Mammary stem cells promotes mammary. Medical search. Frequent questions

The Notch signaling targeted luminal progenitor cells for expansion, which led to hyperplasia and tumorigenesis, the researchers report. (genengnews.com)
Recognition of the effective involvement of the myoepithelial cells in mammary gland development and in hampering tumorigenesis has renewed the interest in investigating the biological roles of this second main mammary lineage. (biomedcentral.com)
To determine whether NOTCH1 mediated mammary tumorigenesis is driven by a rare tumor initiating cell, we performed an in vivo limiting dilution assay. (hdac-inhibitors.com)
For the past 20 years, plenty of studies have suggested that only a small subpopulation of the cancer cells with tumor-initiating capability is the core origin of the tumorigenesis and the subset of cancer cells was named cancer stem cells (CSCs). (bmbreports.org)
ErbB3 ablation impairs PI3K/Akt-dependent mammary tumorigenesis. (ucdenver.edu)
We crossed the MMTV‐PyMT model of mammary gland tumorigenesis with a TgfbiΔ/Δ mouse and studied the CSC content of the tumours. (unifr.ch)

Glial cell line-derived neurotrophic factor family receptor alpha (GFRα) members have been widely connected to the mechanisms contributing to cell growth, differentiation, cell migration and tissue maturation. (medsci.org)
The glial cell line-derived neurotrophic factors (GDNFs), a family of neurotrophic factors, were initially thought to be able to regulate the growth, survival, and differentiation of neural-derived cell types. (medsci.org)
Based on whether it cooperates with the second receptor RET, GFRα has also been widely linked to the mechanisms that contribute to cell growth, differentiation and migration and tissue maturation. (medsci.org)
To date, RUNX2 has been involved in diverse physiological processes, including osteogenic differentiation of mesenchymal stem/stromal cells, chondrocyte hypertrophy, immunomodulation, vascular invasion and endothelial cell migration via modulating a variety of signaling cascades (e.g. (spandidos-publications.com)
A triple function has been accomplished: transport of drugs or biomolecules that help in the differentiation and proliferation of stem cells, cell targeting and contrast agents for real-time monitoring and obtaining bioimages for diagnostic and detection systems. (eurekaselect.com)
This discovery builds on the team's 2006 identification of mouse mammary stem cells (MaSCs) and progenitor subpopulations, which enhanced the prospect of investigating the genetic control of their lineage specification and differentiation. (genengnews.com)
Mesenchymal stem cells (MSCs) show trilineage differentiation because these cells differentiate into osteocytes, chondrocytes and adipocytes. (nature.com)
These tumors were distinct from mouse mammary tumor virus (MMTV)-neu tumors, and contained elevated transcripts for factors associated with luminal/alveolar expansion and differentiation, suggesting that they arose from physiologic targets of prolactin. (biomedcentral.com)
Mammalian adult stem cells resemble the blastomeres of planktonic and benthic organisms with small eggs and may have evolved in mature organisms as an adaptation to the growth and maintenance of tissues via proliferation and the regulation of organ size via cell loss (e.g., terminal differentiation). (iospress.com)
Furthermore, high glucose and other factors in DM could promote M-MDSC differentiation to M1 pro-inflammatory macrophage cells, thus inhibiting wound healing. (biomedcentral.com)
Inducing cancer cell differentiation would abolish their self-renewal and invasive capacity and could be combined with the current standard of care, especially in poorly differentiated and aggressive tumors (with worst prognosis). (bvsalud.org)
We demonstrate here that microRNA 203, a potent driver of differentiation in pluripotent stem cells (ESCs and iPSCs), promotes the differentiation of mammary gland tumor cells. (bvsalud.org)
Combining mouse in vivo approaches and both mouse and human-derived tridimensional organoid cultures, we report that miR-203 influences the self-renewal capacity, plasticity and differentiation potential of breast cancer cells and prevents tumor cell growth in vivo. (bvsalud.org)
Our work sheds light on differentiation-based antitumor therapies and offers miR-203 as a promising tool for directly confronting the tumor-maintaining and regeneration capability of cancer cells. (bvsalud.org)
CSCs take charge of a cancer hierarchy, harboring stem cell-like properties involving self-renewal and aberrant differentiation potential. (bmbreports.org)
A stem cell commences as an undifferentiated cell that can either undergo self-renewal, whereby it generates daughter cells that remain as stem cells, or mature into a specific cell type via differentiation (Can/Hematol 2008). (papersowl.com)
TGF-β affects development and differentiation of human natural killer cell subsets. (sunnybrook.ca)
HEB-deficient T-cell precursors lose T-cell potential and adopt an alternative pathway of differentiation. (sunnybrook.ca)
Scientists found that the cell viability and half-maximal inhibitory concentration of glioma stem cells (GSCs) against temozolomide significantly decreased after GSCs underwent serum-induced differentiation to adherent growth of tumor cells. (stemcellsciencenews.com)
Scientists propose a monophyletic origin of cancer model which suggests that CSCs are stem cells that lose control of differentiation. (stemcellsciencenews.com)
TGFβ-1 induced morphological and functional changes of cells are the result of substantial gene regulation and protein alterations leading to: loss of epithelial cell-cell adhesion and apical-basolateral polarity, change of differentiation markers, acquisition of fibroblastoid shape, reversion of intermediate filaments, gain of cell motility and increased extracellular proteolysis [ 3 ]. (biomedcentral.com)
Vascularization or vasculogenesis is defined as the differentiation of epithelial progenitor cells (EPCs) into mature epithelial cells to enable the formation of new blood vessels. (naturalnews.com)
In their previous study, the researchers found that epimedin C could induce endothelial-like (but not osteogenic) differentiation of C3H/10T1/2 cells in vitro. (naturalnews.com)
C3H/10T1/2 is a clonal mouse embryo line used in the developmental determination of vertebrate stem cell lineages and their subsequent differentiation. (naturalnews.com)
Based on these results, the researchers concluded that epimedin C effectively promotes vascularization both in the BMP2-dependent bone formation model and in the 4T1 mammary tumor-bearing model by inducing the endothelial-like differentiation of C3H/10T1/2 in vivo. (naturalnews.com)
2012 Nevertheless the in vivo systems that restrict epithelial lineage plasticity to guard differentiation and exactly how such systems control stem cell function during MG morphogenesis and regeneration stay poorly understood. (buyresearchchemicalss.net)

Identification of genomic alterations that drive the clinically aggressive serous and clear cell tumors of the endometrium. (researchgate.net)
Many tumors display intracellular heterogeneity, with subsets of cancer stem cells (CSC) that sustain tumor growth, recurrence, and therapy resistance. (mdc-berlin.de)
Here we investigated the interactions between CSCs and CAFs in mammary gland tumors driven by combined activation of Wnt/{beta}-catenin and Hgf/Met signaling in mouse mammary epithelial cells. (mdc-berlin.de)
We compared levels of transcripts in the ERα-associated "luminal" signature that defines this subtype of tumors in women and transcripts enriched in various mammary epithelial lineages to other well-studied genetically modified murine models of breast cancer. (biomedcentral.com)
They constituted a heterogeneous, but distinct group of murine mammary tumors, with molecular features of the luminal subtype of human breast cancer. (biomedcentral.com)
A hallmark of many malignant tumors is dedifferentiated (immature) cells bearing slight or no resemblance to the normal cells from which the cancer originated. (bvsalud.org)
Four independent NOTCH1 driven mouse mammary tumors were injected as serial dilutions into the thoracic mammary fat pad of immunodeficient mice, and recipient mice were monitored for disease development. (hdac-inhibitors.com)
Tumorsphere selleckchem INCB018424 forming cells increase after neoadjuvant chemotherapy, and the molecular profile of tumors obtained after chemotherapy resembles the gene expression profile of tumorsphere cells, suggesting that this assay enriches for tumor initiating cells. (hdac-inhibitors.com)
The tumorspheres appeared enriched in Notch1 active cells, as Deltex1 expression was increased in the spheres compared with the primary tumors from which they were derived. (hdac-inhibitors.com)
The mammary tumors predominantly express the luminal lineage mar ker keratin 8/18. (hdac-inhibitors.com)
Mutant PIK3CA accelerates HER2-driven transgenic mammary tumors and induces resistance to combinations of anti-HER2 therapies. (ucdenver.edu)

Mammary tissues were obtained from these mice according to established guidelines approved by the Institutional Animal Care and Use Committee at the University of Texas Health Science Center. (springeropen.com)
Furthermore, the percentage of basal cells characterized with EpCAM + CD49f hi , the fraction highly enriched with mammary stem cells, was significantly lower with the fast digestion method (0.5 ± 0.1 % of total cells, Mean ± SE, n = 3) when compared with the slow digestion protocol (8.0 ± 0.8 %, P = 0.016, paired t test) using mammary tissues from the same mice (Fig. 1 a). (springeropen.com)
Oncogenic mutations or abnormal expression of signaling components disrupt the regulatory networks that govern cell function, thus enabling tumor cells to undergo dysregulated mitogenesis, to resist apoptosis, and to promote invasion to neighboring tissues. (eurekaselect.com)
TP63 is a member of the tumour suppressor TP53 gene family, and ΔNp63α, a TP63 splicing variant, is constitutively expressed in the stem cell-containing basal layer of stratified epithelial tissues, including the mammary gland, where it plays a critical role in stemness and tissue development. (researchsquare.com)
The mammary gland is composed of numerous elongated ductal branches embedding in a background of fatty tissues. (biomedcentral.com)
Strategies for regenerative therapies in adult mammals, therefore, might be based on stimulating growth of adult stem cells or their surrogates in specific tissues rather than on introducing embryonic stem cells into adults. (iospress.com)
Our group combines computational and experimental techniques to study the cellular organization of complex tissues, with a focus on determining the phenotypic diversity and clinical significance of tumor cell subsets. (stanford.edu)
Totipotent stem cells contain all the constituents necessary to produce a living being, given that these cells can supply all embryonic and extraembryonic tissues required for proper growth (Can/Hematol 2008). (papersowl.com)
We currently have projects focusing on mast cells, that produce a wide array of mediators that can remodel tissues during immune responses and regulate tumour progression. (queensu.ca)
2009 whereas incomplete and reversible EMT takes place during morphogenesis of specific epithelial tissues such as for example mammary gland WYE-354 (MG) (Nakaya and Sheng 2013 Although very much has been learned all about the molecular systems that promote EMT during early advancement and in tumor cells hereditary pathways that regulate incomplete EMT during tissues morphogenesis to keep epithelial lineages are badly characterized. (buyresearchchemicalss.net)
Right here we offer in vivo proof to get a previously unrecognized system that defends epithelial identification during mammary tissues morphogenesis and regeneration that involves Ovo-like 2 (Ovol2) an associate from the Ovo category of zinc finger TFs that are recognized to regulate epithelial advancement in epidermis aswell as mammalian epidermis and testis (Dai et al. (buyresearchchemicalss.net)
We employ diverse cellular and molecular techniques and experimental models including gene arrays and single nuclear RNA sequencing for gene discovery, cell lines for analyses of signaling pathways, and human tissues and mouse models of diseases to address basic goals with translational potential. (uams.edu)

C-jun protects cells from UV-induced apoptosis, and it cooperates with NF-κB to prevent apoptosis induced by TNFα. (wikipedia.org)
This suggests that c-jun regulates cell cycle progression and apoptosis through two separated mechanisms. (wikipedia.org)
The cyclic change of the c-jun protein levels is significant in the proliferation and apoptosis of glandular epithelial cells. (wikipedia.org)
The persistent stromal expression of c-jun protein may prevent stromal cells from entering into apoptosis during the late secretory phase. (wikipedia.org)
The DNA damage response (DDR) is a mechanism that protects cells against radiation-induced oxidative DNA damage by causing cell cycle arrest and apoptosis. (researchsquare.com)
On the other hand, both the ectopic and entopic expression of ΔNp63α in apoptosis-sensitive hiPSCs reduced the expression levels of BAX after irradiation and significantly decreased the number of apoptotic cells induced by radiation. (researchsquare.com)
Ionizing radiation is well known to induce oxidative DNA damage, such as DNA double-strand breaks (DSBs), and consequently trigger the DNA damage response (DDR), including cell cycle arrest and apoptosis. (researchsquare.com)
To examine the potential action of ABGE against gastric cancer , the present study evaluated its effect on the inhibition of cell proliferation and induction of apoptosis in SGC-7901 human gastric cancer cells. (drcalapai.com)
Dual inhibition of Type I and Type III PI3 kinases increases tumor cell apoptosis in HER2+ breast cancers. (ucdenver.edu)
A successful therapy must therefore eliminate these cells known to be highly resistant to apoptosis. (hindawi.com)
In this paper, we describe the metabolic changes as well as the mechanisms of resistance to apoptosis occurring in cancer cells and cancer stem cells, underlying the connection between these two processes. (hindawi.com)
In addition to inducing necrosis and apoptosis, ROS induces autophagic cell death. (biomedcentral.com)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)
Mechanistically, an upregulation of PDL-1 in chemotherapy-treated CAFs led to an increase in hepatocyte growth factor (HGF) secretion, which stimulates cancer progression, cell invasion, and stemness of lung cancer cells, while inhibiting apoptosis. (cdc.gov)

Overexpression of c-jun in cells results in decreased level of p53 and p21, and exhibits accelerated cell proliferation. (wikipedia.org)
Transferred mitochondria accumulate reactive oxygen species, promoting proliferation. (utah.edu)
Their larval and adult cells have narrow potencies, sometimes coupled to virtually unlimited … proliferation, and function in the growth, maintenance and regulation of body size. (iospress.com)
The embryos of larger arthropods and deuterostomes with well-provisioned eggs or viviparity, on the other hand, exhibit regulative development, while their larval "set-aside" or adult stem cells function in the growth, maintenance, and regulation of organ size coupled to constrained proliferation and cell turnover. (iospress.com)
Mammalian embryonic stem cells would seem adapted to rapid proliferation, functioning in part to enclose yolk or to acquire access to maternal resources. (iospress.com)
Wound healing is a multifaceted and dynamic process which includes four overlapping phases of coagulation, inflammation, proliferation, and remodeling, which requires a well-orchestrated integration of the complex biological and molecular events of cells and cell signaling [ 5 ]. (biomedcentral.com)
MicroRNA miR-199a-3p regulates cell proliferation and survival by targeting caveolin-2. (sunnybrook.ca)
Cell proliferation involves the replication of all cellular contents with the required energy for this to happen. (hindawi.com)
To prevent aberrant cell proliferation, these pathways are tightly regulated. (hindawi.com)
This activation is necessary for both cell proliferation as well as glucose uptake and use. (hindawi.com)
Low levels of ROS can promote cell proliferation but high levels induce cell death [ 13 ]. (biomedcentral.com)

In contrast, the different fast digestion protocols, as described in published studies, yielded high percent of non-epithelial cells with very few basal epithelial cells liberated in our hands. (springeropen.com)
The three sets of markers tested in our hands reveal rather equally efficiency in separating luminal and basal cells if same fluorochrome conjugations were used. (springeropen.com)
However, the tendency of non-epithelial cell inclusion in the basal cell gate was highest in samples profiled by CD24/CD29 and lowest in samples profiled by CD49f/EpCAM, this is especially true in mammary cells isolated from C57BL/6J mice. (springeropen.com)
This finding will have significant implication when sorted basal cells are used for subsequent gene expression analysis. (springeropen.com)
Likewise, strategies for the containment of cancer might be based on promoting normal pathways of cell loss, the basal mode for handling excess cells. (iospress.com)
MG goes through dramatic tissue development and redecorating during puberty and being pregnant generating not merely luminal epithelial cells but also a distinctive mesenchymal-like epithelial inhabitants specifically basal/myoepithelial cells (Watson and Khaled 2008 Hence MG acts as a perfect system to review the hereditary circuits that control epithelial lineage plasticity. (buyresearchchemicalss.net)
The basal/myoepithelial inhabitants of adult MG provides the so-called multipotent mammary stem cells (MaSCs) that upon transplantation can handle regenerating a whole epithelial network made up of both luminal and basal/myoepithelial lineages (Shackleton et al. (buyresearchchemicalss.net)
2006 Adult stem cells with bipotential or unipotential are also within the mammary basal area via lineage tracing under physiological circumstances (Rios et al. (buyresearchchemicalss.net)
We have found that maternal metabolic perturbations (pre-pregnancy through lactation) in mouse models profoundly influence mammary tumor risk in progeny, that mammary adiposity significantly affects underlying mammary epithelial subpopulations with tumor-initiating potential, and that high-fat diet induced oxidative stress is associated with deregulated transcriptional profiles of oncogenes, tumor suppressors and inflammatory cytokines, leading to abnormal expansion of mammary epithelial basal stem-cell like populations. (uams.edu)
Overexpression of IRS-1 resulted in inhibition of basal autophagy, and reduced oxidative stress-induced autophagy and cell death. (biomedcentral.com)
Knockdown of autophagy-related gene 5 inhibited basal autophagy and diminished oxidative stress-induced autophagy and cell death. (biomedcentral.com)
Our results suggest that overexpression of IRS-1 promotes cells growth, inhibits basal autophagy, reduces oxidative stress-induced autophagy, and diminishes oxidative stress-mediated autophagy-dependent cell death. (biomedcentral.com)

This ana lysis revealed that the mammary tumor initiating cell in this mammary tumor model is relatively rare and that the bulk of the NOTCH1 transformed mammary tumor cells lack the capacity to initiate disease in immunodeficient recipient mice. (hdac-inhibitors.com)
Pri mary NOTCH1 transformed mammary tumor cells formed spheres in culture at a rate of 1 in 268. (hdac-inhibitors.com)

In this study, we compared two frequently used digestion methods and three sets of frequently used surface markers for their efficiency in enriching mammary stem and progenitor cells in two commonly used mouse strains, C57BL/6J and FVB. (springeropen.com)
CD24/CD29) for enriching mammary stem and progenitor cells. (springeropen.com)
To address some of these shared obstacles by new investigators in this field, we compared two frequently used digestion methods and three sets of frequently used surface markers for their efficiency in enriching mammary stem and progenitor cells in two commonly used mouse strains, C57BL/6J and FVB. (springeropen.com)
At puberty mammary epithelial stem/progenitor cells that have a home in the terminal end buds (TEBs) go through collective migration to operate a vehicle ductal morphogenesis (Ewald et al. (buyresearchchemicalss.net)
2001 These results imply both epithelial plasticity-promoting and -restricting systems might be very important to the morphogenic potential of TEB WYE-354 stem/progenitor cells (Godde et al. (buyresearchchemicalss.net)

About 80% of all newly diagnosed breast cancers have luminal cell phenotype and they express estrogen receptor (ERα). (nature.com)
Li Q, Cao Z, Zhao S. The Emerging Portrait of Glial Cell Line-derived Neurotrophic Factor Family Receptor Alpha (GFRα) in Cancers. (medsci.org)
Our studies demonstrate that prolactin can promote diverse carcinomas in mice, many of which resemble luminal breast cancers, providing a novel experimental model to examine the pathogenesis, progression and treatment responsiveness of this tumor subtype. (biomedcentral.com)
We also study contributions of F-BAR proteins in metastatic cancers using cell and mouse models. (queensu.ca)
Transforming growth factor beta-1 (TGFβ-1) is a strong inducer of type-3 EMT in mammary cancers [ 2 ]. (biomedcentral.com)
Furthermore compelled expression from the repressor isoform of Ovol2 can reprogram metastatic breasts cancers cells from a mesenchymal for an epithelial condition. (buyresearchchemicalss.net)

Dr. Welm completed her PhD in Cell and Molecular Biology at Baylor College of Medicine in Houston, TX under the supervision of Gretchen Darlington, PhD. She then went on to conduct postdoctoral training in Dr. J. Michael Bishop's laboratory at the University of California, San Francisco where her work focused on developing new models of breast cancer metastasis. (utah.edu)
Dr. Welm's group discovered that the Ron kinase pathway is an important facilitator of breast cancer metastasis through its unique dual function in tumor cells and in resident macrophages. (utah.edu)
Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. (bnwax.com)
Cancer stem cells, instrumental in metastasis, would seem to ignore mechanisms normally functioning in the removal of excess cells. (iospress.com)
English Breast cancer metastasis is a complex process that depends not only on intrinsic characteristics of metastatic stem cells, but also on the particular microenvironment that supports their growth and modulates the plasticity of the system. (unifr.ch)
In search for microenvironmental factors supporting cancer stem cell (CSC) growth and tumour progression to metastasis, we here investigated the role of the matricellular protein transforming growth factor beta induced (TGFBI) in breast cancer. (unifr.ch)
Versican G3 promotes mouse mammary tumor cell growth, migration, and metastasis by influencing EGF receptor signaling. (sunnybrook.ca)
My research program is investigating paracrine signaling pathways linked to mast cell activation, systemic mastocytosis and cancer metastasis. (queensu.ca)
49 Madison Black, Ivraym Barsoum, Peter Truesdell, Tiziana Cotechini, Margaret Petroff, Rob Siemens, Madhuri Koti, Andrew Craig and Charles Graham (2016 ) Activation of the PD-1/PD-L1 immune checkpoint confers chemoresistance and promotes tumor metastasis . (queensu.ca)

AKT signaling in normal and malignant cells. (eurekaselect.com)
Numerous vectors have been engineered for the sole purpose of killing cancer cells, and some have successfully suppressed malignant tumours. (weeksmd.com)
We hypothesized that cysteine cathepsins affect transforming growth factor β-1 (TGFβ-1)-induced EMT of normal and malignant mammary epithelial cells. (biomedcentral.com)
The role of lysosomal proteolysis in TGFβ-1-induced EMT and invasion was investigated in a normal and a novel malignant murine mammary epithelial cell line. (biomedcentral.com)
We found increased lysosome activity during EMT of malignant mammary epithelial cells. (biomedcentral.com)
2011 Latest generally in vitro research have implicated many EMT-inducing transcription elements (EMT-TFs) such as for example Snail Slug and Zeb1 as critical indicators that promote stemness in regular and malignant mammary epithelial cells (MECs) (Chaffer et al. (buyresearchchemicalss.net)

Stromal Gas6 promotes the progression of premalignant mammary cells. (machadolab.org)
Macrophages promote the progression of premalignant mammary lesions to invasive cancer. (machadolab.org)

Studies have shown that c-jun is required for progression through the G1 phase of the cell cycle, and c-jun null cells show increased G1 arrest. (wikipedia.org)
Therefore, c-jun is required for maintaining sufficient cyclin D1 kinase activity and allowing cell cycle progression. (wikipedia.org)
Those results indicate that c-jun downregulates p53 to control cell cycle progression. (wikipedia.org)
For instance, RUNX2 is involved in the progression of various tumor types, such as osteosarcoma, renal cell carcinoma, gastric cancer and breast cancer ( 15 - 20 ). (spandidos-publications.com)
Of note, other studies have also indicated the involvement of RUNX2 in breast cancer stem cells (BCSCs) and breast cancer progression ( 22 , 23 ). (spandidos-publications.com)
Gene networks reveal stem-cell state convergence during preneoplasia and progression to malignancy in multistage skin carcinogenesis. (ucsf.edu)
Hitherto the involvement of the MECs in mammary gland orchestration and morphogenesis [ 4 ] and in affording fortification against tumour progression and invasion [ 5 , 6 ] has re-established the attention in studying and characterizing the MECs. (biomedcentral.com)
Developmental progression of fetal HEB(-/-) precursors to the pre-T-cell stage is restored by HEBAlt. (sunnybrook.ca)
Accumulating evidence shows that cancer stem cells are key drivers of tumor formation, progression, and recurrence. (hindawi.com)

Murine mammary stem/progenitor cell isolation: Different method matters? (springeropen.com)
Murine mammary stem/progenitor cell isolation has been routinely used in many laboratories, yet direct comparison among different methods is lacking. (springeropen.com)
Murine mammary stem/progenitor cell isolation has been routinely used in many laboratories since the first two reports of mammary stem cell isolation in 2006 (Shackleton et al. (springeropen.com)
The paucity of murine models of this "luminal" tumor subtype has hindered studies of factors that promote their pathogenesis and modulate responsiveness to estrogen-directed therapeutics. (biomedcentral.com)
While promising, these cells were solely murine-derived, limiting their applicability to humans. (papersowl.com)
Protease inhibition did not affect invasion of TGFβ-1 treated normal mammary epithelial cells, but reduced the invasion of murine breast cancer cells. (biomedcentral.com)

Dll1-Mediated Notch Signaling Drives Tumor Cell Cross-talk with Cancer-Associated Fibroblasts to Promote Radioresistance in Breast Cancer. (utah.edu)
To intelligently employ bio-signaling molecules in one's medical practice (or one's home skincare regimen), there should be familiarity with the significant differences among the bio-signals patterns produced by adipose (fat) stem cells and bone marrow mesenchymal stem cells, and for completeness, fibroblasts. (barefacedtruth.com)
MSC co-culture with skin fibroblasts from mitochondrial disease patients rescued aberrant mitochondrial morphology from a fission state to a more fused appearance indicating an effect of MSC co-culture on host cell mitochondrial network formation. (frontiersin.org)
Studies of Fer-deficient mice have provided new insights into the role of Fer kinase in inflammation, growth factor signaling in fibroblasts, and regulation of mast cell activation and migration. (queensu.ca)

Profoundly different patterns of potency and division are exhibited by mammalian embryonic and adult stem cells. (iospress.com)
On a macro scale, the two classifications of stem cells are embryonic and adult stem cells. (papersowl.com)
To delve further into the specifics, embryonic and adult stem cells fall into potency categories reflecting their ability to differentiate: totipotent, pluripotent, multipotent, and unipotent. (papersowl.com)

In vivo, stable ERα expression has been reported in patient-derived xenograft (PDX) models, especially in tumor cells introduced via intraductal transplantations 8 , 9 and these findings have suggested a strong microenvironment-dependent dynamic component in the regulation of ERα expression. (nature.com)
Scientists explored the role of the Notch pathway within the mammary epithelial hierarchy by showing that knockdown of the canonical Notch effector Cbf-1 in the MaSC-enriched population results in increased stem cell activity in vivo as well as the formation of aberrant end buds, implying a role for endogenous Notch signaling in restricting MaSC expansion. (genengnews.com)
Conversely, Notch was found to be preferentially activated in the ductal luminal epithelium in vivo and promoted commitment of MaSCs exclusively along the luminal lineage. (genengnews.com)
Subsequent in vitro and in vivo studies have demonstrated the initial steps of cancer cell invasion and dissemination by disrupting the integrity of the myoepithelial belt that encircles the LECs [ 7 , 8 , 9 , 10 , 11 ]. (biomedcentral.com)
Together, these data suggest that NOTCH1 contributes to mammary tumor initiating activity in vitro and poten tially in vivo. (hdac-inhibitors.com)
In addition, NK cells stimulated with lunasin plus cytokines displayed higher tumoricidal activity than those stimulated with cytokines alone using in vitro and in vivo tumor models. (weeksmd.com)
Employing in vitro cell culture of inherited mitochondrial disease and an in vivo animal experimental model of low-grade inflammation (high fat feeding), we show human-derived MSCs to alter mitochondrial function. (frontiersin.org)
Until recently, it was believed that they were tissue-specific…however, this concept has been challenged… (multipotent cells) can differentiate in vitro and in vivo into various cell types not only from the tissue of origin" (Can/Hematol 2008). (papersowl.com)
Notch signals are required for in vitro but not in vivo maintenance of human hematopoietic stem cells and delay the appearance of multipotent progenitors. (sunnybrook.ca)
In vivo experiments were conducted using a co-implantation xenograft mouse model with live cell imaging and immunohistochemistry. (cdc.gov)

Astaxanthin modulates immune response, inhibits cancer cell growth, reduces bacterial load and gastric inflammation, and protects against UVA-induced oxidative stress in in vitro and rodent models. (drcalapai.com)
We then tested whether NOTCH1 activity was required for mammary tumor initiating cell activity by using an in vitro tumorsphere assay. (hdac-inhibitors.com)
Importantly, the ability of these VEGFR Inhibitors cells to form tumorspheres in vitro remains dependent on the expres sion of intracellular NOTCH1, as treatment of primary mammary tumor cells with doxycycline results in a 75% decrease in the number of tumorspheres. (hdac-inhibitors.com)
Marking a vast leap in reproductive therapy, it became possible to isolate these human embryonic stem cells for reproductive purposes, such as in vitro fertilization. (papersowl.com)
Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature. (sunnybrook.ca)
Although other irritants in the smoke may have contributed to the incident, there is supporting evidence that stable strontium can stimulate the release of histamine from mast cells in vitro (ATSDR 2001e). (cdc.gov)

There are multipotent stem cells that reside in mammary tissue (MaSCs) with the ability to self-renew and even differentiate into all types of mammary cells. (eurekaselect.com)
To identify, characterize, and differentiate MaSCs, a variety of methods have been used to understand the signal transduction pathways that promote their self-renewal and their cellular fate. (eurekaselect.com)

The effects of tetrandrine on cell growth, cell viability, cell stemness capacity, cell migration, and cell invasion, as well as the molecules involved in these processes, were investigated in a cell culture system. (stemcellsciencenews.com)

and human embryonic stem cells(hESCs). (sigmaaldrich.com)

We employ RNA interference, cell biology, transgenic mouse models, and tumor xenografting in the course of these studies. (queensu.ca)

To gain insight into the molecular mechanisms whereby lycopene and other carotenoids may exert their chemoprotective effects, we and others performed a series of studies that used a large panel of cancer cell lines of different lineages and animal models of human cancer. (drcalapai.com)
C3H/10T1/2 cells can be induced to differentiate into multiple stem cell lineages. (naturalnews.com)
Cells of epithelial lineages can go through phenotypic changes to get mesenchymal features via an epithelial-to-mesenchymal changeover (EMT) plan (Kalluri and Weinberg 2009 Full EMT takes place during mesoderm or neural crest development to generate completely dedicated mesenchymal cell types (Thiery et al. (buyresearchchemicalss.net)

Inflammatory processes, such as secretion of IL-8 from the gastric epithelium in response to chronic chemokine or antigen exposure, serve both as a chemoattractant for white blood cells and a prerequisite for gastric carcinogenesis. (drcalapai.com)

The mammary gland is a dynamic organ that undergoes extensive morphogenesis during the different stages of embryonic development, puberty, pregnancy, lactation and involution. (eurekaselect.com)

Gas6 is dispensable for pubertal mammary gland development. (machadolab.org)

The main objective of applying stem cells in conjunction with NPs is to control cellular activity, mainly the regenerative capacity of mammary tissue against diseases such as mastitis or to optimize milk production. (eurekaselect.com)
Mesenchymal stem cells (MSCs) are the most commonly used cells in tissue engineering and regenerative medicine. (frontiersin.org)

Cancer cell biology takes advantage of identifying diverse cellular signaling pathways that are disrupted in cancer. (eurekaselect.com)
Signaling pathways are an important means of communication from the exterior of cell to intracellular mediators, as well as intracellular interactions that govern diverse cellular processes. (eurekaselect.com)
Cripto-1 overexpression in U87 glioblastoma cells activates MAPK, focal adhesion and ErbB pathways. (eurekaselect.com)
My lab studies tyrosine kinase signaling pathways that regulate cell motility, tissue invasion and inflammation. (queensu.ca)
Lipids, amino acids, and nucleotides necessary for the biosynthesis of the daughter cells are mostly provided by intermediate metabolites of these pathways. (hindawi.com)

Enhanced TGF-� Signaling Contributes to the Insulin-Induced Angiogenic Responses of Endothelial Cells. (ucsf.edu)
The Lin − was defined as cells depleted of endothelial (CD31) and hematopoietic (CD45 and TER119) cells. (springeropen.com)
Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells. (bnwax.com)
The inflammatory kinase MAP4K4 promotes reactivation of Kaposi's sarcoma herpesvirus and enhances the invasiveness of infected endothelial cells. (bnwax.com)
While both processes result in new blood vessel formation, vasculogenesis occurs mainly during embryonic development and is facilitated by EPCs, while angiogenesis takes place via "sprouting" of endothelial cells , which allows new blood vessels to take shape from already existing blood vessels. (naturalnews.com)
Finally, the researchers reported that the mRNA levels of endothelial markers - namely, the platelet endothelial adhesive factor-1 (CD31), the endothelial cell-specific molecule-1 (ESM-1), and the vascular von Willebrand factor (vWF) in mouse 4T1 mammary tumor tissue - significantly increased after epimedin C treatment. (naturalnews.com)
The mechanism of how the calcification signal from endothelial cells is transmitted to vascular smooth muscle cells (VSMCs) remains unknown. (spandidos-publications.com)

For instance, a recent study by our group reported the facilitating effect of RUNX2 during aggressiveness and chemoresistance of TNBC cells via activating MMP1, which was significantly associated with poor prognosis ( 21 ). (spandidos-publications.com)

When we improved the tissue mechanical dissociation by extending chopping time and increased agitation during the digestion process, we did obtain high cell yield (e.g., 3-33 million cells from two thoracic and two inguinal glands of a mouse). (springeropen.com)
Proteome Profiler Mouse Angiogenesis Array Kit (Catalog # ARY015) Desmoglein 3 promotes cancer cell migration and invasion by regulating activator protein 1 and protein kinase C-dependent-Ezrin activation. (bnwax.com)
In his talk, Dmitry is presenting the analysis of 2 novel knock-in mouse models that allow us to track and selectively eliminate senescent cells. (lifeboat.com)
With a novel orthotopic mouse model, progress has been made in identifying a breast cancer stem or initiating population from TN breast cancer patients. (hdac-inhibitors.com)
Discussion Notch1 has been shown to promote commitment of mouse mammary stem cells along the luminal lineage. (hdac-inhibitors.com)
Consistent with these and other studies, we show that constitutive expression of intra cellular NOTCH1 in the developing mouse mammary gland stimulates luminal fate, ultimately resulting in transformation of the mammary gland. (hdac-inhibitors.com)
Combining 2 existing chemotherapy drugs reduced the numbers of cancer stem cells and improved survival in a mouse model. (oncologynurseadvisor.com)
The researchers used a human breast cancer cell line and a mouse model to test their hypotheses. (oncologynurseadvisor.com)
The authors leveraged the WAP-T syngeneic mammary carcinoma mouse model and developed a strategy combining magnetic-activated cell sorting-based tumor cell enrichment with high-throughput transcriptome analyses. (stemcellsciencenews.com)

As the major cell precursors in osteogenesis, mesenchymal stem cells (MSCs) are indispensable for bone homeostasis and development. (nature.com)

The normal anatomy of the mammary gland following pubertal development is shown in the images below. (medscape.com)

Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs. (utah.edu)
Conditional loss of ErbB3 delays mammary gland hyperplasia induced by mutant PIK3CA without affecting mammary tumor latency, gene expression, or signaling. (ucdenver.edu)
5-azacytidine and butyrate directly affect the ability of stem cells to spread as myoepithelial cells and to alter gene expression to promote growth. (oncologynurseadvisor.com)

Single-cell RNA sequencing reveals localized tumour ablation and intratumoural immunostimulant delivery potentiate T cell mediated tumour killing. (utah.edu)
Nonetheless, MECs were also found to exhibit cancer-promoting effects and provoke tumour invasion and dissemination by displaying distinct cancer chemokines. (biomedcentral.com)
Therefore, damaging the MECs layer resulted in the release of various factors (such as SDF1/CXCL12, CXCL14, MMP, and tenascin) [ 11 , 12 ] with a potential to modify the tumour microenvironment and facilitate the paracrine communication between the tumour epithelial cells and the enclosed stroma enhancing the tumour aggressiveness [ 11 , 13 ]. (biomedcentral.com)

Multipotent stem cells are capable of forming many, but not all, cell types of the body. (papersowl.com)

The work spotlights the potential importance of deregulated Notch in ductal precursor cells as a forerunner to breast cancer, according to the team. (genengnews.com)
The ductal system is coated by a chain of luminal epithelial cells (LECs) situated on a layer of myoepithelial cells (MECs) and encompassed by a distinguished basement membrane. (biomedcentral.com)
Two major cell types cooperate to build up the ductal units: inner polarized luminal epithelial cells (LECs) embraced by a layer of myoepithelial cells (MECs), both cell types are rimmed by a cohesive basement membrane (BM) [ 2 ]. (biomedcentral.com)
Inside the bud, a rudimentary mammary ductal system is formed, which is present at birth. (medscape.com)

2013 high light the exceptional lineage plasticity of somatic cells. (buyresearchchemicalss.net)

Oligopotent stem cells, though not often widely discussed, have the ability to differentiate into a few cells (Kara et al. (papersowl.com)

Runt‑related transcription factor 2 (RUNX2), a pivotal transcription factor in mammary gland and bone development, has also been related to metastatic cancer and BCSCs. (spandidos-publications.com)
Do you know that fat stem cells and bone marrow stem cells are really, really different? (barefacedtruth.com)
It's no secret to readers here that DrJohn and DrGeorge have day jobs as stem cell researchers, both in the lab and the clinic, and have developed skincare products whose hero ingredients (human growth factors, cytokines, mRNAs,miRNAs, etc) derive from stem cells on permanent loan from bone marrow of young adult humans. (barefacedtruth.com)
Bone marrow (BM)-derived macrophages and RAW264.7 cell line co-cultured with MDSCs, which were induced at different conditions. (biomedcentral.com)
Since the researchers already knew that vasculogenesis plays a pivotal role in bone formation, they used a bone morphogenetic protein 2 (BMP2)-induced ectopic bone formation model and mice 4T1 breast cancer cells co-implanted with luciferase-labeled C3H/10T1/2 cells (4T1 + C3H/10T1/2-Luc) model for their study. (naturalnews.com)

Role of EphB3 Receptor in Mediating Head and Neck Tumor Growth, Cell Migration, and Response to PI3K Inhibitor. (ucdenver.edu)
Hallmarks of EMT were analyzed by molecular- and cell-biologic analyses including real-time cell migration/invasion assays. (biomedcentral.com)

In this study, we investigated programmed death-ligand 1 (PDL-1) as a potential biomarker for CAF-induced chemoresistance and evaluated its role and the underlying mechanisms of chemoresistance in non-small cell lung cancer (NSCLC). (cdc.gov)
RESULTS: We demonstrated that chemotherapy-stimulated CAFs promoted tumorigenic and stem cell-like properties of NSCLC cells, which contribute to their chemoresistance. (cdc.gov)
Silencing PDL-1 expression suppressed CAFs' ability to promote stem cell-like properties and invasiveness of lung cancer cells, favoring chemoresistance. (cdc.gov)

In contrast, adult stem cells can only give rise to differentiated somatic cells of the particular tissue from which these cells originated (Can/Hematol 2008). (papersowl.com)
Using these definitions, embryonic stem cells can be classified as totipotent or pluripotent at different developmental stages and locations, whereas adult stem cells can be categorized as pluripotent, multipotent, or unipotent (Figure 1). (papersowl.com)
In the early 1980s, scientists began to exploit two major classes of stem cells: embryonic and non-embryonic, or adult stem cells. (papersowl.com)
In a study that was published in 2018, Van de Walle and Dr Daryl Nydam, a professor in population medicine and diagnostic sciences at Cornell, had investigated the bovine mammary "secretome" - that is, all the compounds secreted from adult stem cells in mammary tissue. (dairyglobal.net)

CAFs subsequently secreted factors that promoted expansion and self-renewal of CSCs. (mdc-berlin.de)

Hematopoietic stem cells, which can produce several different types of blood cells, are prime examples of multipotency. (papersowl.com)

In this review, we provided an overview of the myoepithelial cells' histogenesis, molecular and biological markers, and physiological commitments. (biomedcentral.com)
Previously, he was the Principal Investigator and lead of his lab at The Institute of Molecular and Cell Biology (IMCB) . (lifeboat.com)
We hypothesized that promoting active patient participation in the management of their disease offering access to NGS testing and to the subsequent medical interpretation and recommendations provided by a multidisciplinary molecular advisory board (MAB) could contribute to progressively overcome this challenge. (bvsalud.org)
Before delving into the molecular biology and therapeutic potentials of induced pluripotent stem cells, it is crucial to provide foundational definitions and descriptions. (papersowl.com)
Of particular interest are discovering the molecular mechanisms controlling cell motility and invasion in mast cells and cancer cells. (queensu.ca)
Most current chemotherapy does not kill the stem cells, which are the cells of origin, only the tumor mass," said Muthusamy Thangaraju, PhD, an associate professor in the Department of Biochemistry and Molecular Biology at the Medical College of Georgia and Georgia Cancer Center at Augusta University. (oncologynurseadvisor.com)

Cell death plays a pivotal role in the maintenance of tissue homeostasis. (researchgate.net)
Mitochondrial function or the ability to generate energy through OXPHOS (oxidative phosphorylation) is vital for cell homeostasis and its dysfunction has been linked to the pathogenesis of nearly all chronic diseases ( Pieczenik and Neustadt, 2007 ). (frontiersin.org)
Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice. (machadolab.org)
These hormones regulate the body's growth, and are involved in cell to cell communication, control metabolic activity, sleep-wake homeostasis, and altered regulation or dysregulation of adaptive response in various physiologic and pathophysiologic states. (medscape.com)

A431 human epithelial carcinoma cells were either left untreated or treated with 200 ng/mL recombinant human (rh) EGF (Catalog # 236-EG) for 5 minutes. (bnwax.com)
Deletion of p53 and Hyper-Activation of PIK3CA in Keratin-15+ Stem Cells Lead to the Development of Spontaneous Squamous Cell Carcinoma. (ucdenver.edu)
Epidermal growth factor receptor signaling in precancerous keratinocytes promotes neighboring head and neck cancer squamous cell carcinoma cancer stem cell-like properties and phosphoinositide 3-kinase inhibitor insensitivity. (ucdenver.edu)
Normal epithelial cells and carcinoma cells can acquire invasiveness by epithelial-to-mesenchymal transition (EMT), a process of considerable cellular remodeling. (biomedcentral.com)

Additionally, MECs contributed effectively to maintaining the right luminal cells' polarization and further separating them from the adjacent stroma by making an integrated fence. (biomedcentral.com)
Indeed, disruption of the MECs layer was reported to facilitate the invasion of the cancer cells to the surrounding stroma. (biomedcentral.com)

Cancer cells can acquire this de-differentiated state through epithelial-to-mesenchymal transition (EMT). (biomedcentral.com)

Whether within the body or in a laboratory flask, growth factors and cytokines patterns are determined by the genetic material within the cells that produce them. (barefacedtruth.com)
These are the three most commonly cultured cells used to produce growth factors and cytokines for skincare products. (barefacedtruth.com)
Overall, the presence of stem cells is essential for proper human development and function as they contribute to the growth, maintenance, and repair of numerous physiological systems (Kara et al. (papersowl.com)
MicroRNA miR-93 promotes tumor growth and angiogenesis by targeting integrin-β8. (sunnybrook.ca)
Combined inhibition of DNMT and HDAC blocks the tumorigenicity of cancer stem-like cells and attenuates mammary tumor growth [published online ahead of print April 5, 2016]. (oncologynurseadvisor.com)
low dosages of GO promoted cell growth, while high doses induced cell death. (biomedcentral.com)

First identified in embryonic stem cells, SEs mediate pluripotent state maintenance by promoting the expression of pluripotent identity genes, including Oct4 , Sox2 , Nanog and Klf4 . (nature.com)
To elucidate the role of the p53-repressive effect of ΔNp63α in radiation response, we performed a p63-siRNA knockdown experiment using human mammary epithelial cells (HMECs) expressing ΔNp63α and then performed ectopic and entopic expression experiments using human induced pluripotent stem cells (hiPSCs). (researchsquare.com)
Embryonic stem cells have the unique ability to exist in an undifferentiated state indefinitely while also being pluripotent. (papersowl.com)
A pluripotent stem cell has the potential to form all cells of the embryo, but cannot form a placenta. (papersowl.com)
Takahashi and Yamanaka 2006 as well as the lifetime of pluripotent cells in physiological adult tissue (Roy et al. (buyresearchchemicalss.net)

The establishment of ERα + luminal breast cancer cell lines has turned out to be a challenging task for reasons not entirely clear. (nature.com)
Studies on the transcriptomic profiles of the clonal luminal breast cancer cell lines suggest that these cell lines do not recapitulate well the established luminal tumor subtype 7 . (nature.com)
Cancer Cell International. (elsevierpure.com)
Cancer Cell , 40 (12), 1448-1453. (utah.edu)
The aim of this work was to study the cytotoxic potential of extracts from this mushroom in various cancer cell lines. (drcalapai.com)
In the present studies, we utilized prostate cancer cell culture models to elucidate the mechanisms of action of broccoli-derived phytochemicals 3,3′-diindolylmethane (DIM) and indole-3-carbinol (I3C). (drcalapai.com)
Cancer Cell CD44 Mediates Macrophage/Monocyte-Driven Regulation of Head and Neck Cancer Stem Cells. (ucdenver.edu)
One such peptide, Lunasin , has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. (weeksmd.com)
We have research ongoing to study functions of kinases and adaptor proteins targeted to membranes via their F-BAR domains in mast cells and cancer cell models. (queensu.ca)
Researchers verified the potency of GO-Y030 against a CSC population and observed that GO-Y030 suppressed CSC sphere-forming ability in several cancer cell lines. (stemcellsciencenews.com)
Our results demonstrate an important role of lysosomal proteolysis in cellular remodeling during EMT and a pivotal contribution of lysosomal cysteine cathepsins to TGFβ-1 induced acquisition of breast cancer cell invasiveness. (biomedcentral.com)

In this review, we provide a current overview of the application of nanomaterials on stem cells, specifically bovine stem cells in both the mammary gland and milk, as they are a potential source of cell progenitors with potential characteristics and could be used in potential therapies based on these cells. (eurekaselect.com)
Additional confusion surrounds stem-cell surrogates, cache and reserve cells having some characteristics of stem cells and not others. (iospress.com)

Anatomy35

Organisms8

Diseases6

Chemicals and Drugs19

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes27

Disciplines and Occupations4

Technology, Industry, Agriculture1

Tumorigenesis6

Differentiation26

Tumors11

Tissues12

Apoptosis14

Proliferation11

Basal12

Transformed mammary tumor2

Progenitor cells5

Cancers6

Metastasis9

Malignant6

Premalignant mammary2

Progression9

Murine6

Fibroblasts4

Embryonic and adult stem3

Vivo10

Vitro6

MaSCs2

Stemness1

Human embryonic stem1

Transgenic1

Lineages3

Epithelium1

Morphogenesis1

Gland development1

Regenerative2

Pathways5

Endothelial7

Significantly1

Mouse9

Mesenchymal stem1

Pubertal1

Gene expression3

Tumour3

Multipotent stem1

Ductal4

Plasticity1

Differentiate1

Bone5

Migration2

Chemoresistance3

Adult stem4

Self-renewal1

Hematopoietic1

Molecular6

Homeostasis4

Carcinoma4

Stroma2

Epithelial to Mesenchymal Transition1

Growth6

Pluripotent5

Cancer Cell11

Characteristics2