DifferentiationLineageYolk Sac TMesodermal and endodermalSinus tumourChoriocarcinomaEmbryonicTumor markerAlpha-fetoproteinGerm cell tProteinEndodermPancreaticEmbryonal carcinomaPluripotency markersDiagnosisCarcinomasHESCsDerivativesCellsEpithelialGenesTeratomaOvaryPrimitiveSerumBiopsyOvarianLungCellTerritoriesImmunohistochemistryEpitheliumIntestinalSpecificExpressionRecurrenceProteinsEarlyClinicalPatientsDiseaseDevelopmentMutant
Differentiation16
- After differentiating the mutant and corrected cells into lung organoids, we show expression of SFTPB mRNA during endodermal and organoid differentiation but the protein product only after organoid differentiation. (nature.com)
- Deep sequencing followed by abundancy ranking pinpointed six top hit genes potentially associated with either improved or impaired endodermal differentiation, which were selected for functional validation in CRISPR-Cas9 mediated knockout (KO) lines. (mdpi.com)
- After two days of culture in HSM, the expression levels of α-fetoprotein (AFP) and albumin, which are hepatocyte markers, were upregulated, suggesting that hepatocyte differentiation was initiated ( 19 ). (spandidos-publications.com)
- The protein is an early marker of endodermal differentiation of embryonic stem cells and may play a role in the development of the brain and the sensory organs. (thermofisher.com)
- The murine embryonic stem (ES) cell line, ES-D3 was used in this project as a model for (a) investigating controversial aspects of current protocols used for the derivation of (3 cell types from ES cells and (b) investigating the potential to regulate the differentiation of ES cells into endodermal derivatives 1 e 0-1 ike cells, using extra-cellular factors previously associated with various aspects of pancreatic development. (dcu.ie)
- Treatment of these aggregates with retinoic acid (RA) was previously shown to induce endodermal differentiation We exposed RA_EBs to secondary treatment with agents associated with pancreatic endocrine differentiation, e g Activin A (AA), Sodium Butyrate (SB) and Betacellulin (BTC), with the aim of developing a novel protocol for the regulated derivation of insulin-expressing cell types. (dcu.ie)
- Furthermore, the induction of collective cell migration at the colony edge using an endodermal induction media enhanced endodermal differentiation efficiency in association with cadherin switching, which is involved in the epithelial-mesenchymal transition. (biomedcentral.com)
- Cadherin switching during collective cell migration enhances endodermal differentiation. (biomedcentral.com)
- Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. (harvard.edu)
- In a screen of 4000 compounds, two cell-permeable small molecules were indentified that direct differentiation of ESCs into the endodermal lineage. (harvard.edu)
- First, we show that Sonic Hedgehog promotes cell survival prior to cartilage differentiation through immunostaining for Caspase-9, an apoptosis marker. (duke.edu)
- We further discovered GAPDH as a key glycolytic enzyme regulated by crotonylation during endodermal differentiation from hESCs. (bioinfor.com)
- Crotonylation of GAPDH decreased its enzymatic activity thereby leading to reduced glycolysis during endodermal differentiation from hESCs. (bioinfor.com)
- Inhibition of Brd4:histone H4 interaction by the BET domain inhibitor (+)-JQ1 in ESCs results in enhanced differentiation to the endodermal lineage, by disrupting the protein abundance dynamics. (biomedcentral.com)
- We studied 3 pairs of in-house derived hESC lines for their differentiation capacity towards the mesodermal lineage (osteoprogenitor-like cells) and endodermal lineage (definitive endoderm), as well as after embryoid body formation. (vub.be)
- Moreover, the mutant lines consistently expressed low levels of pluripotency markers (POU5F1 and NANOG) after differentiation excluding an influence of the extra copy(ies) of ID1. (vub.be)
Lineage5
- In Xenopus embryos, enforced expression of Tbx20a, but not Tbx20b, led to induction of mesodermal and endodermal lineage markers as well as cell migration, indicating that the long Tbx20a isoform uniquely bears functional domains that can alter gene expression and developmental behaviour in an in vivo context. (nih.gov)
- The resulting differentiated populations were analysed for transcript expression of a range of multi-lineage markers i e neuronal, myogenic, exocrine and endocrine pancreas, extra-embryonic and apoptotic markers. (dcu.ie)
- Here, we developed an hiPSC-based model to study the effect of collective cell migration in meso-endodermal lineage segregation and cell fate decisions through the control of space confinement using a detachable ring culture system. (biomedcentral.com)
- Through morphological changes, qPCR of pluripotent and germ layer-specific gene markers and flow cytometry analysis, we determined that the induced crotonylation resulted in hESC differentiating into the endodermal lineage. (bioinfor.com)
- We found that induced crotonylation in hESCs resulted in hESCs of different pluripotency states differentiating into the endodermal lineage. (bioinfor.com)
Yolk Sac T3
- What are the findings and markers for yolk sac tumor? (rahulgladwin.com)
- 5 Talerman A, Haije WG, Baggerman L. Serum alphafetoprotein (AFP) in diagnosis and management of endodermal sinus (yolk sac) tumor and mixed germ cell tumor of the ovary. (autopsyandcasereports.org)
- Malignant sacrococcygeal teratoma-endodermal sinus, yolk sac tumor-in infants and children: a 32-year review. (autopsyandcasereports.org)
Mesodermal and endodermal2
- E-PZ-iPS-like cells demonstrated pluripotency by differentiating into ectodermal, mesodermal, and endodermal cells in vitro, although lack of teratoma formation in vivo and incomplete demethylation of pluripotency genes suggested only partial reprogramming. (nih.gov)
- In ectodermal explants, high Activin concentrations induced the expression of Azlocillin sodium salt mesodermal and endodermal markers in the blastula but not gastrula stage. (globaltechbiz.com)
Sinus tumour2
- Ten cysts were malignant, including five granulosa cell tumours, one yolk sac tumour, one endodermal sinus tumour and one dysgerminoma. (edgehill.ac.uk)
- 3 Teilum G. Classification of endodermal sinus tumour (mesoblatoma vitellinum) and so-called "embryonal carcinoma" of the ovary. (autopsyandcasereports.org)
Choriocarcinoma3
- 6 , 8 ] Prognosis was poor for patients with large tumors when more than one-third of the tumor was composed of endodermal sinus elements, choriocarcinoma, or grade 3 immature teratoma. (kaiserpermanente.org)
- Dysgerminoma may be associated with choriocarcinoma or endodermal sinus tumor. (rajeevclinic.com)
- Chronic stress leads to unopposed adductor action of oestrogen to produce choriocarcinoma endodermal sinus pattern: Characterized by tender points can be engaged and therefore are negative for melanocytic markers with genetic overlap with carcinoma of the following points may be useful. (familytreecounseling.com)
Embryonic1
- In mice, morphogenesis of the intestinal epithelium begins around embryonic day 14 (E14), followed by the reshaping of the endodermal pseudostratified epithelium to organize the villi and intervillous region (Grosse, et al. (researchsquare.com)
Tumor marker11
- A tumor marker is a biomarker found in blood, urine, or body tissues that can be elevated by the presence of one or more types of cancer. (wikipedia.org)
- Although mammography, ultrasonography, computed tomography, magnetic resonance imaging scans, and tumor marker assays help in the staging and treatment of the cancer, they are usually not definitive diagnostic tests. (wikipedia.org)
- The use of these tests without understanding their utility has resulted in inappropriate use of tumor marker blood tests, which has resulted in inappropriate over-investigation for cancers. (wikipedia.org)
- If repeated measurements of tumor marker are needed, some clinical testing laboratories provide a special reporting mechanism, a serial monitor that links test results and other data pertaining to the person being tested. (wikipedia.org)
- Interlaboratory proficiency testing for tumor marker tests, and for clinical tests more generally, is routine in Europe and an emerging field in the United States. (wikipedia.org)
- Normalization of tumor marker values may indicate cure despite radiographic evidence of persistent disease. (medscape.com)
- Sometimes, tumor marker values may rise after effective treatment (due to cell lysis), but the increase may not portend treatment failure. (medscape.com)
- A consistent increase in a tumor marker value, combined with lack of clinical improvement, may indicate treatment failure. (medscape.com)
- The usefulness of a tumor marker is in its sensitivity and specificity, as well as its influence on patient management decisions. (medscape.com)
- In addition, beta-hCG serves as an ideal tumor marker for monitoring gestational trophoblastic disease and has set the standard with which other assays must be compared. (medscape.com)
- They increase the tumor marker protein called alpha fetoprotein as well as HCG. (planetayurveda.net)
Alpha-fetoprotein2
- Beta-hCG and alpha-fetoprotein have proved to be useful markers for ovarian germ cell tumors. (medscape.com)
- Tumor markers, including Ca-125, alpha-fetoprotein, lactate dehydrogenase (LDH), and beta-human chorionic gonadotropin (beta-hCG) may be evaluated before surgery. (medscape.com)
Germ cell t1
- Malignant ovarian germ cell tumors: identification of novel prognostic markers and long-term outcome after multimodality treatment. (kaiserpermanente.org)
Protein5
- A five-antibody custom, ready-to-go protein panel, with receptor, protease, and viral markers is available through the GeoMx Technology Access Program or for order through Abcam. (bioinnovations.in)
- NanoString scientists can recommend commercially-available markers for lung epithelium, nasal epithelium, immune response markers, and the viral spike protein. (bioinnovations.in)
- These constructs were overexpressed in the endoderm of Xenopus embryos and protein activity was induced before or after specification of endodermal precursor cells. (uni-goettingen.de)
- In pet cap assays, excitement of either from the inducible chimeric protein (HAIRY2A or MSX-1) with Dex resulted in upregulation of and created a reduction in manifestation and improved the manifestation from the NC marker [16]. (globaltechbiz.com)
- A high molecular weight isoform of this protein has also been shown to colocalize with Adaptor protein complex 2, beta-Adaptin and endodermal markers, suggesting an involvement in post-endocytic trafficking. (utsouthwestern.edu)
Endoderm4
- Endoderm development is an area of intense interest in developmental biology, but progress has been hampered by the lack of specific markers for differentiated endodermal cells. (xenbase.org)
- The endoderm -specific expression of Darmin makes this gene a useful marker for the study of endoderm development. (xenbase.org)
- Darmin is an endoderm -specific marker. (xenbase.org)
- The chemically induced endoderm expresses multiple endodermal markers, can participate in normal development when injected into developing embryos, and can form pancreatic progenitors. (harvard.edu)
Pancreatic1
- Analysis of pancreatic marker gene expression revealed that activation as well as repression of canonical Wnt signaling, early and late, inhibit exocrine pancreatic development. (uni-goettingen.de)
Embryonal carcinoma1
- It has many synonyms like endodermal sinus tumor, infantile embryonal carcinoma and orchid blastoma. (planetayurveda.net)
Pluripotency markers1
- Specifically, relative to 24-hr prestimulation, initiation of FOXD3 overexpression 48 hr prior to encapsulation yielded increased expression of pluripotency markers while reducing mesodermal but increasing endodermal germ layer marker expression. (trinity.edu)
Diagnosis4
- As stated in The BMJ 2009, tumor markers should not generally be used for the purpose of diagnosis of cancers, as opposed to monitoring purposes in certain cancers, or in certain cases, for screening purposes. (wikipedia.org)
- The various tumor markers differ in their usefulness for screening, diagnosis, prognosis, assessing therapeutic response, and detecting recurrence. (medscape.com)
- Because pathologic diagnosis of ovarian cancer is difficult without laparotomy, tumor markers such as CA-125, in addition to diagnostic imaging, are useful in preoperative evaluation for ovarian cancer. (medscape.com)
- A review of the literature in 1979 prior to the widespread use of combination chemotherapy found only 27% of 96 patients with stage I endodermal sinus tumor alive at 2 years after diagnosis. (kaiserpermanente.org)
Carcinomas1
- 60%). Nonseminomas include teratomas, embryonal carcinomas, endodermal sinus tumors (yolk sac tumors), and choriocarcinomas. (msdmanuals.com)
HESCs1
- Expression of osteogenic, chondrogenic, pluripotency, and germ layer markers by encapsulated hESCs was measured after 2 weeks of culture in osteogenic medium in the presence or absence doxycycline-induced FOXD3 transgene expression. (trinity.edu)
Derivatives1
- Endodermal cell derivatives, including lung, liver, and pancreas, are of interest for regenerative medicine, but efforts to produce these cells have been met with only modest success. (harvard.edu)
Cells8
- Tumor markers can be produced directly by the tumor or by non-tumor cells as a response to the presence of a tumor. (wikipedia.org)
- Importantly, E-PZ-iPS-like cells re-expressed basal epithelial cell markers (CD44, p63, MAO-A) in response to prostate-specific medium in spheroid culture. (nih.gov)
- Finally, when injected into mice, E-PZ-iPS-like cells expressed basal epithelial cell markers including CD44 and p63. (nih.gov)
- 1997). Within 14 days of postnatal development, Paneth cells mature and express cryptdin proteins as a specific marker (Bry, et al. (researchsquare.com)
- When the B7.6 blastomeres divide, they produce two distinct populations of daughter cells, two primordial germ cells (B8.12) and two endodermal strand cells (B8.11) [ 4 ]. (biomedcentral.com)
- One subset of postplasmic/PEM transcripts, still attached to the CAB, segregate into the endodermal strand cells (B8.11). (biomedcentral.com)
- Both the PGCs (B8.12 cells) and the endodermal strand cells (B8.11 cells) inherit these transcripts. (biomedcentral.com)
- CD16 and CD56 are markers for NK Cells *Rx for Mycoplasma pneumonia (cold agglutinins) mentioned in stem Erythromycin, Tetracycline *Guy had occult blood in stool and fibrosis in kidney Polyarteritis Nodosa *What type of HSN reaction is the PPD test for TB Type IV HSN Rx *ADH hormone 2nd messenger adenylyl cyclase / cAMP *Pt. (pdfcoffee.com)
Epithelial1
- Histological examination revealed three necessary features: the small-cell nature of the tumor, the presence of epithelial and neuroendocrine markers. (bvsalud.org)
Genes1
- In animal cap explants Fam132b is induced by Etv2/er71, which is known to activate expression of endothelial and hematopoietic genes in this system.Analysis of hematopoietic and vascular marker gene expression in Etv2/er71 expressing animal cap explants using multiplex Nanostring nCounter analysis revealed that Fam132b can enhance endothelial development at the expense of blood cell lineages. (uni-goettingen.de)
Teratoma1
- Example: elevated AFP in a child previously treated for teratoma suggests relapse with endodermal sinus tumor. (wikipedia.org)
Ovary2
- Endodermal sinus tumors of the ovary are particularly aggressive. (kaiserpermanente.org)
- Therapy of endodermal sinus tumor of the ovary. (kaiserpermanente.org)
Primitive1
- They are also known as endodermal sinus tumors or primitive endodermal tumors. (medscape.com)
Serum1
- Tumor markers can be determined in serum or rarely in urine or other body fluids, often by immunoassay, but other techniques such as enzyme activity determination are sometimes used. (wikipedia.org)
Biopsy1
- biopsy shows non-encapsulated, yellow, mucinous mass with endodermal sinuses with no evidence of metastatic disease. (rahulgladwin.com)
Ovarian2
- Newer markers for ovarian cancer are on the horizon. (medscape.com)
- Ovarian cancer, uterine cervical cancer, endometrial cancer, and trophoblastic neoplasms are gynecologic malignancies for which tumor markers are in clinical use. (medscape.com)
Lung1
- RNA targets include COVID-19 receptors and proteases, pulmonary alveolar type I and II markers, lung biology markers, viral response markers, and SARS-CoV-2 probes. (bioinnovations.in)
Cell1
- vas is a well-known germ cell marker. (biomedcentral.com)
Territories1
- When the chimeric proteins included activation domains, a rise in the NC territories tagged using the markers and was noticed. (globaltechbiz.com)
Immunohistochemistry1
- Immunohistochemistry methods were established and applied for the analysis of a selection of the chosen markers, following formal in-fixing and paraffinembedding. (dcu.ie)
Epithelium1
- At the beginning of intestinal development, proliferating progenitors that express ISC markers are ubiquitously distributed throughout the epithelium. (researchsquare.com)
Intestinal1
- Endodermal sinus tumor (rare subtypes are hepatoid and intestinal). (kaiserpermanente.org)
Specific1
- No marker is completely specific. (medscape.com)
Expression1
- IGF-1R could be included among con rmatory markers for ESTs and, from a therapeutic viewpoint, ESTs should also be examined for IGF-1R expression for bene cial regimens. (tubitak.gov.tr)
Recurrence1
- In the event of recurrence, confirmed residual disease, or elevated tumor markers after first-line chemotherapy, recommended treatment consists of paclitaxel (Taxol)-ifosfamide-cisplatin (TIP) or high-dose chemotherapy. (medscape.com)
Proteins1
- On the basis of their chemical nature, tumor markers can be proteins, conjugated proteins, peptides and carbohydrates. (wikipedia.org)
Early2
- Tumor markers are not elevated in all cancer patients, particularly patients with early-stage cancer. (medscape.com)
- In this study, we wanted to systematically analyze the role of the Wnt/β-Catenin signaling pathway during early and late phases of endodermal organogenesis. (uni-goettingen.de)
Clinical1
- No tumor markers with high sensitivity and high specificity for endometrial cancer are known at present, although CA-125 is often used in clinical practice. (medscape.com)
Patients1
- Tumor markers are soluble glycoproteins that are found in the blood, urine, or tissues of patients with certain types of cancer. (medscape.com)
Disease1
- There are many different tumor markers, each indicative of a particular disease process, and they are used in oncology to help detect the presence of cancer. (wikipedia.org)
Development1
- Many new tumor markers have been discovered since the development of monoclonal antibodies, and most tumor markers are now detected with them. (medscape.com)
Mutant1
- Conversely, whenever a dominating adverse mutant of and was indicated, a reduction in these NC markers was Azlocillin sodium salt reported. (globaltechbiz.com)