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Mass SpectrometryTandem Mass SpectrometryCarbohydratesSpectrometry, Mass, Electrospray IonizationCarbohydrate MetabolismSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationDietary CarbohydratesGas Chromatography-Mass SpectrometryChromatography, LiquidCarbohydrate SequenceChromatography, High Pressure LiquidProteomicsMolecular Sequence DataCarbohydrate ConformationSpectrometry, Mass, Secondary IonAmino Acid SequenceProteomeSpectrometry, Mass, Fast Atom BombardmentOligosaccharidesElectrophoresis, Gel, Two-DimensionalPolysaccharidesPeptidesGlycosylationMagnetic Resonance SpectroscopyDeuterium Exchange MeasurementMolecular StructureIsotope LabelingPeptide MappingReproducibility of ResultsReference StandardsProteinsCalibrationLectinsChromatography, Thin LayerElectrophoresis, Polyacrylamide GelDeuteriumBody Mass IndexMolecular WeightAntigens, Tumor-Associated, CarbohydratePeptide FragmentsSensitivity and SpecificityChromatography, GasMonosaccharidesSolid Phase ExtractionIonsProtein BindingLimit of DetectionGlycoproteinsAmino AcidsStarchGlycopeptidesMetabolomicsProtein Processing, Post-TranslationalTrypsinOxidation-ReductionMannoseSubstance Abuse DetectionCattleFourier AnalysisDisaccharidesIndicators and ReagentsGlucoseModels, MolecularGlycoside HydrolasesMetabolomeFucoseRecombinant ProteinsBacterial ProteinsChromatography, AffinityKineticsIsotopesCyclotronsGlycomicsBinding SitesTime FactorsGalactoseAnalytic Sample Preparation MethodsHydrolysisDietary FatsGlycosphingolipidsGlycolipidsAcetylglucosamineHydrogenIsomerismFatty AcidsChromatography, Ion ExchangeLipidsBlood GlucoseSequence Analysis, ProteinCarbon IsotopesElectrophoresis, CapillaryLiverSubstrate SpecificityDietary ProteinsPlant LectinsHydrogen-Ion ConcentrationProtein ConformationEnergy IntakeSequence Homology, Amino AcidChromatography, Gel
Tandem mass spectroProteomicsAmerican Society forMetabolitesLiquid-chromatograpChromatography and mass spectrometryLipidsProteinsIsotopePeptidesMetabolismMobility-Mass SpectrometryUltra-High-performance LiNuclear magneticCapillary electrophoresisEnzymesBiomoleculesGlycan AnalysisOligosaccharidesFourierBiochemistryCharacterizationMetabolicHPLCStructuresAnalysisSpectroscopicAnalyzeSequenceIonizationOligosaccharideProteinAminoConformationSpectroscopyMethodsAcidsComplexWidelyMammalianAnalyticalMeasurement
Tandem mass spectro3
Proteomics2
American Society for1
Metabolites3
  • 24 hours later, the lungs were excised, flushed with PBS to remove blood and analyzed via liquid-chromatography or gas-chromatography coupled to mass spectrometry for metabolites. (nih.gov)
  • For the purposes of this solicitation, metabolites include small molecules that are the products and intermediates of metabolism, but also carbohydrates, peptides, and lipids. (nih.gov)
  • This has proved useful in metabolomics studies, such as performing metabolic profiling on blood and brain tissue samples and classifying metabolites such as oligonucleotides, carbohydrates, and lipids. (chromatographyonline.com)
Liquid-chromatograp3
Chromatography and mass spectrometry2
Lipids3
Proteins5
Isotope3
  • This publication describes an isotope dilution Ultra-High-performance Liquid Chromatography tandem mass spectrometric method (UHPLC-MS/MS) that was developed and validated for the simultaneous quantification of these impurities in both beverages/liquids and foods. (nih.gov)
  • Hydrogen/deuterium exchange (HDX) is the most widespread implementation of isotope exchange for mass spectrometry, while oxygen 16 O/ 18 O exchange is not applied as frequently as HDX. (acs.org)
  • Liquid chromatography/isotope ratio mass spectrometry (LC/IRMS) is used to analyze various types of samples, including foodstuffs, to determine their authenticity and trace their origin on the basis of their stable carbon isotope ratios (δ13C). (eurekamag.com)
Peptides2
  • 6. Sialylation analysis of O-glycosylated sialylated peptides from urine of patients suffering from Schindler's disease by Fourier transform ion cyclotron resonance mass spectrometry and sustained off-resonance irradiation collision-induced dissociation. (nih.gov)
  • She is an expert on IR action spectroscopy combined with mass spectrometry for structural characterization of biomolecules such as peptides and carbohydrates. (rsc.org)
Metabolism5
  • These included biochemicals related to carbohydrate and lipid metabolism, which were each increased in lungs of obese versus lean mice. (nih.gov)
  • The basic aspects of modeling will be considered, as well as specific applications in the study of carbohydrate, fat, protein metabolism and energy balance. (nih.gov)
  • Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. (nih.gov)
  • Glycogen Storage Diseases Glycogen storage diseases are carbohydrate metabolism disorders. (msdmanuals.com)
  • Galactosemia Galactosemia is a carbohydrate metabolism disorder caused by inherited deficiencies in enzymes that convert galactose to glucose. (msdmanuals.com)
Mobility-Mass Spectrometry4
  • Scientists of the Max Planck Society and of the Freie Universität Berlin developed a novel, highly sensitive and fast method for unambiguous determination of the composition, connectivity and configuration of glycans by ion mobility-mass spectrometry (IM-MS) in negative ionization mode. (efficient.it)
  • A group of scientists created a minireview of the versatile nature of ion mobility-mass spectrometry (IM-MS), and how the technique can be used in different scenarios. (chromatographyonline.com)
  • Ion mobility-mass spectrometry (IM-MS) is becoming a popular method for usage in proteomic studies. (chromatographyonline.com)
  • Eeltink, S. Exploiting Ion-Mobility Mass Spectrometry for Unraveling Proteome Complexity. (chromatographyonline.com)
Ultra-High-performance Li1
  • Liver tissue was collected at 4, 12, and 24 weeks to assess global small molecule metabolite changes using high resolution accurate mass spectrometry coupled to ultra-high-performance liquid chromatography. (cdc.gov)
Nuclear magnetic2
  • The team carried out a detailed analysis of molecular changes in soil organic matter using a combination of gas chromatography/mass spectrometry, nuclear magnetic resonance, and total organic carbon analysis. (nih.gov)
  • Researchers have been able to analyze and comprehend the structure and function of glycans at molecular level due to rising accessibility of cutting-edge technologies including mass spectrometry and Nuclear Magnetic Resonance (NMR) spectroscopy. (reportsanddata.com)
Capillary electrophoresis1
Enzymes3
  • Glycan libraries, glyco-enzymes, and carbohydrate-based biochemicals. (nih.gov)
  • Pathway analysis revealed enrichment of carbohydrate metabolic enzymes in high-risk individuals. (nih.gov)
  • Enzymes are used to analyze structures of carbohydrates and their affinity for carbs makes these a valuable tool for glycomics research. (reportsanddata.com)
Biomolecules2
  • Carbohydrates are the most abundant biomolecules on earth and serve many functions, including structure, nutrition and information transfer 1 . (nature.com)
  • 11. Intact and top-down characterization of biomolecules and direct analysis using infrared matrix-assisted laser desorption electrospray ionization coupled to FT-ICR mass spectrometry. (nih.gov)
Glycan Analysis1
  • Dr Radoslaw Kozak , Head of Glycan Analysis Services, will be attending BioProcess International Conference & Exhibition on September 19th-21st 2023 in Boston , Massachusetts, USA. (ludger.com)
Oligosaccharides3
  • In this chapter, the advanced extraction techniques for fractionation and analytical methodologies applied for the determination of different carbohydrates of milk (lactose, lactulose, and oligosaccharides) are summarized. (intechopen.com)
  • Glycomic platform: Carbohydrates analysis will be performed to determine the free monosaccharides, disaccharides, oligosaccharides, and polysaccharide compositions of the foods. (usda.gov)
  • Mass spectrometry-based (MS) techniques are powerful tools and have been used successfully for the characterization of glycans, but even with the extensive application of tandem MS techniques, it is still extremely difficult from MS data alone to establish anomerity and branching configuration forms of complex oligosaccharides. (nih.gov)
Fourier11
  • 1. Direct characterization of intact polypeptides by matrix-assisted laser desorption electrospray ionization quadrupole Fourier transform ion cyclotron resonance mass spectrometry. (nih.gov)
  • 2. Carbohydrate analysis by desorption electrospray ionization fourier transform ion cyclotron resonance mass spectrometry. (nih.gov)
  • 4. A high-resolution scanning microprobe matrix-assisted laser desorption/ionization ion source for imaging analysis on an ion trap/Fourier transform ion cyclotron resonance mass spectrometer. (nih.gov)
  • 5. Calibration laws based on multiple linear regression applied to matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry. (nih.gov)
  • 7. A new and sensitive on-line liquid chromatography/mass spectrometric approach for top-down protein analysis: the comprehensive analysis of human growth hormone in an E. coli lysate using a hybrid linear ion trap/Fourier transform ion cyclotron resonance mass spectrometer. (nih.gov)
  • 9. Laccase-catalyzed polymerization of two phenolic compounds studied by matrix-assisted laser desorption/ionization time-of-flight and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry with collision-induced dissociation experiments. (nih.gov)
  • 10. Electrospray ionization fourier transform ion cyclotron resonance mass spectrometry of human alpha-1-acid glycoprotein. (nih.gov)
  • 12. A combined ion source for fast switching between electrospray and matrix-assisted laser desorption/ionization in Fourier transform ion cyclotron resonance mass spectrometry. (nih.gov)
  • 14. Peptide analysis: solid phase extraction-elution on diamond combined with atmospheric pressure matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry. (nih.gov)
  • 15. Instrumentation and method for ultrahigh resolution field desorption ionization fourier transform ion cyclotron resonance mass spectrometry of nonpolar species. (nih.gov)
  • The analysis involves comprehensive glycomic and proteomic profiling employing advanced separations methods including nanoflow and microflow liquid chromatography and a host of mass spectrometry platforms including quadrupole time of flight (QTOF), triple quadrupole (QQQ), and Fourier transform mass spectrometry (FTMS-orbitrap). (usda.gov)
Biochemistry1
  • Mass spectrometry (MS) is a versatile technique used in chemistry and biochemistry to analyze the chemical and physical properties of molecules. (facts.net)
Characterization1
Metabolic1
  • The high-carbohydrate, high-fat diet-fed rats showed the symptoms of metabolic syndrome leading to cardiovascular remodeling and nonalcoholic fatty liver disease. (edu.au)
HPLC2
  • After isolation of the tryptic glycopeptide and liberation of the N-linked carbohydrates by polypeptide:N-glycosidase F, 6 major oligosaccharide fractions were separated by HPLC on NH2-bonded phase. (nih.gov)
  • Our research is supported by the Centre for Chemical and Materials Analysis , which offers high quality spectroscopic analysis, including NMR, Mass Spectrometry, X-ray Diffraction and HPLC. (birmingham.ac.uk)
Structures5
  • Carbohydrates form one of the major classes of biological macromolecules in living organisms, but structural analysis and the quality control of carbohydrate structures represent a major challenge due to the frequent occurrence of isomers that are difficult to distinguish using established techniques. (efficient.it)
  • The availability of reliable, robust, rapid and sensitive workflows and technologies for sample preparation, data acquisition and automated analysis that can be handled on a routine basis would be key for the analysis of complex carbohydrate structures in the context of quality control or diagnostic tools. (efficient.it)
  • The carbohydrate structures of a genetically engineered human tissue plasminogen activator variant bearing a single N-glycosylation site at Asn 448 are reported. (nih.gov)
  • Their structures were determined by compositional and methylation analyses combined with fast atom bombardment mass spectrometry. (nih.gov)
  • Glycoproteomic analyses will be performed on samples (diet, feces, intestinal mucin,etc) obtained in experiments designed to examine the impact of specific diets with different carbohydrate structures (e.g. resistant starch) on the microbiome and GI function/immunity. (usda.gov)
Analysis7
  • Mass spectrometry software is software used for data acquisition, analysis, or representation in mass spectrometry. (wikipedia.org)
  • The main aim of this contribution is to provide the reader with a broad view on the recent fractionation and analytical techniques employed for the analysis of carbohydrates in dairy foods and their applications in food and pharmaceutical industry. (intechopen.com)
  • Summary: This market analysis was carried out to provide business information to developers, manufacturers and suppliers in the mass spectrometry field. (azooptics.com)
  • A competitive market analysis of current practices and future developments across 25 key market areas in the mass spectrometry field. (azooptics.com)
  • Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. (nih.gov)
  • We specialize in glycosylation analysis using chromatographic and mass spectrometric techniques and a combination thereof. (ludger.com)
  • Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update for 2017-2018. (bvsalud.org)
Spectroscopic1
  • Her work focuses predominantly on the understanding of the complex mechanism of amyloid-forming polypeptides related to pathogenic neurodegenerative diseases and functional amyloids by advancing mass spectrometry and spectroscopic methods. (rsc.org)
Analyze1
Sequence1
  • We start our exploration of this bottom-up approach using rules developed by a domain expert for recommending the Carbohydrate Sequence heading. (nih.gov)
Ionization1
  • We characterized isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (Bruker Daltonik GmbH, Bremen, Germany) and serotyping (French National Reference Center for Salmonella ). (cdc.gov)
Oligosaccharide1
  • Tandem exoglycosidase digestion has been therefore considered as an orthogonal technique of mass spectrometry to characterize oligosaccharide structure. (nih.gov)
Protein1
  • Complex carbohydrates called glycans play key roles in numerous biological processes, including protein folding, immune system function, and Cell Signaling . (reportsanddata.com)
Amino1
Conformation1
  • Gas-Chromatography/Mass Spectrometry screening was conducted to identify the adulterants and conformation of heroin. (journalcra.com)
Spectroscopy1
Methods2
  • Physical and chemical properties of carbohydrates determine the methods that can be used for their fractionation and purification. (intechopen.com)
  • and by providing mass-structure correlation trend lines for class identifications not possible by other methods (2). (chromatographyonline.com)
Acids4
  • For example, determining the δ13C values of the organic acids in honey is complicated by the presence of large amounts of carbohydrates. (eurekamag.com)
  • In this method, the organic acids in the samples were first separated from the carbohydrates by a size-exclusion column, and then the organic acids were separated from each other by a reverse-phase column connected to the first column via a switching valve. (eurekamag.com)
  • By means of this method, the δ13C values for three organic acids in high-carbohydrate-content simulated honey samples could be determined with high accuracy and precision (≤0.3‰ and ≤0.1‰, respectively). (eurekamag.com)
  • Tissue harvested following each experimental phase was analyzed for nonacid cations, nonstructural carbohydrates, and several organic acids using gas chromatography-mass spectrometry. (cornell.edu)
Complex3
  • Combined with a CCS-database this new IM-MS-based method is a powerful tool that drastically improves the accessibility to structural information of complex carbohydrates. (efficient.it)
  • Seventy percent of the carbohydrates were of the biantennary complex type with fucose at the proximal GlcNAc and zero, one or two alpha 2-3 linked NeuAc. (nih.gov)
  • Mass spectrometry excels at analyzing complex mixtures of compounds. (facts.net)
Widely2
  • Mass spectrometry (MS) is a technique widely implemented for the measurement of environmental pollutants. (dissertations.se)
  • Mass spectrometry is widely used in the pharmaceutical industry for drug discovery and development. (facts.net)
Mammalian1
  • Lactose is the principal carbohydrate present in milk obtained from all mammalian species, such as cow, buffalo, goat, and sheep. (intechopen.com)
Analytical3
Measurement1
  • there is some discussion in the literature) might not require Tg measurement by mass spectrometry, because current guidelines suggest further work-up may be necessary above this threshold. (testcatalog.org)