• Genes conferring neomycin-resistance are commonly included in DNA plasmids used to establish stable mammalian cell lines expressing cloned proteins in culture. (wikipedia.org)
  • Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells. (nature.com)
  • Multiplexed quantification of proteins and transcripts in single cells. (nature.com)
  • Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. (thermofisher.com)
  • Here, in order to discover those membrane proteins that may be involved in JEV attachment to or entry into virus permissive BHK-21 cells, a chemically mutated cell line (designated 3A10-3F) that became less susceptible to JEV infection was preliminarily established and selected by repeated low moi JEV challenges and RT-PCR detection for viral RNA E gene fragment. (biomedcentral.com)
  • Finally, two-dimensional electrophoresis (2-DE) coupled with LC-MS/MS was utilized to recognize the most differentially expressed proteins from membrane protein extracts of 3A10-3F and BHK-21 cells respectively. (biomedcentral.com)
  • The noted discrepancy of membrane proteins included calcium binding proteins (annexin A1, annexin A2), and voltage-dependent anion channels proteins (VDAC 1, VDAC 2), suggesting that these molecules may affect JEV attachment to and/or entry into BHK-21 cells and worthy of further investigation. (biomedcentral.com)
  • A recent paper also indicated that several proteins on the surface of C6/36 cells with masses ranging from 35-80 kDa and 150-200 kDa may bind to JEV, but failed to identify specific proteins by mass spectroscopic fingerprint analysis [ 11 ]. (biomedcentral.com)
  • The cloned genes encode R2R3 MYB proteins closely related to Antirrhinum AmMYBML2 and Petunia hybrida PhMYB1. (washington.edu)
  • We also identified homologous Darmin genes in other eukaryotes and in prokaryotes suggesting that Darmin is the founding member of a family of evolutionarily conserved proteins. (xenbase.org)
  • Furthermore, we found a distinctive type of retinal cells expressing the characteristic proteins of both bipolar cells and amacrine cells at P6, which may be an intermediate state in which amacrine cells were transforming into bipolar cells. (bvsalud.org)
  • Mesodermal lineage induction of PLA cells and clones resulted in the expression of multiple lineage-specific genes and proteins. (livingroom.health)
  • In addition to mesodermal capacity, PLA cells and clones differentiated into putative neurogenic cells, exhibiting a neuronal-like morphology and expressing several proteins consistent with the neuronal phenotype. (livingroom.health)
  • Stable cell lines expressing recombinant proteins are generated by experienced scientists working on protein engineering, expression, and purification, and cell line development services for decades. (welgen.com)
  • Stable cell lines developed with our proprietary protein expression system with multiple selectable markers is very productive in expressing target proteins. (welgen.com)
  • Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)
  • Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
  • DOCK4 and CEACAM21 as novel schizophrenia candidate genes in the Jewish population. (nih.gov)
  • This will be followed by combination of position cloning and sequencing candidate genes will be used. (energy.gov)
  • Another challenge to using transgenic plant technology in citrus is the long juvenile phase of immature tissues that ranges from 5 to 20 years, which greatly delays analysis of the effects of candidate genes. (springer.com)
  • Recent advances have been made in the of host factors, polymorphisms, and candidate genes associated characterization of the immune response to low-molecular- with occupational asthma may improve our understanding of weight agents. (cdc.gov)
  • Many commercially available protein expression plasmids contain a neo-resistance gene as a selectable marker. (wikipedia.org)
  • Our approach pairs sparse scATAC-seq data with robust detection of hundreds of cell surface and intracellular protein markers and optional capture of mitochondrial DNA for clonal tracking, capturing three distinct modalities in single cells. (nature.com)
  • ASAP-seq uses a bridging approach that repurposes antibody:oligonucleotide conjugates designed for existing technologies that pair protein measurements with single-cell RNA sequencing. (nature.com)
  • This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. (thermofisher.com)
  • Much earlier, a report stated that a 74 kDa protein on Vero cells was found to be capable of binding JEV and might be involved in virus uptake process [ 10 ]. (biomedcentral.com)
  • Expression vectors are designed for expressing the protein encoded by the inserted gene. (biologyexams4u.com)
  • Cosmid can be defined as the hybrid vectors derived from plasmids that contain cos site of phage which is essential for packaging of nucleic acid into protein coat plus essential features of plasmids and several unique restriction sites for the insertion of DNA to be cloned. (biologyexams4u.com)
  • By secretion cloning of Xenopus cleavage -stage embryos we isolated another novel protein, designated Darmin-related ( Darmin-r ) due to its sequence similarity with Darmin . (xenbase.org)
  • TMEM17 (transmembrane protein 173) is a 379 amino acid protein encoded by a gene mapping to human chromosome 5. (neobiotechnologies.com)
  • Theoretically, the possibilities include (1) a primary T-cell mediated autoimmune response causing muscle damage, (2) a primary degenerative process involving abnormal protein processing leading to a secondary inflammatory response, and (3) separate and independent immune and degenerative processes caused by an external trigger. (medscape.com)
  • Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
  • In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
  • Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
  • Deep sequencing followed by abundancy ranking pinpointed six top hit genes potentially associated with either improved or impaired endodermal differentiation, which were selected for functional validation in CRISPR-Cas9 mediated knockout (KO) lines. (mdpi.com)
  • N-myc downstream regulated gene 1 (NDRG1), also known as differentiation related gene 1, was previously identified as an up - regulated gene upon cellular differentiation. (scirp.org)
  • Even though its sequence along with its expressional pattern in cancer cell lines are evident, the functional aspects concerning cell proliferation, viability, differentiation and cell cycle regulation of NDRG1 remains vague. (scirp.org)
  • This resulted in over-expression of NDRG1, which in turn markedly up regulated two differentiation markers of the prostate tissue, p21 and cytokeratin 8/18. (scirp.org)
  • These results revealed that NDRG1 is functional in prostate cancer cells and able to induce expression of differentiation factors through p53 independent pathway. (scirp.org)
  • T. Napso, N. Azzam, A. Lerner and F. Fares, " N-myc Downstream Regulated Gene 1 Increases Differentiation Factors Level in Human Prostate Cancer Cells without Affecting Cell Proliferation and Cell Cycle Profiles," Journal of Cancer Therapy , Vol. 4 No. 2, 2013, pp. 568-574. (scirp.org)
  • A Novel Gene Which Is Up-Regulated during Colon Epithelial Cell Differentiation and Down-Regulated in Colorectal Neoplasms," Laboratory Investigation: A Journal of Technical Methods and Pathology, Vol. 77, No. 1, 1997, pp. 85-92. (scirp.org)
  • N. van Belzen, W. N. Dinjens, B. H. Eussen and F. T. Bosman, "Expression of Differentiation-Related Genes in Colorectal Cancer: Possible Implications for Prognosis," Histology and Histopathology, Vol. 13, No. 4, 1998, pp. 1233-1242. (scirp.org)
  • It is thought to play a role in T-cell proliferation and differentiation. (nih.gov)
  • A negative role for the interleukin-2-inducible T-cell kinase (ITK) in human Foxp3+ TREG differentiation. (nih.gov)
  • Special areas of interest include signal transduction and information processing, gene expression and gene regulatory networks, metabolism, proliferation, differentiation and morphogenesis, among others, and the use of multi-scale modeling to connect molecular and cellular systems to the level of organisms and populations. (iospress.com)
  • We have used RT-PCR to screen pluripotent murine embryonic stem cells to identify receptor tyrosine kinases (RTKs) potentially involved in the determination or differentiation of cell lineages during early mouse development. (silverchair.com)
  • In stratified epithelia proliferative basal cells adherent to the underlying basement membrane undergo cell cycle arrest then outward migration and terminal differentiation. (stanford.edu)
  • This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)
  • We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
  • The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)
  • Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
  • For over a century, mutations in the yellow gene, named for its effects on pigmentation, have been known to reduce male mating success. (elifesciences.org)
  • Geneticists have long suggested that mutations in insect pigment genes cause changes in the fly's brain because these pigments are made from dopamine, a chemical messenger that acts in the brain. (elifesciences.org)
  • Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. (thermofisher.com)
  • Nevertheless, NDRG1 sequencing assay disclosed no mutations in the gene. (scirp.org)
  • Downregulation of CDCA5 Can Inhibit Cell Proliferation, Migration, and Invasion, and Induce Apoptosis of Prostate Cancer Cells. (nih.gov)
  • Unpredictably, cell cycle progression, cell proliferation and DNA synthesis were unaffected fol lowing NDRG1 expression. (scirp.org)
  • Thymidine analog-retention assays revealed that Sox2 deficiency contributed to reduced survival of SCN neurons during the postnatal period of cell clearance, but did not affect progenitor cell proliferation or SCN specification. (bvsalud.org)
  • High transformation efficiency of citrus is of great importance for large scale characterization of gene functions and also cultivar development via transgenic and genome editing technologies. (springer.com)
  • Furthermore, human cDNA of NDRG1 from normal placenta was cloned into a eukaryotic expression vector and transfected into the three cancer cell lines. (scirp.org)
  • The 3 shRNA plasmids will then be co-transfected into 293 cells with an cDNA expression plasmid. (welgen.com)
  • We tried several gene selection strategies, and built classifiers using the resulting cDNA microarray gene lists. (lu.se)
  • Expected and potentially novel resistance genes are enriched in the top hits and are validated using individual sgRNA as well as cDNA overexpression. (cdc.gov)
  • However, it is difficult to capture the complexity of transcript isoform variance using these libraries, and large cDNA sequences are often difficult to clone into size-limited viral expression vectors. (cdc.gov)
  • Simply email us the sequence of your gene and we'll synthesize and clone it into any of our inducible vectors for little more than the cost of the empty vector. (systembio.com)
  • Any DNA molecule that has the ability to replicate inside the host to which the desired gene is integrated for cloning- include plasmids, bacteriophages, cosmids, BAC, yeast vectors, shuttle vectors etc. (biologyexams4u.com)
  • Cloning vectors are used for obtaining millions of copies of the cloned DNA fragments. (biologyexams4u.com)
  • The cloned genes in these vectors are not expected to express themselves at the transcription or translation level. (biologyexams4u.com)
  • Cloning vectors (Cloning vehicles) are used for creating genomic libraries, preparing probes, genetic engineering, or other basic studies. (biologyexams4u.com)
  • The most important requirement for recombinant DNA technology is the cloning vector and expression vectors. (biologyexams4u.com)
  • Cosmids are the novel cloning vectors which possess properties of both plasmid and phage. (biologyexams4u.com)
  • Choose from a number of promoter and marker combinations to express genes, miRNA, or shRNA. (systembio.com)
  • Three short hairpin RNA (shRNA) sequences will be selected and cloned into a pQuietU6 plasmid. (welgen.com)
  • Recent technological advances have enabled massively parallel chromatin profiling with scATAC-seq (single-cell assay for transposase accessible chromatin by sequencing). (nature.com)
  • The susceptibility to JEV of 3A10-3F cells was significantly weakened compared with parental BHK-21 cells, verified by indirect immunofluorescence assay, virus plague formation assay, and flow cytometry. (biomedcentral.com)
  • C1QBP knockdown and overexpression cells were established via lentiviral infection and subjected to apoptosis and ROS assay in vitro . (medsci.org)
  • Additional fee will be charged for Western blot or cell-based assay of your interest. (welgen.com)
  • Get inducible gene expression for mammalian systems with SBI. (systembio.com)
  • CHO suspension clonal stable cell lines are generated by electroporation using proprietary mammalian expression vector system with multiple selectable markers to express single gene of interest. (welgen.com)
  • Transfection into mammalian cells is followed by screening for cell lines permanently expressing specific genes through their incorporation into the cellular genome. (welgen.com)
  • Our experimental focus is on the mammalian setting using multiomics, informatics, mouse genetics, human genetics, single cell studies, and new human tissue platforms. (stanford.edu)
  • This gene encodes a member of the paired box family of transcription factors. (thermofisher.com)
  • The maize homeobox gene knotted1 ( kn1 ) encodes an AP2/ERF transcription factor (Hake et al. (springer.com)
  • This gene encodes an intracellular tyrosine kinase expressed in T-cells. (nih.gov)
  • The study reveals the importance of scientists considering that genes that affect behavior may do so by changing anatomy rather than by altering the brain. (elifesciences.org)
  • Loss of sensitivity to p21 cell cycle control may be associated with prostatic cancer behavior. (scirp.org)
  • Rowley had identified the first "translocation" in cancer, providing clear evidence that the cause of CML could be related to the fact that by moving from one chromosome to another, the aberrant segment of chromosome 22 was no longer sitting next to genes that controlled its behavior. (laskerfoundation.org)
  • All animals are composed of distinct body parts and organs, many of which are specified by particular combinations of `selector' genes that control cell and tissue behavior by regulating downstream `realisator' genes( Garcia-Bellido, 1975 ). (biologists.com)
  • uniExM may find many uses in the simple, multimodal nanoscale analysis of cells and tissues. (plos.org)
  • Nanoscale imaging enables the analysis of biological systems, such as cells and tissues, at the fundamental length scales of biomolecules and biomolecular interactions, but classically has required expensive equipment and advanced skillsets to perform, e . g ., via super-resolution microscopy [ 1 ]. (plos.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • fallopian tube and cells in lymphoid tissues. (neobiotechnologies.com)
  • Spearman analysis was employed for correlation analysis of genes in tumor tissues of RB patients. (bvsalud.org)
  • Skin malignancies, including epidermal squamous cell carcinoma (SCC), alone account for nearly as many cancers as all other tissues combined. (stanford.edu)
  • Nowell, a tumor biologist in the pathology department at the University of Pennsylvania School of Medicine, was interested in the relationship between cancer and alterations in genes (although he had no proof there was one). (laskerfoundation.org)
  • Nowell collaborated with the late David Hungerford who, he says, "knew more about chromosomes than I did," and together they made the startling observation that individuals suffering from chronic myelogenous leukemia (CML) had an abnormally small chromosome in the tumor cells. (laskerfoundation.org)
  • It is associated with Cockayne syndrome through the ERCC8 gene and familial adenomatous polyposis through the adenomatous polyposis coli (APC) tumor suppressor gene. (neobiotechnologies.com)
  • Interleukin-2 inducible T-cell kinase: a potential prognostic biomarker and tumor microenvironment remodeling indicator for hepatocellular carcinoma. (nih.gov)
  • One of the most convincing methods to verify a putative virus receptor is to transfer the receptor gene into a cell line that cannot bind virus and later demonstrate that the receptor-negative cell acquires the ability to bind virus and permit virus to replicate within it after the receptor gene is regained [ 13 ]. (biomedcentral.com)
  • Preliminary studies have recently identified a putative stem cell population within the adipose stromal compartment. (livingroom.health)
  • 11 ), and Vibrio splendidus, the progenitor of qnrS genes lone resistance ( 1 ). (cdc.gov)
  • Here, we report that the stem/progenitor cell transcription factor, Sex determining region Y-box 2 (Sox2), is required in the embryonic SCN to control the expression of SCN-enriched neuropeptides and transcription factors. (bvsalud.org)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • PURPOSE: To explore the mechanisms underlying stemness maintenance of retinoblastoma (RB) stem cells (RSCs). (bvsalud.org)
  • Much of the work conducted on adult stem cells has focused on mesenchymal stem cells (MSCs) found within the bone marrow stroma. (livingroom.health)
  • To confirm whether adipose tissue contains stem cells, the PLA population and multiple clonal isolates were analyzed using several molecular and biochemical approaches. (livingroom.health)
  • T. Okuda and H. Kondoh, "Identification of New Genes ndr2 and ndr3 Which Are Related to Ndr1/RTP/Drg1 but Show Distinct Tissue Specificity and Response to Nmyc," Biochemical and Biophysical Research Communications, Vol. 266, No. 1, 1999, pp. 208-215. (scirp.org)
  • Genetic engineering of T cells greatly increases the scope and potency of T-cell therapy: T-cell receptor (TCR) transfer allows targeting of intracellular cancer antigens, whereas chimeric antigen receptors (CARs) allow targeting of surface cancer or lineage-specific antigens. (ashpublications.org)
  • Overexpression in tobacco induces cell outgrowths in carpel epidermis and significantly increases the height of petal conical cells. (washington.edu)
  • The following product was used in this experiment: PAX8 (Renal Cell Marker) Recombinant Rabbit Monoclonal Antibody (ZR-1) from Thermo Fisher Scientific, catalog # 7849-RBM9-P1ABX. (thermofisher.com)
  • Loss of cell division cycle‑associated 5 promotes cell apoptosis by activating DNA damage response in clear cell renal cell carcinoma. (nih.gov)
  • However, the role of C1QBP in metabolism, oxidative stress, and apoptosis of renal cell carcinoma (RCC) cells have not yet been explored. (medsci.org)
  • Renal cell carcinoma is one of the most common cancers in the urinary system, causing over 140,000 deaths worldwide each year[ 1 ]. (medsci.org)
  • RQR8 is a 136-amino-acid epitope-based marker/suicide gene that enables clinical selection, cell tracking, and deletion in case of toxicity. (ashpublications.org)
  • A compact marker/suicide gene that utilizes established clinical-grade reagents and pharmaceuticals would be of considerable practical utility to T-cell cancer gene therapy. (ashpublications.org)
  • Our data have provided evidence for a comparable prediction of clinical outcome in CMF-treated breast cancer patients using conventional clinical variables and gene expression based markers. (lu.se)
  • [ 8 ] As such, tryptase is specific to mast cell granules and can provide information about mast cell number, distribution, and activation depending on the clinical context. (medscape.com)
  • The signature of our top screening hits is significantly correlated with gene expression data from clinical melanoma samples. (cdc.gov)
  • We found that the acidic domain of MZF-1 and the heparin-binding domain of Elk-1 facilitate the heterodimeric interaction between the two genes before the complex formation binds to the PKCα promoter. (oncotarget.com)
  • The effects of a maize knotted1 ( kn1) gene under the control of the cauliflower mosaic virus 35S promoter on genetic transformation efficiencies of six citrus genotypes were tested. (springer.com)
  • Achieving systematic, genome-scale perturbations within intact biological systems is important for elucidating gene function and epigenetic regulation. (cdc.gov)
  • YAC (Yeast Artificial Chromosome): A vector of hundreds to kilobases long used for cloning of DNA fragment. (biologyexams4u.com)
  • Marker genes enable measurement of transduction and allow selection of transduced cells, whereas suicide genes allow selective deletion of administered T cells in the face of toxicity. (ashpublications.org)
  • These subsequent effects are reversed by transfection with full-length PKCα, confirming that the MZF-1/Elk-1 heterodimer is a mediator of PKCα in TNBC cells. (oncotarget.com)
  • Endoderm development is an area of intense interest in developmental biology, but progress has been hampered by the lack of specific markers for differentiated endodermal cells. (xenbase.org)
  • We have shown that many Vibrionaceae species may resistance are chromosomally encoded, either a modifi ca- harbor chromosome-encoded qnr -type genes ( 13 ). (cdc.gov)
  • At a time when the idea that cancer had a genetic basis was widely disbelieved, Nowell's results provided the first clear evidence that a particular genetic defect in a single chromosome can lead to a population or clone of identical cells that accumulate in numbers to form a deadly malignancy. (laskerfoundation.org)
  • The tiny Philadelphia chromosome became a clear and consistent marker of CML, a cancer of the myeloid or bone marrow cells, with broad implications for diagnosis and prognosis of disease. (laskerfoundation.org)
  • I came to realize that there were many questions about chromosome changes in patients that would be rewarding to study," noted Rowley, and for the next decade she labored over the microscope looking at chromosomes in leukemic cells. (laskerfoundation.org)
  • Treacher Collins syndrome is also chromosome 5 associated and is caused by insertions or deletions within the TCOF1 gene. (neobiotechnologies.com)
  • The human tryptase gene is encoded on chromosome 16 and codes for four isoenzymes: alpha, beta, gamma, delta, and epsilon (Table 1). (medscape.com)
  • With all six genotypes of citrus tested including Eureka lemon, a cultivar difficult to be transformed, our results demonstrate that the kn1 gene may provide an effective molecular tool to enhance genetic transformation efficiencies of various citrus varieties. (springer.com)
  • So that it features which are useful for molecular cloning experiments. (biologyexams4u.com)
  • Importantly, high magnitude T cell responses are generated after two vaccine doses, with the majority of the T cell response directed against epitopes that are conserved between the prototype isolate B and the VOC. (nature.com)
  • We have created a highly compact marker/suicide gene for T cells combining target epitopes from both CD34 and CD20 antigens (RQR8). (ashpublications.org)
  • Finally, PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression. (livingroom.health)
  • Neomycin resistance is conferred by either one of two kanamycin kinase genes. (wikipedia.org)
  • The qnrS2 genes were identifi ed from Aeromonas mosome-encoded quinolone resistance determinants that punctata subsp. (cdc.gov)
  • The qnrS2 gene confer additional resistance to fl uoroquinolones ( 7 ). (cdc.gov)
  • This identifi cation of plasmid-mediated QnrS (QnrS1 and QnrS2), have been identifi ed in entero- qnr genes outside Enterobacteriaceae underlines a possi- bacterial species, sharing 41% and 60% amino acid identity ble diffusion of those resistance determinants within gram- with QnrA, respectively ( 8 - 10 ). (cdc.gov)
  • We also synthesize a library consisting of 70,290 guides targeting all human RefSeq coding isoforms to screen for genes which, upon activation, confer resistance to a BRAF inhibitor. (cdc.gov)
  • Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non-small cell lung cancer cells via p53-p21 signaling pathway. (nih.gov)
  • It also induces these same cells to secrete the long-range morphogen Wingless(Wg), which drives vg expression in surrounding cells up to 25-30 cell diameters away. (biologists.com)
  • Further, the construct binds the widely used pharmaceutical antibody rituximab, resulting in selective deletion of transgene-expressing cells. (ashpublications.org)
  • The control of this transition from epithelial stem cell to differentiated corneocyte, which is abnormal in epidermal cancers, is not well understood. (stanford.edu)
  • This arrest is caused by an abnormal expression of genes, often as a result of chromosomal translocations. (medscape.com)
  • One day while "diddling around with leukemic cells in culture," and rinsing them with tap water, Nowell noted that cells were dividing. (laskerfoundation.org)
  • K. Kokame, H. Kato and T. Miyata, "Homocysteine-Respondent Genes in Vascular Endothelial Cells Identified by Differential Display Analysis. (scirp.org)
  • It can influence numerous functions of the cells involved in the regulation of immune response and hematopoiesis including macrophages, dendritic cells, T lymphocytes, B lymphocytes and endothelial cells. (justia.com)
  • Fig. 3: Dissection of native human hematopoiesis with multimodal cell state inference and mtDNA-based lineage tracing. (nature.com)
  • Fig. 6: Multiplexed CRISPR perturbations with ASAP-seq in primary human T cells. (nature.com)
  • The latter encompass human skin regenerated on immune deficient mice as well as organotypic constructs with epithelial and stromal cells embedded within architecturally faithful mesenchyma in vitro. (stanford.edu)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • However, the pathway downstream NDRG1, involving p21 and c8/18, regulating cell cycle progression and DNA synthesis is unfunctional. (scirp.org)
  • and regulation of cell cycle process. (nih.gov)
  • In addition to classical gene regulatory networks noted above, we have recently identified a central role for additional biologic mechanisms, namely gene regulation by chromatin regulators and by noncoding RNAs. (stanford.edu)
  • Studies published in In Silico Biology generally use theoretical models and computational analysis to gain quantitative insights into regulatory processes and networks, cell physiology and morphology, tissue dynamics and organ systems. (iospress.com)
  • Acute lymphoblastic leukemia (ALL) is a malignant (clonal) disease of the bone marrow in which early lymphoid precursors proliferate and replace the normal hematopoietic cells of the marrow. (medscape.com)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)
  • Transcription factors associated with the development of bipolar cells and amacrine cells also support those changes. (bvsalud.org)
  • Our work indicated that inhibition of Sox2 could change cell fate by affecting transcription factors in the development of bipolar cells and amacrine cells, may provide new directions for the study and treatment of retinal genetic diseases and retinal dysplasia. (bvsalud.org)
  • ALL may be distinguished from other malignant lymphoid disorders by the immunophenotype of the cells, which is similar to B- or T-precursor cells. (medscape.com)
  • The malignant cells of acute lymphoblastic leukemia (ALL) are lymphoid precursor cells (ie, lymphoblasts) that are arrested in an early stage of development. (medscape.com)
  • Such coupling reactions, which occur predominantly in grasses, significantly decrease cell wall degradability and thus work as a barrier against efficient utilization of cell walls as a source of biomass for bioenergy production. (energy.gov)
  • s-IBM is characterized by the presence of non-necrotic myofibers invaded by mononuclear inflammatory cells, which, as a pathologic phenomenon, is significantly more common than vacuolated, congophilic, and necrotic fibers. (medscape.com)
  • Our faster and more predictive screening techniques may significantly increase speed of stable cell line development while improving quality and uniformity. (welgen.com)
  • Nam, A. S., Chaligne, R. & Landau, D. A. Integrating genetic and non-genetic determinants of cancer evolution by single-cell multi-omics. (nature.com)
  • T cells, macrophages, and myeloid dendritic cells all have the potential to invade non-necrotic muscle fibers. (medscape.com)
  • 1. We will generate a highly mutagenized population of Brachypodium with EMS in order to introduce new genetic variation into our model grass Brachypodium distachyona to be used as a resource for identification of the genes involved in feruloylation. (energy.gov)
  • The identification of these genes in Brachypodium will serve as a handle for gene discovery in important biofuel crops, such as switchgrass. (energy.gov)
  • Should have one or more marker genes for identification ad isolation of subpopulation of bacteria containing vector. (biologyexams4u.com)
  • Our results demonstrate that expression of the kn1 gene enhances transformation efficiencies from 3 to 15 fold compared to a control vector, 3-11 fold relative to the highest transformation efficiencies reported for these citrus genotypes. (springer.com)
  • We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)
  • Marker genes enable detection and selection of T cells, whereas suicide genes enable selective destruction of T cells in case of toxicity. (ashpublications.org)
  • From the abstract: 'Multicancer early detection (MCED) blood tests can detect a cancer signal from circulating cell-free DNA (cfDNA). (cdc.gov)
  • Epigenetic control of gene expression lasts through multiple cell divisions without alterations in primary DNA sequence and can occur via mechanisms that include histone modification and DNA methylation. (stanford.edu)
  • Click cells to view annotations. (jax.org)
  • Cytotoxic lymphocytes-related gene ITK from a systematic CRISPR screen could predict prognosis of ovarian cancer patients with distant metastasis. (nih.gov)
  • Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. (cdc.gov)
  • Such broad tropism of JEV rationally suggests that most possibly there exists more than one cellular receptor responsible for virus binding and entry into susceptible cells above mentioned. (biomedcentral.com)
  • For this reason, the availability of a specific virus receptor-negative/-defective cell line is usually a prerequisite for virus receptor confirmation. (biomedcentral.com)
  • The Drosophila wing primordium is defined by expression of the selector gene vestigial ( vg ) in a discrete subpopulation of cells within the wing imaginal disc. (biologists.com)