessary for a complete understanding of how the cell works [ 3 ]. (biomedcentral.com)
Immunoblot analysis revealed that MPA increased MLCK expression both at mRNA and protein levels. (uni-goettingen.de)

Subcellular translocation of a protein is a dynamic regulatory event, and it is therefore essential to incorporate the temporal dimension when studying protein translocation in response to stimuli. (nature.com)

The multiple localizations of AtNHR2A and AtNHR2B suggest that these two proteins are highly dynamic and versatile, likely participating in multiple biological processes. (frontiersin.org)
Moreover, the comprehensive analysis of the AtNHR2A- and AtNHR2B-interacting proteins using published empirical information revealed that the functions of AtNHR2A and AtNHR2B are not limited to plant immunity but encompass other biological processes. (frontiersin.org)
These proteins play an important biological role in m6A modifications (Table 1 , Fig. 1 ). (biomedcentral.com)
Ribosomal protein hydroxylation is emerging as an important 2OG oxygenase catalyzed pathway, but its biological functions are unclear. (ox.ac.uk)

Lamond and Mann have extended their approach to the study of protein turnover within the nucleolus. (the-scientist.com)
Our results highlight the importance of post-transcriptional processes and protein turnover in FSHD pathology and provide a resource for the FSHD research community to explore this burgeoning aspect of FSHD. (ljmu.ac.uk)

We also use p27 as a model system for studies of small molecule/disordered protein interactions. (stjude.org)
Moreover, analysis of the protein interactome of wild-type versus DNA binding deficient FOXM1 shows that the reduced recruitment is not due to inhibition of protein-protein interactions. (biomedcentral.com)
Even in FOXM1 mutants with almost complete loss of binding, the protein-protein interactions and pattern of phosphorylation are largely unaffected. (biomedcentral.com)
Cryo-EM structure of Mycobacterium tuberculosis 50S ribosomal subunit bound with clarithromycin reveals dynamic and specific interactions with macrolides. (avalonsoho.com)

HN - 2001 BX - Vaccines, Anthrax MH - Antifreeze Proteins UI - D021301 MN - D12.776.53 MS - Proteins that bind to ice and modify the growth of ice crystals. (nih.gov)
HN - 2001 MH - Antifreeze Proteins, Type II UI - D021341 MN - D12.776.53.200 MS - A subclass of ANTIFREEZE PROTEINS that have a cystine-rich globular structure of approximately 14 kD. (nih.gov)
HN - 2001 MH - Antifreeze Proteins, Type III UI - D021343 MN - D12.776.53.350 MS - A subclass of ANTIFREEZE PROTEINS that are globular, 6.5 kD in size and contain compact beta-sheet structures. (nih.gov)
HN - 2001 MH - Antifreeze Proteins, Type IV UI - D021302 MN - D12.776.53.500 MS - A subclass of ANTIFREEZE PROTEINS that contain four amphathic alpha-helices folded into an antiparallel helix bundle. (nih.gov)

Here we demonstrated that IL15-conditioned T cells were primed by the metabolic energy sensor AMP-activated protein kinase to undergo diminished translation relative to effector T cells. (aacrjournals.org)
However, we showed that IL15-conditioned T cells exhibited a remarkable capacity to enhance their protein translation in tumors, which effector T cells were unable to duplicate. (aacrjournals.org)
Once injected in the eukaryotic cytosol, effector proteins are able to modulate cell signalling pathways, or alternatively disrupt the dynamics of the cytoskeleton, thereby modulating host cell biology for the benefit of the pathogen [ 17 ]. (biomedcentral.com)

CBs are necessary for normal development, since vertebrate embryos depleted of the CB scaffolding protein coilin do not properly process mRNA and cannot support growth. (yale.edu)
Cajal bodies are normally made up of two subunits, a coilin containing domain and an SMN containing domain. (yale.edu)

The data also show that the protein composition of nucleoli is not static and can alter significantly in response to the metabolic state of the cell. (hud.ac.uk)
After either codepletion of SENP3 and SENP5 or depletion of B23/nucleophosmin, we observed accumulation of SUMO proteins within nucleoli. (rupress.org)

In 2002, Mann and erstwhile colleague Angus Lamond of the University of Dundee in Scotland had used SILAC to map several hundred proteins in the nucleolus. (the-scientist.com)
Background: The nucleolus is a subnuclear organelle containing the ribosomal RNA gene clusters and ribosome biogenesis factors. (hud.ac.uk)

Immunostained P. variotii antigens were excised and sequenced using nanoscale Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry (nanoscale UPLC-MS/MS). De-identified sera from 6 healthcare worker s (HCW) and 2 non-atopics were used as controls. (cdc.gov)
Analyzing purified Pol IV and Pol V by a sophisticated technique known as tandem mass spectrometry, the Pikaard lab and a team of collaborators at Pacific Northwest National Laboratory, led by Ljiljana Paa-Toli, discovered 12 subunits in both Pol IV and Pol V that correspond one-for-one to the 12 subunits of Pol II. (phys.org)
Whole right kidney samples from mice were obtained at ZT12 (n=3) and ZT0 (n=3) and processed for quantitative protein mass spectrometry using tandem mass tagging (TMT) for quantification. (nih.gov)

Taken together, our data reveal how these ribosome receptors organize membrane protein biogenesis in mitochondria. (molbiolcell.org)

Currently, my lab and I are examining the structural and chemical biology of membrane proteins. (nih.gov)
We are currently pursuing studies in two different directions: 1) membrane-embedded enzymes and proteins that use lipids as substrates and 2) transporters that move transition metal ions across the membrane into the mitochondria. (nih.gov)
Structural insights into the membrane microdomain organization by SPFH family proteins. (avalonsoho.com)

Dynamic change in subcellular localization of signaling proteins is a general concept that eukaryotic cells evolved for eliciting a coordinated response to stimuli. (nature.com)

Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. (who.int)

The results indicated that proteins such as p27BBP protein (EIF6), heat shock 60 kDa protein 1 (HSPD1), and chaperonin containing TCP1 subunit 6A isoform (CCT6A) might play important roles in the effects of SCU. (hindawi.com)
As an example, the interaction of the SARS-CoV-2 programmed ribosomal frameshifting element with the trans-acting factor short isoform of zinc-finger antiviral protein is elaborated. (helmholtz-hzi.de)

Pathologically, FXTAS is characterized by ubiquitin-positive intranuclear neuronal inclusions, raising the possibility that failure of protein quality control pathways could contribute to disease pathogenesis. (sdbonline.org)
Impairment of protein quality control pathways is a common feature among almost all neurodegenerative conditions, including Alzheimer's disease, Parkinson's disease, polyglutamine diseases and ALS/frontotemporal dementia. (sdbonline.org)
Together, these conditions have been referred to as neurodegenerative proteinopathies, implying that failures in protein quality control pathways are a proximal shared event in disease pathogenesis and thus a potentially shared route to effective therapeutics. (sdbonline.org)
Further validation of three of the identified proteins provided new insights into specific pathways and processes related to plant immunity where AtNHR2A and AtNHR2B participate. (frontiersin.org)

The mHTT protein influences a plethora of cellular processes including transcription, axonal transport, cytoskeletal structure/function, signal transduction, and autophagy that ultimately result in cell death. (nature.com)
We are obtaining detailed atomic descriptions of the proteins, connecting their structural chemistry with their cellular function in healthy human beings, and trying to understand how structural and functional aberrations are linked to human diseases. (nih.gov)
Finally, we investigate the spatio-temporal stress signaling, revealing cellular relocation of ribosomal proteins in response to hypertonicity and muscle contraction. (nature.com)

to others, the microsomes consist of protein and lipid contaminated by particles. (wikipedia.org)

This is the first study to demonstrate regulation of CBs by ribosomal proteins. (yale.edu)
Both proteins showed up-regulation at mRNA as well as at protein level following MPA exposure.MLC2 is known to be involved in several functions including tight junctions (TJ) regulation. (uni-goettingen.de)

To characterize the dynamics of growth factor signaling, Mann stimulated isotope-tagged batches of cells with EGF for different time intervals and analyzed the combined lysate using mass spectrometry. (the-scientist.com)

Current techniques of Metabolomics and Bioanalytics including quantitative metabolite analysis methods (chromatography, mass spectrometry) are applied for comprehensive gene function- or stress response analyses. (uni-wuerzburg.de)

Because the detailed functions of AtNHR2A and AtNHR2B are still unknown, the main objective of this work is to gain insight into their function by investigating their respective protein-protein interaction network (interactome). (frontiersin.org)
For the protein interaction network, glyceraldehyde-3-phosphate dehydrogenase of the down-regulated proteins was postulated to be the key factor affecting the entire metabolic system or signal transduction pathway. (scielo.br)

16. Fingerprinting of Helicobacter pylori strains by matrix-assisted laser desorption/ionization mass spectrometric analysis. (nih.gov)

AtNHR2A ( Arabidopsis thaliana nonhost resistance 2A) and AtNHR2B ( Arabidopsis thaliana nonhost resistance 2B) are two proteins that participate in nonhost resistance, a broad-spectrum mechanism of plant immunity that protects plants against the majority of potential pathogens. (frontiersin.org)

Anatomy2

Organisms2

Diseases1

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes12

Disciplines and Occupations1

Information Science3

RNAs7

Complexes7

Small-ribosomal-subunit1

Large ribosomal subunit2

Synthesis13

RRNA4

Time-of-flight ma8

Ribosomes4

Electrophoresis9

Peptides8

Proteomics3

Ribosome subunit2

Degradation4

Proteome5

Abundance10

Intrinsically disordered proteins1

Differentially6

Gene encodes2

Proteomic5

Mitochondrial proteins1

Encodes1

TRNA2

Cytoplasmic2

Subset of proteins1

Amino1

Genes5

Cytoplasm1

Polypeptide2

Nuclear4

Escherichia1

Biochemical3

Involved in metabolic pathways1

MALDI5

Identify proteins1

Electron1

Peptide bond1

Differential4

Molecular4

MRNA and protein levels2

Regulatory1

Biological4

Turnover2

Interactions4

20014

Effector3

Coilin2

Nucleoli2

Nucleolus2

Tandem mass3

Biogenesis1

Membrane3

Eukaryotic1

Disulfide isomerase1

Isoform2

Pathways4

Cellular3

Consist1

Regulation2

Lysate1

Chromatography1

Interaction network2

Spectrometric1

Pathogens1