Map peptide protein peptide mapping. Medical search. Frequent questions

Probing the wells with antibody and washing away unbound antibodies quickly reveals those peptides to which the antibody has bound. (mimotopes.com)
In a typical example, a library of monoclonal antibodies to a small protein, thought to recognize linear epitopes, was screened using a PepSet of biotinylated peptides (Figure 2). (mimotopes.com)
The monoclonal antibodies fell into groups recognizing one of three linear sequences of the protein. (mimotopes.com)
Western blots of immunoprecipitated proteins were developed using 12F3 (A) or anti-TFII-I antibody (B). (A) Star (*) and circle (o) indicate heavy and light chains of antibodies. (cytoskeleton.com)

The portable size ZipChip Interface directly mounts onto select models of Thermo Scientific mass spectrometers, and creates a seamless CE/ESI-MS workflow that offers fast CE separation, nano-spray level sensitivity, and HRAM spectrometry for the characterization of intact proteins, antibody drug conjugates (ADCs), antibody subunits, peptides, and metabolites. (thermofisher.com)
Linear antibody epitopes are readily mapped with PepSets. (mimotopes.com)
Antibody epitope mapping by ELISA is simple to perform using biotinylated peptides because the peptides can be captured onto Streptavidin™ or NeutrAvidin™ coated plates through the strong noncovalent avidin-biotin binding. (mimotopes.com)
The set consisted of short overlapping peptides derived from the protein being investigated and the peptides were captured onto plate wells coated with streptavidin before completing an ELISA with each monoclonal antibody (Figure 1). (mimotopes.com)
Custom synthesized biotinylated PepSets for linear antibody mapping are designed by the research scientist with help from experienced Technical Consultants at Mimotopes, and are shipped either ready to redissolve and plate or as ready-to-use plates already coated with the peptide (Figure 2). (mimotopes.com)
Anti-SUMO-2/3 mouse monoclonal antibody was raised against full-length recombinant SUMO-2 protein (Uniprot: P61956) combined with a proprietary mix of peptides that include CQIRFRFDGQPINE. (cytoskeleton.com)
The antibody has been shown to recognize a wide range of SUMO-2/3-targeted proteins in HeLa cell lysate (Fig. 1B) and to detect sub-nanogram amounts of recombinant SUMO-2 (Fig. 1A). (cytoskeleton.com)
Epitope mapping has identified that the antibody recognizes a sequence/structure within the peptide CQIRFRFDGQPINE. (cytoskeleton.com)
The peptide sequence is conserved in mammals, birds, and amphibians, giving the antibody broad species reactivity. (cytoskeleton.com)

Combining spatial and abundance information yielded an unprecedented quantitative view of HeLa cell anatomy and organellar composition, at the protein level. (elifesciences.org)

Likewise, advances in mass spectrometry proteomics now permit highly sensitive and accurate studies of protein expression at the whole proteome-scale. (biorxiv.org)
The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)
ABSTRACT In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. (who.int)
The study shows the usefulness of proteomics in identifying proteins that may express differences between sensitive and resistant L. tropica isolates. (who.int)

The biological system is complex with many regulatory features such as DNA, mRNA, proteins, metabolites, and epigenetic features such as DNA methylation and histone post-translational modifications (PTMs). (rsc.org)

We have developed and applied Dynamic Organellar Maps, a proteomic method that allows global mapping of protein translocation events. (elifesciences.org)
The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. (researchgate.net)
However, most proteomic studies rely on consensus databases to match spectra to peptide and proteins sequences, and thus remain limited to the analysis of canonical protein sequences. (biorxiv.org)

Gradually, the C-peptide part of proinsulin has evolved from being viewed upon as a side product of insulin synthesis and secretion to being considered as a bioactive peptide with endocrine functions. (researchgate.net)

PepSets have several advantages over other screening methods such as noncleaved peptides and blots. (mimotopes.com)

PG2 integrates genome and transcriptome sequencing to incorporate protein variants containing amino acid substitutions, insertions, and deletions, as well as non-canonical reading frames, exons, and other variants caused by genomic and transcriptomic variation. (biorxiv.org)
Most commonly, this approach leverages high-resolution mass spectrometric measurements of proteolyzed proteins, combined with tandem peptide fragmentation to match observed spectra with those expected from their amino acid composition. (biorxiv.org)

Rodda, S.J. (2002) Peptide Libraries for T-Cell Epitope Screening and Characterization. (mimotopes.com)
Mass spectrometry is among the most powerful analytical techniques available for protein characterization. (kbibiopharma.com)
Our expert team brings decades of experience in protein and peptide characterization to provide the right data with the right insight at all stages of development. (kbibiopharma.com)
In support of biotherapeutics across the board, KBI's Mass Spectrometry Core Facility has championed the characterization and management of proteins, active pharmaceutical ingredients (APIs), and impurities through identification, characterization, assessment, and quantitation. (kbibiopharma.com)

Indanomycin is biosynthesized by a hybrid nonribosomal peptide synthase/polyketide synthase (NRPS/PKS) followed by a number of `tailoring' steps to form the two ring systems that are present in the mature product. (iucr.org)
Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. (who.int)

Current approaches for peptide and protein identification use advanced statistical and graphical methods to match mass spectra and estimate their confidence. (biorxiv.org)
The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)

When a bioactive sequence is already known, analoging studies can be carried out using the set of peptides comprising single-point substitution sequences, or a combination of 2 or more selected substitutions. (mimotopes.com)
New data indicate that proinsulin C-peptide, contrary to previous views, exerts important physiological effects and shows the characteristics of a bioactive peptide. (researchgate.net)

We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)

We now provide evidence that two growth-regulated, nucleus- and cytoplasm-localized protein kinases, 90-kDa ribosomal S6 kinase (RSK) and mitogen-activated protein kinase (MAP kinase), contribute to the serum-induced phosphorylation of c-Fos. (nih.gov)
The major phosphopeptides derived from biosynthetically labeled c-Fos correspond to phosphopeptides generated after phosphorylation of c-Fos in vitro with both RSK and MAP kinase. (nih.gov)
The phosphorylation sites identified for RSK (Ser-362) and MAP kinase (Ser-374) are in the transrepression domain. (nih.gov)
This study suggests a role for nuclear RSK and MAP kinase in modulating newly synthesized c-Fos phosphorylation and downstream signaling. (nih.gov)
A splicing mutation in the Sik3 protein kinase gene causes a profound decrease in total wake time, owing to an increase in inherent sleep need. (nature.com)

Analysis of the blue coral transcriptome revealed enrichment of genes involved in stress response, including heat-shock proteins and antioxidants, as well as genes participating in signal transduction and stimulus response. (nature.com)
Recent multi-omics studies have revealed the heterogeneity and complexity of tumor features such as their genetic mutations, transcriptome, proteins, and signaling pathways. (rsc.org)

Sequences exhibiting enhanced bioactivity, or inhibitory properties, are candidates for development as peptide or peptidomimetic drugs. (mimotopes.com)
11 And human diseases, including cancer in particular, tend to be defined by the presence of proteins with altered and pathogenic sequences. (biorxiv.org)
The matching is com- plicated by the fact that there are protein modifications and the sequence databases store the unmodified sequences. (lu.se)

However, recent studies in many organisms and in humans have revealed significant protein sequence variation due to the presence of somatically acquired genetic variants, alternative transcription, and mRNA splicing, which are not necessarily annotated in reference databases. (biorxiv.org)
The proposed method is novel because it works independently of protein sequence databases and without any prior knowledge about modifica- tions. (lu.se)
PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)

Sets of overlapping peptides from potential target vaccine proteins are screened with immune sera or cells to find vaccine or diagnostic test candidates. (mimotopes.com)

The obvious next step is to integrate this information and learn how genes, proteins, and/or epigenetic factors influence the phenotype of a disease in context of the system. (rsc.org)
In addition to the host regulatory mechanisms response to disease, the microbiome can make changes to the expression of the host features such as their genes, proteins, and/or PTMs. (rsc.org)
10 Similarly, in recent studies, at least one third of genes in diverse organisms can exhibit alternative transcription, leading to the production of N-terminally extended proteins or alternative reading frames. (biorxiv.org)

We initially used maps statically to generate a database with localization and absolute copy number information for over 8700 proteins from HeLa cells, approaching comprehensive coverage. (elifesciences.org)

Furthermore, we demonstrate that the cytoplasmic and recombinant forms of soluble Toc159 bind directly and selectively to the transit peptides of these representative photosynthetic preproteins, but not representative constitutively expressed plastid preproteins. (rupress.org)

Each cell contains ten or more organelles, and several thousand different types of proteins. (elifesciences.org)
The 1-3mg scale PepSet is enough peptide for several thousand ELISA tests. (mimotopes.com)

The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)

This application is summarized in a separate article entitled Truncated Peptide Libraries for Cytotoxic T-Cell Epitope Mapping, available on the Mimotopes website. (mimotopes.com)

Independent of these, its biophysical properties and peptide interactions point to still further roles of C-peptide, in particular regarding possible. (researchgate.net)

Dynamic Organellar Maps enable the proteome-wide analysis of physiological protein movements, without requiring any reagents specific to the investigated process, and will thus be widely applicable in cell biology. (elifesciences.org)

In this review, we discuss considerations of the study design for each data feature, the limitations in gene and protein abundance and their rate of expression, the current data integration methods, and microbiome influences on gene and protein expression. (rsc.org)

set out to develop a method that reveals the locations of all the proteins in a cell at any given time. (elifesciences.org)

Cooperative phosphorylation at these sites by both enzymes was observed in vitro and reflected in vivo by the predominance of the peptide phosphorylated on both sites, as opposed to singly phosphorylated peptides. (nih.gov)
Natural products classed as polyketides, nonribosomal peptides and hybrids of both are often biosynthesized by giant, complex enzymes known as polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS). (iucr.org)

These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)

Consequently, a lot of effort is put into identifying the constituent proteins using mass spectrometric methods. (lu.se)

Moreover, since changing the location of a protein can change its activity, it is also important to be able to detect changes in the location of proteins under different circumstances, such as before and after drug treatment. (elifesciences.org)

Proinsulin C-peptide is known to bind specifically to cell membranes and to exert intracellular effects, but whether it is internalized in target cells is unknown. (researchgate.net)

Proteins are the tools and machines of the cell, and each organelle has its own set of proteins that it requires to work correctly. (elifesciences.org)
The location of many proteins in a cell is unclear or simply not known. (elifesciences.org)
The resulting technique maps the location of most of the proteins in a human cancer cell line and, in addition, determines how many copies of each protein there are. (elifesciences.org)
The new mapping method could be used in the future to analyse the anatomy of different cell types, such as nerve cells and cells of the immune system. (elifesciences.org)
T-cell epitopes (Helper and Cytotoxic) are easily located using PepSets provided peptides of the correct length are used2. (mimotopes.com)
A University of California, Irvine-led team of researchers working at the Center for Neural Circuit Mapping find links between brain disorders and dysfunction of newly identified inhibitory brain cell types. (news-medical.net)
In this study, using confocal microscopy and immunostained or rhodamine-labeled peptide, we show that C-peptide is internalized and localized to the cytosol of Swiss 3T3 and HEK-293 cell. (researchgate.net)
Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)
protein patterns of subjects with leish- Cell culture maniasis [17]. (who.int)

The Mass Distance Fin- gerprint is calculated from high accuracy measured peptide masses. (lu.se)

For example, most human tissues in healthy individuals acquire somatic nucleotide substitutions, insertions, deletions and DNA rearrangements, leading to the production of variant protein isoforms. (biorxiv.org)

Zinc binding to the peptide replica and analogs to residues 93-115 of horse liver alcohol dehydrogenase (ADH) was examined by competition of the peptides and the chromophoric chelator 4-(2- pyridylazo)resorcinol for zinc and X-ray absorption fine structure analysis of the zinc ligands. (researchgate.net)

This revealed which proteins had changed location, identifying these proteins as important for the cell's response to the growth factor. (elifesciences.org)

Subcellular localization critically influences protein function, and cells control protein localization to regulate biological processes. (elifesciences.org)

Our preliminary study using 2D isolates were recovered from liq- new cases throughout the world cu- gel electrophoresis in drug sensitive/ uid nitrogen, and sub-cultured in taneous leishmaniasis (CL) remains resistant strains of L. tropica showed RPMI1640 medium (Gibco/BRL) a serious public health problem in that some proteins were differentially supplemented with 10% fetal bovine numerous countries [1,2]. (who.int)

We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)

A team of UCI scientists led by Xiangmin Xu, PhD, professor of anatomy and neurobiology and director for the Center for Neural Circuit Mapping (CNCM) at the UCI School of Medicine, found that a distinct new class of brain inhibitory neurons when functioning abnormally may contribute to neurological and psychiatric conditions, including autism and schizophrenia. (news-medical.net)

Sample preparation (protein and drug-resistant L. tropica parasites extraction) niasis, early diagnosis and appropriate [8]. (who.int)

In order to regulate protein activity, many biological processes involve changes in protein subcellular localization. (elifesciences.org)

Anatomy14

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Chemicals and Drugs119

Analytical, Diagnostic and Therapeutic Techniques and Equipment50

Phenomena and Processes54

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