• Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. (wikipedia.org)
  • Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells. (nature.com)
  • Multiplexed quantification of proteins and transcripts in single cells. (nature.com)
  • The differentiation of the cells could be arrested by heterologous oncoproteins including AML1/ETO, PML/RARalpha, PLZF/RARalpha, Nup98/HoxA9, and other Hox proteins. (nih.gov)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • Translation factors control the translation of all proteins including those vital for cell growth and differentiation as well as those involved in cellular response to chemical toxicity. (cdc.gov)
  • Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)
  • However, previous fluorescent reporters for CM studies relied on the integration of fluorescent proteins into early CM marker like NKX2-5 or pan-CM marker like NCX1 . (biomedcentral.com)
  • Evidence is accumulating that small G proteins are involved in the regulation of cell growth and differentiation. (nii.ac.jp)
  • Among these regulatory proteins, we have cloned the cDNAs of smg p21 GDS, smg p25A GDI, and rho GDI, and made monoclonal antibodies against them. (nii.ac.jp)
  • Publications] Kaibuchi,K.: 'Molecular cloning and characterization of the stimulatory GDP/GTP exchange protein(GDS)for smg p21s,ras p21ーlike small GTPーbinding proteins. (nii.ac.jp)
  • These porous microstructures allow cell infiltration into the matrix and have fewer heterogeneous cellular proteins, thereby preventing complications, such as inflammatory reactions, fibrosis, calcification, and graft shrinkage. (biomedcentral.com)
  • C1QBP knockdown and overexpression cells were established via lentiviral infection and subjected to apoptosis and ROS assay in vitro . (medsci.org)
  • The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
  • Tumorigenic ALDHhigh/ABCB1negative cells generated nontumorigenic ALDHlow/ABCB1positive daughter cells in vitro and in tumor xenografts. (knaw.nl)
  • FACS derived iPSC lines express common markers of pluripotency, and possess spontaneous differentiation potential in vitro and in vivo. (columbia.edu)
  • Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
  • Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
  • Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
  • To explore whether the downregulation of AP-2alpha and AP-2gamma is of functional significance for lactogenic differentiation, we analyzed the expression of the AP-2 family members during the lactogenic differentiation of HC11 mammary epithelial cells in vitro. (biomedcentral.com)
  • Using RT-PCR we could not detect a downregulation of any of these genes during the lactogenic differentiation of HC11 cells in vitro. (biomedcentral.com)
  • The extent of lactogenic differentiation of HC11 cells in vitro is limited compared to mammary epithelium undergoing secretory differentiation in vivo. (biomedcentral.com)
  • To these ends, an in vitro system of lactogenic differentiation would be informative. (biomedcentral.com)
  • In a drop of blood we can identify all major blood cells and characterize their pathological changes in several disease conditions in vitro and in patient samples. (elifesciences.org)
  • This study explores the use of a simple human neurosphere-based in vitro model to characterise the pharmacological and toxicological effects of LiCl and VPA using gene expression changes linked to phenotypic alterations in cells. (plos.org)
  • Human embryonic stem cells (hESc) can also be used to analyze the in vitro development from undifferentiated pluripotent cells leading to terminally differentiated cell types, recapitulating the process of early embryonic development [5] . (plos.org)
  • In vitro analysis demonstrated that gremlin 1 partially inhibits Caco-2 cell differentiation upon confluence and activates Wnt signaling in normal rat intestinal epithelial cells. (utmb.edu)
  • Lgr5 resides in Wnt receptor complexes and mediates signaling of the Wnt-agonistic R-spondins (31), explaining the unique dependence of Lgr5 stem cells on secreted R-spondins in vivo and in vitro. (hubrecht.eu)
  • In this context, if it is possible to immortalize normal human SAE BC that retain the capacity to differentiate to ciliated, secretory, and other differentiated cell types in vitro on air-liquid interface (ALI) culture, it would be very useful to the investigation of SAE biology in health and disease, and the assessment of pharmacologic agents targeted to modify dysregulated BC biology relevant to the pathogenesis of human lung disease. (biomedcentral.com)
  • Identifying causal variants and genes from human genetic studies of hematopoietic traits is important to enumerate basic regulatory mechanisms underlying these traits, and could ultimately augment translational efforts to generate platelets and/or red blood cells in vitro. (biomedcentral.com)
  • Cost-effective blood cell generation will require novel strategies based on better knowledge of underlying mechanisms driving in vitro development. (biomedcentral.com)
  • Estrogens are important regulators of growth and differentiation in (range, 0 -9 fmol/mg protein, median 0.7). (lu.se)
  • However, the role of C1QBP in metabolism, oxidative stress, and apoptosis of renal cell carcinoma (RCC) cells have not yet been explored. (medsci.org)
  • C1QBP promotes the catabolism of hypoxanthine and elevates the apoptosis of RCC cells by modulating XDH-mediated ROS generation. (medsci.org)
  • STAT3 protein belongs to a group of intracellular transcription factors that mediate a variety of functions such as cellular differentiation, proliferation, and apoptosis. (biolegend.com)
  • Pathway analysis revealed the differential expression of genes involved in cell cycle maintenance and apoptosis, as well as genes in bone morphogenetic protein (BMP), Notch, Wnt, EPH, and MYC signaling pathways. (utmb.edu)
  • May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. (lu.se)
  • This is the first application of transcriptomics to assess ES cell derivatives and their in vivo counterparts -- a 'differentiomics' outlook. (news-medical.net)
  • The discovery of this new class of SSCs is based on the conjunction of evidence from unbiased single-cell molecular profiling and functional dissection of the BMSC lineage hierarchy using in vivo cell lineage analysis. (nature.com)
  • To investigate whether p27(Xic1) is necessary for cell cycle exit and/or neuronal differentiation, we used antisense morpholino oligos (MO) to knockdown the protein levels in vivo. (xenbase.org)
  • Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
  • MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
  • Downregulation of AP-2 transcription factor genes is not required for lactogenic differentiation of HC11 cells but may functionally be involved in aspects of lactogenic differentiation in vivo that are not reflected by the HC11 system. (biomedcentral.com)
  • To identify genetic programs of human colonic epithelial cell differentiation in vivo as well as candidate marker genes that define colonic epithelial stem/progenitor cells and the stem cell niche, we applied gene expression analysis of normal human colon tops and basal crypts by using expression microarrays with 30,000 genes. (utmb.edu)
  • A Novel Gene Which Is Up-Regulated during Colon Epithelial Cell Differentiation and Down-Regulated in Colorectal Neoplasms," Laboratory Investigation: A Journal of Technical Methods and Pathology, Vol. 77, No. 1, 1997, pp. 85-92. (scirp.org)
  • Collectively, the expression data set provides a comprehensive picture of human colonic epithelial cell differentiation. (utmb.edu)
  • Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
  • Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
  • While human induced pluripotent stem cells (hiPSCs) provide novel prospects for disease-modeling, the high phenotypic variability seen across different lines demands usage of large hiPSC cohorts to decipher the impact of individual genetic variants. (frontiersin.org)
  • The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
  • Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
  • Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) provide a valuable cell source for regenerative therapy, disease modeling, and drug screening. (biomedcentral.com)
  • Functional CMs derived from human pluripotent stem cells (hPSCs) would provide an unprecedented cell source for disease modeling, drug discovery, and cell transplantation therapy for CVD patients [ 3 ]. (biomedcentral.com)
  • A ) Morphology of Nrl -GFP wild-type (WT) and rd16 retinal organoids differentiated from mouse-induced pluripotent stem cells (iPSC) at various differentiation time points. (elifesciences.org)
  • Phenotypes of retinal organoids differentiated from induced pluripotent stem cells of Nrl -GFP rd16 mice. (elifesciences.org)
  • B ) Morphology of induced pluripotent stem cells (iPSC) colonies, ( C ) proliferation rate of iPSCs, and ( D ) photoreceptor primary cilium of organoids were compared between WT and rd16 . (elifesciences.org)
  • CRISPR/Cas9-mediated TPM1 knockout in human induced pluripotent stem cells (iPSCs) enhanced hematopoietic progenitor development, increasing total megakaryocyte and erythroid cell yields. (biomedcentral.com)
  • To validate this putative candidate gene and to explore its function, we used CRISPR/Cas9 genome editing to discover that cultured TPM1 -deficient induced pluripotent stem cells enhanced hematopoietic progenitor cell formation. (biomedcentral.com)
  • The molecular pathways of normal myeloid differentiation, as well as the mechanisms by which oncogenes disrupt this process, remain poorly understood. (nih.gov)
  • These myeloid cell lines provide tools for examining the biochemical and genetic pathways that accompany normal differentiation as well as a system in which to dissect how other leukemic oncoproteins interfere with these pathways. (nih.gov)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • We discuss the rationale of various anti-fibrogenic treatment strategies targeting the clonal hematopoietic stem/progenitor cell, aberrant signaling pathways, fibrogenic cytokines, and the tumor microenvironment. (haematologica.org)
  • Human colonic epithelial cell renewal, proliferation, and differentiation are stringently controlled by numerous regulatory pathways. (utmb.edu)
  • In addition, cluster of differentiation 24 (CD24)-negative NP responder clones formed spheroid structures in various culture systems, suggesting the preservation of a more immature phenotype compared to CD24-positive nonresponder clones. (biomedcentral.com)
  • The CD (cluster of differentiation) markers are frequently used in differential diagnosis of leukemia. (origene.com)
  • Molecular Biology of the Cell. (wikipedia.org)
  • Schier, A. F. Single-cell biology: beyond the sum of its parts. (nature.com)
  • Our cell lines yield novel insights into NP biology and provide promising new tools for studies of IVD development, cell function and disease. (biomedcentral.com)
  • In mammals, physiological Wnt signaling is intimately involved with the biology of adult stem cells and self-renewing tissues (18,19). (hubrecht.eu)
  • We were the first to link Wnt signaling with adult stem cell biology, when we showed that TCF4 gene disruption leads to the abolition of crypts of the small intestine (8), and that TCF1 gene knockout severely disables the stem cell compartment of the thymus (2). (hubrecht.eu)
  • To facilitate the study of the biology of the human SAE in health and disease, we immortalized and characterized a normal human SAE basal cell line. (biomedcentral.com)
  • The immortalized hSABCi-NS1.1 cell line has diverse differentiation capacities and retains SAE features, which will be useful for understanding the biology of SAE, the pathogenesis of SAE-related diseases, and testing new pharmacologic agents. (biomedcentral.com)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • Furthermore, the study examined the effects of cooperating oncoproteins such as Ras or Bcr/Abl, which allowed for both factor-independent proliferation and differentiation, or Bcl-2, which permitted factor-independent survival but not proliferation. (nih.gov)
  • Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
  • Depletion of the cell-cycle inhibitor p27 ( Xic1 ) impairs neuronal differentiation and increases the number of ElrC (+) progenitor cells in Xenopus tropicalis. (xenbase.org)
  • Depletion of p27(Xic1) in X. tropicalis caused an increase in proliferation and a suppression of the neuronal differentiation marker, N-tubulin. (xenbase.org)
  • Effect of p27Xic1 MO on neuronal differentiation. (xenbase.org)
  • We have previously utilised an NT2.D1 neurosphere based model of neuronal differentiation to study the effects of a variety of compounds on neural development [7] . (plos.org)
  • Human embryonic stem cells are so exciting because they provide a pathway of making many desperately-needed cell types for use in medicine," said Michael D. West, President & C.E.O. at ACT and an author on the paper. (news-medical.net)
  • In the ongoing debate over embryonic stem cells some have argued that the cells have not demonstrated their potential value in medicine. (news-medical.net)
  • Besides use in the aging retina, human embryonic stem cells have now demonstrated the potential to change the face of several age-related degenerative diseases, perhaps one day allowing physicians to prescribe young cells they way they dispense pills today. (news-medical.net)
  • In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
  • Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Furthermore, human cDNA of NDRG1 from normal placenta was cloned into a eukaryotic expression vector and transfected into the three cancer cell lines. (scirp.org)
  • This resulted in over-expression of NDRG1, which in turn markedly up regulated two differentiation markers of the prostate tissue, p21 and cytokeratin 8/18. (scirp.org)
  • Unpredictably, cell cycle progression, cell proliferation and DNA synthesis were unaffected fol lowing NDRG1 expression. (scirp.org)
  • These results revealed that NDRG1 is functional in prostate cancer cells and able to induce expression of differentiation factors through p53 independent pathway. (scirp.org)
  • N. van Belzen, W. N. Dinjens, B. H. Eussen and F. T. Bosman, "Expression of Differentiation-Related Genes in Colorectal Cancer: Possible Implications for Prognosis," Histology and Histopathology, Vol. 13, No. 4, 1998, pp. 1233-1242. (scirp.org)
  • Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
  • Differentiation was normal and terminal as evidenced by morphology, cell surface markers, gene expression, and functional assays. (nih.gov)
  • At the same time, we found an increase in the expression of ElrC, a marker of cells as they undergo a transition from proliferation to differentiation. (xenbase.org)
  • We report the isolation of a p27(Xic1) genomic clone and we identify a 5' region capable of driving reporter gene expression specifically in the neural tube and the eye. (xenbase.org)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
  • Differential cell surface marker expression and divergent responses to differentiation conditions suggest that the NP subtypes may correspond to distinct maturation stages and represent distinct NP cell subpopulations. (biomedcentral.com)
  • The relationship between drug resistance and tumor-initiating capacity was tested by fluorescence-activated cell sorting of colonosphere cells, based on expression of ABCB1 and aldehyde dehydrogenase (ALDH) activity. (knaw.nl)
  • Alterations in the expression of translation factors as molecular markers of carcinogenesis and chemical toxicity. (cdc.gov)
  • Further studies have been performed in our laboratory to determine whether alterations in the expression of various translation (initiation, elongation, and termination) factors can be used as molecular markers for carcinogenesis and chemical toxicity. (cdc.gov)
  • By employing the real time PCR technique, we have investigated the expression levels of various translation factors in human cancer cell lines and their corresponding controls. (cdc.gov)
  • Similarly, expression levels of the translation factors were investigated in human prostate epithelial cells, RWPE1, exposed to a cytotoxic concentration of cadmium chloride. (cdc.gov)
  • Among the translation factors studied, the expression level of translation elongation factor 1A2 (TEF1A2) exhibited the highest alteration (approximately 2000-fold overexpression) in the cancer cell lines compared with the corresponding controls. (cdc.gov)
  • These results thus demonstrate that translation factors may be cellular targets for carcinogenesis and chemical toxicity, and that alterations in their expression levels may be used as molecular marker for carcinogenesis and chemical toxicity. (cdc.gov)
  • The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
  • Secretory epithelial cells display lactogenic differentiation which is characterized by the expression of milk protein genes, such as beta-casein or whey acidic protein (WAP). (biomedcentral.com)
  • Differentiating HC11 cells displayed lower expression levels of milk protein genes than mammary glands of mid-pregnant or lactating mice. (biomedcentral.com)
  • The lactogenic differentiation of the secretory epithelial cells is characterized by the synthesis of lactose and milk fat and by the expression of milk protein genes, such as beta-casein or whey acidic protein (WAP). (biomedcentral.com)
  • In this report we analyzed AP-2 transcription factor expression in HC11 cells subjected to lactogenic differentiation conditions. (biomedcentral.com)
  • In the short-term AN model, iPS-MSCs conferred a substantial anti-apoptotic effect on tubular cells, associated with a downregulation of Bax and Bax/Bcl2 ratio and an upregulation of survivin expression. (oncotarget.com)
  • In addition, in order to phenotypically anchor the gene expression data, changes in the heterogeneity of cell subtype populations and cell cycle phase were monitored using flow cytometry. (plos.org)
  • Detection of changes in CD marker expression in leukemia is critical for understand the primary mechanisms initiating these changes. (origene.com)
  • Lentivirus-mediated expression of SPDEF in hSABCi-NS1.1 cells induced secretory cell metaplasia, accompanied with characteristic COPD-associated SAE secretory cell changes, including up-regulation of MSMB, CEACAM5 and down-regulation of LTF. (biomedcentral.com)
  • Microarrays may be used to assay gene expression within a single sample or to compare gene expression in 2 different cell types or tissue samples, such as in healthy versus diseased tissue. (medscape.com)
  • Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
  • Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)
  • OriGene offers a range of tools to analyze CD markers including cDNA clones, antibodies, overexpression cell lysates and more. (origene.com)
  • Nine hundred and sixty-nine cDNA clones were found to be differentially expressed between human colon crypts and tops. (utmb.edu)
  • Although bone marrow fibrosis is seen in a variety of malignant and non-malignant disease states, the deposition of reticulin and collagen fibrosis in the bone marrow of patients with myelofibrosis is believed to be mediated by the myelofibrosis hematopoietic stem/progenitor cell, contributing to an impaired microenvironment favoring malignant over normal hematopoiesis. (haematologica.org)
  • The bone marrow contains various populations of skeletal stem cells (SSCs) in the stromal compartment, which are important regulators of bone formation. (nature.com)
  • It is well-described that leptin receptor (LepR) + perivascular stromal cells provide a major source of bone-forming osteoblasts in adult and aged bone marrow. (nature.com)
  • These Fgfr3 + stem/stromal cells with OCT identities are abundant in the young bone marrow and depleted in the old bone marrow, denoting their transitional nature. (nature.com)
  • The term "transitional" emphasizes the unique feature of these cells that are particularly abundant in the young bone marrow. (nature.com)
  • However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
  • Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
  • The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
  • Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)
  • Hematopoietic stem cell transplantation remains the only curative therapeutic approach that reliably results in resolution of bone marrow fibrosis in patients with myelofibrosis. (haematologica.org)
  • Diagnosis typically requires demonstration of M-protein (sometimes present in urine and not serum but rarely absent entirely) and/or light-chain proteinuria, and excessive plasma cells in the bone marrow. (msdmanuals.com)
  • N-myc downstream regulated gene 1 (NDRG1), also known as differentiation related gene 1, was previously identified as an up - regulated gene upon cellular differentiation. (scirp.org)
  • Detailed understanding of the ontogenetic, cellular and molecular characteristics of functional intervertebral disc (IVD) cell populations is pivotal to the successful development of cell replacement therapies and IVD regeneration. (biomedcentral.com)
  • Profiling of a set of novel NP markers ( CD24 , CA12 , PAX1 , PTN , FOXF1 and KRT19 mRNA) in a representative set of subclones substantiated successful immortalization of multiple cellular subpopulations from primary isolates and confirmed their NP origin and/or phenotype. (biomedcentral.com)
  • Here we report the generation of clonal NP cell lines from nondegenerate human IVD tissue and present a detailed characterization of NP cellular subpopulations. (biomedcentral.com)
  • Successful development of cell replacement therapies and IVD regeneration is crucially dependent on an in-depth understanding of cellular and molecular characteristics of the functional IVD. (biomedcentral.com)
  • Transcriptional profiling and biochemical analysis of human lung cells exposed at the air-liquid-interface determined DNA damage and stress response, as well as perturbation of cellular metabolism, as major key events. (bvsalud.org)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • The aim was to identify cellular and cytogenetic markers of prognostic relevance to survival of high-risk MDS cases. (who.int)
  • Deep sequencing followed by abundancy ranking pinpointed six top hit genes potentially associated with either improved or impaired endodermal differentiation, which were selected for functional validation in CRISPR-Cas9 mediated knockout (KO) lines. (mdpi.com)
  • K. Kokame, H. Kato and T. Miyata, "Homocysteine-Respondent Genes in Vascular Endothelial Cells Identified by Differential Display Analysis. (scirp.org)
  • In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
  • The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
  • In the present study, we tested TALEN and double-nicking CRISPR/Cas9 on GS cells, targeting Rosa26 and Stra8 loci as representative genes dispensable and indispensable in spermatogenesis, respectively. (cyberleninka.org)
  • In fact, this was shown to be possible by the transfection of genes into GS cells (Kanatsu-Shinohara et al. (cyberleninka.org)
  • Amongst the intestinal Wnt target genes (13), we found the Gpr49/Lgr5 gene to be unique in that it marks small cycling cells at crypt bottoms. (hubrecht.eu)
  • Two other Wnt target genes, RNF43 and ZNRF3, encode stem cell-specific E3 ligases that downregulate Wnt receptors in a negative feedback loop (35). (hubrecht.eu)
  • Compared to the large airway cell line BCi-NS1.1, differentiated of hSABCi-NS1.1 cells on ALI were enriched with small airway epithelial genes, including surfactant protein genes, LTF and small airway development relevant transcription factors NKX2-1, GATA6, SOX9, HOPX, ID2 and ETV5. (biomedcentral.com)
  • To identify putative causal genes from these data, we performed computational modeling using available genome-wide association datasets for platelet and red blood cell traits. (biomedcentral.com)
  • Cadmium -induced cell transformation and tumorigenesis were found, at least in part, to be mediated through overexpression of TIF3 and TEF-1delta. (cdc.gov)
  • Translation initiation factor 5A2 (TIF5A2) and translation elongation factor 1delta (TEF1 delta) also exhibited significant overexpression (10- and 5-fold overexpression, respectively) in the tumor cells compared with the corresponding control cells. (cdc.gov)
  • Exposure of RWPE1 cells to a cytotoxic concentration of cadmium chloride also resulted in overexpression of several translation factors. (cdc.gov)
  • Transcription factors AP-2alpha and AP-2gamma are downregulated during lactation, and their overexpression in transgenic mice impaired the secretory differentiation of the mammary epithelium, resulting in lactation failure. (biomedcentral.com)
  • These professional antigen-presenting cells are able to discriminate unmodified self from non-self thanks to pattern recognition receptors such as the Toll like Receptors (TLR) and Scavenger Receptors (SR). SR were originally identified by their ability to bind and internalize modified lipoproteins and microorganisms but also particles and TLR agonists. (biomedcentral.com)
  • DC are professional antigen-presenting cells that are essential for initiating adaptive immune responses. (biomedcentral.com)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • Antibody- and T-cell-based approaches to targeting of B-cell maturation antigen have shown efficacy. (msdmanuals.com)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
  • We were able to identify two predominant clonal NP subtypes based on their morphological characteristics and their ability to induce SOX9 and COL2A1 under conventional differentiation conditions. (biomedcentral.com)
  • For induction of differentiation, cells were grown to confluency and then kept in medium without EGF for 48 hours to induce competence. (biomedcentral.com)
  • Even though its sequence along with its expressional pattern in cancer cell lines are evident, the functional aspects concerning cell proliferation, viability, differentiation and cell cycle regulation of NDRG1 remains vague. (scirp.org)
  • Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
  • This approach takes previous results of mechanical studies on specifically isolated blood cells to the level of application directly in blood and adds a functional dimension to conventional blood analysis. (elifesciences.org)
  • The stem cell-like cells of tumors are defined by their tumor-initiating capacity and may have retained these resistance mechanisms, making them resistant to chemotherapy. (knaw.nl)
  • However, ABCB1 was expressed only by a subpopulation of differentiated tumor cells that did not form clones or tumors. (knaw.nl)
  • CONCLUSIONS: The resistance of colorectal tumors to irinotecan requires the cooperative action of tumor-initiating ALDHhigh/ABCB1negative cells and their differentiated, drug-expelling, ALDHlow/ABCB1positive daughter cells. (knaw.nl)
  • These results suggest that smg p21 and smg p25A can be used as potential tumor markers for tumors derived from hematopoietic and neural tissues, respectively. (nii.ac.jp)
  • Fig. 3: Dissection of native human hematopoiesis with multimodal cell state inference and mtDNA-based lineage tracing. (nature.com)
  • Marking of quiescent LGR5+ CRC cells The observation a percentage of LGR5+ cell in lineage\tracing tests created few progeny may reveal a quiescent condition. (brinda.info)
  • Stem cell and differentiation markers were analyzed by immunoblotting and fluorescence-activated cell sorting. (knaw.nl)
  • Here, we describe an improved method for the derivation of iPSC lines using fluorescence activated cell sorting (FACS) to isolate single cells expressing the cell surface marker signature CD13NEGSSEA4POSTra-1-60POS on day 7-10 after infection. (columbia.edu)
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
  • Further, Fgfr3 + endosteal stromal cells give rise to aggressive osteosarcoma-like lesions upon loss of p53 tumor suppressor through unregulated self-renewal and aberrant osteogenic fates. (nature.com)
  • Clone- and tumor-initiating capacities were assessed by single-cell cloning and in immune-deficient mice. (knaw.nl)
  • Inhibition of the drug-efflux pump ABCB1 by PSC-833 allowed irinotecan to eradicate tumor-initiating cells. (knaw.nl)
  • Conversely, tumor-initiating cells were ABCB1-negative and were identified by high ALDH activity. (knaw.nl)
  • These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
  • tumor initiation capability of just one 1,000 and 200 sorted cells from PDO#7\LGR5\EGFP#1\produced subcutaneous xenografts. (brinda.info)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
  • It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial and neural cells. (bdbiosciences.com)
  • The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
  • Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)
  • ASAP-seq uses a bridging approach that repurposes antibody:oligonucleotide conjugates designed for existing technologies that pair protein measurements with single-cell RNA sequencing. (nature.com)
  • The cells were then stained with FITC Rat Anti-Mouse CD4 antibody (Cat. (bdbiosciences.com)
  • Right Panel: Mouse splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon APC-R700 Rat IgG1, λ Isotype Control (dashed line histogram) or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody (solid line histogram). (bdbiosciences.com)
  • antibody (clone AD2.35) on mouse primary astrocytes. (biolegend.com)
  • The cells were fixed, permeabilized, blocked and then stained with primary antibody, and Alexa Fluor® 594 conjugated secondary IgG (Cat. (biolegend.com)
  • The MI15 monoclonal antibody specifically binds to CD138 (Syndecan-1), an 85-92 kDa single chain transmembrane protein, which is strongly expressed on multiple-myeloma-derived cell lines and malignant plasma cell populations. (bdbiosciences.com)
  • We have also found that smg p25A is mainly detected inneural tissues by use of its specific monoclonal antibody, and that the levels of smg p25A mRNA increase in PC12 cells after their differentiation into neuron-like cells. (nii.ac.jp)
  • Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. (novusbio.com)
  • Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). (novusbio.com)
  • Western Blot: NOD2 Antibody (2D9) [NB100-524] - Whole cell protein from THP-1 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. (novusbio.com)
  • Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). (novusbio.com)
  • Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). (novusbio.com)
  • C ) Fluorescent images of dissociated day ( D ) 28 WT and rd16 cells stained by 4',6-diamidino-2-phenylindole (DAPI) and anti-GFP antibody. (elifesciences.org)
  • The activity of transcription factor IIIC1 is impaired during differentiation of F9 cells. (nih.gov)
  • In 1991, we reported the cloning of a T cell specific transcription factor that we termed TCF1 (1). (hubrecht.eu)
  • The Wnt target gene encoding the transcription factor Achaete scute-like 2 controls intestinal stem cell state (26). (hubrecht.eu)
  • Our approach pairs sparse scATAC-seq data with robust detection of hundreds of cell surface and intracellular protein markers and optional capture of mitochondrial DNA for clonal tracking, capturing three distinct modalities in single cells. (nature.com)
  • This report describes clonal, granulocyte-macrophage colony-stimulating factor-dependent myeloid cell lines that exhibit these properties. (nih.gov)
  • In this study, we aimed to investigate whether phenotypically distinct clonal cell lines representing different subpopulations in the human NP could be generated using immortalization strategies. (biomedcentral.com)
  • Long-term clonal culturing of organoids from Lgr5 stem cells. (hubrecht.eu)
  • One-way ANOVA was performed to compare the %GFP+ cells and viability of organoids from four different cell lines. (elifesciences.org)
  • Transcription factors have an important role in the ability of a cell to self-renew and also differentiate into most cell types, also known as pluripotency 1 . (biolegend.com)
  • They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
  • RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. (biolegend.com)
  • A major limitation in approaching this problem has been the lack of suitable cell lines that exhibit normal, terminal, and synchronous differentiation in the absence of endogenous oncoproteins and in response to physiologic cytokines, and whose differentiation can be arrested by ectopically expressed human oncoproteins. (nih.gov)
  • These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
  • Lesions are caused by bone replacement by expanding plasmacytomas or by cytokines that are secreted by malignant plasma cells that activate osteoclasts and suppress osteoblasts, leading to bone loss. (msdmanuals.com)
  • This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. (wikipedia.org)
  • The cell lines were established by conditional immortalization of primary murine marrow progenitors with an estrogen-regulated E2a/Pbx1-estrogen receptor fusion protein. (nih.gov)
  • Rb , or retinoblastoma protein, is a key regulator of the cell cycle, particularly during the transition from the G1 to S phases. (biolegend.com)
  • Within the brain, the protein is involved in the development of specialized cells that process smell. (biolegend.com)
  • Here, we established a hPSC line integrated with a mCherry fluorescent protein driven by the alpha myosin heavy chain (aMHC) promoter, which could be used to purify CMs based on the aMHC promoter activity in these cells. (biomedcentral.com)
  • Oxidative stress was markedly attenuated by iPS-MSCs both in AN mice and in protein-overloaded tubular cells. (oncotarget.com)
  • The resulting cell line (hSABCi-NS1.1) was characterized by RNAseq, TaqMan PCR, protein immunofluorescence, differentiation capacity on an air-liquid interface (ALI) culture, transepithelial electrical resistance (TEER), airway region-associated features and response to genetic modification with SPDEF. (biomedcentral.com)
  • When cultured on ALI, hSABCi-NS1.1 cells consistently formed tight junctions and differentiated into ciliated, club (SCGB1A1 + ), mucous (MUC5AC + , MUC5B + ), neuroendocrine (CHGA + ), ionocyte (FOXI1 + ) and surfactant protein positive cells (SFTPA + , SFTPB + , SFTPD + ), observations confirmed by RNAseq and TaqMan PCR. (biomedcentral.com)
  • In 15 to 20% of patients, plasma cells secrete only Bence Jones protein. (msdmanuals.com)
  • DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • their evolution from distinct cell lineages. (lu.se)
  • A first routine measure to narrow down diagnosis in clinical practice is the differential blood count, determining the frequency of all major blood cells. (elifesciences.org)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • BACKGROUND & AIMS: Stem cells of normal tissues have resistance mechanisms that allow them to survive genotoxic insults. (knaw.nl)
  • These epithelial organoid cultures are genetically and phenotypically extremely stable, allowing transplantation of the cultured offspring of a single stem cell, as well as disease modeling by growing organoids directly from diseased patient tissues (32, 47, 53). (hubrecht.eu)
  • Tissues and Cells. (lu.se)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • Stem cells are unique due to their ability to limitlessly self-renew and differentiate into each cell type in the adult body. (biolegend.com)
  • reported a protocol to selectively differentiate hPSCs into CMs with nodal-type characteristics, which relies on co-culture with visceral endoderm-like cells and subsequent culture in a serum-enriched medium [ 12 ]. (biomedcentral.com)
  • Autologous USCs were obtained and characterized, and their capacity to differentiate into urothelial cells (UCs) and smooth muscle cells (SMCs) was tested. (biomedcentral.com)
  • We have found that the levels of smg p21 mRNA increase in hematopoietic leukemiae cells after their differentiation into hematopoietic cells. (nii.ac.jp)
  • Comparative mRNA appearance level by genuine\period qPCR in cells expressing specific degrees of EGFP isolated from PDO#7\LGR5\EGFP#1 and #2 knock\in organoids. (brinda.info)
  • Evaluation of xenografts 96?h after induction with tamoxifen revealed the looks of the TOM+ side inhabitants, which retained appearance of LGR5 mRNA (Fig?EV4B and C) helping tracing through the LGR5+ cell inhabitants. (brinda.info)
  • Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescent staining prior to acquisition on a flow cytometer. (bdbiosciences.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • With therapeutic cloning, or the creation of banks of stem cells generated using parthenogenesis (perhaps as few as 40-400 lines), a limitless supply of immune-matched cells could be generated for most of the population. (news-medical.net)
  • The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
  • While several lines of evidence suggest a protective role of T cells against disease associated with Dengue virus (DENV) infection, their potential contribution to immunopathology in the acute phase of DENV infection remains controversial, and it has been hypothesized that the more severe form of the disease (dengue hemorrhagic fever, DHF) is associated with altered T cell responses. (mdpi.com)
  • asthma via IgE- and Th2-cell-mediated responses are of a hypersensitivity response in the respiratory tract precipitated by particular concern. (cdc.gov)
  • High-dose melphalan followed by autologous peripheral blood stem cell transplantation may also be used. (msdmanuals.com)
  • Nam, A. S., Chaligne, R. & Landau, D. A. Integrating genetic and non-genetic determinants of cancer evolution by single-cell multi-omics. (nature.com)
  • TALEN and CRISPR/Cas9, when applied in GS cells, will be valuable tools in the study of spermatogenesis and for revealing the genetic mechanism of spermatogenic failure. (cyberleninka.org)
  • 2004). In order to make GS cells more widely applicable for the study of spermatogenesis at the genetic and molecular levels, it is desirable to be able to modify their genome without disturbing their spermato-genic ability. (cyberleninka.org)
  • The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
  • The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. (justia.com)
  • Current methods to derive induced pluripotent stem cell (iPSC) lines from human dermal fibroblasts by viral infection rely on expensive and lengthy protocols. (columbia.edu)