• Maximum likelihood techniques for the mapping and analysis of quantitative trait loci with the aid of genetic markers. (agri.gov.il)
  • A method is presented to estimate the biometric parameters of a quantitative trait locus linked to a genetic marker when both loci are segregating in the F-2 generation of a cross between two inbred lines. (agri.gov.il)
  • The method, which assumes underlying normal distributions, is a combination of maximum likelihood and moments methods and uses the statistics of the genetic marker genotype samples for the quantitative trait to estimate the recombination frequency between the two loci and the means and variances of the genotypes of the quantitative trait locus. (agri.gov.il)
  • Linkage distance between the two loci was 38 map units and the effect of the quantitative trait locus was 1.6 phenotypic standard deviation units. (agri.gov.il)
  • The method is not effective in distinguishing between complete and partial linkage in samples of only 500 individuals or for quantitative loci with effects less than a phenotypic standard deviation. (agri.gov.il)
  • Power of daughter and granddaughter designs for determining linkage between marker loci and quantitative trait loci in dairy cattle. (agri.gov.il)
  • These results show that the average size of the quantitative trait loci effect was 1.6 phenotypic standard deviations for different traits and families. (cambridge.org)
  • The aims of quantitative trait loci detection is the potential use of these molecular markers to increase accuracy in predicting the genetic merit of breeding and its implementation in animal breeding schemes through marker-assisted selection. (cambridge.org)
  • Breeding opportunities for crop improvement have been greatly expedited using molecular mapping program of DNA markers and quantitative trait loci (QTL). (usda.gov)
  • Breeding opportunities for crop improvement have been greatly enhanced by quantitative trait loci (QTL) mapping technology. (usda.gov)
  • Linkage studies have identified several quantitative trait loci (QTLs) for RD. The QTL on chromosome 6p21.3 has been independently replicated by several groups and spans a 16.4-Mb (13.8 cM) interval from D6S109 to D6S291. (nih.gov)
  • A high density genetic linkage map for all chromosomes is necessary for quantitative trait loci (QTL) analysis and efficient marker-assisted breeding. (usda.gov)
  • Twenty‐seven quantitative trait loci ( QTL ) were detected for manually collected traits associated with biomass production. (osti.gov)
  • Quantitative trait locus (QTL) mapping locates and estimates the effect of genetic loci that regulate quantitative traits. (jax.org)
  • Quantitative trait loci analyses (log of the odds=15) indicated association of the genetic factor within a few centiMorgan of the best evidence for Ahl [Johnson et al. (cdc.gov)
  • Construction and improvement of a genetic map for peanut (Arachis hypogaea L.) continues to be an important task in order to facilitate quantitative trait locus (QTL) analysis and the development of tools for marker-assisted breeding. (usda.gov)
  • This marker set will be applicable for evaluating the genetic structure for association mapping, DNA fingerprinting, and mounting linkage maps and will be a practical tool set for further genetics. (springer.com)
  • Recently developed molecular genetics methods allow identification of genotypes based on DNA markers specific to a genotype. (usda.gov)
  • The general aim of the course is that students on completion of the course should have developed a population genetic way of thinking and use this to plan and perform genetic analyses, including analyses of results from population genetics research. (lu.se)
  • The course consists of different components including inheritance analyses of cross breeding and pedigree-data, use and properties of genetic markers and sequence data, non-Mendellian inheritance and epigenetic phenomena, basic linkage analyses and mapping, quantitative genetics and analysis of complex properties, classical population genetics and evolution of genetic and reproductive systems. (lu.se)
  • Marker-trait associations were assessed by using QTDT (a general test of association for quantitative traits) and the family-based association test (FBAT), and haplotype analysis was performed by using FBAT and the GeneHunter Transmission/Disequilibrium Test TDT. (nih.gov)
  • Most of the traits of importance to the breeder are genetically complex and quantitative in nature. (cropscience.org.au)
  • Therefore, considerable interest has emerged in the use of alternative methods for the mapping of quantitative traits. (cropscience.org.au)
  • New methods of mapping offer a promising route to the identification of genetic markers for the genes of complex traits and provide a foundation for molecular enhanced breeding of maize. (cropscience.org.au)
  • In the past years, I have been involved in several studies that contrast levels of population differentiation in putatively neutral marker genes with the corresponding data for potentially adaptive phenotypic traits to examine whether past selection pressures have been diversifying, unifying, or too weak to have any influence on the phenotypic variation. (lu.se)
  • However, radiologic quantitative assessment of tendon vascularity is not yet well established [ 5 ]. (hindawi.com)
  • This study was to evaluate the efficacy of CRP as a quantitative analysis for objective assessment of efficacy of three NSAIDs in postoperative inflammation and pain control. (ijdr.in)
  • CRP test proved to be a useful indicator as a quantitative assessment tool for monitoring postsurgical inflammation and therapeutic effects of various anti-inflammatory drugs. (ijdr.in)
  • CRP test is a useful indicator for quantitative assessment for comparative evaluation of NSAIDs. (ijdr.in)
  • While that is mostly true for visual assessment, there are quantitative methods to measure variation in the metabolic activity in the brain, which in turn helps detect the occurrence of neurolymphomatosis. (researchgate.net)
  • however, plasma or serum is the fluid of choice for studies requiring a quantitative assessment of exposure. (cdc.gov)
  • The various markers that enable assessment of the progression of preneoplastic lesions to spindle cell carcinoma include the p16 protein, which halts the cell cycle and induces apoptosis by pRb-mediated phosphorylation of cyclin-dependent kinase 4 (CDK4). (bvsalud.org)
  • However, its impor- tance in quantitative risk assessment cannot be overemphasized. (cdc.gov)
  • Exposure to a potential fetal health hazard can be estimated through ecological assessment (e.g., testing the community water supply), questionnaires (e.g., classifying residents accord- ing to whether they drink bottled or tap water), or biological markers (e.g., testing for exposures to chemicals or solvents found in tap water). (cdc.gov)
  • Initial assessment of quantitative immunoglobulins is useful, because patients with lupus often have hypergammaglobulinemia and have a higher incidence of immunodeficiency, including immunoglobulin A deficiency and other antibody defects. (medscape.com)
  • In this study, we performed sib-pair linkage analyses with Haseman-Elston and DeFries-Fulker methods to define more accurately the QTL interval. (nih.gov)
  • Out of 12 genes in the linkage interval, ten clustered within ~680 kb and were selected for association analysis based on central nervous system expression and putative function. (nih.gov)
  • Marker associations were detected in five of the ten genes, results that were corroborated by our haplotype TDT analysis. (nih.gov)
  • The genetic basis of the changes associated with the establishment of AM has been addressed with two complementary approaches: (i) Forward genetic screens to identify genes required for symbiosis, and (ii) transcript profiling to identify AM-induced genes followed by reverse genetic analysis of their function by gene knockouts or gene silencing. (frontiersin.org)
  • XMeis3 knockdown inhibits primary neuron and neural crest marker expression, but does not inhibit expression levels of Sox and Zic family genes or upstream regulators of neural crest. (xenbase.org)
  • Based on linkage disequilibrium (LD)-based association mapping used to detect single nucleotide polymorphism (SNP) markers associated with MGR against C. ribicola , MGR in these seed families appears to be controlled by Cr4 or other R genes in very close proximity to Cr4 . (frontiersin.org)
  • We then examined the relative expression of several mitochondrial markers, including Ppargc1α, Tfam, and electron transport chain genes and ATP levels, in skeletal muscle samples. (biomedcentral.com)
  • All mitochondrial markers, including Ppargc1a, Tfam, Cpt2, and electron transport chain genes, were upregulated in T2DM group compared to controls with no significant changes in the ATP levels. (biomedcentral.com)
  • Pathway enrichment analysis was performed to identify potential target genes to be analyzed in the validation step in an independent cohort. (oncotarget.com)
  • In the past two decades, QTL analyses have identified well over 2000 QTL associated with such diseases, 1,2,3 and the genes underlying these QTL are being identified at a faster pace than ever. (jax.org)
  • Obviously, the few ``low-risk'' patients who highlighting the need for a prognostic classification based would have relapsed after loco-regional therapy alone an optimised, computer-aided analysis of many genes [5]. (lu.se)
  • Using QCANet, we were able to extract several quantitative criteria of embryogenesis from 11 early mouse embryos. (nature.com)
  • Cluster analyses were used to distinguish the two putative genotypes, the least affected with NIHL (+/Ahl) and most affected with PTS (Ahl/Ahl). (cdc.gov)
  • Candidates would be expected to identify or create their own datasets using real examples FIMI campaigns across Europe and apply standard quantitative and qualitative analysis techniques. (ucd.ie)
  • There is a preference for topics with an empirical and quantitative focus, but social science and qualitative projects will be considered. (ucd.ie)
  • It will use the most recent qualitative and quantitative information and disaggregated data. (who.int)
  • Procedures are provided for perturbation of cells using siRNA and reliable methodology for marker detection by indirect immunofluorescence in 96-well formats. (jove.com)
  • Methods: Retrospective analysis of CT performed at 24-168 h post cardiac arrest on clinical indication within the Target Temperature Management after out-of-hospital cardiac arrest-trial. (lu.se)
  • 1977. Methods of air sampling and analysis. (cdc.gov)
  • Explore sustainable food production and learn Exergy Analysis techniques in this online Master's course on Sustainable Food and Bioprocessing. (wur.nl)
  • In this online master's course learn about analytical techniques used for analysis of pesticides, toxins, additives, biofunctionals and markers to trace food. (wur.nl)
  • Finally, we showed that USP19 overexpression is a surrogate prognostic marker of distant relapse in patients with early breast cancer. (nature.com)
  • Conse- markers could improve prognostic assessments and help quently, one might speculate that resources could be more to better discriminate which subjects are likely to re- profitably spent for the promotion of quality assurance spond to the various tailored systemic treatments. (lu.se)
  • E. Biganzoli, P. Boracchi / European Journal of Cancer 40 (2004) 1803-1806 re-analysis of Eden and colleagues are suitable for Some of the claims for the medical benefits of genomics have developing new prognostic classifications and/or com- undoubtedly been exaggerated, particularly with respect to the time-scale required for them to come to fruition. (lu.se)
  • The present study evaluated the effect of hapten and prohapten exposure on cell surface markers expression (CD86, CD54 and CD40) in the human monocytic leukemia, THP-1, cell line. (cdc.gov)
  • Upregulation of these markers was not observed after exposure to skin irritants or prohaptens in the absence of exogenously added S9. (cdc.gov)
  • 0.05) in nearly all of the models evaluated and the linear relative rate model with a 10 year exposure lag seemed to give the best fit in the Poisson regression analysis. (bmj.com)
  • Few epidemiological studies of workers with occupational exposure to respirable crystalline silica have collected quantitative exposure data appropriate for an exposure-response analysis and often there were potentially confounding exposures. (bmj.com)
  • After radiation exposure, all four tissues expressed elevated p53 along with other markers of DNA and cellular damage as expected. (sciencedaily.com)
  • The concentrations of cotinine and hydroxycotinine in body fluids can be used as markers for active smoking and as indices for secondhand smoke (SHS) exposure. (cdc.gov)
  • In this example, a biological exposure marker (blood lead-level) was available for classifying mothers according to their exposure to lead. (cdc.gov)
  • We isolated their neural correlates by means of systematic and quantitative meta-analyses of imaging studies. (mpg.de)
  • Ectopic expression of the HoxD1 antimorph protein knockdown inhibits primary neuron and neural crest markers in addition to hindbrain markers. (xenbase.org)
  • Quantitative analysis of serum free light chains. (wikipedia.org)
  • Titers of 64 for IgG and 32 for IgM analysis, and a negative control was created by using an in patient serum specimens were considered evidence of irrelevant monoclonal mouse antibody. (cdc.gov)
  • For that reason, serum was chosen as the matrix for the National Health and Nutrition Examination Survey (NHANES) cotinine analyses. (cdc.gov)
  • Serum specimens are processed, stored, and shipped to the Division of Laboratory Sciences, National Center for Environmental Health, and Centers for Disease Control and Prevention, Atlanta, GA for analysis. (cdc.gov)
  • A linkage analysis was performed using a regression interval analysis. (cambridge.org)
  • Multivariable stepwise logistic regression analysis, including all variables, was next used in propensity score matching analysis. (bvsalud.org)
  • Differences in all-cause mortality were analysed using Kaplan-Meier and Cox regression analyses. (bvsalud.org)
  • Accurate estimates of the genetic parameters and linkage distance for populations of 2000 individuals simulated with a segregating codominant locus with an effect of 1.63 standard deviations linked to a genetic marker with .2 recombination were also derived by this method. (agri.gov.il)
  • Design Systematic review and meta-analysis. (bmj.com)
  • Perivascular heterogeneity in clinical samples, and associations with other tumor features and outcome, remain largely unknown.Here we report a novel method for digital quantitative analyses of vessel characteristics and PC, which was applied to two collections of human metastatic colorectal cancer (mCRC).Initial analyses identified marker-defined subsets of PC, including cells expressing PDGFR-β or α-SMA or both markers. (lu.se)
  • Perivascular PDGFR-α and -β remained independent factors for survival in multivariate analyses.Overall, our study identified host vasculature and oncogenic status as determinants of tumor perivascular features. (lu.se)
  • The novel methodology should be suitable for similar analyses in other tumor collections. (lu.se)
  • As an important tool for the quantitative analysis of confocal microscopic data sets from co-expression of fluorescent proteins, we developed a semi-automated image analysis pipeline that allows for precise spatio-temporal quantification of protein co-localization and of the dynamics of organelle association from movies. (frontiersin.org)
  • To acquire quantitative criteria of embryogenesis from time-series 3D microscopic images, image processing algorithms such as segmentation have been applied. (nature.com)
  • Two CAPS (cleaved amplified polymorphic sequence) markers were also included to differentiate ahFAD2A alleles and ahFAD2B alleles. (usda.gov)
  • A total of 324 markers were anchored on this integrated map with 21 linkage groups including 17 aligned to corresponding A-subgenome or B-subgenome (chromosomes A1 to A10, and B1 to B4, B7, B8, and B9). (usda.gov)
  • C-reactive protein (CRP) estimation for quantitative analysis to assess anti-inflammatory action of nonsteroidal anti-inflammatory drugs (NSAIDs) after surgery in maxillofacial surgery. (ijdr.in)
  • Candidates interested in focusing on this or a similar topic would possess strong technical, programming and quantitative data analysis skills. (ucd.ie)
  • The report provides a complete analysis of the market, including historical data on the market for RT-PCR and microarrays, instruments, and reagents. (kaloramainformation.com)
  • Recent analyses of accumulated data indicated that certain criteria from the 2020 CDC MIS-C case definition performed better than others in distinguishing MIS-C from other illnesses and that certain other criteria likely contributed to misclassification. (cdc.gov)
  • For data analysis we used descriptive and chi-square statistics. (bvsalud.org)
  • Most of these studies have included analyses of phenotypic characters that are likely to be the targets of selection in natural populations. (lu.se)
  • To provide greater confidence when inferring genetic effects of small population size, we have extended our analyses to experimental systems or natural populations with a known history of fragmentation. (lu.se)
  • First, we co-expressed fluorescently tagged VAPYRIN with a range of subcellular marker proteins, and secondly, we employed biochemical tools to identify interacting partner proteins of VAPYRIN. (frontiersin.org)
  • Quantitative analysis of contrast-enhanced ultrasound could be a useful method to evaluate angiogenesis in healing tendons. (hindawi.com)
  • The highly significant overexpression of mitochondrial markers may be in response to free fatty acid overload. (biomedcentral.com)
  • Almost all treatments ameliorate mitochondrial markers' overexpression. (biomedcentral.com)
  • Cells expressing stem cell markers, including GFAP, and proliferation markers are closely apposed to the laminin-containing extracellular matrix (ECM) surrounding vascular endothelial cells. (nih.gov)
  • With this method, the genetic parameters of a locus affecting plant height linked to an electrophoretic marker for esterase were accurately estimated from a sample of 1596 F-2 progeny of a cross between two species of Lycopersicon (tomato). (agri.gov.il)
  • This study was conducted to quantify the amount of apoptotic markers in human end-stage heart failure and to correlate the results to clinical parameters of heart failure. (tum.de)
  • SUMMARY: Analysis of the potential association between VDR genetic variants and parameters such as glycemia, body mass index, body fat, and lipid levels improves the current understanding of the pathogenesis of T2D, MetS, overweight, and obesity. (bvsalud.org)
  • FAMEs analysis is a rationalisation of the more complex lipids from which component fatty acids are derived. (edu.au)
  • The same process can also be used for quantitative analysis of individual fatty acids. (edu.au)
  • In contrast, recent studies have demonstrated that high fat diet (HFD) may intensify mitochondrial biogenesis -via Ppargc1α and Tfam as the main regulators of mitochondrial biogenesis -and oxidative metabolism markers by increasing fatty acid oxidative capacity to handle this situation [ 9 ]. (biomedcentral.com)
  • Explore quantitative analysis and modelling principles. (wur.nl)
  • We explore whether levels of brain injury markers in blood could predict the likelihood of HIE on CT. (lu.se)
  • This white paper outlines an approach developed to address these limitations: an integrated assay solution that is based on Incucyte ® Live-Cell Analysis, along with a novel pH-sensitive dye coupled antibody fragment (Incucyte ® FabFluor Antibody Labeling Reagents). (news-medical.net)
  • To analyse the occurrence of metabolic risk using a non-invasive marker in schoolchildren. (bvsalud.org)
  • The use of non-invasive markers for the diagnosis of metabolic risk indicated a high occurrence of it in schoolchildren, with the girls presenting a higher risk. (bvsalud.org)
  • Results: statistical analysis revealed no association between markers. (bvsalud.org)
  • Results from field surveys, genetic analyses, stress-manipulation experiments and comparative studies of population structure indicate that several selection pressures, operating at both vegetative and reproductive stages, contribute to the maintenance of these pollen-color dimorphisms. (lu.se)
  • Contrary to the results of marker-gene analyses, our results for rare, threatened plants in Sweden and other areas provide little evidence for loss of adaptive potential in ecologically relevant characters with a polygenic basis. (lu.se)
  • Our results also indicate that the structuring of quantitative genetic variation can be relatively insensitive to landscape fragmentation, at least over the timescales considered by most conservation biologists. (lu.se)
  • The authors ask whether disparate results of recent consensus conferences, which ``good old'' clinical markers, if optimally analysed, might have attempted, using simple criteria, to define ``low- have a similar discriminating power with regard to breast risk'' patients for whom adjuvant chemotherapy should cancer prognosis as the microarray gene expression pro- not be recommended. (lu.se)
  • 7.5 credits), or BIOR60 Genetic analysis 2 (7.5 credits). (lu.se)
  • An Introduction to Genetic Analysis, 12th ed. (lu.se)
  • A genome examination using 85 informative molecular markers was conducted. (cambridge.org)
  • This programme is for plant breeding professionals who want to improve their knowledge of using molecular markers in genetic research and plant breeding, understanding the metaboli. (wur.nl)
  • Introduction The research of Hatch (1) has greatly increased our understand- ing of the use and potential limitations of biological markers for adverse reproductive effects. (cdc.gov)
  • To date, such biological markers have not been widely available nor have they been widely used when they are available. (cdc.gov)
  • In the past, transla- progesterone receptor (PgR) expression, and all of the tional research focused on single biological markers following features: pT 6 2 cm, grade I, age P 35 years which could putatively discriminate patients' prognosis should be considered in this low-risk category, whereas or treatment response. (lu.se)
  • This chapter discusses the taxonomic and evolutionary studies of cotton, its economic importance in the world, QTL mapping analysis of world researchers, as well as practical examples of MAS technology for exploiting the cotton genetic diversity. (usda.gov)
  • However, with an adeq battery of necf expsre blmarkers, prospective studies of en- vironmental effects on pregnancy outcomes might be possible. (cdc.gov)
  • By the from these conventional markers to determine individual same token, within the moderate/high-risk groups, pa- prognosis [6]. (lu.se)
  • A total of 4576 simple sequence repeat (SSR) markers were used for screening polymorphisms. (usda.gov)
  • A total of 4576 simple sequence repeat (SSR) markers from three sources: published SSR markers, newly developed SSR markers from expressed sequence tags (EST) and from bacterial artificial chromosome (BAC) end-sequences were used for screening polymorphisms. (usda.gov)
  • Transferability of Cucumber Microsatellite Markers Used for Phylogenetic Analysis and Population Structure Study in Bottle Gourd (Lagenaria siceraria (Mol. (springer.com)
  • This study provides one of the first quantitative views of population genetic variation in bottle gourd. (springer.com)
  • This is an incredibly exciting study showing that basic science done in rigorous quantitative fashion can lead to new important clinical discoveries. (sciencedaily.com)
  • This study was conducted to characterize Alberta limber pine seed families for WPBR resistance and to develop reliable molecular tools for marker-assisted selection (MAS). (frontiersin.org)
  • This study contributes to the development of fundamental approaches for assessing embryogenesis on the basis of extracted quantitative criteria. (nature.com)
  • This is a descriptive study with a quantitative approach, with random sample of 174 schoolchildren (70 boys and 104 girls) from 10 state high schools in the city of Passo Fundo, Rio Grande do Sul, Brazil. (bvsalud.org)
  • In this regard, we aimed to investigate the effect of high fat induced T2DM on mitochondrial markers of skeletal muscle, and an herbal component along with endurance exercise, as probable treatments, in AGE-rich high-fat diet (AGEs-HFD) induced T2DM mice. (biomedcentral.com)
  • 1990. Cytogenetic analyses of mice exposed to dichloromethane. (cdc.gov)
  • These mice were genotyped for two D10Mit markers. (cdc.gov)
  • Presented is a flexible informatics workflow enabling multiplexed image-based analysis of fluorescently labeled cells. (jove.com)
  • As a result, it is hard to accurately acquire quantitative criteria with the existing heuristic image processing-based segmentation algorithms. (nature.com)
  • The workflow quantifies nuclear and cytoplasmic markers and computes marker translocation between these compartments. (jove.com)
  • On the use of the moments method of estimation to obtain approximate maximum likelihood estimates of linkage between a genetic marker and a quantitative locus. (agri.gov.il)
  • Analysis of image‐based estimates of PAI successfully identified all four QTL hot spots for biomass production. (osti.gov)
  • Several of the six single-nucleotide polymorphisms tested in the GAD1 region demonstrated significant association in both stages, and a combined analysis in all 1128 subjects indicated that they formed a common high-risk haplotype that was significantly over-represented in cases ( P =0.003) with effect size OR=1.23. (nature.com)
  • This marker, plus three others that formed significant haplotype combinations in stage 1, did not replicate their association with the phenotype in stage 2. (nature.com)
  • Proper analysis involves the signal from the internalized antibody being sufficiently isolated from that outside the cells. (news-medical.net)
  • A frequency analysis was done to examine the difference in observed versus expected prevalence (calculated using a contingency table assuming independence across cells) of co-occurring complications or comorbidities. (cdc.gov)
  • Out of 14 GAD2 markers screened in stage 1, only one met the threshold criteria for follow-up in stage 2. (nature.com)
  • The Southern Cross Analytical Research Services (SCARS) sits within the Deputy Vice Chancellor (Research) portfolio and provides commercial and research analytical services from two key teams Environmental Analysis Laboratory (EAL) and Analytical Research Laboratory (ARL). (edu.au)
  • Therefore, it is the most satisfactory single screening test for an acute phase reactant and a useful marker for the amount of tissue injury and inflammation. (ijdr.in)
  • We used quantitative image analysis to compare the pattern of inflammation of these such as Mediterranean spotted fever, North Asian tick eschars with those from Mediterranean spotted fever. (cdc.gov)
  • This area of research has lagged con- siderably behind the field of developing new effect markers, such as semen analysis and early pregnancy loss. (cdc.gov)