Mammalian functional clone cells. Medical search. Frequent questions

There have been three types of gap junctional proteins (connexins) identified in the mammalian lens: Cx43, Cx46, Cx50. (rosalindfranklin.edu)
One of the major aims of Dr. Ebihara's research project are to obtain a more detailed understanding of the biophysical properties of cloned lens gap junctional proteins and to localize the molecular determinants of permeability and gating of the gap junctional channels. (rosalindfranklin.edu)
Structurally, the IL-17 cytokine family members have no sequence similarity to any other known cytokine or other mammalian proteins [ 2 ]. (hindawi.com)
Use of recombinant proteins varies widely - from functional studies in vivo to large-scale protein production for structural studies and therapeutics. (thermofisher.com)
This work identifies Drosophila S2 cells as a clinically-relevant platform suited for the production of 'difficult-to-make' proteins from Plasmodium parasites, and identifies a PfRH5 sequence variant that can be used for clinical production of a non-glycosylated, soluble full-length protein vaccine immunogen. (nature.com)
The reversible phosphorylation of proteins on serine, threonine, and tyrosine residues represents a fundamental strategy used by eukaryotic organisms to regulate a host of biological functions, including DNA replication, cell cycle progression, energy metabolism, and cell growth and differentiation. (rupress.org)
The delivery of proteins to the presynaptic terminals of guinea pig retinal ganglion cells by two of the major components of axonal transport, and the subsequent persistence and turnover of those proteins were examined in this study. (shengsci.com)
Ganglion cell proteins were radiolabeled by intravitreal injection of radiolabeled am. (shengsci.com)
These studies aid in understanding how genes determining cell surface proteins can affect interactions between cells and confer viral disease resistance. (usda.gov)
Using the yeast structure as a template, we have modeled the homologous domains from elongin A and CRSP70 and identified a conserved positively charged patch on the surface of all three proteins, which may be involved in conserved functional interactions with the transcriptional machinery. (embl.de)
To determine the reason for this species specificity, chimeras were constructed linking three structurally independent regions of the TFIIS proteins from yeast and human cells. (embl.de)
We have shown that all mammalian Cbl proteins mediate ubiquitination and degradation of the activated EGFR as well as other components of the signaling complex. (cancer.gov)
Ongoing work is focused on understanding the biochemical and physiologic functions of the three mammalian Cbl proteins in epithelial cells and elucidating the differences in their specificity and/or function. (cancer.gov)
We also provide recombinant proteins and cells expressing target proteins for functional assays. (jubilantbiosys.com)
Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. (qiagen.com)
They are recruited from the cytosol onto cell membranes in the inactive form, are activated by guanine nucleotide exchange factors and then bind a large number of Rab effector proteins. (sputnic-group.ru)
Rab10, first isolated from a clone of Madin-Darby Canine kidney (MDCK) cells belongs to a subfamily of Rab proteins that includes Rab8 and Rab13. (sputnic-group.ru)
This subfamily of Rabs represents the closest mammalian relatives of the Sec4p, one of the first described Rab proteins, which mediates polarized transport from the trans-Golgi network (TGN) to the site of bud formation in yeast. (sputnic-group.ru)
We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. (biomedcentral.com)
LHCGR recombinant proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster Ovary cells in suspension culture. (biomedcentral.com)
Cell line development is the process of creating stable cell lines for use in a variety of applications, including the creation of biologics (recombinant proteins and monoclonal antibodies), drug screening, and gene functional investigations. (alliedmarketresearch.com)
The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)

Genes known to be highly expressed in β-cells were represented at a high frequency, namely insulin (15 of 80 clones), islet amyloid polypeptide (8 of 80 clones), proinsulin convertase 1 (6 of 80 clones), and neuropeptide Y (2 of 80 clones). (diabetesjournals.org)
The technology typically involves obtaining antibody genes from source cells including hybridomas and phage display libraries, amplifying and cloning the genes into an expression vector, introducing the vector into a host (bacteria, yeast, or mammalian cell lines), and achieving adequate expression of the functional antibody. (labmanager.com)
Recent advances in functional genomics allow us to estimate the expression of several thousands of genes in the mammalian genome. (ejbiotechnology.info)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
To resolve misconfigured cells we include a Bayesian fitted online artificial that is a different restriction to perform the genetic and the yeast genes, Qbeing P-splines. (scoutconnection.com)
My group cloned two of the three mammalian Cbl genes (Cbl-b and Cbl-c). (cancer.gov)
Jubilant Biosys has the experience in handling target genes that encompass kinases, carboxylases, ion-channels, GPCRs, lipases, etc. in generating stables in different cell backgrounds by using plasmid-mediated or lentiviral mediated transfection. (jubilantbiosys.com)
Expression of cloned envelope protein genes from the flavivirus tick-borne encephalitis virus in mammalian cells and random mutagenesis by PCR. (microbiologyresearch.org)
RT-PCR and western blot results showed the specific and strong detection of hisADC genes in hisADC PT67 transfected cells compared with normal control and pLXSN transfected PT67 cells. (elsevierpure.com)
He cloned, characterized the family of phosphoglycerate kinase (PGK) genes and pseudogenes. (madridge.org)
The ABCs of Gene Cloning, Third Edition contains updates including a tutorial chapter on gene-vector construction, methodologies on exome sequencing in finding disease genes, revised topics on gene therapy and whole genome sequencing, new developments for gene targeting and genome editing, as well as the current state of next generation sequencing. (lu.se)
With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)

These include cell-free extracts ( in vitro expression systems), and bacterial, yeast, insect, and mammalian cell systems, each with its own advantages and drawbacks. (thermofisher.com)
Domain III has previously been shown to be readily exchanged between mammalian and yeast factors. (embl.de)
We describe the cognate cDNA cloning and characterization of two subunits (hTFIIIC63 and hTFIIIC102) that are present within a DNA-binding subcomplex (TFIIIC2) of TFIIIC and are related in structure and function to two yeast TFIIIC subunits (yTFIIIC95 and yTFIIIC131) previously shown to interact, respectively, with the promoter (A box) and with a subunit of yeast TFIIIB. (embl.de)
SureVector next-gen cloning kits now have standard parts of mammalian, yeast and E.coli cells and those can be customized to different biological components using Eurofins technology. (eurofinsgenomics.com)

Cloning and functional characterization of GNPI2, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin. (nih.gov)
Functional characterization of an organic cation transporter (hOCT1) in a transiently transfected human cell line (HeLa). (drugbank.com)
Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III. (embl.de)
Our service comes with a full package - from construct design, gene cloning and expression, custom protein production , protein characterization to crystal hit screening and optimization, x-ray diffraction data collection and structure solving. (jubilantbiosys.com)

We and other groups discovered the human IL-17F gene from a human EST sequence, a genomic DNA clone, and T-cell cDNA sequences in 2001 [ 3 , 8 , 9 ]. (hindawi.com)
Gateway technology allows highly efficient transfer of the gene of interest into any of a number of expression vectors from a single entry clone. (thermofisher.com)
This study describes the molecular cloning of the non-coding exon 1 and approximately 2 kb of 5' flanking region of the human PDGF alpha R gene. (nih.gov)
This 5' flanking region is a functional promoter of the PDGF alpha R gene as concluded from its capacity to drive luciferase reporter gene expression in an orientation dependent way. (nih.gov)
In addition, the morphogen retinoic acid, alone or in combination with dibutyryl cAMP, gives a 22-fold induction of PDGF alpha R gene promoter activity in human teratocarcinoma cells. (nih.gov)
The human glucosamine-6-phosphate deaminase gene: cDNA cloning and expression, genomic organization and chromosomal localization. (nih.gov)
Via positional cloning, we show that mutations in a zebrafish LTCC encoding gene, cav1.3a , underlie the auditory-vestibular defects of gemini ( gem ) circler mutants. (jneurosci.org)
Featuring high-contrast multichannel fluorescent and white light imaging that allows for accurate single-cell detection and proof of monoclonality at day 0, the instrument allows you to streamline your workflow with comparative confluence assays to identify and verify gene edits. (moleculardevices.com)
Techniques such as microarrays, expressed tag sequencing (EST), serial analysis of gene expression (SAGE), subtractive cloning and differential display (DD), and two-dimensional electrophoresis gel have been extensively used to screen and analyze parallel gene expression. (ejbiotechnology.info)
Preferably, the human beta interferon gene is introduced into the cells of a mouse. (justia.com)
Apparently, Kawade had not determined whether the chimeric gene was actually expressed in the mice, but had already been able to express it in cultured cells induced by Cd.sup.2+ or by heat. (justia.com)
Expression was not reported, and the purpose of the study was to show that the technique could be used to study gene action during mammalian development. (justia.com)
Staehelin suggested that an animal could be protected against viral infection by inserting a gene encoding the Mx protein into that animal, preferably at the single cell embryo stage. (justia.com)
Selected mutations were introduced into a prM-E gene cassette and expressed transiently in COS cells to investigate whether the mutations impaired prM/E association or secretion. (microbiologyresearch.org)
This study first optimized the conditions for efficient hexahistidine tagged human ADC (hisADC) gene delivery into mouse fibroblast cell line (NIH3T3) using retroviral vector (pLXSN). (elsevierpure.com)
He designed and constructed The PGK1-Neo Shuttle which is being used around the world for gene therapy, to engineer, from embryonic stem cells, transgenic and knockout animals to understand the causes and mechanisms of all human diseases and find cures. (madridge.org)
He cloned the pgk-2 gene, showing for the first time that gene duplication, by retroposition more than 100 million years ago, have been used as a mechanism for evolutionary diversification and that mammalian genomes are fluid creating new species. (madridge.org)
For instance, in April 2020, CEVEC Pharmaceuticals GmbH announced the launch of the ELEVECTA platform, which will produce stable cell lines for AAV (Adeno-associated viral) vector for gene therapy. (alliedmarketresearch.com)
Identification of the Menkes gene by positional cloning has enabled molecular diagnosis of females who carry the gene and at-risk fetuses in certain families, enhancing preventive efforts. (medscape.com)
Clear and concise, this easy-to-use book offers an introductory course on the language of gene cloning, covering microbial, plant, and mammalian systems. (lu.se)
It presents the nuts and bolts of gene cloning in a well-organized and accessible manner. (lu.se)
Part I of this book outlines the essentials of biology and genetics relevant to the concept of gene cloning. (lu.se)
Part II describes common techniques and approaches of gene cloning, ranging from the basic mechanics of DNA manipulation, vector systems, process transformation, to gene analysis. (lu.se)
With more than 140 illustrations, this new edition provides an invaluable text for students and anyone who have interest in gaining proficiency in reading and speaking the language of gene cloning. (lu.se)
Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

Using this binding mechanism, an antibody can tag a microbe or an infected cell for attack by other parts of the immune system, or can neutralize it directly (for example, by blocking a part of a virus that is essential for its invasion). (wikipedia.org)
The terms antibody and immunoglobulin are often used interchangeably, [1] though the term 'antibody' is sometimes reserved for the secreted, soluble form, i.e. excluding B-cell receptors. (wikipedia.org)
Analysis following immunization of rabbits identified quantitative and qualitative differences in terms of the functional IgG antibody response against the P. falciparum parasite. (nature.com)
Moreover, the kinetic constraints imposed by such rapid erythrocyte invasion mean that extremely high concentrations of functional antibody are required to neutralize the parasite 6 . (nature.com)
Bone marrow cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat. (bdbiosciences.com)
The MEC13.3 antibody specifically recognizes CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). (bdbiosciences.com)
We also show that a nanobody (a single-domain antibody) that interferes with FluPol A dimerization inhibits the synthesis of vRNA and, consequently, inhibits virus replication in infected cells. (nature.com)
Activation of endothelial cells via antibody-enhanced dengue virus infection of peripheral blood monocytes. (microbiologyresearch.org)
Using microfluidic, single-cell molecular cloning and screening technologies to enable antibody repertoire mining and discovery, Memo Therapeutics has identified a promising antibody candidate against SARS-CoV-2 and the South African variant (B.1.351). (drugtargetreview.com)
The antibody repertoires are subsequently screened in single-cell format using microfluidic screening technology that can assess millions of candidate antibodies directly in functional assays, resulting in recombinant clonal cell lines expressing monoclonal antibodies (mAbs) with the desired functional properties. (drugtargetreview.com)

We use a combination of cell-based and in vitro assays to show that the interface of the FluPol A dimer is required for vRNA synthesis during replication of the viral genome. (nature.com)

In sexual reproduction, clones are created when a fertilized egg splits to produce identical (monozygous) twins with identical genomes. (who.int)

In addition, Addgene has many luciferase expression constructs available that are driven by a strong constitutive promoter and can be used to monitor transfection or viral infection efficiency, to study changes in cell physiology, or as a reference level of expression. (addgene.org)
The complete hisADC pLXSN clone was transfected into PT67 cell line following CalPhos Mammalian transfection method. (elsevierpure.com)
11 ] first reported the production and secretion of soluble LH receptor following transient transfection of a naturally truncated variant of the receptor in COS cells. (biomedcentral.com)
Transfection, selection, and single-cell cloning are all part of the cell line generation process. (alliedmarketresearch.com)

Sia D, Villanueva A, Friedman S and Llovet J: Liver cancer cell of origin, molecular class, and effects on patient prognosis. (spandidos-publications.com)
Transiently transfected HeLa cells represent a useful tool in studying the interactions and kinetics of organic cations and other xenobiotics with hOCT1 and in understanding the molecular events involved in organic cation transport. (drugbank.com)
The earliest biochemical evidence on the existence of cell-free soluble LH receptor was the purification of an hCG-binding protein, relative molecular mass of 65K (M r , 65K) from porcine follicular fluid and was based on gel filtration followed by affinity chromatography [ 9 ]. (biomedcentral.com)
Reviews in Cell Biology and Molecular Medicine articles are self-contained treatments of a given topic, usually with over 100 references and 10 to 20 figures and tables, making this an authoritative reference source for undergraduate and graduate students, faculty, librarians and researchers. (lu.se)

It enables researchers to isolate and dispense single cells for downstream applications such as clonal expansion, monoclonality assurance, and single-cell genomics. (moleculardevices.com)
Agilent Technologies Inc. has collaborated with Eurofins Genomics to expand Agilent's SureVector cloning system. (eurofinsgenomics.com)

Here, we tested whether ALKBH6 plays a significant role in preventing alkylating DNA damage and decreasing genomic instability in pancreatic cancer cells. (frontiersin.org)
Some pathological processes, for example, tumorigenesis and solid tumour growth, in which the former is derived from a single-cell and the latter has a mixed-cell problem, present new challenges to the limit of these functional genomic techniques. (ejbiotechnology.info)
To fully understand the functions of cells in tumorigenesis or in heterogeneous solid tumour masses, it is essential for scientists and physicians to develop a strategy to identify genomic expression profiles for a single-cell or for small numbers of cells. (ejbiotechnology.info)
In this article, we review recently developed methods that enable functional genomic analysis at the single-cell or multi-cell level. (ejbiotechnology.info)
In addition, the paper will review different techniques of single-cell genomic expression at the level of DNA, mRNA, protein and post-translational modifications. (ejbiotechnology.info)
In our third project we are using functional genomic approaches and tumor genetics to identify new therapeutic targets in triple-negative breast cancer cells. (cancer.gov)
Mutations encoding basic (Arg), non-polar (Leu and Pro) and uncharged polar (Asn, Gln and Tyr) amino acids at M39 were introduced into a DENV-2 genomic-length cDNA clone and their effects on virus replication were examined. (microbiologyresearch.org)

We have recently identified, for the first time, that bronchial epithelial cells are its novel cell source in response to IL-33 via ST2-ERK1/2-MSK1 signaling pathway. (hindawi.com)

1 Department of Cell Biology, University of Nijmegen, The Netherlands. (nih.gov)
The structural biology team provides the following functional services that help to accelerate small-molecule drug discovery and have a track record serving more than 20 pharmaceutical/biotech companies worldwide. (jubilantbiosys.com)
He contributed to the emergence of the field of stem cell biology & tissue engineering. (madridge.org)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

Many of the novel cDNA sequences that were highly represented in the library showed a relative specificity to β-cells compared with other tissues, including glucagonoma, liver, kidney, brain, 3T3 fibroblasts, and AtT20 corticotrophs, and warrant further investigation. (diabetesjournals.org)
However, it is difficult to capture the complexity of transcript isoform variance using these libraries, and large cDNA sequences are often difficult to clone into size-limited viral expression vectors. (cdc.gov)

The antiviral activity of various mammalian interferons is reviewed in Colowick, S. P. and Kaplan, N. O. Interferons, S. Pestka (Ed.), Methods in Enzymology. (justia.com)

There is the T5 promoter/lac operator transcription-translation system for expression in E. coli, the p10 promoter for baculovirus-based expression in insect cells, and the CAG (CMV/actin/globin) promoter for expression in mammalian cells. (qiagen.com)
The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E. coli , and baculovirus-infected insect cells, respectively (see figure pQE TriSystem. pQE TriSystem. "> pQE TriSystem ). (qiagen.com)
If expression in mammalian or insect cells is required - to obtain post-translational modifications, for example - the same construct can be used without the need for time-consuming subcloning procedures. (qiagen.com)
Vector pQE-TriSystem constructs can be transformed into E. coli , used as a shuttle vector for recombinant protein expression in insect cells, or transfected into mammalian cells. (qiagen.com)

The Gateway recombination cloning system is designed for highly efficient transfer of your DNA into any Gateway expression vector from a single entry clone. (thermofisher.com)
The chimeric class I glycoprotein was expressed in target cells using an ALV derived retrovirus vector (RCASBP). (usda.gov)

Together with B and T cells , antibodies comprise the most important part of the adaptive immune system . (wikipedia.org)
Initially, all antibodies are of the first form, attached to the surface of a B cell - these are then referred to as B-cell receptors (BCR). (wikipedia.org)
After an antigen binds to a BCR, the B cell activates to proliferate and differentiate into either plasma cells , which secrete soluble antibodies with the same paratope, or memory B cells , which survive in the body to enable long-lasting immunity to the antigen. (wikipedia.org)
Optionally, manufacturers can engineer the recombinant antibodies to have specific properties, such as higher binding affinity, improved stability, or the ability to target particular cell types (which is not possible with traditional antibodies). (labmanager.com)
Progress in this arena, however, is being made with a new generation of merozoite antigen targets identified in recent years that exhibit relatively low levels of polymorphism and against which functional neutralizing antibodies can be raised by vaccination. (nature.com)
Anti-merozoite vaccine studies have long relied on the standardized in vitro assay of growth inhibition activity (GIA) 12 , whereby purified IgG antibodies are tested against parasites cultured in human red blood cells (RBC) in the absence of other cell types. (nature.com)
The production of antibodies by plasma cells (also known as effector B cells) has first described by Astrid Fagraeus in 1948. (zen-bioscience.com)
Monoclonal antibodies to the FLAG epitope served to monitor cell surface expression for functional analysis of the BFIV21 class I glycoprotein. (usda.gov)
We have demonstrated that resistance to TRAIL-induced apoptosis can be overcome by co-incubation of the cells with chemotherapeutic agents, semi-synthetic retinoids (such as 4HPR), or molecularly targeted agents (such as anti-ErbB-2 antibodies). (cancer.gov)
Moreover, the market is expected to experience growth during the forecast period owing to the rise in development and demand for monoclonal antibodies around the globe, the rise in vaccine production, and technological innovations in cell lines and the membrane. (alliedmarketresearch.com)

Systematical profiling and comprehensive annotation of embryonic stem cells lincRNAs may not only bring a clearer big picture of these novel regulators but also shed light on their functionalities. (biomedcentral.com)

ONC201 kills breast cancer cells in vitro by targeting mitochondria. (cancer.gov)

The DispenCell™ Single-Cell Dispenser is an automated laboratory instrument designed for fast, easy and gentle single-cell isolation. (moleculardevices.com)
Executing basic techniques for recombinant protein production: cloning, DNA prep, growth and maintenance of E.coli laboratory strains, mammalian cell culture etc. (jobportale-stuttgart.de)
My laboratory studies signal transduction pathways that regulate growth and programmed cell death in epithelial cancer cells, with a focus on breast and ovarian cancer. (cancer.gov)

However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
The membrane anchor of SQR in mammalian mitochondria and proteobacteria, such as Escherichia coli , consists of two polypeptides (SdhC and SdhD) and contains one heme group. (lu.se)

1993. Inhibition of HIV-1 Replication in Cultured Cells with Antisense Oligonucleotides Encapsulated in Immunoliposomes. (justia.com)
In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)

In a second project we are investigating the induction of apoptosis by activation of death receptors for the ligand TRAIL in breast and ovarian cancer cells. (cancer.gov)
We have shown that most breast and ovarian cancer cell lines are resistant to the induction of apoptosis by TRAIL, the ligand for the death receptors DR4 and DR5. (cancer.gov)
He described functional polymorphisms in Interleukin-4, IL-13 and their receptors, thus making these molecules, with STAT 6, targets for therapeutic treatment of allergic patients and preventive strategies. (madridge.org)
2 Despite their function in membrane trafficking, Rab may also be involved in signal transduction by regulating the membrane trafficking of cell surface receptors for hormone, cytokine, and chemokine. (sputnic-group.ru)

We show that CSC are predominantly CD49f + proposing the use of CD44high/CD24low in combination with Ep-CAM/CD49f cell surface markers to further enrich for CSC.Our profiling demonstrates that both normal and breast cancer cells with the CD44high/CD24low phenotype have the highest stem/progenitor cell ability when used in combination with Ep-CAM/CD49f reference markers. (madridge.org)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)

Expression of the platelet-derived growth factor alpha receptor (PDGF alpha R) is strictly regulated during mammalian development and tumorigenesis. (nih.gov)

General Assembly the following year,3 and the World Medical Association's Resolution on Cloning, endorsed in 1997, have confronted the issue but lack binding legal force. (who.int)

1995) and subsequently in primary cells (Heyworth et al. (lu.se)

A polymerase chain reaction-based subtractive hybridization procedure was applied to cDNAs prepared from mouse insulinoma (βTC3) and glucagonoma (αTC2) cell lines to construct a library of cDNAs that are highly expressed in pancreatic β-cells. (diabetesjournals.org)
online artificial photosynthesis 2016: E22The applications for the complementary Building of fluid and resistive parameters are generally reasonably defined for a competing such indicator and a other construct cell. (scoutconnection.com)

And the hybridoma technology, described by Georges Köhler and Cesar Milstein in 1975, is based on the immunization of animals with the desired antigen, followed by the fusion of specific B lymphocytes with "immortal" myeloma cells. (zen-bioscience.com)
Functional analysis employing site directed mutagenesis identified BF amino acid residues forming serologic epitopes as well as residues important in antigen presentation to ALV induced cytotoxic T lymphocytes. (usda.gov)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

J Cell Biol. (spandidos-publications.com)
Annu Rev Cell Dev Biol. (spandidos-publications.com)
J Cell Biol (2000) 150 (2): F57-F62. (rupress.org)
Mol Cell Biol. (embl.de)

Lipofection was used to transiently transfect the hOCT1 plasmid DNA in a human cell line, HeLa. (drugbank.com)
14C-TEA uptake in hOCT1 plasmid DNA-transfected HeLa cells was trans-stimulated by unlabeled TEA and 1-methyl-4-phenyl-pyridinium. (drugbank.com)
Under this pact, Agilent will use Eurofins' technology to create custom components and assemble plasmid service for its SureVector next-generation cloning kits. (eurofinsgenomics.com)

A mutant connexin50 with enhanced hemichannel function leads to cell death. (rosalindfranklin.edu)
gem homozygous receptor mutant HCs display normal cell viability, afferent synaptogenesis, and peripheral innervation, yet exhibit strongly reduced extracellular potentials (∼50% of wild-type potentials). (jneurosci.org)
Apical FM1-43 uptake, however, is unaffected in gem mutant HCs, suggesting that mechanotransduction channels are functional. (jneurosci.org)
In MDCK cells, mutant variants of Rab10 affect early endocytic events. (sputnic-group.ru)
However, the E. coli cells did not show increased succinate dehydrogenase activity nor did the operon complement a sdhCDAB defective E. coli mutant [7]. (lu.se)

Most natural cloning occurs in those species that produce their descendants asexually, that is, without combining the male and female genetic material. (who.int)
Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)
The discovery platform captures and preserves entire B-cell repertoires from any donor species and any B-cell type in recombinant form for display using mammalian cells. (drugtargetreview.com)

The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)

CD31 is a 130 kDa integral membrane protein, a member of the immunoglobulin superfamily, that mediates cell-to-cell adhesion. (bdbiosciences.com)
In this way, Rabs co-ordinate the assembly of effector complexes and generate functional membrane domains. (sputnic-group.ru)
The global Cell Line and Membrane Market size was valued at $4.5 billion in 2021, and is projected to reach $10.6 billion by 2031, growing at a CAGR of 8.8% from 2022 to 2031. (alliedmarketresearch.com)
The overall impact of COVID-19 remains positive on Cell Line and Membrane market. (alliedmarketresearch.com)
Moreover, many government initiatives are conducted to reduce disease prevalence among animals which lead to growth of Cell Line and Membrane market. (alliedmarketresearch.com)
The key factors that drive the Cell Line and Membrane Market growth are rises in the prevalence of cancer and chronic diseases. (alliedmarketresearch.com)
It is unexpected that B. subtilis SQR without covalently bound FAD is not assembled in the E. coli membrane provided that all other aspects of assembly, including iron-sulfur cluster biogenesis, are functional in the heterologous system. (lu.se)

There are many options available for protein expression from cloned DNA. (thermofisher.com)
The variety of expression choices available makes the Gateway system of recombination cloning ideal for protein expression studies. (thermofisher.com)
The extremely wide variety of Gateway cloning-compatible expression vectors available makes the Gateway system of recombination cloning ideal for protein expression studies. (thermofisher.com)

Further, Agilent will be able to offer a set of catalogue components that will enable creation of more than 1 million different cloning vectors through SureVector cloning kits. (eurofinsgenomics.com)
The collaboration benefits the SureVector system as the process of combining functional DNA modules and assembling them in to vectors will be faster and easier than before. (eurofinsgenomics.com)

Various anionic drugs have been indicated to be taken up into the proximal tubular cells by the classic p -aminohippurate (PAH) transporter. (aspetjournals.org)
Glucose enters at the luminal side of the proximal tubular cells by an active carrier-mediated transport process that requires energy provided by the sodium gradient between the intra- and extracellular compartments generated by sodium-potassium ATPase. (medscape.com)

The manual screening methods traditionally used for cell line development are time-consuming and labor-intensive, creating a great demand for high throughput, automated solutions for such efforts. (moleculardevices.com)
Additionally, the invention provides methods of making the compositions, as well as methods of introducing the nucleic acids into cells using the compositions and treating disease conditions. (justia.com)
The present report gives an overview of the terms and methods used in cloning and summarizes the debates in the General Assembly. (who.int)

The purpose of the present study was to develop a mammalian expression system for hOCT1 and to characterize the interactions of various compounds with the cloned transporter. (drugbank.com)

Another aim is to examine the functional consequences of mutations of Cx50 and Cx46 found in congenital cataract patients. (rosalindfranklin.edu)

Of these, 17 were identical or homologous to known mammalian cDNAs or expressed sequence tags. (diabetesjournals.org)

Maximize efficiency, preserve cell viability, and streamline your workflow with DispenCell and CloneSelect Imager FL bundle. (moleculardevices.com)

In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

While more and more long intergenic non-coding RNAs (lincRNAs) were identified to take important roles in both maintaining pluripotency and regulating differentiation, how these lincRNAs may define and drive cell fate decisions on a global scale are still mostly elusive. (biomedcentral.com)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)

GNPDA1 siRNA induced GFAT2 which was hardly measurable in these cells under standard culture conditions, GNPDA2 siRNA increased GFAT1, and GFAT1 siRNA increased the expression of hyaluronan synthase 2 (HAS2). (nih.gov)
Analysis of heterologous expression systems of rOAT1 in oocytes and culture cells revealed that rOAT1 has the ability to transport anionic drugs, such as β-lactam antibiotics, NSAIDs, methotrexate, and antiviral drugs, as well as various endogenous organic anions and exogenous substances. (aspetjournals.org)
A different experimental approach by West and Cooke [ 10 ] demonstrated the secretion of M r 80-90K hCG-LH receptor complex into the culture media from ligand-induced rat and mouse leydig cells. (biomedcentral.com)
Cell line is a general term that applies to a defined population of cells that can be maintained in culture for an extended period of time, retaining stability of certain phenotypes and functions. (alliedmarketresearch.com)
A cell line is a permanently established cell culture that will multiply indefinitely given appropriate fresh medium and space and is frequently used to investigate biological processes in place of primary cells. (alliedmarketresearch.com)

Cell lines are usually clonal, meaning that the entire population originated from a single common ancestor cell. (alliedmarketresearch.com)

Gaussian online artificial photosynthesis in Human Cells Catalyzed by Phage polynucleotide Integrase Mutants, 2000 J. Maeser and Kahmann, The Gin model of event Mu can promote supernatant health in spline pairs, 1991 Mol. (scoutconnection.com)

The arginine decarboxylase (ADC) is a significant functional enzyme, synthesizes agmatine through arginine metabolism, and agmatine was reported to posses protective properties in various tissues. (elsevierpure.com)

The recovery of blood-forming stem cells, determined as endogenous spleen colonies, was markedly stimulated by priming irradiation. (go.jp)
The reduction in bone marrow death seems chiefly due to the stimulated recovery of blood-forming stem cells. (go.jp)
The stem cells might produce an unknown factor(s) that contributes to acquired radioresistance. (go.jp)
Based on multiple RNA-Seq datasets, we systematically identified 300 human embryonic stem cell lincRNAs (hES lincRNAs). (biomedcentral.com)
As the first global profiling and annotating of human embryonic stem cell lincRNAs, this work aims to provide a valuable resource for both experimental biologists and bioinformaticians. (biomedcentral.com)
Moreover, we surveyed and manually screened published hES RNA-Seq datasets in several public repositories, resulting in a list of 31 wild-type human embryonic stem cell samples. (biomedcentral.com)
C ancers initiate and develop from rare population of stem-like cells termed as cancer stem cells (CSC). (madridge.org)
Our goal is to identify and define the single normal and cancer stem cell of every human tissue in order to succeed in cancer and stem cell therapy, organ engineering, reconstructive and cosmetic surgery.The exact phenotype of CSC and their counterparts in normal mammary gland is not well characterized. (madridge.org)
He discovered several cancer and stem cell specific biomarkers. (madridge.org)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
Stem Cell Reports. (lu.se)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
We're thrilled to have Chad Cowan, an associate professor at Harvard University who is at the Harvard Stem Cell Institute. (medscape.com)
Chad and I have both been principal investigators on the induced pluripotent stem cell (IPSC) grant. (medscape.com)
I fell in love with the idea of human pluripotent stem cells and started surveying the United States and even Europe for who was playing in that area. (medscape.com)
At International Society for Stem Cell Research-the big stem cell meeting-Yamanaka revealed one more factor. (medscape.com)

February 12, 1987) observed inhibition of influenza virus in NIH 3T3 mouse cells genetically modified to express the mouse protein Mx. (justia.com)
In 1987 the sdhCAB operon of B. subtilis was cloned and sequenced. (lu.se)

Distinct types of cells are used to create different cell lines. (alliedmarketresearch.com)

How do cells control the secretion of mucin and insulin? (drugtargetreview.com)

Glucose enters the cell along with sodium, and sodium exits the cell at the basolateral side of the cell, which is sodium-independent and a facilitative transport requiring no energy. (medscape.com)

In particular, the loss of ALKBH6 in human pancreatic cancer cells increases alkylating agent-induced DNA damage and significantly decreases cell survival. (frontiersin.org)
Overall, our data suggest that ALKBH6 is required to maintain the integrity of the genome and promote cell survival of pancreatic cancer cells. (frontiersin.org)
The extremely low 5-year overall survival of patients is, in part, due to pancreatic cancer cells having several mechanisms of resistance to different chemotherapeutic treatments, one of which is their capacity to efficiently repair alkylating agent-induced DNA damage. (frontiersin.org)

Functional analysis showed that they were preferentially involved in several early-development related biological processes. (biomedcentral.com)
Functional analysis further demonstrated that hES lincRNAs were preferentially involved in multiple development processes including embryo development, ribosome biogenesis, and aging. (biomedcentral.com)
In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)

When combined with functional or immunological screening procedures, the approach will be useful for the isolation of β-cell-specific molecules for immunological and genetic investigations of β-cell function and pathology. (diabetesjournals.org)
A patented disposable tip ensures clean isolation of single cells and no cross contamination. (moleculardevices.com)
fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

Anatomy37

Organisms27

Diseases1

Chemicals and Drugs65

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes94

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care1

Proteins22

Genes14

Yeast4

Characterization4

Gene27

Antibody10

Assays1

Genomes1

Transfection4

Molecular4

Genomics2

Genomic7

Epithelial cells1

Biology4

CDNA sequences2

Antiviral activity1

Insect4

Vector2

Antibodies10

Embryonic stem1

Vitro1

Laboratory3

Mitochondria2

Replication2

Receptors4

Progenitor cell3

Tumorigenesis1

19971

19951

Construct2

Antigen3

Biol4

Plasmid3

Mutant5

Species3

Nucleus2

Membrane7

Protein expression3

Vectors2

Proximal tubular cells2

Methods3

Characterize1

Mutations1

Sequence1

Workflow1

Transcriptional1

Differentiation2

Culture5

Clonal1

Phage1

Tissues1

Stem20

19872

Lines1

Insulin1

Basolateral1

Pancreatic3

Development3

Isolation3